WO2018181380A1 - Edible composition for intestinal bacteria - Google Patents

Abstract

To provide an edible composition for intestinal bacteria which ameliorates intestinal flora (intestinal bacterial flora) and promotes the diversity thereof so as to contribute to human health. A composition comprising: a natural dietary fiber component, said natural dietary fiber component being eatable by intestinal flora and having preferable taste and flavor so that humans can take the same continuously; and hot water-extracts (dead cells) of Enterococcus faecalis AD101(FERM P-20739), Lactobacillus reuteri AD302 (FERM P-20741) and Lactobacillus acidophilus AD206. The composition preferably comprises, as a main component, a powdery or granular product obtained by homogeneously mixing an extract of Cichorium intybus with other dietary fibers such as fruits.

Description

Edible composition for enterobacteria

The present invention relates to an edible composition for intestinal bacteria constituting intestinal flora (intestinal flora). The present invention also relates to a health food containing such an edible composition for enteric bacteria and a method for producing the same.

While the intake of fat and sugar has increased due to the penetration of Western diets, the consumption of vegetables has drastically decreased, and the intake of dietary fiber consumed by intestinal flora has fallen below half that of 50 years ago.
Antibiotics frequently used in hospitals and dental clinics remain in the human body, damage the intestinal flora, and are used for edible cattle, pigs, broilers, etc., food additives, pesticides There are also situations where the chlorine component of tap water damages the intestinal flora.
Depending on the type of food additive, some food additives are harmless to humans, but some are harmful to intestinal bacteria.

As a result, the amount of human feces is reduced, the intestinal flora is reduced by the amount of reduced fecal amounts, and the intestinal environment is deteriorated due to the damage described above.
Changes in dietary life simultaneously increase so-called lifestyle-related diseases such as arteriosclerosis, heart disease, diabetes, etc., and medical costs are increasing with aging.

On the other hand, research on intestinal flora is progressing and elucidating (Non-Patent Document 1). For example, as a result of research, the following effects, influences, and possibilities have been found.

1. Obesity is not necessarily caused by over calories, but is caused by fat cell hypertrophy. Enterobacteria produce short-chain fatty acids that eliminate obesity, but as mentioned above, enterobacteria themselves have deteriorated due to westernization of their diet, etc., so the ability to produce short-chain fatty acids is reduced, Obese patients continue to increase.
In addition, the short-chain fatty acids produced by the intestinal flora stimulate the intestinal cells and promote the secretion of an incretin hormone that works to secrete insulin from the pancreas, but the deterioration of the intestinal flora is the production of short-chain fatty acids. Since the amount is decreased, the amount of insulin secreted is reduced, which causes diabetes.

2. A decrease in the amount of short-chain fatty acids produced due to deterioration of the intestinal bacteria leads to deterioration of the barrier layer of the intestinal tract, causing a gap (leaking intestine) in the intestinal tract. Harmful bacteria and toxins in the intestine are released to the outside of the intestine through the gaps in the intestine. Macrophages such as leukocytes react with toxins leaking outside the intestinal tract by secreting inflammatory cytokines, etc., and the whole body is in a mild inflammatory state.
Insulin is reduced in the vicinity of inflamed cell tissues, causing diabetes.

3. Obesity increases the number of Ariake, one of the enteric bacteria. Ariake senescent cells (irreversible cell growth arrest induced by constant activation of the cell cycle checkpoint mechanism when normal cells undergo irreparably large DNA damage) DCA (deoxycholic acid), which is a substance that induces cancer such as liver cancer, breast cancer, and esophageal cancer, is increased in the body.

4). “Equol” is a substance produced by intestinal bacteria eating soy components (isoflavones), and also a substance that keeps skin healthy, and works similar to female hormones, but the intestinal flora that produces this is The number of humans (permanent in about half of Japanese people) is decreasing year by year. Since equol binds to the estrogen receptor, it suppresses the stimulation of the mammary gland by excessively secreted estrogen, reducing the probability of breast cancer, but when equol decreases, the number of estrogen bound to the estrogen receptor Is believed to increase the chances of over-stimulating the mammary gland and causing breast cancer. "Equol" suppresses the activation of the male hormone testosterone to become dihydrotestosterone, prevents excessive dihydrotestosterone, and binds to dihydrotestosterone and inhibits binding to the androgen receptor. It has the action of suppressing androgen that causes prostate cancer.
With the reduction of “equol”, prostate cancer is thought to increase as well.

5). Degradation of the intestinal flora reduces the amount of short-chain fatty acids that are produced, causing the immune cells (leukocyte T cells) that cause allergies to run away.
That is, immature leukocyte T cells are changed to regulatory leukocyte T cells (T-leg cells) by butyric acid (short chain fatty acid). T-reg cells suppress the activity of mature leukocyte T cells and suppress excessive reaction (runaway) of leukocyte T cells, but as the intestinal flora deteriorates, the production of short chain fatty acids decreases, Since leukocyte T cells that change to T-leg cells also decrease, the activity of leukocyte T cells becomes uncontrollable and runaway, increasing allergies and autoimmune diseases.

6. When the intestine leaks, toxins and harmful bacteria leak out of the intestinal tract through the gaps in the intestinal wall, so the macrophages such as leukocytes react with the toxins outside the intestinal tract by secreting inflammatory cytokines, and the whole body is light. It becomes inflamed. Due to this prolonged inflammation, macrophages such as leukocytes become hypersensitive and leukocytes that begin to attack by phagocytosing so-called foreign substances that have not been attacked until now, resulting in dead leukocytes that are insufficiently digested and digested by foreign substances The macrophages accumulate and cause arteriosclerosis.

7). Intestinal flora produces neurotransmitters that nerve cells use in transmitting stimulus signals. Neurotransmitters include “serotonin”, “dopamine”, and the like, which are responsible for mind and body stability, will and improvement.
The neurotransmitter produced by the intestinal flora stimulates the brain directly via vagus cells, and as a result, the amount of neurotransmitter produced decreases with the deterioration of the intestinal flora, causing depression and other mental disorders. It becomes.
Moreover, it is thought that the possibility that the autism develops and becomes serious is increased by including the toxin 4EPS (4-ethylphenyl sulfate) produced by intestinal bacteria in the blood by the leaking intestine.
Intestinal flora is thought to dominate the brain by its product.

8. The number of human genes is 23,000, which is not much different from Daphnia, mice, etc., whereas the intestinal flora that normally resides in the intestine has 12 million genes, 99% of the necessary metabolites are produced by the intestinal flora.

9. Intestinal flora synthesizes and produces vitamin K, a blood clotting component that humans cannot make. Deterioration of the intestinal flora reduces the amount of blood clotting components produced, which can make hemostasis difficult.

10. Intestinal flora produces folic acid, vitamin B12, which forms red blood cells, and is responsible for human life activities. Deterioration of the intestinal flora reduces the amount of folic acid and vitamin B12 produced and lowers the ability to form blood cells, which may cause cerebral anemia and mental illness.
And so on.

JP-A-61-5022 discloses the relationship between intestinal flora and biological activity, and enterococcus bacteria useful for humans such as Enterococcus faecalis dead cells and Enterococcus lactobacilli, Bifidobacterium. It is described that there is a proliferation effect. JP-A-61-5022 and related scientific publications at that time make handling of intestinal bacteria, which can be provided only with pharmaceuticals, more complicated than live bacteria and more familiar.

Japanese Patent Application Laid-Open No. 3-151854 discloses that a mixture of oligosaccharides and soluble dietary fiber can reduce enterobacterium harmful fungi Clostridium genus and Escherichia genus.

JP 2012-111771 A discloses a dietary fiber preparation as a food or pharmaceutical product containing partially hydrolyzed guar gum (PHGG), wherein the second soluble fiber contains fructooligosaccharide (FOS) and has improved prebiotics ability. Is disclosed.

JP-T-2016-505533 discloses a grain as an ingredient that may be used in Chinese medicine such as whole grains, beans, nuts, peanuts, corn (eg, yellow corn, white corn, red corn, purple corn, etc.). Beneficial intestinal microorganisms formed by a food material selected from the first to third compositions among the first composition, the second composition made of bitter gourd, and the third composition made of these fermented products Intestinal flora improving compositions that enhance flora and inhibit opportunistic pathogens have been shown.

JP 2011-501940 discloses a bacterium of bifidobacteria (bifidobacterium) by containing a ternary mixture of a viscous water-soluble fiber, a non-viscous water-soluble fiber, and a water-insoluble fiber. Food compositions that increase the number and reduce the number of pathogenic bacteria (such as coliforms and Salmonella) have been disclosed.

Japanese Patent Application Laid-Open No. 2005-154388 discloses an intestinal function improving agent containing cyclofructan, which is a cyclic inulooligosaccharide composed of fructose molecules cyclically linked by β-2,1 bonds, as an active ingredient. .

Japanese Patent Application Laid-Open No. 2007-320946 describes that enterococcus faecalis, Lactobacillus reuteri and whey, root kelp, and other natural food powders are combined to increase the growth of useful bacteria and reduce harmful bacteria. ing.

Japanese Patent Publication No. 2014-513965 discloses that a dietary fiber composition such as fructooligosaccharide, indigestible dextrin, and polydextrose can be used to lengthen the fermentation period of dietary fiber in the large intestine and realize a comfortable bowel movement. Is disclosed.

Non-patent document 2 shows that when lactose and inulin are increased by 20 g / day and then ingested for 19 days, intestinal bacterial environment and bowel movement are improved. Non-patent document 3 describes that the pH of feces was lowered by ingestion of inulin-containing cereal meal.

JP-A-2016-172761 describes improvement of bowel movement with a composition containing inulin, indigestible dextrin, lactulose and the like, as in Non-Patent Document 1.

JP-A-61-5022 Japanese Patent Laid-Open No. 3-151854 JP 2012-111171 A Special table 2016-505533 Special table 2011-501940 gazette JP 2005-154388 A JP 2007-320946 A Special table 2014-513965 gazette JP 2016-172761 A

“The Truth of Intestinal Flora 10,” NHK Special Coverage, published by Shufu no Tomosha, August 28, 2015 Effects of inulin and lactose on fecal microflora, microbial activity, and bowel habit in elderly constipated persons., Kleessen B1, et.al., Am J Clin Nutr. 1997 May; 65 (5): 1397-402. Effect of consumption of a ready-to-eat breakfast cereal containing inulin on the intestinal milieu and blood lipids in healthy male volunteers., Brighenti F1, Casiraghi MC, Canzi E, Ferrari A;, Eur Jr.53 9): 726-33.

As described above, scientific elucidation of intestinal flora has progressed, and proposals for improving intestinal flora and health foods composed of various dietary fibers have been proposed as shown in the technical literature and technical reports. However, there are still no proposals to increase the diversity of intestinal flora.

“Diversity” of intestinal flora indicates, for example, the number of types of intestinal bacteria. “Increase” means, for example, the growth and activation of intestinal bacteria, which is a function of the intestinal flora that has been lost in the past due to the increase in the amount of defecation or deterioration of the intestinal flora. Or may include regaining functionality that may be needed.

Although the function of intestinal bacteria has many unexplained functions, the diversity shown in the present invention is the type of intestinal bacteria that produce useful substances such as short-chain fatty acids and equol, and their intestines. It may be preferable to show an increase in the intestinal flora formed by the internal bacteria. In addition, there are many enterobacteria that have a certain role for humans, even in the case of enterobacteria that are generally classified as genus Clostridium and Escherichia. In some cases, the increase in the presence of bacteria can be shown as the diversity of enteric bacteria.

In addition, the diversity of intestinal flora may indicate a variety of beneficial intestinal bacteria, or a balanced state of harmful bacteria, useful bacteria, and opportunistic bacteria.

The present invention has been made in view of the above, and as a result of earnest research on the relationship between enterobacteria, which are now elucidated, and humans,
It is not the so-called “natural movement theory” that humans make use of intestinal flora, but intestinal flora makes use of humans, that is, the intestines that human health is protected only after the health of intestinal flora is created. The inventor has arrived at the present invention by finding that the inner flora should be based on the so-called “ground motion theory”.

The present invention relates to an edible composition comprising a dietary fiber and / or an enterobacterial hot water extract that can be eaten stably or without resistance by human beings, which are mainly eatable by intestinal bacteria. Realize the provision of (food).

Examples of the foods eaten by the intestinal bacteria and the intestinal flora include dietary fiber.
The intestinal flora shown in the present invention is a collection of intestinal bacteria, but when explaining its functionality, it shows the functionality of the intestinal bacteria alone.

Examples of the dietary fiber include dietary fiber alone or natural or synthetic foods containing dietary fiber. Ingredients are preferably natural or naturally derived in that enteric bacteria and intestinal flora increase diversity and produce many effective products.

Dietary fiber includes both water-soluble dietary fiber and water-insoluble dietary fiber.
In addition, the dietary fiber shown in the present invention includes either or both of synthetic dietary fiber and naturally-occurring dietary fiber, but the synthesized dietary fiber lacks the functions and components of the naturally-derived dietary fiber. In some cases, digestive absorption in the body is not sufficient, and natural dietary fiber may be preferred because it may not be a preferred food (assistant) for intestinal bacteria and intestinal flora.

More specifically, the dietary fiber in the present invention is, for example, a composition in which enterobacteria are fermented and absorbed in the intestinal tract, that is, an enterobacterial food (utilized product), wherein the intestinal bacteria are short-chain fatty acids. By using an edible composition as a component for producing, the diversity of enteric bacteria can be increased.

Examples of foods that produce short-chain fatty acids include naturally derived inulin, but those purified and extracted from chicory, chrysanthemum, burdock, leek, etc., and raw material components of chicory root, chrysanthemum, burdock, and leek Therefore, a natural extract containing a predetermined amount (for example, 70% or more) of inulin is more preferable in that it has sufficient natural dietary fiber capacity and can be obtained at a lower cost.

In addition, when it is difficult for humans to eat stably only with foods that produce short-chain fatty acids, it is preferable to further contain fruit juice, honey, and fruit-derived dietary fibers that adjust the taste and texture.
In this case, the dietary fiber related to the fruit can also increase the diversity of the intestinal flora in the same manner as the natural dietary fiber, and as a whole can be an assimilated composition suitable for the intestinal flora. .

“Stable” includes not only eating at regular intervals every day, but also to the extent that it is possible to eat even if it is a single shot, and to eat again.
In addition, since it is preferable not to use as much as possible degradation factors of enteric bacteria such as food additives, if there is a bitter taste or a unique texture or taste of other ingredients themselves, more uniform mixing of ingredients and granulation This makes it possible for humans to eat stably.

As described above, short-chain fatty acids produced by intestinal bacteria prevent fat cell hypertrophy and eliminate obesity, and thus prevent liver cancer, breast cancer, esophageal cancer, etc. caused by obesity, and regulatory T cells. To increase the production ability of the leukocytes, suppress the runaway of white blood cell T cells that cause allergies, improve the barrier function of the intestines, and prevent leaking intestines.

As described above, the “leaking intestine” is a decrease in the ability of the intestinal bacterium to produce short-chain fatty acids due to the deterioration of the intestinal bacterium, thereby reducing the intestinal barrier function and creating a gap in the intestinal tract. This means that harmful bacteria and toxins leak into the blood. Because leukocytes and macrophages attack the leaked toxins and bacteria in the blood, the whole body becomes mildly inflamed, inhibiting insulin activity and stimulating intestinal cells. As a result of the attack by phagocytosis, dead bodies such as leukocytes accumulate in the blood vessels and cause arteriosclerosis.

Obesity also increases Ariake bacteria, which is likely to induce cancer, one of the enteric bacteria.
Therefore, the present invention has realized providing an edible composition containing foods of enteric bacteria that promotes the production of short-chain fatty acids by making enteric bacteria healthy and increasing diversity. .

Short chain fatty acids are composed of acetic acid, propionic acid, butyric acid, their esters, and their salts, and are mainly produced by intestinal flora. The present invention is intended to compensate for the production of short chain fatty acids in intestinal flora. In some cases, short-chain fatty acids may be added separately.

Examples of enterobacterial foods that produce short-chain fatty acids (assimilation compositions) include inulin as described above, and examples of natural inulin-containing materials include chicory, chrysanthemum, burdock, and leek. It is.
The extract of chicory root is particularly preferable in that it contains a large amount of inulin derived from nature, and is superior in absorbability and functionality compared to synthesized inulin. Synthetic dietary fibers generally have stronger adsorption capacity than natural ones, and use natural inulin because there is a risk that minerals necessary for humans may be discharged outside the body. It may be preferable.

Furthermore, the present invention provides an increase in the diversity of intestinal flora by providing a substance that can be ingested stably by humans, which is a substance that is mainly consumed by enterobacteria to activate intestinal flora. Is realized.

In order to increase the diversity of intestinal flora, the present invention comprises 25 to 70% (preferably 40% to 50%) of chicory root powder and chrysanthemum powder with respect to the total weight, fruit components Examples (Examples 1 and 2) include 10-50% (preferably 25% -40%), buckwheat, guar gum which is a thickener and dietary fiber, and enterobacterial bacterial powder. Is done.
As the guar gum used as a thickener, it is preferable to use other natural gums that function as dietary fibers.
The chicory root extract is rich in inulin, which is a water-soluble dietary fiber, and is suitable for increasing fecal excretion efficiency.

Chicory is a herb-based vegetable and, depending on the degree of extraction, has characteristics of bitter ingredients such as lactupirin, flavor, and fragrance. In order to achieve this, it is necessary to devise measures such as mitigating, but the present invention, after powdering these, add fruit powder, and by mixing uniformly and granulating, the natural origin such as bitterness It is possible to provide a composition that can relax a unique taste and can be stably eaten by humans. As a means for uniformly mixing, a fluidized bed granulation method is preferable, and a powdered food that has good uniformity of granulation components and is bitter and can be eaten mellowly and stably can be formed.

Inulin includes synthetic inulin obtained by enzymatic synthesis using sugar as a raw material, as well as naturally-derived inulin extracted from chicory, chrysanthemum, etc., but synthetic inulin is inexpensive and highly pure. From the nature, it is thought that there is a problem that it is difficult to be assimilated by intestinal bacteria because it is considered that the components derived from nature are insufficient and the digestive absorption in the gastrointestinal tract is poor. In contrast, naturally-occurring inulin may be affected by the weather and yield, and the quality may not be stable, but it can maintain the original components of inulin and is well absorbed by the body. It is thought that it is excellent in utilization.

However, among the natural inulins, the inulin derived from chrysanthemums is not suitable for long-term storage because it contains components that degrade inulin in the chrysanthemum salmon itself. A chicory root extract is preferred as the component to be increased. In order to form supplemental food forms using natural materials, it is necessary to control the taste and smell of natural materials. This control can be suppressed by some additives, but some food additives may damage intestinal bacteria and may have the opposite effect. It is preferable to suppress the use of.

Therefore, by using granulation means with uniform ingredients and a uniform particle size, so-called naturally-derived taste, odor, etc. can be suppressed, and foods that can be eaten stably even by humans can be manufactured. It becomes.

In addition to natural dietary fiber that improves the ability to produce short chain fatty acids, β-glucan, fructan, albinoxylan, konjac glucomannan, hemicellulose (soy polysaccharides, rice bran, brown rice, corn hulls), polydextrose, lignin, High methoxy pectin, oligosaccharide: raffinose, stachyose (galacto-oligosaccharide) fructooligosaccharide, maltotriose, maltotetraose, maltopentaose, fucoidan (seaweed such as mekabu, kelp, mosque), water-soluble soybean polysaccharide, pullulan, etc. The dietary fiber may increase the diversification of intestinal flora, so it may be included in an edible composition that humans eat stably.

The present invention is a food effective for enterobacteria, preferably a naturally occurring dietary fiber that can be stably eaten by humans, Enterococcus faecalis AD101 (FERM P-20739), Lactobacillus reuteri AD302 ( FERM P-20741) and an edible composition containing a hot water extract (dead cells) of Lactobacillus acidophilus AD206 increases the diversity of intestinal flora, thereby realizing human health.

It is a component that eats the intestinal flora, makes the intestinal flora healthy (increases its activity), and reversibly increases diversity, as long as the component can be stably ingested by humans. Are preferably enteric bacterial foods that produce short-chain fatty acids that are beneficial to humans.

In this embodiment, natural dietary fiber that enteric bacteria prefer to eat dietary fiber components such as chicory, chicory root extract, chrysanthemum, etc., fruit with a taste that can be eaten stably by humans, etc. It is preferable that the edible composition is a combination of both dietary fibers.

The chicory, chicory root extract or chrysanthemum pod in the present invention contains a lot of inulin, is eaten by intestinal bacteria, and is decomposed into short chain fatty acids. Since chrysanthemums contain ingredients that degrade inulin, securing an effective amount of inulin requires purified extraction and may lack naturalness. Suitable as food.

Inulin can absorb sugar in the body because it takes in sugar in the process of becoming a swollen gel when it contacts water.
Therefore, by increasing the amount of inulin to 4 g or more as shown in the examples, the range of taste as a food can be widened, and it is easy for humans to take and can be taken daily.

In addition, although an inulin-containing natural food ingredient has been shown as a main component as an embodiment of the present invention, barley, buckwheat, guar gum, other thickening polysaccharides, glucomannan, pectin, hemicellulose, lignin, alginic acid, polydextrose, Natural foods and extracts thereof containing water-soluble dietary fiber such as indigestible dextrin, resistant starch, fucoidan, water-soluble soybean polysaccharide, pullulan and their degradation products may be used. A granulated product may be formed by combining flavor-adjusting dietary fiber materials for realizing a taste, flavor, and texture that can be eaten stably.

For flavor adjustment, it is preferable not only to adjust the flavor, but also to increase the diversity of intestinal flora, and the natural food alone containing the above-mentioned dietary fiber that increases the diversity of intestinal flora Even if it is an edible composition obtained by processing granulated powder, it may be good or may be suitable if it can be eaten stably by humans.

The intestinal bacterial hot water extract in the present invention represents a hot water extract (dead cell) of Enterococcus faecalis AD101 (FERM P-20739), Lactobacillus reuteri AD302 (FERM P-20741), and Lactobacillus acidophilus AD206. . As described in, for example, JP-A-61-5022 and JP-A-2007-320946, they exhibit the ability to grow useful bacteria, and in combination with natural dietary fiber, It is possible to further increase the diversity of internal bacteria.
Examples of the hot water extraction method include the method shown in JP-A-61-5022.

As a form of the food, a powder using a fluidized bed granulation method with a uniform particle size and capable of mixing the uniform components may be further granulated, made into a capsule shape, a sugar coating shape, or an aqueous solution shape.
Further, it may be processed into sprinkles, soft drinks, biscuits, rice crackers and the like.

Enterococcus faecalis AD101 (FERM P-20739) and Lactobacillus reuteri AD302 (FERM P-20741) are deposited at the Patent Biological Depositary Center based on their respective deposit numbers.

Subsequently, the present invention will be described with reference to the examples. In addition, this invention is not limited by these Examples.

Examples 1 and 2
Examples 1 and 2 were carried out with the compositions shown in Table 1.

Example 1 shown in Table 1 shows that chicory root extract (90% inulin) is 20% based on the total weight%, chrysanthemum pod 5.2%, oat 6%, guar gum 3%, apple, mango, A powder consisting of 32% fruit components containing dietary fiber such as banana, etc., is a food that is eaten by intestinal bacteria by removing the bitterness inherent in chicory by uniform powdering by fluidized bed granulation technique, An edible composition that can be stably eaten by humans is produced.

Using guar gum (3% of the total weight) that is also a dietary fiber as a binder using a fluidized bed flow generator, spraying hot air from the lower side of the raw material powder in the granulation chamber while spraying it. In some cases, the particles are granulated while fluidizing them on a rotating plate to form powders or granules having uniform particle diameters and components.

This granulation method uniformly mixes chicory root extract, fruit components, etc. to produce powders or granules having a more uniform particle size. Therefore, the taste unique to chicory root is alleviated and eaten by humans. Form edible edible compositions.

Ingestion of edible composition powder 10 g containing the ingredients shown in the examples of Table 1 at a time, once to 5 times a day, preferably 3 times a day By continuing this, the amount of defecation increases, the diversity of intestinal flora is recovered, and human health is achieved along with the health of intestinal bacteria.

In this example, chicory root extract was used as inulin, but it may be extracted from chrysanthemum extract, other burdock, and leek, but is preferable for assimilation of intestinal bacteria, To increase diversity, increase the amount of defecation as much as possible without using synthetic food additives as much as possible (10g-30g a day), and the stability of the product for continuous use Because it is preferably maintainable, chicory root extract is preferred for intestinal bacteria and intestinal flora with high inulin content.

Since chicory has a bitter component, it can be finely pulverized and then mixed uniformly with dietary fiber components with sweetness such as other fruits using a fluidized bed granulation method. It becomes a health food that can contribute to the assimilation of intestinal bacteria while suppressing additives, and that can be continuously received by humans.

Example 2 in Table 1 has approximately twice the amount of chicory root extract compared to Example 1.
Using the fluidized bed granulation technique used for the manufacture of pharmaceuticals, these powders form a uniform state and are suitable for humans to continuously eat foods of intestinal bacteria while suppressing bitterness. Can be obtained.

10 g of powdered or granulated food prepared by pulverizing these ingredients (Example 1) is taken as a single intake, eaten as it is, or dissolved in milk, water, etc. Ingest about 5 times. Since humans eat a considerable amount of dietary fiber such as inulin, the intestinal microflora eats these naturally-derived inulin-containing foods that reach the intestines, generating short-chain fatty acids and increasing the amount of defecation The effect of short-chain fatty acids is demonstrated by improving the activity of the intestinal flora, but the sensory test on the ingredients shown in Example 1 having a uniform particle size and powder as a component, and a granular powder food According to the results of (10 test subjects), although there are some sweetnesses, there are many respondents with 8 people that the bitterness and flavor peculiar to chicory are suppressed and that they have a mellow texture. More than half of the products were evaluated as foods without resistance. As for the improvement and frequency of bowel movements, 7 to 8 people answered that the number of bowel movements increased.

Examples 3 and 4
Examples 3 and 4 were carried out with the following composition.

[Composition of Example 3]
Barley (endosperm) 20%
Guar gum 3%
Fruit juice ingredient powder (dietary fiber) 60%
Enterococcus faecalis AD101 (deposit number P-20739) 2.82%
Lactobacillus reuteri AD302 (deposit number P-20741) 0.2%
Lactobacillus acidophilus AD206 0.1%
Others (honey powder, etc.) 13.88%
Total 100%

[Composition of Example 4]
Soybean hypocotyl extract (extract) powder 10%
Guar gum 3%
Fruit juice component powder (dietary fiber) 70%
Enterococcus faecalis AD101 (deposit number P-20739) 2.82%
Lactobacillus reuteri AD302 (deposit number P-20741) 0.2%
Lactobacillus acidophilus AD206 0.1%
Others (honey powder, etc.) 13.88%
Total 100%

Example 3 uses barley as dietary fiber, and fruit juice fiber and other components are fruit fibers such as banana, apple and mango, and honey and the like are shown as in Examples 1 and 2. .

Enterococcus faecalis AD101 (deposit number P-20739), Lactobacillus reuteri AD302 (deposit number P-20741), and hot water extract (dead cells) of Lactobacillus acidophilus AD206, as described above, have the ability to grow useful resident bacteria. Therefore, it has a function to further increase the diversity of intestinal bacteria resident in the intestine.

As a single intake, it is preferable to take 10 g to 30 g / pack three times a day, but it is preferable to take in consideration of the constitution.

Barley (endosperm portion) contains a large amount of water-soluble dietary fiber β-glucan and, like inulin, has the function of increasing the diversity of intestinal bacteria and increases the amount of feces.

The barley (endosperm) powder is preferably obtained by separating the barley endosperm portion. The barley flour is extracted by hot water extraction and solid separation, or air separation or sieving.
In addition to barley, buckwheat (oat) may be used as in Examples 1 and 2.

As a manufacturing method, barley powder, fruit juice component powder, etc. are made into powder or granules having a uniform component and particle size by using a fluidized bed granulation technique using guar gum.

The above examples showed powders or granules formed, but in addition, tablets, rods, plates, blocks, solids, rounds, liquids, bowls, pastes, creams , Capsules like hard capsules and soft capsules, caplets, tablets, gels, jellies, and other food forms may be produced, but at least they can be changed to these forms after being powdered preferable.

As in this example, by containing a lot of barley (endosperm) extract powder having a high natural origin, it is possible to relieve the original miscellaneous taste of the barley and to allow humans to eat stably and continuously.

Example 4 contains about 10% to 50% soy hypocotyl extract powder containing a large amount of isoflavone, which is a raw material for producing equol in the intestine. Soybean hypocotyl extract has bitterness, sashimi, etc., so as to alleviate this and improve the taste and texture, use food additives as much as possible, and further adjust dietary fiber components such as fruit juice components The added powder is mixed and a powder or granule having a uniform particle size and components is formed by a fluidized bed granulation technique.

Soybean hypocotyl extract also relieves bitterness compared to the hypocotyl itself, but in order for humans to eat stably, it is preferable to have a mellow texture that further reduces bitterness.

As in this example, the powder blended with fruit dietary fiber has a more uniform particle size and a uniform component, so that the taste becomes mellow and can be continuously eaten.

Example 5
In this example, an intake test was performed according to the following procedure.
For 20 subjects who showed constipation symptoms and were treated for atopy and other diseases, powdered foods having the ingredients shown in Example 2 of Table 1 were ingested daily (from 1st to several days). Then, after ingestion, a questionnaire survey was conducted on each subject for subsequent bowel movements.

According to the results of the questionnaire, all 20 people had bowel movements after the start of eating, and the amount of bowel movements and the number of bowel movements were large. All subjects ate continuously and had no trouble. In addition, after the start of feeding, the disease did not worsen, and it went in the good direction as well as good bowel movements. Table 2 below summarizes the specific status of defecation for 20 people with improved bowel movements.

From the results shown in Table 2, continuously eating a powdered food containing at least 4 g of inulin (derived from chicory root) and enterobacterial bacterial cells at a time as a normal processed food at least once a day. Turned out to be possible.

Finally, preferable modes of the present invention are summarized as follows.
[Appendix 1]
An edible composition for enteric bacteria comprising a dietary fiber that is eatable by enteric bacteria and that can be stably consumed by humans.
[Appendix 2]
The edible composition for enteric bacteria according to appendix 1, further comprising a hot water extract of Enterococcus faecalis AD101 (FERM P-20739), Lactobacillus reuteri AD302 (FERM P-20741) or Lactobacillus acidophilus AD206 or a combination thereof .
[Appendix 3]
The edible composition for enteric bacteria according to appendix 1, wherein a short-chain fatty acid can be produced from the dietary fiber by the enteric bacteria.
[Appendix 4]
The edible composition for enteric bacteria according to appendix 1, wherein the dietary fiber is one or more members selected from the group consisting of inulin, β-glucan, lecithin, fructan, and guar gum.
[Appendix 5]
The dietary fiber is selected from the group consisting of powder of natural cereal material consisting of burdock, leek, chrysanthemum, chicory, barley, buckwheat or guar gum or powder thereof and banana, apple, mango or peach fruit powder. The edible composition for enteric bacteria according to appendix 1, wherein the edible composition is one or more members.
[Appendix 6]
The dietary fiber is one or more members selected from the group consisting of fucoidan, fructooligosaccharide, arabinoxylan, hemicellulose, levan, soybean oligosaccharide, and konjac glucomannan, and is extracted from natural foods. The edible composition for enteric bacteria according to appendix 1.
[Appendix 7]
The dietary fiber includes naturally occurring galactooligosaccharides, fuco-oligosaccharides, manno-oligosaccharides, xylo-oligosaccharides, sialyl-oligosaccharides and other oligosaccharide compositions, fructan, galactan, fucoidan, arabinan, xylan, xanthan, galacturonan, N-glycan Includes one or more members selected from the group consisting of, О-glycan, hyaluronic acid, chondroitin, xyloglucan, arabinogalactan, alginate, carrageenan, galactomannan, arabinoxylan, glycolipid glycan, glycoprotein glycan, and proteoglycan The edible composition for enteric bacteria according to appendix 1, wherein the extracted natural food or an extract obtained by extracting the natural food is included.
[Appendix 8]
The edible composition for enteric bacteria according to appendix 1, wherein the dietary fiber material is any of a powdery material, a granular material, a solid material, or a liquid material uniformly mixed by fluidized bed granulation.
[Appendix 9]
The edible composition for enteric bacteria according to appendix 1 or 2, further comprising acetic acid, propionic acid, butyric acid or an ester thereof or a salt thereof as an additive.
[Appendix 10]
In the above dietary fiber, chicory root extract is 25 to 50% by weight of the total weight, chrysanthemum rice cake is 2 to 5% by weight, barley or buckwheat is 5% by weight, fruit juice and powder is 15 to 30% by weight, intestine The edible composition for enteric bacteria according to appendix 1 or 2, wherein the bacterial hot water extract powder is 3 to 5% by weight, and the dietary fiber is uniformly mixed and granulated or powdered object.
[Appendix 11]
A health food comprising the edible composition for enteric bacteria according to appendix 10.
[Appendix 12]
The method for producing a health food according to supplementary note 11, comprising a step of uniformly mixing the dietary fiber and granulating or powdering the obtained mixture.

The present invention relates to dietary fiber suitable for intestinal flora to eat (utilize) and the heat of Enterococcus faecalis AD101 (deposit number P-20739), Lactobacillus reuteri AD302 (deposit number P-20741), and Lactobacillus acidophilus AD206. By providing an edible composition containing an aqueous extract (dead cells) in a form that is easy for humans to take, the diversity of intestinal flora in the human body can be increased, thus providing new health Provide food.

Claims (12)

An edible composition for enteric bacteria comprising a dietary fiber that is eaten by enteric bacteria and that can be stably consumed by humans. The edible composition for enteric bacteria according to claim 1, further comprising a hot water extract of Enterococcus faecalis AD101 (FERM P-20739), Lactobacillus reuteri AD302 (FERM P-20741) or Lactobacillus acidophilus AD206 or a combination thereof. object. The edible composition for enteric bacteria according to claim 1, wherein short-chain fatty acids can be produced from the dietary fiber by the enteric bacteria. The edible composition for enteric bacteria according to claim 1, wherein the dietary fiber is one or more members selected from the group consisting of inulin, β-glucan, lecithin, fructan and guar gum. The dietary fiber is selected from the group consisting of powder of natural cereal material consisting of burdock, leek, chrysanthemum, chicory, barley, buckwheat or guar gum or powder thereof and banana, apple, mango or peach fruit powder. The edible composition for enteric bacteria according to claim 1, wherein the edible composition is one or more members. The dietary fiber is one or more members selected from the group consisting of fucoidan, fructooligosaccharide, arabinoxylan, hemicellulose, levan, soybean oligosaccharide, and konjac glucomannan, and is extracted from natural foods. The edible composition for enteric bacteria according to claim 1. The dietary fiber includes naturally occurring galactooligosaccharides, fuco-oligosaccharides, manno-oligosaccharides, xylo-oligosaccharides, sialyl-oligosaccharides and other oligosaccharide compositions, fructan, galactan, fucoidan, arabinan, xylan, xanthan, galacturonan, N-glycan Includes one or more members selected from the group consisting of, О-glycan, hyaluronic acid, chondroitin, xyloglucan, arabinogalactan, alginate, carrageenan, galactomannan, arabinoxylan, glycolipid glycan, glycoprotein glycan, and proteoglycan The edible composition for enteric bacteria according to claim 1, wherein the natural food material or an extract obtained by extracting the natural food material is contained. The edible composition for enteric bacteria according to claim 1, wherein the dietary fiber is one of a powder, a granule, a solid, and a liquid that are uniformly mixed by fluidized bed granulation. . The edible composition for enteric bacteria according to claim 1 or 2, further comprising acetic acid, propionic acid, butyric acid or an ester thereof or a salt thereof as an additive. In the above dietary fiber, chicory root extract is 25 to 50% by weight of the total weight, chrysanthemum rice cake is 2 to 5% by weight, barley or buckwheat is 5% by weight, fruit juice and powder is 15 to 30% by weight, intestine The edible food for enteric bacteria according to claim 1 or 2, wherein the bacterial hot water extract powder is 3 to 5% by weight, and the dietary fiber is uniformly mixed and granulated or powdered. Composition. A health food comprising the edible composition for enteric bacteria according to claim 10. The method for producing a health food according to claim 11, comprising a step of uniformly mixing the dietary fiber and granulating or powdering the obtained mixture.