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WO2018174669A1 - Produit fermenté de colostrum et composition cosmétique l'utilisant - Google Patents

Produit fermenté de colostrum et composition cosmétique l'utilisant Download PDF

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Publication number
WO2018174669A1
WO2018174669A1 PCT/KR2018/003492 KR2018003492W WO2018174669A1 WO 2018174669 A1 WO2018174669 A1 WO 2018174669A1 KR 2018003492 W KR2018003492 W KR 2018003492W WO 2018174669 A1 WO2018174669 A1 WO 2018174669A1
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Prior art keywords
colostrum
fermentation
composition
lactobacillus
fermented
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English (en)
Korean (ko)
Inventor
곽태일
김준혁
김광일
서상아
서혜령
안용대
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Farm Skin Inc
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Farm Skin Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/986—Milk; Derivatives thereof, e.g. butter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • the present invention relates to a fermented product of colostrum and a cosmetic composition using the same, and more particularly to fermented products of colostrum obtained by fermenting colostrum with fermentation bacteria, specific lactic acid bacteria, or complex bacteria and anti-inflammatory and skin irritation using the same.
  • the present invention relates to a functional raw material composition obtained by a new composition having effects such as whitening, moisturizing, and wrinkle improvement, and is applicable to cosmetic compositions, food compositions, pharmaceutical compositions, and the like.
  • the skin consists of the epidermis and the dermis.
  • the epidermis is constantly dividing cells, forming a variety of layers.
  • the outermost cells die and become hard, which is one of the best defenses to protect the body from external stress, and bacteria can't penetrate the skin.
  • the skin has sebaceous glands and sweat glands, the sebaceous glands secrete sebum, and the glands secrete water to create sebum membranes and protect the stratum corneum. Therefore, human skin is always moist, glossy and smooth. It becomes possible.
  • Cosmetics are intended to protect the skin from the outside, remodeling, and cleanliness, but when you look closely at the composition of the cosmetics, the ingredients that are necessary for the formation of the product, contrary to the original meaning, may be irritating to the skin. have. Examples include emulsifiers, fragrances and preservatives. They are most often made by chemical reaction synthesis, which increases the chances of inflammatory reactions, allergies and erythema in sensitive skin.
  • Lactic acid bacteria refers to bacteria that break down carbohydrates into lactic acid by metabolism. If the lactic acid is increased, the acidity is lowered, and the acidity occurs when the lactic acid bacteria themselves die. Therefore, fermentation time is important, and it is also a method to be resistant to oxidative stress.
  • Lactic acid produced by lactic acid bacteria plays a very large role. Antioxidant, anticancer, anti-radioactivity detoxification role and also has anti-inflammatory effect. It also has the ability to increase the water binding of the stratum corneum, which can improve fine wrinkles and soften the skin.
  • Interleukin 1 is a type of cytokine that is produced by several cells, including activated monocytes, epithelial cells, or vascular endothelial cells.
  • Another representative inflammatory component is the cytokine Tumor Necrosis Factor- ⁇ (TNF- ⁇ ).
  • Interleukin-10 inhibits the expression of inflammation-related enzymes, such as inducible nitric oxide synthase (iNOS) and inducible cyclooxyganase (COX-2), which are present in macrophages (Niiro, Hiroaki, et al, 1995). )
  • inflammation-related enzymes such as inducible nitric oxide synthase (iNOS) and inducible cyclooxyganase (COX-2), which are present in macrophages (Niiro, Hiroaki, et al, 1995).
  • Lactoferrin is well known to play a role in the regulation of Tumor Necrosis Factor- ⁇ (TNF- ⁇ ) or Interleukin1 (IL-1) (Yamanaka, 2003).
  • TNF- ⁇ Tumor Necrosis Factor- ⁇
  • IL-1 Interleukin1
  • Colostrum also contains Insulin-like Growth Factor-1 (IGF-1), which is known to promote overall growth and to regulate cell growth and amplification (Uruakpa et al. , 2002).
  • IGF-1 Insulin-like Growth Factor-1
  • Casein which is contained in colostrum, accounts for 3% of the whole milk component and about 80% of the milk protein, and it is known to cause allergy to our body when ingested (G. H. Docena et al, 1996). Casein is also used as a cosmetic ingredient, but studies have not been fully conducted.
  • colostrum contains exfoliating ingredients (Kwon, Yu et al. 2007), and it is also known to contain TGF-beta, which inhibits melanin pigment and causes whitening effect (Martinez-Esparza M, et al. , 1997), and colostrum contains high levels of hyaluronic acid, which are excellent moisturizing and proven (Pedersen, et al, 2014).
  • Korean Patent No. 10-0964831 proposes a cosmetic composition for promoting keratin differentiation containing colostrum as an active ingredient, wherein colostrum is used for anti-inflammatory, anti-allergic, and moisturizing effects.
  • This article describes the various usefulness of cosmetic application including the efficacy of promoting keratin differentiation of colostrum ingredients.
  • Korean Patent No. 10-1003740 discloses a novel Streptococcus thermophilus strain and fermented colostrum probiotic for swine using the same to improve the weight gain of livestock, and to proliferate harmful microorganisms such as Escherichia coli and Salmonella. It is proposed to reduce the use of antibiotics and to improve productivity by preventing bacterial diarrhea disease and inhibiting the death of livestock.
  • Korean Patent Publication No. 10-2002-0021908 is a health supplement food that fermented colostrum using lactic acid bacteria, by reducing the content of harmful microorganisms present in colostrum by fermentation method using lactic acid bacteria, various functional substances possessed by colostrum. A health supplement that can be used as is is proposed.
  • Korean Patent No. 10-0619289 discloses a method for preparing a whitening cosmetics in which colostrum of colostrum is linked to arbutin, a whitening agent, and Korean Patent Publication No. 10-2009-0071998 discloses a skin keratin having colostrum as an active ingredient.
  • a composition for promoting differentiation has been proposed, and US Patent No. 5750149 proposes a technology relating to cosmetics containing substances derived from colostrum of horses.
  • Patent Document 1 Korean Registered Patent No. 10-0964831
  • Patent Document 2 Korean Registered Patent No. 10-1003740
  • Patent Document 3 Korean Patent Publication No. 10-2002-0021908
  • Patent Document 4 Korean Registered Patent No. 10-0619289
  • Patent Document 5 Korean Patent Publication No. 10-2009-0071998
  • Patent Document 6 US Patent No. 5,750,149
  • the present invention solves the problems of the prior art as described above, based on the results of the study of the availability of colostrum component, to prepare a high-functional fermentation product by fermenting colostrum component with lactic acid bacteria as a natural component.
  • an object of the present invention is to provide a colostrum fermentation product of a new composition in which colostrum is fermented with lactic acid bacteria.
  • Another object of the present invention is to provide a use of colostrum lactic acid bacteria fermentation products as anti-inflammatory and skin irritation cosmetics.
  • Another object of the present invention to provide a method for producing a colostrum fermentation product of a new composition by fermenting colostrum component with lactic acid bacteria.
  • Another object of the present invention is to provide a use of the fermented product of colostrum as a cosmetic composition effective for skin whitening, moisturizing, wrinkle improvement and the like.
  • Another object of the present invention is to provide a fermented product of colostrum as a food composition and a pharmaceutical composition for skin whitening, moisturizing, or wrinkle improvement.
  • the present invention is colostrum Lactobacillus vulgaris ( Lactobacillus delbrueckii subsp . bulgaricus ) and L actobacillus rhamnosus GG ) provides a fermentation product of colostrum, containing one or more of the lactic acid bacteria, or Lactobacillus ( Lactobacillus and Streptococcus ) mixed with lactic acid bacteria including a mixed lactic acid bacteria mixed ( Streptococcus ).
  • the present invention is the step of quenching colostrum and then pasteurized to prepare a fermentation raw material; It provides a method for producing a colostrum lactic acid bacteria fermentation product comprising the step of inoculating and fermenting lactic acid bacteria to the fermentation raw material obtained in the step.
  • the present invention further comprises the step of obtaining a fermentation extract using the fermentation product enzyme; It provides a method for producing a colostrum lactic acid bacteria fermentation product comprising a.
  • the present invention provides a cosmetic composition for anti-inflammatory and skin irritation relief comprising a specific colostrum fermentation product or lactic acid bacteria fermentation product.
  • the present invention provides a composition for skin whitening of colostrum fermentation products containing the fermentation product of colostrum obtained by fermenting colostrum as an active ingredient for skin whitening.
  • the present invention also provides a composition for moisturizing colostrum fermentation products containing the fermentation product of colostrum obtained by fermenting colostrum as an active ingredient for skin moisturizing.
  • the present invention also provides a composition for improving wrinkles of a colostrum fermentation product containing the fermentation product of colostrum obtained by fermenting colostrum as an active ingredient for improving wrinkles of the skin.
  • the present invention also provides an anti-inflammatory and skin irritant, skin whitening, moisturizing or anti-wrinkle cosmetic composition, food composition or pharmaceutical composition of a colostrum fermentation product containing the fermentation product of colostrum obtained by fermenting colostrum as an active ingredient. to provide.
  • Fermentation products by fermentation of colostrum according to the present invention for example, by using a lactic acid bacterium, which is a natural component, increases the specific active ingredient contained in colostrum and further improves the extraction efficiency. It is contained.
  • the fermentation product of colostrum according to the present invention is simple and easy to manufacture, and is high in stability since it uses natural microorganisms conventionally used in food production.
  • the colostrum fermentation product obtained by the lactic acid bacteria fermentation is very excellent anti-inflammatory effect compared to the existing shows an increased anti-inflammatory effect and skin irritation relief effect.
  • the colostrum fermentation product of the present invention is a cosmetic or food composition for skin whitening, moisturizing or wrinkle improvement. And as a pharmaceutical composition has a useful effect.
  • FIG. 1 is a process diagram illustrating an example of the manufacturing process of the lactic acid bacteria fermented colostrum extract applied in the embodiment as a fermentation product of colostrum according to the present invention.
  • Figure 2 is an experimental example of the fermentation product of colostrum according to the present invention, a graph showing the experimental results for the anti-inflammatory effect of the lactic acid bacteria fermented colostrum extract prepared in Example.
  • Figure 3 is an experimental example of the fermentation product of colostrum according to the present invention, a graph showing the experimental results for the skin irritation relaxation effect of the aerobic fermentation product of colostrum prepared in Example.
  • Figure 4 is an experimental example of the fermented product of colostrum according to the present invention, the result of the tyrosinase activity inhibition test for the colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Example and the whitening effect This is a graph showing the comparison.
  • Figure 5 is an experimental example of the fermented product of colostrum according to the present invention, a graph showing the comparative experimental results for the antioxidant effect (DPPH) for the colostrum fermented product and milk, milk fermented product and colostrum prepared in the embodiment.
  • DPPH antioxidant effect
  • FIG. 6 is an experimental example of the fermented product of colostrum according to the present invention, as a comparative experiment result for the cytotoxic effect on the colostrum fermented product and milk, milk fermented product and colostrum prepared in Example, by concentration of each sample 12 hours cell viability is a graph showing the measurement results through the MTT assay.
  • Figure 7 is an experimental example of the fermented product of colostrum according to the present invention, the result of the AQP3 mRNA expression test using a Keratinocyte for the colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Example (Aquaporin experiment) results As a result, it is a graph showing the results of comparative experiments for the measurement of mRNA expression by concentration of each sample.
  • FIG. 8 is a real-time PCR photograph of cDNA for each sample of FIG. 6.
  • FIG. 8 is a real-time PCR photograph of cDNA for each sample of FIG. 6.
  • FIG. 9 is an experimental example of the fermented product of colostrum according to the present invention, a graph showing the results of comparative experiments for the antioxidant efficacy (confirmation of SOD production) of the colostrum fermented product and milk, milk fermented product and colostrum prepared in Example to be.
  • FIG. 10 is a test example of the fermentation product of colostrum according to the present invention, a graph showing the comparative experiment results for the colostrum fermentation product and milk, milk fermentation product and colostrum prepared in Example for the moisturizing effect by measuring the moisture loss amount to be.
  • FIG. 11 is an experimental example of the fermented product of colostrum according to the present invention, comparative experiments on the effect of inhibiting the production of prostaglandin (Prostaglandin E 2 ) for the colostrum fermented product and milk, milk fermented product and colostrum prepared in Example The graph shown.
  • Prostaglandin E 2 prostaglandin
  • the present invention relates to a fermentation product of colostrum of a new composition obtained by fermentation with a specific lactic acid bacteria as a fermentation product having excellent utility obtained from colostrum.
  • Fermentation products obtained by the lactic acid bacteria fermentation of colostrum obtained in the present invention is excellent in anti-inflammatory activity, in order to replace and supplement the conventional chemical synthetic cosmetic composition, when applied to the production of natural cosmetic composition through lactic acid bacteria fermentation effect and skin irritation relief It can be provided as a new cosmetic composition with enhanced effect.
  • the present invention starts from the use of colostrum as a fermentation raw material to increase its utility.
  • the colostrum Lactobacillus vulgaris Lactobacillus Lactobacillus ( Lactobacillus ) Lactobacillus delbrueckii subsp . bulgaricus ) or L actobacillus rhamnosus It is possible to provide colostrum lactic acid fermented products fermented with lactic acid bacteria, including GG ).
  • colostrum can provide a fermentation product of colostrum, fermented with lactic acid bacteria including lactic acid bacteria mixed Lactobacillus and Streptococcus ( Streptococcus ).
  • the colostrum fermentation product is, for example, Lactobacillus streptococcus thermophilus filtered the lysate obtained by fermenting colostrum with Streptococcus thermophilus and Lactobacillus (Lactobacillus) Colostrum fermentation dissolution filtrate may be preferably used.
  • colostrum Lactobacillus delbrueckii subsp . bulgaricus ), L actobacillus rhamnosus GG provides a colostrum lactobacillus fermentation product, fermented with a lactic acid bacteria including a complex lactic acid bacteria mixed with at least one lactic acid bacteria selected from Streptococcus , more preferably Streptococcus thermophilus Can be.
  • such colostrum lactic acid bacteria fermentation products have excellent effects that could not be anticipated, such as anti-inflammatory and skin irritation relief, skin veins, skin moisturizing, wrinkle improvement.
  • the present invention includes a new use of fermentation products of colostrum having a new composition obtained by fermenting colostrum into a fermentable bacterium, not limited to lactic acid bacteria as a fermentation product having excellent usability obtained from colostrum.
  • Fermentation products obtained by the fermentation of colostrum obtained in the present invention is excellent in anti-inflammatory and skin irritation, whitening activity, skin moisturizing, wrinkle improvement, etc. in order to replace and supplement the conventional chemical synthetic cosmetic composition, through fermentation of colostrum
  • When applied to the production of natural cosmetic compositions and the like can be provided as a new cosmetics, food compositions or pharmaceutical compositions, particularly to enhance the effects of anti-inflammatory and skin irritation, whitening activity, skin moisturizing, wrinkle improvement and the like.
  • the present invention starts from the use of colostrum as a fermentation raw material to increase its utility.
  • a functional composition of colostrum fermentation product obtained by fermenting colostrum with fermentation bacteria.
  • a composition that is effective in anti-inflammatory and skin irritation, skin whitening, skin moisturizing, wrinkle improvement and the like can be provided.
  • the fermentation bacteria for fermenting colostrum for example, probiotics or lactic acid bacteria
  • specific examples thereof include Lactobacillus delbrueckii subsp.bulgaricus, Lactobacillus rhamno Lactobacillus rhamnosus GG, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus plantarum alactocillus plantarum Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus helveticus, Lactobacillus reuteri, etc.
  • Bifidobacterium bifidum Bifidobacterium bifidum Bifidobacterium longum, Bifidobacter Bifidobacterium lactis, Bifidobacterium breve, Bifidobacterium animalis, and the like can also be used.
  • Streptococcus bacterium Streptococcus thermophilus
  • Enterococcus, Lactococcus, etc. may be used.
  • the fermentation product of colostrum may include any one or more of fermentation broth obtained by fermenting colostrum with fermentation bacteria, fermentation broth removed casein, and sterilized fermentation broth.
  • the colostrum fermentation product may be preferably fermented with a combination of probiotics or lactic acid bacteria and other fermentation bacteria.
  • fermentation with lactic acid bacteria can obtain a desirable result.
  • the colostrum fermentation product may be more preferably fermented into the above-mentioned lactic acid bacteria or complex bacteria of lactic acid bacteria and other fermentation bacteria.
  • Colostrum used as a fermentation raw material in the present invention can be preferably used salty milk within three days after delivery.
  • colostrum used as a fermentation raw material may preferably use bovine colostrum, where colostrum is a concept including colostrum of domestic animals such as goats and goats, but is not limited thereto.
  • the colostrum in order to use colostrum, for example, the colostrum is rapidly cooled and then pasteurized to prepare fermented raw material.
  • Pasteurization in the present invention can be carried out by a conventional pasteurization method.
  • the fermentation material obtained in the above step may be inoculated and fermented with lactic acid bacteria to obtain a fermentation broth.
  • lactobacillus fermentation is Lactobacillus vulgaris ( Lactobacillus delbrueckii) subsp . bulgaricus ), L actobacillus rhamnosus GG ) It is preferable to use one or more lactic acid bacteria selected from the group or complex lactic acid bacteria in which Streptococcus thermophilus is mixed. The selective use of such specific lactic acid bacteria can be an important factor, for example, in achieving the desired purpose for achieving synergistic effects.
  • Lactobacillus Bulgaria kusu (Lactobacillus delbrueckii subsp. Bulgaricus) are D- lactic acid from the fermentation process (D-Lactic acid) by a production represents a detoxification role and anti-inflammatory effects, effects to improve the anti-wrinkle and skin texture Can be expressed.
  • L actobacillus rhamnosus GG is epithelialization material given to help eczema prevention, eczema can exhibit an effect in the prevention and Gram-positive bacteria is Staphylococcus aureus (staphylococcus aureus), or impede the growth perched on dead skin cells, keratinocyte migration and cell reproduction ( can contribute to re-epithelialization).
  • Lactobacillus Lactobacillus
  • Streptococcus thermophilus may have a desirable effect on the skin, such as the formation of the stratum corneum layer structure or increase the amount of ceramide necessary for this function.
  • Lactobacillus rhamnosus GG and Streptococcus thermophilus is a synergistic effect by the use of the mixture to produce Interleukin-10 (Interleukin-10), excellent anti-inflammatory Can be effective.
  • lactic acid bacteria when using the lactic acid bacteria as described above, due to the use of these lactic acid bacteria will exhibit a synergistic effect to enhance the anti-inflammatory effect with lactoferrin of colostrum.
  • the skin whitening activity can be greatly increased through fermentation of colostrum, and when using some specific bacteria or various bacteria in combination, the skin whitening effect can be further enhanced together with TGF-ß of colostrum. May have a synergistic effect;
  • some of the bacteria that can be applied to colostrum fermentation in the present invention may contribute to the skin moisturizing and skin psoriasis treatment effect by itself.
  • Lactobacillus rhamnosus GG has eczema prevention and can express the effect on atopy prevention
  • Gram-positive bacteria is Staphylococcus aureus (staphylococcus aureus) re-epithelialization (re hinder the growth perched on dead skin cells, or given aid in keratinocyte migration and cell reproduction can express effects contributing to -epithelialization.
  • Streptococcus thermophilus may have a desirable effect on the skin, such as the formation of the stratum corneum layer structure or increase the amount of ceramide necessary for this function.
  • skin fermentation and skin psoriasis treatment activity can be greatly increased through fermentation of colostrum, and in the case of using a combination of some specific bacteria or various bacteria, the effect of skin moisturizing and psoriasis along with lactoferrin of colostrum May have a synergistic effect.
  • Lactobacillus delbrueckii used as lactic acid bacteria subsp . bulgaricus ) and Streptococcus thermophilus can provide a more favorable synergistic effect for mixed use in that both bacteria provide polyamines to protect themselves from oxidative stress.
  • a composite lactic acid bacteria mixed Lactobacillus ( Lactobacillus ) and Streptococcus ( Streptococcus ) is preferably induce the production of lactic acid and antibacterial, anti-inflammatory effect And the whitening, moisturizing, wrinkles, such as the effect of the remarkably enhances the synergistic effect can be expected.
  • Lactobacillus Bulgaria kusu Lactobacillus delbrueckii subsp. Bulgaricus
  • Lactobacillus ramno suspension support Lactobacillus rhamnosus GG
  • Streptococcus thermophilus Streptococcus thermophilus
  • fermentation by fermentation bacteria or more preferably fermentation by lactic acid bacteria or complex bacteria with lactic acid bacteria inoculated with the lactic acid bacteria as described above in the pasteurized colostrum which is a fermentation raw material and at a temperature of 30 ⁇ 50 °C It can be fermented for 30 to 120 minutes.
  • the fermentation product or lactic acid bacteria fermentation product of colostrum can be obtained by centrifuging the fermentation broth.
  • the fermentation product or lactic acid bacteria fermentation product of the colostrum of the present invention may be the casein is removed.
  • the fermentation product or lactic acid bacteria fermentation product of colostrum may preferably further comprise the step of obtaining a fermentation extract using the fermentation broth enzyme.
  • the step of obtaining the fermentation extract by using the fermentation broth is preferably an agglomeration process for purifying the colostrum fermentation broth after the fermentation process.
  • the fermentation broth may be agglomerated using an enzyme to obtain a fermented product or colostrum fermented colostrum extract of colostrum.
  • the functional component content can be increased by removing those that may have a negative effect such as casein contained in the fermentation broth obtained by the lactic acid bacteria fermentation, or by removing the high-casein in milk protein. Therefore, the enzyme is added to remove the casein and other components through the aggregation reaction.
  • after the addition of the enzyme may further comprise a purification process comprising the step of centrifuging and filtering the enzyme additive.
  • fermented products of colostrum can be obtained in the form of lactic acid bacteria fermented colostrum extract, which is a more refined fermented product.
  • an enzyme complex for the role of a coagulant may be used, and specifically, for example, rennet may be preferably used.
  • Rennet illustrated herein refers to an enzyme complex in the stomach of a ruminant animal.
  • rennet is a coagulase containing rennin, one of the proteolytic enzymes, and it is known to be contained in the calf's gastric juice with pepsin. It is known that it has a characteristic of precipitating, and cheese that is aged shortly using bovine gastrointestinal enzyme rennet has a good aroma and a smooth mouth feel.
  • Enzymes used in the present invention can be understood to include all enzymes exhibiting properties similar to the aforementioned rennet.
  • an enzyme represented by such a rennet when used, it can be preferably used to cause the aggregation of casein contained in lactic acid bacteria fermented colostrum to remove casein.
  • the fermentation broth of colostrum obtained after the lactic acid bacteria fermentation may be aggregated for 30 to 120 minutes after the enzyme is added at 30 to 40 ° C.
  • the enzyme addition process may be further subjected to the aggregation process for components such as casein and then centrifuged and filtered to recover the fermentation broth.
  • the lactic acid bacteria fermented product of colostrum which is substantially free of casein components, can be obtained.
  • the lactic acid bacteria fermented product of colostrum thus obtained may be prepared in the form of lactic acid bacteria fermented colostrum extract from which casein has been removed.
  • the fermentation product of colostrum may be prepared by further filtration and sterilization.
  • the lactic acid bacteria fermented colostrum extract obtained according to the present invention may be used by removing bacteria that may be present after filtration twice or several times.
  • Lactic acid bacteria fermented colostrum extract obtained through the above steps according to the present invention is a preferred example of the fermentation product of colostrum, it was confirmed to exhibit very useful properties different from any components derived from colostrum in the past.
  • the fermentation product or lactobacillus fermentation product of colostrum according to the present invention is a fermentation broth obtained by fermenting colostrum into a specific lactic acid bacterium, a fermentation broth substantially free of casein, a sterilized fermentation broth, a fermentation product from which the fermentation broth is purified or a fermentation colostrum fermentation colostrum. It may include any one or more of the.
  • An example of the most preferred type of fermentation product of colostrum according to the present invention may be a purified lactic acid bacteria fermented colostrum extract that is sterilized without casein by purifying the fermentation broth.
  • the colostrum by treating the colostrum with a specific lactic acid bacteria as described above, it can exhibit an excellent effect unlike the prior art, in particular when using a combination of lactic acid bacteria and other bacteria of the present invention exhibits a synergistic effect Therefore, it was confirmed that the activity was significantly superior to that of the use of a single lactic acid bacterium.
  • Representative combinations of lactic acid bacteria and Streptococcus bacteria can yield surprising results with excellent synergistic effects.
  • the fermented product or lactic acid bacteria fermented product of colostrum according to the present invention has an effect on useful effects such as inhibition of cytokine generation, anti-inflammatory activity, whitening, moisturizing and wrinkle improvement by lactic acid bacteria fermentation.
  • lactic acid bacteria fermented colostrum extract has an effect on useful effects such as inhibition of cytokine generation, anti-inflammatory activity, whitening, moisturizing and wrinkle improvement by lactic acid bacteria fermentation.
  • the purification process is carried out, by eliminating the disadvantages of casein and increasing the content of functional ingredients, it is possible to simultaneously obtain the effects of anti-inflammatory, skin irritation, whitening, moisturizing and wrinkle improvement, which have more desirable results. It is possible to show an excellent synergy effect that could not be predicted before.
  • the present invention in order to replace and supplement the chemical synthetic cosmetic composition is a cosmetic that is used in the past, by preparing a natural cosmetic composition using the fermentation product of acetic acid according to the present invention skin irritation, skin irritation relief, whitening, moisturizing And the provision of new cosmetic compositions having enhanced effects such as wrinkle improvement.
  • the cosmetic composition containing the fermented product of colostrum of the present invention in the case of the cosmetic composition containing the fermented product of colostrum of the present invention, it is possible to inhibit the occurrence of inflammation-induced satokine and promote the expression of interleukin-10, thereby significantly increasing It can have anti-inflammatory and skin stimulating effects.
  • the fermentation products of colostrum such as colostrum fermentation extract according to the present invention has been shown to express tyrosine inhibitory activity, that is, melanin production inhibitory effect by the fermentation of colostrum.
  • Melanin is a generic term for brown or black polymer pigments in which phenols are oxidized by oxidase to absorb light in vivo.
  • Tyrosine a precursor of melanin
  • melanin is synthesized by the action of an enzyme called Tyrosinase.
  • Tyrosinase is an enzyme involved in the most important initial rate determining step in the melanin biosynthesis pathway in the human body, and the colostrum fermentation product according to the present invention has an amazing effect of inhibiting the Tyrosinase enzyme.
  • the fermented product of colostrum may exhibit a more excellent skin whitening effect than the milk fermented product or colostrum by increasing the content of the functional ingredient and at the same time, while eliminating the disadvantages caused by casein and the like during the purification process.
  • the colostrum fermentation product improves the skin environment by various components contained in the colostrum fermentation product while exhibiting substantial skin whitening activity against melanin production inhibition.
  • it can be used as a cosmetic for skin whitening because it can create a skin environment so that pigmentation does not occur on the skin with natural cosmetics.
  • the use of the skin whitening composition of the colostrum fermentation product according to the present invention as a cosmetic is a natural cosmetic of a new concept that is not known.
  • the cosmetic composition for whitening such colostrum fermentation products may inhibit the Tyrosinase enzyme causing melanin production, thereby exhibiting a very whitening effect of the skin.
  • the fermented product of colostrum such as colostrum fermented extract according to the present invention
  • anti-inflammatory activity by the fermentation of colostrum has a surprising effect showing the effect on the skin moisturizing and psoriasis treatment.
  • the colostrum fermentation product is able to exhibit an excellent skin moisturizing effect that cannot be predicted, in particular, compared to milk fermentation product or colostrum by increasing the content of functional ingredients while eliminating disadvantages caused by casein and the like during the purification process.
  • Psoriasis is a disease with the formation of distinct and variable erythematous papules and plaques, covered with silver-white scales on the skin, characterized by overlying hyperplasia of the epithelium and repeated chronic deterioration and improvement. It is a skin disease.
  • the cause of psoriasis is generally considered to be the cause of psoriasis, but genetic factors, environmental deterioration or triggers, and immunological factors are not clear.
  • psoriasis is a genetic factor, the individual life and environmental factors act as a trigger, immunological factors are known to cause the growth of keratinocytes and inflammatory reactions.
  • Exacerbations or triggers for psoriasis may be related to skin hydration, including skin trauma, infections, cold and dry climates such as winter, dry skin, stress, and medication.
  • Psoriasis usually occurs symmetrically and occurs in areas of high irritation such as the shin, elbow, knee, sacrum, scalp, and neck. Psoriasis initially develops a small, red, grainy rash on the skin that grows larger. A new millet-like rash develops around it, which also merges with each other as it grows. In psoriasis, white scales, such as white scales, appear piled up, and the removal of the scale has a problem of bleeding.
  • the colostrum fermentation product of the present invention unlike conventional milk or colostrum, has excellent anti-inflammatory and antimicrobial effects, and also shows activity against acne-causing bacteria and moisturizing effects. Therefore, by improving the skin environment by the various components contained in the colostrum fermentation product while showing a practical moisturizing effect and psoriasis treatment activity, it can be suitably used as a natural moisturizing cosmetic composition.
  • the cosmetic composition for moisturizing colostrum fermentation products according to the present invention is a natural concept of a new concept that is not known.
  • the composition for skin moisturizing the colostrum fermentation product is an effective ingredient useful for the treatment of psoriasis.
  • the colostrum fermentation product may exhibit an excellent anti-wrinkle effect than the milk fermentation product or colostrum by increasing the content of the functional ingredient at the same time, while eliminating the drawbacks due to casein and the like during the purification process.
  • Wrinkles begin from aging of the dermis, and the curvature of the papilla, the boundary between the epidermis and the dermis, expands and loses adhesion to elastin fibers, which are elastic fibers, changes the structure of elastin and causes the skin to fold and wrinkles. do. To form a single wrinkle, you need loose skin. During the aging process, the skin is stretched and certain physical changes appear over time.
  • the theory of aging is the free liberal theory by active oxygen theory. More than 95% of the oxygen consumed by the human body is reduced to water by combining with electrons generated during metabolism of cells, but 2 ⁇ 3% of oxygen causes cell destruction during free radical process to absorb electrons by incomplete reduction. This is called active oxygen.
  • active oxygen acts on the cell membrane composed of lipids and proteins, lipid peroxidation is induced and the content of final product Malondialdehyde (MDA) is increased.
  • MDA Malondialdehyde
  • MDA chemically modifies Low Density Lipoprotein (LDL) in the lining of the blood vessel wall, and the modified low density lipoprotein forms synthetic foams by newly synthesizing cholesterol in macrophages, forming foam cells. Aging occurs because it degrades physiological function.
  • LDL Low Density Lipoprotein
  • Vivo include the radicals oxide material according to aging (O 2 -, OH -) , the blood concentration, such as nitric oxide (NO), hydrogen peroxide (H 2 O 2) are move up the aging of the different diseases and the cell is promoted .
  • Free radicals cause damage to skin cells. Too much free radicals can break down the body's antioxidant defenses, resulting in damage to cellular components such as proteins, lipids, and DNA, altering cellular function, and ultimately promoting skin aging. Free radicals also damage connective tissue. Free radicals act on collagen metabolism and destroy collagen directly, leading to collagen deficiency. Humans are constantly exposed to the threat of free radicals. Antioxidant activity that inhibits free radicals contributes to wrinkle improvement by inhibiting free radicals and oxidizing substances.
  • the colostrum fermentation product of the present invention unlike conventional milk or colostrum, is excellent in anti-inflammatory, antibacterial and antioxidant effects, and also shows a moisturizing effect. Therefore, such colostrum fermentation products can be suitably used as a cosmetic composition for improving wrinkles by improving the skin environment by various ingredients contained in colostrum fermentation products while exhibiting excellent activity for substantial wrinkle improvement.
  • the lactic acid bacteria fermentation product of colostrum in the cosmetic composition may be contained in an amount of 0.01 to 40% by weight based on the total weight of the composition. Outside the lower limit of the above content range, the effect is remarkably reduced. Outside the upper limit, it is difficult to prepare a cosmetic formulation, or it is not preferable in terms of economics.
  • the lactic acid bacteria fermented product of colostrum prepared by the method as described above may be added to the cosmetic composition, for example, as a lactic acid bacteria fermented colostrum extract, for example, powdery, liquid, nanocapsules, complex It may be added in the form of, and the like, and may be added in a liquid phase as a preferred example.
  • a lactic acid bacteria fermented colostrum extract for example, powdery, liquid, nanocapsules, complex It may be added in the form of, and the like, and may be added in a liquid phase as a preferred example.
  • a cosmetic composition containing lactic acid bacteria fermentation product of colostrum can be used in cosmetic formulations such as lotion (skin lotion), nutrition lotion, nutrition cream, massage cream, nutrition essence, cleanser, mask pack, and the like. It is not limited, and may be used in other hair preparations or skin preparations for various functional effects.
  • the lactic acid bacteria fermented product of colostrum contained in the cosmetic composition prepared according to the present invention is the raw material used in the manufacturing process is colostrum and the lactic acid bacteria used to ferment it is a natural microorganism that has conventionally been used in food production, and thus stability of the extract itself.
  • the lactic acid bacteria used to ferment it is a natural microorganism that has conventionally been used in food production, and thus stability of the extract itself.
  • it can exhibit a remarkably excellent skin inflammatory and skin irritation effect, and is expected to be very useful as a new concept cosmetic composition due to the effects of whitening, moisturizing, wrinkle improvement and the like.
  • the raw material is colostrum and the fermentation bacterium used to ferment it is a natural microorganism that has been conventionally used in food production, and thus has high safety for the extract itself, and alleviates skin irritation and skin irritation. It is superior to milk, fermented milk, colostrum, etc. in the effects of skin, whitening, moisturizing and wrinkle improvement.
  • colostrum fermented products fermented with colostrum but using lactic acid bacteria and other microorganisms are more effective in anti-inflammatory, skin irritation, whitening, moisturizing, and wrinkle improvement. great.
  • the cosmetic composition applied for the purpose of anti-inflammatory, skin irritation, whitening, moisturizing, wrinkle improvement and the like of colostrum fermentation products according to the present invention when applied to the skin as a cosmetic or as a pharmaceutical composition for example skin ointment
  • a cosmetic or as a pharmaceutical composition for example skin ointment
  • skin irritation, skin irritation relief, whitening, moisturizing, wrinkle improvement, psoriasis treatment and the like can be expected to be very useful as a new concept cosmetic composition or pharmaceutical composition.
  • the composition obtained as the fermentation product of colostrum may be applied as a food composition.
  • it can be applied as a health functional food for anti-inflammatory, skin irritation, whitening, moisturizing, wrinkle improvement and psoriasis treatment.
  • the colostrum fermentation product of the present invention may be specifically applied to the skin several times at 1-500mg / ml.
  • This form can also be prepared by constructing 1-500mg / ml even when applied to the cosmetics applied to the anti-acne cosmetics applied to the skin.
  • more preferably 10-400 mg / ml can be applied.
  • cosmetics using a moisturizing cosmetic composition containing a colostrum fermentation product according to the present invention as an active ingredient skin ointment containing a colostrum fermentation product as an active ingredient within the above content range, for example 50mg / ml, 100mg / ml, 200mg / ml, 400mg / ml and the like can be prepared and applied in various contents.
  • the health food or pharmaceutical composition according to the present invention can be orally administered 1-3 times a day, such as 50 mg, 100 mg, 200 mg, 400 mg, 500 mg or 1,000 mg once as an oral agent.
  • the effect is remarkably reduced, and when the colostrum fermentation product exceeds the upper limit, it is difficult to prepare a composition or formulation for skin application, The expected effect does not improve significantly, which is undesirable from an economic point of view.
  • the colostrum fermentation products as described above can be used as cosmetics having uses such as anti-inflammatory, skin irritation relief, whitening, moisturizing, wrinkle improvement, etc. It may be applied as a food composition or pharmaceutical composition such as skin ointment.
  • the fermented colostrum extract was prepared by the same process as in the process of FIG.
  • Step 1 prepare raw materials
  • Second step production of lactic acid bacteria fermentation broth
  • Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842), Streptococcus thermophilus ( ATCC 19258), L actobacillus rhamnosus GG , ATCC 53103) three kinds of complex bacteria were inoculated into the fermentation raw material of the first step and fermented at a temperature of 35 °C 60 minutes to obtain a fermentation product (fermentation liquid).
  • Step 3 Preparation of Lactic Acid Bacteria Fermented Colostrum Extract
  • the fermentation broth obtained in the second step is centrifuged at 2000 rpm for 20 minutes to remove the suspended matter, and the supernatant except the precipitate is recovered to obtain the fermented colostrum as a fermentation product of colostrum.
  • the fermented colostrum is adjusted to 33 ° C., and agnetted for 60 minutes by adding Rennet to recover the fermented colostrum.
  • the fermented colostrum obtained above was centrifuged at 13000 rpm for 60 minutes to obtain a supernatant as a fermented colostrum extract that is a lactic acid bacteria fermentation product.
  • the supernatant was secondly filtered with a filter paper of 0.4 ⁇ m and 0.2 ⁇ m, and then the lactic acid bacteria fermented colostrum extract was prepared as a fermentation product of colostrum as a final lactic acid bacteria fermentation product.
  • the fermented milk product was prepared in the same manner as in Example 1 by adjusting the bacteria or complex bacteria inoculated into the fermentation raw material of the first step in Example 1 as shown in Table 1.
  • Example 2 Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842) Example 3 L actobacillus rhamnosus GG , ATCC 53103) Example 4 Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842) + L actobacillus rhamnosus GG , ATCC 53103) Example 5 Lactobacillus delbrueckii subsp . bulgaricus , ATCC 11842) + Streptococcus thermophilus , ATCC 19258 Example 6 L actobacillus rhamnosus GG , ATCC 53103) + Streptococcus thermophilus , ATCC 19258
  • the fermentation product of colostrum was prepared in the same manner as in Example 1, using Streptococcus thermophilus ( ATCC 19258) alone as a bacterium used for fermentation.
  • Lactobacillus Bulgaria kusu same as in the method of Example 2, except that Figure the Lactobacillus ash instead of (Lactobacillus delbrueckii subsp. Bulgaricus, ATCC 11842) using the filler's (Lactobacillus acidophilus, ATCC 4356) It carried out by the method, the lactic acid bacteria fermented colostrum extract was prepared.
  • Lactobacillus Bulgaria kusu Lactobacillus delbrueckii subsp. Bulgaricus, ATCC 11842
  • Streptococcus Thermo filler's Streptococcus thermophilus, ATCC 19258
  • Lactobacillus fermented colostrum extract was prepared in the same manner as in Example 6 except for using Streptococcus thermophilus ( ATCC 19258).
  • LPS Lipopolysaccharide
  • Activated macrophages are TNF- ⁇ , an inflammation-inducing cytokine. Increase the expression levels of IL-1 ⁇ , IL-12, IFN ⁇ and the like. Inflammatory cytokines produce Nitric oxide (NO). Therefore, the anti-inflammatory effect was evaluated by reducing the amount of NO expression.
  • RAW 264.7 cells were suspended in DMEM (Dubelccos Modified Eagle Medium, Gibco) animal cell culture medium containing 10% FBS, each containing 5 X 10 5 per 24 well plate at 37 ° C., 5% CO 2 conditions. Incubate for one day at. Thereafter, the medium was washed twice and the sample was prepared, and then the LPS was mixed with the prepared sample at a concentration of 1 ⁇ g / ml and treated at the same time in a cell at 37 ° C. in a 5% CO 2 incubator for 18 hours.
  • DMEM Dubelccos Modified Eagle Medium, Gibco
  • a patch test was performed on the forearm of 20 adult men and women (10 males and 10 females) in their 20s. Skin irritation was used 0.1% by weight of sodium lauryl sulfate (SLS), group A fermented SLS only, group B fermented SLS + non-fermented colostrum extract, group C fermented SLS + Streptococcus thermophilus ( ATCC 19258) Colostrum extract, Group D, as fermented colostrum extract (Example 1), each was patched to the skin for one day. After the patch was finished, the test site was marked with a pen, and after 24 hours, the skin reaction of the test site was determined according to the criteria of Table 3 below.
  • SLS sodium lauryl sulfate
  • group A fermented SLS only group B fermented SLS + non-fermented colostrum extract
  • group C fermented SLS + Streptococcus thermophilus ATCC 19258
  • Colostrum extract Group D, as fermented colostru
  • Melanin is a generic term for brown or black polymer pigments in which phenols are oxidized by oxidase and are widely distributed in the flora and fauna and play a role in absorbing light in living bodies.
  • Tyrosine a precursor of melanin
  • melanin is synthesized through Dopa and Dopaquinone through the action of an enzyme called Tyrosinase.
  • Tyrosinase is an enzyme involved in the most important initial rate determining step in the melanin biosynthetic pathway in the human body, and many whitening components have a mechanism of action to inhibit this enzyme.
  • the whitening efficacy was evaluated by evaluating the degree of inhibition of Tyrosinase enzyme activity in the in vitro colostrum fermentation product (fermented colostrum).
  • test method add 220ul of 0.1M phosphate buffer solution, 20ul of sample solution and 20ul of mushroom tyrosinase (1500U ⁇ 2000U / ml) solution. 40ul of 1.5mM tyrosine solution was added to this solution and reacted at 37 ° C for 10-15 minutes. Then, the absorbance is measured at 492 nm using an ELISA reader. Add 0.1M phosphate buffer solution instead of sample solution.
  • a represents absorbance after the reaction of the sample solution
  • b represents absorbance after the reaction of the sample solution
  • a 'and b' mean absorbance measured by replacing the buffer with tyrosinase.
  • the colostrum extract was reacted with DPPH in vitro, and the degree of free radical reduction was measured by ELISA to measure antioxidant activity.
  • This colostrum fermentation product showed a markedly improved effect as shown in Table 7 below compared with other fermented milk fermentation or colostrum.
  • the MTT assay is a method widely used for cell proliferation and toxicity by measuring the number of living cells.
  • the living cells are reduced in blue formazan derivatives which are water-soluble in the mitochondria and yellow salt MTT is water-insoluble by succinate dehydrogenase. Is to use the principle.
  • the 6-well plate was inoculated with HaCaT cells at a density of 1 ⁇ 10 5 cells / well and incubated at 37 ° C. for one day. Thereafter, each well was treated with each sample (milk, milk fermentation, colostrum, colostrum fermentation) by concentration (1,10,20ug / ml). After 12 hours of incubation at 37 °C treated with MTT solution and activated for 1 hour was measured at 450nm with ELISA reader. The control proceeded with H 2 O 2 and showed cell viability compared to the control group.
  • AQP0-AQP12 aquaporins
  • skin-related cells such as human keratinocytes, melanocytes, skin fibroblasts
  • AQP3 is found in Epidermis and serves to permeate both water and glycerol.
  • AQP3 deletion shows normal skin structure, but it has the effect of reducing the stratum corneum's water content and water and glycerol epidermal permeability. Accordingly, the moisturizing effect was confirmed using the AQP3 mRNA expression amount.
  • the keratinocytes were divided into 4x10 ⁇ 5cells / well in 6 well plates, and incubated for 24 hours, and then each sample (milk, milk fermentation, colostrum, colostrum fermentation) by concentration (0, 1, 10, 100ug / ml) ). After 3 hours, cells were collected, RNA was extracted, and cDNA was synthesized. Real-time PCR was performed on the produced cDNA to measure the expression level of mRNA.
  • Superoxide dismutase is an important antioxidant enzyme that catalyzes the conversion of superoxide anion into oxygen and hydrogen peroxide.
  • WST Water Soluble Tetrazolium salt
  • O 2- superoxide anion
  • XO Xanthine Oxidase
  • HaCaT cells were inoculated in human skin cells (HaCaT) at a density of 1 ⁇ 10 5 cells / well in a 6-well plate and incubated at 37 ° C. for one day. Thereafter, no treatment (control), milk, fermented milk, colostrum, fermented colostrum were treated at concentrations of 30, 40 and 50mg / ml, respectively, and incubated for 12 hours. After reacting the cells with the WST-1 reagent for 15 minutes, the cells were measured at 450 nm with an ELISA reader.
  • the moisturizing power of the sample was measured by pouring milk, milk fermentation, colostrum and colostrum fermentation into 100 mL beaker, and weighing for 24 hours every 2 hours in a constant temperature / humidifier at 25 ° C and 50% relative humidity. Compared. Primary distilled water was used as a control.
  • Prostaglandins are known to play an important role in the inflammatory E 2 (Prostaglandin E 2, PGE 2) To investigate the inhibitory effect produced.
  • Cyclooxygenase (COX) is an enzyme that converts arachidonic acid to prostaglandins and is classified as COX-1 and COX-2.
  • COX-1 acts on normal biological functions such as platelet formation, gastric wall protection, and renal function maintenance.
  • COX-2 forms inflammatory mediator PGE 2 .
  • PGE 2 is also known to be involved in cancer, including inflammatory, immune and angiogenesis.
  • compositions and contents shown in Table 14, respectively, and then cooling them to room temperature.
  • Colostrum Fermentation Products 10.0 1,3-butylene glycol 4,0 glycerin 3.0 Oleyl alcohol 0.1 Polysorbate 20 1.0 Pasqueflower Extract 1.0 Spices a very small amount Purified water Remaining amount
  • the skin response of the test site was also determined according to the above criteria.

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Abstract

La présente invention concerne un produit fermenté de colostrum et une composition cosmétique l'utilisant. Plus spécifiquement, la présente invention concerne un produit fermenté de colostrum obtenu par fermentation de colostrum à l'aide d'un zymogène, d'un lactobacille spécifique, d'un complexe bactérien ou similaires, ainsi qu'une composition fonctionnelle de matière première utilisant ce dernier et présentant de ce fait des effets tels que des effets anti-inflammatoires, de soulagement d'irritation de la peau, de blanchiment, d'hydratation et d'atténuation des rides. Le produit fermenté de colostrum peut être adopté pour une utilisation comme composition cosmétique, composition alimentaire, composition médicamenteuse, ou similaires.
PCT/KR2018/003492 2017-03-24 2018-03-23 Produit fermenté de colostrum et composition cosmétique l'utilisant Ceased WO2018174669A1 (fr)

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CN112370390A (zh) * 2020-11-27 2021-02-19 奥克斯路株式会社 一种抗衰老、去皱、修护肌肤屏障的发酵液及其制备方法
CN112370390B (zh) * 2020-11-27 2022-06-28 奥克斯路株式会社 一种抗衰老、去皱、修护肌肤屏障的发酵液及其制备方法
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CN114262675A (zh) * 2021-12-20 2022-04-01 无锡弘焕微生态科技有限公司 复合菌发酵滤液的制备及其美白功效在化妆品中的应用
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WO2018174369A1 (fr) 2018-09-27

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