WO2018062751A1 - Composition containing lysate of lactobacillus plantarum for hair or scalp - Google Patents

Abstract

The present specification describes a composition containing a lysate of Lactobacillus plantarum as an active ingredient for hair or scalp. The composition can exhibit remarkably excellent hair growth promoting and hair loss preventing effects.

Description

Composition for hair or scalp comprising eluate of Lactobacillus plantarum

This specification relates to a composition for hair or scalp comprising an eluate of Lactobacillus plantarum.

In recent years, there is a demand for materials derived from nature according to the trend of avoiding chemical raw materials, insects and animal raw materials. The microbial resource is a highly useful material that utilizes the unique characteristics of microorganisms adapted in various environments as a sustainable resource in terms of reproduction. Among these, lactic acid bacteria are used as probiotics in the food field as materials that can be used without risk to the human body.

In one aspect, the problem to be solved by the present invention is to provide a composition having excellent hair growth and hair loss prevention efficacy.

In another aspect, the problem to be solved by the present invention is to provide a composition having excellent hair growth and hair loss prevention efficacy without human toxicity or irritation.

In another aspect, the problem to be solved by the present invention is to provide a composition having excellent hair growth enhancement and hair loss prevention effect using a component derived from nature.

In another aspect, the problem to be solved by the present invention is to provide a composition excellent in promoting the proliferation of hair follicle dermal papilla cells, and suppresses the death thereof.

In another aspect, the problem to be solved by the present invention is to provide a composition excellent in promoting the proliferation of appearance myocytes, and suppresses the death.

In one aspect, the present invention relates to a hair or scalp composition comprising an lysate of Lactobacillus plantarum as an active ingredient.

In another aspect, the present invention (a) Lactobacillus plantarum ( Lactobacillus plantarum ) is cultured,

(b) centrifuging the cultured Lactobacillus plantarum to remove insoluble components, and

(c) a hair comprising the eluate of Lactobacillus plantarum, which is eluted by heating the Lactobacillus plantarum obtained in (b) at 80 ° C to 120 ° C for 10 to 30 minutes, or It relates to a method for preparing a scalp composition.

In one aspect, the present invention can provide a composition excellent in hair growth promotion and hair loss prevention efficacy.

In another aspect, the present invention can provide a composition having excellent hair growth enhancement and hair loss prevention efficacy without human toxicity or irritation.

In another aspect, the present invention can provide excellent hair growth promotion and hair loss prevention efficacy by using ingredients derived from nature.

In another aspect, the present invention can provide a composition excellent in promoting the proliferation of hair follicle dermal papilla cells and inhibiting the death thereof.

In another aspect, the present invention can provide an excellent composition for promoting the proliferation of appearance myocytes and inhibiting their death.

1 is a graph showing the hair follicle dermal papilla cell growth promoting effect of Lactobacillus plantarum.

Figure 2 is a result graph showing the effect of Lactobacillus plantarum promotes the appearance of myocyte root proliferation.

Figure 3 is a result graph showing the effect of Lactobacillus plantarum inhibits the appearance of myocytes.

Figure 4 is a fluorescence micrograph showing the effect of Lactobacillus plantarum inhibiting appearance myocytes cell death.

Hereinafter, with reference to the accompanying drawings, it will be described embodiments of the present application in more detail. However, the technology disclosed in the present application is not limited to the embodiments described herein and may be embodied in other forms. It is merely to be understood that the embodiments introduced herein are provided so that the disclosure can be made thorough and complete, and that the spirit of the present application can be fully conveyed to those skilled in the art. In order to clearly express each component in the drawings, the size, such as the width or thickness of the component, is shown to be somewhat enlarged. In addition, although only a part of the components are shown for convenience of description, those skilled in the art will be able to easily understand the rest of the components. In addition, one of ordinary skill in the art may implement the spirit of the present application in various other forms without departing from the technical spirit of the present application.

In one embodiment of the present invention, it is possible to provide a composition for hair or scalp comprising an lysate of Lactobacillus plantarum as an active ingredient.

In one embodiment, the Lactobacillus plantarum may be derived from a tea tree. In one embodiment, the Lactobacillus plantarum is Camellisa sinensis ) may be isolated from the leaves.

In one embodiment, the Lactobacillus plantarum may be an APsulloc 331266 (Accession No. KCCM11181P) strain. The strain may be identified and isolated from the tea leaves, it may exhibit a remarkably excellent hair growth and hair loss prevention effect among the various Lactobacillus plantarum strains isolated and identified from the tea leaves.

In one embodiment, the eluate may be a heated eluate obtained by heating and eluting cells of Lactobacillus plantarum. For example, the heating is 80 ° C or higher, 81 ° C or higher, 82 ° C or higher, 83 ° C or higher, 84 ° C or higher, 85 ° C or higher, 86 ° C or higher, 87 ° C or higher, 88 ° C or higher, 89 ° C or higher, 90 ° C or higher, 91 degrees C or more, 92 degrees C or more, 93 degrees C or more, 94 degrees C or more, 95 degrees C or more, 96 degrees C or more, 97 degrees C or more, 98 degrees C or more, 99 degrees C or more, or 100 degrees C or more, 120 degrees C or less, 119 degrees C or less, 118 Or less, 117 or less, 116 or less, 115 or less, 114 or less, 113 or less, 112 or less, 111 or less, 110 or less, 109 or less, 108 or less, 107 or less, or 106 or less , 105 ° C. or less, 104 ° C. or less, 103 ° C. or less, 102 ° C. or less, or 101 ° C. or less. For example, the heating may be at least 5 minutes, at least 6 minutes, at least 7 minutes, at least 8 minutes, at least 9 minutes, at least 10 minutes, at least 11 minutes, at least 12 minutes, at least 13 minutes, at least 14 minutes, or at least 15 minutes. 30 minutes or less, 29 minutes or less, 28 minutes or less, 27 minutes or less, 26 minutes or less, 25 minutes or less, 24 minutes or less, 23 minutes or less, 22 minutes or less, 21 minutes or less, 20 minutes or less, 19 minutes or less, 18 minutes or less, 16 minutes or less, or 15 minutes or less. The heated eluate is a natural ingredient, there is little irritation or toxicity when applied to the human body, hair growth enhancement and hair loss prevention effect can be remarkably excellent.

In one embodiment, the eluate is (a) Lactobacillus plantarum culture ( Lactobacillus plantarum ),

(b) centrifuging the cultured Lactobacillus plantarum to remove insoluble components, and

(c) may be prepared by eluting the Lactobacillus plantarum obtained in (b) by heating at 80 to 120 ℃ for 10 to 30 minutes.

The composition according to an embodiment of the present invention is excellent in safety because it is almost no irritation or toxicity when applied to the human body as a natural component. In addition, it promotes proliferation of hair follicle dermal papilla cells and hair follicle myocytes, inhibits the death of the hair follicles, and can exhibit remarkably excellent hair growth promoting effect and hair loss prevention effect.

In one embodiment of the present invention, the composition may be a topical skin composition.

The external preparation composition may be, for example, a cosmetic composition.

Compositions according to one embodiment of the present invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and non-obtained by dispersing an oil phase in solution, gels, solids, pasty anhydrous products, aqueous phases. It may be provided in the form of an ionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions can be prepared according to conventional methods in the art.

In addition, the composition according to an embodiment of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

In one example, the cosmetic composition may include the Lactobacillus plantarum eluate 0.1% to 20% by weight relative to the total weight of the composition.

In one embodiment, the composition may be used as a scalp or hair composition. Therefore, the composition may be used in combination with the scalp and hair, such as shampoos, rinses, scalp treatments, hair treatments, scalp hair treatments, hair loss treatments, damaged hair treatments, and leave-in. ) Scalp or hair, scalp and hair combination products, such as conditioner, wash-off conditioner or hair essence.

The composition of each of these formulations may contain various components formulated into conventional scalp or hair compositions according to the various formulations described above or as appropriate for the end purpose, and the types and amounts of these components can be easily selected by those skilled in the art. .

In one embodiment, the composition may comprise 0.1 to 20% by weight of the Lactobacillus plantarum eluate relative to the total weight of the composition.

In addition, the composition according to the embodiment of the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, and an ionic type. Or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other commonly used in cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological fields, such as ingredients. The adjuvant may be introduced in amounts generally used in the cosmetic or dermatological arts, for example from 0.001% to 5% by weight, for example from 0.001 to 3% by weight relative to the total weight of the composition.

In one embodiment of the present invention, the composition may be a pharmaceutical composition.

The pharmaceutical composition according to the present disclosure may be in various oral or parenteral formulations. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, or the like. Or lactose, gelatin, or the like is mixed. In addition to simple excipients, lubricants such as magnesium stearate, talc and the like are also used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups, and various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin, may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Pharmaceutical dosage forms of the compositions herein may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection. The salt is not particularly limited as long as it is pharmaceutically acceptable, and for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid , Benzene sulfonic acid, toluene sulfonic acid, naphthalene sulfonic acid and the like can be used.

The composition of the present disclosure may be parenterally or orally administered as desired, and may be administered in one to several times so as to be administered in an amount of 0.1 to 500 mg, preferably 1 to 100 mg per kg of body weight per day. have. The dosage for a particular patient may vary depending on the patient's weight, age, sex, health condition, diet, time of administration, method of administration, rate of excretion, severity of the disease, and the like.

The pharmaceutical composition according to the present specification may be a powder, granules, tablets, soft or hard capsules, suspensions, emulsions, syrups, oral formulations such as aerosols, ointments, creams, etc. And sterile injectable solutions and the like, and may be formulated in any form suitable for pharmaceutical preparations.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Preparation Example 1 Preparation of Lactobacillus Planta Room APsulloc 331266 Eluate

1. Isolation of Lactobacillus plantarum strains

200 g of the tea leaves were washed twice with distilled water to remove foreign substances, the water of the washed tea leaves were shaken off and mixed with the salt corresponding to 8% of the weight of the tea leaves and left at room temperature for 3 hours. The salted tea leaves were mixed with 1000 ml of a 1% fructo oligosaccharide solution and incubated in a 32 ° C. incubator for 3 days. After 3 days, the pH of the culture medium was checked to be lower than 5, and when the pH was lower than 5, it was taken and incubated in Difco Lactobacilli MRS Agar® medium. At this time, the culture was carried out for 2 days in 32 ℃, anaerobic chamber after taking a colony showing a colony of white, Lactobacillus plantarum APsulloc 331266 (Accession Number: KCCM11181P) was isolated from the tea leaves in the same manner as above.

2. Culture and Elution of Lactobacillus Plantarum

The isolated Lactobacillus plantarum APsulloc 331266 was stored in Cryotubes at 10% by weight in skim milk, inoculated in medium (MRS Broth), and incubated in a 37 ° C. environment for 24 hours. When the bacteria concentration in the liquid medium was 5 X 10 ⁸ ~ 1 X 10 ⁹ CFU / ㎖, at 4 ° C, the feeder cells were harvested by centrifugation at 4000 rpm and washed with PBS (phosphate buffered saline). The washed cells were re-dispersed in PBS at a concentration of 5 × 10 ⁸ to 1 × 10 ⁹ CFU / mL and heated at 100 ° C. for 10 minutes to obtain an eluate.

Test 1 Proliferative effect of hair follicle papilla cells (MTT assay)

The eluate prepared in Preparation Example 1 was used to confirm the proliferation effect in human hair follicle papilla cells (hDPCs; human Dermal papilla Cell).

1. hDPCs isolation and culture

The dermal papilla cells were isolated from the scalp scalp tissue and cultured for 14 days in a culture dish coated with collagen type I. Culture medium containing DMEM (Dulbecco's modified eagles medium; HyClone GE Healthcare) with antibiotic (1%), fungizone (0.1%), 20% FBS, 5% CO _2, for 72 hours at 37 ° C After incubation, the medium was replaced with DMEM medium added with 10% FBS, and when the cells grew more than 90% of the dish, the cells were collected using 0.25% trypsin / 10mM EDTA (Welgene) and 10% FBS Incubated for 72 hours (until the cells grew more than 90% of the dish) in the DMEM medium, 5% CO _2, 37 ℃ added to provide the experiment.

2. Culture for Confirmation of hDPCs Cell Proliferation Effect

HDPCs were inoculated in 2000 wells / well in 96 well plates and incubated at 5% CO _2, 37 ° C for 24 hours. 10% by volume of the eluate prepared in Preparation Example 1 was incubated at 37% for 5% CO ₂ for 72 hours. A control group was used as a control group, and 5% FBS was treated with the same as the eluate as a positive control group.

3. Cell proliferation measurement: More than 120% viability compared to control

MTT solution (Sigma, 70 µg / well) was added to each well of the 96 well plate and incubated at 5% CO _2, 37 ° C. for 3 hours. Thereafter, formazan was dissolved in DMSO and absorbance was measured at 570 nm using a microplate reader. The relative cell proliferation rate is shown in FIG. 1 with a control value of 100 in the measured result.

In the results of Figure 1, it can be seen that the APsulloc 331266 strain of Preparation Example 1 shows an excellent hair follicle papilla cell proliferation effect.

[Test Example 2] Appearance myocyte proliferation effect

Using the eluate prepared in Preparation Example 1 was confirmed the proliferation effect of the outer root sheath keratinocyte (ORS keratinocyte).

1. Isolation and Culture of ORS keratinocytes

The superficial myoblasts were isolated from the scalp scalp tissue and cultured for 14 days in a culture dish coated with collagen type I. Culture medium containing DMEM (Dulbecco's modified eagles medium; HyClone GE Healthcare) with antibiotic (1%), fungizone (0.1%), 20% FBS, 5% CO ₂ for 3 days at 37 ° C Cultured, replaced with the appearance myocytes culture medium (EPILIFE MEPI500CA, invitrogen), and confirmed confluent and then subcultured by trypsin / EDTA (T / E) treatment.

2. Culture for Confirmation of Cell Proliferation Effect of Appearance Myocytes

After inoculating 20000 cells / well into 96 well plates, the cells were incubated at 5% CO _{2 and} 37 ° C. for 24 hours. 10% by volume of the eluate prepared in Preparation Example 1 was incubated at 37% for 5% CO ₂ for 72 hours. A control group was used as an untreated group, and as a positive control, the growth factor (EpiLife S-012-5, invitrogen) was added to the E. coli culture medium (EPILIFE MEPI500CA, invitrogen). It was.

3. Cell proliferation measurement: More than 120% viability compared to control

MTT solution (Sigma, 70 µg / well) was added to each well of the 96 well plate and incubated at 5% CO _2, 37 ° C. for 3 hours. Thereafter, formazan was dissolved in DMSO and absorbance was measured at 570 nm using a microplate reader. The relative cell proliferation rate is shown in FIG. 2 with the control value as 100 in the measured result.

In the results of Figure 2, the APsulloc 331266 strain of Preparation Example 1 can be seen to exhibit an excellent appearance myocytes proliferation effect.

Test Example 3 Inhibitory Effect of Appearance Myocytes

Using the eluate prepared in Preparation Example 1 was confirmed the effect of inhibiting the death of outer root sheath keratinocyte (ORS keratinocyte) induced by DKK-1 known as the hair loss causing factor.

1. Cell Culture

Appearance and myoblasts isolated and cultured as in Test Example 2 were inoculated at 20000 / well in a 96 well plate and 8 chamber slide incubated for 24 hours at 5% CO _2, 37 ℃ conditions. After 24 hours of starvation, the cells were treated with DKK 50ng / ml and DKK 50ng / ml + 10% by volume of the eluate prepared in Preparation Example 1, respectively. The growth factor (EpiLife S-012-5, invitrogen) was added to the E. coli culture medium (EPILIFE MEPI500CA, invitrogen) in the same amount as the eluate.

2. MTT assay

MTT solution (Sigma, 70 µg / well) was added to each well of the 96 well plate treated with the test material and incubated at 5% CO _2, 37 ° C. for 3 hours. Thereafter, formazan was dissolved in DMSO and absorbance was measured at 570 nm using a microplate reader. The results are shown in FIG. 3 as relative cell proliferation rate (***) with a negative control value of 100 and relative cell proliferation rate (†) with a value of 100 treated with DKK-1 only.

3.TUNEL assay (8 chamber slide)

The 8 chamber slide treated with the test substance was fixed for 24 hours using 4% paraformaldehyde (Paraformaldehyde), washed, and treated with triton-X100 0.05% (in PBS). After the reaction using a TUNEL reaction mixture (TUNEL reaction mixture, Roche kit) was washed, stained with DAPI (4 ', 6-diamidino-2-phenylindole) and observed under a fluorescence microscope. Fluorescence micrographs of the cells are shown in FIG. 4.

3 and 4, it can be confirmed that the APsulloc 331266 strain of Preparation Example 1 exhibits an excellent inhibitory effect on appearance myocytes.

Examples of the formulation of the composition according to one aspect of the present specification will be described below, but it is also applicable to various other formulations, which are intended to explain in detail only and not intended to limit the present specification.

Formulation Example 1 Tablet

100 mg of the eluate of Preparation Example 1 of the present invention, 400 mg of lactose, 400 mg of corn starch, and 2 mg of magnesium stearate were mixed, followed by tableting according to a conventional method for preparing tablets.

 Formulation Example 2 Capsule

100 mg of the eluate of Preparation Example 1 of the present invention, 400 mg of lactose, 400 mg of corn starch and 2 mg of magnesium stearate were mixed, followed by filling into a gelatin capsule according to a conventional capsule preparation method to prepare a capsule.

Formulation Example 3 Granules

50 mg of the eluate of Preparation Example 1 of the present invention, 250 mg of anhydrous glucose and 550 mg of starch were mixed, molded into granules using a fluidized bed granulator, and then filled into fabrics.

[Formulation Example 4] Drinks

50 mg of the eluate of Preparation Example 1 of the present invention, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharides were mixed, and 300 ml of purified water was added thereto, and 200 ml were filled in each bottle. After filling the bottle sterilized for 4-5 seconds at 130 ℃ to prepare a drink.

Formulation Example 5 Injection

Injections were prepared by conventional methods according to the compositions set forth in the table below.

Compounding ingredient content Eluate of Preparation Example 1 10-50 mg Sterile Distilled Water for Injection Quantity pH regulator Quantity

 Formulation Example 6 Softener (Skin Lotion)

According to the composition shown in the following table was prepared in the conventional method for the flexible cosmetic.

Compounding ingredient Content (% by weight) Eluate of Preparation Example 1 0.2 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 7 Nutrients (Milk Lotion)

Nutrients were prepared in a conventional manner according to the composition shown in the table below.

Compounding ingredient Content (% by weight) Eluate of Preparation Example 1 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Beeswax 4.0 Polysorbate 60 1.5 Caprylic / Capric Triglycerides 5.0 Squalane 5.0 Sorbitassquioleate 1.5 Liquid paraffin 0.5 Cetearyl Alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 8 Massage Cream

Massage cream was prepared in a conventional manner according to the composition described in the table below.

Compounding ingredient Content (% by weight) Eluate of Preparation Example 1 2.0 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Beeswax 4.0 Cetearyl Glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Formulation Example 9 Preparation of Hair Lotion

A hair lotion is prepared according to a conventional method with the composition described in the table below.

ingredient Content (% by weight) Eluate of Preparation Example 1 2.0 Purified water Remaining amount glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Edelweiss extract 2.0 Caprylic Capric Triglycerides 3.0 Squalene 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 1.2 antiseptic Quantity incense Quantity Pigment Quantity Triethanol amine 0.1

Formulation Example 10 Preparation of Shampoo Composition

A shampoo composition was prepared according to a conventional method with the compositions described in the table below.

Ingredient (% by weight) Content (% by weight) Polyquaternium-10 0.5 Sodium laureth sulfate 20 Eluate of Preparation Example 1 0.3 incense 1.0 Methylparaben 0.1 Cetyl alcohol 0.5 Sodium chloride 0.8 Purified water To 100

Formulation Example 11 Preparation of Hair Treatment

Hair treatments were prepared according to conventional methods with the compositions described in the table below.

Division (weight%) Content (% by weight) Eluate of Preparation Example 1 0.5 Stearamidopropyldimethylamine 2 glycerin 0.5 Spices 0.5 antiseptic 0.03 Cetostearyl alcohol 5 Lactic acid One Distilled water to 100

[Accession number]

Depositary Name: Korea Microorganism Conservation Center (overseas)

Accession number: KCCM11181P

Date of Trust: 2011.03.28

[Microbial deposit]

Claims (11)

Lactobacillus plantarum ( Lactobacillus plantarum ) A composition for hair or scalp comprising an lysate as an active ingredient. The method of claim 1, The Lactobacillus plantarum is a strain of Accession No. KCCM11181P, composition. The method according to claim 1 or 2, The Lactobacillus plantarum is isolated and identified from the tea tree, the composition. The method according to claim 1 or 2, The eluate of the Lactobacillus plantarum is obtained by heating and eluting cells of the Lactobacillus plantarum. The method of claim 4, wherein Wherein the heating is performed at 80 ° C. to 120 ° C. for 10 to 30 minutes. The method according to claim 1 or 2, The composition is for promoting hair growth or preventing hair loss. The method according to claim 1 or 2, The composition is for promoting the proliferation of hair follicle papilla cells and inhibiting death. The method according to claim 1 or 2, The composition is for promoting the growth of hair follicle myocytes and inhibiting death. The method according to claim 1 or 2, The composition is a cosmetic composition. The method according to claim 1 or 2, The composition is a pharmaceutical composition. (a) incubating Lactobacillus plantarum , (b) centrifuging the cultured Lactobacillus plantarum to remove insoluble components, and (c) a hair comprising the eluate of Lactobacillus plantarum, which is eluted by heating the Lactobacillus plantarum obtained in (b) at 80 ° C to 120 ° C for 10 to 30 minutes, or Method of producing a composition for scalp.

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