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WO2018170875A1 - Procédé de prévention et de diagnostic de maladie d'animal de compagnie fondés sur une technologie d'ancrage de gène à l'échelle nanométrique et d'agglutination immunologique microfluidique - Google Patents

Procédé de prévention et de diagnostic de maladie d'animal de compagnie fondés sur une technologie d'ancrage de gène à l'échelle nanométrique et d'agglutination immunologique microfluidique Download PDF

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WO2018170875A1
WO2018170875A1 PCT/CN2017/078061 CN2017078061W WO2018170875A1 WO 2018170875 A1 WO2018170875 A1 WO 2018170875A1 CN 2017078061 W CN2017078061 W CN 2017078061W WO 2018170875 A1 WO2018170875 A1 WO 2018170875A1
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signal
module
pet
conversion
diagnosis method
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师永生
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression

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  • the invention relates to the technical field of pet prevention and diagnosis, in particular to a pet anti-diagnosis method of gene nano anchoring and microfluidic immunoagglutination technology.
  • Pet breeding is not only related to the development of human society, but also related to economic development. Over the past 30 years of reform and opening up, China's GDP has developed at a rate of nearly 10% per year. The progress of urbanization has reached more than 50%. With the result, the size of the family has shrunk, the number of families has increased, and the family structure has taken place. Change; the problem of population aging has become increasingly prominent, and the situation of empty nesters and young people has made the urban residents' emotional support, leisure and entertainment, and consumer spending also diversified. Because "to some extent, animals are our tools to carry ideas," keeping pets has gradually become one of the ways in which young people show their individuality and the feelings of the elderly, and pets have become important members of the family.
  • Pets have become an indispensable part of the family, and the related pet prevention and diagnosis industry has undergone major changes, and the disease has become more complicated, preventive and The diversified diagnostic tools and more humane services have brought unprecedented opportunities and challenges to the entire pet prevention and diagnostic industry.
  • IMD Inherited errors of disease
  • IEM inborn errors of metabolism
  • C reactive protein is synthesized by the liver and is a typical acute phase protein. Its content in normal serum is extremely small. It is in the plasma of patients with acute inflammation, malignant tumor, ischemia, tissue damage and other diseases. Among them, the CRP content can be increased by a thousand times. It increased in the early 4h-6h of inflammation and reached a peak in 36h-50h. Once the infection is controlled, its content will quickly return to normal levels. Studies have found that bacterial infections can cause elevated levels of CRP, and when more than 140 mg/L, the presence of bacterial infections can be determined. And its level is positively correlated with the degree of infection; when the virus is infected, the CRP level is generally not elevated. For the diagnosis of acute infectious diseases, the detection of serum CRP is more sensitive, more accurate and more reliable than the total number of white blood cells.
  • PCT Procalcitonin
  • calcitonin is a precursor of calcitonin, which is a glycoprotein with a half-life of 25h to 30h, and has good stability in vitro and in vivo. It is produced by C cells of the thyroid gland under physiological conditions, but its content is extremely low (less than 0.1 ⁇ g/L). It is considered abnormal when its content is more than 0.5 ⁇ g/L.
  • PCT is a diagnostic indicator for early bacterial or fungal infections, which is elevated at the time of infection. The level of non-infection is generally not elevated, and the magnitude of the increase is closely related to the severity of the infection.
  • the object of the present invention is to provide a pet anti-diagnosis method for gene nano-anchoring and microfluidic immunoagglutination technology, which utilizes gene nano-anchoring technology to prevent and diagnose pet genetic diseases and viral infections, and can be more timely and effective for pets. Treatment will improve the cure rate of pets to a greater extent and ensure the health of pets. At the same time, through the combined application of PCT and CRP, it will be more widely used and more convenient when used alone, and the diagnosis results will be more accurate.
  • the invention provides a pet anti-diagnosis method for gene nano anchoring and microfluidic immunoagglutination technology, comprising: a microfluidic chip, a photodetector, a wireless transmission module, an interface device card inserted into a PCI PCI slot, located inside the microcomputer Software measurement and control module; further comprises a DNA sample preparation module, a surface modified ligand magnetic bead, a DNA library chip, a sequence signal acquisition and analysis module, a data display module; wherein the microfluidic chip comprises a sample injection zone, a dyeing zone, a reaction zone, CRP and PCT antibodies are respectively immobilized in the reaction zone, wherein the photodetector transmits a signal generated by the antigen-antibody agglutination reaction on the microfluidic chip to the wireless transmission module, and the wireless transmission module transmits the signal to the interface device through wireless transmission.
  • the card, the interface device card is connected to the communication between the computer and the detector, and the analog signal generated by the photodetector can be collected and transmitted to the software measurement and control module by inserting in the PCI card slot, and the software measurement and control module includes A/D conversion, Digital signal analysis processing, D/A conversion; said A/D conversion: converting two analog signals into digital signals
  • the digital signal analysis process analyzing the digital signal, treating the signal and its noise as a random signal, and using the statistical features, reducing the noise to obtain the signal itself and integrating the signal to obtain the best digital signal
  • the D /A conversion converts the best digital signal analyzed and converted into the corresponding analog signal; wherein the magnetic beads of the surface modification ligand have the same polarity, and the magnetic beads mutually repel each other, and the DNA library chip has a surface modification ligand
  • the magnetic beads match the active sites, the active sites have opposite polarities to the magnetic beads, and are adsorbed by the magnetic beads.
  • the sequence signal acquisition and analysis module collects and analyze
  • the microfluidic chip is made of glass, and the microchannel is formed by soft etching, and the CRP antibody and the PCT antibody are preliminarily fixed on the aldehyde-treated glass slide at a predetermined position and then at 37. Incubate for 1 hour at °C.
  • the digital signal analysis processing uses digital filtering, FFT analysis, single product extraction, single frequency detection, wavelet denoising, autocorrelation analysis, weighted average filtering, anti-pulse interference average filtering, and program judgment filtering. , adaptive filtering, linear neural network denoising, curve fitting methods to achieve related functions.
  • the software measurement and control module performs A/D conversion on the input analog signal by starting digital signal analysis processing, and each When a data is collected, it is sent to the digital signal analysis processing for filtering operation, and the operation result is converted into analog quantity by D/A conversion. The above process is continuously repeated, and the filtered analog signal is obtained at the output of the D/A conversion.
  • the operation process not only the signal is denoised, but also the two analog signals outputted by the series module of the conductivity cell are converted into two digital signals by A/D conversion, and further analyzed and sent to D/A. The conversion is converted to an analog quantity, which in turn further forms the data.
  • the DNA library chip comprises a pet genetic disease gene chip and a pet susceptible virus gene chip.
  • the pet susceptible virus microarray has a DNA probe with different viruses at each active site, and the pet is infected or infected with a virus, and then further determined by gene sequencing. Determine if you are infected with a virus.
  • the beneficial effects of the invention are that, through the combined application of PCT and CRP, the application range is wider and more convenient than that of the two, and the diagnosis result is more accurate, and the bacterial infection is comprehensively diagnosed, and the gene sequencing is utilized.
  • the technology prevents and diagnoses pet's hereditary diseases and infectious diseases. It can treat pets in advance, grasp the timing of treatment, and better ensure the health of pets.
  • Figure 1 is a schematic view of the structure of the present invention
  • the invention provides a pet anti-diagnosis method for gene nano anchoring and microfluidic immunoagglutination technology, comprising: a microfluidic chip, a photodetector, a wireless transmission module, an interface device card inserted into a PCI PCI slot, located inside the microcomputer Software measurement and control module; further comprises a DNA sample preparation module, a surface modified ligand magnetic bead, a DNA library chip, a sequence signal acquisition and analysis module, a data display module; wherein the microfluidic chip comprises a sample injection zone, a dyeing zone, a reaction zone, CRP and PCT antibodies are respectively immobilized in the reaction zone, wherein the photodetector transmits a signal generated by the antigen-antibody agglutination reaction on the microfluidic chip to the wireless transmission module, and the wireless transmission module transmits the signal to the interface device through wireless transmission.
  • the card, the interface device card is connected to the communication between the computer and the detector, and the analog signal generated by the photodetector can be collected and transmitted to the software measurement and control module by inserting in the PCI card slot, and the software measurement and control module includes A/D conversion, Digital signal analysis processing, D/A conversion; said A/D conversion: converting two analog signals into digital signals
  • the digital signal analysis process analyzing the digital signal, treating the signal and its noise as a random signal, and using the statistical features, reducing the noise to obtain the signal itself and integrating the signal to obtain the best digital signal
  • the D /A conversion converts the best digital signal analyzed and converted into the corresponding analog signal; wherein the magnetic beads of the surface modification ligand have the same polarity, and the magnetic beads mutually repel each other, and the DNA library chip has a surface modification ligand
  • the magnetic beads match the active sites, the active sites have opposite polarities to the magnetic beads, and are adsorbed by the magnetic beads.
  • the sequence signal acquisition and analysis module collects and analyze
  • the microfluidic chip is made of glass, and the microchannel is formed by soft etching, and the CRP antibody and the PCT antibody are preliminarily fixed on the aldehyde-treated glass slide at a predetermined position and then at 37. Incubate for 1 hour at °C.
  • the digital signal analysis processing uses digital filtering, FFT analysis, single product extraction, single frequency detection, wavelet denoising, autocorrelation analysis, weighted average filtering, anti-pulse interference average filtering, and program judgment filtering. , adaptive filtering, linear neural network denoising, curve fitting methods to achieve related functions.
  • the software measurement and control module performs A/D conversion on the input analog signal by starting digital signal analysis processing, and each time a data is collected, it is sent to the digital signal analysis processing for filtering operation, and the operation result is sent to D/A conversion. Convert to analog. The above process is continuously repeated, and the filtered analog signal is obtained at the output of the D/A conversion.
  • the operation process not only the signal is denoised, but also the two analog signals outputted by the series module of the conductivity cell are converted into two digital signals by A/D conversion, and further analyzed and sent to D/A. The conversion is converted to an analog quantity, which in turn further forms the data.
  • the DNA library chip comprises a pet genetic disease gene chip and a pet susceptible virus gene chip.
  • the pet susceptible virus microarray has a DNA probe with different viruses at each active site, and the pet is infected or infected with a virus, and then further determined by gene sequencing. Determine if you are infected with a virus.
  • the blood sample is added to the system, a part of the blood enters the microfluidic chip injection area, flows through the microchannel to the dyeing zone, and is mixed and incubated with the fluorescent dyeing solution of the dyeing zone, fully combined, and then passed through the microchannel.
  • agglutination reaction with CRP antibody and PCT antibody after the reaction is completed, using a photodetector for detection, the detection signal is transmitted to the interface device card located in the microcomputer through the wireless transmission module, and inserted into the PCI card
  • the slot can collect the analog signal generated by the photodetector, transmit it to the software measurement and control module, and obtain the required data through the software measurement and control module; another part of the blood enters the DNA sample preparation module, and after the DNA sample is prepared, the surface modification ligand is prepared.
  • the magnetic beads adsorb and adsorb the magnetic beads of the DNA sample into the DNA library chip, and combine with the active site, and the sequence signal acquisition and analysis module collects and analyzes the optical signal, and the optical signal is digitally processed and then obtained through the data display module.
  • the gene sequence to be tested The gene sequence to be tested.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biotechnology (AREA)
  • Evolutionary Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

L'invention concerne un procédé de prévention et de diagnostic de maladie d'animal de compagnie fondés sur une technologie d'ancrage de gène à l'échelle nanométrique et d'agglutination immunologique microfluidique comprenant : une puce de commande microfluidique, un détecteur photoélectrique, un module de transmission sans fil, une carte de dispositif d'interface insérée dans une fente PCI de micro-ordinateur et un module de mesure et de commande logiciel à l'intérieur du micro-ordinateur ; et comprenant en outre : un module de préparation d'échantillon d'ADN, des billes magnétiques pour ligands de modification de surface, une puce de bibliothèque d'ADN, un module de collecte et d'analyse de signaux de séquençage et un module d'affichage de données. Par comparaison avec l'utilisation séparée de PCT et de CRP, une application combinée de ces derniers permet une plus grande plage d'utilisation, une plus grande commodité et des résultats de diagnostic plus précis. De plus, un diagnostic complet est réalisé sur des infections bactériennes, et la prévention et le diagnostic sont effectués sur des maladies héréditaires et des maladies infectieuses d'animaux de compagnie au moyen d'une technologie de séquençage de gènes, de telle sorte que les animaux de compagnie puissent être traités à l'avance de symptômes, que la synchronisation du traitement soit contrôlée et que la santé de l'animal de compagnie soit assurée.
PCT/CN2017/078061 2017-03-24 2017-03-24 Procédé de prévention et de diagnostic de maladie d'animal de compagnie fondés sur une technologie d'ancrage de gène à l'échelle nanométrique et d'agglutination immunologique microfluidique Ceased WO2018170875A1 (fr)

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CN201710181783.1A CN106940759A (zh) 2017-03-24 2017-03-24 一种基因纳米锚定和微流体免疫凝集技术的宠物防诊方法
CN201710181783.1 2017-03-24

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CN108764092B (zh) * 2018-05-21 2021-09-21 湖南理工学院 基于能量元双阈值的微流控芯片信号去噪方法
CN115326789B (zh) * 2022-07-11 2025-07-15 中国科学院大学 基于便携式设备的发光免疫分析方法、装置及电子设备

Citations (3)

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CN104826677A (zh) * 2015-05-05 2015-08-12 南京闻智生物科技有限公司 一种用于急症腹痛疾病排查的免疫检测微流控芯片及其制备方法和检测方法
CN105203775A (zh) * 2015-10-26 2015-12-30 深圳华迈兴微医疗科技有限公司 一种降钙素原定量检测的磁微粒化学发光微流控芯片
US20160274098A1 (en) * 2015-03-16 2016-09-22 National Chiao Tung University Magnetic bead-based digital microfluidic immunoanalysis device and method thereof

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US20160274098A1 (en) * 2015-03-16 2016-09-22 National Chiao Tung University Magnetic bead-based digital microfluidic immunoanalysis device and method thereof
CN104826677A (zh) * 2015-05-05 2015-08-12 南京闻智生物科技有限公司 一种用于急症腹痛疾病排查的免疫检测微流控芯片及其制备方法和检测方法
CN105203775A (zh) * 2015-10-26 2015-12-30 深圳华迈兴微医疗科技有限公司 一种降钙素原定量检测的磁微粒化学发光微流控芯片

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