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WO2019203633A3 - Gène recombinant - Google Patents

Gène recombinant Download PDF

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Publication number
WO2019203633A3
WO2019203633A3 PCT/MY2019/000010 MY2019000010W WO2019203633A3 WO 2019203633 A3 WO2019203633 A3 WO 2019203633A3 MY 2019000010 W MY2019000010 W MY 2019000010W WO 2019203633 A3 WO2019203633 A3 WO 2019203633A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
recombinant gene
seq
amino acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/MY2019/000010
Other languages
English (en)
Other versions
WO2019203633A2 (fr
Inventor
Kim Chai LEE
Boon Tatt NG
Chuen Yi FOONG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kimagri Corp Sdn Bhd
Original Assignee
Kimagri Corp Sdn Bhd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kimagri Corp Sdn Bhd filed Critical Kimagri Corp Sdn Bhd
Priority to CN201980040421.8A priority Critical patent/CN112313338A/zh
Publication of WO2019203633A2 publication Critical patent/WO2019203633A2/fr
Publication of WO2019203633A3 publication Critical patent/WO2019203633A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un gène recombinant pour améliorer la résistance aux herbicides, aux bactéries et aux champignons et pour fournir des caractéristiques agronomiques améliorées, le gène recombinant étant caractérisé par des acides nucléiques comprenant un premier gène EPSPS avec une séquence d'acides aminés de SEQ ID No 1 et un second gène EPSPS avec une séquence d'acides aminés de SEQ ID No 2, le gène recombinant étant fusionné avec un gène codant pour un facteur de transcription avec une séquence d'acides aminés de SEQ ID No 3.
PCT/MY2019/000010 2018-04-18 2019-03-29 Gène recombinant Ceased WO2019203633A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201980040421.8A CN112313338A (zh) 2018-04-18 2019-03-29 重组基因

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2018701522A MY195997A (en) 2018-04-18 2018-04-18 Recombinant Gene
MYPI2018701522 2018-04-18

Publications (2)

Publication Number Publication Date
WO2019203633A2 WO2019203633A2 (fr) 2019-10-24
WO2019203633A3 true WO2019203633A3 (fr) 2019-11-28

Family

ID=68240247

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2019/000010 Ceased WO2019203633A2 (fr) 2018-04-18 2019-03-29 Gène recombinant

Country Status (3)

Country Link
CN (1) CN112313338A (fr)
MY (1) MY195997A (fr)
WO (1) WO2019203633A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111153976A (zh) * 2020-01-17 2020-05-15 沈阳农业大学 一种水稻防御机制调控蛋白质及其在育种中的应用
CN111187779B (zh) * 2020-02-14 2022-06-10 西南大学 抗病基因OsRLR1、转录因子OsWRKY19以及在水稻抗白叶枯病育种中的应用
CN112725351B (zh) * 2021-03-23 2022-11-11 上海师范大学 基因OsWRKY43在水稻抗白叶枯病中的应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006126671A1 (fr) * 2005-05-26 2006-11-30 National Institute Of Agrobiological Sciences Amelioration de la resistance aux maladies d'une plante au moyen de l'introduction d'un gene de facteur de transcription
CN101003808A (zh) * 2006-12-05 2007-07-25 中国科学院西双版纳热带植物园 水稻wrky45基因及其制备方法和应用
US9434958B2 (en) * 2011-03-10 2016-09-06 National Institute Of Agrobiological Sciences Complex disease resistant monocot having optimized agronomic characteristics
CN106467909A (zh) * 2015-08-14 2017-03-01 中国科学院遗传与发育生物学研究所 一种通过核苷酸定点替换获得抗草甘膦水稻的方法
US9605271B2 (en) * 2013-01-23 2017-03-28 National Institute Of Agrobiological Sciences Disease resistant plant expressing WRKY45 under control of infection-responsive promoter

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1384192A (zh) * 2001-05-08 2002-12-11 中国科学院遗传研究所 水稻5－烯醇丙酮莽草酸－3－磷酸合成酶以及编码该酶的基因
CN101386856B (zh) * 2008-10-21 2011-02-09 华中农业大学 水稻抗病相关基因OsWRKY45-2和它在改良水稻抗病性中的应用
CN103013939B (zh) * 2012-12-25 2015-01-21 北京大北农科技集团股份有限公司 除草剂抗性蛋白质、其编码基因及用途
PE20231848A1 (es) * 2015-07-22 2023-11-21 Monsanto Technology Llc Metodos y composiciones para la regulacion selectiva de la expresion de proteinas

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006126671A1 (fr) * 2005-05-26 2006-11-30 National Institute Of Agrobiological Sciences Amelioration de la resistance aux maladies d'une plante au moyen de l'introduction d'un gene de facteur de transcription
CN101003808A (zh) * 2006-12-05 2007-07-25 中国科学院西双版纳热带植物园 水稻wrky45基因及其制备方法和应用
US9434958B2 (en) * 2011-03-10 2016-09-06 National Institute Of Agrobiological Sciences Complex disease resistant monocot having optimized agronomic characteristics
US9605271B2 (en) * 2013-01-23 2017-03-28 National Institute Of Agrobiological Sciences Disease resistant plant expressing WRKY45 under control of infection-responsive promoter
CN106467909A (zh) * 2015-08-14 2017-03-01 中国科学院遗传与发育生物学研究所 一种通过核苷酸定点替换获得抗草甘膦水稻的方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE NUCLEOTIDE 12 October 2015 (2015-10-12), ANONYMOUS: "Oryza sativa Indica Group cultivar Swarna 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) mRNA, complete cds", XP055655745, retrieved from NCBI Database accession no. KR135155 *
DATABASE NUCLEOTIDE 2 March 2012 (2012-03-02), ANONYMOUS: "Expression vector pMON82053, complete sequence", XP055655750, retrieved from NCBI Database accession no. JN400387 *
DATABASE NUCLEOTIDE 28 July 2006 (2006-07-28), ANONYMOUS: "Oryza sativa WRKY transcription factor 45 mRNA, complete cds", XP055655735, retrieved from NCBI Database accession no. DQ298181 *
SHIMONO, M. ET AL.: "Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance", THE PLANT CELL, vol. 19, no. 6, June 2007 (2007-06-01), pages 2064 - 2076, XP055655755 *

Also Published As

Publication number Publication date
CN112313338A (zh) 2021-02-02
WO2019203633A2 (fr) 2019-10-24
MY195997A (en) 2023-02-27

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