[go: up one dir, main page]

WO2019165567A1 - Système et procédé de biorémédiation d'eaux contaminées - Google Patents

Système et procédé de biorémédiation d'eaux contaminées Download PDF

Info

Publication number
WO2019165567A1
WO2019165567A1 PCT/CL2018/050056 CL2018050056W WO2019165567A1 WO 2019165567 A1 WO2019165567 A1 WO 2019165567A1 CL 2018050056 W CL2018050056 W CL 2018050056W WO 2019165567 A1 WO2019165567 A1 WO 2019165567A1
Authority
WO
WIPO (PCT)
Prior art keywords
bioreactor
settling tank
microorganisms
water
effluent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CL2018/050056
Other languages
English (en)
Spanish (es)
Inventor
Marcela Eugenia MUÑOZ AGUIRRE
Rory Laten TIBBALS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ard Solutions Ltda
Original Assignee
Ard Solutions Ltda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ard Solutions Ltda filed Critical Ard Solutions Ltda
Publication of WO2019165567A1 publication Critical patent/WO2019165567A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/58—Treatment of water, waste water, or sewage by removing specified dissolved compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/58—Treatment of water, waste water, or sewage by removing specified dissolved compounds
    • C02F1/62—Heavy metal compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/70—Treatment of water, waste water, or sewage by reduction
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/06—Aerobic processes using submerged filters
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • the invention relates to a bioreactor system and the method of application of said system that allows bioremediation of contaminated water, such as natural effluents contaminated by industrial activity.
  • Passive systems for the remediation of contaminated water are known in the art, among those existing for surface flows are: artificial wetlands, anoxic drains, organic rafts and alkaline production systems; for underground flows the permeable reactive barriers (PRB, Permeable Reactive Barriers), and for mining lakes the anaerobic bioprocesses.
  • PRB permeable reactive barriers
  • the main objective of these systems is the suppression of acidity, the precipitation of heavy metals and the elimination of polluting substances. A brief description of these passive systems is given below.
  • Aerobic wetlands In artificial aerobic wetlands it is intended to reproduce the phenomena and processes of natural wetlands (swamps, marshes, peat bogs, etc.), creating an environment conducive to the development of certain plants (Tipha, Equisetum, reed, reeds, etc. .), communities of organisms (algae, protozoa and bacteria) and mosses (Sphagnum), which participate in water purification.
  • An aerobic system usually consists of one or several connected cells through which water circulates slowly by gravity, establishing a horizontal horizontal flow.
  • systems are designed that include waterfalls, meandering beds and rafts of large areas with shallow depths where hydrophilic plant frames that cover about 40% of the wetland surface are implanted. Emerging plants used in wetlands can transfer up to about 45 g 0 2 / m 2 / day through their roots and create an aerobic zone in the wetland substrate where metal oxidation and precipitation occurs.
  • Anaerobic wetlands In this type of wetland mine water flows by gravity and the increase in pH to levels close to neutral is due to the alkalinity of the bicarbonates generated in the system from anaerobic sulfate reduction and dissolution of limestone (CaCC> 3 ). To prevent aerobic processes that trigger the generation of metallic acidity through the hydrolysis of some metals, the pre-treatment of acidic water with limestone in atmospheric conditions is used.
  • Anoxic limestone drainage This system consists of a ditch filled with limestone gravels or other limestone material sealed to the ceiling by a layer of clay soil and an impermeable geomembrane to maintain anoxic conditions, thereby increasing the partial pressure of the C0 2 to maximize the dissolution of limestone. Acid mine water is circulated inside the ditch causing limestone to dissolve, which creates alkalinity and raises the pH of the water.
  • Channel or limestone toxic drainage It is a channel whose bed is filled with limestone through which the water to be treated flows, whose objective is to increase the pH and alkalinity to decrease acidity.
  • the high oxygen content produces oxidation and hydrolysis of Fe and dissolved metals, which precipitate as oxyhydroxides.
  • PRB Permeable reactive barriers
  • Its construction consists of making a trench transverse to the flow, which is filled with various types of reactive materials.
  • the purifying processes inside the barrier are the bacterial reduction of sulfates, the retention of metals precipitating as sulphides, and the increase in pH mainly by dissolving the limestone.
  • Passive acid water treatments involve treatment in gravitational flow systems (without pumping) that contain natural materials (fertilizer, limestone, etc.). Passive systems improve water quality through biogeochemical reactions without the use of synthetic reagents and without the application of external energy, which results in a cheaper option to decontaminate these waters. In addition, it only requires sporadic (although regular) maintenance.
  • Traditional passive treatment systems such as oxic limestone drains (OLD), limestone drainage (anoxic limestone drains, ALD), alkalinity generating and reducing systems (reducing and alkalinity producing Systems, RAPS), are prone to fill (get stuck) and passivate (lose reactivity) when used to treat water with high acidity and metallic charge, typical characteristics of mine acid waters.
  • patent EP301924B1 discloses a wastewater treatment process to remove phosphorus and optionally nitrogen, from these wastewater , of the type of activated sludge or biofilter, which operates in aerobic conditions and subsequently in anaerobic conditions in a treatment pond, with the use of reducing bacteria, agitation means to maintain dissolved oxygen at a concentration of 1 to 3.2 mg / l and have Steel contacts immersed in the pond to use electrochemical corrosion of iron.
  • the method of this patent requires a lot of energy to maintain the aerobic conditions of the process.
  • JP2008194610A provides a treatment method that allows maintaining the concentration and activity of the sulfur oxidizing bacteria without additional supply of substrates so that these bacteria perform a stable treatment, when the wastewater they contain Sulfur-based COD components are treated biologically with sulfur oxidizing bacteria.
  • the process comprises a first aerobic oxidation stage, with oxygen supply, followed by a reduction stage, with constant agitation. This process also requires a high energy supply in your application.
  • the present invention relates to the treatment of contaminated water.
  • the present invention relates to a system that includes a bioreactor and the method of applying said system for bioremediation of contaminated water, such as for example acidic water.
  • the main objective of this technology is the remediation of contaminated water from an effluent. Therefore, once the effluent that will be remedied has been defined, analysis should be done to determine the existing levels of the various pollutants. A bioreactor is then constructed to remedy the effluent under analysis in situ.
  • a bioremediation system and method comprising a settling tank connected to a bioreactor comprising a consortium of microorganisms with sulfo reductive characteristics that perform biological remediation.
  • the innovative system and method of the invention consists of a reductive system that performs two functions in two separate compartments: the first: Precipitation of metal sulphides, carbonates, oxides, and elemental sulfur and the second: Biological generation of sulphides and alkalinity.
  • Figure 1 Schematic drawing of one of the modalities of the semi-passive system of the invention.
  • FIG. 1 Schematic drawing of another embodiment of the semi-passive system of the invention, which includes a mixing chamber (11).
  • FIG. 1 Schematic drawing of another embodiment of the system of the invention, with 2 separate reactors.
  • FIG. 4 Schematic drawing of another embodiment of the system of the invention, with 4 separate reactors, a settling tank and 3 bioreactors in series, with a nutrient delivery.
  • FIG. 5 Schematic drawing of another embodiment of the system of the invention, with 4 separate reactors, a settling tank and 3 bioreactors in series, where the delivery of nutrients is through a plurality of serial connections of the 3 bioreactors.
  • Figure 6. Schematic drawing of another embodiment of the system of the invention, with 5 separate reactors, a settling tank and 4 bioreactors in series, with serial nutrient feed, with a sand filter (30), a contaminated water tank (20) and a nutrient pond (40).
  • the invention relates to a system and method for the bioremediation of contaminated water, such as acidic waters from natural effluents contaminated by industrial activity, such as mining activity.
  • contaminated water such as acidic waters from natural effluents contaminated by industrial activity, such as mining activity.
  • the system and method of the invention make it possible to purify contaminated water at a low cost.
  • the present invention consists of a system comprising two separate compartments, one for the biological purification of water by means of microorganisms, especially consortium of microorganisms with sulforeductive characteristics, and another for the precipitation of solids.
  • the system is semi-passive and energy dependent. It can have a single large separate pool in two compartments or have several separate ponds, which can be a decanter and one or more bioreactors in series.
  • the system is semi-passive and comprises a settling tank, which is connected by overflow to the bioreactor, as illustrated in Figures 1 or 2.
  • the contaminated effluent reaches a settling tank, where in a first chamber it is Mix with recirculated treated water.
  • the water in the settling pond is overflowed into a bioreactor, which comprises aggregates colonized by microorganisms and receives nutrients from a nutrient supply pond.
  • energy is provided by means of a solar panel or other autonomous system available in the art, and if possible, network power can be used.
  • the treated water flows out of the system, specifically from the settling tank.
  • the settling tank of the system of the invention comprises a sludge outlet, and if said outlet is not present, these can be extracted by means of extraction available in the art, such as a truck with suction means, mechanical shovels or Other means of extraction.
  • the system comprises a settling tank (1), which is connected by pipes (7) and pumps to the bioreactor (2), as illustrated in Figure 3.
  • the contaminated effluent reaches the settling tank, where in a first chamber it is mixed with treated recirculated water. Water from the settling pond is pumped to the bioreactor, which comprises aggregates colonized by microorganisms and receives nutrients from a nutrient pond.
  • the treated water flows out of the system, specifically from the settling tank.
  • the settling tank of the system of the invention comprises a sludge outlet.
  • bioreactors In an optional embodiment there are several bioreactors connected in series, as illustrated in Figure 4.
  • the process of the invention which is carried out in any of the aforementioned systems, performs two functions in two separate compartments: 1. Biological generation of sulphides and alkalinity, in the bioreactor and 2. Precipitation of metal sulphides, carbonates, oxides, and sulfur elemental, and neutralization of sulfuric acid in the settling pond.
  • the size of the settling pond is designed to allow the precipitation of the metals of the solution to be treated, the decantation of the precipitated sludge and the storage of sludge (precipitated metals).
  • the microorganisms, located in the bioreactor are mainly bacteria, archaea, fungi or protozoa, especially bacteria and sulfo reducing archaea.
  • a source of inorganic or organic nutrients can be supplied that are easily pumped in small volumes to the bioreactor.
  • a solution from the bioreactor whose product of the activity of the microorganisms has an alkaline pH (about 7), sulphides, and bicarbonate concentrations, is recirculated and mixed with the inlet solution (solution to be treated). This neutralizes acidity and causes precipitation of sulphides, oxides and carbonated species. Recirculation is carried out by means of a pump.
  • the energy source of this pump can be a solar panel, like other available energy sources, including grid power.
  • the microorganisms contained in the bioreactor colonize the aggregates present, the which are chosen from gravel, gravel or limestone, among others, and will use nutrients as a source of food and sulfate contained in the solution from the settling pond as a source of oxygen.
  • Sulfo reducing bacteria will produce sulfide, additional alkalinity, and bicarbonate, which in turn allows the neutralization and precipitation of metals in the settling pond. Since the two compartments have a fixed volume, the treated decontaminated water will leave the system by overflow to maintain the level in the system. That is to say, the flow of contaminated water entering the system is proportional flow of treated water leaving (see figure 1).
  • This system requires minimal operation, so visits to the system site for operational and preventive maintenance purposes can be carried out weekly or even monthly depending on the criticality or degree of contamination of the water, that is, concentration of metals in the effluent of entry. All necessary functions can be performed in a single work day: 1. Preventive maintenance and equipment calibration, to ensure continuous operation. 2. Sludge disposal. 3. Address unforeseen equipment operations. In particular, sludge removal is done even more spacedly, it can be every 4, 6, 8, 10 or 12 months.
  • a configuration of a bioreactor of the invention in the semi-passive system mode shows the system comprising two communicated reactors, through a overflow and a recirculation means, wherein the first reactor is a settling tank (1) comprising: a. a mixing chamber (1 1), which comprises a contaminated water inlet (3), a recirculated effluent connection pipe (4), and a mixture outlet (14); and b. a treated water outlet (6); wherein the second reactor is a bioreactor (2) comprising: a. a bioreactor inlet (7) that receives the outflow of the settling tank (1), which corresponds to an outlet of the settling tank; b.
  • a nutrient input (5) for microorganisms where nutrients are supplied in a controlled manner, with a pumping system (13); c. a plurality of aggregate particles (10), where the microorganisms can be anchored; and d. a recirculating pump (12) that communicates operatively with the mixing chamber (11).
  • the settling tank (1) is overflowed to the bioreactor (2) and is separated by a barrier (9).
  • the contaminated effluent reaches the settling tank through the inlet (3), where the inlet is mixed with the recirculated effluent (4) in a mixing chamber (11).
  • the water passes overflow (7) to the bioreactor (2) that comprises aggregate particles (10) colonized by microorganisms and receives nutrients through the entrance (5) from a nutrient pond.
  • the treated water flows out of the reactor through the outlet (6). And optionally there is the outlet for decanted sludge (8).
  • the settling tank (1) is connected by pipes (7) with at least one pump to the bioreactor (2).
  • the contaminated effluent reaches the settling tank through the entrance (3), at the entrance it is mixed with recirculated effluent (4) in a mixing chamber (11).
  • the water passes through pipes with at least one pump (7) to the bioreactor (2) that comprises aggregate particles (10) colonized by microorganisms and receives nutrients through the entrance (5) from a nutrient pond .
  • the treated water flows out of the reactor through the outlet (6).
  • the outlet for decanted sludge there is the outlet for decanted sludge (8).
  • the stages it comprises are: first entering the contaminated water into the settling tank, where it is mixed with a flow of recirculation from the bioreactor, which generates the precipitation of the metals present in the contaminated water and then conducts this water with sulfate concentration towards the bioreactor with microorganisms, especially a consortium of microorganisms with sulfo reductive characteristics that reduce sulfate by increasing the alkalinity of the medium and recirculating part of the bioreactor water, as already indicated towards the settling pond to be mixed with the contaminated effluent and favor the precipitation of the metals to obtain the treated water that leaves the system by overflow.
  • the system additionally comprises adding nutrients for the microorganisms contained in the bioreactor, where the inlet and outlet flow of the system is proportional, generally 1: 1 and the recirculation flow is from 1: 1 to 1: 50.
  • the residence time within the system is between 4 hours to 10 days.
  • the nutrients to be added depend on the microorganisms chosen. These can be bacteria, archaea, fungi, protozoa or others, Sulfo reducing bacteria are especially chosen, which can be of the genera Acidithiobacillus, Sulfobacillus, Pseudomonas, Acidiphilium, Leptospirillum, or others and the archaea are chosen among the genera: Acidianus , Ferroplasma, Metallosphaera, Sulfolobus or others. . But in general they must incorporate a source of carbon and inorganic essential nutrients such as phosphate, nitrogen, potassium.
  • ethyl alcohol and NPK fertilizer are incorporated separately, other options are methanol, propanol, butanol, and even any available organic compound, such as sugar and even milk.
  • concentration of nutrients must be established in relation to the effluent to be treated.
  • the system could be used to treat nitrate waters, where metals and sulfate are present.
  • the invention could be used to precipitate soluble metals in neutral water streams such as arsenic or selenium.
  • the bioreactor and the settling tank would normally be constructed in an underground pond system (underground, buried). In another application, the bioreactor and the settling tank could be arranged on the surface. In another application, the bioreactor and the settling pond could be built in mine tunnels.
  • the bioreactor can be constructed and / or coated with HDPE, Hypalon, pvc, asphalt, concrete, clay, for example.
  • the settling tank may be constructed and / or coated with steel, polyethylene, HDPE, stainless steel and fiberglass.
  • the decanter and / or bioreactor may be covered to ensure anoxic conditions, prevent animals or even people from drinking from the reactors and protect the system from falling animals or other occasional contaminants.
  • One option is the coating with an HDPE cover, so that the cover is held above the reactor, a layer of can be included. HDPE balls, where these balls are empty and filled with air, and on them the HDPE cover is placed. Similarly, any other coating system available in the art can be used.
  • the system may comprise a sand filter at the outlet of the decanter, as a final treatment, in order to retain very fine metals that do not precipitate in the decanter.
  • the invention corresponds to an industrial process, which comprises a system and method for remediation with bacteria from contaminated water. This process It can be done through a semi-passive system, which has the following comparative advantages:
  • the operational equipment is very few and of very low energy requirement.
  • the required electrical energy can be provided by solar collectors or small wind station, or other system available in the art.
  • the system is remotely controllable.
  • the system reduces energy consumption by being semi-passive and using anaerobic bacteria.
  • the present technology discloses a system for the purification of contaminated water comprising at least two communicated reactors and a recirculation medium
  • the first reactor is at least one settling tank (1 ) comprising: a. an inlet (3) of contaminated water and a flow of recirculated water, coming from a connecting pipe of the recirculated effluent (4); b. a treated water outlet (6);
  • the second reactor is at least one bioreactor (2), which can be closed, to prevent the entry of air, comprises: a. a bioreactor inlet (7) that receives the outflow from the upper level of the fluid in the settling tank (1); b.
  • a nutrient input (5) to feed the microorganisms c. a plurality of aggregate particles (10), which are housed in the bioreactor (2), where the microorganisms can be anchored; and d. a recirculating pump (12) that communicates operatively with the connection pipe of the recirculated effluent (4), so that said effluent enters the settling tank (1).
  • the settling tank (1) also comprises: a mixing chamber (1 1), which mixes the fluids from the inlet (3) of contaminated water and the connection pipe of the recirculated effluent (4), and an outlet of the mixture (14); which delivers the mixed fluids to said settling tank (1).
  • the settling tank (1) also comprises a sludge outlet (8).
  • the system additionally comprises a pump (13) to deliver the nutrients (5) to the bioreactor (2) from a nutrient pond, when the delivery of nutrients by gravity is not sufficient.
  • the aggregate particles (10), are gravel or gravel or limestone or mixture of both or any organic or inorganic material that has permeability, suitable to be colonized by microorganisms and with a diameter of up to 500 mm.
  • the outlet for the decanted sludge (8) is at the bottom of the settling tank (1).
  • the outlet of the settling tank towards the bioreactor (7) can be carried out by overflow or by pipes operated by pumps.
  • the system is powered by solar or wind energy panels, or any means of autonomous energy or mixture of said energy sources.
  • the second reactor is a plurality of bioreactors (2.0; 2.1; 2.2), which can be connected in series or in parallel or in series and parallel.
  • the microorganisms are chosen among bacteria, archaea, fungi, protozoa and especially among sulforeductive bacteria of the genera Acidithiobacillus, Sulfobacillus, Pseudomonas, Acidiphilium, Leptospirillum, or others, and sulforeducting archaees of the genera: Acidianus, Ferroplasma, Metaphabus other .
  • Nutrients are chosen from methanol, ethanol, butanol, acetate, sugar, malaza, dairy products, such as: cheese whey, milk and NPK fertilizers, among others.
  • a barrier (9) When the settling tank (1) and the bioreactor (2) are constructed in the same unit, said ponds are separated by a barrier (9). It can be constructed and / or coated with any suitable material available in the art. Non-limiting examples of suitable materials are: HDPE (High Density Polyethylene-High Density Polyethylene) or Hypalon, PVC, asphalt, concrete, clay, for example.
  • the at least two communicated reactors are constructed and / or coated with: steel, polyethylene, HDPE, (High Density Polyethylene - high density polyethylene) or Hypalon or PVC or concrete or stainless steel, or fiberglass, among others.
  • the settling tank (1) is closed or covered, so that no contaminants, foreign agents and / or air enter.
  • a method for the purification of contaminated water comprising: a. decant metals from contaminated water in a settling pond (1), b. treat water in a bioreactor with sulfo reducing microorganisms, and c.
  • the first reactor is a settling tank (1) comprising: an inlet (3) of contaminated water and a connection pipe of the recirculated effluent (4); a treated water outlet (6); wherein the second reactor is at least one bioreactor (2) comprises: a bioreactor inlet (7) that receives the outflow from the upper level of the fluid that is in the settling tank (1); a nutrient input (5) for microorganisms; a plurality of aggregate particles (10), which are housed in the bioreactor (2), where the bacteria can be anchored; and a recirculating pump (12) that communicates operatively with the connection pipe of the recirculated effluent (4), so that said effluent enters the settling tank (1).
  • nutrients can be added to the bacteria contained in the bioreactor (2) and recirculate fluid from the bioreactor to the settling tank (1).
  • the input flow of the system is proportional to the output flow, for example, at a rate between 2: 1 to 1: 2, especially 1: 1 and the recirculation flow is 1: 1 to 1: 50, where The residence time within the system is between 1 hour to 15 days.
  • Microorganisms are found colonizing gravel or gravel where aggregate particles have a diameter of at most 500 mm. These are chosen among Acidithiobacillus, Sulfobacillus, Pseudomonas, Acidiphilium, Leptospirillum, among others.
  • Nutrients are chosen from methanol, ethanol, butanol, acetate, sugar, malaza, dairy products, such as: cheese whey, NPK fertilizer milk or any organic nutrient.
  • NPK Fertilizer 16PPM N; 5 PPM P and 2 PPM K.
  • HDPE balls with HDPE cover where these balls are empty and filled with air. They are located under the HDPE cover to keep it floating. This is to prevent animals, birds and humans from entering or drinking water. Also to prevent evaporation and loss of superfluous and air pollution with H2S.
  • Pond size 20m x 15m x 3m (LxWxH). Pond volume: 441 m 3 Current residence time: 133 hours without decanted sludge.
  • EXAMPLE 2 4 bioreactors in series and a settling tank were used, as shown in Figure 6. These results were obtained by using 5 tanks of 1200 liters (1; 2.0; 2.1; 2.2; 2.3) and 3 tanks of 200 liters (20, 30, 40). One of the 1200 liter ponds is the metal decanting tank (1). The other 4 tanks of 1200 liters are the bioreactors in series (2.0; 2.1; 2.2 and 2.3). Each bioreactor is full of gravel and water (500 liters each). The gravel fulfills the function of being a support for microorganisms.
  • Water from the bioreactor (2.3) contains many sulphides due to bacterial sulfate reduction that occurs in bioreactors (2.0; 2.1; 2.2; 2.3). When this water was combined with sulphides, with the contaminated water containing many metals, the precipitation of these in the form of sludge occurred and they were deposited at the bottom of the decanter (1).
  • PH levels, oxidation / reduction potential, TDS and conductivity were measured daily throughout the experiment.
  • the sampling points are below the decanter (1), below the bioreactor (2.0), below the bioreactor (2.1), below the bioreactor (2.2), below the bioreactor (2.3), in the decanter supernatant (1) above, in the bioreactor supernatant (2.3) and in the water after passing through the sand filter (30), that is, the clean water (filter).
  • the system was running 34 days without contaminated water, only with nutrients. Enough time for the microorganisms to stabilize, increase the biomass to an optimal figure and achieve the necessary alkalinity to subsequently precipitate metals with an oxygen source such as sulfate under anoxic conditions.
  • the output pH is absolutely neutral, and there is a substantial reduction of the sulfates present, as well as of the heavy metals Aluminum, Cobalt, Copper Copper, Manganese, Zinc, Arsenic, Beryllium, Cadmium , Chrome, Lithium (Citrus), Molybdenum, Nickel, Silver and Vanadium.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

La présente invention concerne un système pour la purification d'eaux contaminées qui comprend au moins deux réacteurs reliés et un moyen de recirculation, le premier réacteur est au moins un bassin de décantation (1) qui comprend: a. une entrée (3) d'eaux contaminées et un flux d'eau recirculée provenant d'une conduite de raccordement de l'effluent recirculé (4); b. une sortie d'eau traitée (6); lequel second réacteur est au moins un bioréacteur (7) qui reçoit le flux de sortie depuis le niveau supérieur du fluide qui se trouve dans le bassin de décantation (1); f. une entrée de nutriments (5) pour les microorganismes; g. une pluralité de particules d'agrégats (10), de celles qui se logent dans le bioréacteur (2), où peuvent se fixer les microorganismes; et h. une pompe de recirculation (12) qui est reliée fonctionnellement à la conduite de raccordement de l'effluent recirculé (4) pour que ledit effluent entre dans le bassin de décantation (1) et un procédé associé au système 15 pour purifier les eaux contaminées.
PCT/CL2018/050056 2018-02-28 2018-07-20 Système et procédé de biorémédiation d'eaux contaminées Ceased WO2019165567A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CLN°550-2018 2018-02-28
CL2018000550A CL2018000550A1 (es) 2018-02-28 2018-02-28 Sistema y método de biorremedación de aguas contaminadas

Publications (1)

Publication Number Publication Date
WO2019165567A1 true WO2019165567A1 (fr) 2019-09-06

Family

ID=63046485

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CL2018/050056 Ceased WO2019165567A1 (fr) 2018-02-28 2018-07-20 Système et procédé de biorémédiation d'eaux contaminées

Country Status (2)

Country Link
CL (1) CL2018000550A1 (fr)
WO (1) WO2019165567A1 (fr)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0436254A1 (fr) * 1990-01-05 1991-07-10 Shell Internationale Researchmaatschappij B.V. Traitement de courants résiduaires aqueux
US5587079A (en) * 1995-04-21 1996-12-24 Rowley; Michael V. Process for treating solutions containing sulfate and metal ions.
US5976372A (en) * 1995-01-14 1999-11-02 Vesterager; Niels Ole Method of treating a biomass in order to remove heavy metals with hydrogen sulphide
US6197196B1 (en) * 1998-10-15 2001-03-06 Water Research Commission Treatment of water
US6387669B1 (en) * 1998-12-21 2002-05-14 Battelle Memorial Institute Methods for producing hydrogen (BI) sulfide and/or removing metals
US20070278150A1 (en) * 2006-06-06 2007-12-06 Lupton Francis S System and methods for biological selenium removal from water
ES2338988T3 (es) * 2000-10-25 2010-05-14 The Regents Of The University Of California Recuperacion de agua y de un concentrado de salmuera utilizable a partir de aguas residuales domesticas.
US20100176055A1 (en) * 2006-08-16 2010-07-15 Michael Riebensahm Method for removing sulphate and heavy metals from waste water
AU2007202864B8 (en) * 2006-06-27 2011-07-14 Universidad Catolica Del Norte Biotechnological process for the treatment of As-containing hydroxide sludge resulting from potable water treatment processes which use FeC13 as a coagulant, through the action of sulfate reducing bacteria
US20120003057A1 (en) * 2010-07-02 2012-01-05 Leyba Frank L Wrenchable drill bit

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0436254A1 (fr) * 1990-01-05 1991-07-10 Shell Internationale Researchmaatschappij B.V. Traitement de courants résiduaires aqueux
US5976372A (en) * 1995-01-14 1999-11-02 Vesterager; Niels Ole Method of treating a biomass in order to remove heavy metals with hydrogen sulphide
US5587079A (en) * 1995-04-21 1996-12-24 Rowley; Michael V. Process for treating solutions containing sulfate and metal ions.
US6197196B1 (en) * 1998-10-15 2001-03-06 Water Research Commission Treatment of water
US6387669B1 (en) * 1998-12-21 2002-05-14 Battelle Memorial Institute Methods for producing hydrogen (BI) sulfide and/or removing metals
ES2338988T3 (es) * 2000-10-25 2010-05-14 The Regents Of The University Of California Recuperacion de agua y de un concentrado de salmuera utilizable a partir de aguas residuales domesticas.
US20070278150A1 (en) * 2006-06-06 2007-12-06 Lupton Francis S System and methods for biological selenium removal from water
AU2007202864B8 (en) * 2006-06-27 2011-07-14 Universidad Catolica Del Norte Biotechnological process for the treatment of As-containing hydroxide sludge resulting from potable water treatment processes which use FeC13 as a coagulant, through the action of sulfate reducing bacteria
US20100176055A1 (en) * 2006-08-16 2010-07-15 Michael Riebensahm Method for removing sulphate and heavy metals from waste water
US20120003057A1 (en) * 2010-07-02 2012-01-05 Leyba Frank L Wrenchable drill bit

Also Published As

Publication number Publication date
CL2018000550A1 (es) 2018-07-06

Similar Documents

Publication Publication Date Title
US5893975A (en) Enhanced subsurface flow constructed wetland
CN100494091C (zh) 一种修复矿山生态环境的方法
ES2708764T3 (es) Saneamiento del drenaje de mina usando un sustrato alcalino disperso en carbonato de bario
CN211871728U (zh) 一种煤矿酸性废水综合处理系统
US10597318B2 (en) Bioreactor for sulfate reduction
CN110407414A (zh) 一种酸性矿山废水处理方法
CN102007076B (zh) 用于处理污水的植物系统
Sheoran Management of acidic mine waste water by constructed wetland treatment systems: a bench scale study
SØNDERGAARD et al. 10• Chemical treatment of water and sediments with special reference to lakes
KR101070477B1 (ko) 준자연정화식 광산배수 처리장치 및 이를 이용한 광산배수 처리방법
KR100618305B1 (ko) 갱내 황산염 환원시설
James Gusek et al. The challenges of designing, permitting and building a 1,200 gpm passive bioreactor for metal mine drainage West Fork mine, Missouri
Klapper et al. Ways of controlling acid by ecotechnology
Simm et al. Biological treatment technologies
WO2019165567A1 (fr) Système et procédé de biorémédiation d'eaux contaminées
CN104961288B (zh) 生态氧化沟与生态箱湿地结合的污水处理系统
ES2363363B2 (es) Humedal artificial y uso del mismo para la fitopurificación de efluentes líquidos
Banjoko et al. Upgrading Wastewater Treatment Systems for Urban Water Reuse
KR100991694B1 (ko) 산성배수 처리에 사용되는 황산염 환원균의 활성화를 위한 기질물질 및 이를 이용한 산성배수 처리 장치
Saad et al. Management and treatment methods of acid mine drainage
US11104596B2 (en) Bioreactor, system, and method for reduction of sulfates from surface waters
du Preez Treatment of Acid Mine Drainage for Reuse in Irrigation
TW201016620A (en) Constructed wetland for treating saline wastewater
Vyawahre et al. Acid mine drainage: a case study of an Indian coal mine
Borkar et al. Tidal flow constructed wetland: An overview

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18907817

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18907817

Country of ref document: EP

Kind code of ref document: A1

122 Ep: pct application non-entry in european phase

Ref document number: 18907817

Country of ref document: EP

Kind code of ref document: A1