WO2018062790A1 - Novel microorganism chryseobacterium camelliae dolsongi-ht1 with keratinolytic activity and use thereof - Google Patents

Abstract

Disclosed in the present specification is a novel microorganism Chryseobacterium camelliae Dolsongi-HT1 with keratinolytic activity. The novel microorganism was isolated from green tea leaves and has outstanding keratinolytic activity at 37°C even in the condition of weak acid, such as pH 6. In addition, the present specification provides a cosmetic composition comprising the novel microorganism, a culture thereof, or keratinase isolated therefrom as an effective ingredient for keratin removal.

Description

Nodules-HT1 and its use for novel microbial chryseobacterium camellia with keratinizing activity

Disclosed herein is a novel microbial chryseobacterium camellia having keratinizing activity and Dol-HT1 and its use.

Generally, the skin is classified into three parts from the outside of the skin: epidermis, dermis and subcutaneous fat layer. The double epidermis is subdivided into stratum corneum, granule layer, polar layer, and basal layer. The epidermal cells of basal layer are differentiated as they rise to the upper part of skin and finally reach the stratum corneum. The epidermal cells that reach the stratum corneum lose their nuclei and are converted to dead cells as they are filled with insoluble proteins called keratin.

Although keratinocytes are made in turn, they maintain a constant thickness because the old keratinocytes in the outermost layer are dissociated, and this process of turning new cells up to the stratum corneum and becoming dead cells is called turnover. In normal skin, the stratum corneum is composed of 15 to 20 layers, and it takes 15 to 20 days for them to be completely separated (British J. of Dermatology, 86, 14-19, 1974; KMHalprin, Perfume Society, 12 (4), 265-271, 1988), so that one layer of keratinocytes falls off per day.

If the stratum corneum does not peel off normally and remains on the surface of the skin, the stratum corneum becomes thicker and the face looks dull and dark. In addition, impurities remaining on the skin surface or hair follicles of hair are oxidized or decomposed by oxygen or microorganisms, causing skin problems such as inflammation. The main component of these impurities is keratin, which remains on the skin's surface even after the cell dies.

Keratin is large in molecular weight, insoluble and poorly decomposed, so it is not easily removed by the cleaning agent alone, but can be effectively removed by small decomposition using an enzyme. Using this stable protease can make the skin softer by removing the keratin layer attached to the skin surface.

Previously developed keratinase (keratinase) was mostly obtained from microorganisms selected from animal sources such as chicken feathers and poultry waste. Therefore, the development of plant or soil-derived keratinase is required for the development of eco-friendly cosmetic raw material. Although there have been reports of green tea-derived microorganisms, there has been no report on the excellent keratinase activity of the microorganisms. Related prior art documents include Korean Laid-Open Patent Publication No. 10-2001-0035745.

In one aspect, the present disclosure aims to provide a novel microorganism having keratinizing activity.

In another aspect, the present disclosure aims to provide a cosmetic composition for exfoliation.

In one aspect, the techniques disclosed herein are Chryseobacterium camellia zinnia-HT1 ( Chryseobacterium) camelliae Dolsongi-HT1) strain (Accession Number: KCCCM11883P).

In an exemplary embodiment, the strain may be a strain derived from green tea.

In an exemplary embodiment, the strain may be one having keratinizing activity.

In an exemplary embodiment, the strain may be one having keratinizing activity at pH 6-9.

In an exemplary embodiment, the strain may be one having a keratin degradation activity at pH 6 having an activity of 50 to 60% based on the keratin degradation activity at pH 9.

In an exemplary embodiment, the strain may be one having keratinizing activity at a temperature of 35 to 65 ℃.

In an exemplary embodiment, the strain may be one having a keratin degradation activity at 35 to 40 ° C of 75 to 85% based on the keratin degradation activity at 50 ° C.

In another aspect, the techniques disclosed herein provide keratinase isolated from the strain.

In an exemplary embodiment, the keratinase may be isolated from the culture of the strain, the lysate of the strain or the lysate of the culture.

In another aspect, the technology disclosed herein is a Chryseobacterium plant-derived Chryseobacterium camelliae ) strain, its culture or keratin derived from the strain to provide a cosmetic composition for exfoliation comprising an active ingredient.

In another aspect, the techniques disclosed herein provide an effective amount of Chryseobacterium camelia ( Chryseobacterium) for exfoliation. camelliae ) strain, its culture or keratinase derived from said strain is provided to a subject in need thereof.

In another aspect, the techniques disclosed herein are Chryseobacterium for exfoliating a subject. camelliae ) strain, its culture or keratinase derived from said strain.

In another aspect, the techniques disclosed herein provide the use of exfoliation of Chryseobacterium camelliae strains, cultures thereof, or keratinase derived from said strains.

In another aspect, the techniques disclosed herein are Chryseobacterium for exfoliating a subject. camelliae ) strains, cultures thereof, or uses for preparing a keratinase-containing composition from said strain.

In an exemplary embodiment, the Chryseobacterium camelliae strain, its culture, or keratinase derived from the strain may be applied or applied to a subject in the form of a cosmetic composition or an external skin composition. .

In an exemplary embodiment, the Chryseobacterium camelliae strain, its culture or keratinase derived from the strain may be applied or applied to the skin or scalp of the subject.

In an exemplary embodiment, the cryeobacterium camellia may be a strain derived from green tea.

In an exemplary embodiment, the chryseobacterium camellia may be Chryseobacterium camelliae Dolsongi-HT1, which is a chryseobacterium camellia with accession number KCCCM11883P having keratin degrading activity.

In an exemplary embodiment, the composition may be one having keratinizing activity.

In an exemplary embodiment, the composition may be one having keratinizing activity at pH 6-9.

In an exemplary embodiment, the composition may be one having a keratin degradation activity at pH 6 having an activity of 50 to 60% based on the keratin degradation activity at pH 9.

In an exemplary embodiment, the composition may be one having keratinizing activity at a temperature of 35 to 65 ℃.

In an exemplary embodiment, the composition may be one having a keratin degradation activity at 35 to 40 ℃ having an activity of 75 to 85% based on the keratin degradation activity at 50 ℃.

In one aspect, the techniques disclosed herein have the effect of providing novel microorganisms having keratinizing activity.

In another aspect, the techniques disclosed herein have the effect of providing a cosmetic composition having a keratin care use to exfoliate and trim the skin.

1 Chryseobacterium 16S rDNA subsequence of camelliae Dolsongi-HT1 is shown.

2 Chryseobacterium It shows the temperature dependent activity of keratinase produced from camelliae Dolsongi-HT1.

3 Chryseobacterium It shows the pH-dependent activity of keratinase produced from camelliae Dolsongi-HT1.

4 Chryseobacterium The results of comparing keratinase activity of camelliae Dolsongi-HT1 strain and Chryseobacterium camelliae THG C4-1 strain.

Hereinafter, the present invention will be described in detail.

The present specification provides a novel strain having keratinizing activity, a culture of said strain having keratinizing activity, and keratinase isolated from said strain.

In one aspect, the techniques disclosed herein include Cercybacterium camellia zinnia-HT1 (Chryseobacterium camelliae Dolsongi-HT1) strain (Accession Number: KCCCM11883P).

The microorganism according to the present specification may be improved or improved to have equivalent activity or better activity by the conventional physicochemical mutation method known in the art.

In an exemplary embodiment, the strain may be a strain derived from green tea.

In an exemplary embodiment, the strain may be one having keratinizing activity.

In an exemplary embodiment, the strain may be to produce keratinase in a culture medium containing 0.5% (w / v) keratin.

In an exemplary embodiment, the strain may be one having keratinizing activity at pH 6-9. Specifically, the strain is pH 6 or more, pH 6.5 or more, pH 7.0 or more, pH 7.5 or more, pH 8.0 or more, or pH 8.5 or more and pH 9.0 or less, pH 8.5 or less, pH 8.0 or less, pH 7.5 or less, pH 7.0 or less Or keratin-degrading activity at pH 6.5 or lower.

In one exemplary embodiment, the strain has a keratinizing activity at pH 6 of 50 to 60%, more specifically 52 to 58%, 54 to 58%, 55 to 57, based on the keratinizing activity at pH 9 It may have an activity of% or 56%.

In an exemplary embodiment, the strain may be one having keratinizing activity at a temperature of 35 to 65 ℃. Specifically, the strain is at least 35 ℃, at least 36 ℃, at least 37 ℃, at least 38 ℃, at least 39 ℃, at least 40 ℃, at least 42 ℃, at least 44 ℃, at least 46 ℃, at least 48 ℃, or at least 50 ℃ And keratin decomposition at temperatures of 65 ° C., 64 ° C., 63 ° C., 62 ° C., 61 ° C., 60 ° C., 58 ° C., 56 ° C., 54 ° C., 52 ° C. or less, or 50 ° C. or less. It may be one having activity.

In an exemplary embodiment, the strain may be one having a keratin degradation activity at 35 to 40 ° C of 75 to 85% based on the keratin degradation activity at 50 ° C. Specifically, 75% or more, 76% or more, 77% or more, 78% or more, 79% or more, or 80% or more and 85% or less, 84% or less, 83% or less, 82% or less, 81% or less, or 80 It may have an activity of less than or equal to%.

In an exemplary embodiment, the strain may be one having a keratin degradation activity at 36 to 39 ° C 75 to 85% activity based on the keratin degradation activity at 50 ° C.

In an exemplary embodiment, the strain may be one having a keratin degradation activity at 36 to 37 ° C 75 to 85% activity based on the keratin degradation activity at 50 ° C.

In an exemplary embodiment, the strain may be one having a keratin degradation activity at 37 to 38 ° C 75 to 85% activity based on the keratin degradation activity at 50 ° C.

In an exemplary embodiment, the strain may be one having a keratin degradation activity at 37 ° C of 75 to 85% based on the keratin degradation activity at 50 ° C.

In another aspect, the technology disclosed herein provides a exfoliation cosmetic composition comprising Chryseobacterium camelliae as an active ingredient.

In another aspect, the technology disclosed herein provides a cosmetic composition for exfoliation comprising Chryseobacterium camelliae culture as an active ingredient.

In another aspect, the technology disclosed herein provides a cosmetic composition for exfoliation comprising chryseobacterium camelliae derived keratinase as an active ingredient.

In an exemplary embodiment, the cryeobacterium camellia may be a strain derived from green tea.

In an exemplary embodiment, the Chryseobacterium camellia may be Chryseobacterium camelliae Dolsongi-HT1 (Cryseobacterium camelliae Dolsongi-HT1) having the accession number KCCCM11883P.

In one exemplary embodiment, the culture is the culture medium itself cultured in a suitable liquid medium according to the present specification, the filtrate (filtered or centrifuged supernatant) from which the strain is removed by filtration or centrifugation of the culture medium, the culture solution It may include, but is not limited to, cell disruption fluid obtained by sonicating or treating lysozyme in the culture, extracts of the culture, and the like. In one aspect, the culture may be preferably a filtrate of the culture or a culture supernatant centrifuged from the culture.

In an exemplary embodiment, the composition may be one having keratinizing activity.

In an exemplary embodiment, the composition may be one having keratinizing activity at pH 6-9. Specifically, the composition is pH 6 or more, pH 6.5 or more, pH 7.0 or more, pH 7.5 or more, pH 8.0 or more, or pH 8.5 or more and pH 9.0 or less, pH 8.5 or less, pH 8.0 or less, pH 7.5 or less, pH 7.0 or less Or keratin-degrading activity at pH 6.5 or lower.

In an exemplary embodiment, the composition has a keratinizing activity at pH 6 of 50 to 60%, more specifically 52 to 58%, 54 to 58%, 55 to 57, based on the keratinizing activity at pH 9 It may have an activity of% or 56%.

In an exemplary embodiment, the composition may be one having keratinizing activity at a temperature of 35 to 65 ℃. Specifically, the composition is at least 35 ℃, at least 36 ℃, at least 37 ℃, at least 38 ℃, at least 39 ℃, at least 40 ℃, at least 42 ℃, at least 44 ℃, at least 46 ℃, at least 48 ℃, or at least 50 ℃ And keratin decomposition at temperatures of 65 ° C., 64 ° C., 63 ° C., 62 ° C., 61 ° C., 60 ° C., 58 ° C., 56 ° C., 54 ° C., 52 ° C. or less, or 50 ° C. or less. It may be one having activity.

In an exemplary embodiment, the composition may be one having a keratin degradation activity at 35 to 40 ℃ having an activity of 75 to 85% based on the keratin degradation activity at 50 ℃. Specifically, 75% or more, 76% or more, 77% or more, 78% or more, 79% or more, or 80% or more and 85% or less, 84% or less, 83% or less, 82% or less, 81% or less, or 80 It may have an activity of less than or equal to%.

In an exemplary embodiment, the composition may be one having a keratin degradation activity at 36 to 39 ° C 75 to 85% activity based on the keratin degradation activity at 50 ° C.

In an exemplary embodiment, the composition may be one having a keratin degradation activity at 36 to 37 ℃ having an activity of 75 to 85% based on the keratin degradation activity at 50 ℃.

In an exemplary embodiment, the composition may be one having a keratin degradation activity at 37 to 38 ° C 75 to 85% activity based on the keratin degradation activity at 50 ° C.

In an exemplary embodiment, the composition may be one having a keratin degradation activity at 37 ℃ having an activity of 75 to 85% based on the keratin degradation activity at 50 ℃.

In an exemplary embodiment, the keratinase may be isolated from the culture to Chrysobacterium camellia.

In an exemplary embodiment, the active ingredient may be included in 0.001 to 10% by weight based on the total weight of the composition.

Chryseobacterium camelliae Dolsongi-HT1 ( Chryseobacterium camelliae Dolsongi-HT1), a culture thereof, and keratinase isolated therefrom derived from green tea according to the present specification are useful as raw materials for eco-friendly cosmetics derived from plants Compared with other keratinases in the related art, it exhibits excellent activity even at low temperature conditions compared to the high acidity conditions showing the optimum activity of the weak acidic conditions and keratinase, showing a specific effect by the origin of separation.

Specifically, compared to the characteristics of conventional keratinase, even in slightly acidic conditions have an activity of about 56% when compared to the optimum pH conditions (alkali conditions), temperature conditions of 37 ℃ (most keratinase Shows optimum activity at high temperature) and has an activity of about 80% or more when compared to 50 ° C., which is an optimum temperature condition.

In addition to the active ingredient, the cosmetic composition may further include functional additives and components included in the general cosmetic composition. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract. In addition to the other components included, oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation A promoter, a cooling agent, a restriction | limiting agent, purified water, etc. are mentioned.

The cosmetic composition is not particularly limited in formulation, and may be appropriately selected as desired. For example, Powder, Skin Lotion, Skin Softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nutrition Cream, Moisture Cream, Hand Cream, Foundation, Essence, Nutrition Essence, Pack, Shampoo , Soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser may be prepared in any one or more of the formulation selected from, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like can be used as carrier components. have.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Test Example 1 Isolation of Microorganisms from Green Tea

To screen for microorganisms with plant-derived keratin-degrading activity, liquid minimal media using keratin as a carbon and nitrogen source (M9 medium containing 0.5% soluble keratin: 0.015 g / L CaCl ₂ , 6.78 g / Na ₂ HPO ₄ ) Microorganisms were incubated at 30 ° C. or 37 ° C. using L, KH ₂ PO ₄ 3 g / L, NaCl 0.5 g / L, MgSO ₄ 0.5 g / L).

The microorganism screening method using the minimum medium according to this test method is a method for screening the microorganism of interest according to the growth rate of the microorganism, microorganisms with low keratinase activity is spontaneously removed in the cultivation step so Active microorganisms can be selected. Microorganisms capable of growing in minimal media have keratinase activity.

Accordingly, microorganisms having keratin-degrading activity were isolated from green tea of Dolsong tea garden in Jeju Island, Korea in a limited medium.

Test Example 2 Identification of Isolated Microorganisms

In order to identify the microorganisms having the keratinase activity isolated in Test Example 1, the DNA sequence encoding the microbial 16S ribosomal RNA partial sequence was analyzed to determine the species and genus.

After culturing the microorganism in a liquid medium, genomic DNA was extracted from the microorganism, and 27F (5'-AGAGTTTGATCMTGGCTCAG-3 ', SEQ ID NO: 2) / 1492R (5'-TACGGYTACCTTGTTACGACTT-3'), which is generally used for bacterial identification 3) The primer was used to amplify by PCR (polymerase chain reaction) method and its sequence was analyzed.

Sequencing Results, Chryseobacterium camelliae strain THG C4-1 (KACC 16985; Sequence accession no. (16S rRNA) JX843771) 99% homology with 16S rRNA subsequence, Chryseobacterium It was found to have 98% homology with the taiwanense 16S rRNA subsequence .

Thus, were isolated and cultured novel microorganism strain herein, through the molecular Phylogenetic analysis based on 16S rDNA base sequence to confirm that the Cri Seo tumefaciens Camellia (Chryseobacterium camelliae), (Chryseobacterium this Creations Seo tumefaciens Camellia camelliae ) Dolsongi-HT1, and the new microbial strain was deposited on September 01, 2016 to the depositing institution Korea Microorganism Conservation Center (KCCM) under the accession number KCCM11883P. Chryseobacterium of SEQ ID NO: 1 camelliae shown in Dolsongi-HT1 16S rDNA partial sequence 1 FIG.

Test Example  3. Chrysobacterium Camellia Stone -HT1 ( Chryseobacterium  camelliae  Characterization of Dolsongi-HT1)

The activity of keratinase produced from the novel strains identified in Test Example 2 was analyzed according to pH and temperature conditions.

The keratinase sample was inoculated with microorganisms in Nutrient broth liquid medium and incubated at 30 ° C. for about 20 hours, followed by centrifugation at 5,000 rpm to remove strains and to obtain a culture solution. The activity of keratinase was analyzed at pH ranging from 5 to 10 and temperature ranging from 30 to 60 ° C., and activity of keratinase was measured using keratin azure as a substrate. The keratin azuri turns azure dye upon decomposition by keratinase, and the reaction solution turns blue, and the activity can be compared by measuring the absorbance at 595 nm wavelength.

Specifically, the buffer and the sample in the pH range of 5 to 10 was mixed at 1: 1 (v / v), mixed with the keratin azuri substrate, and reacted with stirring at 37 ° C. for about 10 hours. At this time, keratin azuri was added at about 0.5% (w / v). In the case of the test according to the temperature change, the buffer and the sample were mixed 1: 1 (v / v) using pH 9 tris buffer, mixed with the keratin azuri substrate, and reacted with stirring at each temperature for about 10 hours.

Chryseobacterium camellia camelliae ) As a result of the keratinase activity analysis derived from Dolsongi-HT1, it showed the highest activity at a high temperature of 50 ° C. like other keratinases, and showed more than 80% of the activity even at 37 ° C. (See Figure 2). In the case of pH, as in other keratinase, it was confirmed that the activity was the highest under alkaline conditions of pH 9. In addition, in general, keratinase in the acidic conditions compared to the optimum alkaline conditions, the activity is very low, whereas in the case of keratinase according to the present specification about 56 compared to the optimum activity in the slightly acidic conditions of pH 6 It was confirmed that the activity of about% (see Fig. 3). pH 5 to 6 is a pH condition generally used in the preparation of cosmetic formulations, and thus it can be seen that keratinase according to the present specification is very useful as a raw material of cosmetics.

Test Example 4 Comparison of Keratase Activity

Chryseobacterium camellia identified in Test Example 2 Chryseobacterium camelliae Dolsongi-HT1 strain and Chryseobacterium camellia THG C4-1 ( Chryseobacterium Keratinase activity of camelliae THG C4-1) strain was compared as follows.

The keratinase samples were inoculated with the strains in LB (Luria-Bertani) liquid medium, and incubated at 30 ° C. for about 20 hours, followed by centrifugation at 5,000 rpm to remove the strains and to obtain a culture solution. The activity of keratinase was analyzed in the same manner as in Test Example 3 at pH 8 and temperature 37 ° C. Specifically, the pH 8 buffer and the sample were mixed at 1: 1 (v / v), mixed with the keratin azuri substrate, and reacted with stirring at 37 ° C. for about 10 hours.

As a result, as shown in FIG. 4, the Cryogenic bacterium camellia-HT1 strain exhibited about 1.8 times higher keratinase activity in the Cryeobacterium camellia than the THG C4-1 strain. It was confirmed that the Dolsong-HT1 strain had a very good effect compared to the same strain.

Examples of the formulation of the composition according to one aspect of the present specification will be described below, but can be applied to various other formulations, which are intended to explain in detail only and not intended to limit the present invention.

[ Formulation example  1] Flexible Cosmetic (Skin Lotion)

Compounding ingredient Content (% by weight) Strain or culture thereof 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[ Formulation example  2] Nutritional Cosmetics ( Milk Croissant )

Compounding ingredient Content (% by weight) Strain or culture thereof 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Beeswax 4.0 Polysorbate 60 1.5 Caprylic / Capric Triglycerides 5.0 Squalane 5.0 Sorbitassquioleate 1.5 Liquid paraffin 0.5 Cetearyl Alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[ Formulation example  3] Nutrition Cream

Compounding ingredient Content (% by weight) Strain or culture thereof 0.1 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[ Formulation example  4] Massage Cream

Compounding ingredient Content (% by weight) Strain or culture thereof 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Beeswax 4.0 Cetearyl Glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[ Formulation example  5] pack

Compounding ingredient Content (% by weight) Strain or culture thereof 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl Phenyl Ether 0.4 Polysorbate 60 1.2 Ethanol preservative Quantity Preservative, coloring, flavoring Quantity Purified water Remaining amount

As described above, specific portions of the present disclosure have been described in detail, and for those skilled in the art, these specific techniques are merely preferred embodiments, and the scope of the present disclosure is not limited thereto. Will be obvious. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

[Accession number]

Depositary Name: Korea Microorganism Conservation Center

Accession number: KCCM11883P

Trust Date: 20160901

[Microbial deposit]

Claims (10)

Cryeobacterium camellia zinnia-HT1 ( Chryseobacterium camelliae Dolsongi-HT1) strain (Accession Number: KCCCM11883P). The method of claim 1, The strain is a strain derived from green tea. The method of claim 1, The strain has keratinizing activity. The method of claim 1, The strain is one having a keratinizing activity at pH 6 to 9. The method of claim 1, Wherein said strain has a keratin cleavage activity at pH 6 having an activity of 50 to 60% based on keratin breakdown activity at pH 9. The method of claim 1, The strain is one having a keratinizing activity at a temperature of 35 to 65 ℃. The method of claim 1, Wherein said strain has a keratin cleavage activity at 35 to 40 ° C. having an activity of 75 to 85% based on keratin breakdown activity at 50 ° C. 6. Keratinase isolated from the strain of any one of claims 1 to 7. Chryseobacterium camellia from plant origin camelliae ) strain, a culture thereof or a cosmetic composition for exfoliation comprising keratinase derived from the strain as an active ingredient. The method of claim 9, The cryeobacterium camellia strain is the strain of any one of claims 1 to 7, wherein the composition.

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