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WO2018004252A1 - Composition pour activer la croissance des poils ou inhiber la chute des poils utilisant un extrait de feuille de rosmarinus officinalis (romarin) - Google Patents

Composition pour activer la croissance des poils ou inhiber la chute des poils utilisant un extrait de feuille de rosmarinus officinalis (romarin) Download PDF

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Publication number
WO2018004252A1
WO2018004252A1 PCT/KR2017/006833 KR2017006833W WO2018004252A1 WO 2018004252 A1 WO2018004252 A1 WO 2018004252A1 KR 2017006833 W KR2017006833 W KR 2017006833W WO 2018004252 A1 WO2018004252 A1 WO 2018004252A1
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Prior art keywords
hair
composition
extract
essential oil
rosemary
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Ceased
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PCT/KR2017/006833
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English (en)
Korean (ko)
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최한준
박상용
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Individual
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/20—Natural extracts
    • A23V2250/21—Plant extracts

Definitions

  • the present invention is rosemary ( Rosmarinus officinalis ) relates to a composition for promoting hair growth or inhibiting hair loss using a leaf extract.
  • Hair is formed from hair follicles, with about 5 million adult hair follicles distributed throughout the body, except for palms and seagrass, with 1 million heads, of which 100,000 are present in the scalp. (Bulletin of Food Technology 2009, 22 (4): 651-663). Scalp hair accounts for about 20% of human hair, and has the function of protecting the head from shocks by buffering the head along with the physiological functions of the hair such as sweat evaporation and temperature control. It also has important social functions for personality and image production (Clin Dermatol 2001, 19 (2): 161-166).
  • hair follicle which is a very complex organ consisting of internal hair root, external hair root, hair shaft, and matrix cells, and hair dermal cells among various types of hair follicles.
  • papilla cells play an important role in hair growth.
  • Dermal papilla cells a type of dermal fibroblast specialized in hair bulbs, are not only normal hair cycles but also hair growth through the growth of dermal papilla cells in symptoms such as androgen alopecia (male hair loss). It plays an important role in controlling growth. Thus, factors affecting the proliferation of dermal papilla cells in hair loss are important targets for improving hair loss.
  • alopecia refers to an increase in the number of hairs that fall abnormally due to a shorter growth rate and a longer degenerative or resting period during these cycles (Differentiation, 2004, 72 (9-10): 489; Dev Cell. , 2001, 1 (1): 13; J. Invest.Dermatol., 2005, 125 (1): 42).
  • dihydrotestosterone is another testosterone produced by the testosterone called testosterone by type 2 5 ⁇ -reductase.
  • Minoxidil preparations see US Pat. Nos. 4,139,619 and 4,596,812) and finasteride (US patents). 5555457, 5760046 or Korean Patent No. 375083).
  • Minoxidil preparations are known to reduce hair loss by up to 10% in men with alopecia, but have the disadvantage of not having a very good effect on hair loss outside the kiln and can cause depression. There is a problem in that they have side effects such as decreased libido, erectile dysfunction. For this reason, efforts have recently been made to find substances having a hair growth promoting or hair loss inhibiting activity in natural products with few side effects.
  • the present invention discloses hair growth promoting activity and the like of the rosemary extract.
  • the mixture of essential oil extract of rosemary leaf by carnosic acid and hexane extraction method is rosema by carnosic acid or hexane extraction method. It was confirmed that the activity of the essential oil extract of the leaves of Lee, and especially the activity of 1: 2 mixture of essential oil extract of rosemary leaves by carnosic acid and hexane extraction method. Furthermore, the inventors of the present invention showed that when the animal experiment was conducted using a mouse depilated using a mixture of 1: 2 of the essential oil extract of rosemary leaf by carnosic acid and hexane extraction method, which was the most active in the cell test results, It was confirmed that the hair density and thickness were increased along with the promotion.
  • Carnosic acid (CAS # 3650-09-7) is a benzenediol abietane diterpene found in rosemary ( Rosmarinus officinalis ), Salvia ( Salvia officinalis ), and has a strong antioxidant activity. , Anti-cancer activity by inducing apoptosis in breast cancer cells, ovarian cancer cells, prostate cancer cells and the like (International Journal of Molecular Sciences. 2014; 15 (7) 12698-12713).
  • composition for promoting hair growth inhibition or hair loss of the present invention comprises (i) a rosemary leaf extract or (ii) a mixture of carnosic acid and rosemary leaf essential oil as an active ingredient. It is done.
  • active ingredient alone refers to a component that can exhibit the desired activity or exhibit itself with a carrier which is inactive.
  • hair loss should be understood as including all the symptoms classified as hair loss in the art regardless of the direct and indirect cause of the occurrence. Specifically, it includes all of the symptoms of hair loss due to poor blood circulation, excessive sebum secretion due to excessive production of dihydrotestosterone, deterioration of the scalp function by peroxides or bacteria, aging, genetic factors, stress, and their complex actions. .
  • extract refers to water, methanol, ethanol, butanol lower alcohol having 1 to 4 carbon atoms, acetone, methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, Supercritical extraction solvents such as extracts obtained by leaching with N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, carbon dioxide, pentane, etc.
  • DMF N-dimethylformamide
  • DMSO dimethylsulfoxide
  • the extraction method is applied to any method, such as cold needle, reflux, heating, ultrasonic radiation, supercritical extraction in consideration of the polarity, degree of extraction, the degree of preservation of the active substance can do.
  • the fractionated extract includes a fraction obtained by suspending the crude extract in a specific solvent and mixing and standing with a solvent having a different polarity.
  • the crude extract is sequentially fractionated using the solvents in increasing or decreasing polarity.
  • the meaning of the extract includes a concentrated liquid or solid extract from which the extraction solvent is removed by lyophilization, vacuum drying, hot air drying, spray drying, or the like.
  • it preferably means an extract obtained by leaching using water, acetone or a mixed solvent thereof as an extraction solvent (in particular, an extract obtained using a mixed solvent of water and acetone).
  • rosemary leaf essential oil extract means the extract of the oil phase obtained by condensing the volatile component of the rosemary leaf to be extracted.
  • methods known in the art such as steam distillation, solvent extraction using volatile solvents (benzene, hexane, etc.), supercritical extraction, can be used.
  • rosemary leaf essential oil extract refers to an extract obtained by using hexane as a volatile solvent, specifically, an extract obtained by dehydration after reflux cooling with hexane.
  • the effective amount is 0.0001% by weight to 15% by weight based on the total weight of the composition. Will be determined within the% range.
  • the term "effective amount" herein refers to the present invention, which may exhibit an intended hair growth promoting effect or hair loss inhibiting effect when the composition of the present invention is administered to a mammal, preferably a human, during the administration period according to the suggestion of a medical professional or the like. Refers to the amount of the active ingredient included in the composition of. Such effective amounts can be determined experimentally within the range of ordinary skill in the art.
  • the food composition of the present invention may be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, ionic beverages, processed oils such as milk, yogurt, gums, rice cakes, sweets, bread, sweets, noodles, and the like. It can be prepared as a dietary supplement, such as foods, tablets, capsules, pills, granules, liquid, powder, flaky, paste, syrup, gel, jelly, bar.
  • the food composition of the present invention can distinguish any product as long as it conforms to the enforcement regulations at the time of manufacture and distribution in the legal and functional divisions.
  • it may be a health functional food according to the Act on Health Functional Food, or confectionary, bean, tea, beverage, special purpose food, etc. according to each food type in the Food Code of the Food Sanitation Act (KFDA notification, standards and standards of food). .
  • the food composition of the present invention may include food additives in addition to the active ingredient.
  • Food additives can generally be understood as substances that are added to, mixed with, or infiltrated in the manufacture, processing, or preservation of foods, and because they are taken daily with food and for a long time, their safety must be ensured.
  • the Food Additives Code of the Food Sanitation Act (KFDA Notification, Food Additives Standards and Standards) defines the food additives that are guaranteed to be safe by dividing them into chemical synthetics, natural additives, and mixed preparations. In sweeteners, flavors, preservatives, emulsifiers, acidulants, thickeners and the like can be classified.
  • Sweeteners are used to impart a proper sweetness to foods, and may be natural or synthesized.
  • a natural sweetener is used.
  • sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.
  • Flavoring agents can be used to enhance the taste or aroma, both natural and synthetic. It is the case of using a natural thing preferably.
  • the natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like.
  • ginseng red ginseng
  • bamboo shoots aloe vera, ginkgo and the like
  • Natural flavors can be liquid concentrates or solid extracts.
  • a synthetic flavor may be used, and as the synthetic flavor, esters, alcohols, aldehydes, terpenes, and the like may be used.
  • calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid) and the like can be used, and as an emulsifier, acacia gum, carboxymethylcellulose, xanthan gum, pectin Etc. may be used, and acidic acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like may be used.
  • the acidulant may be added so that the food composition is at an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing the taste.
  • suspending implementers As the thickener, suspending implementers, sedimenting agents, gel formers, swelling agents and the like can be used.
  • the food composition of the present invention may include a bioactive substance or minerals known in the art for the purpose of supplementing and reinforcing the functionality and nutritional properties and ensuring the stability as a food additive.
  • physiologically active substances include catechins, vitamin B1, vitamin C, vitamin E, vitamin B12 and the like, tocopherol, dibenzoylthiamine, and the like contained in green tea.
  • minerals include calcium preparations such as calcium citrate and magnesium stearate.
  • Magnesium preparations, iron preparations such as iron citrate, chromium chloride, potassium iodine, selenium, germanium, vanadium, zinc and the like.
  • the food additive as described above may be included in an amount that can achieve the purpose of addition according to the product type.
  • composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.
  • the pharmaceutical composition of the present invention can be classified into any product as long as it conforms to the relevant enforcement regulations at the time of manufacture and distribution in the legal and functional divisions.
  • it may be a quasi-drug or drug under the Pharmaceutical Affairs Act or, if it is a drug, it may be a generic or specialized drug.
  • compositions of the present invention may be prepared in oral or parenteral formulations according to the route of administration by conventional methods known in the art, including pharmaceutically acceptable carriers in addition to the active ingredients.
  • pharmaceutically acceptable here means that the subject of application (prescription) is not toxic as far as adaptable without inhibiting the activity of the active ingredient.
  • composition of the present invention is prepared in an oral dosage form, powders, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, suspensions, wafers according to methods known in the art with suitable carriers It may be prepared in a formulation such as.
  • suitable pharmaceutically acceptable carriers include lactose, glucose, sucrose, dextrose, sugars such as sorbitol, mannitol, xylitol, starch such as corn starch, potato starch, wheat starch, cellulose, methylcellulose, ethylcellulose, Celluloses such as sodium carboxymethylcellulose, hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable Yu etc. can be mentioned. If formulated, it may be formulated to include diluents and / or excipients, such as fillers, extenders, binders, wetting agents, disintegrants, surfactants, if necessary.
  • diluents and / or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants, if necessary.
  • compositions of the present invention When the pharmaceutical compositions of the present invention are prepared in parenteral formulations, they may be formulated in the form of injections, transdermal administrations, nasal inhalants and suppositories with suitable carriers according to methods known in the art.
  • suitable carriers may be sterile water, ethanol, polyols such as glycerol or propylene glycol, or mixtures thereof, preferably PBS (phosphate buffered saline) containing Ringer's solution, triethanol amine or sterilized for injection. Water, isotonic solutions such as 5% dextrose, and the like.
  • transdermal administration When formulated as a transdermal administration, it may be formulated in the form of an ointment, cream, lotion, gel, external solution, pasta, linen, aerosol, and the like.
  • Nasal inhalants can be formulated in the form of aerosol sprays using suitable propellants, such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc. witepsol), tween 61, polyethylene glycols, cacao butter, laurin paper, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearate, sorbitan fatty acid esters and the like.
  • suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc. witepsol
  • tween 61 polyethylene glycols, cacao butter, laurin paper, polyoxyethylene
  • Preferred dosages of the pharmaceutical compositions of the present invention range from 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g, depending on the condition, body weight, sex, age, severity of the patient and route of administration. It can range from / kg. Administration can be done once a day or divided into several times. Such dosage should not be construed as limiting the scope of the invention in any aspect.
  • composition of the present invention can be understood as a hair cosmetic composition for external application to hair or scalp.
  • the hair cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, shampoo, soap, surfactant-containing cleansing, oil, powder foundation
  • Product forms include emulsion foundations, wax foundations, and sprays.
  • it may be a softening lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder formulation.
  • the cosmetic composition of the present invention may include, in addition to the active ingredient, components conventionally used in the cosmetic composition, for example, conventional adjuvants such as stabilizers, solubilizers, surfactants, vitamins, pigments and pharmaceuticals, and carriers.
  • conventional adjuvants such as stabilizers, solubilizers, surfactants, vitamins, pigments and pharmaceuticals, and carriers.
  • the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
  • animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
  • animal oils vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide
  • cellulose derivatives polyethylene glycols
  • silicones bentonites
  • silicas talc or zinc oxide
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.
  • a solvent, solubilizing agent or emulsifying agent is used as the carrier component, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan and the like can be used.
  • a liquid diluent such as water, ethanol or propylene glycol
  • a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, and microcrystals
  • carrier components such as water, ethanol or propylene glycol
  • ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, and microcrystals are used as carrier components.
  • Castle cellulose, aluminum metahydroxy, bentonite, agar and the like can be used.
  • the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide.
  • Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
  • the hair cosmetic composition of the present invention can be prepared according to the manufacturing method of the cosmetic composition usually carried out in the art, except that the active ingredient is included.
  • according to the present invention can provide a composition for promoting hair growth or inhibiting hair loss using the rosemary extract.
  • composition of the present invention can be commercialized into food, drugs, hair cosmetics and the like.
  • Figure 2 is a result of measuring the cell growth promoting effect when treated with rosemary leaf extract and the like in human keratinocytes (HaCaT cells).
  • Figure 3 is a result of measuring the hair growth promoting effect when the rosemary leaf extract and the like treated to C57BL / 6 mice.
  • Figure 4 is the result of measuring the hair density and thickness when the rosemary leaf extract and the like treated with C57BL / 6 mice.
  • Figure 5 is the result of observing histological changes when treated with rosemary leaf extract, etc. in C57BL / 6 mice.
  • Figure 6 is the result of measuring the expression level of Bcl-2 and Bax when treated with rosemary leaf extract, etc. in C57BL / 6 mice.
  • Samples were (i) essential oil extract of rosemary leaf by hexane extraction (A), (ii) essential oil extract of rosemary leaf by steam distillation (B), (iii) carnoic acid (C), (iv) carnoic acid and hexane Mixture of 1: 5 (carnoic acid: essential oil, weight ratio) of essential oil extract of rosemary leaf by extraction method (D), (v) 1: 2 (carnoic acid: essential oil) of essential oil extract of rosemary leaf by carnosic acid and hexane extraction method , A total of five were used as a sample (E).
  • the essential oil extract of rosemary leaf by hexane extraction was added 5 times weight of hexane to the rosemary leaf powder, extracted by reflux at 70-80 °C for 2 hours, washed with sodium hydroxide, and after obtaining a nucleic acid layer, the hexane layer was dried with anhydrous sodium sulfate. Dehydrated and concentrated under reduced pressure,
  • the essential oil extract of the rosemary leaf by steam distillation is put into the distillation apparatus using rosemary leaf, and steamed at 100 ° C for 1 hour to extract the essential oil.
  • the extracted essential oil is recovered in a cooling tube and dehydrated with anhydrous sodium sulfate. It was prepared by.
  • Carnosic acid was purchased from Sigma (St. Louis, MO, USA) and used.
  • the results of three replicates are shown in FIG. 1 as a percentage compared to the negative control group, which is the sample untreated group.
  • the essential oil extract (A) by hexane extraction method is better than the essential oil extract (B) by steam distillation method of human dermal papilla cells proliferation effect, a mixture of essential oil extract by carnosic acid and hexane extraction method (D and It can be seen that E) is significantly superior to carnoic acid (C) or its essential oil extract (A). In particular, the mixture (E) of 1: 2 was the best.
  • FIG. 2 shows that the essential oil extract (A) by the hexane extraction method is superior to the essential oil extract (B) by the steam distillation method, and the growth effect of human dermal papilla cells is superior to that of the experiment example 1, and the carnosic acid and hexane extraction method.
  • Mixtures of essential oil extracts (D and E), especially mixtures (E) of 1: 2 show the best.
  • mice The back hairs of the 7-week-old C57BL / 6 mice were removed, and five mice with clear back skin were divided into 4 groups. Hair on the back of the mouse was removed by destroying hair follicles using a Wax-Rosin mixture.
  • the first group was applied with 70% ethanol for 13 days (negative control)
  • the second group was applied with Hyundai drug minoxidil for 13 days (positive control)
  • the third group was Experimental Examples 1 and 2 1: 2 mixture of carnosic acid and essential oil extract by hexane extraction (E, R3) was dissolved in DMSO and diluted to 5% (v / v) in 70% ethanol. Was applied.
  • FIG. 3 shows the results of measuring the growth and distribution of hair at 3 days, 7 days, 9 days, 11 days and 13 days after depilation.
  • the results of measuring the density and thickness of the hair by performing close-up photography of the hair on the 3rd and 11th day after depilation are shown in FIG. 4.
  • mice were killed on the 13th day after hair removal, and tissues of the hairy areas of the dorsal skin were collected. Fixed with% formaldehyde solution. After fixation, washed with water, dehydrated, embedded in paraffin, 7 ⁇ m sections were prepared, and stained with Hematoxylin & Eosin (H & E). The results of the histological changes are shown in FIG. 5.
  • mice of each group were lethal on the 13th day after hair removal, and the tissues of the hair growth areas of the dorsal skin were collected and put into liquid nitrogen. After freezing, the protein was separated from the frozen tissue, and the expression levels of Bcl-2 and Bax were confirmed by Western Blot method.

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Abstract

L'invention concerne une composition pour activer la croissance des poils ou inhiber la chute des poils utilisant un extrait de feuille de Rosmarinus Officinalis (romarin) présentant des activités d'augmentation de la densité et de l'épaisseur des poils en activant la prolifération des cellules de bulbe pileux et des kératinocytes humains et en activant la croissance des poils lorsqu'elle sert à traiter une souris épilée.
PCT/KR2017/006833 2016-06-28 2017-06-28 Composition pour activer la croissance des poils ou inhiber la chute des poils utilisant un extrait de feuille de rosmarinus officinalis (romarin) Ceased WO2018004252A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20160080815 2016-06-28
KR10-2016-0080815 2016-06-28
KR1020160164623A KR101724510B1 (ko) 2016-06-28 2016-12-05 로즈마리 잎 추출물을 이용한 발모 촉진 또는 탈모 억제용 조성물
KR10-2016-0164623 2016-12-05

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WO2018004252A1 true WO2018004252A1 (fr) 2018-01-04

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112107527A (zh) * 2020-09-22 2020-12-22 广东嘉丹婷日用品有限公司 一种防脱精华液及其制备方法
CN112190510A (zh) * 2020-09-22 2021-01-08 广东嘉丹婷日用品有限公司 一种防脱发组合物及其制备方法
CN112274463A (zh) * 2020-09-22 2021-01-29 广东嘉丹婷日用品有限公司 一种防脱洗发露及其制备方法
CN115814045A (zh) * 2022-11-03 2023-03-21 山东新创生物科技有限公司 一种用于改善脱发的外泌体复合制剂及其制备方法与应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101724510B1 (ko) * 2016-06-28 2017-04-07 최한준 로즈마리 잎 추출물을 이용한 발모 촉진 또는 탈모 억제용 조성물
KR102097302B1 (ko) * 2018-04-30 2020-04-06 주식회사 하이솔 식물 유래 오일을 유효성분으로 함유하는 모발 성장촉진 또는 탈모방지용 조성물
KR101910595B1 (ko) 2018-05-15 2018-10-22 (주)대덕바이오 터티오펜 유도체를 함유하는 탈모치료 및 발모촉진용 조성물

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08291026A (ja) * 1995-04-17 1996-11-05 Hayashibara Biochem Lab Inc 発育毛剤
KR20000076549A (ko) * 1999-01-29 2000-12-26 레블론 컨수머 프로덕츠 코오포레이션 헤어 로숀
KR20130052243A (ko) * 2011-11-11 2013-05-22 (주)아모레퍼시픽 카르노스산 또는 그 유도체를 포함하는 피부 자극 완화제
KR20160015187A (ko) * 2015-08-19 2016-02-12 (주)웰어스 모발 건강용 농축 음료 조성물, 그 제조 방법 및 그를 포함하는 모발 건강용 천연차
KR101724510B1 (ko) * 2016-06-28 2017-04-07 최한준 로즈마리 잎 추출물을 이용한 발모 촉진 또는 탈모 억제용 조성물

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08291026A (ja) * 1995-04-17 1996-11-05 Hayashibara Biochem Lab Inc 発育毛剤
KR20000076549A (ko) * 1999-01-29 2000-12-26 레블론 컨수머 프로덕츠 코오포레이션 헤어 로숀
KR20130052243A (ko) * 2011-11-11 2013-05-22 (주)아모레퍼시픽 카르노스산 또는 그 유도체를 포함하는 피부 자극 완화제
KR20160015187A (ko) * 2015-08-19 2016-02-12 (주)웰어스 모발 건강용 농축 음료 조성물, 그 제조 방법 및 그를 포함하는 모발 건강용 천연차
KR101724510B1 (ko) * 2016-06-28 2017-04-07 최한준 로즈마리 잎 추출물을 이용한 발모 촉진 또는 탈모 억제용 조성물

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112107527A (zh) * 2020-09-22 2020-12-22 广东嘉丹婷日用品有限公司 一种防脱精华液及其制备方法
CN112190510A (zh) * 2020-09-22 2021-01-08 广东嘉丹婷日用品有限公司 一种防脱发组合物及其制备方法
CN112274463A (zh) * 2020-09-22 2021-01-29 广东嘉丹婷日用品有限公司 一种防脱洗发露及其制备方法
CN115814045A (zh) * 2022-11-03 2023-03-21 山东新创生物科技有限公司 一种用于改善脱发的外泌体复合制剂及其制备方法与应用

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