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WO2019004780A3 - Novel o-succinyl homoserine transferase mutant and method for producing o-succinyl homoserine using same - Google Patents

Novel o-succinyl homoserine transferase mutant and method for producing o-succinyl homoserine using same Download PDF

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Publication number
WO2019004780A3
WO2019004780A3 PCT/KR2018/007409 KR2018007409W WO2019004780A3 WO 2019004780 A3 WO2019004780 A3 WO 2019004780A3 KR 2018007409 W KR2018007409 W KR 2018007409W WO 2019004780 A3 WO2019004780 A3 WO 2019004780A3
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WIPO (PCT)
Prior art keywords
succinyl homoserine
novel
producing
same
transferase mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/007409
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French (fr)
Korean (ko)
Other versions
WO2019004780A2 (en
Inventor
김경림
심지현
김현아
신용욱
이베드로
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
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CJ CheilJedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp
Priority to JP2019571997A priority Critical patent/JP6946479B2/en
Priority to RU2020100480A priority patent/RU2747494C1/en
Priority to US16/627,572 priority patent/US10982245B2/en
Priority to BR112019028095-2A priority patent/BR112019028095B1/en
Priority to CN201880044236.1A priority patent/CN111032862B/en
Priority to SG11201913899UA priority patent/SG11201913899UA/en
Priority to EP18822856.3A priority patent/EP3647415B1/en
Publication of WO2019004780A2 publication Critical patent/WO2019004780A2/en
Publication of WO2019004780A3 publication Critical patent/WO2019004780A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C319/00—Preparation of thiols, sulfides, hydropolysulfides or polysulfides
    • C07C319/02—Preparation of thiols, sulfides, hydropolysulfides or polysulfides of thiols
    • C07C319/08—Preparation of thiols, sulfides, hydropolysulfides or polysulfides of thiols by replacement of hydroxy groups or etherified or esterified hydroxy groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C319/00—Preparation of thiols, sulfides, hydropolysulfides or polysulfides
    • C07C319/14—Preparation of thiols, sulfides, hydropolysulfides or polysulfides of sulfides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01031—Homoserine O-acetyltransferase (2.3.1.31)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02004—Aspartate kinase (2.7.2.4)
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    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne un nouveau mutant d'O-succinyl homosérine transférase , un polynucléotide codant pour celui-ci, un microorganisme comprenant ce mutant et un procédé de production d'O-succinyl homosérine à l'aide du microorganisme.The present invention relates to a novel O-succinyl homoserine transferase mutant, a polynucleotide encoding it, a microorganism comprising this mutant and a method for producing O-succinyl homoserine using the microorganism.

PCT/KR2018/007409 2017-06-30 2018-06-29 Novel o-succinyl homoserine transferase mutant and method for producing o-succinyl homoserine using same Ceased WO2019004780A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2019571997A JP6946479B2 (en) 2017-06-30 2018-06-29 A novel O-succinyl homoserine transferase mutant and a method for producing O-succinyl homoserine using the same.
RU2020100480A RU2747494C1 (en) 2017-06-30 2018-06-29 New o-succinyl homoserine transferase mutant and method for synthesis of o-succinyl homoserine using said mutant
US16/627,572 US10982245B2 (en) 2017-06-30 2018-06-29 O-succinyl homoserine transferase mutant and method for producing O-succinyl homoserine using same
BR112019028095-2A BR112019028095B1 (en) 2017-06-30 2018-06-29 INNOVATIVE O-SUCCINYL HOMOSERIN TRANSFERASE MUTANT AND METHOD FOR THE PRODUCTION OF O-SUCCINYL HOMOSERINE USING THE SAME
CN201880044236.1A CN111032862B (en) 2017-06-30 2018-06-29 Novel O-succinylhomoserine transferase mutant and method for producing O-succinylhomoserine using the same
SG11201913899UA SG11201913899UA (en) 2017-06-30 2018-06-29 Novel o-succinyl homoserine transferase mutant and method for producing o-succinyl homoserine using same
EP18822856.3A EP3647415B1 (en) 2017-06-30 2018-06-29 Novel o-succinyl homoserine transferase mutant and method for producing o-succinyl homoserine using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0083439 2017-06-30
KR20170083439 2017-06-30

Publications (2)

Publication Number Publication Date
WO2019004780A2 WO2019004780A2 (en) 2019-01-03
WO2019004780A3 true WO2019004780A3 (en) 2019-03-28

Family

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PCT/KR2018/007409 Ceased WO2019004780A2 (en) 2017-06-30 2018-06-29 Novel o-succinyl homoserine transferase mutant and method for producing o-succinyl homoserine using same

Country Status (9)

Country Link
US (1) US10982245B2 (en)
EP (1) EP3647415B1 (en)
JP (1) JP6946479B2 (en)
KR (1) KR102112208B1 (en)
CN (1) CN111032862B (en)
BR (1) BR112019028095B1 (en)
RU (1) RU2747494C1 (en)
SG (1) SG11201913899UA (en)
WO (1) WO2019004780A2 (en)

Families Citing this family (3)

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Publication number Priority date Publication date Assignee Title
EP3991983B1 (en) 2019-06-28 2025-04-09 FUJIFILM Corporation On-press development type lithographic printing plate precursor, method for manufacturing a lithographic printing plate, and lithographic printing method
KR102277404B1 (en) * 2021-01-27 2021-07-14 씨제이제일제당 주식회사 Novel galactoside O-acetyltransferase variant and a method for producing L-glutamic acid using the same
CN116656634A (en) * 2023-06-02 2023-08-29 江南大学 Dioxygenase mutant and application thereof in synthesis of (2S, 3R) -3-hydroxy piperidine acid

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2015060649A1 (en) * 2013-10-23 2015-04-30 씨제이제일제당 (주) Microorganism producing o-succinyl homoserine and method for producing o-succinyl homoserine by using same
US20150211034A1 (en) * 2006-07-28 2015-07-30 Cj Cheiljedang Corporation Microorganism Producing L-Methionine Precursor and Method of Producing L-Methionine and Organic Acid from the L-Methionine Precursor
WO2016179545A1 (en) * 2015-05-06 2016-11-10 Trelys, Inc. Compositions and methods for biological production of methionine

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JP4623825B2 (en) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 Novel polynucleotide
KR100620092B1 (en) 2004-12-16 2006-09-08 씨제이 주식회사 Novel promoter sequences derived from Corynebacterium spp., Expression cassettes and vectors comprising the same, host cells comprising the vector and methods of expressing genes using the same
EP1907559A1 (en) * 2005-07-18 2008-04-09 Basf Se Methionine producing recombinant microorganisms
US9109242B2 (en) 2006-09-15 2015-08-18 Cj Cheiljedang Corporation Corynebacteria having enhanced L-lysine productivity and a method of producing L-lysine using the same
KR100930203B1 (en) 2008-01-28 2009-12-07 씨제이제일제당 (주) Improved promoter and method for producing L-lysine using the same
KR101048593B1 (en) 2009-02-27 2011-07-12 씨제이제일제당 (주) How to increase methionine production using a mixture of methylmercaptan and dimethylsulfide
WO2012087039A2 (en) * 2010-12-21 2012-06-28 씨제이제일제당 (주) Variant polypeptide having homoserine acetyltransferase activity and microorganism expressing same

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US20150211034A1 (en) * 2006-07-28 2015-07-30 Cj Cheiljedang Corporation Microorganism Producing L-Methionine Precursor and Method of Producing L-Methionine and Organic Acid from the L-Methionine Precursor
WO2015060649A1 (en) * 2013-10-23 2015-04-30 씨제이제일제당 (주) Microorganism producing o-succinyl homoserine and method for producing o-succinyl homoserine by using same
WO2016179545A1 (en) * 2015-05-06 2016-11-10 Trelys, Inc. Compositions and methods for biological production of methionine

Non-Patent Citations (2)

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Title
DATABASE Protein 4 March 2015 (2015-03-04), ANONYMOUS: "homoserine O-acetyltransferase [Corynebacterium glutamicum]", XP055584458, retrieved from NCBI Database accession no. WP_011013793.1 *
MORDUKHOVA, ELENA A.: "Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone", BMC MICROBIOLOGY, vol. 13, no. 179, 30 July 2013 (2013-07-30), pages 1 - 13, XP021157782 *

Also Published As

Publication number Publication date
US20200224228A1 (en) 2020-07-16
JP2020525018A (en) 2020-08-27
JP6946479B2 (en) 2021-10-06
US10982245B2 (en) 2021-04-20
WO2019004780A2 (en) 2019-01-03
EP3647415A4 (en) 2021-02-24
EP3647415A2 (en) 2020-05-06
RU2747494C1 (en) 2021-05-05
CN111032862A (en) 2020-04-17
SG11201913899UA (en) 2020-01-30
KR20190003402A (en) 2019-01-09
KR102112208B1 (en) 2020-05-19
CN111032862B (en) 2023-09-19
BR112019028095B1 (en) 2023-03-21
BR112019028095A2 (en) 2020-07-14
EP3647415B1 (en) 2025-12-03

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