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WO2018009729A3 - Modification d'adn polymérases pour des applications in vitro - Google Patents

Modification d'adn polymérases pour des applications in vitro Download PDF

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Publication number
WO2018009729A3
WO2018009729A3 PCT/US2017/040994 US2017040994W WO2018009729A3 WO 2018009729 A3 WO2018009729 A3 WO 2018009729A3 US 2017040994 W US2017040994 W US 2017040994W WO 2018009729 A3 WO2018009729 A3 WO 2018009729A3
Authority
WO
WIPO (PCT)
Prior art keywords
modification
vitro applications
dna polymerases
pcr
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2017/040994
Other languages
English (en)
Other versions
WO2018009729A2 (fr
Inventor
Mark Welch
Sridhar Govindarajan
David Mead
Baigen MEI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dna20 Inc dba Atum
Lucigen Corp
Original Assignee
Dna20 Inc dba Atum
Lucigen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna20 Inc dba Atum, Lucigen Corp filed Critical Dna20 Inc dba Atum
Publication of WO2018009729A2 publication Critical patent/WO2018009729A2/fr
Publication of WO2018009729A3 publication Critical patent/WO2018009729A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne des séquences d'ADN polymérase pour des applications in vitro comprenant la PCR pour la création de banques de séquençage de nouvelle génération, et pour la RT-PCR.
PCT/US2017/040994 2016-07-06 2017-07-06 Modification d'adn polymérases pour des applications in vitro Ceased WO2018009729A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662359059P 2016-07-06 2016-07-06
US62/359,059 2016-07-06
US201662360627P 2016-07-11 2016-07-11
US62/360,627 2016-07-11

Publications (2)

Publication Number Publication Date
WO2018009729A2 WO2018009729A2 (fr) 2018-01-11
WO2018009729A3 true WO2018009729A3 (fr) 2018-02-15

Family

ID=60913159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/040994 Ceased WO2018009729A2 (fr) 2016-07-06 2017-07-06 Modification d'adn polymérases pour des applications in vitro

Country Status (1)

Country Link
WO (1) WO2018009729A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4157856A2 (fr) * 2020-05-26 2023-04-05 Qiagen Beverly, LLC Enzyme polymérase
CN115948364B (zh) * 2022-10-24 2025-05-06 翌圣生物科技(上海)股份有限公司 Taq DNA聚合酶突变体Taq001及其编码基因、表达质粒、原核表达宿主

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8093030B2 (en) * 2005-10-06 2012-01-10 Lucigen Corporation Thermostable viral polymerases and methods of use
US20130022980A1 (en) * 2009-02-04 2013-01-24 Lucigen Corporation Rna- and dna-copying enzymes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8093030B2 (en) * 2005-10-06 2012-01-10 Lucigen Corporation Thermostable viral polymerases and methods of use
US20130022980A1 (en) * 2009-02-04 2013-01-24 Lucigen Corporation Rna- and dna-copying enzymes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHOI, JS ET AL.: "The Use of Modified and Non- Natural Nucleotides Provide Unique Insights into Pro-Mutagenic Replication Catalyzed by Polymerase eta", NUCLEIC ACIDS RESEARCH, vol. 44, 29 December 2015 (2015-12-29), pages 1022 - 1035, XP055462517 *
MEAD, D ET AL.: "Next Generation DNA Polymerases for Sequencing and Amplification", SIMPLIFYING GENOMICS, 5 January 2015 (2015-01-05) *

Also Published As

Publication number Publication date
WO2018009729A2 (fr) 2018-01-11

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