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WO2017143355A2 - Tête de fabrication de cellules/substances biologiques à entraînement par vis direct destinée à l'assemblage de constructions de tissu en 3d - Google Patents

Tête de fabrication de cellules/substances biologiques à entraînement par vis direct destinée à l'assemblage de constructions de tissu en 3d Download PDF

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Publication number
WO2017143355A2
WO2017143355A2 PCT/US2017/021265 US2017021265W WO2017143355A2 WO 2017143355 A2 WO2017143355 A2 WO 2017143355A2 US 2017021265 W US2017021265 W US 2017021265W WO 2017143355 A2 WO2017143355 A2 WO 2017143355A2
Authority
WO
WIPO (PCT)
Prior art keywords
filament
bioactive
extruded
extruded bioactive
fabrication
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2017/021265
Other languages
English (en)
Other versions
WO2017143355A3 (fr
Inventor
Wei Sun
Qudus Hamid
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sunp Biotech LLC
Original Assignee
Sunp Biotech LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sunp Biotech LLC filed Critical Sunp Biotech LLC
Publication of WO2017143355A2 publication Critical patent/WO2017143355A2/fr
Publication of WO2017143355A3 publication Critical patent/WO2017143355A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Definitions

  • Tissue generation have advanced tremendously in recent decades. Precision motion systems present a unique opportunity to establish new platforms to fabrication living tissue constructs. The key of successfully building a functional tissue construct, is having the right tool. In order to assemble cells into a functional array, the fabrication head must sustain cell life and have full control of the fluid/bio-suspension being printed. This article presents a direct screw-driven cell/biologics deposition fabrication head for the assembly of three-dimensional tissue constructs. This fabrication head is designed to be 100% biocompatible, sustain cell life, and has full control of mass flow rate with interchangeable nozzles.
  • Figure 1 depicts the fabrication head with all its components labelled accordingly;
  • Figure 2 depicts the direct screw-driven plunger mechanism;
  • Figure 3 depicts the assembled deposition nozzle with all its components
  • Figure 4 depicts the motor and support bracket.
  • Figure 5 depicts the fabrication nozzle outlined in red mounted on a 3D motion system.
  • This fabrication head ( Figure 1) is specifically designed to print living cells and/or biologies. This head features precise control of the suspension within the deposition chamber.
  • the direct screw allows for uniform force distribution. This significantly reduces the shear forces within the deposition chamber and increases cell viability. Additionally, this mechanism provides relatively high applied force which allows for the deposition of an extensive library of biomaterials. Coupled with advanced motion controllers, this fabrication head can instantaneous change its driving direction, relative velocity, and applied force.
  • the motion/mass flow control eliminates any back-pressures that can be created with high viscosity materials. It also provides a start-stop mechanism that is crucial for multi-nozzle printing.
  • the assembly and mechanism of this direct screw-driven component is shown in Figure 2.
  • Multi -nozzle printing is the utilization of two or more fabrication head with two or more cells and/or biologies. These nozzles will function collectively with each other to print the
  • Cell viability is the most important aspect of cell printing. If cells are not alive after the fabrication process, it would be impossible to print functional tissue constructs. This fabrication head was specifically developed to maintain cell life and reduce or eliminate any and all mechanical force that may induce cell differentiate. Hence, at the end of the printing process, this print head will print viable, undifferentiated cells. Of course, cell viability starts with biocompatibility.
  • the entire fabrication head is biocompatible, being fabricated from FDA approved metals and/or plastics.
  • Figure 3 shows an image of the assembled deposition nozzle with all its components while Figure 4 is an image of the print heads' motor and support bracket.
  • FIG. 5 shows an image of the fabrication nozzle mounted on a 3D motion system that uses layer- by-layer fabrication technique to build tissue constructs.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)

Abstract

La génération de tissu a réalisé des progrès considérables ces dernières décennies. Les systèmes de mouvement de précision présentent une opportunité unique pour établir de nouvelles plateformes en vue de fabriquer des constructions de tissu vivant. La clé pour réussir l'élaboration d'une construction de tissu fonctionnelle est d'avoir le bon outil. Afin d'assembler des cellules pour former un réseau fonctionnel, la tête de fabrication doit maintenir les cellules en vie et doit avoir une maîtrise totale sur le fluide/la biosuspension à imprimer. La présente invention concerne une tête de fabrication de dépôt de cellules/substances biologiques à entraînement par vis direct destinée à l'assemblage de constructions de tissu tridimensionnelles. Ladite tête de fabrication est conçue pour être biocompatible à 100 % et pour maintenir les cellules en vie, et a une maîtrise totale du débit massique à l'aide de buses interchangeables.
PCT/US2017/021265 2016-01-20 2017-03-08 Tête de fabrication de cellules/substances biologiques à entraînement par vis direct destinée à l'assemblage de constructions de tissu en 3d Ceased WO2017143355A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662280775P 2016-01-20 2016-01-20
US62/280,775 2016-01-20

Publications (2)

Publication Number Publication Date
WO2017143355A2 true WO2017143355A2 (fr) 2017-08-24
WO2017143355A3 WO2017143355A3 (fr) 2017-11-09

Family

ID=59626332

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/021265 Ceased WO2017143355A2 (fr) 2016-01-20 2017-03-08 Tête de fabrication de cellules/substances biologiques à entraînement par vis direct destinée à l'assemblage de constructions de tissu en 3d

Country Status (1)

Country Link
WO (1) WO2017143355A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025083309A1 (fr) * 2023-10-17 2025-04-24 Administración General De La Comunidad Autónoma De Euskadi Dispositif d'extrusion intracorporel pour l'application de bio-encres, d'hydrogels ou de fluides en chirurgie laparoscopique

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005042623A1 (fr) * 2003-10-23 2005-05-12 University Of Nottingham Elaboration d'extrudats de polymeres actifs
US20110136162A1 (en) * 2009-08-31 2011-06-09 Drexel University Compositions and Methods for Functionalized Patterning of Tissue Engineering Substrates Including Bioprinting Cell-Laden Constructs for Multicompartment Tissue Chambers
US10538864B2 (en) * 2012-10-24 2020-01-21 Dsm Ip Assets, B.V. Fibers comprising polyesteramide copolymers for drug delivery
US20160000886A1 (en) * 2013-02-22 2016-01-07 President And Fellows Of Harvard College Nanostructured active therapeutic vehicles and uses thereof
WO2015077262A1 (fr) * 2013-11-19 2015-05-28 Guill Tool & Engineering Entrées d'impression 3d coextrudées, multicouche et multicomposant
US20180209069A1 (en) * 2015-07-27 2018-07-26 Drexel University Heterogeneous filaments,methods of producing the same, scaffolds, methods of producing the same, droplets, and methods of producing the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025083309A1 (fr) * 2023-10-17 2025-04-24 Administración General De La Comunidad Autónoma De Euskadi Dispositif d'extrusion intracorporel pour l'application de bio-encres, d'hydrogels ou de fluides en chirurgie laparoscopique

Also Published As

Publication number Publication date
WO2017143355A3 (fr) 2017-11-09

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