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WO2018009384A1 - Cuvette et système de rotor pour séparation de plasma sanguin - Google Patents

Cuvette et système de rotor pour séparation de plasma sanguin Download PDF

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Publication number
WO2018009384A1
WO2018009384A1 PCT/US2017/039647 US2017039647W WO2018009384A1 WO 2018009384 A1 WO2018009384 A1 WO 2018009384A1 US 2017039647 W US2017039647 W US 2017039647W WO 2018009384 A1 WO2018009384 A1 WO 2018009384A1
Authority
WO
WIPO (PCT)
Prior art keywords
cuvette
plasma
sample chamber
housing
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2017/039647
Other languages
English (en)
Inventor
James Kelland
Kevin J. Sullivan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Instruments LLC
Original Assignee
Advanced Instruments LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Instruments LLC filed Critical Advanced Instruments LLC
Publication of WO2018009384A1 publication Critical patent/WO2018009384A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents

Definitions

  • Centrifugation continues to be the most common method of obtaining plasma from whole blood. This method requires relative centrifugal forces (RCF) of 2000 to 5000 x g for 3 to 10 minutes (where "g” is acceleration due to gravity). The equipment necessary to generate these conditions is generally bulky and operates at high speeds. Re-mixing of blood cells with the plasma after separation can be a problem with this method, requiring either special techniques or special sample tubes to be employed. Therefore, for smaller instruments and so-called "point-of-care" devices that require clean plasma, centrifugation is not an ideal method.
  • a cuvette and rotor system for blood plasma separation is provided that addresses the above shortcomings.
  • the rotor system comprises a rotor assembly mounted for spinning about an axis.
  • the rotor assembly includes a cuvette holder to hold the cuvette at a fixed, non-zero angle with respect to the axis.
  • plasma that has been separated from red and white blood cells in the membrane is subjected to relative centrifugal forces, in some embodiments of about 20 to about 500 x g for about 1 minute, causing it to be drawn into the sample chamber.
  • the sample chamber can be sized for an anticipated sample volume, such that the centrifugal force simultaneously excludes any air from the chamber, thereby ensuring the chamber is full.
  • the plasma can then be optically tested within the sample chamber in the cuvette or can be withdrawn from the sample chamber for testing outside of the cuvette.
  • the rotor system can include an optical detection assembly for optical testing of the plasma in the cuvette while the cuvette remains in the rotor system after spinning.
  • the blood plasma separation system can be used with a range of blood sample sizes.
  • a greater volume of plasma can be obtained from a whole blood sample using a semipermeable medium than with prior art blood plasma separation devices that use a semipermeable medium.
  • the system provides fast separation and collection of plasma, and is a relatively gentle process that minimizes hemolysis and contamination. Because of the lower forces and speeds, the system can be much smaller and lighter than that needed for the conventional high speed centrifugation method.
  • a bubble-free plasma sample can be obtained. The sample can be tested in some embodiments directly in the rotor assembly.
  • Fig. 4 is a top plan view of the cuvette of Fig. 1;
  • Fig. 5 is a cross-sectional view along line A- A of Fig. 4;
  • Fig. 7 is an isometric view of an interior of the rotor system of Fig. 6;
  • Fig. 8 is a partially cutaway view of the rotor system of Fig. 7;
  • Fig. 9 is a cross-sectional view of an optical detector of the rotor system of Fig. 7;
  • Fig. 11 is a top plan view of the cuvette of Fig. 10;
  • Fig. 12 is a cross-sectional view along line A-A of Fig. 11;
  • Fig. 13 is a partially cutaway view of a plasma retrieval system of a rotor system
  • Fig. 14 is an isometric view of a bottom piece of a further embodiment of a cuvette with a reaction chamber
  • Fig. 15 is an isometric view of the cuvette bottom piece of Fig. 14 with sample in a reaction chamber;
  • Fig. 16 is an isometric view of the cuvette bottom piece of Fig. 14 with sample in a sample chamber;
  • Fig. 19 is an isometric top view of a further embodiment of a cuvette incorporating a scoop
  • Fig. 20 is an isometric bottom view of the cuvette of Fig. 19;
  • Fig. 21 is a plan view of the cuvette of Fig. 19;
  • Fig. 22 is a cross-sectional view along line A- A of Fig. 21;
  • Fig. 23 is an isometric exploded view of an embodiment of a cuvette with an associated cap
  • Fig. 24 is an isometric view of the cuvette of Fig. 23 with the cap in place;
  • Fig. 25 is an isometric exploded view of a further embodiment of a cuvette; and Fig. 26 is an exploded isometric view of a still further embodiment of a cuvette.
  • a blood plasma separation system having a cuvette 10 to collect and contain a blood sample and an accompanying rotor system 80 to hold and position the cuvette during a plasma separation process.
  • the sample chamber 30 includes aligned optical transmission windows 32, 34 for performing an optical test on a plasma sample in the sample chamber.
  • the top and bottom pieces 52, 62 of the cuvette housing can be formed from a transparent material that permits transmission of a light beam for performing an optical test on a plasma sample within the sample chamber.
  • plasma can be withdrawn from the sample chamber for further testing outside of the sample chamber, described further below.
  • an additional reaction chamber can be provided between the surface 64 and the sample chamber, also described further below.
  • the semipermeable membrane 40 for blood separation can be formed from a material such as asymmetric polysulfone arranged with pore sizes that decrease from the inlet side to the outlet side, or from a bound glass fiber material.
  • the larger red and white blood cells remain trapped within the larger pores of the membrane, while plasma is able to permeate through the depth of the membrane to the outlet side.
  • the membrane can include substantially uniform pores sized to exclude red and white blood cells while allowing plasma to permeate through.
  • Suitable semipermeable membranes for blood separation are commercially available as VividTM from Pall Corporation or VF2 from GE Healthcare.
  • the cuvette housing 20 includes a top piece 52 and a bottom piece 62 fastened together to hold the membrane in place.
  • the membrane access opening 22 is formed through the top piece.
  • the membrane 40 is attached to an underside 54 of the top piece 52, around the periphery 56 of and fully covering the access opening 22.
  • the bottom piece 62 includes the collection surface 64 that aligns with the membrane access opening 22.
  • the collection surface can have an area larger than the membrane access opening.
  • the outlet side 44 of the membrane 40 is in contact with the collection surface 64 of the bottom piece 62.
  • the motor 97 is supported on a base 99 and is connected to the rotor assembly 81 at the flat support plate 87 to cause the rotor assembly to spin.
  • the rotor assembly and motor are enclosed within a housing 102 for safety and to protect the rotor assembly from dirt and other contaminants.
  • the housing includes an upper surface 104 which can have an access opening 106 formed therein for inserting a cuvette.
  • a cuvette containing a sample to be separated and in some embodiments, analyzed can be placed through the opening into the cuvette holder 86 within the rotor assembly.
  • a lid 108 can be provided to close the opening once a cuvette has been inserted into the cuvette holder for the duration of the test.
  • a whole blood sample is deposited onto the inlet side of the membrane 40 of a cuvette 10.
  • the blood can either be collected onto the membrane directly or one of many available collection devices can be used.
  • the blood sample is absorbed into the pores of the membrane with the red blood cells being excluded toward the inlet side 42 and clean plasma concentrated toward the outlet side 44 as absorption takes place.
  • the cuvette 10 is placed into the rotor assembly 81 in the position shown in Figs. 7-9 with the sample chamber toward the bottom and the B side of the membrane facing upwardly and outwardly.
  • the rotor assembly 81 is spun about its central axis 96 to create centrifugal forces within the cuvette. The direction of the centrifugal forces and the orientation of the cuvette cause the separated plasma to flow from the collection surface 64 adjacent the membrane into the sample chamber 30.
  • the rotational speed and duration of the rotor assembly can be minimized to what is necessary to pull the plasma from the collection surface 64 into the sample chamber 30.
  • the rotation speed can be selected to provide a relative centrifugal force (RCF) that ranges from 20 to 500 x g (where "g" is acceleration due to gravity).
  • RCF relative centrifugal force
  • g acceleration due to gravity
  • the sample chamber volume can be designed to be slightly smaller than an anticipated plasma volume so that the chamber is completely full at the completion of the spin. Parameters such as the size of the rotor assembly, angle of cuvette, acceleration, speed of spin, and time of spin can be selected based on the specific application.
  • the rotor system can include one or more optical detection assemblies 110 to measure one or more components of interest in the plasma.
  • a detection assembly is shown in Figs. 7-9.
  • the optical detection assembly includes a light source 114 on one side of the rotor assembly 81 and a detector 116 on the other side of the rotor assembly.
  • the light source and the detector can be fixed and located in mounting blocks 123 and 124, respectively.
  • An optical axis 118 is defined from the light source to the detector, through the rotor assembly, and is perpendicular to the angle ⁇ at which the cuvette is held in the rotor assembly at the optical detection assembly.
  • the top and bottom pieces 83, 84 of the rotor assembly 81 can include apertures 98 aligned with the sample chamber 30 on the cuvette 10.
  • the rotor after spinning for a suitable time, the rotor can come to a stop at the optical detector within the rotor housing 102, with the apertures of the rotor and the sample chamber in the cuvette aligned on the optical axis 118.
  • the light source 114 can be activated to transmit a light beam through the sample chamber, through a filter(s) and/or lens(es) if present, to the detector 1 16.
  • the rotor assembly can be moved by the motor one way or the other to maximize the detected signal.
  • a home position for the cuvette holder at the optical detector can be specified, for example by an encoder on the motor or an optical sensor.
  • the optical measurement can be made while the rotor is spinning, as the cuvette passes the optical detector.
  • Fig. 18 illustrates an embodiment of a cuvette 510 in which the housing 520 is generally T-shaped, with a sloped surface 521 to collect a whole blood sample and direct the sample toward the semipermeable membrane in a rectangular collection window 522.
  • the top and bottom pieces of the cuvette housing can be formed of any suitable material. Plastics such as polycarbonate or metals such as aluminum can be used.
  • the cuvette can be transparent, translucent, or opaque. A transparent housing can be useful to determine visually if the sample chamber is sufficiently filled.
  • the semipermeable membrane can be attached around its perimeter to the underside of the top piece in order to create a fluid seal.
  • Various attachment methods can be used to attach the membrane, such as mechanical pressure, an adhesive, ultrasonic welding, or thermal welding.
  • the top and bottom pieces can then be bonded together to form the completed cuvette.
  • the top piece can include a peripheral lip that fits over the periphery of the bottom piece. Any suitable type of bonding can be used, such as an adhesive, ultrasonic welding, or thermal welding, or mechanical fastening. Other housing configurations can be used.
  • the cuvette and rotor system can be used for a variety of applications.
  • the cuvette and rotor system can be used to test for bilirubin, glucose, potassium, calcium, cholesterol, hemin, homocysteine, protein, uric acid, paracetamol (acetaminophen), creatinine, or triglycerides.
  • a diagnostic test for bilirubin a sample of blood of approximately 30 ⁇ _, is collected, for example, through a heel stick, onto or into the cuvette. Any excess blood is wiped from the cuvette, and the cuvette is capped (see Fig. 21) and inserted into the rotor.
  • the rotor spins the cuvette at a moderate relative centrifugal force (RCF), such as 50 g, for 30 to 60 sec, to pull plasma from the capillary surface into the sample chamber.
  • RCF moderate relative centrifugal force
  • An optical detector measures the amount of bilirubin.
  • the blood plasma separation system provides a number of advantages.
  • the system can be used with blood sample sizes ranging from several microliters to several milliliters.
  • a greater volume of usable plasma can be obtained from a whole blood sample using the system than with prior art blood plasma separation devices.
  • the system provides fast separation and collection of plasma, and is a relatively gentle process that minimizes hemolysis and contamination.
  • a bubble-free plasma sample can be obtained that is suitable for optical analysis or retrieval for other types of analysis.
  • a cuvette comprising:
  • a housing comprising an upper surface, an access opening in the upper surface
  • the membrane having an inlet side and an outlet side, the membrane having a pore size arranged to exclude red blood cells and white blood cells from the outlet side of the membrane and permit passage of plasma to the outlet side;
  • a collection surface adjacent the outlet side of the membrane within the housing; and a sample chamber within the housing in fluid communication with the collection surface to receive plasma.
  • the cuvette of embodiment 6, further comprising a first channel from the collection surface to the reaction chamber and a second channel from the reaction chamber to the sample chamber, wherein the second channel has a smaller cross-sectional area than the first channel.
  • a blood plasma separation system comprising:
  • a rotor system comprising a rotor assembly mounted for spinning about an axis, the rotor assembly comprising a cuvette holder to hold the cuvette at a fixed, non-zero angle with respect to the axis.
  • the rotor assembly comprises top and bottom truncated conical pieces nested together, and the cuvette holder comprises a cuvette recess between the top and bottom pieces.
  • any of embodiments 15-16 further comprising an optical detector assembly configured to optically test a plasma sample within the cuvette holder, the cuvette holder including apertures aligned with the sample chamber of the cuvette, the optical detector assembly comprising a light source and a light detector arranged on a light path, the light path passing through the apertures in the cuvette holder.
  • a plasma retrieval assembly including a syringe translatable toward and away from the sample chamber of the cuvette and operable to draw plasma in the sample chamber into the syringe.
  • a rotor system comprising:
  • a rotor assembly mounted for spinning about an axis, the rotor assembly comprising a cuvette holder to hold a cuvette at a fixed, non-zero angle with respect to the axis;
  • an optical detector assembly configured to optically test a plasma sample within a sample chamber of the cuvette, the optical detector assembly comprising a light source and a light detector arranged on a light path, the light path passing through the cuvette holder.
  • a rotor system comprising: a rotor assembly mounted for spinning about an axis, the rotor assembly comprising a cuvette holder to hold a cuvette at a fixed, non-zero angle with respect to the axis; and
  • a plasma retrieval assembly comprising a needle or cannula configured to withdraw plasma from the sample chamber of the cuvette.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Ecology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Measuring Cells (AREA)

Abstract

L'invention concerne un système de séparation de plasma sanguin, lequel système utilise une cuvette comprenant une membrane semi-perméable pour exclure les globules rouges et les globules blancs vis-à-vis d'un côté de sortie tout en permettant le passage du plasma. La cuvette comprend une surface de collecte adjacente au côté de sortie de la membrane et une chambre d'échantillon en communication vis-à-vis des fluides avec la surface de collecte pour recevoir le plasma. La cuvette peut être mise en rotation dans un système de rotor pour aspirer le plasma dans la chambre d'échantillon pour un test supplémentaire. Le système de rotor peut comprendre un ensemble de détection optique pour tester optiquement le plasma dans la chambre d'échantillon dans la cuvette quand elle est dans le système de rotor. Le système de rotor peut comprendre un ensemble de récupération de plasma pour retirer le plasma à partir de la chambre d'échantillon pour un test à l'extérieur de la cuvette.
PCT/US2017/039647 2016-07-06 2017-06-28 Cuvette et système de rotor pour séparation de plasma sanguin Ceased WO2018009384A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662358666P 2016-07-06 2016-07-06
US62/358,666 2016-07-06

Publications (1)

Publication Number Publication Date
WO2018009384A1 true WO2018009384A1 (fr) 2018-01-11

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PCT/US2017/039647 Ceased WO2018009384A1 (fr) 2016-07-06 2017-06-28 Cuvette et système de rotor pour séparation de plasma sanguin

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3983127A4 (fr) * 2019-06-12 2022-07-20 Siemens Healthcare Diagnostics, Inc. Dispositif de séparation de plasma et de mesure d'échantillon, kits et procédés d'utilisation associés

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3720502A (en) * 1970-12-21 1973-03-13 Beckman Instruments Inc Centrifuge test tube stopper
US5681529A (en) * 1994-08-25 1997-10-28 Nihon Medi-Physics Co., Ltd. Biological fluid analyzing device
US6153148A (en) * 1998-06-15 2000-11-28 Becton, Dickinson And Company Centrifugal hematology disposable
US20150185233A1 (en) * 2012-08-08 2015-07-02 KONINKLIJKE PHILIPS N.V. a corporation Method and apparatus for separating plasma from blood for bilirubin level estimation
US20150204788A1 (en) * 2012-07-25 2015-07-23 Theranos, Inc. Image analysis and measurement of biological samples
WO2015193194A1 (fr) * 2014-06-16 2015-12-23 Roche Diagnostics Gmbh Cartouche dotée d'un couvercle rotatif
WO2016025726A1 (fr) * 2014-08-13 2016-02-18 Vivebio, Llc Réseau de membranes analytiques, et dispositif de séparation de plasma l'incorporant
US20160091517A1 (en) * 2014-09-29 2016-03-31 C A Casyso Ag Blood Testing System and Method

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3720502A (en) * 1970-12-21 1973-03-13 Beckman Instruments Inc Centrifuge test tube stopper
US5681529A (en) * 1994-08-25 1997-10-28 Nihon Medi-Physics Co., Ltd. Biological fluid analyzing device
US6153148A (en) * 1998-06-15 2000-11-28 Becton, Dickinson And Company Centrifugal hematology disposable
US20150204788A1 (en) * 2012-07-25 2015-07-23 Theranos, Inc. Image analysis and measurement of biological samples
US20150185233A1 (en) * 2012-08-08 2015-07-02 KONINKLIJKE PHILIPS N.V. a corporation Method and apparatus for separating plasma from blood for bilirubin level estimation
WO2015193194A1 (fr) * 2014-06-16 2015-12-23 Roche Diagnostics Gmbh Cartouche dotée d'un couvercle rotatif
WO2016025726A1 (fr) * 2014-08-13 2016-02-18 Vivebio, Llc Réseau de membranes analytiques, et dispositif de séparation de plasma l'incorporant
US20160091517A1 (en) * 2014-09-29 2016-03-31 C A Casyso Ag Blood Testing System and Method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3983127A4 (fr) * 2019-06-12 2022-07-20 Siemens Healthcare Diagnostics, Inc. Dispositif de séparation de plasma et de mesure d'échantillon, kits et procédés d'utilisation associés
US11850583B2 (en) 2019-06-12 2023-12-26 Siemens Healthcare Diagnostics Inc. Plasma separation and sample metering device and kits and methods of use related thereto

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