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WO2018237197A3 - Crispr-mediated selective gene disruption in plants - Google Patents

Crispr-mediated selective gene disruption in plants Download PDF

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Publication number
WO2018237197A3
WO2018237197A3 PCT/US2018/038839 US2018038839W WO2018237197A3 WO 2018237197 A3 WO2018237197 A3 WO 2018237197A3 US 2018038839 W US2018038839 W US 2018038839W WO 2018237197 A3 WO2018237197 A3 WO 2018237197A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
crispr
gene disruption
selective gene
mediated selective
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2018/038839
Other languages
French (fr)
Other versions
WO2018237197A2 (en
Inventor
Zengyu Wang
Jongjin Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Noble Research Institute LLC
Original Assignee
Noble Research Institute LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Noble Research Institute LLC filed Critical Noble Research Institute LLC
Publication of WO2018237197A2 publication Critical patent/WO2018237197A2/en
Publication of WO2018237197A3 publication Critical patent/WO2018237197A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR associated protein 9 (Cas9) offer an effective way of creating targeted mutagenesis in plants. To alleviate concerns related to genetically modified plants, the present disclosure provides a novel and efficient genome editing system for editing the genomes of complex plants. Specifically, methods for reducing lignin content in switchgrass are disclosed.
PCT/US2018/038839 2017-06-21 2018-06-21 CRISPR MEDIATED SELECTIVE GENE DISRUPTION IN PLANTS Ceased WO2018237197A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762523025P 2017-06-21 2017-06-21
US62/523,025 2017-06-21

Publications (2)

Publication Number Publication Date
WO2018237197A2 WO2018237197A2 (en) 2018-12-27
WO2018237197A3 true WO2018237197A3 (en) 2019-01-31

Family

ID=64737399

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/038839 Ceased WO2018237197A2 (en) 2017-06-21 2018-06-21 CRISPR MEDIATED SELECTIVE GENE DISRUPTION IN PLANTS

Country Status (1)

Country Link
WO (1) WO2018237197A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150082478A1 (en) * 2013-08-22 2015-03-19 E I Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150082478A1 (en) * 2013-08-22 2015-03-19 E I Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
XU ET AL.: "Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production", NEW PHYTOLOGIST, vol. 192, no. 3, November 2011 (2011-11-01), pages 611 - 625, XP055568360 *

Also Published As

Publication number Publication date
WO2018237197A2 (en) 2018-12-27

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