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WO2019017691A3 - Procédé de différenciation de cellule souche pluripotente induite humaine en cellule précurseur de papille dermique et son utilisation - Google Patents

Procédé de différenciation de cellule souche pluripotente induite humaine en cellule précurseur de papille dermique et son utilisation Download PDF

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Publication number
WO2019017691A3
WO2019017691A3 PCT/KR2018/008123 KR2018008123W WO2019017691A3 WO 2019017691 A3 WO2019017691 A3 WO 2019017691A3 KR 2018008123 W KR2018008123 W KR 2018008123W WO 2019017691 A3 WO2019017691 A3 WO 2019017691A3
Authority
WO
WIPO (PCT)
Prior art keywords
dermal papilla
precursor cell
differentiation
human
papilla precursor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/008123
Other languages
English (en)
Korean (ko)
Other versions
WO2019017691A2 (fr
WO2019017691A9 (fr
Inventor
권오상
강보미
김진용
권유욱
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SNU R&DB Foundation
Original Assignee
Seoul National University R&DB Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seoul National University R&DB Foundation filed Critical Seoul National University R&DB Foundation
Priority to US16/632,472 priority Critical patent/US11629331B2/en
Priority claimed from KR1020180083386A external-priority patent/KR102122039B1/ko
Publication of WO2019017691A2 publication Critical patent/WO2019017691A2/fr
Publication of WO2019017691A3 publication Critical patent/WO2019017691A3/fr
Publication of WO2019017691A9 publication Critical patent/WO2019017691A9/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une composition de type milieu pour la différenciation d'une cellule souche pluripotente induite humaine en une cellule précurseur de papille dermique, un procédé de différenciation et une utilisation pour induire la néogenèse de follicules pileux à l'aide de ladite cellule précurseur de papille dermique différenciée. Les présents inventeurs ont mis au point un procédé de différenciation d'une cellule souche pluripotente induite humaine en une cellule précurseur de papille dermique ayant une capacité de formation de follicules pileux et une composition de type milieu de différenciation spécifique de cellule précurseur de papille dermique pour la différenciation ci-dessus, et ont induit efficacement la néogenèse de follicules pileux constitués uniquement de cellules humaines, sans follicules pileux hybrides murins-humains classiques, à l'aide de ladite cellule précurseur de papille dermique induite humaine et d'une cellule précurseur d'épiderme induite humaine obtenue par l'intermédiaire dudit procédé de différenciation. Le tissu folliculaire pileux humain obtenu par application de la technique de différenciation de la cellule précurseur de papille dermique selon la présente invention devrait être utile à titre de procédé thérapeutique pour les patients souffrant de perte de cheveux en surmontant les limites des traitements contre la perte des cheveux.
PCT/KR2018/008123 2017-07-19 2018-07-18 Procédé de différenciation de cellule souche pluripotente induite humaine en cellule précurseur de papille dermique et son utilisation Ceased WO2019017691A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/632,472 US11629331B2 (en) 2017-07-19 2018-07-18 Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2017-0091727 2017-07-19
KR20170091727 2017-07-19
KR10-2018-0083386 2018-07-18
KR1020180083386A KR102122039B1 (ko) 2017-07-19 2018-07-18 인간 유래 만능줄기세포에서 모유두전구세포로의 분화방법 및 이의 용도

Publications (3)

Publication Number Publication Date
WO2019017691A2 WO2019017691A2 (fr) 2019-01-24
WO2019017691A3 true WO2019017691A3 (fr) 2019-03-28
WO2019017691A9 WO2019017691A9 (fr) 2019-05-09

Family

ID=65015799

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/008123 Ceased WO2019017691A2 (fr) 2017-07-19 2018-07-18 Procédé de différenciation de cellule souche pluripotente induite humaine en cellule précurseur de papille dermique et son utilisation

Country Status (1)

Country Link
WO (1) WO2019017691A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4022037A1 (fr) * 2019-07-10 2022-07-06 Helmuth Heinrich Kunz Procédés de dérivation in vitro de feuilles contenant des follicules pileux autologues et hypo-immunogènes
CN115418341B (zh) * 2022-09-22 2024-03-15 北京雍禾医疗投资管理有限公司 成纤维细胞向毛乳头细胞转分化的方法及其应用
KR102543431B1 (ko) * 2022-10-05 2023-06-15 주식회사 프롬바이오 지방 유래 줄기세포의 모유두세포로의 분화 유도용 조성물 및 그 조성물을 이용한 분화 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016114723A1 (fr) * 2015-01-16 2016-07-21 Agency For Science, Technology And Research Différenciation de macrophages à partir de cellules souches pluripotentes
KR20160145170A (ko) * 2014-04-21 2016-12-19 카오카부시키가이샤 피부 유래 전구 세포의 제작 방법
WO2017070506A1 (fr) * 2015-10-21 2017-04-27 Indiana University Research And Technology Corporation Dérivation d'organoïdes cutanés humains à partir de cellules souches pluripotentes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160145170A (ko) * 2014-04-21 2016-12-19 카오카부시키가이샤 피부 유래 전구 세포의 제작 방법
WO2016114723A1 (fr) * 2015-01-16 2016-07-21 Agency For Science, Technology And Research Différenciation de macrophages à partir de cellules souches pluripotentes
WO2017070506A1 (fr) * 2015-10-21 2017-04-27 Indiana University Research And Technology Corporation Dérivation d'organoïdes cutanés humains à partir de cellules souches pluripotentes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GNEDEVA, KSENIA ET AL.: "Derivation of Hair-inducing Cell from Human Pluripotent Stem Cells", PLOS ONE, vol. 10, no. 1, 2015, pages 1 - 14, XP055584890 *
IN AMATSU, MUTSUMI ET AL.: "Embryonic Dermal Condensation and Adult Dermal Papilla Induce Hair Follicles in Adult Glabrous Epidermis through Different Mechanisms", DEVELOPMENT, GROWTH & DIFFERENTIATION, vol. 48, 2006, pages 73 - 86, XP055584889 *
KIM, J. ET AL.: "Human Hair Follicle Regeneration with Trichogenic Human Dermal Papilla Precursor Cells Derived from Induced Pluripotent Stem Cells", 76TH ANNUAL MEETING PORTLAND OREGON OF SOCIETY FOR INVESTIGATIVE DERMATOLOGY, 27 April 2017 (2017-04-27) *
VERAITCH, OPHELIA ET AL.: "Induction of Hair Follicle Dermal Papilla Cell Properties in Human Induced Pluripotent Stem Cell -derived Multipotent LNGFR(+)THY-1(+) Mesenchymal Cells", SCIENTIFIC REPORTS, vol. 7, no. 42777, 21 February 2017 (2017-02-21), pages 1 - 13, XP055446550 *

Also Published As

Publication number Publication date
WO2019017691A2 (fr) 2019-01-24
WO2019017691A9 (fr) 2019-05-09

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