[go: up one dir, main page]

WO2018092678A1 - Procédé de traitement thermique pour micro-organisme - Google Patents

Procédé de traitement thermique pour micro-organisme Download PDF

Info

Publication number
WO2018092678A1
WO2018092678A1 PCT/JP2017/040430 JP2017040430W WO2018092678A1 WO 2018092678 A1 WO2018092678 A1 WO 2018092678A1 JP 2017040430 W JP2017040430 W JP 2017040430W WO 2018092678 A1 WO2018092678 A1 WO 2018092678A1
Authority
WO
WIPO (PCT)
Prior art keywords
temperature
microorganism
bifidobacteria
concentrate
heat treatment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2017/040430
Other languages
English (en)
Japanese (ja)
Inventor
和典 柏木
麻美 土屋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meiji Co Ltd
Original Assignee
Meiji Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meiji Co Ltd filed Critical Meiji Co Ltd
Priority to CN201780070859.1A priority Critical patent/CN109923205A/zh
Publication of WO2018092678A1 publication Critical patent/WO2018092678A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Definitions

  • the present invention relates to a method for heat treatment of microorganisms, and more particularly to a method for heat treatment of microorganisms that can be used for foods and drinks, pharmaceuticals, and the like.
  • Probiotic foods are foods containing microorganisms such as bacteria that are considered to have a positive effect on the human body when taken into the human body.
  • microorganisms such as bacteria that are considered to have a positive effect on the human body when taken into the human body.
  • yogurt containing live lactic acid bacteria can be mentioned as a probiotic food.
  • dead microorganisms of microorganisms that can be used in probiotic foods contain active ingredients that have a positive effect on the human body depending on the type of microorganisms
  • microorganisms contained in probiotic foods are dead cells. There may be.
  • the microorganisms contained in the probiotic food are dead cells, the microorganism does not act in the probiotic food, so that the quality of the probiotic food can be stably maintained. is there.
  • Japanese Patent Application Laid-Open No. 2008-24569 discloses Lactobacillus paracasei KW311 strain, which is a kind of lactic acid bacteria, as a bacterium that can be used in probiotic foods.
  • Japanese Patent Application Laid-Open No. 2008-245569 discloses that food and drink having an antiallergic function can be produced by blending dead bacteria of Lactobacillus paracasei KW311 strain that has been heat-sterilized into food and drink.
  • the dead cells of microorganisms used in probiotic foods can be obtained, for example, by heat sterilization of living microorganisms (live bacteria).
  • live bacteria living microorganisms
  • live cells and the like aggregate in a liquid containing live bacteria to form aggregates.
  • the aggregate formed by heat sterilization is added to the probiotic food as it is, the consumer may feel rough due to the aggregate and the texture of the probiotic food may be reduced.
  • the temperature at the time of heat sterilization may be set to, for example, 100 ° C. or lower. By lowering the temperature at the time of heat sterilization, it is possible to suppress the progress of denaturation of the protein forming the microorganism, which is the cause of the formation of aggregates.
  • the hot water heating is a method of heating an object to be heated, for example, by circulating hot water heated to a predetermined temperature (100 ° C. or less) around the object to be heated.
  • heat sterilization includes steam heating using steam at 100 ° C. or higher, but from the viewpoint of preventing the progress of aggregation, a method using steam heating is not appropriate.
  • the hot water heating has a problem that it takes time to heat the object to be heated to a predetermined temperature, and the efficiency of the heat sterilization is poor. Furthermore, when heat-sterilizing a liquid containing viable bacteria using hot water heating, the substantial heating time of the liquid containing viable bacteria may be prolonged, and formation of aggregates including dead cells may proceed. .
  • an object of the present invention is to provide a microorganism heat treatment method that can prevent the formation of aggregates.
  • the method for heat treatment of microorganisms according to the present invention includes a) and b) steps.
  • a liquid containing microorganisms is heated to a predetermined sterilization temperature using vacuum steam.
  • the temperature of the liquid containing the microorganisms heated in the step a) is maintained at a sterilization temperature for a predetermined time using vacuum steam.
  • the method for heat treating a microorganism according to the present invention may further include a step c).
  • the liquid containing the microorganisms cultured in the step c) is heated.
  • the step c) may include a step c-1) and a step c-2).
  • step c-1 the microorganism is inoculated into the medium, and the microorganism inoculated in the medium is cultured.
  • step c-2 a concentrated solution of microorganisms is produced from the medium containing the microorganisms cultured in step c-1).
  • a process heats a concentrate.
  • the step a) may include the steps a-1) and a-2).
  • the step a-1) when the temperature of the liquid containing microorganisms is a predetermined intermediate temperature lower than the sterilization temperature, the liquid containing microorganisms is heated using vacuum vapor having a temperature not lower than the intermediate temperature and not higher than the sterilization temperature.
  • the step a-2) when the temperature of the liquid containing microorganisms is not lower than the intermediate temperature and not higher than the sterilization temperature, the liquid containing microorganisms is heated using vacuum vapor having a temperature not lower than the sterilization temperature.
  • the intermediate temperature may be an aggregate formation temperature at which aggregates are generated in a liquid containing microorganisms.
  • the temperature of the vacuum vapor may be adjusted by changing the pressure of the vacuum vapor.
  • the step a) uses a jacket tank including a tank portion into which a liquid containing microorganisms is charged and a jacket portion having an internal space to which vacuum vapor is supplied.
  • the internal space may be depressurized to a pressure lower than the atmospheric pressure.
  • aggregates can be prevented from being formed when heat treatment is performed on microorganisms.
  • FIG. 1 It is a flowchart which shows the heat processing method of the microorganisms which concern on embodiment of this invention. It is the schematic of the structure of the heat processing apparatus used at the heating process shown in FIG. It is a graph which shows the time change of the temperature in the concentrate of the bifidobacterium which concerns on Example 2 of this invention. It is a graph which shows the time change of the temperature in the concentrate of the bifidobacterium which concerns on the comparative example 2 of this invention.
  • a microorganism is cultured to produce a culture solution, the produced culture solution is concentrated to produce a concentrate, and the microorganisms contained in the concentrate using vacuum vapor It is a method of heating.
  • microorganisms contained in the concentrate are sterilized by heating the microorganism concentrate using the microorganism heat treatment method according to the present embodiment, it is possible to add the heat-treated concentrate to food and drink it can.
  • the microorganism is a microorganism that can be used for probiotic foods and pharmaceuticals.
  • the microorganism to be heat-treated is a microorganism that is considered to have a good influence on the human body by being taken into the human body.
  • the microorganisms that can use the microorganism heat treatment method according to the present embodiment are bacteria such as lactic acid bacteria and bifidobacteria.
  • lactic acid bacteria include Lactobacillus, Streptococcus, and Lactococcus, which are mainly used for the production of fermented milk.
  • examples of lactic acid bacteria include meat products and genus Leuconostoc and Pediococcus used for producing dairy products other than fermented milk.
  • Bifidobacteria are bacteria belonging to the genus Bifidobacterium.
  • Vacuum vacuum is a type of water vapor. Specifically, vacuum vapor refers to water vapor having a temperature lower than 100 ° C. and a pressure lower than atmospheric pressure.
  • FIG. 1 is a flowchart showing a microorganism heat treatment method according to the present embodiment.
  • the heat treatment method for microorganisms according to the present embodiment includes a culture process (step S1), a concentration process (step S2), and a heat treatment process (step S3).
  • FIG. 1 the steps shown in FIG. 1 will be described in detail, taking as an example the case where the microorganism is a bifidobacteria.
  • step S1 First, in the culturing step (step S1), bifidobacteria are cultured.
  • the medium used for the culture of bifidobacteria only needs to contain a nutrient source by which bifidobacteria can grow.
  • the medium only needs to contain at least one of milk-derived components, yeast extract, soybean extract, peptone, sugars, and minerals.
  • milk-derived components include whey, casein, skim milk powder, whey protein concentrate (WPC), and whey protein isolate (WPI).
  • Peptone is, for example, a tripty case.
  • the saccharide include monosaccharides such as glucose and disaccharides such as lactose.
  • the mineral for example, whey mineral can be used.
  • the medium is sterilized, for example, at 121 ° C. or higher for 1 to 10 minutes. Then, the bifidobacteria are inoculated to the sterilized medium and neutralized culture of the bifidobacteria is performed.
  • the temperature of the medium and the pH of the medium are not particularly limited as long as they are suitable for the growth of the bifidobacteria.
  • the concentration of bifidobacteria is preferably 10 7 to 10 11 cfu / mL.
  • a medium suitable for the growth of the microorganism to be cultured may be selected.
  • the temperature and pH during the culture may be maintained under conditions suitable for the growth of the microorganism to be cultured.
  • step S2 After the culturing step (step S1), the bifidobacteria culture solution is concentrated to produce a bifidobacteria concentrate (step S2).
  • Centrifugation is used to concentrate the culture solution of Bifidobacterium. Centrifugation is performed, for example, after cooling the bifidobacteria culture solution to a temperature of 10 ° C. and then applying a centrifugal force of 5,000 to 20,000 ⁇ G to the bifidobacteria culture solution for a residence time of about 2 to 10 minutes. Do as.
  • the centrifuge used in the concentration step (step S2) is preferably a continuous type in order to efficiently centrifuge a large amount of bifidobacteria culture solution.
  • the continuous centrifugal separator is supplied with liquid (supplying bifidobacteria culture solution), draining (obtaining bifidobacteria culture solution in which bifidobacteria are concentrated), and discharging (bifidobacteria). It is possible to carry out in parallel with the discharge of the supernatant of the culture broth of the fungus.
  • this Embodiment does not prevent use of centrifuges other than a continuous type.
  • the bifidobacteria culture solution is separated into a lower-layer bifidobacteria culture solution containing bifidobacteria at a high concentration and an upper layer light solution (supernatant) containing almost no lactic acid bacteria.
  • the recovery rate of bifidobacteria in the concentration step (step S2) is preferably 95% or more.
  • the concentration of bifidobacteria in the concentrated solution of bifidobacteria produced in the concentration step (step S2) is, for example, 10 8 to 10 12 cfu / mL.
  • centrifugation when centrifuging the culture solution of microorganisms other than Bifidobacterium.
  • the centrifugal force and residence time applied to the culture solution of microorganisms other than bifidobacteria may be appropriately changed so that microorganisms other than bifidobacteria can be concentrated at a recovery rate of 95% or more.
  • step S3 As shown in FIG. 1, the concentrated solution of bifidobacteria produced by the concentration step (step S2) is heat-treated using vacuum steam (step S3).
  • the heating step (step S3) is a step of heating the Bifidobacterium concentrate to a predetermined target temperature and maintaining the Bifidobacterium concentrate heated to the predetermined target temperature for a predetermined time.
  • the target temperature is, for example, a sterilization temperature that can sterilize bifidobacteria contained in the concentrate.
  • the bifidobacteria contained in the concentrated liquid are sterilized, and a concentrated liquid of dead cells of the bifidobacteria is generated.
  • a concentrated solution of Bifidobacterium dead cells is used as a raw material for probiotic foods and pharmaceuticals.
  • FIG. 2 is a schematic diagram of the configuration of the heat treatment apparatus 100 used in the heating step (step S3) shown in FIG.
  • the heat treatment apparatus 100 includes a vacuum vapor generator 1, a jacket tank 2, pipes 3 to 5, and a control device 6.
  • the vacuum steam generator 1 is a device that generates vacuum steam.
  • the jacket tank 2 is a container for heat-treating the bifidobacteria concentrate.
  • the pipe 3 connects the vacuum steam generator 1 and the internal space 22 ⁇ / b> A of the jacket portion 22 in the jacket tank 2.
  • One end of the pipe 4 is connected to the bottom of the internal space 22 ⁇ / b> A of the jacket portion 22.
  • the other end of the pipe 4 is a discharge port for discharging the condensed water in the internal space 22A.
  • One end of the pipe 5 is connected to a joint (not shown) provided in the pipe 4, and the other end of the pipe 5 is connected to the vacuum steam generator 1.
  • the pipe 5 is used for the vacuum steam generator to collect the vacuum steam flowing into the pipe 4. Based on the temperature of the bifidobacteria concentrate measured by the temperature sensor 24 and the pressure of the vacuum steam measured by the pressure sensor 25, the control device 6 controls the vacuum steam from the vacuum steam generator 1 to the internal space 22 ⁇ / b> A. Control the amount of supply.
  • the temperature sensor 24 and the pressure sensor 25 will be described later.
  • the jacket tank 2 includes a tank part 21, a jacket part 22, a stirring device 23, a temperature sensor 24, and a pressure sensor 25.
  • the tank unit 21 is a cylindrical container into which a concentrated solution of bifidobacteria is introduced.
  • the jacket portion 22 is a hollow container that covers the outer peripheral side surface and the bottom surface of the tank portion 21.
  • a hollow portion in the jacket portion 22 is an internal space 22A.
  • the vacuum steam supplied from the vacuum steam generator 1 is condensed into water.
  • the tank portion 21 and the jacket portion 22 are formed of a metal having high thermal conductivity and are thermally connected.
  • the stirring device 23 is a stirring blade for stirring the concentrated solution of bifidobacteria put into the tank unit 21.
  • the temperature sensor 24 measures the temperature of the bifidobacteria concentrated solution introduced into the tank unit 21.
  • the pressure sensor 25 is disposed in the internal space 22A and measures the pressure of the vacuum vapor supplied to the internal space 22A.
  • the heating process includes a charging process (step S31), a pressure reducing process (step S32), a temperature increasing process (step S33), and a temperature maintaining process (step S34).
  • the heating step (step S3) will be described in detail with reference to FIG. 1 and FIG. 2 by taking as an example the case where the concentrated solution of bifidobacteria is maintained at 80 ° C. for 10 minutes.
  • step S31 ⁇ Injection process (step S31) and decompression process (step S32) ⁇
  • step S31 the concentrated solution of bifidobacteria produced in step S2 is charged into the tank unit 21 (step S31).
  • step S32 the internal space 22A of the jacket portion 22 is decompressed to a pressure lower than the atmospheric pressure (step S32).
  • the internal space 22A If the internal space 22A is not depressurized, the influence of the atmosphere (nitrogen and oxygen) on the atmospheric pressure and temperature in the internal space 22A increases, and it becomes difficult to adjust the temperature of the vacuum vapor in the internal space 22A.
  • the internal space 22A By reducing the internal space 22A in advance, it becomes easy to adjust the temperature of the vacuum vapor in the internal space 22A to a desired temperature.
  • step S33 After reducing the internal space 22A of the jacket portion 22 in step S32, vacuum vapor is supplied to the internal space 22A to heat the Bifidobacteria concentrate introduced into the tank portion 21 (step S33).
  • step S33 the temperature of the bifidobacteria concentrate is raised to the target temperature (80 ° C.).
  • step S33 the concentrated solution of bifidobacteria is stirred by rotating the stirring blade of the stirring device 23. By stirring the bifidobacteria concentrate, the bifidobacteria concentrate introduced into the tank unit 21 can be heated substantially uniformly.
  • the temperature of the vacuum vapor in the internal space 22A of the jacket part 22 is adjusted according to the temperature of the bifidobacteria concentrate put in the tank part 21. Specifically, when the temperature of the concentrated bifidobacteria charged in the tank unit 21 is equal to or lower than the intermediate temperature lower than the target temperature, vacuum vapor having a temperature equal to or higher than the intermediate temperature and equal to or lower than the target temperature. Use to heat the Bifidobacterium concentrate. Then, when the temperature of the bifidobacteria concentrate becomes equal to or higher than the intermediate temperature, the bifidobacteria concentrate is heated using vacuum steam that is equal to or higher than the target temperature.
  • the concentrated solution of bifidobacteria is rapidly heated by heating using the vacuum steam at a temperature equal to or higher than the intermediate temperature and lower than the target temperature.
  • the intermediate temperature is preferably an aggregate production temperature (for example, 60 ° C.) at which aggregates are produced in the bifidobacteria concentrate.
  • an aggregate production temperature for example, 60 ° C.
  • the intermediate temperature is preferably an aggregate production temperature (for example, 60 ° C.) at which aggregates are produced in the bifidobacteria concentrate.
  • the target temperature is set to 80 ° C. and the intermediate temperature is set to 60 ° C. at the start of heating the Bifidobacterium concentrate.
  • the temperature of the bifidobacteria concentrate is not higher than the intermediate temperature (60 ° C.), so the temperature of the vacuum vapor in the internal space 22A of the jacket portion 22 is 60 ° C. or higher and 65 ° C. or lower. Adjusted to When the temperature of the bifidobacteria concentrated liquid becomes an intermediate temperature (60 ° C.) or higher, the temperature of the vacuum vapor in the internal space 22A is adjusted to 80 ° C. or higher and 85 ° C. or lower.
  • the setting of the target temperature (80 ° C.) and the intermediate temperature (60 ° C.) in the heat treatment of the Bifidobacterium concentrate is an example, and a temperature different from the above temperature may be set as the target temperature and the intermediate temperature.
  • the internal space 22A of the jacket portion 22 is controlled to a pressure lower than the atmospheric pressure by a decompression device (not shown) or the vacuum steam generator 1. Since the condensation temperature of the vacuum vapor is uniformly determined by the atmospheric pressure, when the vacuum vapor is introduced into the space in which the pressure is controlled, the temperature of the vacuum vapor becomes the condensation temperature at that pressure.
  • the temperature of the vacuum vapor in the internal space 22A can be adjusted.
  • Control of the atmospheric pressure in the internal space 22A is performed by adjusting the flow rate of the vacuum vapor supplied to the internal space 22A.
  • the concentrated bifidobacteria charged in the tank portion 21 is heated by the latent heat released when the vacuum vapor condenses into liquid water in the internal space 22A of the jacket portion 22.
  • the temperature of the concentrated solution of bifidobacteria put into the tank unit 21 is, for example, 4 ° C. at the start of heating (immediately after centrifugation). Since the tank portion 21 and the jacket portion 22 are in thermal contact with each other, the vacuum vapor is cooled in the internal space 22 ⁇ / b> A of the jacket portion 22 by the concentrated bifidobacteria charged in the tank portion 21. As the vacuum cools, it releases latent heat and condenses into liquid water. The latent heat released by the condensation of the vacuum vapor is transmitted to the tank unit 21 and heats the bifidobacteria concentrate.
  • the liquid water generated by the condensation of the vacuum vapor moves to the bottom of the internal space 22A of the jacket portion 22 and is discharged from the pipe 5.
  • the condensation of the vacuum vapor acts in the direction of lowering the atmospheric pressure of the internal space 22A and lowering the temperature of the internal space 22A.
  • the atmospheric pressure in the internal space 22A is controlled by adjusting the flow rate of the vacuum steam. For this reason, since the supply of the vacuum vapor to the internal space 22A is continued, the heating of the bifidobacteria concentrate is continued by the latent heat released from the vacuum vapor.
  • the bifidobacteria concentrate is heated by the latent heat released from the vacuum vapor, but the vapor temperature is lower than 100 ° C. For this reason, the Bifidobacterium concentrate is not heated at a temperature of 100 ° C. or higher.
  • there is warm water heating as a method of heating the Bifidobacterium concentrate to a temperature of 100 ° C. or lower.
  • the latent heat released from the vacuum vapor to heat the Bifidobacterium concentrate
  • the Bifidobacterium concentrate can be heated in a shorter time than when heated by warm water heating. The reason will be described.
  • the latent heat is, for example, thermal energy released or absorbed when a substance changes from one phase (for example, gas) to another phase (for example, liquid).
  • a substance changes from one phase (for example, gas) to another phase (for example, liquid).
  • latent heat is released from the vacuum vapor.
  • the latent heat released from the vacuum vapor varies depending on the pressure of the vacuum vapor, but is larger than the sensible heat of liquid water and is approximately five times or more the sensible heat of liquid water.
  • the sensible heat of liquid water is the heat released when the temperature of the liquid water decreases or the heat absorbed when the temperature of the liquid water increases.
  • Hot water heating is a heating method that uses sensible heat of liquid water, and specifically, a method of heating a concentrated solution of bifidobacteria by filling the internal space 22A of the jacket portion 22 with hot water of a predetermined temperature. is there.
  • the latent heat of vacuum vapor is very large compared to the sensible heat of hot water. Therefore, the method of heating using the latent heat released from the vacuum vapor can heat the Bifidobacterium concentrate quickly in a short time compared to the method of heating using sensible heat (hot water heating). Become.
  • warm water heating requires more time to reach the target temperature than heating by vacuum steam.
  • the time during which the temperature of the bifidobacteria concentrate is near the aggregate formation temperature is increased, the generation of aggregates proceeds. That is, when warm water heating is used, huge aggregates are likely to be generated in the bifidobacteria concentrate.
  • it is necessary to increase the load of stirring That is, when warm water heating is used, it is necessary to set the tip speed of the stirring blade to a speed at which the aggregate can be crushed.
  • step S34 ⁇ Temperature maintenance process (step S34) ⁇
  • the bifidobacteria concentrate put in the tank unit 21 rises to the target temperature by the temperature rise process (step S33)
  • the bifidobacteria concentrate is kept at the target temperature for a predetermined time while the stirring blade continues to rotate. Maintain (step S34).
  • the pressure of the vacuum vapor in the internal space 22A of the jacket portion 22 is controlled so that the Bifidobacterium concentrate maintains the target temperature of 80 ° C. for 10 minutes.
  • vacuum steam is also used in the temperature maintaining step (step S34). As a result, it becomes easier to maintain the temperature of the bifidobacteria concentrate at 80 ° C. than when warm water heating is used.
  • the target temperature is set to 80 ° C. as an example.
  • warm water heating when the temperature of the Bifidobacterium concentrate heated to the target temperature (80 ° C.) exceeds 80 ° C., warm water having a temperature of 80 ° C. or less is supplied to the jacket portion 22 to warm water in the internal space 22A. It is necessary to lower the temperature. However, since the temperature of the hot water in the internal space 22A changes due to the convection of the hot water, it takes time until the temperature of the hot water reaches 80 ° C. or less in the entire internal space 22A.
  • the temperature of the vacuum steam in the internal space 22A is controlled by controlling the pressure of the vacuum steam in the internal space 22A. Since the vacuum steam is a gas, the change in the pressure of the vacuum steam in the internal space 22A is much faster than the change in the temperature of the hot water when the internal space 22A is filled with warm water. The temperature of the vacuum vapor in the internal space 22A can be adjusted frequently according to the temperature of the concentrated solution of bifidobacteria. Therefore, when the vacuum steam is used, it becomes easier to maintain the temperature of the Bifidobacterium concentrate at the target temperature (80 ° C.) than when warm water heating is used.
  • the heating step (step S3) is completed by maintaining the temperature of the Bifidobacterium concentrate at the target temperature for a predetermined time.
  • the Bifidobacterium concentrate after the heating step (step S3) is used as a raw material for probiotic foods and pharmaceuticals.
  • the heating temperature of the concentrated liquid of microorganisms other than bifidobacteria and the time to maintain the concentrated liquid at the heating temperature are the concentrated liquid. It changes suitably according to the kind of microorganisms contained. For example, when the concentrated liquid of microorganisms is sterilized by the heating process (step S3), the conditions under which the microorganisms are sterilized by heating differ depending on the microorganism.
  • the microorganism heat treatment method heats the microorganism concentrate using vacuum steam, and uses the vacuum steam to set the temperature of the microorganism concentrate at a predetermined temperature for a certain period of time. maintain.
  • the vacuum vapor supplied to the internal space 22A of the jacket portion 22 is at a temperature lower than 100 ° C.
  • the microbial concentrate is not heated to 100 ° C. or higher in the heating step (step S3). Therefore, since the progress of denaturation of the protein forming the microorganism and the protein derived from the medium due to heat can be suppressed, the progress of the formation of aggregates in the concentrated liquid of the microorganism can be suppressed.
  • the microbial concentrate can be heated to the target temperature more quickly than when using hot water heating. Therefore, since the substantial heating time of the concentrate of bifidobacteria can be shortened, formation of aggregates can be further prevented.
  • the present invention is not limited to this.
  • the culture solution of the microorganism may be heated as it is using vacuum steam without being centrifuged. That is, the liquid containing the cultured microorganisms may be heated using vacuum vapor. Or you may heat the liquid which disperse
  • the temperature of the vacuum vapor is adjusted in two stages, that is, a temperature not lower than the intermediate temperature and not higher than the target temperature and a temperature not lower than the target temperature in the temperature raising step (step S33).
  • the temperature of the vacuum steam may be adjusted in three or more stages, or the temperature of the vacuum steam may be adjusted to the target temperature from the beginning.
  • microorganisms lactic acid bacteria, bifidobacteria, etc.
  • You may heat-process the liquid containing microorganisms, such as other bacteria, by a heating process (step S3).
  • step S2 the example of using the centrifugal separation method to generate the bifidobacteria concentrated solution from the cultured solution of bifidobacteria has been described, but the present invention is not limited to this.
  • a method other than the centrifugation method for example, a membrane separation method or the like may be used.
  • the target temperature (sterilization temperature) is appropriately changed according to the type of microorganism contained in the concentrate.
  • the intermediate temperature when the intermediate temperature is set to the aggregate production temperature, the intermediate temperature may be appropriately changed based on the type of microorganisms contained in the concentrate, the components contained in the concentrate, and the like.
  • a concentrated solution containing only bifidobacteria is prepared in the culturing step (step S1) and the concentrating step (step S2), and the target temperature of the concentrated solution is sterilized in the heating step (step S3).
  • the concentrate may contain two or more types of microorganisms.
  • the target temperature of the concentrated solution may be a temperature at which at least one type of microorganisms among the microorganisms contained in the concentrated solution is sterilized. Further, the target temperature may be a temperature at which at least one kind of microorganisms among the microorganisms contained in the concentrate is inactivated.
  • a bifidobacteria culture solution was obtained by neutralizing the Bifidobacterium OLB6378 strain, which is a type of bifidobacteria, using a casein decomposition medium.
  • the casein decomposition medium is a medium using enzyme-degraded casein as a protein source.
  • the Bifidobacterium OLB6378 strain is a Bifidobacterium bifidum OLB6378 strain, and has been deposited with the patent microorganism deposition center of the National Institute of Technology and Evaluation under the accession number “NITE BP-31”.
  • the cell concentrate fraction obtained by centrifugation corresponds to the Bifidobacteria concentrate before heat treatment according to Example 1.
  • the cell concentration of bifidobacteria was 2 ⁇ 10 11 cfu / mL.
  • the cell concentration of bifidobacteria was measured using the method described in “Food Sanitation Inspection Guidelines for Microorganisms (1990)” issued by the Japan Food Sanitation Association.
  • Example 3 The Bifidobacterium concentrate before heat treatment according to Example 1 was put into a 100 L tank manufactured by Osaka Sanitary Co., Ltd. The temperature of the bifidobacteria concentrate according to Example 1 was 6.2 ° C. before the start of heating.
  • Comparative Example 1 As Comparative Example 1, the concentrated solution of bifidobacteria was heated using normal steam at 100 ° C. or higher.
  • Bifidobacterium bifidum OLB6378 strain which is a kind of bifidobacteria used in Example 1, was cultured by the culture method described in Example 1 above, and the Bifidobacterium according to Comparative Example 1 was cultured. A culture broth was obtained.
  • the concentrated cell fraction (43 kg) according to Comparative Example 1 was obtained from the Bifidobacterium culture according to Comparative Example 1.
  • the bacterial cell concentrated liquid fraction according to Comparative Example 1 corresponds to the Bifidobacteria concentrated liquid before heat treatment according to Comparative Example 1.
  • the bacterial cell concentration was 2 ⁇ 10 11 cfu / mL.
  • the method for measuring the cell concentration in Comparative Example 1 is the same as the method for measuring the cell concentration in Example 1.
  • the concentrate of Bifidobacterium before heat treatment according to Comparative Example 1 was put into a 50 L tank manufactured by Iwai Kogyo Co., Ltd.
  • the temperature of the Bifidobacterium concentrate according to Comparative Example 1 was 6.2 ° C. before the start of heating.
  • the Bifidobacterium concentrate according to Comparative Example 1 was heated to 80 ° C., and then the Bifidobacterium concentrate according to Comparative Example 1 was held at 80 ° C. for 10 minutes. Thereby, the concentrate of the bifidobacteria after the heat processing which concerns on the comparative example 1 was obtained.
  • the method for measuring aggregates will be described using the bifidobacteria concentrate after the heat treatment according to Example 1 as an example.
  • a dispersion was obtained by dispersing the concentrated solution of bifidobacteria after heat treatment according to Example 1 in water.
  • SALD-2000 laser diffraction particle size distribution analyzer
  • the average diameter, median diameter, and mode diameter of the aggregates contained in the dispersion were measured.
  • Table 1 shows a concentrated solution of bifidobacteria before heat treatment according to Example 1, a concentrated solution of bifidobacteria after heat treatment according to Example 1, and a concentrated solution of bifidobacteria after heat treatment according to Comparative Example 1.
  • the agglomerate size in is shown.
  • the bifidobacteria concentrate before heat treatment according to Example 1 is the smallest in any of the average diameter, median diameter, and mode diameter.
  • the average diameter, median diameter, and mode diameter are 1 to 1.7 ⁇ m, and these values correspond to the size of bifidobacteria. . That is, it turns out that the aggregate is not formed in the concentrate of the bifidobacteria before heat processing which concerns on Example 1.
  • the average diameter, the median diameter, and the mode diameter of the aggregates contained in the concentrated solution of bifidobacteria after the heat treatment according to Example 1 are included in the concentrated solution of bifidobacteria before the heat treatment according to example 1. It is larger than the average diameter, median diameter, and mode diameter of the aggregate. That is, it can be seen that by performing the heat treatment on the bifidobacteria concentrate according to Example 1, the formation of aggregates proceeds to some extent.
  • the aggregate contained in the Bifidobacterium concentrate after the heat treatment according to Example 1 is smaller than the aggregate contained in the Bifidobacterium concentrate after the heat treatment according to Comparative Example 1. That is, by performing heat treatment using vacuum steam on the concentrated solution of Bifidobacteria according to Example 1, the progress of the formation of aggregates is suppressed as compared with the case where heat treatment is performed at a temperature of 100 ° C. or higher. It became clear that we could do it.
  • the tip speed of the rotor blade in Example 1 is about 2/3 the size of the rotor blade in Comparative Example 1, but the aggregates contained in the concentrated bifidobacteria after heat treatment according to Example 1 are: It is smaller than the aggregate contained in the concentrated solution of bifidobacteria after the heat treatment according to Comparative Example 1. That is, it has been clarified that when heat treatment using vacuum vapor is performed, the formation of huge aggregates can be suppressed without performing stirring for pulverizing the aggregates.
  • Example 2 In the same procedure as in Example 1 above, 50 kg of a bifidobacteria concentrate according to Example 2 was obtained. 50 kg of the bifidobacteria concentrate according to Example 2 was placed in a 100 L tank manufactured by Osaka Sanitary Co., Ltd., and heated by supplying vacuum steam to the jacket portion of the 100 L tank. During the heating, 50 kg of the bifidobacteria concentrate according to Example 2 was stirred by rotating the stirring blade. The stirring conditions were that the rotation speed of the stirring blade was 120 rpm and the tip speed of the stirring blade was 0.88 m / sec. While adjusting the temperature of the vacuum vapor in the jacket according to the temperature rise of the bifidobacteria concentrate, the time change of the temperature of the bifidobacteria concentrate 50 kg was measured.
  • FIG. 3 is a graph showing the time change of the temperature of the concentrated solution of bifidobacteria in this example.
  • the set value of the temperature of the vacuum steam supplied to the jacket portion of the 100 L tank is changed in accordance with the temperature rise of the bifidobacteria concentrate.
  • the time until the temperature of the 50 kg of the Bifidobacterium concentrate reached 80 ° C. was 80 minutes.
  • FIG. 4 is a graph showing the time change of the temperature of the concentrated solution of bifidobacteria in this comparative example.
  • the temperature of the hot water supplied to the jacket portion of the 250 L tank is changed in accordance with the temperature rise of the bifidobacteria concentrate.
  • the time until the temperature of the 100 kg of the bifidobacteria concentrate reached 80 ° C. was 180 minutes.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

La présente invention aborde le problème consistant à fournir un procédé de traitement thermique pour un micro-organisme grâce auquel une formation d'agrégats peut être empêchée. Un micro-organisme est inoculé dans un milieu et y est cultivé (étape S1). Après la culture, le milieu est centrifugé pour acquérir ainsi un concentré liquide du micro-organisme (étape S2). Le concentré liquide du micro-organisme est chauffé à l'aide de vapeur sous vide (étape S3). À l'étape S3, le concentré liquide du micro-organisme est versé dans la partie réservoir d'un réservoir chemisé (étape S31), puis l'espace interne de la partie chemise du réservoir chemisé est dépressurisée (étape S32). Après dépressurisation de l'espace interne, de la vapeur sous vide est introduite dans l'espace interne de telle sorte que la température du concentré liquide du micro-organisme est élevée à une température de stérilisation (étape S33). Ensuite, le concentré liquide du micro-organisme est maintenu à la température de stérilisation pendant une période de temps prédéfinie à l'aide de vapeur sous vide (étape S34).
PCT/JP2017/040430 2016-11-16 2017-11-09 Procédé de traitement thermique pour micro-organisme Ceased WO2018092678A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201780070859.1A CN109923205A (zh) 2016-11-16 2017-11-09 微生物的加热处理方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016-223409 2016-11-16
JP2016223409A JP6915226B2 (ja) 2016-11-16 2016-11-16 微生物の加熱処理方法

Publications (1)

Publication Number Publication Date
WO2018092678A1 true WO2018092678A1 (fr) 2018-05-24

Family

ID=62145152

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2017/040430 Ceased WO2018092678A1 (fr) 2016-11-16 2017-11-09 Procédé de traitement thermique pour micro-organisme

Country Status (4)

Country Link
JP (1) JP6915226B2 (fr)
CN (1) CN109923205A (fr)
TW (1) TW201825005A (fr)
WO (1) WO2018092678A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7763029B2 (ja) * 2020-03-30 2025-10-31 森永乳業株式会社 発酵組成物
US20240277009A1 (en) * 2021-07-28 2024-08-22 Kirin Holdings Kabushiki Kaisha Lactic acid bacterium-containing beverage and method for producing same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04369302A (ja) * 1991-06-14 1992-12-22 Tlv Co Ltd 真空蒸気発生装置
JP2004041099A (ja) * 2002-07-12 2004-02-12 Combi Corp 免疫賦活効果を有する乳酸菌の製造法及び免疫賦活剤
JP2008005811A (ja) * 2006-06-30 2008-01-17 Snow Brand Milk Prod Co Ltd 乳酸菌の増殖促進剤および生残性向上剤
WO2009066537A1 (fr) * 2007-11-19 2009-05-28 Meiji Dairies Corporation Inducteur de fonction immunorégulatrice et composition alimentaire

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04369302A (ja) * 1991-06-14 1992-12-22 Tlv Co Ltd 真空蒸気発生装置
JP2004041099A (ja) * 2002-07-12 2004-02-12 Combi Corp 免疫賦活効果を有する乳酸菌の製造法及び免疫賦活剤
JP2008005811A (ja) * 2006-06-30 2008-01-17 Snow Brand Milk Prod Co Ltd 乳酸菌の増殖促進剤および生残性向上剤
WO2009066537A1 (fr) * 2007-11-19 2009-05-28 Meiji Dairies Corporation Inducteur de fonction immunorégulatrice et composition alimentaire

Also Published As

Publication number Publication date
TW201825005A (zh) 2018-07-16
JP2018078832A (ja) 2018-05-24
JP6915226B2 (ja) 2021-08-04
CN109923205A (zh) 2019-06-21

Similar Documents

Publication Publication Date Title
Farnsworth et al. Effects of transglutaminase treatment on functional properties and probiotic culture survivability of goat milk yogurt
JP6633552B2 (ja) 後酸性化の制御が改善された発酵乳製品の製造方法
CN102265925B (zh) 一种香蕉益生菌凝固型酸奶及其制备方法
JP4862053B2 (ja) 新規乳酸菌
JP6535285B2 (ja) シトルリンの調製方法
CN103547162B (zh) 物理性质改善的发酵乳的制备方法
CN108102987B (zh) 一种空间罗伊氏乳杆菌ss23-52及其干粉发酵剂的制备与在纯种益生菌酸奶中的应用
WO2010139765A2 (fr) Procédé de fabrication d'un produit laitier fermenté
JP4802216B2 (ja) ビフィドバクテリウム属菌含有組成物及びビフィドバクテリウム属菌含有組成物の製造方法
JP2016189709A (ja) 発酵乳の製造方法
JP6915226B2 (ja) 微生物の加熱処理方法
CN102586137B (zh) 一种产胆盐水解酶乳酸菌干粉发酵剂的制备方法
US20230104298A1 (en) Fermented Soy Beverage Concentrate and Method for Producing the Same
Wang et al. Comparison of the physicochemical property and volatile flavour compounds of yoghurt made from ultra-pasteurised and membrane-filtered milk
CN108124958A (zh) 一种高蛋白发酵乳及其生产方法
CN103813715A (zh) 液态发酵乳及其制造方法
CN108531426A (zh) 提高益生菌热耐受性的方法
JP4794592B2 (ja) 新規乳酸菌
JP2019041662A (ja) 大豆粉を使用した乳酸菌発酵物の製造方法
CN103876151A (zh) 一种益生菌微生态胶囊剂及其制备方法
JP2005270066A (ja) 豆乳及びその加工食品
CN108902982A (zh) 一种益生菌微胶囊冻干粉及其制备方法
CN117651495A (zh) 含有乳酸菌的饮料及其制造方法
US20200359640A1 (en) Methods for producing fermented milk and lactic acid bacteria starter
CN107841518A (zh) 一种南极磷虾抗氧化肽及其制备方法与应用

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17871520

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 17871520

Country of ref document: EP

Kind code of ref document: A1