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WO2019008679A1 - Échantillon biologique simulé - Google Patents

Échantillon biologique simulé Download PDF

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Publication number
WO2019008679A1
WO2019008679A1 PCT/JP2017/024533 JP2017024533W WO2019008679A1 WO 2019008679 A1 WO2019008679 A1 WO 2019008679A1 JP 2017024533 W JP2017024533 W JP 2017024533W WO 2019008679 A1 WO2019008679 A1 WO 2019008679A1
Authority
WO
WIPO (PCT)
Prior art keywords
shape
simulation sample
tissue
biological simulation
biological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2017/024533
Other languages
English (en)
Japanese (ja)
Inventor
裕基 庄野
成田　利治
明日香 向井
遼佑 伊藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Priority to PCT/JP2017/024533 priority Critical patent/WO2019008679A1/fr
Priority to PCT/JP2018/025179 priority patent/WO2019009279A1/fr
Publication of WO2019008679A1 publication Critical patent/WO2019008679A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine

Definitions

  • the present invention relates to a biological simulation sample, and more particularly to a biological simulation sample for fluorescence observation.
  • a method of injecting a fluorescent dye such as indocyanine green (ICG) into a patient and measuring the fluorescence intensity from the fluorescent dye in blood is known as a means for determining the presence or absence of blood flow in living tissue.
  • a standard sample that emits fluorescence of known intensity is used to standardize the measured intensity of fluorescence (see, for example, Patent Documents 1 and 2).
  • Patent Documents 1 and 2 are not suitable for use as simulated samples for performance evaluation and demonstration of endoscopes. That is, in the performance evaluation and demonstration of the endoscope, while using a simulated sample instead of the living tissue in the living body, the appearance of the fluorescence from the living tissue in the image acquired by the endoscope in the clinical scene is as real as possible It is required to reproduce in However, since the standard samples of Patent Documents 1 and 2 do not simulate living tissue, they can not achieve a near-visible appearance of fluorescence from living tissue in a clinical setting.
  • the present invention has been made in view of the above-described circumstances, and it is an object of the present invention to provide a biological simulation sample capable of realizing the appearance close to the appearance of fluorescence from biological tissue in a clinical situation.
  • One aspect of the present invention is a first member containing a fluorescent material and simulating the shape of a living tissue to be observed, and a second member covering the first member and simulating the optical characteristics of the surrounding tissue of the living tissue to be observed And a biological simulation sample comprising
  • the shape of the living tissue to be observed is simulated by the first member, and the optical property of the peripheral tissue to be observed is simulated by the second member around the first member. Therefore, by irradiating the biological simulation sample with excitation light to cause the fluorescent material contained in the first member to emit light, it is possible to realize a near-visible appearance of fluorescence from biological tissue in a clinical situation.
  • the second member has a layer structure in which two or more layers are stacked, and the two or more layers are different from each other in at least one of the light scattering property and the light absorption property. Good.
  • Such a second member more realistically simulates the optical characteristics of the surrounding tissue having a layered structure. This makes it possible to achieve a view closer to the view of the living tissue to be observed in the surrounding tissue having a layered structure.
  • the first member may simulate the shape of a vessel, for example, the running shape of a blood vessel or the shape of a lymphatic vessel. In this way, a closer view of fluorescence from vessels such as blood vessels or lymph vessels in a clinical setting can be realized by the first member simulating the shape of the vessel.
  • the fluorescent material may be indocyanine green (ICG).
  • ICG indocyanine green
  • ICG is often used for fluorescence observation of vessels in the clinic. Therefore, a closer view of the fluorescence from vessels in a clinical setting can be achieved by the first member including the ICG.
  • the first member is made of a gel material containing a fluorescent material
  • the second member is made of a cured resin material
  • the first member is embedded in the second member. It is also good.
  • a gel material that can be shaped into any shape, various biological tissue shapes can be simulated by the first member.
  • the gel material can be prevented from drying and the biological simulation sample can be stored for a long time.
  • ADVANTAGE OF THE INVENTION According to this invention, it is effective in the ability to implement
  • FIG. 1A It is a perspective view of a living body simulation sample concerning one embodiment of the present invention. It is a side view of the biological simulation sample of FIG. 1A. It is a top view of the living body simulation sample which shows the modification of the shape of the 1st member of Drawing 1A. It is a top view of the living body simulation sample which shows the other modification of the shape of the 1st member of Drawing 1A. It is a perspective view of the modification of a living body simulation sample of Drawing 1A. It is a side view of a living body simulation sample of Drawing 4A. It is a perspective view of the other modification of the biological simulation sample of FIG. 1A.
  • the living body simulation sample 1 is used as a subject instead of a living tissue in a living body in the evaluation and demonstration of the image performance of an endoscope.
  • the biological simulation sample 1 includes the first member 2 simulating the shape of the biological tissue to be observed, and the optical characteristics of the surrounding tissue of the biological tissue to be observed covering the first member 2 And a second member 3 that simulates.
  • the first member 2 is made of a gel material containing indocyanine green (ICG).
  • ICG indocyanine green
  • a gel material for example, an agar gel, a gelatin gel, a two-component mixed gel, or the like is used.
  • the first member 2 has a shape that simulates the traveling shape of a blood vessel to be observed.
  • the first member 2 has a branch structure in which branching from one linear structure to a plurality of linear structures is repeated. In FIG. 1A, the shape of the first member 2 is simplified.
  • the second member 3 is made of a block-like or sheet-like cured resin material, and the first member 2 is embedded in the second member 3.
  • resin material silicone resin, polyurethane resin, epoxy resin or the like is used.
  • the second member 3 is the same as or similar to the surrounding tissue to be observed in at least one of the light absorption property and the light scattering property.
  • Such a biological simulation sample 1 is manufactured, for example, by the following method.
  • the gel material is uniformly mixed with ICG, the gel material is filled in a syringe, and the gel material is discharged in a thread form from the syringe, thereby forming the gel material into a shape that simulates the traveling shape of a blood vessel.
  • a liquid resin material is poured into a mold, the entire molded gel material is placed inside the resin material, and the resin material is cured. Thereby, the biological simulation sample 1 shown in FIG. 1A is manufactured.
  • the biological simulation sample 1 is disposed to face the tip of the endoscope, the excitation light (wavelength 760 nm) is irradiated to the biological simulation sample 1 from the endoscope, and the fluorescence (wavelength) emitted from the ICG contained in the first member 2 830 nm) is observed by an endoscope.
  • an image (simulated image) simulating an image obtained by imaging fluorescence from a blood vessel in a living body to which ICG has been administered in a clinical scene is acquired by the endoscope.
  • the acquired simulated image of the biological simulation sample 1 is displayed on the display.
  • the user evaluates the imaging performance of the endoscope during clinical use based on the simulated image displayed on the display.
  • the first member 2 simulates the traveling shape of the blood vessel in the living body
  • fluorescence is emitted in the same shape as the traveling shape of the blood vessel.
  • the second member 3 around the first member 2 simulates the optical properties of the surrounding tissue of the blood vessel
  • the fluorescence emitted from the first member 2 is the same as in living tissue in the second member 3 Spread out.
  • the appearance close to the appearance of the fluorescence observed by the endoscope in the clinical scene is realized.
  • the user can accurately evaluate the appearance of fluorescence in the image when the endoscope is used clinically based on the simulated image on the display.
  • the first member 2 is made of a gel material that can be formed into an arbitrary shape, the shape of any living tissue including a living tissue having a complicated shape such as a traveling shape of a blood vessel Has the advantage of being able to simulate
  • the gel material constituting the first member 2 is not suitable for long-term storage since the characteristics change due to drying etc., the cured resin material constituting the second member 3 is stable against air and moisture. Material and suitable for long-term storage. Therefore, covering the entire first member 2 with the second member 3 has an advantage of being able to provide the biological simulation sample 1 that can be stored for a long time while maintaining the quality of the fluorescent material.
  • the first member 2 simulates the traveling shape of the blood vessel.
  • the shape of another living tissue may be simulated.
  • the first member 2 may simulate the shape of a lymphatic vessel and may simulate the shape of another vessel such as a bile duct.
  • the first member 2 may simulate the shape of a tumor.
  • ICG is used as the fluorescent material, but the type of fluorescent material is not limited to this, and other types of fluorescent materials may be used.
  • fluorescent material quantum dots or protoporphyrin IX (ppIX) may be used as the fluorescent material.
  • ppIX protoporphyrin IX
  • ppIX protoporphyrin IX
  • the single first member 2 is disposed in the second member 3 having a single-layer structure
  • the number of first members 2 and the number of layers constituting the second member 3 can be changed as appropriate.
  • the plurality of first members 21 and 22 may be disposed at mutually different depths in the second member 3 having a single-layer structure.
  • the shapes of the plurality of first members 21 and 22 may be identical to each other, or may be different from each other as shown in FIG. 4A.
  • the second member 3 may have a layered structure in which two or more layers 3 a and 3 b are stacked.
  • the 2nd member 3 of 2 layer structure is shown by FIG. 5 as an example.
  • Surrounding tissues such as blood vessels, lymph vessels, and tumors have a layered structure composed of multiple layers having different light scattering properties and light absorption properties.
  • the layers 3a and 3b of the second member 3 are different from each other in at least one of the light scattering property and the light absorption property so as to simulate the optical property of each layer (for example, the mucous layer and muscle layer) of the surrounding tissue. . This makes it possible to achieve an appearance closer to the appearance of fluorescence from the observation target in a clinical setting.
  • the layer 3a, 3b have respectively about 0.1 mm -1 or more 5 mm -1 or less of the light scattering coefficient in the wavelength range of visible light.
  • the layers 3a and 3b have light absorption characteristics that simulate the absorption spectrum of blood, and each have a light absorption coefficient of more than about 0 mm -1 and 20 mm -1 or less in the visible light wavelength range.
  • the light scattering coefficient and the light absorption coefficient of the layer 3b simulating the layer on the deep side are preferably larger than the light scattering coefficient and the light absorption coefficient of the layer 3a simulating the layer on the front surface, respectively.
  • the first member 2 may be disposed only in one layer, and is disposed between two adjacent layers. It may be provided, or may be disposed across multiple layers. Alternatively, the first member 2 may be disposed in each of the layers 3a and 3b.

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Algebra (AREA)
  • Pure & Applied Mathematics (AREA)
  • Business, Economics & Management (AREA)
  • Educational Administration (AREA)
  • Educational Technology (AREA)
  • Theoretical Computer Science (AREA)
  • Mathematical Optimization (AREA)
  • Medicinal Chemistry (AREA)
  • Mathematical Analysis (AREA)
  • Optics & Photonics (AREA)
  • Computational Mathematics (AREA)
  • Radiology & Medical Imaging (AREA)
  • Mathematical Physics (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Molecular Biology (AREA)
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  • Public Health (AREA)
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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Endoscopes (AREA)

Abstract

L'invention concerne un échantillon biologique simulé (1) comprenant : un premier élément (2) qui comprend un matériau fluorescent et simule la forme d'un tissu biologique devant être observé ; et un second élément (3) qui recouvre le premier élément (2) et simule les propriétés optiques du tissu entourant le tissu biologique devant être observé.
PCT/JP2017/024533 2017-07-04 2017-07-04 Échantillon biologique simulé Ceased WO2019008679A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/JP2017/024533 WO2019008679A1 (fr) 2017-07-04 2017-07-04 Échantillon biologique simulé
PCT/JP2018/025179 WO2019009279A1 (fr) 2017-07-04 2018-07-03 Échantillon biologique simulé, système d'évaluation d'endoscope et procédé d'évaluation d'endoscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2017/024533 WO2019008679A1 (fr) 2017-07-04 2017-07-04 Échantillon biologique simulé

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WO2019008679A1 true WO2019008679A1 (fr) 2019-01-10

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PCT/JP2017/024533 Ceased WO2019008679A1 (fr) 2017-07-04 2017-07-04 Échantillon biologique simulé
PCT/JP2018/025179 Ceased WO2019009279A1 (fr) 2017-07-04 2018-07-03 Échantillon biologique simulé, système d'évaluation d'endoscope et procédé d'évaluation d'endoscope

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020148533A (ja) * 2019-03-12 2020-09-17 太平洋セメント株式会社 石炭灰中の未燃炭素の評価方法
WO2025186219A1 (fr) 2024-03-05 2025-09-12 Compagnie Generale Des Etablissements Michelin Composition de caoutchouc comprenant un elastomere dienique fortement sature

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013096920A (ja) * 2011-11-02 2013-05-20 Hamamatsu Photonics Kk 蛍光ファントム装置および蛍光イメージング方法
WO2015037055A1 (fr) * 2013-09-10 2015-03-19 株式会社島津製作所 Dispositif d'acquisition d'image fluorescente
US20160310010A1 (en) * 2013-12-06 2016-10-27 Agency For Science, Technology And Research Method of imaging living tissue

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JP5539841B2 (ja) * 2010-10-26 2014-07-02 富士フイルム株式会社 電子内視鏡システム、電子内視鏡システムのプロセッサ装置、及び電子内視鏡システムの作動方法
US10024785B2 (en) * 2015-06-19 2018-07-17 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Solid hemoglobin-polymer biophotonic phantoms and their use

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2013096920A (ja) * 2011-11-02 2013-05-20 Hamamatsu Photonics Kk 蛍光ファントム装置および蛍光イメージング方法
WO2015037055A1 (fr) * 2013-09-10 2015-03-19 株式会社島津製作所 Dispositif d'acquisition d'image fluorescente
US20160310010A1 (en) * 2013-12-06 2016-10-27 Agency For Science, Technology And Research Method of imaging living tissue

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AKIYAMA, KOJI ET AL.: "Estimation of depth distribution of absorption by time-resolved measurement of backscattered light", IEICE TECHNICAL REPORT, vol. 102, no. 727, 11 March 2003 (2003-03-11), pages 1 - 4 *
ENOMOTO, TAKAHIRO ET AL.: "Fundamental study to realize optical imaging of arteriovenous fistula with PSF", IEICE TECHNICAL REPORT, vol. 114, no. 408, 15 January 2015 (2015-01-15), pages 105 - 109 *
HORINAKA, HIROMICHI ET AL.: "Detection of the target material in biological tissue by optically assisted ultrasonic velocity-change imaging method", JOURNAL OF MEDICAL ULTRASONICS, THE JAPAN SOCIETY OF ULTRASONICS IN MEDICINE, vol. 36, 15 April 2009 (2009-04-15), pages S292 *
SASAKI, HIROTAKA ET AL.: "Fundamental Study for reconstruction of in vivo fluorescent tomographic image using orthogonal function system", IEICE TECHNICAL REPORT, vol. 114, no. 515, 9 March 2015 (2015-03-09), pages 41 - 46 *
TANIGAWA, SHOHEI ET AL.: "Diagnosis device of vessel plaque using optically assisted ultrasonic velocity-change", EXTENDED ABSTRACTS OF THE 61ST JSAP SPRING MEETING, 2014, THE JAPAN SOCIETY OF APPLIED PHYSICS, 3 March 2014 (2014-03-03), pages 3 - 135 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020148533A (ja) * 2019-03-12 2020-09-17 太平洋セメント株式会社 石炭灰中の未燃炭素の評価方法
JP7197408B2 (ja) 2019-03-12 2022-12-27 太平洋セメント株式会社 石炭灰中の未燃炭素の評価方法
WO2025186219A1 (fr) 2024-03-05 2025-09-12 Compagnie Generale Des Etablissements Michelin Composition de caoutchouc comprenant un elastomere dienique fortement sature

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