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WO2017199993A1 - Dispositif de séparation - Google Patents

Dispositif de séparation Download PDF

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Publication number
WO2017199993A1
WO2017199993A1 PCT/JP2017/018486 JP2017018486W WO2017199993A1 WO 2017199993 A1 WO2017199993 A1 WO 2017199993A1 JP 2017018486 W JP2017018486 W JP 2017018486W WO 2017199993 A1 WO2017199993 A1 WO 2017199993A1
Authority
WO
WIPO (PCT)
Prior art keywords
hole
separation
separation layer
separation device
diameter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2017/018486
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English (en)
Japanese (ja)
Inventor
悠司 豊田
山本　元
片山　博之
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Corp
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Nitto Denko Corp
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Filing date
Publication date
Application filed by Nitto Denko Corp filed Critical Nitto Denko Corp
Publication of WO2017199993A1 publication Critical patent/WO2017199993A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/06—Flat membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • the present invention relates to a separation device.
  • Patent Document 1 discloses a platelet production apparatus, and a megakaryocyte is held on a holding member by a holding member in which an opening such as a hole having a diameter that allows passage of platelets is formed. It is disclosed that platelets already produced from megakaryocytes and platelets produced from megakaryocytes are separated by passing through an opening.
  • an object of the present invention is to provide a separation device that can reliably separate a holding object and a separation object with excellent separation efficiency.
  • the present invention has a first surface and a second surface facing the first surface, and a structure including a through-hole penetrating between the first surface and the second surface;
  • a separation device comprising a third surface and a porous separation layer having a fourth surface facing the third surface,
  • the structure and the separation layer are laminated so that the second surface side of the structure and the third surface side of the separation layer face each other.
  • the hole diameter of the micropore in the said 3rd surface of the said separation layer is related with the separation device smaller than the hole diameter of the said through-hole in the said 2nd surface of the said structure.
  • the entire separation layer may be porous.
  • the plurality of micro holes in the third surface of the separation layer may be connected to the through hole in the second surface of the structure.
  • the structure and the separation layer may be directly laminated.
  • At least one of the structure and the separation layer may be formed of a polymer material.
  • the separation layer may include a plurality of micropores penetrating between the third surface and the fourth surface.
  • the diameter of the through hole on the first surface side may be larger than the diameter of the through hole on the second surface side.
  • the diameter of the through hole on the first surface side may be smaller than the diameter of the through hole on the second surface side.
  • a separation device is a separation device that separates a holding object and a separation object, and the micropores do not pass the holding object and the separation object passes. It may have a possible pore size.
  • the holding object may be held by the through hole.
  • a separation device capable of reliably separating a holding object and a separation object with excellent separation efficiency is provided.
  • FIG. 1 (a) to 1 (c) are schematic cross-sectional views showing several embodiments of the separation device of the present invention.
  • FIG. 2 shows the amount of particles remaining after dripping and washing (unit: mg) when the aqueous dispersion containing polystyrene particles 1 was dripped and washed using the separation devices (filters) of each Example and Comparative Example. It is a graph showing the ratio (weight%) with respect to the particle amount (5 mg) before a process.
  • FIG. 3 shows the amount of particles remaining after dripping and washing (unit: mg) when the aqueous dispersion containing polystyrene particles 2 was dripped and washed using the separation devices (filters) of each Example and Comparative Example.
  • FIG. 4 shows the amount of particles remaining after dropping and washing (unit: mg) when the aqueous dispersion containing polystyrene particles 3 was dropped and washed using the separation devices (filters) of each Example and Comparative Example. It is a graph showing the ratio (weight%) with respect to the particle amount (5 mg) before a process.
  • the retention target is not particularly limited, but a cell is exemplified, and the separation target is not particularly limited, but from a cell differentiated from a cell as a retention target or a cell as a retention target. Examples include metabolized metabolites and the like.
  • the separation device of the present embodiment from a culture solution containing megakaryocytes and platelets already produced from megakaryocytes, produced from platelets and megakaryocytes already produced from megakaryocytes Use as a separation device for regenerative medicine, such as separating platelets to be used.
  • the separation device 1 of the embodiment shown in FIGS. 1A to 1C has a first surface 101 and a second surface 102 facing the first surface 101, and the first surface 101 and the second surface And a porous separation layer 21 having a structure 11 having a through-hole 111 penetrating between them and a third surface 203 and a fourth surface 204 facing the third surface 203.
  • the structure 11 and the separation layer 21 are laminated so that the second surface 102 side of the structure 11 and the third surface 203 side of the separation layer 21 face each other.
  • the hole diameter of the minute hole 211 on the third surface 203 of the separation layer 21 is smaller than the hole diameter of the through hole 111 on the second surface 102 of the structure 11.
  • the separation device 1 of the present embodiment reduces the pressure on the fourth surface 204 side than the first surface 101 side, so that the holding object and the holding object are mixed and / or separated from the holding object.
  • the object to be held is mixed with the object to be held while being held by the through hole 111 without passing through the micro holes 211 and / or
  • the separation object separated from the holding object is used so as to be separated by passing through the micro holes 211.
  • the medium is appropriately selected according to the type and properties of the holding object and the separation object, but is typically liquid or gas. For example, when separating platelets already produced from megakaryocytes and platelets produced from megakaryocytes from a culture solution containing megakaryocytes and platelets already produced from megakaryocytes, the culture solution is the medium.
  • the structure 11 has a first surface 101 and a second surface 102 that faces the first surface 101, and penetrates between the first surface 101 and the second surface 102.
  • a through-hole 111 is provided.
  • the through hole 111 of the structure 11 plays a role of holding the object to be held in the vicinity of the minute hole 211 of the separation layer 21.
  • the structure 11 preferably has a plurality of through holes 111.
  • the cross-sectional shape of the through-hole 111 in the thickness direction of the structure 11 is a through-hole penetrating between the first surface 101 and the second surface 102 of the structure 11.
  • a shape hereinafter referred to as a straight hole
  • the diameter of the through hole 111 is the same from the first surface 101 side to the second surface 102 side.
  • a shape in which the hole diameter of the through hole 111 decreases from the first surface 101 side to the second surface 102 side (hereinafter also referred to as a taper shape).
  • part of the cross-sectional shape includes a reverse taper shape, such as a combination of a straight shape and a taper shape. It may have a shape such as a partially reverse tapered shape.
  • the cross-sectional shape of the through-hole 111 of the structure 11 is that the object to be held that has once entered the through-hole 111 is easily held in the through-hole 111.
  • a shape in which the hole diameter of the through hole 111 in the first surface 101 is smaller than the hole diameter of the through hole 111 in the second surface 102 is preferable, such as a tapered shape or a partially reverse tapered shape.
  • the hole diameter of the through hole 111 on the first surface is larger than the hole diameter of the through hole 111 on the second surface 102 such as a tapered shape or a partially tapered shape. Shape is also preferred.
  • the hole diameter of the through hole 111 is not particularly limited as long as it is a hole diameter capable of holding the holding object in the through hole 111, and is appropriately determined in consideration of the shape of the through hole 111, the size of the holding object, and the like. Just choose.
  • the hole diameter of the through-hole 111 is, for example, FIG. ) Is preferably 20 to 500 ⁇ m, more preferably 50 to 200 ⁇ m. In the case of the tapered shape shown in FIG.
  • the hole diameter of the through hole 111 on the first surface 101 side is preferably 50 to 1000 ⁇ m, and more preferably 100 to 500 ⁇ m.
  • the diameter of the through hole 111 on the second surface 102 side is preferably 10 to 500 ⁇ m, more preferably 20 to 250 ⁇ m.
  • the hole diameter of the through hole 111 on the first surface 101 side is preferably 20 to 1000 ⁇ m, and more preferably 50 to 500 ⁇ m.
  • the hole diameter of the through hole 111 on the second surface 102 side is preferably 50 to 5000 ⁇ m, and more preferably 100 to 1000 ⁇ m.
  • the hole diameter of the through-hole 111 can be measured with SEM, an optical microscope, etc.
  • planar shape of the through hole 111 on the first surface 101 side and the second surface 102 side is not particularly limited, and various shapes such as an ellipse, a triangle, a polygon such as a quadrangle, and the like can be used. Possible. Note that the planar shapes of the plurality of through holes 111 may be the same or different. Moreover, the planar shape of the first surface 101 side and the second surface 102 side of a certain through-hole 111 may be the same or different. In addition, when the planar shape of the through hole 111 is a shape other than a circle, the hole area is obtained, and the hole diameter (equivalent circle diameter) in a circle having the same hole area is regarded as the hole diameter of the through hole 111. To do.
  • the “tapered shape” in the present specification is not limited to the case where the planar shape of the through hole 111 is circular, and the thickness direction of the structure 11 even when the planar shape of the through hole 111 is other than circular. In this cross section, any shape in which the hole diameter decreases from the first surface 101 side to the second surface 102 side is included in this.
  • the “reverse taper shape” is also interpreted in the same manner.
  • the hole density on the surface (the first surface 101 and the second surface 102) of the structure 11 is not particularly limited, but the hole density of the through holes 111 in the structure 11 is not limited. If it is 100 pieces / cm 2 or more, separation efficiency is improved by parallel processing, which is preferable. More preferably, it is 1000 pieces / cm ⁇ 2 > or more, More preferably, it is 10,000 pieces / cm ⁇ 2 > or more. On the other hand, it is preferable that the pore density is 100000 pieces / cm 2 or less because the porosity is not excessively increased and a decrease in strength can be prevented. More preferably, it is 50000 piece / cm ⁇ 2 > or less, More preferably, it is 10,000 piece / cm ⁇ 2 > or less.
  • the thickness of the structure 11 is not particularly limited as long as the object to be held is held in the through-hole 111, and may be appropriately selected in consideration of the size of the object to be held.
  • the thickness is preferably 10 to 1000 ⁇ m, more preferably 50 to 300 ⁇ m.
  • an organic material such as a polymer material or an inorganic material such as silicon or metal can be used.
  • non-reactive polymer materials and biocompatible metal materials are used because they do not exhibit cytotoxicity. Etc. are preferred.
  • the second surface 102 side of the structure 11 is laminated so that the third surface 203 side of the porous separation layer 21 having a plurality of micropores 211 faces each other.
  • the hole diameter of the micro hole 211 in the third surface 203 of the separation layer 21 is smaller than the hole diameter of the through hole 111 in the second surface 102 of the structure 11.
  • the object to be held is held in the vicinity of the minute hole 211 of the separation layer 21 by the through hole 111 of the structure 11 without passing through the minute hole 211 of the separation layer 21.
  • the separation object mixed with and / or separated from the retention object passes through the micro holes 211 from the third surface 203 side of the separation layer 21 to the fourth surface 204 side of the separation layer 21. Separated.
  • the through hole 111 of the structure 11 is surely provided in the vicinity of the micro hole 211 of the separation layer 21 without passing the object to be held through the micro hole 211 of the separation layer 21.
  • the separation target mixed with the separation target and / or separated from the retention target can be efficiently separated through the micro holes 211 of the separation layer 21. Therefore, for example, when separating a cell as a retention target from a separation target such as a cell differentiated from a cell as a retention target or a metabolite metabolized from a cell as a retention target, it is excellent. Separation can be reliably performed with separation efficiency. Further, since the structure 11 and the separation layer 21 have a laminated structure, the separation device can be used even when many through holes 111 are provided in the structure 11 in order to efficiently hold the object to be held. The necessary strength can be secured.
  • the hole diameter of the minute hole 211 of the separation layer 21 is not particularly limited as long as it is smaller than the hole diameter of the through hole 111 in the second surface 102 of the structure 11, and the size of the holding object, the size of the separation object, and the like. It may be selected as appropriate in consideration, but in order to perform reliable separation, it is preferable that the hole diameter is such that the object to be held is not allowed to pass through and the object to be separated can pass. According to this viewpoint, it is preferable that the hole diameter of the micropore is smaller than the size of the holding object and larger than the size of the separation object.
  • the size of the megakaryocytes that are the retention target is usually about 20 to 150 ⁇ m, and the size of the platelets that are the separation target is about 2 to 5 ⁇ m.
  • the pore diameter is preferably 2 to 15 ⁇ m, more preferably 3 to 6 ⁇ m.
  • the hole diameter of a micropore can be measured with SEM, an optical microscope, etc.
  • micro holes 211 in the third surface 203 of the separation layer 21 may be connected to one through hole 111 in the second surface 102 of the structure 11, but the separation efficiency is further improved.
  • a plurality of micro holes 211 in the third surface 203 of the separation layer 21 are connected to one through hole 111 in the second surface 102 of the structure 11.
  • the separation device 1 having such a configuration even if a part of the plurality of micro holes 211 connected to the through hole 111 is clogged, the micro hole that is not clogged is formed. This is also preferable because the separation function can be ensured by 211.
  • the number of micropores 211 to be formed is preferably as many as possible, but is not particularly limited, and is, for example, 2 to 1 million, and preferably 3 to 1 million.
  • the average pore diameter of the plurality of micropores 211 may be appropriately selected in consideration of the size of the object to be held, the size of the object to be separated, and the like.
  • the average pore diameter is 2 to 15 ⁇ m.
  • the thickness is preferably 0.1 to 2.0 ⁇ m, more preferably 0.2 to 1.0 ⁇ m.
  • the cross-sectional shape of the micropores 211 in the porous separation layer 21 in the thickness direction of the separation layer 21 allows the separation target to pass from the third surface 203 side to the fourth surface 204 side.
  • the shape is not particularly limited, but may be a plurality of micro holes penetrating the separation layer 21, for example, a straight hole shape as shown in FIGS. 1 (a) to 1 (c). It may have a shape, or a shape such as a partially tapered shape or a partially inverted tapered shape. Or the random hole which a nonwoven fabric, Nitto Denko Co., Ltd. TEMISH (trademark), etc. have may be sufficient.
  • the cross-sectional shape and hole diameter of the plurality of micro holes 211 may be the same or different.
  • the shape of the micropore 211 in the separation layer 21 is a cross-section in the thickness direction of the separation layer 21.
  • the hole diameter of the minute hole 211 decreases from the third surface 203 side to the fourth surface 204 side (taper shape).
  • the pore diameter of the micropores 211 on the third surface 203 side is preferably 10 ⁇ m or more, more preferably 20 ⁇ m or more, and the micropores on the fourth surface 204 side.
  • the hole diameter of the hole 211 is preferably 10 ⁇ m or less, and more preferably 5 ⁇ m or less.
  • Pore density in the surface of the porous separation layer 21 having a plurality of micropores 211 is not particularly limited, 10 4 pore density of micropores 211 in the separation layer 21 is / Cm 2 or more is preferable because separation efficiency is improved by parallel processing. More preferably, it is 10 5 pieces / cm 2 or more, and further preferably 10 6 pieces / cm 2 or more. On the other hand, it is preferable that the pore density is 10 8 pieces / cm 2 or less because the porosity is not excessively increased and strength reduction can be prevented. More preferably, it is 10 7 pieces / cm 2 or less, and still more preferably 10 6 pieces / cm 2 .
  • the thickness of the separation layer 21 is not particularly limited, and may be appropriately selected in consideration of the size of the separation object.
  • the thickness is preferably 5 to 100 ⁇ m and more preferably 10 to 50 ⁇ m from the viewpoint of prevention of clogging and sheet strength.
  • an organic material such as a polymer material or an inorganic material such as silicon or metal can be used.
  • a nonwoven material and porous materials such as Nitto Denko Corporation TEMISH (trademark).
  • non-reactive polymer materials and biocompatible metal materials are used because they do not exhibit cytotoxicity. Etc. are preferred.
  • the structure 11 and the separation layer 21 may be directly laminated, or may be indirectly laminated using an adhesive or the like. Note that the state in which the structure 11 and the separation layer 21 are directly stacked means that no other layer is interposed between the structure 11 and the separation layer 21, and the second surface 102 of the structure 11 and the separation layer 21 are not present. This shows that the third surface 203 is in direct contact.
  • At least one of the structure 11 and the separation layer 21 is preferably formed of a flexible material such as a polymer material. If at least one of the structure 11 and the separation layer 21 is formed of a flexible material such as a polymer material, the adsorbing effect using the suction force from the micropores 211 can be used without using an adhesive or the like.
  • the structure 11 and the separation layer 21 can be directly adhered with high adhesion.
  • a gel material such as silicone resin or gelatin is particularly preferable because it has excellent wettability and can further enhance the adsorption effect.
  • the micro holes 211 in the separation layer 21 are formed at positions where the micro holes 211 in the third surface 203 of the separation layer 21 are connected to at least the through holes 111 in the second surface 102 of the structure 11.
  • the micropores 211 are preferably formed on the entire surface of the separation layer 21. That is, the entire separation layer 21 is preferably porous.
  • the separation device 1 having such a configuration even when a deviation occurs between the structure 11 and the separation layer 21 during use, the state in which the micro holes 211 are connected to each through-hole 111 is obtained. Since it can ensure, the separation function of the separation device 1 can be continuously secured. Such a configuration is particularly useful when the structure 11 and the separation layer 21 are directly laminated. Further, the separation layer 21 which is entirely porous is advantageous in terms of productivity.
  • both the structure 11 and the separation layer 21 are formed of a polymer material.
  • the separation device 1 of the present embodiment only needs to include at least the structure 11 and the separation layer 21, but may further include other layers such as a surface coating agent layer according to desired characteristics. Good. Further, the separation device 1 of the present embodiment may include a plurality of at least one of the structure 11 and the separation layer 21.
  • the manufacturing process of the structure 11 which comprises the separation device 1 of this invention is demonstrated.
  • a polymer material for example, known molding methods such as compression molding, injection molding, and hot roll transfer can be appropriately applied.
  • a semi-cured polymer material is molded by pressure using a mold corresponding to the shape of the through-hole of the target structure, and heated to allow the curing to proceed completely.
  • the structure 11 having the through hole 111 having a shape can also be manufactured by laser drilling to a polymeric material.
  • molding method and manufacturing method can be applied suitably.
  • a well-known manufacturing method is suitably applied as a manufacturing method of a porous material according to the kind of material which comprises the separation layer 21, and the hole diameter of the micropore 211. it can.
  • a resin film having a plurality of through-holes described in JP-A-2015-164728 is produced. A method or the like can be appropriately employed.
  • TEMISH registered trademark
  • the separation device 1 of this embodiment can be manufactured by laminating the structure 11 and the separation layer 21 thus obtained directly or indirectly via an adhesive or the like.
  • Comparative Example 1 Filter paper for Kiriyama funnel with a reserved particle diameter of 4 ⁇ m No. 5A (manufactured by Kiriyama Seisakusho Co., Ltd.) was used as the separation device (filter) of Comparative Example 1.
  • Comparative Example 2 A separation layer A (Osydisk manufactured by Oxyphen) having a thickness of 3.0 ⁇ m formed with a pore having a diameter of 3.0 ⁇ m formed on a non-porous base sheet made of PET by ion beam irradiation and etching was separated into a separation layer. Only as a separation device (filter) of Comparative Example 2.
  • a microphotograph of one surface of the separation membrane A was taken, and the number of holes per fixed area was counted to measure the micropore density of the separation membrane A at 10 locations.
  • the density of the through holes was 3.0 ⁇ 10 3 to It was 5.0 ⁇ 10 3 pieces / mm 2 .
  • Example 1 The separation membrane A used in Comparative Example 2 was used as the separation layer 21.
  • a taper-shaped through-hole sheet B having a 25 mm square and a thickness of 400 ⁇ m was produced by heat press molding using a semi-cured silicone resin sheet as the structure 11 and a mold having a plurality of convex shapes.
  • the semi-cured silicone resin sheet was prepared by mixing 99.0 wt% of methylated silicone (manufactured by Nitto Denko, KL-100) with 1.0 wt% of dimethylsilylated silica (manufactured by Nippon Aerosil Co., Ltd., AEROSIL R976S). It was obtained by coating to a thickness of 300 ⁇ m using a coating apparatus (PI-1210, manufactured by Tester Sangyo) and heating at 130 ° C. for 10 minutes with a dryer.
  • a coating apparatus PI-1210, manufactured by Tester Sangyo
  • the tapered through-hole sheet B is installed so that the surface side of the through-hole having a 250 ⁇ m square is laminated on the separation membrane A, and a laminated body is produced.
  • about 200,000 fine holes of the separation membrane A are connected to one 250 ⁇ m square through hole of the tapered through hole sheet B.
  • Example 2 As shown in FIG. 1C, a laminated body was produced in the same manner as in Example 1 except that the tapered through-hole sheet B was placed so that the surface side with the through-hole of 500 ⁇ m square was laminated on the separation membrane A. This was used as the separation device (filter) of Example 2. Here, about 750,000 micropores of the separation membrane A are connected to one 500 ⁇ m square through hole of the tapered through hole sheet B.
  • Example 3 As a separation membrane A, a separation device was produced by performing the same operation as in Example 1 except that a separation membrane having a diameter of through-holes of 1.0 ⁇ m was used.
  • Example 4 As a separation membrane A, a separation device was manufactured by performing the same operation as in Example 1 except that a separation membrane having a diameter of through-holes of 0.5 ⁇ m was used.
  • Test 1 Polystyrene particles 1 having an average particle size of 2.0 ⁇ m (CV value 5%, 2.5 wt%) (Polybeads manufactured by Polysciences), polystyrene having an average particle size of 6.0 ⁇ m (CV value 10%, 2.5 wt%) Particles 2 (manufactured by Polysciences, polybead), and polystyrene particles 3 in which polystyrene particles 1 and polystyrene particles 2 are mixed at a weight ratio of 1: 1, each diluted 10 times with ion-exchanged water, are used. The particle passage ability and retention ability of the filter were evaluated.
  • Each filter is set on a Kiriyama funnel, and 2 ml of polystyrene particle aqueous dispersion (0.25 wt%, particle amount 5 mg) is dropped while sucking from below, and particles remaining on and in the filter due to weight increase after dropping. The particle passing ability was confirmed by measuring the amount.
  • the filters of Examples 1 and 2 were set on the Kiriyama funnel so that the tapered through-hole sheet B was on the upper side and the separation membrane A was on the lower side (Kiriyama funnel side).
  • each filter carrying the remaining particles was set on a Kiriyama funnel, washed with 100 ml of ion-exchanged water while being sucked from below, and the particle retention ability was measured from the change in weight after washing.
  • Tables 1 and 2 show the results of evaluating the particle passage ability and retention ability for each of the polystyrene particles 1 to 3 for the filters of Examples and Comparative Examples in terms of the amount of remaining particles (unit: mg). These are the results of evaluating these by the ratio (% by weight) to the amount of particles (5 mg) before treatment.
  • Table 2 are summarized in the graphs of FIGS. In Tables 1 and 2 and FIGS. 2 to 4, the column “2 ⁇ m” is the evaluation result when polystyrene particles 1 are used, and the column “6 ⁇ m” is the evaluation result when polystyrene particles 2 are used. The column of “2 + 6 ⁇ m” is an evaluation result when polystyrene particles 3 are used.
  • the separation devices (filters) of Example 1 and Example 2 having both the separation membrane A and the tapered through-hole sheet B are based on the separation membrane A having micropores.
  • the tapered through-hole sheet B having a high separation performance and having a through-hole laminated on the upper part of the separation membrane A can keep many particles after washing.
  • Each filter is set on a Kiriyama funnel, and 2 ml of polystyrene particle aqueous dispersion (0.25 wt%, particle amount 5 mg) is dropped while sucking from below, and particles remaining on and in the filter due to weight increase after dropping. The particle passing ability was confirmed by measuring the amount.
  • the filters of Examples 3 and 4 were set on the Kiriyama funnel so that the tapered through-hole sheet B was on the upper side and the separation membrane A was on the lower side (Kiriyama funnel side). The results are shown in Table 3. As shown in Table 3, the separation devices (filters) of Example 3 and Example 4 having both the separation membrane A and the tapered through-hole sheet B have high separation performance due to the separation membrane A having micropores. all right.

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Abstract

La présente invention concerne un dispositif de séparation qui est pourvu : d'une structure qui possède une première surface et une deuxième surface faisant face à la première surface, ainsi que des trous traversants qui pénètrent entre la première surface et la deuxième surface ; d'une couche de séparation poreuse qui présente une troisième surface et une quatrième surface faisant face à la troisième surface, la structure et la couche de séparation étant stratifiées l'une sur l'autre de telle manière que le deuxième côté surface de la structure et le troisième côté surface de la couche de séparation se font face, et la taille des pores de chacun des micropores dans la troisième surface de la couche de séparation est inférieure à la taille des pores de chacun des trous traversants dans la deuxième surface de la structure. Selon ce dispositif de séparation, la séparation d'une substance à séparer d'une substance à garder est assurée avec une efficacité de séparation supérieure.
PCT/JP2017/018486 2016-05-17 2017-05-17 Dispositif de séparation Ceased WO2017199993A1 (fr)

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JP2016098854 2016-05-17

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115212931A (zh) * 2021-04-15 2022-10-21 武汉介观生物科技有限责任公司 一种血小板体外释放系统及血小板生产方法

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