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WO2019079912A1 - Procédé de lutte contre des champignons et des bactéries dans des carcasses et anatomie pathologique ; procédé pour la désinfection et l'aseptisation de surfaces et/ou d'environnements contaminés ; et utilisations - Google Patents

Procédé de lutte contre des champignons et des bactéries dans des carcasses et anatomie pathologique ; procédé pour la désinfection et l'aseptisation de surfaces et/ou d'environnements contaminés ; et utilisations

Info

Publication number
WO2019079912A1
WO2019079912A1 PCT/CL2018/050103 CL2018050103W WO2019079912A1 WO 2019079912 A1 WO2019079912 A1 WO 2019079912A1 CL 2018050103 W CL2018050103 W CL 2018050103W WO 2019079912 A1 WO2019079912 A1 WO 2019079912A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
fungi
bacteria
copper
bones
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CL2018/050103
Other languages
English (en)
Spanish (es)
Inventor
Donald MAC-LEAN HAEUSSLER
Patricio Ignacio RENDIC MUNIZAGA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MINERA SAN GERONIMO Cia
Original Assignee
MINERA SAN GERONIMO Cia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MINERA SAN GERONIMO Cia filed Critical MINERA SAN GERONIMO Cia
Publication of WO2019079912A1 publication Critical patent/WO2019079912A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/20—Copper

Definitions

  • the present invention refers to compositions based on copper salts that have a fungicidal and bactericidal effect to be applied to surfaces and environments for disinfection and sanitization.
  • pathological and forensic anatomy units since the risks associated with biological contaminants are known, among which bacteria and fungi stand out. The latter are very frequent, including their discovery in bones, which constitutes a risk associated both with health professionals working there, as well as with the judicial and forensic field due to the deterioration of the samples that are going to be subjected to various analyzes.
  • the present invention provides a solution capable of inhibiting the growth of fungi and bacteria in units of pathological and forensic anatomy, using doses low enough to have no adverse effects on the DNA of the samples to be studied.
  • the invention also relates to the use of these fungicidal and bactericidal compositions containing copper to be applied on biological material or on surfaces and environments contaminated with pathogens.
  • Figure 1 Diffractogram of a composition according to the present invention.
  • Figure 2 Infrared spectrum of a composition according to the present invention.
  • Figure 3 Infrared spectrum of a composition according to the present invention.
  • Figure 4 Graph of% growth inhibition of Penicillium sp.
  • copper salt in some of the compositions of the invention may include one or more of the following monovalent or, preferably, divalent copper salts, including the salts of copper sulfate, copper sulfate monohydrate, sulfate copper dihydrate, copper sulfate trihydrate, copper sulphate pentahydrate, (Cu) i 5 (S04) 4 (OH) 22 6 (H 2 0) (rambsbeckite), copper sulfate tetrahydroxide ((Cu 2+ ) 3 S0 4 (OH) 4 ), double ammonium sulfate and copper hexahydrate ((NH 4 ) 2 (Cu (S0 4 ) x6H 2 0), copper chloride, copper nitrate, copper bicarbonate, copper oxychloride, copper borate, copper sulfamate, copper oxychloride sulfate, and other different types of copper sulfate hydroxides, such as (Cu) 4S04 (
  • copper compounds which are suitable for the composition of the invention are copper hydroxide, cuprous oxide and cupric oxide
  • double salt in some of the compositions of the invention includes the salts of Tutton which possess the general formula M 2 M (S0 4 ) 2 (H 2 0) 6 wherein the monovalent cation may include K, Rb, Cs, and especially ammonium (NH 4 + ).
  • the divalent cation may include Mg, V, Cr, Mn, Fe, Co, Ni Zn, Cd and preferably Cu.
  • Copper salts that are preferably used for solid formulations are copper salts that are largely insoluble in water, such as copper oxychloride or copper hydroxide.
  • Copper salts that are preferably used for liquid or dispersed formulations are water soluble copper salts, such as copper sulfate pentahydrate.
  • the present invention relates to a fungicidal and bactericidal composition
  • a fungicidal and bactericidal composition comprising:
  • the fungicidal and bactericidal composition further comprises copper sulfate trihydrate (CuS0 4 x3H 2 0).
  • the fungicidal and bactericidal composition further comprises copper sulfate tetrahydroxide (Cu 3 S0 4 (OH) 4 ).
  • the fungicidal and bactericidal composition further comprises amorphous material.
  • the composition may also include lower amounts Ramsbeckite (Cu) i5 (S0 4 ) 4 (OH) 22 x6 (H 2 0).
  • the double salt of ammonium sulfate and copper hexahydrate ((NH4) 2 Cu (S0 4 ) 2 6H 2 0) is in an amount between 20 and 40% by weight to the total composition.
  • the copper sulfate pentahydrate salt (CuS0 4 x 5H 2 0) is present in an amount between 10 and 30% by weight with respect to the total composition.
  • copper sulfate trihydrate (CuS0 4 x3H 2 0) is in an amount between 5 and 30% by weight with respect to the total composition.
  • copper sulfate tetrahydroxide (Cu 3 S0 4 (OH) 4) is present in an amount between 2 and 15% by weight with respect to the total composition.
  • the amorphous material in another preferred embodiment of the invention in the fungicidal and bactericidal composition is in an amount between 5 and 30% by weight with respect to the total composition.
  • the fungicidal and bactericidal composition has a solubility in water, measured at 20 ° C, greater than 95% for a solution of 60 g of composition in 100 L of water.
  • the fungicidal and bactericidal composition has a solubility in water, measured at 20 ° C, greater than 93% for a solution of 120 g of composition in 100 L of water.
  • the present invention also relates to a method for the control of fungi and bacteria in bones and pathological anatomy, which comprises applying the composition described above, in all its modalities, on the surface to be analyzed.
  • the composition in a preferred embodiment of the invention in the method for the control of fungi and bacteria in bones and pathological anatomy, is applied diluted in water. In a still more preferred embodiment of the invention in the method for the control of fungi and bacteria in bones and pathological anatomy, the diluted composition is applied by spraying on the surface to be disinfected.
  • the present invention also relates to a method for disinfecting and sanitizing environments and / or surfaces contaminated with fungi and / or bacteria, which comprises applying the composition described above, in all its modalities, on the surface to be sanitized.
  • the composition in the method for disinfecting and sanitizing environments and / or surfaces contaminated with fungi and / or bacteria, is applied diluted in water.
  • the diluted composition is applied by spraying on the surface to be sanitized.
  • the present invention is also directed to the use of the fungicidal and bactericidal composition of the composition described above, in all its modalities, to control fungi and bacteria in bones and pathological anatomy.
  • the present invention is directed to the use of the fungicidal and bactericidal composition of the composition described above, in all its modalities, to disinfect and sanitize environments and / or surfaces contaminated with fungi and / or bacteria.
  • X-ray diffraction is a technique in which a beam of X-rays, at a certain wavelength, is interacted with a crystalline substance. This methodology allows to identify the crystalline phase both in its qualitative and quantitative aspect, since every crystalline phase presents a fingerprint that is its diffraction diagram. Results obtained
  • the samples of the composition have characteristic diffractograms of crystalline substances.
  • Table 1 shows the identification of the crystalline phases found in the sample of a preferred composition according to the present invention.
  • Figure 1 shows the diffractogram of a sample of the composition, in which each of the crystalline phases found in a composition according to the invention are identified.
  • This method consists of crossing the sample at a certain wavelength with an infrared beam of light and determining the qualitative composition of a substance through absorbance.
  • Each substance generates a unique absorbance curve for certain wavelengths.
  • Figures 2 and 3 show the spectra obtained for a composition according to the invention compared with cupric sulfate and cupric sulfate pentahydrate, respectively.
  • Table 2 determines the percentage of solubility of the composition according to the invention in water at different pH values.
  • Example 1 Evaluation of the effectiveness of the composition according to the invention in its application for decontamination of bones in forensic units and pathological anatomy It is proposed to perform the efficacy tests in a composition of the invention based on copper salts in bones with high concentrations of fungal contamination.
  • Mushroom sampling was carried out in a dependency of the Department of Legal Medicine of the Faculty of Medicine, University of Chile. Air samples of 50 liters were considered in 4 points of the room and a sample of 50 liters from the outside corridor. Samples were taken with an Air IDEAL® Biomerieux plate air impactor.
  • the characteristic reference values for residential areas fluctuate between 10 to 490 CFU / m, depending on the season and conditions of the property. In open spaces the fungal load can vary between 150 and 250 CFU / m. So the area of bones is considered highly contaminated and high risk to health.
  • Table 3 shows the results obtained, indicating the fungal load for each of the species identified in the sampling room and the corridor area. Due to the saturation of the instrument, these values are only referential and should not be considered as absolute values. The genus Penicillium predominated in all the samples.
  • test conditions were carried out under controlled conditions that attempt to simulate the application of the compound in aerosol form on biological material contaminated with fungal agents. Test conditions:
  • White biological material conidia of Penicillium sp. (Corresponds to the most represented fungal agent in the sample of the unit identified as "area of bone remains" of the Department of Legal Medicine of the Faculty of Medicine, University of Chile).
  • Culture medium Sabouraud agar.
  • White inoculum each plate of medium was inoculated with 195,000 conidia, in line with the high reported fungal load.
  • each test was carried out in a closed chamber, with a volume of 24 liters, containing three plates of Sabouraud agar, previously inoculated with the conidia of
  • Penicillium sp The tests consisted of the application in aerosol form of a certain volume of the composition according to the invention at different concentrations.
  • Reading of results the effects of each treatment were evaluated after three days of incubation at room temperature. For each test, the growth area of the cultures was determined. The results are expressed as the percentage of inhibition of growth relative to the control without treating with the composition according to the invention.
  • Table 4 specifies the details of the trial.
  • the analyzed samples correspond to samples by contact with biological material or inert surfaces and only fungi were isolated, not showing bacterial development, at 96 hours of incubation and proceeds to isolate characteristic colonies of:
  • Ciado sporium Ciado sporium.
  • composition according to the invention were made at concentrations of 3.8% w / v, 9.6% w / v, 19.2% w / v and 38.4% w / v, and 2 trials were implemented:
  • Mucor spp Table 7 Direct application composition according to the invention at a concentration of 38.4% w / v
  • Table 8 Application to walls composition according to the invention at a concentration of 3.8% w / v
  • composition according to the invention proves to be effective for the control of fungal growth.
  • composition according to the invention in high concentration (15.2% w / v) is effective as a fungicide under the conditions tested.
  • composition according to the invention For the concentrations of fungi studied, repeated doses of the composition according to the invention are required every 72 hours.
  • Example 2 Determination of the effectiveness as a fungicide of the composition according to the present invention
  • test conditions White biological material: conidia of Penicillium sp. (corresponding to the fungal agent most represented in the sample of the unit identified as "bone remains area" of the Department of Legal Medicine of the Faculty of Medicine, University of Chile). Culture medium: Sabouraud agar.
  • each test was carried out in a closed chamber, with a volume of 24 liters, containing three plates of Sabouraud agar, previously inoculated with the conidia of Penicillium sp.
  • the tests consisted of the application in aerosol form of a certain volume of a diluted solution of the composition at different concentrations. Table 10 details the details of each trial.
  • Test 1 1.0 5.0
  • composition according to the invention provides an effective solution for the control of fungi and bacteria in bones and pathological anatomy with doses high enough to effectively control fungi and bacteria, and at the same time low enough so as not to have effects on the DNA of the decontaminated material. , which is particularly due to the efficiency of the composition of the invention.
  • composition developed allows its use in disinfection and industrial sanitization through fungicide and bactericidal effect in process rooms, fruit packing rooms, factories, warehouses, etc.
  • composition of the present invention provides an effective solution in, for example, disinfection, decontamination and sanitization by bactericidal fungicidal effect in catastrophe management, hospitals, livestock industry, salmon industry, agricultural industry in general and for the food industry.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne un procédé pour lutter contre la présence de champignons et de bactéries dans des carcasses et l'anatomie pathologique sans endommager les prélèvements en vue de l'analyse de l'ADN. Ledit procédé consiste à appliquer une solution d'une composition comprenant des sels de cuivre, principalement un sel double de sulfate d'ammonium et du cuivre hexahydraté ((NH4)2Cu(SO4)2X6H2O) et un sel de sulfate de cuivre pentahydraté (CuSO4x5H2O). De même, l'invention concerne un procédé pour la désinfection et l'aseptisation de surfaces et/ou d'environnements contaminés par des champignons et/ou des bactéries par application de ladite composition.
PCT/CL2018/050103 2017-10-25 2018-10-25 Procédé de lutte contre des champignons et des bactéries dans des carcasses et anatomie pathologique ; procédé pour la désinfection et l'aseptisation de surfaces et/ou d'environnements contaminés ; et utilisations Ceased WO2019079912A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762577110P 2017-10-25 2017-10-25
US62/577,110 2017-10-25

Publications (1)

Publication Number Publication Date
WO2019079912A1 true WO2019079912A1 (fr) 2019-05-02

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/CL2018/050103 Ceased WO2019079912A1 (fr) 2017-10-25 2018-10-25 Procédé de lutte contre des champignons et des bactéries dans des carcasses et anatomie pathologique ; procédé pour la désinfection et l'aseptisation de surfaces et/ou d'environnements contaminés ; et utilisations
PCT/CL2018/050102 Ceased WO2019079911A1 (fr) 2017-10-25 2018-10-25 Composition fongicide et bactéricide comprenant des sels à base de cuivre ; procédé pour éviter et/ou traiter l'infestation de plantes utiles par des bactéries ou champignons ; et utilisation de ladite composition

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/CL2018/050102 Ceased WO2019079911A1 (fr) 2017-10-25 2018-10-25 Composition fongicide et bactéricide comprenant des sels à base de cuivre ; procédé pour éviter et/ou traiter l'infestation de plantes utiles par des bactéries ou champignons ; et utilisation de ladite composition

Country Status (3)

Country Link
AR (1) AR113795A1 (fr)
PE (1) PE20210476A1 (fr)
WO (2) WO2019079912A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060178431A1 (en) * 2004-12-21 2006-08-10 Hartfeldt Will H Chemotherapeutic agents for the control of plant and animal diseases
CL2013002173A1 (es) * 2013-07-30 2014-02-21 Compania Minera San Geronimo Formulacion agroquimica que comprende sulfato de cobre pentahidratoado soluble, cuso4 x 5h2o y sulfato de cobre alcalino insoluble; proceso de obtencion; y su uso para el tratamiento y prevencion de enfermedades causadas por hongos y bacterias.
US8951573B2 (en) * 2010-05-21 2015-02-10 Lion Corporation Disinfectant composition for hard articles, and method for disinfecting of surface of hard article
MX2015009708A (es) * 2013-02-01 2016-09-13 Compañia Minera San Geronimo Matriz impregnable de origen vegetal, animal o sintetica o mezclas de las mismas que contiene un compuesto antimicrobiano distribuido homogeneamente: procedimiento para impregnar un compuesto en dicha matriz; y su uso en la preparacion de elementos antmicrobianos.
US9474282B2 (en) * 2013-12-13 2016-10-25 Tony John Hall Acid-solubilized copper-ammonium complexes and copper-zinc-ammonium complexes, compositions, preparations, methods, and uses

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6923991B2 (en) * 2002-02-20 2005-08-02 Global Microbio Products, L.L.C. Bactericide solution and method of making same
US20170055534A1 (en) * 2015-08-25 2017-03-02 Phyton Corporation Process for treating plants to control bacterial and fungal growth

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060178431A1 (en) * 2004-12-21 2006-08-10 Hartfeldt Will H Chemotherapeutic agents for the control of plant and animal diseases
US8951573B2 (en) * 2010-05-21 2015-02-10 Lion Corporation Disinfectant composition for hard articles, and method for disinfecting of surface of hard article
MX2015009708A (es) * 2013-02-01 2016-09-13 Compañia Minera San Geronimo Matriz impregnable de origen vegetal, animal o sintetica o mezclas de las mismas que contiene un compuesto antimicrobiano distribuido homogeneamente: procedimiento para impregnar un compuesto en dicha matriz; y su uso en la preparacion de elementos antmicrobianos.
CL2013002173A1 (es) * 2013-07-30 2014-02-21 Compania Minera San Geronimo Formulacion agroquimica que comprende sulfato de cobre pentahidratoado soluble, cuso4 x 5h2o y sulfato de cobre alcalino insoluble; proceso de obtencion; y su uso para el tratamiento y prevencion de enfermedades causadas por hongos y bacterias.
US9474282B2 (en) * 2013-12-13 2016-10-25 Tony John Hall Acid-solubilized copper-ammonium complexes and copper-zinc-ammonium complexes, compositions, preparations, methods, and uses

Also Published As

Publication number Publication date
WO2019079911A1 (fr) 2019-05-02
AR113795A1 (es) 2020-06-10
PE20210476A1 (es) 2021-03-08

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