[go: up one dir, main page]

WO2018135842A1 - Souche de brevibacillus reuszeri ayant la capacité de prévenir la chute des cheveux, de favoriser la croissance des cheveux ou d'améliorer la fonction sexuelle, et composition la comprenant - Google Patents

Souche de brevibacillus reuszeri ayant la capacité de prévenir la chute des cheveux, de favoriser la croissance des cheveux ou d'améliorer la fonction sexuelle, et composition la comprenant Download PDF

Info

Publication number
WO2018135842A1
WO2018135842A1 PCT/KR2018/000756 KR2018000756W WO2018135842A1 WO 2018135842 A1 WO2018135842 A1 WO 2018135842A1 KR 2018000756 W KR2018000756 W KR 2018000756W WO 2018135842 A1 WO2018135842 A1 WO 2018135842A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
brevibacillus
strain
rugeri
ceb
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/000756
Other languages
English (en)
Korean (ko)
Inventor
염규진
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coenbio Co Ltd
Original Assignee
Coenbio Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coenbio Co Ltd filed Critical Coenbio Co Ltd
Publication of WO2018135842A1 publication Critical patent/WO2018135842A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Definitions

  • the present invention relates to a novel strain having a hair loss prevention, promoting hair growth or improving sexual function and a composition comprising the same.
  • the cause of hair loss is not well known yet, but there are reports of possible causes such as genetic factors, hormonal abnormalities, and immunological abnormalities.
  • the present invention is to provide a novel strain effective for preventing hair loss, promoting hair growth or improving sexual function.
  • the present invention is to provide a composition for preventing hair loss, promoting hair growth or improving sexual function that can be safely used without side effects.
  • One aspect of the invention provides a novel Brevibacillus rugeri Brevibacillus reuszeri KCCM11911P strain.
  • the morphological characteristics of the strain are Gram-positive bacteria, have a nucleotide sequence of SEQ ID NO: 1, and have 99% homology with Brevibacillus rugeri as determined by 16s rRNA partial sequencing. Accordingly, the inventors deposited the newly isolated Brevibacillus rugeri Ceb-ch-003 at the Korean Culture Center of Microorganisms (120-861, Hongje-2ga-gil, Seodaemun-gu, Seoul) on October 7, 2016. Deposited with KCCM11911P.
  • the strain is isolated from the traditional Korean food Cheonggukjang, a kind of aerobic Bacillus subtilis. The strain is effective in preventing hair loss, promoting hair growth, improving sexual function, lowering cholesterol, and / or lipolysis.
  • Another aspect of the invention relates to a culture of Brevibacillus rugeri KCCM11911P, concentrates thereof, or dried products thereof.
  • the novel isolated strain of the present invention can be cultured through a conventional method for culturing Bacillus or Brevibacillus genus.
  • the medium may be natural or synthetic medium.
  • the carbon source of the medium for example, glucose, sucrose, dextrin, glycerol, starch and the like can be used, and as the nitrogen source, peptone, meat extract, yeast extract, dried yeast, soybean, ammonium salt, nitrate and other organic or inorganic Nitrogen containing compounds may be used, but are not limited to these components.
  • Inorganic salts contained in the medium include magnesium. Manganese, calcium. Iron, potassium and the like can be used, but are not limited thereto.
  • culture temperature conditions of the newly isolated strain of the present application can be incubated for 12 hours to 4 days in the temperature range of 20 ⁇ 40 °C.
  • medicinal preparations such as seotae, baekshou, succinct, licorice, celery, tofu, cinnamon, donkey, Seokpo, red squid, ephedra, ginger, white porcelain, cheoncho, horseback riding, mantis, etc.
  • culturing by adding a mixture thereof, by using the culture it is possible to further improve the effects of preventing hair loss, promoting hair growth, and improving sexual function of the present strain.
  • it is preferable to add frosted and / or white sewage to the culture medium and the amount thereof to be added is preferably 0.01 to 10% by weight, specifically 0.01 to 5% by weight, based on the weight of the medium, respectively.
  • the culture of the newly isolated strain may be a culture stock solution including the cells, and may also be a culture cell from which the culture supernatant is removed or concentrated.
  • the culture composition may further include components necessary for culturing Bacillus or Brevibacillus strains, as well as components that act synergistically to the growth of Bacillus or Brevibacillus strains, the composition according to the conventional It can be easily selected by those skilled in the art.
  • the state of the strain may be a liquid state or dry state
  • the drying method is possible, but not limited to ventilation drying, natural drying, spray drying and freeze drying.
  • the present invention relates to a composition
  • a composition comprising a Brevibacillus rugeri strain, a culture thereof, a concentrate thereof, or a dried product thereof.
  • the Brevibacillus rugeri strain is specifically, Brevibacillus rugeri Brevibacillus reuszeri KCCM11911P strain.
  • the composition may be a food composition or a pharmaceutical composition for preventing hair loss, promoting hair growth, or improving sexual function, and further, has a lipolytic effect and / or a cholesterol lowering effect.
  • One aspect of the food composition may be a food, health food, dietary supplement, or health functional food composition.
  • One aspect of the pharmaceutical composition may be a quasi-drug or a pharmaceutical preparation.
  • the composition is Seoryae, Baekshou, Sukjihwang, licorice, Cheongung, tofu, cinnamon, Angelica, Seokchangpo, Red sesame, ephedra, prickly pear, pine needles, pine flower, celticus, jimo, ginger, white porcelain, cheoncho, horseback riding, bay
  • Herbal ingredients effective for preventing hair loss or promoting hair growth such as herbal preparations such as a typewriter, extracts thereof, or mixtures thereof; Or may further comprise a pharmaceutical component such as minoxidil or finasteride.
  • the composition is Lactobacillus , Lactococcus , Propionibacterium , Enterocuccus , Strains such as the Bifidobacterium genus may be further included.
  • Enterococcus faecium e.g. KCCM11909P
  • Lactobacillus fermentum E.g. KCCM11910P
  • Enterococcus faecium e.g., KCCM11909P
  • Leuconostoc mesenteroides e.g. KCCM11827P
  • Lactobacillus sakei e.g. KCCM11841P
  • cultures thereof or mixtures thereof.
  • Lactobacillus fermentum Eg KCCM11910P
  • Leukonostock Mesenteroides KCCM11827P Lactobacillus sakei KCCM11841P, or cultures thereof, or mixtures thereof.
  • the composition may further comprise collagen.
  • the composition of the present invention comprises 5 x 10 4 to 5 x 10 10 CFU / ml Brevibacillus rugeri, specifically KCCM11911P strain at a concentration of 5 x 10 4 to 5 x 10 8 CFU / ml, preferably Comprises 1 ⁇ 10 6 to 1 ⁇ 10 9 CFU / ml of Brevibacillus rugeri (eg KCCM11911P).
  • An amount of 30 ml to 100 ml per dose of the strain composition may be administered to the subject once to four times a day.
  • composition of the present invention may further include a pharmaceutically or food acceptable carrier, and may be formulated with the carrier to be provided as a food or medicine.
  • the term "pharmaceutically or food acceptable carrier” refers to a carrier or diluent that does not stimulate the organism and does not inhibit the biological activity and properties of the administered strain.
  • composition may be formulated in a variety of oral or parenteral formulations, but may preferably be formulated in a liquid oral solution.
  • acceptable pharmaceutical or food acceptable carriers are sterile and physiologically compatible carriers, including saline, sterile water, buffered saline, albumin injectable solutions, dextrose solution, maltodextrin solution, glycerol and these
  • saline sterile and physiologically compatible carriers
  • sterile water sterile water
  • buffered saline albumin injectable solutions
  • dextrose solution dextrose solution
  • maltodextrin solution glycerol
  • glycerol glycerol
  • diluents, dispersants, surfactants, binders and lubricants may be additionally added to formulate into liquid formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.
  • binders, emulsifiers, preservatives, and the like may be further added to prevent degradation of the composition, or amino acids, vitamins, enzymes, flavoring agents, nonprotein nitrogen compounds, silicates, buffers, extractants, Oligosaccharides and the like may be further included.
  • the dosage is 5 x 10 4 to 5 x 10 8 CFU / ml Brevibacillus rugeri (e.g. KCCM11911P) strain, preferably 1 x 10 6 to 1 x 10 8 CFU / ml
  • the amount of Brevibacillus rugeri e.g., KCCM11911P
  • 50 ml per dose can be administered 2 to 4 times per day in an amount of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Brevibacillus rugeri (eg KCCM11911P). More specifically, the amount of Brevibacillus rugeri (eg, KCCM11911P) of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml may be administered once daily to 50ml to 100ml once or twice. More specifically, 1 x 10 6 to 1 x 10 8 CFU / ml Brevibacillus rugeri (e.g. KCCM11911P) is administered 50ml to 100ml once 30 minutes before breakfast, 50ml before dinner 30 minutes or before bedtime To 100 ml once.
  • 1 x 10 6 to 1 x 10 8 CFU / ml Brevibacillus rugeri e.g. KCCM11911P
  • Formulations for oral administration comprising the Brevibacillus rugeri of the present invention (eg KCCM11911P) include, for example, tablets, troches, lozenges, water-soluble or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules. , Syrup or elixirs.
  • binders such as cellulose or gelatin
  • excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, stearic acid Lubricants such as magnesium, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax may be included, and the capsule formulation may further contain a liquid carrier such as fatty oil in addition to the aforementioned substances.
  • the daily dose of the composition or the Brevibacillus rugeri is dependent on the patient's weight, age, sex, health condition, treatment, prevention, or major symptoms, time of administration, method of administration and severity. Can vary from about 0.0001 mg / kg to about 10 g / kg, specifically about 0.01 mg / kg to about 500 mg / kg, once to six times daily, for example once to four times daily, or Applicable
  • Brevibacillus rugeri in the composition may be liquid or dry, preferably in liquid form. Drying methods may be, but not limited to, ventilation drying, natural drying, spray drying and freeze drying. Brevibacillus rugeri of the present invention (eg KCCM11911P) is in the form of a powder in the amount of 0.05 to 80% by weight, for example 0.1 to 70% by weight, 0.1 to 50% by weight, or 1 to 30% by weight of the composition Can be mixed.
  • One aspect of the present invention relates to a composition for preventing hair loss or promoting hair growth, comprising a Brevibacillus rugeri (eg, KCCM11911P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • a composition for preventing hair loss or promoting hair growth comprising a Brevibacillus rugeri (eg, KCCM11911P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • another aspect of the invention comprises administering to a subject a therapeutic or prophylactically effective amount of a Brevibacillus rugeri (eg, KCCM11911P) strain or culture thereof, concentrate or dried product thereof to prevent hair loss or promote hair growth It relates to a method for preventing hair loss or a method for treating hair loss.
  • the therapeutic or prophylactically effective amount may vary depending on the weight, age, sex, health condition, treatment, prevention, or major symptoms, time of administration, administration method, and severity of the patient, as described above.
  • a Brevibacillus rugeri of 5 ⁇ 10 4 to 5 ⁇ 10 8 CFU / ml (eg KCCM11911P) strain preferably 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Brevibacillus rugeri (eg : KCCM11911P) may be administered from 30ml to 100ml once to 4 times per day.
  • 50 ml per dose can be administered 2 to 4 times per day in an amount of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Brevibacillus rugeri (eg KCCM11911P).
  • the amount of Brevibacillus rugeri (eg, KCCM11911P) of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml may be administered once to twice daily with 50ml to 100ml per dose. More specifically, 1 x 10 6 to 1 x 10 8 CFU / ml Brevibacillus rugeri (e.g. KCCM11911P) is administered 50ml to 100ml once 30 minutes before breakfast, 50ml before dinner 30 minutes or before bedtime To 100 ml once.
  • KCCM11911P is administered 50ml to 100ml once 30 minutes before breakfast, 50ml before dinner 30 minutes or before bedtime To 100 ml once.
  • One aspect of the invention relates to a composition for improving sexual dysfunction, comprising Brevibacillus rugeri (eg KCCM11911P) strain or culture thereof, concentrate thereof or dried product thereof.
  • the sexual dysfunction improvement may be, for example, an improvement in erectile dysfunction.
  • another aspect of the invention comprises administering to a subject a therapeutic or prophylactically effective amount of a Brevibacillus rugeri (eg, KCCM11911P) strain or culture thereof, concentrate or dried product thereof, for the prevention or treatment of sexual dysfunction.
  • the therapeutic or prophylactically effective amount is the same as described above.
  • One aspect of the present invention relates to a composition for lowering cholesterol, comprising a Brevibacillus rugeri (eg, KCCM11911P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • a composition for lowering cholesterol comprising a Brevibacillus rugeri (eg, KCCM11911P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • administering to a subject a therapeutic or prophylactically effective amount of a Brevibacillus rugeri (e.g., KCCM11911P) strain or culture thereof, concentrate or dried product thereof for lowering cholesterol It relates to a method for preventing or treating cholesterol.
  • the therapeutic or prophylactically effective amount is the same as described above.
  • One aspect of the present invention relates to a composition for lipolysis, comprising a Brevibacillus rugeri (eg, KCCM11911P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • another aspect of the invention comprises administering to a subject for the prevention or treatment of hyperlipidemia a therapeutic or prophylactically effective amount of a Brevibacillus rugeri (eg, KCCM11911P) strain or culture thereof, concentrate thereof or dried product thereof.
  • a method for preventing or treating hyperlipidemia is the same as described above.
  • Brevibacillus rugeri eg KCCM11911P
  • KCCM11911P Brevibacillus rugeri
  • a composition comprising the same is effective for preventing hair loss, promoting hair growth or improving sexual function.
  • Brevibacillus rugeri eg KCCM11911P
  • a composition comprising the same can be used to prevent hair loss, promote hair growth, lower cholesterol or improve sexual function safely without side effects.
  • Figure 1 is a comparison of hair before and after taking Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) strain (about 1 month).
  • Figure 2 is a comparison of hair before and after taking Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) strain (about 1.5 months).
  • Figure 3 is a comparison of the hair before and after taking the Brevibacillus Rugeri Ceb-ch-003 (KCCM11911P) strain (about 3 months).
  • Figure 4 is a comparison of hair before and after taking Brevibacillus Rugeri Ceb-ch-003 (KCCM11911P) and Lactobacillus permanent Ceb-ch-002 (KCCM11910P).
  • FIG. 5 is a comparison of hair before and after taking Brevibacillus lugeri Ceb-ch-003 (KCCM11911P) and Lactobacillus permanent Ceb-ch-002 (KCCM11910P).
  • FIG. 6 is a comparison of hair before and after taking Brevibacillus lugeri Ceb-ch-003 (KCCM11911P) and Lactobacillus permanent Ceb-ch-002 (KCCM11910P).
  • FIG. 7 is a graph showing the NO production concentration compared to the Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) strain and other lactic acid strain Lactobacillus plantarum .
  • FIG. 8 is a graph showing the cholesterol lowering effect of the Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) strain and other lactic acid strain Lactobacillus plantarum .
  • Cheonggukjang a traditional Korean food, was collected, and the obtained sample was diluted in stages and incubated in BHI solid medium (Difco, USA) added with 3% sodium chloride for 24 hours at 37 ° C. The predominant strain was isolated from the sample. Selected colonies were purely separated by culturing in a new medium over 3 times, and the pure cultured cells were stored in a medium to which 20% glycerol was added and stored at below 70 ° C.
  • morphological and biochemical investigations were performed primarily.
  • the morphological features were Gram-stained and Gram-positive.
  • 16s rRNA partial sequencing was performed, and as a result, the nucleotide sequence of SEQ ID NO: 1, and the isolated strain was a type of aerobic Bacillus subtilis having 99% homology with Brevibacillus rugeri. It was confirmed to be.
  • the inventors deposited the newly isolated Brevibacillus rugeri Ceb-ch-003 at the Korean Culture Center of Microorganisms (120-861, Hongje-2ga-gil, Seodaemun-gu, Seoul) on October 7, 2016. Deposited with KCCM11911P.
  • strain powder The strain was lyophilized to prepare strain powder.
  • Ceb-ch-003 Inoculated with 0.2 to 0.4 liters in a sterile state and then incubated for 2 to 3 days at 35 ° C to obtain a culture solution of Brevibacillus Rugeri Ceb-ch-003.
  • Example 1 A breather ratio Bacillus Lu Jerry Ceb-ch-003 (KCCM11911P) 56 in the depilation symptoms strain culture aged, for a total of three male of 45 years old and 56 years of age and one month to two months obtained from 1 x 10 6
  • An amount of Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) to 1 ⁇ 10 8 CFU / ml was orally administered daily between 50 ml and 100 ml twice daily in the morning and evening.
  • the number of hairs falling out after hair washing once at 10-day intervals before and after administration of the strain was 10 days, 20 days and 30 days.
  • Hair loss number before and after taking Test subject Taking date 56 year old male 45 year old male 56 year old male Before taking 70 hair loss 49 hair loss 44 hair loss 10 days after taking 61 hair loss 40 hair loss 36 hair loss 20 days after taking 49 hair loss 29 hair loss 25 hair loss 30 days after taking 40 hair loss 19 hair loss 12 hair loss
  • the Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) strain of the present invention is effective in preventing hair loss, and is also effective in treating hair loss by remarkably improving hair thickness, quantity and shine.
  • Example 3 Brevibacillus Rugeri Ceb -ch-003 ( KCCM11911P ) Strains Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Hair loss prevention effect of strain combination
  • the culture solution of Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) strain obtained in Example 1 was isolated from Cheonggukjang and Korean Culture Center of Microorganisms, Hongje-2, Seodaemun-gu, Seoul, October 7, 2016.
  • a culture solution of Lactobacillus fermentum KCCM11910P strain deposited with accession number KCCM11910P to Ga-gil 120-861) was administered to a total of three males 55, 53 and 48 years old with alopecia for 1 month to 2 months. It was.
  • Specific dosing method is 1 x 10 6 to 1 x 10 8 CFU / ml Brevibacillus Rugeri Ceb-ch-003 (KCCM11911P) of 25ml to 50ml, 1 x 10 6 to 1 x 10 8 CFU / ml Lactobacillus fermentum KCCM11910P strain 25 ml to 50 ml per dose were administered orally twice daily.
  • Hair loss number before and after taking Test subject Taking date 55 year old male 53 year old male 48 year old male Before taking 60 hair loss 55 hair loss 57 hair loss 10 days after taking 45 hair loss 43 hair loss 45 hair loss 20 days after taking 23 hair loss 27 hair loss 36 hair loss 30 days after taking 10 hair loss 7 hair loss 13 hair loss
  • the Brevibacillus Rugeri Ceb-ch-003 (KCCM11911P) strains of the present invention when combined with other strains, Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strains, it is more effective in preventing hair loss. Can be.
  • KCCM11911P Brevibacillus rugeri Ceb-ch-003
  • KCCM11911P lyophilized powder of Brevibacillus rugeri Ceb-ch-003
  • Lactobacillus plantarum powder solution of 1 mg / ml to 5 mg / ml was used as a liquid and a control, respectively.
  • the cholesterol removal ability defined in the present invention can be specifically measured according to the following method:
  • the Brevibacillus rugeri Ceb-ch-003 (KCCM11911P) strain powder of the present invention is effective in lowering cholesterol in proportion to the concentration, which is significantly improved compared to Lactobacillus plantarum , a lactic acid bacterium. .
  • Example 5 Brevibacillus Rugeri Ceb -ch-003 ( KCCM11911P ) Improvement of sexual function of strain And prevention effect
  • Lactobacillus plantarum 1 ⁇ g / ml 4.1 ( ⁇ M) 2.6 ( ⁇ M) 10 ⁇ g / ml 4.5 ( ⁇ M) 2.9 ( ⁇ M) 20 ⁇ g / ml 4.9 ( ⁇ M) 3.2 ( ⁇ M) 30 ⁇ g / ml 5.4 ( ⁇ M) 3.5 ( ⁇ M) 40 ⁇ g / ml 6.4 ( ⁇ M) 3.8 ( ⁇ M) 50 ⁇ g / ml 7.3 ( ⁇ M) 4.1 ( ⁇ M)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une nouvelle souche ayant la capacité de prévenir la perte des cheveux, de favoriser la croissance des cheveux ou d'améliorer la fonction sexuelle, et une composition la comprenant.
PCT/KR2018/000756 2017-01-19 2018-01-16 Souche de brevibacillus reuszeri ayant la capacité de prévenir la chute des cheveux, de favoriser la croissance des cheveux ou d'améliorer la fonction sexuelle, et composition la comprenant Ceased WO2018135842A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0009280 2017-01-19
KR1020170009280A KR101794771B1 (ko) 2017-01-19 2017-01-19 탈모 예방, 발모 촉진 또는 성기능 개선능이 있는 브레비바실러스 루제리 균주 및 이를 포함하는 조성물

Publications (1)

Publication Number Publication Date
WO2018135842A1 true WO2018135842A1 (fr) 2018-07-26

Family

ID=60385892

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/000756 Ceased WO2018135842A1 (fr) 2017-01-19 2018-01-16 Souche de brevibacillus reuszeri ayant la capacité de prévenir la chute des cheveux, de favoriser la croissance des cheveux ou d'améliorer la fonction sexuelle, et composition la comprenant

Country Status (2)

Country Link
KR (1) KR101794771B1 (fr)
WO (1) WO2018135842A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101794771B1 (ko) * 2017-01-19 2017-11-09 (주)코엔바이오 탈모 예방, 발모 촉진 또는 성기능 개선능이 있는 브레비바실러스 루제리 균주 및 이를 포함하는 조성물
KR102254553B1 (ko) * 2019-11-08 2021-05-21 주식회사 에치와이 모발 성장 촉진 효능을 가지는 락토바실러스 파라카제이 hy7015 및 이를 유효성분으로 함유하는 모발 성장 촉진을 위한 조성물

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130069019A (ko) * 2011-12-16 2013-06-26 부산대학교 산학협력단 발효된 맥문동 추출물을 포함하는 발모 촉진제 및 그 제조방법
KR20130071956A (ko) * 2011-12-21 2013-07-01 박정훈 고온성 미생물을 이용하여 유기성 폐기물로부터 퇴비를 제조하는 방법
EP2765185A2 (fr) * 2013-02-11 2014-08-13 SC Euro Bio SRL Souche de Brevibacillus parabrevis et composition à libération contrôlée à base de celle-ci
KR101452320B1 (ko) * 2014-03-11 2014-10-22 주식회사 벤스랩 전립선 비대증 및 탈모를 개선하는 ５―알파 환원효소 억제용 발효 동백오일
KR20160050716A (ko) * 2014-10-30 2016-05-11 대한민국(농촌진흥청장) 브레비바실러스 속 R2a23 및 이의 용도
KR101794771B1 (ko) * 2017-01-19 2017-11-09 (주)코엔바이오 탈모 예방, 발모 촉진 또는 성기능 개선능이 있는 브레비바실러스 루제리 균주 및 이를 포함하는 조성물

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101058245B1 (ko) 2008-11-03 2011-08-22 부경대학교 산학협력단 생선폐기물을 이용하여 액체비료를 제조하는 방법

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130069019A (ko) * 2011-12-16 2013-06-26 부산대학교 산학협력단 발효된 맥문동 추출물을 포함하는 발모 촉진제 및 그 제조방법
KR20130071956A (ko) * 2011-12-21 2013-07-01 박정훈 고온성 미생물을 이용하여 유기성 폐기물로부터 퇴비를 제조하는 방법
EP2765185A2 (fr) * 2013-02-11 2014-08-13 SC Euro Bio SRL Souche de Brevibacillus parabrevis et composition à libération contrôlée à base de celle-ci
KR101452320B1 (ko) * 2014-03-11 2014-10-22 주식회사 벤스랩 전립선 비대증 및 탈모를 개선하는 ５―알파 환원효소 억제용 발효 동백오일
KR20160050716A (ko) * 2014-10-30 2016-05-11 대한민국(농촌진흥청장) 브레비바실러스 속 R2a23 및 이의 용도
KR101794771B1 (ko) * 2017-01-19 2017-11-09 (주)코엔바이오 탈모 예방, 발모 촉진 또는 성기능 개선능이 있는 브레비바실러스 루제리 균주 및 이를 포함하는 조성물

Also Published As

Publication number Publication date
KR101794771B1 (ko) 2017-11-09

Similar Documents

Publication Publication Date Title
WO2018135843A2 (fr) Souche de lactobacillus fermentum pouvant empêcher la perte de cheveux, favoriser la pousse des cheveux, ou améliorer la fonction sexuelle, et composition comprenant cette souche
WO2018043864A1 (fr) Souche de leuconostoc holzapfelii ayant pour effets de prévenir la perte des cheveux, de favoriser la pousse des cheveux ou d'améliorer la fonction sexuelle, et composition la comprenant
US7785581B2 (en) Composition and method for reducing feces toxins and treating digestive disorders
WO2018043874A1 (fr) Composition permettant de prévenir la chute des cheveux ou de favoriser la pousse des cheveux, comprenant des souches présentant un effet de lipolyse
WO2023038350A1 (fr) Composition pour soulager le syndrome prémenstruel, contenant des souches de lactobacillus mélangées en tant que principe actif
WO2022086041A1 (fr) Nouvelle souche de lactobacillus sp. et son utilisation
WO2023038418A1 (fr) Souche de bifidobacterium bifidum eps da-laim pour le maintien de la santé intestinale, possédant l'effet de favoriser la croissance des lactobacilles, et polysaccharides qui en sont issus
WO2013176327A1 (fr) Composition pharmaceutique et aliment fonctionnel de santé pour la prévention ou le traitement de maladies osseuses, contenant un produit de fermentation de bactéries lactiques de soupe détox de coptis chinensis en tant que principe actif
WO2024172497A1 (fr) Souche de lacticaseibacillus rhamnosus et utilisations d'amélioration de l'immunité associées
WO2020045972A1 (fr) Souche de lactobacillus fermentum mg4231 ou de lactobacillus fermentum mg4244 dérivé du corps humain, ayant une activité anti-obésité, et composition la comprenant
KR101840376B1 (ko) 탈모 예방, 발모 촉진 또는 성기능 개선능이 있는 락토바실러스 퍼멘텀 균주 및 이를 포함하는 조성물
WO2018135842A1 (fr) Souche de brevibacillus reuszeri ayant la capacité de prévenir la chute des cheveux, de favoriser la croissance des cheveux ou d'améliorer la fonction sexuelle, et composition la comprenant
WO2021194011A1 (fr) Probiotiques pour prévenir ou traiter la maladie d'alzheimer, et composition alimentaire les comprenant pour prévenir ou traiter une maladie buccale
KR102004346B1 (ko) 지방 분해능을 갖는 균주를 포함하는 탈모 방지 또는 발모 촉진용 조성물
WO2023177218A1 (fr) Souche de lacticaseibacillus paracasei ayant une activité inhibitrice de pathogène parodontal, et son utilisation
KR101790548B1 (ko) 지방 분해능을 갖는 균주를 포함하는 탈모 방지 또는 발모 촉진용 조성물
WO2023075357A1 (fr) Souche de lactobacillus crispatus et composition pour la prévention ou le traitement de vaginite la comprenant
WO2023038258A1 (fr) Nouvelle souche lactobacillus gasseri lm1065 issue du lait maternel, et composition pour soulager le syndrome prémenstruel comprenant ladite souche ou son produit de culture
WO2025009766A1 (fr) Souche de bifidobacterium animalis subsp. lactis et son utilisation pour améliorer l'état des cheveux ou du cuir chevelu
TWI810852B (zh) 凝結芽孢桿菌bc198或其代謝產物用於預防或輔助治療化療導致腸道受損相關病變或菌叢失衡之用途
WO2023177215A1 (fr) Souche de weissella cibaria ayant une activité d'amélioration de la santé dentaire et son utilisation
WO2019245225A1 (fr) Composition permettant d'améliorer la fonction intestinale comprenant une souche leuconostoc sp.
WO2023229104A1 (fr) Composition de complexe probiotique ayant des fonctions immunomodulatrices et d'homéostasie immunitaire
KR101882303B1 (ko) 지방 분해능을 갖는 균주를 포함하는 탈모 방지 또는 발모 촉진용 조성물
KR20180085668A (ko) 탈모 예방, 발모 촉진 또는 성기능 개선능이 있는 브레비바실러스 루제리 균주 및 이를 포함하는 조성물

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18742258

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18742258

Country of ref document: EP

Kind code of ref document: A1