WO2017101020A1 - Modified dressing - Google Patents

Abstract

A modified dressing, being formed by solidifying a mussel adhesive protein on a matrix dressing . Said dressing promotes the cell growth and shortens the healing time, while protecting a wound surface; moreover, after the mussel adhesive protein is solidified on the dressing, the physical and chemical stabilities of the protein are improved, extending the degradation period of the protein, facilitating the exhibition of the bioactivity of the mussel adhesive protein.

Description

An improved dressing Technical field

The present invention relates to an improved dressing and, more particularly, to a product for curing mussel mucin on a dressing.

Background technique

Dressings are medical materials used to cover wounds, cover sores, wounds or other damage during treatment, such as dressings, dressings for treating wounds, and the like. Conventional dressings generally act to cover the wound, have no effect on the healing process, and are ineffective against infected wounds. With the in-depth study of the pathophysiology of wound healing process, the wound dressings are continuously improved and developed. A variety of medical dressings with different properties can be used by clinical nurses, such as interactive dressings, calcium alginate dressings, foam dressings, hydrocolloids. Dressings, hydrogel dressings, etc. Modern dressings are functionally resistant to mechanical damage, resist pollution and chemical irritation, prevent secondary infections, prevent dryness and loss of body fluids, prevent heat loss, protect wounds, and create a microenvironment that promotes wound healing through debridement. For example, the hydrocolloid dressing uses a closed moisturizing principle, and the hydrophilic methylcellulose particles are in contact with the wound exudate liquid phase to form a moist gel on the surface of the wound. The hydrogel dressing is a high-water content hydrophilic polyurethane polymer, which automatically adjusts the wetness of the wound, has the ability to absorb the liquid and absorb the liquid, does not stick to the wound, and is easy to remove. However, in the promotion of healing, the dressing is more effective in creating a microenvironment such as humidity required for healing, and does not actively affect the healing process.

Mussel adhesive protein (MAP), also known as Mytilus edulis foot protein (Mefp), is a marine shellfish, Mytilus edulis Linnaeus, and a small shell mussel (Mytilus coruscus). A special protein secreted by Perna viridis. Mussels are usually attached in groups to the reefs on the coast or to the bottom of the ship, and have the ability to withstand wave impacts in the offshore. In fact, mussels can be attached extremely strongly to the substrate of any material, such as metal, wood, glass, and the like. The main reason for the above characteristics of mussels is that they can form and store this special mucin in the girth of the foot. The mussels release the mucin through the foot silk to a solid surface such as rock to form a water-resistant combination. Fix yourself.

Currently, 11 mucin subclasses have been identified from mussels, including mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen pre-COL-P, pre-COL-D. , pre-COL-NG, Foot silk matrix proteins PTMP and DTMP (Zhu et al., Advances in Marine Science, 2014, 32(4): 560-568). Mussel mucin has two structural features: (1) containing lysine, which has a high loading of positive charge; (2) containing 3,4 dihydroxyphenylalanine (DOPA, dopa). The cells and tissues of the human body are negatively charged. Mussel mucin binds to cells and tissues through the electrostatic interaction between its own positive charge and the negative charge of cells and tissues of the human body, promoting cell growth and exerting protective and therapeutic effects. In addition, dopa oxidation produces ortho-dioxins, which can be cross-linked with unoxidized dopa to form a membrane or a network scaffold, which promotes the protein to adhere more closely and firmly to the surface of the human body, thereby protecting. Mussel mucin is a macromolecular protein that is completely degraded in the human body for about 3-10 days. Its ability to attach to cell tissues is excellent, so that mussel mucin can be stabilized locally and continue to function.

At present, the application fields of mussel mucin products are very limited. Commercial mussel mucin products include Cell-Tak from BD Biosciences, MAP Trix from Kollodis, Korea, and Hydrogel from Biopolymer, Sweden. These products are either used directly in the mussel mucin solution state, or are stored as lyophilized powder formulations and dissolved prior to use. Their primary application is limited to microscopic cell adhesion and tissue adhesives. Mussel mucin has also been reported for use in membrane repair, as a coating against seawater corrosion.

Mussel mucin can be used in the wound healing process because it has the function of promoting cell adhesion and crawling. It is usually used alone with the dressing during use to accelerate the healing of the wound. However, due to factors such as exudation of the wound, multiple administrations are required to achieve a healing promoting effect.

Summary of the invention

SUMMARY OF THE INVENTION It is an object of the present invention to provide an improved dressing product which not only meets the requirements of conventional microenvironment for protection, isolation, and wound healing, but also promotes cell growth and shortens healing time.

In order to achieve the above object, the present invention provides an improved dressing product comprising: a matrix material usable for a dressing, and mussel mucin which is curable on the matrix material.

Mussel mucin used herein refers to Mytilus edulis Linnaeus, Mytilus coruscus or Perna viridis from the Mytilidae bivalve mollusc. 11 subclasses of mussel mucin, currently known as purified from marine mussels: mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen pre-COL- P, A mixture of one or more of pre-COL-D, pre-COL-NG, foot silk matrix protein PTMP and DTMP. The mussel viscosity of the mussel mucin used herein may have a pH of 1.0 to 7.0 in an aqueous solution, and particularly may be in the range of pH 3.0 to 6.5 to make the effect better.

The mussel mucin used herein may also be obtained by a method of biosynthesis, comprising a mixture of one or more of the known 11 mussel mucin subclasses. The artificially biosynthesized mussel mucin used herein may have a pH of 1.0 to 7.0 in aqueous solution, and particularly may be in the range of pH 3.0 to 6.5 to make it more effective.

The mussel mucin used herein may also be a hydrolyzed peptide obtained by hydrolysis of mussel mucin from natural or artificial biosynthesis, or a synthetic peptide containing a functional group thereof obtained by artificial synthesis. The mussel mucin hydrolyzed peptide or synthetic peptide used herein may have a pH of 1.0 to 7.0 in an aqueous solution, and particularly may be in the range of pH 3.0 to 6.5 to make the effect better.

The mussel mucin used herein can be obtained by the following preparation method, for example, a method for separating and purifying mussel mucin using mixed adsorption chromatography in Chinese Patent No. ZL200710179491.0, a kind of carboxy using Chinese Patent No. ZL200710179492.5 A method for purifying mussel mucin by methyl ion exchange chromatography, a method for separating and purifying mussel mucin using salting out and dialysis, Chinese Patent No. ZL200910087567.6.

The mussel mucin used herein may be in the form of a solution or a lyophilized powder.

In an embodiment of the invention, the dressing may be artificial skin, pig skin dressing, hydrogel dressing, hydrocolloid dressing, sponge and non-woven dressing, fabric dressing, hemostatic dressing, anti-adhesive dressing, alginate dressing, activated carbon dressing , negative pressure drainage dressings, etc.

The invention provides a method for preparing an improved dressing comprising the steps of:

(1) preparing a mussel mucin solution,

(2) Curing the mussel mucin solution to the dressing matrix material to obtain an improved dressing.

The term "curing" as used in the present invention refers to the adsorption of mussel mucin to a dressing by physical means.

The present invention also provides an improved artificial skin in which mussel mucin is cured onto artificial skin that can be used as a dressing.

The present invention also provides an improved pigskin dressing in which mussel mucin is cured to a pigskin dressing useful as a dressing.

The present invention also provides an improved hydrogel dressing in which mussel mucin is cured to be useful as Dressing on the hydrogel.

The present invention also provides an improved hydrocolloid dressing in which mussel mucin is cured onto a hydrocolloid that can be used as a dressing.

The present invention also provides an improved sponge and nonwoven dressing in which mussel mucin is cured onto sponges and nonwoven dressings useful as dressings.

The present invention also provides an improved fabric dressing in which mussel mucin is cured onto a fabric that can be used as a dressing. The fabric dressing may include: a gauze dressing, a wound patch, a non-woven dressing, a belly towel, and the like.

The present invention also provides an improved hemostatic dressing in which mussel mucin is cured onto a hemostatic dressing useful as a dressing. The hemostatic dressing may include the following materials: collagen, gelatin, polysaccharides, chitosan, hemostatic gauze, cellulose, and the like.

The present invention also provides an improved anti-adhesion dressing in which mussel mucin is cured to an anti-adhesion dressing useful as a dressing. The anti-adhesion dressing may include the following materials: sodium hyaluronate, polylactic acid, poloxamer, chitosan, polyethylene glycol, chitosan, and the like.

The present invention also provides an improved alginate dressing in which mussel mucin is cured onto an alginate dressing useful as a dressing.

The present invention also provides an improved activated carbon dressing in which mussel mucin is cured onto an activated carbon dressing useful as a dressing.

The present invention also provides an improved negative pressure drainage dressing in which mussel mucin is cured onto a negative pressure drainage dressing that can be used as a dressing.

In some embodiments, the mussel mucin solution can be added to the matrix material and the mussel mucin is cured to the matrix material after a period of time.

In some embodiments, the mussel mucin solution can be sprayed onto the surface of the matrix material for a period of time to cure the mussel mucin to the matrix material.

In some embodiments, the matrix material can be infiltrated in the mussel mucin solution for a period of time to cure the mussel mucin to the matrix material.

In an embodiment of the invention, the mussel mucin solution may be formulated from an acidic, neutral or weakly alkaline solution and is most preferably an acidic solution.

In the embodiment of the present invention, the mussel mucin solution may be formulated from citric acid, acetic acid, sodium citrate, oxalic acid, carbonic acid, phosphoric acid, benzoic acid, sodium carbonate, or the like, but is not limited to the above solvent.

In an embodiment of the present invention, the concentration of the mussel mucin solution may be 0.01-150. The concentration of mg/ml, in particular mussel mucin solution, may be from 0.1 to 15 mg/ml.

In an embodiment of the invention, the mussel mucin solution is used in an amount sufficient to uniformly cover or infiltrate the dressing.

In an embodiment of the invention, the temperature for curing may be any, preferably 10-40 °C.

In an embodiment of the invention, the period of time for curing may be any, preferably from 10 to 60 minutes.

The present invention provides an improved dressing formed by coating mussel mucin on a matrix material which promotes cell growth and shortens healing time while protecting the wound.

The dressing definition described in the present invention is derived from the Catalogue of Medical Devices (2013 Edition) promulgated by the State Food and Drug Administration.

The present inventors have unexpectedly found that the mussel mucin is cured on the dressing, avoiding the problem of requiring multiple administration due to factors such as bleeding of the wound surface, and can exert the activity of mussel mucin on the dressing, and impart the biological structure of the dressing. For example, accelerating wound healing is more conducive to reconstruction or rehabilitation of damaged parts, and mussel mucin solidified dressing improves the physical and chemical stability of the protein, prolongs the degradation cycle of the protein, and is more conducive to mussel mucinous organisms. The play of activity.

detailed description

The invention will now be further described in conjunction with specific embodiments. It should be noted that the dressing product of the present invention having mussel mucin cured can be prepared by the above method, and the effects have been tested and verified within the scope of the present invention, hereinafter, merely for illustration, only in the examples. A few of them are described, but should not be construed as limiting the invention. The nature and advantages of the products of the present invention are further illustrated in the following non-limiting examples.

Example 1: Mussel mucin solidified onto polylactic acid membrane artificial skin to promote burn wound healing

Preparation of mussel mucin-cured artificial skin: Prepare 10ml of mussel mucin solution of 5mg/ml with citric acid of pH 5.0, and place polylactic acid artificial skin (Jiangsu Huayi Cell Tissue Engineering Co., Ltd., 100mm×100mm) in mussel The mucin solution was kept at room temperature for 2 hours, and taken out after use.

Five male patients with large-area third-degree burns were collected. The burn area was greater than 10% of the total surface area, and two doctors in the burn department confirmed that they belonged to the third-degree burn. Using the same body control, artificial skin cured with mussel mucin and artificial skin treated with mussel mucin solution before use were used.

The healing of the burn site was observed 24 days after surgery. The results showed that after treatment with mussel mucin cured artificial skin, 3 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, and 2 cases were not completely healed, but the healing rate reached 92.36%. The artificial skin treated with the mussel mucin solution before use was not completely healed, and the average healing rate was 80.21%. It can be seen that artificial skin that cures mussel mucin is significantly beneficial for promoting healing of burn wounds.

Example 2: Mussel mucin solidified to hyaluronic acid membrane artificial skin to promote burn wound healing

Muscle mucin-cured artificial skin preparation: Prepare 1ml of mussel mucin solution of 1.5mg/ml with acetic acid of pH 4.0, and spray evenly onto the inner side of hyaluronic acid membrane artificial skin (Shandong Freda Pharmaceutical Co., Ltd., 100mm×100mm) , 25 ° C incubator for 2 hours, take out and set aside.

Ten male patients with large-area third-degree burns were collected, and the burn area was greater than 10% of the total surface area. Two doctors in the burn department confirmed that they belonged to the third-degree burn. Using the same body control, artificial skin cured with mussel mucin and artificial skin treated with mussel mucin solution before use were used.

The healing of the burn site was observed 24 days after surgery. The results showed that after the artificial skin was cured by mussel mucin, 7 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, and 3 cases were not completely healed, and the healing rate reached 95.07%. The artificial skin treated with the mussel mucin solution before use was not completely healed, and the average healing rate was 83.44%. It can be seen that artificial skin that cures mussel mucin is significantly beneficial for promoting healing of burn wounds.

Example 3: Mussel mucin solidified to glutaraldehyde cross-linked pig skin dressing to promote burn wound healing

Mussel mucin solidified pig skin dressing preparation: Prepare 50mg of mussel mucin solution of 1.0mg/ml with acetic acid of pH 4.0, cross-link pig skin dressing of glutaraldehyde (Jiangyin Benxiang Biotechnology Co., Ltd., 200mm×100mm) Place in mussel mucin solution, keep at room temperature for 2 hours, remove and set aside.

Five male patients with large-area third-degree burns were collected. The burn area was greater than 10% of the total surface area, and two doctors in the burn department confirmed that they belonged to the third-degree burn. An allogeneic control was used, respectively, using mussel mucoid-cured pigskin dressings and pigskin dressings sprayed with mussel mucin solution prior to use.

The healing of the burn site was observed 24 days after surgery. The results showed that after treatment with mussel mucoid-cured pigskin dressing, 4 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, and 1 case was not completely healed, and the healing rate reached 91.98%. Pig skin dressings sprayed with mussel mucin solution before use were not completely healed after treatment, and the average healing rate was 81.72%. It can be seen that the pig skin dressing of mussel mucin is more effective in promoting the healing of burn wounds.

Example 4: Curing mussel mucin to irradiate pig skin dressing to promote burn wound healing

Mussel mucin solidified pig skin dressing preparation: Prepare 5ml of 0.50mg/ml mussel mucin solution with oxalic acid of pH 3.0, and spray evenly onto the inner side of irradiated pig skin dressing (Jiangyin Benxiang Biotechnology Co., Ltd., 200mm×100mm) The 25 ° C incubator was kept for 1 hour, and it was taken out after use.

Ten male patients with large-area third-degree burns were collected, and the burn area was greater than 10% of the total surface area. Two doctors in the burn department confirmed that they belonged to the third-degree burn. An allogeneic control was used, respectively, using mussel mucoid-cured pigskin dressings and pigskin dressings sprayed with mussel mucin solution prior to use.

The healing of the burn site was observed 24 days after surgery. The results showed that after treatment with mussel mucoid-cured pigskin dressing, 7 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, 3 cases were not completely healed, and the healing rate reached 85.90%. Pig skin dressings sprayed with mussel mucin solution before use were not completely healed, and the average healing rate was 72.41%. It can be seen that the pigskin dressing of the cured mussel mucin is significantly beneficial for promoting the healing of burn wounds.

Example 5: Curing mussel mucin to a hydrogel dressing to promote ulcer wound healing

Preparation of Mussel Mucin Curing Hydrogel Dressing: Prepare 5ml of mussel mucin solution of 10mg/ml with pH 1.0, and apply hydrogel dressing (Rosik Kang Medical Hydrogel Dressing, Huizhou Huayang Medical Instrument Co., Ltd.) , 250mm × 100mm) placed in mussel mucin solution, kept at 10 ° C incubator for 30 minutes, taken out and set aside.

Ten patients with ulcerative wounds were collected and confirmed by dermatologists. The patients were randomly assigned to the test group or the control group, using mussel mucin-cured hydrogel dressing and mussel mucin-coated hydrogel dressing. treatment.

The healing of ulcer wounds was observed 1 week after treatment. The results showed that after treatment with the mussel dressing cured by mussel mucin, 6 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, 4 cases were not completely healed, and the healing rate reached 90.63%. . The hydrogel dressings sprayed with mussel mucin solution before use were not completely healed, and the average healing rate was 81.16%. It can be seen that mussel mucin-cured hydrogel dressings are significantly beneficial in promoting healing of ulcer wounds.

Example 6: Curing mussel mucin to a hydrogel dressing to promote hemorrhoid wound healing

Mussel mucin cured hydrogel dressing preparation: Prepare 1ml of 0.3mg/ml mussel mucin solution with benzoic acid of pH 3.0, and spray evenly onto hydrogel dressing (Rosco Kang medical hydrogel dressing, Huizhou Huayang Medical Devices Co., Ltd., 250mm × 100mm) inside, 10 ° C incubator for 10 minutes, take out and set aside.

Eight patients with acne wounds were collected and confirmed by dermatologists. The patients were randomly assigned to the test group or the control group, respectively, using mussel mucin-cured hydrogel dressings and spraying mussel mucin solution before use. Hydrogel dressing treatment.

The healing of acne wounds was observed 3 weeks after treatment. The results showed that after treatment with the mussel dressing cured by mussel mucin, 3 cases were completely healed, showing no wound exudation, the base was covered by new epithelium, and 1 case was not completely healed, and the healing rate reached 92.39%. . One patient was completely healed after treatment with a hydrogel dressing that sprayed mussel mucin solution before use, with an average healing rate of 80.78%. It can be seen that mussel mucin-cured hydrogel dressings are significantly beneficial in promoting healing of acne wounds.

Example 7: Curing mussel mucin to a hydrocolloid dressing to promote wound healing

Mussel mucin solidified hydrocolloid dressing preparation: 100ml of mussel beet protein solution of 1mg/ml was prepared by taking carbonate of pH 6.0, and the hydrocolloid dressing (Kanglidi medical hydrocolloid dressing, Zhejiang Kanglidi Medical Products Co., Ltd., 250mm×100mm) was placed. In the mussel mucin solution, it was kept at room temperature for 45 minutes, taken out and set aside.

Ten patients with abrasions were collected and randomly assigned to the test group or the control group, respectively, using a hydrocolloid dressing cured with mussel mucin and a hydrocolloid dressing sprayed with mussel mucin solution before use.

The healing of the wounds was observed 1 week after treatment. The results showed that after treatment with the hydrocolloid dressing cured by mussel mucin, 3 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, and 2 cases were not completely healed, and the healing rate reached 96.23%. After treatment with the hydrocolloid dressing of the mussel mucin solution before use, 2 patients were completely healed, and the average healing rate was 90.01%. It can be seen that mussel mucin-cured hydrocolloid dressing is significantly beneficial for promoting healing of ulcer wounds.

Example 8: Curing mussel mucin to a PU foam dressing to promote wound healing

Preparation of Mussel Mucin Curing Foam Dressing: Prepare 100ml of mussel mucin solution of 0.01mg/ml with citric acid of pH 5.5, and apply PU foam dressing (Conwell's exudate absorbing paste, Danish Coloplast, 12cm×12cm) Place in mussel mucin solution, keep at room temperature for 60 minutes, remove and dry at 40 ° C for later use.

Ten patients with abrasions were collected and randomly assigned to the test group or the control group, respectively, using a mussel mucin-cured PU foam dressing and a PU foam dressing sprayed with mussel mucin solution before use.

The healing of the wounds was observed 1 week after treatment. The results showed that after treatment with PU foam dressing cured by mussel mucin, 4 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, and 1 case was not completely healed, and the healing rate reached 90.92%. After treatment with the PU foam dressing of the mussel mucin solution before use, one patient was completely healed, and the average healing rate was 81.27%. It can be seen that the mussel mucin-cured PU foam dressing is significantly beneficial for promoting healing of ulcer wounds.

Example 9: Mussel mucin is cured to a calcium alginate dressing to promote hemorrhoid wound healing

Mussel mucin solidified calcium alginate dressing preparation: Prepare 50ml of 0.1mg/ml mussel mucin solution with benzoic acid of pH 3.5, calcium alginate dressing (moisturizing calcium alginate wound dressing, German moisturizing company, 10cm ×20 cm) was placed in mussel mucin solution, kept at room temperature for 45 minutes, and taken out and dried at 40 ° C for use.

Eight patients with acne wounds were collected and confirmed by dermatologists. They were randomly assigned to the test group or the control group. The calcium alginate dressing cured with mussel mucin and the alginic acid sprayed with mussel mucin solution before use. Calcium dressing treatment.

The healing of acne wounds was observed 2 weeks after treatment. After treatment with calcium alginate dressing cured by mussel mucin, one patient completely healed, showing no wound exudation, the base was covered by new epithelium, and 3 cases were not completely healed, and the healing rate reached 93.22%. Calcium alginate dressings sprayed with mussel mucin solution before use were not completely healed, and the average healing rate was 84.17%. It can be seen that the mussel mucin cured calcium alginate dressing is significantly beneficial for promoting the healing of acne wounds.

Example 10: Mussel mucin is cured onto a silver ion dressing to promote ulcer wound healing

Preparation of mussel mucin solidified silver ion dressing: Prepare 20ml of mussel mucin solution of 10mg/ml with acetic acid of pH 6.0, silver ion dressing (silver ion wound antibacterial dressing, Hunan Anxin medical polymer Material Co., Ltd., 5.5 cm × 7.5 cm) was placed in a mussel mucin solution, kept at a constant temperature of 25 ° C for 45 minutes, and taken out for use.

Twelve patients with ulcerative wounds were enrolled and confirmed by the surgeon. The patients were randomly assigned to the test group or the control group. The silver ion dressings cured with mussel mucin and silver ions sprayed with mussel mucin solution before use. Dressing treatment.

The healing of ulcer wounds was observed 1 week after treatment. After treatment with the silver ion solidified by mussel mucin, 5 cases were completely healed, showing no wound exudation, the base was covered by the neonatal epithelium, and 1 case was not completely healed, and the healing rate reached 92.28%. After treatment with the silver ion dressing of the mussel mucin solution before use, one case was completely healed, and the average healing rate was 77.52%. It can be seen that mussel mucin-cured silver ion dressing is significantly beneficial for promoting healing of ulcer wounds.

Example 11: Mussel mucin is cured onto an interactive dressing to promote burn wound healing

Mussel mucin solidified interactive dressing preparation: 50ml of mussel mucin solution of 1mg/ml was prepared by taking citric acid of pH 3.0, and interactive dressing (Dewewei, Baoheman Co., Ltd., 10cm×10cm) was placed in 贻The shellfish protein solution was kept at a constant temperature of 25 ° C for 45 minutes, and taken out after use.

Five male patients with large-area third-degree burns were collected. The burn area was greater than 10% of the total surface area, and two doctors in the burn department confirmed that they belonged to the third-degree burn. An analog dressing was used with an interactive dressing cured with mussel mucin and an interactive dressing that sprayed mussel mucin solution prior to use.

The healing of the burn site was observed 24 days after surgery. After treatment with the mussel mucin solidified interactive dressing, 4 cases were completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, and 1 case was not completely healed, and the healing rate reached 91.78%. After treatment with the interactive dressing of mussel mucin before use, the patients were not completely healed, and the average healing rate was 80.04%. It can be seen that the interactive dressing of cured mussel mucin is significantly beneficial for promoting healing of burn wounds.

Example 12: Curing mussel mucin to oil yarn promotes wound healing of skin laceration

Preparation of mussel mucin cured oil yarn: Prepare 10ml of mussel mucin solution of 2mg/ml with acetic acid of pH 5.0, and place the oil yarn (Comwl water colloidal oil yarn, Kanglebao Co., Ltd., 10cm×10cm) in 贻The shellfish protein solution was kept at room temperature for 45 minutes, and taken out after use.

10 male patients with laceration were collected, the tear length was greater than 5 cm, and confirmed by 2 surgeons After enrollment, the patients were randomly assigned to the test group or the control group. After the wound was sutured, the mussel mucin-cured oil yarn and the oil yarn sprayed with the mussel mucin solution before use were treated.

The healing of the wound site was observed 24 days after surgery. After treatment with mussel mucin cured oil yarn, 4 cases were completely healed, showing no wound exudation, the base was covered by new epithelium, and 1 case was not completely healed, and the healing rate reached 91.36%. After the treatment with the oil gauze coated with mussel mucin, the patients were not completely healed, and the average healing rate was 80.21%. It can be seen that the oil yarn of the cured mussel mucin is significantly beneficial for promoting the healing of the wound wound.

Example 13: Mussel mucin is cured onto gauze to promote wound healing in animal bites

Preparation of mussel mucin cured gauze: Prepare 10ml of 2mg/ml mussel mucin solution with pH 6.0, spray evenly onto gauze (medical gauze, Henan Jianqi Medical Instrument Co., Ltd., 10cm×10em), constant temperature at 25°C Hold for 1 hour, take it out and set aside.

Twelve animal bites were collected and randomly assigned to the test group or the control group. The wounds were sutured with mussel mucin-cured gauze and gauze sprayed with mussel mucin solution before use.

The healing of the wound site was observed 14 days after surgery. After treatment with mussel mucin-cured gauze, 5 patients completely healed, showing no wound exudation, the base was covered by neoplastic epithelium, and 1 case was not completely healed, and the healing rate reached 95.32%. After treatment with patients with gauze sprayed with mussel mucin solution before use, 3 patients completely healed with an average healing rate of 85.28%. It can be seen that the gauze of the cured mussel mucin is significantly beneficial for promoting the healing of the bite wound of the animal.

Claims (21)

An improved dressing comprising mussel mucin and a dressing matrix material, wherein the mussel mucin is cured on the dressing matrix material in a uniform coverage. The improved dressing according to claim 1 wherein said mussel mucin is from a subclass: mefp1, mefp-2, mefp-3, mefp-4, mefp-5, mefp-6, collagen pre-COL-P a mixture of one or more of pre-COL-D, pre-COL-NG, foot silk matrix proteins PTMP and DTMP. The improved dressing of claim 1 wherein the dressing is selected from the group consisting of artificial skin, pig skin dressings, hydrogel dressings, hydrocolloid dressings, sponge and nonwoven dressings, fabric dressings, including gauze dressings, bandages, nonwoven dressings, Abdominal towel, hemostatic dressing, including hemostatic dressing containing collagen, gelatin, polysaccharide, chitosan, hemostatic gauze, cellulose, anti-adhesive dressing, including sodium hyaluronate, polylactic acid, poloxamer, chitin Sugar, polyethylene glycol, chitosan anti-adhesive dressing, alginate dressing, activated carbon dressing, negative pressure drainage dressing. A method of making an improved dressing comprising the steps of: (1) preparing a mussel mucin solution, (2) Curing the mussel mucin solution to the dressing matrix material to obtain an improved dressing. A method according to claim 4, wherein the curing is by adding the mussel mucin solution to the dressing matrix material, or spraying onto the surface of the dressing matrix material, or infiltrating the dressing matrix material into the mussel mucin solution for a period of time. The mucin is cured to the dressing matrix material. A method according to claim 4 wherein the mussel mucin solution is formulated from an acidic, neutral or weakly alkaline solution, especially from an acidic solution. The method of claim 6 wherein the mussel mucin solution is formulated from citric acid, acetic acid, sodium citrate, oxalic acid, carbonic acid, phosphoric acid, benzoic acid, sodium carbonate. A method according to any one of claims 4-7, wherein the mussel mucin concentration is from 0.01 to 150 mg/ml, in particular from 0.1 to 15 mg/ml. A method according to any one of claims 4 to 7, wherein the curing temperature is from 10 to 40 °C. A method according to any one of claims 4 to 7, wherein the curing time is from 10 to 60 minutes. An improved artificial skin in which mussel mucin is cured in a uniform coverage on artificial skin material. An improved pigskin dressing in which mussel mucin is cured in a uniform coverage on a pigskin dressing. An improved hydrogel dressing in which mussel mucin is cured in a uniform coverage on a hydrogel dressing. An improved hydrocolloid dressing in which mussel mucin is cured in a uniform coverage on a hydrocolloid dressing. An improved sponge and nonwoven dressing in which mussel mucin is cured in a uniform coverage on sponges and nonwoven dressings. An improved fabric dressing in which mussel mucin is cured in a uniform coverage on a fabric dressing. An improved hemostatic dressing in which mussel mucin is cured in a uniform coverage on a hemostatic dressing. An improved anti-adhesion dressing in which mussel mucin is cured in a uniform coverage on an anti-adhesion dressing. An improved alginate dressing in which mussel mucin is cured in a uniform coverage on an alginate dressing. An improved activated carbon dressing in which mussel mucin is cured in a uniform coverage on an activated carbon dressing. An improved negative pressure drainage dressing in which mussel mucin is cured in a uniform coverage on a negative pressure drainage dressing.