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WO2019187996A1 - Dispositif de distribution et procédé de distribution - Google Patents

Dispositif de distribution et procédé de distribution Download PDF

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Publication number
WO2019187996A1
WO2019187996A1 PCT/JP2019/008228 JP2019008228W WO2019187996A1 WO 2019187996 A1 WO2019187996 A1 WO 2019187996A1 JP 2019008228 W JP2019008228 W JP 2019008228W WO 2019187996 A1 WO2019187996 A1 WO 2019187996A1
Authority
WO
WIPO (PCT)
Prior art keywords
dispensing
amount
syringe pump
liquid
backlash
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2019/008228
Other languages
English (en)
Japanese (ja)
Inventor
匡章 平野
晃啓 安居
昌史 深谷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi High Tech Corp
Original Assignee
Hitachi High Technologies Corp
Hitachi High Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Technologies Corp, Hitachi High Tech Corp filed Critical Hitachi High Technologies Corp
Publication of WO2019187996A1 publication Critical patent/WO2019187996A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Definitions

  • the present invention relates to a dispensing device for dispensing a liquid.
  • Dispensing devices mounted on automatic analyzers that perform qualitative and quantitative analysis of biological samples such as blood and urine are required to dispense samples and reagents with high accuracy.
  • the dispensing device of the automatic analyzer is equipped with a nozzle that sucks and discharges the sample / reagent to be dispensed from the opening at the tip, and a plunger inserted into the syringe tube connected by the nozzle and tube.
  • a syringe pump that changes the volume and performs suction and discharge is provided. Since these components have dimensional errors caused by processing variations, there are machine differences in the dispensing apparatus.
  • Such a machine difference causes variations in the dispensed amount between the devices and fluctuations in the dispensed amount at the time of parts replacement, and affects the measured value of the analyzer.
  • the amount of samples and reagents has been reduced, and such variations and fluctuations cannot be ignored.
  • Patent Document 1 in order to eliminate the machine difference due to the difference in the inner diameter of the probe, the liquid sample is dispensed from the regular probe under the same conditions as the reference dispensing amount when the liquid sample is dispensed from the reference probe.
  • a dispensing amount correction method is proposed in which a difference from the dispensing amount is acquired and the drive condition is increased or decreased by an amount corresponding to the difference.
  • Patent Document 1 can eliminate an error in the dispensing amount due to the instrumental error of the probe.
  • the error of the dispensing amount due to the machine difference of the syringe pump is not necessarily fully considered.
  • a gap exists in a connecting part of mechanical parts such as a feed screw, a gear, and a timing belt of a syringe pump, and due to the gap, an idling operation occurs when the operation direction of the syringe pump is switched. This idling operation causes a shift (backlash) in the operation amount of the syringe pump, which causes an error in the dispensing amount.
  • Patent Document 1 does not fully consider such machine differences.
  • the present invention has been made in view of the above circumstances, and even if there is an individual difference in the backlash amount of the syringe pump, a dispensing device that can suppress an error in the dispensing amount due to this difference.
  • the purpose is to provide.
  • the dispensing device stores in advance the amount of operation of the drive device from when the drive device is instructed to reverse the operation direction of the syringe pump until it is actually reversed as the backlash amount, The operation amount of the drive device is corrected according to the backlash amount.
  • an optimum driving amount for removing backlash can be given to each individual syringe pump. Therefore, even if it is a case where the machine difference and the secular variation have arisen in the syringe pump, the error of the dispensing amount resulting from the individual difference can be suppressed.
  • FIG. 1 is a schematic configuration diagram of a syringe pump 105.
  • FIG. It is a schematic diagram explaining a general dispensing sequence.
  • 5 is a flowchart for explaining a procedure by which the dispensing apparatus 100 according to the first embodiment measures the backlash amount of the syringe pump 105.
  • It is a schematic diagram explaining the dispensing sequence in Embodiment 1.
  • It is a flowchart explaining the procedure in which the dispensing apparatus 100 which concerns on Embodiment 2 measures the backlash amount of the syringe pump 105.
  • FIG. It is a schematic diagram explaining the dispensing sequence in Embodiment 2.
  • the solenoid valve 107 When dispensing the liquid, the solenoid valve 107 is closed and the syringe pump 105 applies pressure to the inside of the dispensing nozzle 101, whereby the dispensing nozzle 101 sucks and discharges the sample / reagent. After dispensing, the electromagnetic valve 107 is opened from the base side and cleaning water is supplied.
  • the control unit 108 controls each unit of the dispensing device 100.
  • the storage unit 109 stores data such as driving conditions.
  • FIG. 2 is a schematic configuration diagram of the syringe pump 105.
  • a stepping motor 111, a syringe tube 112, and a photo interrupter 113 are fixed to the mechanism base 110.
  • a ball screw 114 is installed on the drive shaft of the stepping motor 111.
  • the plunger drive arm 115 is moved up and down.
  • the plunger 116 is attached to the plunger drive arm 115 and is inserted into the syringe tube 112.
  • the plunger drive arm 115 moves up and down
  • the plunger 116 moves up and down accordingly, and changes the internal volume of the syringe tube 112.
  • the detection plate 117 is installed on the plunger drive arm 115, and the origin position can be detected by checking whether or not the optical path of the photo interrupter 113 is shielded by the detection plate 117.
  • FIG. 3 is a schematic diagram for explaining a general dispensing sequence.
  • a process of dispensing the sample 119 contained in the sample container 118 to the reaction container 120 is illustrated.
  • the dispensing nozzle 101 is filled with system water 121.
  • the segment air 122 is sucked, and in FIG. 3C, a discharge operation for removing backlash is performed.
  • the driving pulse number corresponding to the amount finally sucked into the dispensing nozzle 101 is P air and the driving pulse number for removing backlash is P BL
  • P air + P BL is sucked in FIG.
  • PBL is discharged.
  • FIG. 3C PBL is discharged.
  • the dispensing nozzle 101 is inserted into the sample container 118, and the sample 119 is sucked in FIG. 3E.
  • the number of drive pulses corresponding to the dispensing amount of the sample and P S in FIG. 3 (e) sucking the P S + P BL.
  • FIG. 3 Move the dispensing nozzle 101 to the position of the cleaning tank 123 in (f), the sample 119 ejected P BL for removing backlash in the running water, that extra ejected as shown in FIG. 3 (g) is Wash away.
  • FIG. 3 (h) the dispensing nozzle 101 is moved to the reaction container 120, and the sample 119 held in the dispensing nozzle 101 in FIG. 3 (i) is discharged to the reaction container 120 together with the system water 121.
  • the number of drive pulses corresponding to the amount of system water 121 discharged together with the sample 119 is PSW
  • P S + P air + P SW is discharged in FIG.
  • FIG. 3 (j) the outer periphery of the dispensing nozzle 101 is washed with running water in the washing tank 123, and the inside is washed with system water.
  • the discharge operation for removing the backlash is performed, for example, in the gap existing in the fitting portion of the ball screw 114 and the plunger drive arm 115 in the syringe pump of FIG.
  • this is to eliminate the phenomenon of slipping when the suction and discharge are switched and the amount of movement of the plunger drive arm 115 deviating, that is, the influence of backlash.
  • a drive pulse larger than the maximum value of the assumed backlash amount is applied.
  • the backlash amount varies depending on the individual syringe pump 105 and varies depending on the wear of the parts, the amount of the sample 119 to be discharged as an extra amount in FIG. In the conventional dispensing sequence, an error caused by backlash is removed by dispensing the sample 119 after discharging the excess amount.
  • the present invention minimizes or completely eliminates the backlash removal, thereby achieving high-precision dispensing.
  • FIG. 4 is a flowchart for explaining a procedure by which the dispensing apparatus 100 according to the first embodiment measures the backlash amount of the syringe pump 105. Hereinafter, each step of FIG. 4 will be described.
  • Step S401 The control unit 108 returns the syringe pump 105 to the origin. Specifically, the plunger drive arm 115 is moved to the origin position. As a result, the detection signal of the photo interrupter 113 is turned on.
  • Steps S402 to S403 The control unit 108 causes the syringe pump 105 (that is, the stepping motor 111, the same applies hereinafter) to be sucked by one pulse (S402). The control unit 108 repeats step S402 until the detection signal of the photo interrupter 113 is turned off (S403).
  • the control unit 108 discharges the syringe pump 105 by one pulse (S405).
  • the control unit 108 checks whether or not the detection signal of the photo interrupter 113 is turned on (S406).
  • Control unit 108 if the detection signal is not ON counts up the P BL (S407).
  • the control unit 108 repeats steps S405 to S407 until the detection signal is turned ON.
  • Control unit 108 stores in the storage unit 109 the P BL as backlash of the syringe pump 105.
  • FIG. 5 is a schematic diagram for explaining a dispensing sequence according to the first embodiment.
  • the dispensing nozzle 101 is filled with system water 121.
  • the segmented air 122 is sucked at the drive pulse number P air + P BL
  • discharge for backlash removal is performed at the drive pulse number P BL . Since PBL is measured based on the backlash amount measuring method according to the present invention described with reference to FIG. 4, a predetermined amount of segmented air 122 is held in the dispensing nozzle 101 in FIG. No extra 122 is aspirated. Further, even when the discharge for backlash removal is performed in FIG. 5C, the amount of driving at that time is not excessive or insufficient, so that the liquid and air in the dispensing nozzle 101 do not move.
  • the dispensing nozzle 101 is inserted into the sample container 118, and in FIG. 5E, the sample 119 is sucked with the drive pulse number P S + P BL , and in FIG. After moving the dispensing nozzle 101, PBL is discharged in order to remove backlash in FIG. Also in FIG. 5E, a predetermined amount of sample is held by the dispensing nozzle 101, and the sample is not sucked excessively. Even when the backlash removal discharge is performed in FIG. 5G, the liquid or air does not move. In FIG. 5 (h), the dispensing nozzle 101 is moved to the reaction vessel 120, and the sample 119 held in the dispensing nozzle 101 in FIG.
  • FIG. 5 (i) is driven with the number of drive pulses P S + P air + P SW together with the system water 121. It discharges to the reaction container 120, and in FIG.5 (j), the dispensing nozzle 101 is wash
  • FIG. 5 (j) is driven with the number of drive pulses P S + P air + P SW together with the system water 121. It discharges to the reaction container 120, and in FIG.5 (j), the dispensing nozzle 101 is wash
  • the dispensing apparatus 100 instructs the stepping motor 111 to reverse the operation direction of the syringe pump 105 and then the photo interrupter 113 indicates that the syringe pump 105 has started to move in the opposite direction. Until the detection, the number of pulses output to the stepping motor 111 is stored as a backlash amount. The dispensing apparatus 100 corrects the number of pulses output to the stepping motor 111 when the operation direction of the syringe pump 105 is reversed using the backlash amount.
  • the sample 119 can be dispensed with high accuracy.
  • FIG. 6 is a flowchart for explaining a procedure by which the dispensing apparatus 100 according to the second embodiment measures the backlash amount of the syringe pump 105. Hereinafter, each step of FIG. 6 will be described.
  • Step S601 The control unit 108 sucks the syringe pump 105 until the plunger 116 reaches the measurement start position. At this time, the backlash in the suction direction of the syringe pump 105 needs to be reliably removed. If the suction operation amount is less than the assumed maximum backlash amount, perform the discharge operation immediately before the start of suction so that the suction operation amount exceeds the maximum backlash amount, and then exceed the maximum backlash amount. To suck.
  • the control unit 108 opens the electromagnetic valve 107, fills the dispensing nozzle 101 with the system water 121, and closes the electromagnetic valve 107 (S602).
  • the user operates the dispensing mechanism so that the tip of the dispensing nozzle 101 is closed (S603).
  • the dispensing nozzle 101 may be lowered to the bottom surface of the dispensing apparatus 100, or (b) a member that closes the tip of the dispensing nozzle 101 is installed in advance within the movable range of the dispensing mechanism. Alternatively, the dispensing nozzle 101 may be moved to that position.
  • a soft material such as rubber is suitable.
  • the control unit 108 discharges the syringe pump 105 by one pulse (S605).
  • the control unit 108 determines whether or not the pressure detected by the pressure sensor 104 has increased (S606).
  • Control unit 108 if not detected pressure increase increments the P BL (S607).
  • Control unit 108 repeats steps S605 to S607 until an increase in pressure is detected.
  • Control unit 108 stores in the storage unit 109 the P BL as backlash of the syringe pump 105.
  • FIG. 7 is a schematic diagram for explaining a dispensing sequence in the second embodiment.
  • the conventional dispensing sequence is followed to remove backlash and switch between suction and discharge.
  • the backlash amount can be measured accurately, it is considered sufficient to operate the stepping motor 111 in consideration of the backlash amount when switching between suction and discharge.
  • FIG. 7 illustrates a modification of the dispensing sequence described in FIG.
  • the dispensing nozzle 101 is filled with system water 121.
  • the segment air 122 is sucked by the number of drive pulses P air + P BL . It is the same as in the first embodiment that the segment air 122 is not sucked excessively.
  • Figure 7 Insert the sample vessel 118 the dispensing nozzle 101 (c), the aspirating the sample 119 in the driving pulse number P S in FIG. 7 (d). Since the suction operation is performed immediately before FIG. 7D, backlash removal is not necessary in FIG. 7D.
  • the dispensing nozzle 101 is moved to the reaction container 120, and the sample 119 held in the dispensing nozzle 101 in FIG. 7 (f) is driven with the system water 121 and the number of drive pulses P S + P air + P SW + P It discharges to the reaction container 120 by BL . Since the sample suction operation is performed immediately before FIG. 7F, the backlash removal is added to the discharge amount. In FIG. 7G, the dispensing nozzle 101 is washed with running water and system water in the washing tank 123.
  • the dispensing apparatus 100 uses the pressure sensor 104 instead of the photo interrupter 113 to measure the backlash amount. Thereby, even if the plunger 116 is not necessarily in a position that can be detected by the photo interrupter 113, the same effect as in the first embodiment can be exhibited.
  • the present invention is not limited to the embodiments described above, and includes various modifications.
  • the above-described embodiment has been described in detail for easy understanding of the present invention, and is not necessarily limited to the one having all the configurations described. Further, a part of the configuration of an embodiment can be replaced with the configuration of another embodiment, and the configuration of another embodiment can be added to the configuration of an embodiment. In addition, it is possible to add, delete, and replace other configurations for a part of the configuration of each embodiment.
  • the drive mechanism using the ball screw 114 is exemplified, but a similar drive mechanism can be realized by using a gear or a timing belt.
  • a similar drive mechanism can be realized by using a gear or a timing belt.
  • the use of the stepping motor 111 has been described in the above embodiment, even when another motor (for example, a servo motor) is used, the back amount is measured by measuring the drive amount of the motor as in the above embodiment. Rush amount can be measured.
  • the photo interrupter 113 is used as a sensor for detecting the position of the plunger 116, but the position of the plunger 116 may be detected by other sensors. For example, (a) a contact sensor or proximity sensor using magnetism or electricity, (b) a change in distance between the plunger drive arm 115 and the plunger 116 is measured by a displacement sensor, and (c) a plunger drive arm 115 by an image sensor. Alternatively, the movement of the liquid in the plunger 116 or the pipe 106 may be measured.
  • the number of divisions of the stepping motor 111 is increased by microstep driving the stepping motor 111, and the measurement result is converted to the original number of divisions. Good.
  • the backlash amount when the backlash amount differs for each position where the operation direction of the plunger 116 starts to reverse, the backlash amount is set for each position of the plunger 116 by, for example, arranging the photo interrupter 113 at a plurality of positions. You may measure. In this case, the backlash amount is stored in the storage unit 109 for each position where the operation direction of the plunger 116 starts to reverse, and the backlash is removed using this.
  • control unit 108 may issue a warning to the user. .
  • the following may be considered as the timing for executing the flowchart of FIG. 4 or FIG. 6: (a) performed before shipping the dispensing apparatus 100; (C) Perform every predetermined period. Furthermore, (a) may be performed for each model of the dispensing apparatus 100.
  • Dispensing device 101 Dispensing nozzle 102: Arm 103: Shaft 104: Pressure sensor 105: Syringe pump 106: Piping 107: Electromagnetic valve 108: Control unit 109: Storage unit 111: Stepping motor 113: Photointerrupter 116: Plunger

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne un dispositif de distribution avec lequel, même s'il existe des différences individuelles dans les quantités de jeu de guide-seringue, il est possible de supprimer des erreurs dans la quantité distribuée dues auxdites différences. Un dispositif de distribution selon la présente invention stocke à l'avance, en tant que quantité de jeu, une quantité de fonctionnement de dispositif d'entraînement mesurée à partir du moment où le dispositif d'entraînement reçoit l'instruction d'inverser la direction de fonctionnement d'un guide-seringue jusqu'à ce que la direction de fonctionnement ait réellement été inversée, et corrige la quantité de fonctionnement du dispositif d'entraînement en fonction de ladite quantité de jeu.
PCT/JP2019/008228 2018-03-29 2019-03-01 Dispositif de distribution et procédé de distribution Ceased WO2019187996A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018063655A JP2019174318A (ja) 2018-03-29 2018-03-29 分注装置、分注方法
JP2018-063655 2018-03-29

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WO2019187996A1 true WO2019187996A1 (fr) 2019-10-03

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2024157846A1 (fr) * 2023-01-23 2024-08-02

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0429033A (ja) * 1990-05-25 1992-01-31 Sanyo Electric Co Ltd 血漿滴下制御装置
JPH07333231A (ja) * 1994-06-10 1995-12-22 Aloka Co Ltd 自動分注装置
JP2010284015A (ja) * 2009-06-05 2010-12-16 Mycom Inc ステッピングモータ駆動装置及びコントローラ
JP2012159315A (ja) * 2011-01-31 2012-08-23 Hitachi High-Technologies Corp 分析装置
JP2015516583A (ja) * 2012-05-15 2015-06-11 ウェルスタット ダイアグノスティクス，エルエルシー 器具およびカートリッジを含む臨床診断システム

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0429033A (ja) * 1990-05-25 1992-01-31 Sanyo Electric Co Ltd 血漿滴下制御装置
JPH07333231A (ja) * 1994-06-10 1995-12-22 Aloka Co Ltd 自動分注装置
JP2010284015A (ja) * 2009-06-05 2010-12-16 Mycom Inc ステッピングモータ駆動装置及びコントローラ
JP2012159315A (ja) * 2011-01-31 2012-08-23 Hitachi High-Technologies Corp 分析装置
JP2015516583A (ja) * 2012-05-15 2015-06-11 ウェルスタット ダイアグノスティクス，エルエルシー 器具およびカートリッジを含む臨床診断システム

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