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WO2019126920A1 - Dispositif de stimulation cellulaire et procédé de stimulation cellulaire - Google Patents

Dispositif de stimulation cellulaire et procédé de stimulation cellulaire Download PDF

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Publication number
WO2019126920A1
WO2019126920A1 PCT/CN2017/118212 CN2017118212W WO2019126920A1 WO 2019126920 A1 WO2019126920 A1 WO 2019126920A1 CN 2017118212 W CN2017118212 W CN 2017118212W WO 2019126920 A1 WO2019126920 A1 WO 2019126920A1
Authority
WO
WIPO (PCT)
Prior art keywords
stimulation
positioning seat
positioning
base
hole
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2017/118212
Other languages
English (en)
Chinese (zh)
Inventor
李永川
郭瑞彪
唐彩云
钱明
蔡飞燕
黄继卿
郑海荣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Institute of Advanced Technology of CAS
Original Assignee
Shenzhen Institute of Advanced Technology of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Institute of Advanced Technology of CAS filed Critical Shenzhen Institute of Advanced Technology of CAS
Priority to PCT/CN2017/118212 priority Critical patent/WO2019126920A1/fr
Publication of WO2019126920A1 publication Critical patent/WO2019126920A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Definitions

  • the invention belongs to the technical field of cell stimulation, and in particular relates to a cell stimulation device and a cell stimulation method.
  • nerve stimulation mainly includes drugs and external stimulation methods.
  • External stimulation methods include light stimulation and electrical stimulation.
  • Ultrasound stimulation is gaining more and more attention due to its safety, non-invasiveness, effectiveness and real-time.
  • Many laboratories in the world are currently conducting ultrasound-stimulated nerve cell research, and ultrasonic transducers are the core components. In the prior art, it is difficult to ensure that the ultrasound effect of cells received in each cell and group and between groups is Uniform, the experimental results are not good.
  • the object of the present invention is to overcome the above deficiencies of the prior art, and to provide a cell stimulation device and a cell stimulation method, which have good experimental results, and are precise, simple, convenient, and uniform.
  • the technical solution of the present invention is: a cell stimulation device comprising a stimulation probe, a base and a positioning seat slidable relative to the base, the base being provided with a plurality of holes for mounting the stimulation probe, the positioning The seat has a positioning structure for positioning the culture dish, the stimulation probe is mounted on the hole position and faces the positioning seat, and a guide bar sliding structure is disposed between the base and the positioning seat.
  • the guide bar sliding structure comprises a guiding rod, one end of the guiding rod is fixedly connected to the base, the positioning seat is provided with a sleeve hole, and the sleeve hole is sleeved on the guiding rod.
  • the positioning seat or the guiding rod is provided with a positioning structure for locking the positioning seat.
  • the positioning structure includes a threaded hole disposed on a side of the positioning seat and an adjusting bolt screwed to the threaded hole, the threaded hole is in communication with a side surface of the sleeve hole, and a front end of the adjusting bolt Abutting the side of the guide rod.
  • the hole position is a stepped hole.
  • the positioning structure is a receiving groove disposed on an upper surface of the positioning seat and configured to place the culture dish, and a bottom surface of the receiving groove is attached to a bottom surface of the culture dish.
  • a side of the positioning seat is screwed with a fixing bolt for fixing the petri dish.
  • the hole position is provided in plurality, and each of the hole positions is provided with an ultrasonic stimulation probe, and each of the ultrasonic stimulation probes is connected with a separate cable;
  • the culture dish has an array hole The array aperture is disposed coaxially with the hole for mounting the stimulation probe.
  • the side of the positioning seat is provided with a sound absorbing material for absorbing boundary ultrasonic waves.
  • the present invention also provides a cell stimulation method using the above cell stimulation device comprising the following steps:
  • the distance of the positioning seat relative to the stimulation probe is adjusted by a guide bar sliding structure.
  • the invention provides a cell stimulation device and a cell stimulation method.
  • the cells can be stimulated by controlling the size and intensity of the uniform ultrasonic stimulation of the cells in the culture dish.
  • the experiment has good effect, and the operation is precise, simple, convenient and quick, and can stimulate multiple groups of cells at the same time, and the uniformity is good.
  • FIG. 1 is a perspective exploded view of a cell stimulation device according to an embodiment of the present invention.
  • FIG. 2 is a schematic diagram of circuit connection of a cell stimulation device according to an embodiment of the present invention.
  • FIG. 3 is a three-dimensional assembly diagram of a base and a guide rod in a cell stimulation device according to an embodiment of the present invention
  • FIG. 4 is a schematic perspective view of a stimulation probe, a base, and a guide rod in a cell stimulation device according to an embodiment of the present invention
  • FIG. 5 is a three-dimensional assembly diagram of a stimulation probe, a base, a guide rod, and a positioning seat in a cell stimulation device according to an embodiment of the present invention
  • FIG. 6 is a schematic perspective view of a cell stimulation device according to an embodiment of the present invention.
  • left, right, upper, lower, and the like orientations in the embodiments of the present invention are merely relative concepts or referenced to the normal use state of the product, and should not be considered as limiting. .
  • a cell stimulation device includes a stimulation probe 1 (ultrasonic stimulation probe), a base 2, a petri dish 5, and a positioning seat 3 that can slide relative to the base 2, and the base 2
  • a stimulation probe 1 (ultrasonic stimulation probe), a base 2, a petri dish 5, and a positioning seat 3 that can slide relative to the base 2, and the base 2
  • a plurality of hole positions 11 for mounting the stimulation probe 1 are provided, and the stimulation probe 1 can be actively inserted into the hole position 11 and positioned by the hole position 11, that is, the stimulation probe 1 can be replaced.
  • the hole positions 11 can be arranged in a matrix.
  • the positioning seat 3 has a positioning structure for positioning the culture dish 5 so that the culture dish 5 can be smoothly placed on the positioning seat 3.
  • the stimulation probe 1 is mounted on the hole position 11 and faces the bottom of the positioning seat 3 and the culture dish 5.
  • the guide rod sliding structure is arranged between the base 2 and the positioning seat 3, so that the positioning seat 3 and the culture dish 5 can stably slide up and down. Thereby adjusting the distance between the culture dish 5 and the stimulation probe 1, the positioning seat 3 does not slide left and right or back and forth, by matching the array of stimulation probes 1 of different sizes, and by adjusting the height of the positioning seat 3, it can be controlled by the culture dish 5
  • the cells are stimulated by the size and intensity of the uniform ultrasonic stimulation.
  • the culture dish 5 can be provided with a plurality of culture chambers 51, and the plurality of culture chambers 51 can correspond to the arrangement of the holes 11 and the array of stimulation probes 1, that is, the chambers 51
  • the bottom has a coaxial hole position 11 and a corresponding stimulation probe 1, so that multiple groups of cells can be stimulated at the same time, the uniformity is good, the operation is simple and convenient, and the scope of use is very wide.
  • a plurality of hole positions 11 are provided, and one ultrasonic stimulation probe 1 is disposed in each hole position 11, and each (group) ultrasonic stimulation probe 1 is connected with a separate cable (coaxial cable).
  • Each (group) of ultrasonic stimulation probes 1 is connected to a power amplifier, each power amplifier is connected to a frequency divider, the frequency divider is connected to a DA module (digital-to-analog conversion module), and the DA module is connected to a signal source.
  • the guiding rod sliding structure comprises a guiding rod 4, one end of the guiding rod 4 is fixedly connected to the base 2, the positioning seat 3 is provided with a sleeve hole 31, and the sleeve hole 31 is slidably sleeved on the guiding rod 4.
  • the positioning seat 3 can be precisely slid up and down along the guide rod 4.
  • the guide bar 4 can be provided with at least two. In the embodiment, the guide bar 4 is provided with four, and the positioning precision is high, the position is accurate, the force is balanced, and the operation is simple and convenient.
  • the guide rod 4 can have a cylindrical shape. In a specific application, the number and shape of the guide bars 4 can be set according to actual conditions.
  • the guide rod 4 can also be fixed to the positioning base 3 and slidably inserted into the base 2, and the positioning base 3 can also be adjusted to slide up and down with respect to the base 2.
  • the positioning seat 3 or the guide bar 4 is provided with a positioning structure for locking the positioning seat 3 so that the positioning seat 3 can be temporarily positioned to be maintained at a set height.
  • the positioning structure includes a threaded hole disposed on the side of the positioning seat 3 and an adjusting bolt 61 threadedly connected to the threaded hole.
  • the threaded hole communicates with the side surface of the sleeve hole 31, and the front end of the adjusting bolt 61 can abut against the side of the guiding rod 4. Therefore, the positioning seat 3 can be fixed relative to the guide rod 4.
  • the adjusting bolt 61 is reversely loosened to release the leading end of the adjusting bolt 61.
  • the hole position 11 may be a stepped hole to position the stimulation probe 1 of different specifications, and the cell stimulation device has wide applicability.
  • the positioning structure is disposed on the upper surface of the positioning base 3 and is used for placing the accommodating groove 32 of the culture dish 5.
  • the bottom surface of the accommodating groove 32 is attached to the bottom surface of the culture dish 5 to make the culture dish 5 and the stimulation probe.
  • the surface of 1 is parallel.
  • the bottom of the accommodating groove 32 is provided with a hollow hole 33, so that the stimulation probe 1 can directly face the bottom of the culture dish 5, and the energy loss is small.
  • two opposite sides of the positioning seat 3 are screwed with fixing bolts 62 for fixing the culture dish 5 to adjust and fix the planar position of the culture dish 5, so that the central axis of the culture chamber 51 in the culture dish 5 and the stimulation probe The center axis of 1 coincides.
  • the side of the positioning seat 3 is provided with a sound absorbing material for absorbing boundary ultrasound.
  • the embodiment of the invention further provides a cell stimulation method, which comprises the above cell stimulation device, comprising the following steps:
  • the stimulation probe 1 is disposed upward in the hole 11 of the base 2 and faces the positioning seat 3;
  • the distance of the positioning seat 3 relative to the stimulation probe 1 is adjusted by the sliding structure of the guide rod 4, and ultrasonic stimulation is applied to the culture dish 5 by the stimulation probe 1.
  • the guide rod 4 can be firstly fixed on the base 2, as shown in FIG. 3, the base 2 is provided with an array of hole positions 11, and the hole position 11 has a positioning step for mounting the stimulation probe 1 group to ensure The flatness and parallelism of the upper surface of the base 2 and the steps of the array holes.
  • the guide rod 4 can be used to engage the positioning seat 3, so that the positioning seat 3 is not offset left and right, and does not rotate and can move up and down.
  • the stimulation probe 1 is then mounted on the hole 11 of the base 2 as shown in FIG.
  • the array of the stimulation probe 1 is installed in one-to-one correspondence with the hole position 11 of the array, and the stimulation probe 1 is matched with the step surface to ensure the parallelism between the probe surface and the upper surface of the base 2.
  • Each of the stimulation probes 1 is led out by a coaxial cable. .
  • the positioning seat 3 is then mounted on the guide bar 4 as shown in FIG.
  • the boundary of the positioning seat 3 can use a sound absorbing material to absorb the boundary ultrasonic to ensure the consistency of the ultrasonic stimulation field, and the small gap between the sleeve hole 31 (round hole) of the positioning seat 3 and the guide rod 4 ensures the positioning seat 3 and The parallelism of the surface of the probe; the positioning seat 3 moves up and down along the upper surface of the guide rod 4 with respect to the base 2, and the relative height is positioned by the adjusting bolt 61 (screw).
  • the cell culture dish 5 was installed as shown in FIG.
  • the culture dish 5 is placed on the positioning base 3, and by moving left and right, the culture chamber 51 of the array on the culture dish 5 is in one-to-one correspondence with the hole position 11 of the array on the base 2, and the bottom surface of the culture dish 5 is matched with the surface of the positioning seat 3 to ensure The corresponding culture chamber 51 is coaxial with the stimulation probe 1.
  • the culture dish 5 is secured by a fixing bolt 62 (screw).
  • the parallelism of the base 2, the positioning block 3, the stimulation probe 1, and the culture dish 5 is good, and the ultrasonic field of the stimulation of the stimulation probe 1 is uniformly applied to the cells in the culture dish 5.
  • multiple sets of stimulation probes 1 are used, and each set of stimulation probes 1 is extracted by using a coaxial line.
  • Each group of stimulation probes 1 can be connected with a power amplifier, which can stimulate multiple groups of cells at the same time, and the experiment is more convenient and quick. More sexual.
  • the size of the base 2, the positioning seat 3 and the like are not limited, and the size of the probe is not limited.
  • the probes of different sizes by adjusting the height of the positioning seat 3, the area of the cells in the culture dish 5 subjected to uniform ultrasonic stimulation can be controlled.
  • the intensity, the experimental effect is good, and the operation is precise, simple, convenient and fast, and can stimulate multiple groups of cells at the same time, and the uniformity is good.
  • the cell stimulation device and the cell stimulation method provided by the embodiments of the present invention can control the area of the cells in the culture dish 5 to be uniformly stimulated by adjusting the height of the positioning block 3 by matching the arrays of the stimulation probes 1 of different sizes.
  • the culture dish 5 can be provided with a plurality of culture chambers 51, and the plurality of culture chambers 51 can correspond to the arrangement of the holes 11 and the array of stimulation probes 1, that is, the coaxial holes are arranged under the respective culture chambers 51. 11 and the corresponding stimulation probe 1, so that multiple groups of cells can be stimulated at the same time, the uniformity is good, the operation is simple and convenient, and the application range is wide.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif de stimulation cellulaire et un procédé de stimulation cellulaire. Le dispositif de stimulation cellulaire comprend des sondes de stimulation (1), une base (2) et une douille de positionnement (3) pouvant coulisser par rapport à la base (2) ; la base (2) est pourvue d'une pluralité de sites de trous (11) pour monter les sondes de stimulation (1) ; la douille de positionnement (3) a une structure de positionnement pour positionner une boîte de culture (5) ; les sondes de stimulation (1) sont montées dans les sites de trous (11) et font face à la douille de positionnement (3) ; et une structure coulissante de barre de guidage est disposée entre la base (2) et la douille de positionnement (3). Le procédé de stimulation de cellule est mis en œuvre au moyen du dispositif de stimulation cellulaire ; la taille de la zone et l'intensité de stimulation ultrasonore uniforme reçue par des cellules dans la boîte de culture (5) sont commandées par adaptation d'un réseau de sondes de stimulation (1) de tailles différentes et l'ajustement d'une hauteur de la douille de positionnement (3), de manière à stimuler les cellules ; de multiples groupes de cellules peuvent être stimulés simultanément ; l'effet d'expérience est excellent, l'opération est précise, simple et rapide, et l'uniformité est bonne.
PCT/CN2017/118212 2017-12-25 2017-12-25 Dispositif de stimulation cellulaire et procédé de stimulation cellulaire Ceased WO2019126920A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/118212 WO2019126920A1 (fr) 2017-12-25 2017-12-25 Dispositif de stimulation cellulaire et procédé de stimulation cellulaire

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/118212 WO2019126920A1 (fr) 2017-12-25 2017-12-25 Dispositif de stimulation cellulaire et procédé de stimulation cellulaire

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WO2019126920A1 true WO2019126920A1 (fr) 2019-07-04

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699711B1 (en) * 1998-05-07 2004-03-02 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Device and method for selective exposure of a biological sample to sound waves
CN101892154A (zh) * 2010-08-03 2010-11-24 北京航空航天大学 一种压力-电联合刺激细胞培养装置
CN204151349U (zh) * 2014-09-01 2015-02-11 南京先欧仪器制造有限公司 一种大肠杆菌超声波破碎装置
CN106661535A (zh) * 2014-01-21 2017-05-10 普罗美迪卡生物电子学公司 用于超声波测试的设备
CN206512220U (zh) * 2016-11-23 2017-09-22 中国人民解放军第二军医大学 一种适用于n孔板的多元超声探头固定工作平台

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699711B1 (en) * 1998-05-07 2004-03-02 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Device and method for selective exposure of a biological sample to sound waves
CN101892154A (zh) * 2010-08-03 2010-11-24 北京航空航天大学 一种压力-电联合刺激细胞培养装置
CN106661535A (zh) * 2014-01-21 2017-05-10 普罗美迪卡生物电子学公司 用于超声波测试的设备
CN204151349U (zh) * 2014-09-01 2015-02-11 南京先欧仪器制造有限公司 一种大肠杆菌超声波破碎装置
CN206512220U (zh) * 2016-11-23 2017-09-22 中国人民解放军第二军医大学 一种适用于n孔板的多元超声探头固定工作平台

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