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WO2019018911A1 - Pellicule de fermeture étanche et procédé de pipetage faisant intervenir une pellicule de fermeture étanche - Google Patents

Pellicule de fermeture étanche et procédé de pipetage faisant intervenir une pellicule de fermeture étanche Download PDF

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Publication number
WO2019018911A1
WO2019018911A1 PCT/BR2018/050180 BR2018050180W WO2019018911A1 WO 2019018911 A1 WO2019018911 A1 WO 2019018911A1 BR 2018050180 W BR2018050180 W BR 2018050180W WO 2019018911 A1 WO2019018911 A1 WO 2019018911A1
Authority
WO
WIPO (PCT)
Prior art keywords
layer
sealing film
container
film
securing means
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/BR2018/050180
Other languages
English (en)
Portuguese (pt)
Inventor
Murilo CASTRO CERVATO
Felipe Camilo VAL
Gregorio Tadeu Fernando DASTOLI
Renato DE MELLO RUIZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein
Original Assignee
Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein filed Critical Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein
Publication of WO2019018911A1 publication Critical patent/WO2019018911A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials

Definitions

  • the present invention relates to a film provided with a plurality of layers, wherein one layer is configured to direct a pipetting process and another layer has the function of sealing, sealing and isolating a container provided with a plurality of wells when used in said pipetting process.
  • WO 02/081087 is initially outstanding.
  • This pro- provides a cover for PCR plates with a plurality of holes and a resilient layer cooperating with a rigid layer.
  • This cover includes a first layer being preferably a plastic film that is disposed on the surface of the plate to cover the holes, preventing the material therein from evaporating or contaminating, sealing said holes.
  • the attachment of this layer of plastic film can occur through adhesives, among other means.
  • the disadvantage of the proposal disclosed in this document is that the proposed cover is similar to a cover and does not have two independent layers, so that neither is mention of a second layer that can present graphic or visual indications about the location of the wells of the plate used.
  • US 2004/032093 proposes a method of producing an adhesive sealant as well as a adhesive sealant produced by said method.
  • the proposed sealant has a plurality of portions with metal blades and is provided with a surface having adhesive material in its entirety, so that it is integrally attached to the plate used in the laboratory procedure.
  • the film is integrally bonded to the plate in such a way that the film must be damaged or destroyed if it is desired to access the wells to continue the laboratory process.
  • a layer with graphic or visual cues is not provided which directs the technician to perform the procedure correctly.
  • US 2008/233015 describes a container sealer, made of elastomeric material and with layers of planar geometry and which are brought together to form said sealant. It is mentioned that a first layer is formed of materials of specific hardness wherein an optional second layer is formed of a material which provides support to the first layer and the attachment of the sealant to the plate is by heating said sealant at a temperature between 85 C and 1 Q 10 Q C in a heating cycle by a heating plate that presses the seal against the container.
  • the present invention proposes a sealing film for laboratories.
  • This film is basically composed of two independent layers, which help and guide the technician in the execution of laboratory processes, such as, by means of the visual and graphic indications of the exact location of the wells, as well as indicating if these have been previously used or not.
  • this film provides greater safety in laboratory environments, since the technician carrying out the process can precisely direct the instru- ments. for the well to be used, and that the possibility of using a well is wrongly reduced, since the well used will be marked.
  • a sealing film for laboratories provided with two independent layers, wherein a layer has indications as to the location of the wells, showing whether they have already been used or not and thus guaranteeing a method of pipetting safe and accurate.
  • the other layer has the function of ensuring a safe seal of the wells after the pipetting process.
  • a second object of the present invention is to provide a sealing film having visual and graphic indications on the positioning and use of wells to be used in a pipetting procedure.
  • a third object of the present invention is to provide a safe film that assists and directs the responsible technician during the pipetting (guided pipetting) procedure.
  • a fourth object of the present invention is to provide an effective and precise pipetting method which is carried out with the aid of visual and graphic indications and markings by means of the sealing film.
  • a fifth aim of the present invention is to provide a film which provides a safe seal, which prevents evaporation of products and promotes safety against contamination from well to well.
  • a sixth objective of the present invention is to provide a film having thermal, mechanical and optical properties that can be used in a variety of laboratory techniques such as conventional polymerase chain reaction (PCR), time PCR real, ELISA (Enzyme- Linked Immunosorbent Assay), cell culture, microarray, serology, agglutination, flow cytometry, MIC (Minimum Inhibitory Concentration), HPLC (High Performance Liquid Chromatography), mass spectrometry, or any multiwell plate having geometric compatibility.
  • PCR polymerase chain reaction
  • ELISA Enzyme- Linked Immunosorbent Assay
  • cell culture microarray, serology, agglutination, flow cytometry, MIC (Minimum Inhibitory Concentration), HPLC (High Performance Liquid Chromatography), mass spectrometry, or any multiwell plate having geometric compatibility.
  • sealing film and method of pipetting using a sealing film, wherein said sealing film comprises at least two layers, which are partially bonded together by a bonding portion configured to allow angular displacement independent of each of the layers, wherein a first layer is configured as a protection layer and a second layer is configured as a guide layer and further has a plurality of identification guides.
  • Figure 1 is a preferred representation of the sealant film proposed in the present invention, showing the second layer being removed as well as the identification guides disposed in this layer;
  • Figure 2 is a further representation of the sealant film proposed in the present invention, showing the second layer being removed and an alternative layout and indication of the identification guides;
  • Figure 3 is a representation of the securing means used in the sealing film proposed in the present invention
  • Figure 4 is a preferred representation of the sealing film proposed in the present invention, representing the removal of a protective membrane from the sealing film;
  • Figure 5 is a preferred representation of a step of the pipetting method proposed in the present invention using the sealing film, depicting its attachment in a container;
  • Figure 6 is a preferred representation of a step of the pipetting method proposed in the present invention using the sealing film, depicting a side view of the contact of a second layer with the container;
  • Figure 7 is a preferred representation of a step of the pipetting method proposed in the present invention using the sealing film, showing a front view of the contact of a second layer with the container;
  • Figure 8 is a preferred representation of a step of the pipetting method proposed in the present invention using the sealing film representing a perforation (pipetting) of a layer in the identification guides in a laboratory process preferably of pipetting;
  • Figure 9 is a preferred representation of a step of the pipetting method proposed in the present invention using the sealing film, representing a layer of the sealing film being lowered and covering the container used in the laboratory process.
  • the sealant film 1 is preferably used to seal and seal a container 2 provided with at least one well or a plurality of wells 3, as shown in Figures 1 and 2.
  • the sealing film 1 is composed of at least two layers 4, 5. More specifically, in order to compose the seal film 1, at least one first layer 4 and a second layer 5 are provided, but alternatively other additional intermediate layers may also be provided, as will be discussed below.
  • the first layer 4 is configured as a protective layer, so as to seal the container 2 and prevent its contents from being accessed by external agents, thus preventing it from being altered in any way , including by contamination, leakage, evaporation and addition of unwanted materials, for example.
  • the first layer 4 comprises at least one of a protective membrane 6 and a permanent layer 7.
  • the main layer 7 has the main function of closing, sealing and sealing the wells 3 of the container 2.
  • the protection membrane 6 is already intended to keep sterilized and clean a surface which will face towards the inside of the wells 3 of the container 2 a be sealed and / or protect a possible adhesive from the permanent layer 7 which will make the attachment to the container 2.
  • a lower surface of the permanent layer 7 will subsequently be in contact with the container 2 which it is desired to seal, such that the protective membrane 6 has as its main function to protect and isolate this surface, while maintaining -a free from external agents that may damage or infect it by contaminating contents inside the container 2.
  • the first layer 4 comprises an association of the protection membrane 6 and the permanent layer 7, this association being such that the protection membrane 6 is removable from the sealing film 1, as shown 3 and 4.
  • the first layer 4 is preferably comprised of a joint of the protection membrane 6 and the permanent layer 7, wherein the protection membrane 6 is associated with a lower surface of the permanent layer 7.
  • This association is preferably given by adhesive means such that said protection membrane 6 can be removed from the sealing film 1.
  • adhesive means represents only a preferred feature of the present invention.
  • the protection membrane 6 is preferably made of an opaque material, such as non-stick siliconized paper, but may also be made of elastomer or polyester and may alternatively be transparent.
  • the preparation of the permanent layer 7 occurs in a transparent material of high quality and supporting high temperatures, such as polyester-based elastomer or any other adherent material which avoids evaporation, spillage and contamination of the contents of the wells, alternatively it can be made of plastic, aluminum, latex and similar materials.
  • an alternative configuration includes the first layer 4 being composed of only one layer.
  • the first layer 4 is therefore formed by a single component, which may be made of opaque or transparent material.
  • the second layer 5 is configured as a guide layer.
  • the guide layer 5 (second layer 5) has a plurality of identification guides 8 on an upper surface. These identification guides 8 are configured as templates having cooperating shape with the shape of the wells 3 of the container 2 to be sealed.
  • the identification guides 8 have the same shape as the wells 3 of the container 2, so that said wells can be properly located through (through) the second of the layer 5 and thus a laboratory process used of the present invention can be performed correctly.
  • the identification guides 8 preferably comprise embossed indications of the shape and position of each well 3 of the container 2, also indicating whether this well has already been used or not. Such indication of the use of the wells 3 is easily observed by the user, since the already pipetted well 3 will present its respective identification guide 8 breached (perforated).
  • an identification guide 8 is provided for each well 3, the geometry and size of the identification guides 8 being sufficient to individually cover and cover an upper region of each well 3.
  • the arrangement and spacing of the identification guides 8 on the upper surface of the second layer 5 are in accordance with the type of container 2 to be sealed.
  • the arrangement and spacing of said guides 8 may vary according to the geometry and characteristics of the container 2, thus, the representation of figure 1 as a limitation of the configuration of the sealing film 1 and its guides 8 .
  • the identification tabs 8 are similarly disposed to a die on the upper surface of the second layer 5, in which there is an indication related to a line and a column referring to the exact location of each well 3 of the container 2.
  • line and column indications are referred to as symbols, as illustrated by the numerals "1 to 5" and letters "A” through “G” in Figures 1 and 2.
  • each identification guide 8 has a corresponding signaling.
  • These signals preferably comprise graphical indications (letters and numbers) relating to the exact location of the wells 8.
  • said signals comprise, for example, a first element and a second element, which can respectively be represented by a letter and a number.
  • a first element and a second element which can respectively be represented by a letter and a number.
  • one has the form: A1, A2, A3, B1, B2, B3, C1, C2, C3, etc. where the first elements "A”, “B” and “C” represent the line e the second elements "1", "2" and "3" represent the column.
  • the present invention allows such signs to be disposed as shown in Figure 1, ie, only at the edge of the second layer 5.
  • such identifications may be disposed on each of the guides 8, as depicted in Figure 2. Obviously , the combination of the two proposals is fully acceptable.
  • each signaling such as representation of Figures 1 and 2 should not be a limitation for the sealing film 1, such that other forms of signaling may be used, including alternatively, but not limited to, with high relief, with different colors, shapes, among others.
  • the second layer 5 is removable relative to the sealing film 1 which it composes. By removable the feature of the second layer 5 may be selectively disassociated from the sealing film 1. To this end, the second layer 5 can be detached from the sealing film 1 by a user after use.
  • the second layer 5 has a less resistant region to the user's action to remove it.
  • the second layer 5 has a region which has certain cuts, grooves or notches which facilitate its removal from the sealing film 1, according to Figures 1 and 2.
  • the second layer 5 is preferably made of a puncturable material.
  • a preferred embodiment uses the second layer 5 made, for example, in a thin layer of aluminum.
  • other adhesive or non-adhesive materials such as non-porous paper or any other materials which are easily pierceable may also be used.
  • an alternative configuration presents the second layer 5 made of perforable material, such as aluminum, only in said regions of the identification guides 8, such that the remaining of the second layer 5 not covered by these guides 8 is made of more resistant and not necessarily perforating materials such as rubber or plastic, for example.
  • the sealing film 1 may be provided with more than two layers, such that the number of layers may vary, provided there is at least one first layer 4 and a second layer 5 as described above.
  • the sealing film 1 includes a second protective membrane (not shown) which is coupled to an upper portion of the permanent layer 7 in order to protect a surface that will be facing the external environment. From this this first layer 4 is provided with two protective membranes.
  • the sealant film 1 is composed of four components, a first layer 4 being composed of two protection membranes 6, a permanent layer 7 and a second layer 5.
  • a connecting portion 9 As disclosed in Figures 1 and 2.
  • This main portion 9 has the main function of partially joining the first layer 4 and the second layer 5 in such a way that they can move independently, i.e., the first layer 4 can be moved relative to the second layer 5, and vice versa.
  • the sealing film 1 proposed in the present invention further comprises fastening means 10 disposed preferably on at least one end of the sealing film 1.
  • Such securing means 10 comprises side flaps configured to provide an association of the sealing film 1 to the container 2 to be sealed.
  • the securing means 10 is preferably arranged in at least one end of the sealing film 1.
  • the sealing film 1 has two securing means 10, each being disposed in a peripheral portion of said sealing film 1.
  • the arrangement of the securing means 10 occurs in such a way that two opposing side portions of the seal film 1 are provided with a securing means 10 each.
  • the securing means 10 are adhesive means and each has at least one securing strip 11, as shown in Figure 3.
  • securing means 10 may be provided in any side portions, other than those mentioned above. Further, the securing means 10 may be arranged in a same side portion, however, segmented in more than one part.
  • securing means 10 as adhesive elements represents only a preferred aspect of the invention.
  • other media could be used, such as specific fasteners that would be decoupled after use.
  • the use of the film 1 occurs in a method of pipetting performed with the aid of a container 2, which container is to be closed, sealed and sealed.
  • the pipetting method in the container 2 utilizes the sealant film 1 having the features described above, wherein said film 1 comprises at least two layers 4, 5 partially joined to one another by a portion of and further comprising securing means 10 disposed on at least one of its ends, configured to provide the association of the seal film 1 to the container 2 to be sealed.
  • the pipetting method proposed in the present invention preferably comprises the steps described below:
  • a first step comprises securing the sealing film 1 to a portion of the container 2, as shown in Figure 5.
  • this step comprises attaching the sealing film 1 to the container 2 to be sealed. This association takes place through the securing means 10 preferably arranged in at least one of the ends of the sealing film 1, which are preferably glued to the container 2.
  • the securing means has at least one securing strip 11, which must be removed prior to the association of the securing means 10 with the container 2.
  • a second step comprises holding the second layer 5 in contact with the container 2, as shown in Figures 6 and 7. To this end, the second layer 5 must be moved (lowered and / or glued) until it touches the container 2.
  • a third step comprises raising the first layer 4, allowing the visualization of a plurality of identification guides 8 arranged in the second layer 5, as shown in Figures 6 and 7.
  • this step consists in maintaining the second layer (raised) the first layer 4 so as to visualize the plurality of identification guides 8.
  • the guides 8 comprise signs associated with each of them, as shown in figures 1 and 2.
  • a fourth step comprises (pipetting) the second layer 5 in the identification guides 8, in a laboratory process preferably of pipetting. This step is shown in figure 8.
  • An optional fifth step comprises detaching the second layer 5 of the attachment portion 9, removing said second layer 5 from the sealing film 1, as shown in Figures 1 and 2.
  • a final step comprises lowering the first layer 4 by covering a portion of the container 2.
  • the container 2 may also be covered in its entirety, if necessary.
  • this step may be preceded by a step which comprises removing the securing means 10 and the membrane 6 which protects the permanent layer 7, as shown in Figure 4.
  • the present invention describes a sealing film 1 which comprises two independent and detachable layers, layers that aid and direct the technician in carrying out laboratory processes, such as pipetting, in addition to protecting against mechanical actions, to prevent leaks and / or contaminations from well to well, to withstand high temperatures and to possess optical properties in order to be used in the reactions of the most different laboratory areas.
  • the use of the identification guides 8 and their respective signaling guarantees that each pipetting will be carried out in the correct well, while also ensuring that the technician can easily visualize each well that has already been used. In this way, greater safety and speed in the pipetting process are obtained from the teachings of the present invention.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention concerne une pellicule de fermeture étanche (1) et un procédé de pipetage faisant intervenir une pellicule de fermeture étanche (1), ladite pellicule de fermeture étanche (1) comprenant au moins deux couches (4, 5) partiellement jointes l'une à l'autre par une partie de jonction (9) conçue pour permettre un déplacement angulaire indépendant de chacune des couches (4, 5), une première couche (4) étant conçue comme une couche de protection et une seconde couche (5) étant conçue comme une couche de guidage et possédant en outre une pluralité de guides d'identification (8).
PCT/BR2018/050180 2017-07-26 2018-06-01 Pellicule de fermeture étanche et procédé de pipetage faisant intervenir une pellicule de fermeture étanche Ceased WO2019018911A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BR102017015998-1A BR102017015998A2 (pt) 2017-07-26 2017-07-26 Película seladora e método de pipetagem utilizando uma película seladora
BR102017015998-1 2017-07-26

Publications (1)

Publication Number Publication Date
WO2019018911A1 true WO2019018911A1 (fr) 2019-01-31

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PCT/BR2018/050180 Ceased WO2019018911A1 (fr) 2017-07-26 2018-06-01 Pellicule de fermeture étanche et procédé de pipetage faisant intervenir une pellicule de fermeture étanche

Country Status (2)

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BR (1) BR102017015998A2 (fr)
WO (1) WO2019018911A1 (fr)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0282840A2 (fr) * 1987-03-17 1988-09-21 Becton, Dickinson and Company Dispositif à usage unique à utiliser pour des analyses chimiques, immunochimiques et microbiologiques
JPH0445856A (ja) * 1990-06-14 1992-02-14 Toshimasa Akiyama ピペットチップに着ける薄膜構造
CA2130129A1 (fr) * 1993-09-10 1995-03-11 Martin Walter Ellenberger Fermeture a zone de perforation
DE19618773C1 (de) * 1996-05-10 1997-11-06 Sbf Wasser Und Umwelt Zweignie Verfahren und Vorrichtung zur Probenvorbereitung und Messung des Elementgehaltes in eindampfbaren Flüssigkeiten
CN102153926A (zh) * 2011-02-15 2011-08-17 山西省交通科学研究院 一种沥青路面渗透再生涂料及其制备方法
KR20130074967A (ko) * 2011-12-27 2013-07-05 (주)엘지하우시스 다층 구조의 전자종이용 실링필름 및 이를 이용한 전자종이 장치의 제조방법
CN203546998U (zh) * 2013-11-08 2014-04-16 浙江嘉宜实业有限公司 一种顶棚转动锁紧机构
US20150355149A1 (en) * 2012-12-20 2015-12-10 Merck Patent Gmbh Method and device for carrying out thin-layer chromatography
WO2016130553A1 (fr) * 2015-02-13 2016-08-18 Siemens Healthcare Diagnostics Inc. Procédés et appareil permettant de réduire les volumes rapportés durant les opérations de pipetage

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0282840A2 (fr) * 1987-03-17 1988-09-21 Becton, Dickinson and Company Dispositif à usage unique à utiliser pour des analyses chimiques, immunochimiques et microbiologiques
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