[go: up one dir, main page]

WO2018046929A9 - Methods and compositions for co-culturing pluripotent and extra-embryonic cells - Google Patents

Methods and compositions for co-culturing pluripotent and extra-embryonic cells Download PDF

Info

Publication number
WO2018046929A9
WO2018046929A9 PCT/GB2017/052619 GB2017052619W WO2018046929A9 WO 2018046929 A9 WO2018046929 A9 WO 2018046929A9 GB 2017052619 W GB2017052619 W GB 2017052619W WO 2018046929 A9 WO2018046929 A9 WO 2018046929A9
Authority
WO
WIPO (PCT)
Prior art keywords
methods
extra
compositions
embryonic cells
culturing pluripotent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2017/052619
Other languages
French (fr)
Other versions
WO2018046929A1 (en
Inventor
Magdalena Zernicka-Goetz
Sarah Ellys HARRISON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2018046929A1 publication Critical patent/WO2018046929A1/en
Publication of WO2018046929A9 publication Critical patent/WO2018046929A9/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C12N2501/392—Sexual steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/03—Coculture with; Conditioned medium produced by non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/56—Fibrin; Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to methods for culturing mammalian embryonic stem cells and culture media used in such methods.
PCT/GB2017/052619 2016-09-09 2017-09-07 Methods and compositions for co-culturing pluripotent and extra-embryonic cells Ceased WO2018046929A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1615343.9A GB201615343D0 (en) 2016-09-09 2016-09-09 Methods
GB1615343.9 2016-09-09

Publications (2)

Publication Number Publication Date
WO2018046929A1 WO2018046929A1 (en) 2018-03-15
WO2018046929A9 true WO2018046929A9 (en) 2018-07-05

Family

ID=57234628

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2017/052619 Ceased WO2018046929A1 (en) 2016-09-09 2017-09-07 Methods and compositions for co-culturing pluripotent and extra-embryonic cells

Country Status (2)

Country Link
GB (1) GB201615343D0 (en)
WO (1) WO2018046929A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109749987B (en) * 2019-01-18 2021-10-01 中国农业大学 A kind of method for preparing reconstructed embryo by suspension shaking co-cultivation and special composition thereof
EP4251735A4 (en) 2020-11-24 2025-02-26 Monash University Methods and cellular structures
CA3199729A1 (en) * 2020-11-24 2022-06-02 Monash University Induced stem cells
WO2023151475A1 (en) * 2022-02-10 2023-08-17 The University Of Hong Kong A method of derivation of mesenchymal stem cells from mammalian pluripotent stem cells
AU2023231520A1 (en) 2022-03-06 2024-10-24 Yeda Research And Development Co. Ltd. Methods of generating a synthetic embryo
WO2024261290A1 (en) * 2023-06-23 2024-12-26 Academisch Ziekenhuis Maastricht Method for the culture of embryo-like structures
WO2025060030A1 (en) * 2023-09-22 2025-03-27 苏州血霁生物科技有限公司 Method for culturing pluripotent stem cells and use thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0623635D0 (en) * 2006-11-27 2007-01-03 Stem Cell Sciences Uk Ltd Pluripotent cell growth media
WO2008153568A1 (en) * 2007-06-13 2008-12-18 Lifescan, Inc. Chorionic villus derived cells
KR100980005B1 (en) * 2008-02-21 2010-09-06 한국생명공학연구원 How to induce differentiation of embryonic stem cells into cardiomyocytes
US9102914B2 (en) * 2011-02-03 2015-08-11 Empire Technology Development Llc 3D trophoblast matrix for preparing organ-specific stem cells
CN105531365A (en) * 2013-04-23 2016-04-27 耶达研究及发展有限公司 Isolated naive pluripotent stem cells and methods of generating same
WO2015022541A1 (en) * 2013-08-15 2015-02-19 Cambridge Enterprise Limited Media and methods for culturing embryos and stem cells

Also Published As

Publication number Publication date
GB201615343D0 (en) 2016-10-26
WO2018046929A1 (en) 2018-03-15

Similar Documents

Publication Publication Date Title
WO2018046929A9 (en) Methods and compositions for co-culturing pluripotent and extra-embryonic cells
WO2016061071A8 (en) Generation of keratinocytes from pluripotent stem cells and maintenance of keratinocyte cultures
WO2015188106A3 (en) Perfusion culturing methods and uses thereof
MX2022003477A (en) Taurine supplemented cell culture medium and methods of use.
MA39867A (en) ISOLATION, CULTURE AND GENETIC MANIPULATION OF IMMUNE CELL POPULATIONS FOR ADOPTIVE THERAPY
MX2021008143A (en) Methods for harvesting mammalian cell cultures.
WO2015054526A3 (en) Methods of mammalian retinal stem cell production and applications
WO2016057951A3 (en) Crispr oligonucleotides and gene editing
MX2015012202A (en) Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions.
EA202091407A1 (en) COMPOSITION OF A SERUM-FREE MEDIUM FOR CELL CULTIVATION AND METHODS OF ITS APPLICATION
MX2019003427A (en) Cell culture compositions and methods for polypeptide production.
WO2016168890A8 (en) Generation of muscle-lineage cells from stem cells
WO2011021194A3 (en) Pericyte progenitor cells and methods of generating and using same
IL288819A (en) Cell culture methods and compositions for antibody production
WO2017015245A8 (en) Methods and compositions for stem cell transplantation
MX2016013223A (en) Compounded media powder formulation and method of preparation of liquid medium for cell culture.
IL288260A (en) Compositions and methods for plant cell culture
MX2018015234A (en) Methods to improve genetic transformation of sorghum.
WO2015175534A3 (en) Compositions and methods employing stem cell-derived cardiomyocytes
WO2015171852A3 (en) Methods and compositions for non-cytotoxic stem cell transplantation
MX2021001981A (en) Methods of preparing a poloxamer for use in cell culture medium.
MX2022000516A (en) Methods relating to pluripotent cells.
ZA202107808B (en) Cell culture medium for eukaryotic cells
WO2015117033A3 (en) Methods of increasing protein production in mammalian cells
WO2015006509A9 (en) Embryo culture methods and media

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17787520

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 17787520

Country of ref document: EP

Kind code of ref document: A1