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WO2019207644A1 - Dispositif fluidique, dispositif de valve et dispositif de détection - Google Patents

Dispositif fluidique, dispositif de valve et dispositif de détection Download PDF

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Publication number
WO2019207644A1
WO2019207644A1 PCT/JP2018/016606 JP2018016606W WO2019207644A1 WO 2019207644 A1 WO2019207644 A1 WO 2019207644A1 JP 2018016606 W JP2018016606 W JP 2018016606W WO 2019207644 A1 WO2019207644 A1 WO 2019207644A1
Authority
WO
WIPO (PCT)
Prior art keywords
flow path
valve
base material
sheet material
hole
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2018/016606
Other languages
English (en)
Japanese (ja)
Inventor
直也 石澤
太郎 上野
遼 小林
哲臣 高崎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nikon Corp
Original Assignee
Nikon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nikon Corp filed Critical Nikon Corp
Priority to PCT/JP2018/016606 priority Critical patent/WO2019207644A1/fr
Priority to JP2020515339A priority patent/JP7192859B2/ja
Publication of WO2019207644A1 publication Critical patent/WO2019207644A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass

Definitions

  • the present invention relates to a fluid device, a valve device, and a detection device.
  • ⁇ -TAS is superior to conventional inspection devices in that it can be measured and analyzed with a small amount of sample, can be carried, and can be disposable at low cost. Furthermore, in the case of using an expensive reagent or in the case of testing a small amount of a large number of specimens, it has attracted attention as a highly useful method.
  • Non-Patent Document 1 A device including a flow path and a pump disposed on the flow path as a component of ⁇ -TAS has been reported (Non-Patent Document 1).
  • a plurality of solutions are injected into the channel, and the pump is operated to mix the plurality of solutions in the channel.
  • a first base material and a second base material joined at a joining surface are provided, and at least one of the first base material or the second base material joins both base materials.
  • the first substrate has a through-hole provided at a position facing the flow path, and the opening on the flow path side of the through-hole.
  • An elastic member that adjusts the flow of fluid in the flow path by deformation and a first surface that is opposite to the joint surface of the first base material so as to cover the opening of the through hole and be peelable
  • a fluid device including a valve having a formed sheet material, and a drive unit that is disposed between the sheet material and the elastic member and deforms the elastic member in the flow path direction.
  • the first substrate is positioned at a position facing at least one of the first base material and the second base material joined at the joining surface and opened to the joining surface.
  • a valve device is provided that includes an applying portion that applies a force to close the flow path to the valve portion when the is attached to the first surface.
  • a placement part for placing the fluid device of the first aspect of the present invention, a peeling part for peeling the sheet material, and a target substance in the flow path of the fluid device There are provided a liquid feeding part for feeding a solution containing the liquid and a detection part for detecting a target substance in the solution.
  • 1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment.
  • 1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment.
  • 1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment.
  • Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. The top view which showed typically the fluid device containing the valve apparatus of one Embodiment. Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment.
  • FIGS. 1 to 13 In the drawings used in the following description, in order to make the features easier to understand, the portions that become the features may be shown in an enlarged manner for convenience, and the dimensional ratios of the respective constituent elements may not be the same as the actual ones. I can't.
  • FIG. 1 is a cross-sectional view schematically showing a fluid device 1 including a valve device V.
  • the fluidic device 1 of the present embodiment includes a device that detects and inspects a sample substance that is a detection target included in a specimen sample by an immune reaction, an enzyme reaction, or the like.
  • the sample substance is, for example, a biomolecule such as nucleic acid, DNA, RNA, peptide, protein, extracellular vesicle.
  • the fluid device 1 includes a base material 5 and a valve device V.
  • the substrate 5 has a first substrate 6 and a second substrate 9.
  • the 1st base material 6 and the 2nd base material 9 of this embodiment are comprised from the resin material.
  • the resin material constituting the first base material 6 and the second base material 9 include polypropylene and polycarbonate.
  • the 1st base material 6 is comprised from a transparent material.
  • the material which comprises the 1st base material 6 and the 2nd base material 9 is not limited.
  • first base material 6 and the second base material 9 are described along the horizontal plane, and the first base material 6 is described as being disposed above the second base material 9. However, this only defines the horizontal direction and the vertical direction for convenience of explanation, and does not limit the orientation when the fluidic device 1 according to the present embodiment is used.
  • the first base material 6 and the second base material 9 are plate members extending along the horizontal direction.
  • the 1st base material 6 and the 2nd base material 9 are laminated
  • the second substrate 9 is laminated on the lower side of the first substrate 6.
  • the first base material 6 and the second base material 9 are joined using the lower surface 6a of the first base material 6 and the upper surface 9b of the second base material 9 as joint surfaces.
  • the base material 5 is manufactured by joining and integrating the first base material 6 and the second base material 9 by a joining means such as adhesion using an adhesive, thermal welding, ultrasonic welding, laser welding or the like.
  • the direction in which the first base material 6 and the second base material 9 are stacked is simply referred to as a stacking direction.
  • the stacking direction is the vertical direction.
  • the 1st base material 6 has the flow path 11 and the through-holes 35a and 35b.
  • the flow path 11 is a space formed in a tube shape or a cylindrical shape surrounded by the groove provided on the lower surface 6 a of the first base material 6 and the second base material 9.
  • the channel 11 accommodates a solution for performing the above-described detection / inspection.
  • the through holes 35 a and 35 b are arranged at positions facing both ends of the flow path 11 and penetrate the first base material 6 in the vertical direction.
  • the through holes 35 a and 35 b are, for example, an injection hole and an air hole for a solution stored in the flow path 11.
  • the diameter of the through holes 35a and 35b is preferably 0.1 to 3 mm.
  • the width of the flow path 11 examples include 0.01 to 100 mm, 0.05 to 50 mm, 0.1 to 10 mm, 0.1 to 5 mm, and 0.5 to 3 ⁇ m. Further, as an example, the width of the flow path 11 is 0.01 to 1000 ⁇ m, 0.05 to 1000 ⁇ m, 0.2 to 500 ⁇ m, 1 to 250 ⁇ m, and 10 to 200 ⁇ m. Considering the formability when the first substrate 6 is manufactured by injection molding, the width of the flow path 11 is preferably 0.05 to 5 mm.
  • the depth of the flow path 11 examples include 0.01 to 100 mm, 0.05 to 50 mm, 0.1 to 10 mm, 0.1 to 5 mm, and 0.5 to 3 ⁇ m. Further, as an example, the depth of the flow path 11 is 0.01 to 1000 ⁇ m, 0.05 to 1000 ⁇ m, 0.2 to 500 ⁇ m, 1 to 250 ⁇ m, and 10 to 200 ⁇ m. Considering the moldability when the first substrate 6 is manufactured by injection molding, the depth of the flow path 11 is preferably 0.05 to 3 mm.
  • the valve device V is provided in the second base material 9 and a valve portion Vc held in a valve holding hole (through hole) 34 that penetrates the first base material 6 in the vertical direction at a position facing the flow path 11.
  • a valve holding hole through hole
  • the recess 40 a sheet material 41 that is detachably attached to the upper surface (first surface) 6 b of the first base material 62, and a shaft portion (giving portion, provided on the valve portion Vc) Drive part) 42.
  • the valve portion Vc is provided at a lower end portion of an annular ring portion 44 provided in the valve holding hole 34.
  • the shaft portion 42 is a drive portion that deforms and drives the valve portion Vc.
  • it is a provision part which provides the force which deforms the elastic member which is the valve part Vc, and is a press part which presses an elastic member.
  • the valve part Vc is an elastic member.
  • the valve portion Vc is a valve made of a flexible resin film.
  • the opening on the flow path 11 side of the through hole 34 is closed by an elastic member that is the valve portion Vc.
  • the valve portion Vc is made of an elastic material and adjusts the flow of fluid in the flow path 11 by deformation. Examples of the elastic material that can be used for the valve portion Vc include rubber and elastomer resin.
  • a projecting portion 36 projecting in a hemispherical shape is provided on the lower surface of the valve portion Vc.
  • the depression 40 is disposed immediately below the valve portion Vc on the upper surface 9 b of the second base material 9.
  • the recess 40 is formed in a hemispherical shape.
  • the valve part Vc opens and closes the flow path 11 by deformation.
  • the valve part Vc operates perpendicularly to the flow path direction of the flow path 11 to open and close the flow path 11.
  • the valve portion Vc is elastically deformed downward, and the projecting portion 36 comes into contact with the recess 40 to close the flow path 11. Further, the valve portion Vc opens the flow path 11 as shown in FIG. 1 by the protrusion 36 being separated from the recess 40 by the elastic restoring force.
  • the shaft portion 42 has a pillar shape substantially perpendicular to the flow path 11.
  • the shaft part 42 is formed in such a length that the upper end protrudes from the upper surface 6 b of the first base material 6. Further, the length of the shaft portion 42 is such that the upper end surface is substantially flush with the upper surface 6b of the first base material 62 or the upper surface 6b when the valve portion V is elastically deformed and the protruding portion 36 comes into contact with the recess 40. It is a length protruding upward. That is, the length of the shaft portion 42 in the penetrating direction of the valve holding hole 34 projects from the upper surface 6b before the sheet material 41 is attached to the upper surface 6b, and after the sheet material 41 is attached to the upper surface 6b. Is a length to close the flow path 11 by deforming.
  • the height from the upper surface of the valve portion Vc to the lower surface of the sheet material 41 when the lower surface of the valve portion Vc comes into contact with the bottom surface of the flow path 11 and the height of the shaft portion 42 that is an application portion (drive portion) are: Except for the amount of deformation of the valve portion Vc due to elastic deformation, it is substantially the same. In other words, the difference obtained by subtracting the thickness of the valve portion Vc from the sum of the height from the bottom surface of the flow channel 11 to the ceiling surface of the flow channel 11 and the thickness of the first base member 6 and the height of the shaft portion 42 are elastic. Except for the amount of deformation of the valve portion Vc due to deformation, it is substantially the same.
  • the valve portion Vc, the protruding portion 36, and the shaft portion 42 are formed bodies that are integrally formed of the above-described flexible elastic material.
  • the sheet material 41 is used for stable storage of the solution stored in the flow path 11 and prevention of moisture absorption of the solution during storage and transportation of the fluid device 1 before performing the detection and inspection described above.
  • the sheet material 41 is formed of a thin film material such as a film.
  • the sheet material 41 is detachably attached to the upper surface 6b of the first base material 6.
  • the sheet material 41 may have an adhesive layer on the contact surface side with the upper surface 6 b of the first base material 6. Further, the sheet material 41 may be detachably attached to the upper surface 6b of the first base material 6 via an adhesive.
  • the sheet material 41 in the present embodiment has a size that covers the valve holding hole 34 and the openings of the through holes 35a and 35b.
  • the first base member 6, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are integrally formed molded bodies.
  • the first base material 6, the valve portion Vc, the projecting portion 36, and the shaft portion 42 are formed using the mold in which the first base material 6 is accommodated after the first base material 6 is molded.
  • the portion 36 and the shaft portion 42 are molded by two-color molding.
  • FIGS. 3 and 4 are schematic configuration diagrams showing a first embodiment of a system having a sticking device 60 for sticking the sheet material 41 to the fluid device 1 described above.
  • the system includes a roller device 61 as the sticking device 60.
  • the roller device 61 rolls on the upper surface 6 b of the first base material 6 via the sheet material 41.
  • the roller device 61 moves from the end portion on one side (right side in FIG. 3) to the other side ( It rolls on the upper surface 6b through the sheet material 41 until the left side in FIG. By being pressed by the roller device 61, the sheet material 41 is bonded to the upper surface 6b.
  • the shaft portion 42 is pushed downward. That is, by sticking the sheet material 41, the shaft portion 42 is pushed in a direction substantially perpendicular to the direction in which the fluid flows in the flow path.
  • the valve portion Vc is elastically deformed in a direction substantially perpendicular to the direction in which the fluid flows in the flow path, and the flow path 11 is closed by the protrusion 36 coming into contact with the recess 40.
  • the sheet material 41 is peeled from the upper surface 6b.
  • the sheet material 41 is pierced or broken in the through hole 35.
  • the force applied to the shaft portion 42 by the sheet material 41 is removed.
  • the valve portion Vc is separated from the recess 40 by the elastic restoring force, as shown in FIG. 1, and the flow path 11 is opened.
  • the valve portion Vc functions as an initial close valve (normally close valve). That is, when the sheet material 41 is attached to the upper surface 6b, the valve portion Vc is in a closed state in which the flow of the fluid in the flow path 11 is blocked, so that the flow path is stored and transported before detection and inspection. It can suppress that the solution accommodated in 11 leaks, or the solutions before mixing are mixed.
  • the sheet material 41 is peeled from the upper surface 6b, whereby the valve portion Vc is deformed into an open state in which the fluid in the flow path 11 flows, and the blockage of the flow path 11 is released. It is possible to perform detection and inspection using the fluid device 1 smoothly and stably after transportation.
  • the roller device 61 rolls on the upper surface 6b via the sheet material 41, whereby the sheet material 41 can be easily attached to the upper surface 6b. In particular, when the sheet material 41 covers all the openings of the through holes 34, 35a, and 35b, the sheet material 41 can be easily attached.
  • the sheet material 41 does not cover the entire upper surface 6b but may partially cover the openings on the upper surface 6b side of the through holes 34, 35a, and 35b. Further, the sheet materials 41 covering the openings of the through holes 34, 35a, and 35b may be different from each other. In this case, a small amount of sheet material is sufficient, and the through holes 34, 35a, and 35b can be released stepwise.
  • FIGS. 1 to 4 the same components as those of the first embodiment shown in FIGS. 1 to 4 are denoted by the same reference numerals, and the description thereof is omitted. Since the difference between the second embodiment and the first embodiment is the configuration of the sticking device 60, the sticking device 60 will be described below.
  • the fluid device 1 is packaged using the bag body 62 during storage and transportation before detection and inspection.
  • a region facing the upper surface 6 b of the bag body 62 is the sheet material 41.
  • a suction device 63 is provided as the sticking device 60. The suction device 63 sucks the inside of the bag body 62 through the opening of the bag body 62 under negative pressure.
  • the suction device 63 sucks the inside of the bag body 62 with a negative pressure, so that the pressure inside the bag body 62 becomes lower than the outside pressure.
  • the bag body 62 is pressed and attached to the outer surface of the base material 5 including the upper surface 6 b by atmospheric pressure.
  • the shaft portion 42 is pushed downward as described above.
  • the valve portion Vc is in a state in which the flow path 11 is closed by the protrusion 36 coming into contact with the recess 40.
  • the sealing portion 64 is provided at the mouth portion of the bag body 62 by welding or the like, whereby the blockage of the flow path 11 by the valve portion Vc is maintained. Further, when performing detection / inspection using the fluid device 1, the valve portion Vc has an elastic restoring force as shown in FIG. Thus, the channel 11 is released from the recess 40 and opened.
  • the fluid device 1 is packaged by the bag body 62 before detection and inspection. Contamination of the fluid device 1 with dust or the like before detection / inspection can be suppressed.
  • FIGS. 1 and 2 the same components as those of the first embodiment shown in FIGS. 1 and 2 are denoted by the same reference numerals, and the description thereof is omitted.
  • FIG. 7 is a schematic cross-sectional view of a detection apparatus 70 that performs detection using the fluidic device 1 according to the second embodiment.
  • FIG. 8 is a plan view schematically showing the fluidic device 1. In addition, in FIG. 8, it illustrates in the state which permeate
  • the detection device 70 includes a placement unit 71 for placing the fluid device 1, a peeling unit 72 for peeling the sheet material 41, and a liquid feeding unit 73 for feeding a solution containing the target substance to the flow path 11 of the fluid device 1. And a detection unit 74 that detects a target substance in the solution.
  • the fluid device 1 includes a base material 5.
  • the base material 5 has three board
  • the third base material 8 is formed of the same resin material as the first base material 6 and the second base material 9, and is disposed below the second base material 9.
  • the upper surface 8 b of the third base material 8 is joined to the lower surface 9 a of the second base material 9.
  • the base material 5 includes an injection hole 32, a reservoir 29, a flow path 11, a waste liquid tank 7, an air hole 35, a supply hole 39, a metering valve Vq, an introduction valve Vi, a discharge valve Vo, and the valves described above.
  • a device V is provided.
  • the injection hole 32 penetrates the first base material 6 and the second base material 9.
  • the injection hole 32 is connected to a reservoir 29 located at the boundary between the second base material 9 and the third base material 8.
  • the injection hole 32 connects the reservoir 29 to the outside.
  • the solution is filled into the reservoir 29 through the injection hole 32.
  • One injection hole 32 is provided for one reservoir 29. In FIG. 8, the injection hole 32 is not shown.
  • the reservoir 29 has a flow channel shape at least in the connection portion with the flow channel 11. For example, it is a space formed in a tubular shape or a cylindrical shape provided on the lower surface 9 a of the second base material 9.
  • the substrate 5 of the present embodiment is provided with a plurality of reservoirs 29.
  • the reservoir 29 stores a solution.
  • the plurality of reservoirs 29 contain solutions independently of each other.
  • the plurality of reservoirs 29 contain, for example, a plurality of reagents.
  • Each of the plurality of reservoirs 29 is provided with the above-described initial close valve.
  • the reservoir 29 supplies the stored solution to the flow path 11.
  • the reservoir 29 of this embodiment is a flow path type reservoir as an example. One end of the reservoir 29 in the length direction is connected to the injection hole 32. Further, the supply hole 39 is connected to the other end of the reservoir 29 in the length direction.
  • the reservoir 29 is provided on the second base material 9 and the opening is covered with the third base material 8 has been described.
  • the reservoir 29 may be configured to be provided in the third base material 8 and cover the opening with the second base material 9.
  • the flow path 11 is a space formed in a tube shape or a cylindrical shape provided on the upper surface 9 b of the second base material 9.
  • the solution is supplied to the flow path 11 from the reservoir 29 through the supply hole 39.
  • the solution flows in the flow path 11.
  • the supply hole 39 is provided in the second base material 9.
  • the supply hole 39 penetrates the second base material 9 in the plate thickness direction.
  • the supply hole 39 connects the reservoir 29 and the flow path 11.
  • the solution stored in the reservoir 29 is supplied to the flow path 11 through the supply hole 39. That is, the reservoir 29 is connected to the flow path 11 through the supply hole 39.
  • the waste liquid tank 7 is provided on the base material 5 in order to discard the solution in the flow path 11.
  • the waste liquid tank 7 is connected to the flow path 11.
  • the waste liquid tank 7 is disposed in the inner region of the circulation channel 10. Thereby, size reduction of the fluid device 1 can be achieved.
  • the waste liquid tank 7 is constituted by a concave stitch provided on the upper surface 9 b side of the second base material 9.
  • the air hole 35 is provided in the first base material 6.
  • the air hole 35 is located immediately above the waste liquid tank 7.
  • the air hole 35 connects the waste liquid tank 7 to the outside. That is, the waste liquid tank 7 is opened to the outside through the air hole 35.
  • the introduction valve Vi is disposed at a position facing the flow path 11 between the supply hole 39 and the waste liquid tank 7.
  • the introduction valve Vi has a valve portion Vd held in a valve holding hole 34 i that penetrates the first base material 6 in the vertical direction at a position facing the flow path 11.
  • the valve portion Vd is formed of an elastic material such as rubber or elastomer resin.
  • a projecting portion 36i projecting in a hemispherical shape is provided on the lower surface of the valve portion Vd.
  • a recess 40i is provided in the second base material 9 facing the valve portion Vd.
  • the valve portion Vd is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34i, and adjusts the flow of the solution in the flow path 11.
  • the valve portion Vd is supplied with driving fluid into the valve holding hole 34i and elastically deforms downward, and the projecting portion 36i contacts the recess 40i to close the flow path 11.
  • the valve portion Vd opens the flow path 11 by separating the protruding portion 36i from the recess 40i by an elastic restoring force.
  • the discharge valve Vo is disposed at a position facing the flow path 11 between the introduction valve Vi and the waste liquid tank 7.
  • the discharge valve Vo has a valve portion Vf held in a valve holding hole 34o penetrating the first base material 6 in the vertical direction at a position facing the flow path 11.
  • the valve portion Vf is formed of an elastic material such as rubber or elastomer resin.
  • a projecting portion 36o projecting in a hemispherical shape is provided on the lower surface of the valve portion Vf.
  • the second base material 9 facing the valve portion Vf is provided with a recess 40o.
  • the valve portion Vf is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34o and adjusts the flow of the solution in the flow path 11.
  • a driving fluid for example, air
  • the valve portion Vf is supplied with driving fluid into the valve holding hole 34o and elastically deforms downward, and the projecting portion 36o contacts the recess 40o to close the flow path 11.
  • the valve portion Vf opens the flow path 11 by separating the protruding portion 36i from the recess 40o by an elastic restoring force.
  • the metering valve Vq has a valve portion Ve held by a valve holding hole 34q that penetrates the first base material 6 in a vertical direction at a position facing the flow path 11.
  • the valve portion Ve is formed of an elastic material such as rubber or elastomer resin.
  • a projecting portion 36q projecting in a hemispherical shape is provided on the lower surface of the valve portion Ve.
  • the second base material 9 facing the valve portion Ve is provided with a recess 40q.
  • the valve portion Ve is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34q, and adjusts the flow of the solution in the flow path 11.
  • a driving fluid for example, air
  • the valve portion Ve is supplied with driving fluid into the valve holding hole 34q and elastically deforms downward, and the projecting portion 36q contacts the recess 40q to close the flow path 11.
  • the valve portion Ve opens the flow path 11 by separating the protruding portion 36q from the recess 40q by an elastic restoring force.
  • the valve device V described above is disposed at a position facing the flow path 11 between the supply hole 39 and the introduction valve Vi.
  • the first base material 6, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are formed integrally.
  • the first base material 6, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36 and the shaft portion 42 are accommodated in the first base material 6 after the first base material 6 is formed, for example.
  • the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are molded by the two-color molding using the molded mold.
  • one of the first base 6 manufactured in advance, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36 and the shaft portion 42 is mounted on a mold for molding the other. It is also possible to mold by insert molding.
  • the introduction valve Vi, the discharge valve Vo, and the metering valve Vq are initial open valves (second valves).
  • the initial close valve and the initial open valve described above are arranged in series in the flow path 11.
  • the sheet material 41 is attached to the upper surface 6b in a state where the solution is stored in the reservoir 29 before detection and inspection.
  • a high-viscosity reagent is accommodated in the flow path 11 between the introduction valve Vi and the discharge valve Vo as an example.
  • the injection hole 32 and the air hole 35 are closed by the sheet material 41.
  • the flow path 11 and the reservoir 29 are not released to the atmosphere. Therefore, the solution stored in the reservoir 29 is held in the reservoir 29 with the flow suppressed, and the reagent stored in the flow path 11 is held in the flow path 11 with the flow suppressed. Further, in the valve portion Vc, the projecting portion 36 abuts the recess 40, the flow path 11 is closed, and the reservoir 29 and the flow path 11 are separated. Therefore, even when a large acceleration is applied to the fluid device 1, It can suppress mixing with a reagent. Furthermore, in the fluid device 1 described above, since the air flow in the reservoir 29 and the flow path 11 is also suppressed, it is possible to suppress the evaporation of the solution and the reagent.
  • the channel 11 is not open to the atmosphere, the flow of the solution, reagent, and air in the reservoir 29 and the channel 11 is suppressed.
  • the flow path 11 includes a circulation flow path 10, a plurality (three in the example of FIG. 8) introduction flow paths 12, and a plurality (three in the example of FIG. 8) discharge flow paths 13. And including.
  • a solution is introduced into the channel 11 from a reservoir 29 (see FIG. 7).
  • the circulation channel 10 is configured in a loop shape when viewed from the stacking direction.
  • a pump P is disposed in the path of the circulation channel 10.
  • the pump P is composed of three element pumps Pe arranged side by side in the flow path.
  • the element pump Pe is a so-called valve pump.
  • the pump P can convey the liquid in the circulation channel by sequentially opening and closing the three element pumps Pe.
  • the number of element pumps Pe constituting the pump P may be four or more.
  • a plurality of (three in the example of FIG. 8) metering valves Vq are provided in the path of the circulation channel 10.
  • the plurality of metering valves Vq partitions the circulation channel 10 into a plurality of metering sections 18.
  • the plurality of metering valves Vq are arranged so that each metering section 18 has a predetermined volume.
  • a meandering portion 18 a is provided in one of the three quantitative sections 18.
  • the meandering portion 18a is a channel formed by meandering left and right.
  • the meandering portion 18a is provided in order to make one quantitative section 18 have a desired capacity.
  • Quantitative sections 18 each extend in a flow path shape.
  • the plurality of fixed-quantity sections 18 each have a flow path-shaped transfer flow path 80, an inflow portion 81 located at one end of the transfer flow path 80, and a merge portion provided at the other end of the transfer flow path 80. 85. Therefore, in each quantitative section 18, the transfer flow path 80 is located between the inflow portion 81 and the merging portion 85.
  • the inflow portion 81 of one quantitative section 18 is connected to the merging section 85 of another quantitative section 18 via a quantitative valve Vq.
  • the introduction flow path 12 is connected to the inflow portion 81 via the introduction valve Vi.
  • the merging section 85 of one quantitative section 18 is connected to an inflow section 81 of another quantitative section 18 via a quantitative valve Vq.
  • the discharge flow path 13 is connected to the junction 85 via a discharge valve Vo.
  • the introduction flow path 12 is a flow path for introducing the solution into the quantitative section 18 of the circulation flow path 10.
  • the introduction channel 12 is provided for each quantitative section 18 of the circulation channel 10.
  • the introduction channel 12 is connected to the supply hole 39 on one end side.
  • the introduction flow path 12 is connected to the inflow portion 81 of the circulation flow path 10 on the other end side.
  • the discharge channel 13 is a channel for discharging the solution in the quantitative section 18 of the circulation channel 10 to the waste liquid tank 7.
  • the discharge channel 13 is provided for each quantitative section 18 of the circulation channel 10.
  • the discharge channel 13 is connected to the waste liquid tank 7 at one end side. Further, the discharge flow channel 13 is connected to the merging portion 85 of the circulation flow channel 10 on the other end side.
  • the fluid device 1 In placing the fluid device 1 on the placement part 71, the fluid device 1 is positioned so that the lower surface 8 a of the third base material 8 in the fluid device 1 is in contact with the upper surface of the placement part 71.
  • the peeling portion 72 moves relative to the upper surface 6 b of the first base material 6 with one end of the sheet material 41 sucked, for example, under a negative pressure.
  • the material 41 is peeled from the upper surface 6b.
  • the placement step for example, when the lower surface 8a of the third base material 8 in the fluid device 1 is bonded to the upper surface of the placement portion 71 or the fluid device 1 is fixed to the placement portion 71. Is easy to peel.
  • the solution S stored in the reservoir 29 is sent to the flow path 11 by the liquid feeding unit 73 after the sheet material 41 is peeled from the fluid device 1.
  • the opening and closing of the valves Vq, Vi, and Vo which will be described later, is performed by switching the supply and stop of the supply of the driving fluid to the valve holding holes 34q, 34i, and 34o. Omitted.
  • the reservoir 29 is preliminarily held and / or filled with the solution S.
  • the sheet material 41 is peeled from the upper surface 6 b of the first base 6, whereby the protrusion 36 in the valve device V is separated from the recess 40 and the flow path 11 is formed. Opened.
  • the solution and the reagent are in a state in which evaporation and mixing are suppressed.
  • the solution S in the reservoir 29 is moved to the flow path 11. More specifically, the solution S is sequentially introduced from the reservoir 29 into each quantitative section 18 of the circulation channel 10.
  • the procedure for introducing the solution S into one quantitative section 18 will be described, but the solution S is also introduced by performing the same procedure for the other quantitative sections 18.
  • valves Vq, Vi, Vo when introducing the solution S into the quantitative section 18 will be described with reference to FIG.
  • the pair of metering valves Vq located on both sides in the length direction of the metering section 18 for introducing the solution S are closed.
  • the discharge valve Vo of the discharge flow path 13 connected to the corresponding quantitative section 18 is opened, and the discharge valves Vo of the other discharge flow paths 13 are closed.
  • the introduction valve Vi of the introduction flow path 12 connected to the corresponding quantitative section 18 is opened.
  • the detection unit 74 shown in FIG. 7 detects the solution mixed in the flow path 11.
  • the target substance is, for example, a sample substance included in a specimen sample.
  • the sample substance is, for example, a biomolecule such as a nucleic acid, DNA, RNA, peptide, protein, extracellular vesicle or particle.
  • substances mixed with the target substance in the solution include a labeling substance (detection auxiliary substance) that binds to the sample substance and assists the detection of the sample substance, and a substance that captures and collects the sample substance in the solution. .
  • the detection unit 74 for example, a configuration in which the target substance is imaged and detected via the first base material 6 by an imaging element, or a configuration in which the target substance is detected by measuring the magnetic force via the first base material 6 Can be taken.
  • Insert molding may be performed in which molding is performed in a state in which the metered valve Vq, the introduction valve Vi, the discharge valve Vo, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are stored.
  • the shaft portion 42 provided in the valve portion Vc is exemplified as the applying portion that applies the force for closing the flow path 11 to the valve portion.
  • the present invention is not limited to this configuration.
  • the granular member 42A loaded in the valve holding hole 34 between the seat material 41 and the valve portion Vc may be used.
  • the granular member 42A may have a cylindrical shape, a cubic shape or a rectangular parallelepiped shape in addition to the spherical shape shown in FIG.
  • the granular member 42A is preferably spherical from the viewpoint of maintaining the distance between the seat member 41 and the valve portion Vc at a predetermined value without being affected by the posture after being charged.
  • the granular member 42A attached to the sheet material 41 can be easily and collectively removed when the sheet material 41 having the adhesive layer on the lower surface is peeled off from the upper surface 6b. It can be removed.
  • the application portion is provided to protrude downward from the lower surface of the sheet material 41 as shown in FIG.
  • the structure used as the axial part 42B may be sufficient. Even in this configuration, since the application portion does not protrude from the upper surface 6b after the sheet material 41 is peeled off, for example, a recess for preventing interference with the application portion needs to be formed in the member for injecting the solution into the through hole 35a. Disappear.
  • valve portion Vc is a cylindrical shape attached to the inner peripheral surface of the valve holding hole 34
  • the present invention is not limited to this configuration.
  • a fitting convex portion 34 a that protrudes annularly radially inward is provided in the middle in the vertical direction on the inner peripheral surface of the valve holding hole 34, and the fitting convex portion 34 a is provided on the valve portion Vc.
  • the structure which provides the fitting recessed part 43 to fit may be sufficient. Since the valve portion Vc having this configuration is engaged with the fitting convex portion 34a from both sides in the vertical direction, the valve portion Vc is prevented from being detached from the first base material 6 when the sheet material 41 is attached. it can.
  • FIG. 12 is a plan view of the second base material 9 in which the flow path 11 is formed.
  • a recess 40 is formed in the upper surface 9b of the second base material 9 at a position facing the protruding portion 36 of the valve portion Vc, as shown in FIG.
  • the recess 40 is disposed in the middle of the flow path 11.
  • 13 is a cross-sectional view taken along line AA in FIG. FIG.
  • the bottom surface of the channel 11 is inclined upward from the position of the ridge line of the recess 40 in the upper surface 9b.
  • valve portion Vc is elastically deformed downward, and the projecting portion 36 abuts on the recess 40 to close the flow path 11.
  • the flow path 11 can be more reliably closed.
  • the depth of the flow path 11 is 0.05 mm or more and 3 mm or less (for example, 0.3 mm).
  • Examples of the diameter D of the recess around the shaft portion 42 include 1 mm or more and 5 mm or less (for example, 2.2 mm).
  • Examples of the diameter of the shaft portion 42 include 0.5 mm or more and 4 mm or less (for example, 1.0 mm).
  • Examples of the protruding amount of the shaft portion 42 from the upper surface 6b include 0.05 mm or more and 3 mm or less (for example, 0.4 mm).
  • the gap amount t between the lower end of the protrusion 36 and the lower end of the recess 40 when the valve portion Vc opens the flow path 11 is 0.05 mm or more and 1.0 mm or less (for example, 0.1 mm).
  • Examples of the diameter of the circle formed by the ridge line of the recess 40 in the upper surface 9b include 0.5 mm or more and 5.0 mm or less (for example, 2.0 mm).
  • Examples of the radius RA of the spherical surface of the recess 40 include 1.0 mm or more and 5.0 mm or less (for example, 1.45 mm).
  • Examples of the radius RB of the spherical surface of the protrusion 36 include 1.0 mm or more and 5.0 mm or less (for example, 1.82 mm).
  • bulb Vc has the protrusion part 36
  • valve part Vc illustrated the structure which opens and closes the flow path 11 by sticking and peeling of the sheet
  • the flow of the flow path 11 is controlled by supplying and stopping the supply of the driving fluid to the valve holding hole 34 (solution flow adjustment ) May be switched.
  • the upper end of the provision part was illustrated in the same position as the upper surface 6b, However, It is not limited to this structure, Affixed The upper end of the applying portion may protrude from the upper surface 6b as long as the sheet material 41 does not peel off.
  • seat material 41 has an adhesive layer was illustrated, it is not limited to this structure, For example, a sheet
  • the structure which affixes material on the upper surface 6b may be sufficient.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
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Abstract

L'objectif de la présente invention est de fournir un dispositif fluidique capable de donner des résultats de détection et d'inspection stables après stockage et transport. La présente invention comprend un premier substrat et un second substrat joints au niveau d'une surface de jonction. Au moins l'un parmi le premier substrat et le second substrat comporte, au niveau de la surface de jonction, une rainure qui forme un trajet d'écoulement suite à l'assemblage des substrats. Le premier substrat comprend une valve comportant : un trou traversant disposé au niveau d'une position située à l'opposé du trajet d'écoulement ; un élément élastique qui bloque l'ouverture latérale de trajet d'écoulement du trou traversant et régule l'écoulement du fluide à travers le trajet d'écoulement par déformation ; un élément en forme de feuille qui est fixé de manière détachable à une première surface, qui se situe du côté opposé à la surface de jonction sur le premier substrat, de façon à recouvrir l'ouverture du trou traversant ; et une unité d'entraînement qui est disposée entre l'élément en forme de feuille et l'élément élastique et qui déforme l'élément élastique dans la direction du trajet d'écoulement.
PCT/JP2018/016606 2018-04-24 2018-04-24 Dispositif fluidique, dispositif de valve et dispositif de détection Ceased WO2019207644A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/JP2018/016606 WO2019207644A1 (fr) 2018-04-24 2018-04-24 Dispositif fluidique, dispositif de valve et dispositif de détection
JP2020515339A JP7192859B2 (ja) 2018-04-24 2018-04-24 流体デバイス、バルブ装置及び検出装置

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2018/016606 WO2019207644A1 (fr) 2018-04-24 2018-04-24 Dispositif fluidique, dispositif de valve et dispositif de détection

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150093815A1 (en) * 2013-09-30 2015-04-02 Gnubio, Inc. Microfluidic cartridge devices and methods of use and assembly
WO2016006615A1 (fr) * 2014-07-07 2016-01-14 国立大学法人東京大学 Valve, dispositif fluidique, procédé de commande de fluide, et procédé de fabrication de valve
WO2016136551A1 (fr) * 2015-02-25 2016-09-01 国立大学法人東京大学 Vanne, dispositif à fluide et procédé de production de dispositif à fluide
US20170080422A1 (en) * 2014-05-16 2017-03-23 Qvella Corporation Apparatus, system and method for performing automated centrifugal separation
WO2018012429A1 (fr) * 2016-07-13 2018-01-18 株式会社ニコン Dispositif fluidique, procédé de fabrication d'un dispositif fluidique et robinet pour dispositif fluidique

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY188606A (en) * 2015-07-09 2021-12-22 Vistadeltek Llc Control plate in a valve

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150093815A1 (en) * 2013-09-30 2015-04-02 Gnubio, Inc. Microfluidic cartridge devices and methods of use and assembly
US20170080422A1 (en) * 2014-05-16 2017-03-23 Qvella Corporation Apparatus, system and method for performing automated centrifugal separation
WO2016006615A1 (fr) * 2014-07-07 2016-01-14 国立大学法人東京大学 Valve, dispositif fluidique, procédé de commande de fluide, et procédé de fabrication de valve
WO2016136551A1 (fr) * 2015-02-25 2016-09-01 国立大学法人東京大学 Vanne, dispositif à fluide et procédé de production de dispositif à fluide
WO2018012429A1 (fr) * 2016-07-13 2018-01-18 株式会社ニコン Dispositif fluidique, procédé de fabrication d'un dispositif fluidique et robinet pour dispositif fluidique

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