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WO2018169284A1 - Bioréacteur pour la prolifération de cellules in vitro - Google Patents

Bioréacteur pour la prolifération de cellules in vitro Download PDF

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Publication number
WO2018169284A1
WO2018169284A1 PCT/KR2018/002943 KR2018002943W WO2018169284A1 WO 2018169284 A1 WO2018169284 A1 WO 2018169284A1 KR 2018002943 W KR2018002943 W KR 2018002943W WO 2018169284 A1 WO2018169284 A1 WO 2018169284A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
bioreactor
pressure
unit
vitro proliferation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/002943
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English (en)
Korean (ko)
Inventor
신정욱
강윤경
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industry Academic Cooperation Foundation of Inje University
Original Assignee
Industry Academic Cooperation Foundation of Inje University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry Academic Cooperation Foundation of Inje University filed Critical Industry Academic Cooperation Foundation of Inje University
Publication of WO2018169284A1 publication Critical patent/WO2018169284A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

Definitions

  • the present invention relates to a bioreactor for in vitro proliferation of cells, and more particularly, to a bioreactor capable of in vitro proliferation of hematopoietic stem cells or cells having non-adherent properties obtained from a human body, and a storage unit for storing cells.
  • the present invention relates to a bioreactor for in vitro proliferation of cells that can easily proliferate hematopoietic stem cells or cells having non-adherent properties in vitro by maintaining an environment similar to the proliferation environment by applying pressure to the inside and stirring the same.
  • cord blood refers to the blood collected from the umbilical cord, which is connected to the mother's placenta and supplies nutrients and oxygen to the fetus.
  • Umbilical cord blood contains a large amount of hemopoietic stem cells that produce blood cells such as red blood cells, white blood cells, and platelets, which play an important role in maintaining the body's immune system and life. Therefore, as well as various refractory blood diseases, it is used as a valuable life resource to treat solid cancers such as breast cancer and various metabolic diseases, hereditary diseases and the like. It is commonly known as bone marrow transplant, but it is actually bone marrow hematopoietic stem cell transplant.
  • Cord blood hematopoietic stem cells may also be used in the same way, but have several advantages over bone marrow. Since hematopoietic stem cells in umbilical cord blood are immature than hematopoietic stem cells in bone marrow, cord blood hematopoietic stem cells should be transplanted when only four genetic factors are matched. This is a great advantage, since it is possible and there are fewer immunological side effects after transplantation. Umbilical cord blood is also easier to collect than bone marrow.
  • cord blood hematopoietic stem cells have less complications in transplants where the HLA genes do not match perfectly.
  • gene therapy such as its usefulness varies.
  • Hematopoietic stem cells are absolutely necessary for human survival and are usually present in the bone marrow or placenta and umbilical cord of babies.
  • cell therapy research using stem cells present in cord blood has been actively conducted, and thus the medical and social value of cord blood Is expected to spread dramatically.
  • hematopoietic stem cells are difficult to grow in vitro and difficult to maintain performance in vitro.
  • a portion of the blood bag is placed in a blood bag (bag) and then transplanted after storage for a certain period (about 72 hours).
  • Hematopoietic stem cells are unlikely to grow in vitro and difficult to maintain performance in vitro, unlike normal cells.
  • hematopoietic cells are not cells that attach and grow, but are also floating in the body and may be suspended at some positions. Therefore, in general, it is difficult to incubate by attaching in the culture plate, there was a problem that the performance is sharply reduced even if diarrhea culture.
  • the present invention to solve the above problems
  • a base portion coupled to one side of the stirring portion
  • a holder part installed vertically from one surface of the base part
  • a storage unit storing cells to be proliferated and mounted on one surface of the base unit; It provides a bioreactor for cell in vitro proliferation comprising; and a control unit for adjusting the pressure and release pressure into the reservoir.
  • the base portion of the plate-shaped plate may include a protrusion formed to project a plurality on one surface of the plate.
  • the holder portion is a pair of supports extending vertically spaced apart from each other from the plate; And a fastening portion connected between the pair of supports, wherein the height of the fastening portion is adjustable along the support.
  • the storage unit may be made of any one of a sealed pouch, a pack, and a blood bag which are generally used for blood storage.
  • the storage unit is characterized in that it comprises one tube tube generally connected to the outside.
  • control unit is connected to the storage and the pressure supply for applying pressure to the inside of the storage; And a pressure release unit connected to the pressure supply unit and releasing the pressure pressurized by the storage unit.
  • the pressure supply unit, the first tube tube one side is inserted into the storage;
  • a compressor connected to the other side of the first tube tube to apply pressure; It may include a filter unit located between the storage unit and the compressor and a solenoid valve positioned between the storage unit and the filter unit.
  • the pressure of the solenoid valve may be characterized in that 18Kpa to 22Kpa.
  • control unit may further include a control unit for automatically controlling the solenoid valve and the clamp.
  • control unit may be characterized in that the control to close the clamp when pressed to the storage unit.
  • control unit may be characterized in that the solenoid valve is closed and the clamp is opened when back pressure.
  • the storage unit for storing the hematopoietic stem cells collected from the human body by adding pressure to the storage unit for storing the hematopoietic stem cells collected from the human body and stirring the storage unit, it is possible to maintain in vitro proliferation and performance of the hematopoietic stem cells.
  • the storage portion is built up vertically or at a desired angle, so that the flow of gas and hematopoietic stem cells introduced into the storage portion Stirring can be done smoothly.
  • the reservoir by placing the reservoir on one surface of the base including a plurality of protrusions protruding on one surface of the plate, to prevent the reservoir from being separated from the base and at the same time the contact of the reservoir during stirring It gives the effect of fluctuating irregularly.
  • the solenoid valve is disposed between the first tube tube and the compressor to apply a constant hydrostatic pressure desired by the user to the reservoir, thereby maintaining the state of the hematopoietic stem cells in the reservoir for a predetermined period of time. You can.
  • FIG. 1 is a schematic perspective view of a bioreactor for in vitro proliferation of cells according to an embodiment of the present invention.
  • Figure 2 is a side view showing a shape in which the storage unit of the present invention is installed obliquely.
  • FIG. 3 is a conceptual diagram showing the configuration of a bioreactor for in vitro proliferation of cells according to an embodiment of the present invention.
  • 4 and 5 are conceptual views showing that the pressure is released and released to the reservoir.
  • Figure 6 is a schematic perspective view of a cell in vitro proliferation bioreactor according to another embodiment of the present invention.
  • FIG. 1 is a schematic perspective view of a bioreactor for in vitro proliferation of cells according to an embodiment of the present invention
  • Figure 2 is a side view showing the shape inclined to install the storage unit of the present invention
  • Figure 3 according to an embodiment of the present invention 4 is a conceptual diagram showing the configuration of a bioreactor for in vitro proliferation of cells
  • FIG. 4 and FIG. 5 are conceptual views showing that pressure is released and released from the storage unit
  • FIG. It is a perspective view which shows the holder part with an addition.
  • the bioreactor for in vitro proliferation of cells includes a stirring part 100, a base part 200, a holder part 300, a storage part 400 and It includes an adjusting unit 500.
  • the present invention is supported by the stirring portion 100, the base portion 200 coupled to the upper portion of the stirring portion, the holder portion 300 is installed vertically from one surface of the base portion, the base is supported by the holder, It is mounted on one side of the unit, and includes a storage unit 400 for storing cells to be proliferated, such as hematopoietic stem cells collected from the human body and a control unit 500 connected to the storage unit for applying and releasing pressure into the storage unit.
  • a storage unit 400 for storing cells to be proliferated, such as hematopoietic stem cells collected from the human body
  • a control unit 500 connected to the storage unit for applying and releasing pressure into the storage unit.
  • FIGS. 1 to 2 a bioreactor for in vitro proliferation of cells according to an embodiment of the present invention will be described in more detail with reference to FIGS. 1 to 2.
  • the stirring unit 100 is to create a uniform mixed state of two or more substances having different physical or chemical properties by using external mechanical energy, mixing while applying pressure to the hematopoietic stem cells stored in the storage unit 400 to be described later Device.
  • the stirring unit 100 may include a housing and may include a motor and a gear inside the housing (not shown).
  • the housing may be a driving unit moving up, down, left, and right by the above-described motor and gear. It may be configured (not shown).
  • the cells to proliferate in the bioreactor for in vitro proliferation of cells is not limited to 'hematopoietic stem cells', including hematopoietic stem cells or hematopoietic stem cells extracted from a patient or a donor and non-adherent It is possible to proliferate non-adherent cells.
  • the base unit 200 may be coupled to one side of the stirring unit 100 to move in response to the movement of the stirring unit 100.
  • the base part 200 is located above the stirring part 100, as shown in FIG. 1, and is coupled to the driving part formed on the upper part of the stirring part 100. That is, the base unit 200 may be moved in response to the movement of the driving unit moving up, down, left, and right as the base unit 200 is connected to the motor and gears and coupled to the driving unit.
  • the base 200 includes a plate 210 and the protrusion 220.
  • the plate 210 is formed in a flat plate shape and may have a constant thickness.
  • Protruding portion 220 may be formed to protrude a plurality on one surface of the plate (210).
  • the protrusions 210 may include a plurality of protrusions protruding in a hemispherical shape from an upper surface of the plate 210 at regular intervals.
  • the protrusion 210 prevents the storage 400 from slipping from the base part by friction of the protrusion even when the storage part 400 is mounted on the plate 210 and the stirring part 100 is stirred.
  • the protrusion 220 may apply an appropriate pressure to the hematopoietic stem cells stored in the storage unit 400 during the stirring movement of the stirring unit 100.
  • an uneven portion having a plurality of grooves formed at regular intervals may be formed on the upper surface of the base portion 200 (not shown).
  • Holder portion 300 is installed vertically from one surface of the base portion 200 is connected to the upper portion of the storage portion 400 to support the storage portion. That is, as shown in FIG. 1, the holder part 300 serves to vertically mount the storage part 400 on the upper surface of the base part 200, and for this purpose, the holder part 300 has a pair of supports. 310 and the fastening part 320.
  • the pair of supports 310 are installed to be vertically spaced apart from each other from the upper surface of the plate 210. More specifically, the pair of supports 310 has a sliding rail formed therein so that the fastening part 320 is inserted into and coupled to the rail, so that vertical movement can be performed smoothly in the vertical direction along the rail.
  • the fastening part 320 is connected between the pair of supports 310 to support the storage part 400. More specifically, the fastening part 320 is a rod-shaped extending in the longitudinal direction, and is made of a tong structure that is separated from side to side so that the upper portion of the storage unit 400 is fitted, it is connected to both sides of the pair of supports 310
  • the storage unit 400 serves to stand vertically.
  • the pair of supports 310 moves the fastening part 320 in the up and down direction according to the amount of proliferating cells stored in the storage part 400, so that the angle and the height of the storage part mounted on the base part can be adjusted. As shown in FIG. 2, the pair of supports 310 adjusts the height of the fastening part 320 so that the storage part 400 coupled with the fastening part 320 has a desired angle on one surface of the base part 200. It is possible to mount inclined by.
  • the storage unit 400 stores cells such as hematopoietic stem cells to be proliferated and is supported by a support and mounted on one surface of the base unit 200.
  • the storage unit 400 may be a blood bag generally used to contain blood, and may be made of any one of a sealed pouch, a pack, and a bag.
  • FIG. 3 is a conceptual diagram showing the configuration of a bioreactor for in vitro proliferation of cells according to an embodiment of the present invention.
  • the adjusting unit 500 is connected to the storage 400 to control the application or release of pressure into the storage 400.
  • the adjusting unit 500 includes a pressure supply unit 510 and a pressure release unit 520.
  • the pressure supply part 510 is connected to the storage part 400 and applies pressure to the inside of the storage part 400.
  • the pressure supply unit 510 includes a first tube tube 511, a compressor 512, a filter unit 513, and a solenoid valve unit 514.
  • the first tube tube 511 is formed in a hollow shape, one side is inserted into the storage unit 400, and the other side is connected to the compressor 512 to apply pressure from the compressor to the storage unit 400. Play a role.
  • the compressor 512 is connected to the other side of the first tube tube to apply a pressure may be made of an air compressor structure.
  • the compressor 512 compresses ambient air to supply compressed air to the inside of the storage unit 400 through the first tube tube 511.
  • the filter unit 513 is disposed between the storage unit 400 and the compressor 512 to purify contaminants that may flow into the storage unit 400. More specifically, the filter unit 513 may be formed of a rectangular main body and a filter inserted into the main body (not shown).
  • the filter may include a pre-filter for filtering large dust, a deodorizing filter for removing odors in the air, and a candle. It is also possible to comprise a hepa filter for removing fine dust.
  • the main body is formed in a structure that is separated there is an advantage that the exchange of the filter is easy. As such, the filter unit 513 cleans compressed air generated from the compressor 512 and supplies clean air to the storage unit 400 to prevent contamination of hematopoietic stem cells.
  • the solenoid valve 514 is located between the storage unit 400 and the filter unit 513 serves to control the flow of pressure.
  • the pressure of the solenoid valve is preferably 18 Kpa (kilopascal) to 22 Kpa (kilopascal).
  • the 18 Kpa to 22 Kpa pressure is similar to the pressure blood receives in the bone marrow. Therefore, the solenoid valve 514 may maintain a constant pressure of 18Kpa to 22Kpa to maintain in vitro growth and performance of hematopoietic stem cells in the storage unit 400.
  • the pressure release unit 520 serves to release the pressure pressurized into the storage unit 400.
  • the pressure release part 520 includes a second tube tube 521 and a clamp 522.
  • the second tube tube 521 is connected to the first tube tube, the tube is separated from the first tube tube and may be formed in a hollow shape.
  • the second tube tube 521 is a tube separated from one side of the outer circumferential surface of the first tube tube connected to the storage unit 400, and is located between the storage unit 400 and the solenoid valve 514.
  • the clamp 522 is connected to the other side of the second tube tube 521 to apply or release pressure to the reservoir as the clamp is opened and closed.
  • the adjusting unit 500 including the pressure supply unit 510 and the pressure release unit 520 may further include a control unit 530.
  • the controller 530 may control the solenoid valve 514 and the clamp 522 to control supply of a constant pressure to the storage unit 400 and release of the supplied pressure.
  • the controller 530 controls to close the clamp 522 when the pressure is applied to the storage.
  • the controller 530 controls the solenoid valve 514 to close and the clamp 522 to open slowly when the pressure of the storage unit is back pressured.
  • FIG. 6 shows a bioreactor for in vitro proliferation of cells according to another embodiment of the present invention.
  • the bioreactor for in vitro proliferation of cells shown in FIG. 6 includes a holder part including an accommodating part and an inclined part in a base part.
  • the base part 200 ′ includes a plate 210 ′ having a flat plate shape and a partition wall 220 ′ extending vertically from a plate edge at a predetermined height.
  • the holder part 300 ' is mounted on the upper surface of the base part 200' and includes an accommodating part 310 'having a space for accommodating a storage part therein and an inclined part 320 inclined in one direction on a lower surface of the accommodating part. Contains').
  • the accommodating part 310 ′ is preferably opened at one side of the upper surface to accommodate the storage part 400.
  • the holder part 300 ' can be stably mounted in a state in which the storage part 400 is inclined to the base part 200', it is possible to maximize the stirring effect of the hematopoietic stem cells.

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
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Abstract

La présente invention concerne un bioréacteur pour la prolifération de cellules in vitro, et le bioréacteur pour la prolifération de cellules in vitro comprend : une partie d'agitation et une partie de base couplée à un côté de la partie d'agitation ; un support conçu pour être perpendiculaire à une surface de la partie de base ; une partie de stockage, qui stocke des cellules à faire proliférer, comprenant des cellules hématopoïétiques collectées à partir d'un corps humain, et qui est montée sur une surface de la partie de base ; et une partie de pression et une partie de commande pour appliquer ou relâcher une pression à l'intérieur de la partie de stockage.
PCT/KR2018/002943 2017-03-17 2018-03-13 Bioréacteur pour la prolifération de cellules in vitro Ceased WO2018169284A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0033771 2017-03-17
KR1020170033771A KR101895195B1 (ko) 2017-03-17 2017-03-17 세포 체외 증식을 위한 바이오리액터

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WO2018169284A1 true WO2018169284A1 (fr) 2018-09-20

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021087311A1 (fr) * 2019-10-30 2021-05-06 Brammer Bio Ma Llc Plate-forme d'agitation permettant de maintenir l'homogénéité de solutions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6190913B1 (en) * 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
CN101812405A (zh) * 2010-04-30 2010-08-25 惠倪 多边形塑料袋摇摆细胞反应器及培养动物细胞的方法
WO2011005773A2 (fr) * 2009-07-06 2011-01-13 Genentech, Inc. Procédé de culture de cellules eucaryotes

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US6190913B1 (en) * 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
WO2011005773A2 (fr) * 2009-07-06 2011-01-13 Genentech, Inc. Procédé de culture de cellules eucaryotes
CN101812405A (zh) * 2010-04-30 2010-08-25 惠倪 多边形塑料袋摇摆细胞反应器及培养动物细胞的方法

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Title
ANONYMOUS: "Biostat® RM", SARTORIUS STEDIM BIOTECH GMBH, 6 January 2012 (2012-01-06), XP055608773, Retrieved from the Internet <URL:http://microsite.sartorius.com/fileadmin/sartorius_pdf/alle/biotech/Data_BIOSTAT-RM_SBI2038-e.pdf> *
SINGH, V.: "Disposable bioreactor for cell culture using wave-induced agitation", CYTOTECHNOLOGY, 1999, pages 149 - 158, XP019236621, DOI: doi:10.1023/A:1008025016272 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021087311A1 (fr) * 2019-10-30 2021-05-06 Brammer Bio Ma Llc Plate-forme d'agitation permettant de maintenir l'homogénéité de solutions
CN115209980A (zh) * 2019-10-30 2022-10-18 布拉默生物Ma有限责任公司 用于保持溶液均质性的摇动平台
CN115209980B (zh) * 2019-10-30 2024-12-13 布拉默生物Ma有限责任公司 用于保持溶液均质性的摇动平台

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