[go: up one dir, main page]

WO2018129486A3 - Composition et procédés pour une expression améliorée de gène rapporteur knock-in - Google Patents

Composition et procédés pour une expression améliorée de gène rapporteur knock-in Download PDF

Info

Publication number
WO2018129486A3
WO2018129486A3 PCT/US2018/012849 US2018012849W WO2018129486A3 WO 2018129486 A3 WO2018129486 A3 WO 2018129486A3 US 2018012849 W US2018012849 W US 2018012849W WO 2018129486 A3 WO2018129486 A3 WO 2018129486A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
composition
gene expression
reporter gene
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2018/012849
Other languages
English (en)
Other versions
WO2018129486A2 (fr
Inventor
Tim D. Ahfeldt
Lee L. Rubin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of WO2018129486A2 publication Critical patent/WO2018129486A2/fr
Publication of WO2018129486A3 publication Critical patent/WO2018129486A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés, des vecteurs, des cellules transgéniques et des compositions pour exprimer un marqueur avec un gène d'intérêt. En particulier, l'invention concerne des procédés, des vecteurs, des cellules transgéniques et des compositions pour une expression élevée d'une protéine fluorescente telle que TDTomato avec un gène d'intérêt tel que la tyrosine hydroxylase afin d'évaluer l'expression du gène d'intérêt in vivo et in vitro.
PCT/US2018/012849 2017-01-06 2018-01-08 Composition et procédés pour une expression améliorée de gène rapporteur knock-in Ceased WO2018129486A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762443543P 2017-01-06 2017-01-06
US62/443,543 2017-01-06

Publications (2)

Publication Number Publication Date
WO2018129486A2 WO2018129486A2 (fr) 2018-07-12
WO2018129486A3 true WO2018129486A3 (fr) 2018-11-15

Family

ID=62790908

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/012849 Ceased WO2018129486A2 (fr) 2017-01-06 2018-01-08 Composition et procédés pour une expression améliorée de gène rapporteur knock-in

Country Status (1)

Country Link
WO (1) WO2018129486A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017180915A2 (fr) 2016-04-13 2017-10-19 Duke University Répresseurs à base de crispr/cas9 pour inactiver des cibles géniques in vivo et procédés d'utilisation
WO2018129486A2 (fr) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition et procédés pour une expression améliorée de gène rapporteur knock-in
EP4010485A4 (fr) * 2019-08-08 2023-08-23 Duke University Plate-forme de criblage à haut débit pour l'ingénierie de vecteurs de thérapie génique de nouvelle génération

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136597A (en) * 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
WO2015089419A2 (fr) * 2013-12-12 2015-06-18 The Broad Institute Inc. Délivrance, utilisation et applications thérapeutiques des systèmes crispr-cas et compositions permettant de cibler des troubles et maladies au moyen de constituants de délivrance sous forme de particules
US20160186213A1 (en) * 2013-06-17 2016-06-30 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
WO2018129486A2 (fr) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition et procédés pour une expression améliorée de gène rapporteur knock-in

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136597A (en) * 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
US20160186213A1 (en) * 2013-06-17 2016-06-30 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
WO2015089419A2 (fr) * 2013-12-12 2015-06-18 The Broad Institute Inc. Délivrance, utilisation et applications thérapeutiques des systèmes crispr-cas et compositions permettant de cibler des troubles et maladies au moyen de constituants de délivrance sous forme de particules
WO2018129486A2 (fr) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition et procédés pour une expression améliorée de gène rapporteur knock-in

Also Published As

Publication number Publication date
WO2018129486A2 (fr) 2018-07-12

Similar Documents

Publication Publication Date Title
AU2019264610A1 (en) Genetically modified animals and methods for making the same
MX2024008062A (es) Proteinas de enlace a mesotelina.
EP4442807A3 (fr) Milieu de culture cellulaire enrichi en taurine et procédés d'utilisation
EP4389889A3 (fr) Compositions et procédés d'édition d'arn
AU2018236742B2 (en) Compositions of adenosine deaminase-2 (ada2), variants thereof and methods of using same
MX2018010529A (es) Composiciones y metodos para la produccion y administracion de acido ribonucleico.
CY1120845T1 (el) Στοχευομενη τροποποιηση γονιδιωματος αρουραιου
CL2016003096A1 (es) Métodos para cosechar cultivos de células de mamífero
MX2019012311A (es) Metodos y composiciones para modificaciones geneticas dirigidas y metodos de uso.
MX2018001617A (es) Composiciones de crispr-cas9 diseñados y metodos de uso.
EP4296278A3 (fr) Animaux non humains ayant un groupe humanisé de gène de différenciation 47
EP4600650A3 (fr) Procédés et compositions améliorés pour biomarqueurs synthétiques
EP3980131A4 (fr) Compositions et méthodes associées aux engageurs de lympocytes t activés par une tumeur
CA3011049A1 (fr) Compositions pour lier des domaines de liaison a l'adn et des domaines de clivage
MX2016016903A (es) Animales no humanos que tienen un gen de muerte celular programada 1 humanizado.
BR112017007770A2 (pt) cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.
TR201806812T4 (tr) Rna-yönlendirmeli hedef dna modifikasyonu için ve rna-yönlendirmeli transkripsiyon modifikasyonu için yöntemler ve bileşimler.
EP4585692A3 (fr) Procedes d'insertion ciblee d'adn dans des genes
PH12021551100A1 (en) Dried biological compositions and methods thereof
SG10201803766UA (en) Lincrna-deficient non-human animals
WO2018129486A3 (fr) Composition et procédés pour une expression améliorée de gène rapporteur knock-in
WO2017044476A8 (fr) Méthodes et compositions pour la diversification génétique à base de recombinase
MX2018009513A (es) Animales no humanos que tienen un gen angptl8 modificado genéticamente.
WO2015181831A3 (fr) Procédé pour cibler un glioblastome avec des cellules souches mésenchymateuses de la gelée de wharton (cms-gw) dérivées de cordon ombilical humain
WO2020205626A8 (fr) Modulateurs d'interactions de protéine de surface cellulaire et procédés et compositions associés à ceux-ci

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18736701

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18736701

Country of ref document: EP

Kind code of ref document: A2