[go: up one dir, main page]

WO2017037535A3 - Systèmes et procédés pour détection de couleurs dans des systèmes de séquençage d'acide nucléique à haut débit - Google Patents

Systèmes et procédés pour détection de couleurs dans des systèmes de séquençage d'acide nucléique à haut débit Download PDF

Info

Publication number
WO2017037535A3
WO2017037535A3 PCT/IB2016/001318 IB2016001318W WO2017037535A3 WO 2017037535 A3 WO2017037535 A3 WO 2017037535A3 IB 2016001318 W IB2016001318 W IB 2016001318W WO 2017037535 A3 WO2017037535 A3 WO 2017037535A3
Authority
WO
WIPO (PCT)
Prior art keywords
systems
methods
nucleic acid
acid sequencing
color detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2016/001318
Other languages
English (en)
Other versions
WO2017037535A2 (fr
Inventor
Harald Quintel
Konstantin Lutze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen Instruments AG
Original Assignee
Qiagen Instruments AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Instruments AG filed Critical Qiagen Instruments AG
Priority to EP16785261.5A priority Critical patent/EP3344964A2/fr
Priority to JP2018529750A priority patent/JP2018533969A/ja
Priority to AU2016314054A priority patent/AU2016314054A1/en
Priority to CA2996476A priority patent/CA2996476A1/fr
Priority to CN201680050771.9A priority patent/CN108449958A/zh
Publication of WO2017037535A2 publication Critical patent/WO2017037535A2/fr
Publication of WO2017037535A3 publication Critical patent/WO2017037535A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/46—Measurement of colour; Colour measuring devices, e.g. colorimeters
    • G01J3/50—Measurement of colour; Colour measuring devices, e.g. colorimeters using electric radiation detectors
    • G01J3/51—Measurement of colour; Colour measuring devices, e.g. colorimeters using electric radiation detectors using colour filters
    • G01J3/513—Measurement of colour; Colour measuring devices, e.g. colorimeters using electric radiation detectors using colour filters having fixed filter-detector pairs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/30—Measuring the intensity of spectral lines directly on the spectrum itself
    • G01J3/36—Investigating two or more bands of a spectrum by separate detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/0875—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more refracting elements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/0875—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more refracting elements
    • G02B26/0883—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more refracting elements the refracting element being a prism
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G02B27/141—Beam splitting or combining systems operating by reflection only using dichroic mirrors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B5/00—Optical elements other than lenses
    • G02B5/20—Filters
    • G02B5/201—Filters in the form of arrays
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/10—Cameras or camera modules comprising electronic image sensors; Control thereof for generating image signals from different wavelengths
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N25/00—Circuitry of solid-state image sensors [SSIS]; Control thereof
    • H04N25/10—Circuitry of solid-state image sensors [SSIS]; Control thereof for transforming different wavelengths into image signals
    • H04N25/11—Arrangement of colour filter arrays [CFA]; Filter mosaics
    • H04N25/13—Arrangement of colour filter arrays [CFA]; Filter mosaics characterised by the spectral characteristics of the filter elements
    • H04N25/134—Arrangement of colour filter arrays [CFA]; Filter mosaics characterised by the spectral characteristics of the filter elements based on three different wavelength filter elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G01J2003/2826—Multispectral imaging, e.g. filter imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G01N2021/177—Detector of the video camera type
    • G01N2021/1776—Colour camera
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Optics & Photonics (AREA)
  • Signal Processing (AREA)
  • Multimedia (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Optical Filters (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La présente invention concerne un système optique d'instrument de séquençage possédant une source lumineuse combinée ayant de multiples faisceaux d'excitation colinéaires qui possèdent différentes longueurs d'onde d'excitation respectives, une surface de séquençage, qui comporte des matrices d'ADN et des marqueurs de nucléobase, conçue pour émettre une lumière d'émission respective à une longueur d'onde d'émission respective différente lors de l'excitation par un ou plusieurs des faisceaux d'excitation, un appareil de prise de vues en couleur conçu pour détecter la lumière d'émission de chacun des marqueurs de nucléobase, une première voie de passage optique conçue pour diriger les faisceaux d'excitation colinéaires de la source lumineuse combinée vers la surface de séquençage, et une seconde voie de passage optique conçue pour diriger la lumière d'émission de la surface de séquençage vers l'appareil de prise de vues en couleur.
PCT/IB2016/001318 2015-09-01 2016-08-31 Systèmes et procédés pour détection de couleurs dans des systèmes de séquençage d'acide nucléique à haut débit Ceased WO2017037535A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP16785261.5A EP3344964A2 (fr) 2015-09-01 2016-08-31 Systèmes et procédés pour détection de couleurs dans des systèmes de séquençage d'acide nucléique à haut débit
JP2018529750A JP2018533969A (ja) 2015-09-01 2016-08-31 ハイスループットシーケンシングにおける色検出のためのシステム及び方法
AU2016314054A AU2016314054A1 (en) 2015-09-01 2016-08-31 Systems and methods for color detection in high-throughput nucleic acid sequencing systems
CA2996476A CA2996476A1 (fr) 2015-09-01 2016-08-31 Systemes et procedes pour detection de couleurs dans des systemes de sequencage d'acide nucleique a haut debit
CN201680050771.9A CN108449958A (zh) 2015-09-01 2016-08-31 高通量核酸测序系统中用于颜色探测的系统和方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562212820P 2015-09-01 2015-09-01
US62/212,820 2015-09-01

Publications (2)

Publication Number Publication Date
WO2017037535A2 WO2017037535A2 (fr) 2017-03-09
WO2017037535A3 true WO2017037535A3 (fr) 2017-04-20

Family

ID=57190203

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2016/001318 Ceased WO2017037535A2 (fr) 2015-09-01 2016-08-31 Systèmes et procédés pour détection de couleurs dans des systèmes de séquençage d'acide nucléique à haut débit

Country Status (7)

Country Link
US (1) US20170058343A1 (fr)
EP (1) EP3344964A2 (fr)
JP (1) JP2018533969A (fr)
CN (1) CN108449958A (fr)
AU (1) AU2016314054A1 (fr)
CA (1) CA2996476A1 (fr)
WO (1) WO2017037535A2 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10228283B2 (en) * 2016-08-12 2019-03-12 Spectral Insights Private Limited Spectral imaging system
NL2020636B1 (en) * 2017-12-28 2019-07-08 Illumina Inc Light energy fluorescence excitation
CN109060738A (zh) * 2018-07-06 2018-12-21 广州蓝勃生物科技有限公司 一种多波长荧光即时检测仪及其检测方法
CN111272715B (zh) * 2018-12-04 2023-03-14 长春长光华大智造测序设备有限公司 一种基因测序仪的荧光成像系统
JP7348730B2 (ja) * 2019-02-22 2023-09-21 シスメックス株式会社 試料測定装置および試料測定方法
JP2022535884A (ja) * 2019-06-07 2022-08-10 ビーエーエスエフ コーティングス ゲゼルシャフト ミット ベシュレンクテル ハフツング 自然光及び／又は人工光の下での物体認識のためのシステム及び方法
US20220245842A1 (en) * 2019-06-07 2022-08-04 Basf Coatings Gmbh System and method for object recognition using fluorescent and antireflective surface constructs
CN110995965A (zh) * 2019-11-14 2020-04-10 维沃移动通信有限公司 一种拍摄模组及电子设备
US20210187503A1 (en) * 2019-12-19 2021-06-24 Personal Genomics Taiwan, Inc. Apparatus and system for single-molecule nucleic acids detection
EP3990891B1 (fr) * 2019-12-31 2025-12-03 Illumina, Inc. Fonctionnalité de mise au point automatique dans une analyse d'échantillon optique
WO2021189066A1 (fr) 2020-03-16 2021-09-23 Siemens Healthcare Diagnostics Inc. Appareil et procédés de discrimination optique adaptés pour surveiller des réactions
US11320380B2 (en) * 2020-04-21 2022-05-03 Sartorius Bioanalytical Instruments, Inc. Optical module with three or more color fluorescent light sources and methods for use thereof
US11526086B2 (en) * 2021-03-08 2022-12-13 Kla Corporation Multi-field scanning overlay metrology
KR102441156B1 (ko) * 2021-03-15 2022-09-07 한국광기술원 다파장 광원을 이용한 다중 분석장치
US11624656B2 (en) 2021-04-12 2023-04-11 Viavi Solutions Inc. Optical filter for an optical sensor device
US20230051226A1 (en) * 2021-08-10 2023-02-16 Mgi Tech Co., Ltd. Flow cell imaging systems and methods, and flow cells and other substrates for use in the same
US20250060311A1 (en) * 2021-11-12 2025-02-20 Bgi Shenzhen Method for Improving Sequencing Resolution, and Sequencing Apparatus and System
CN114134025B (zh) * 2021-11-19 2023-08-22 中国科学院长春光学精密机械与物理研究所 基因测序系统及其测序方法
KR102827153B1 (ko) * 2023-02-27 2025-06-30 심필관 광축이 정렬된 rgb 라인스캔 동축조명장치

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6185030B1 (en) * 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
US6995841B2 (en) * 2001-08-28 2006-02-07 Rice University Pulsed-multiline excitation for color-blind fluorescence detection
CA2588122A1 (fr) * 2004-11-16 2006-05-26 Helicos Biosciences Corporation Train optique et procede de detection et d'analyse tirf de molecule unique
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
GB2458855B (en) 2007-02-05 2011-06-08 Intelligent Bio Systems Inc A system for imaging treated bio-molecules in a flow-cell using a non-lasing light source
US20090298131A1 (en) 2008-02-19 2009-12-03 Intelligent Biosystems, Inc. Non-Emulsion Methods And Masked Biomolecules
JP2011523538A (ja) * 2008-05-20 2011-08-11 ペリカン イメージング コーポレイション 異なる種類の撮像装置を有するモノリシックカメラアレイを用いた画像の撮像および処理
JP5277082B2 (ja) * 2009-06-15 2013-08-28 株式会社日立ハイテクノロジーズ 蛍光分析方法
JP5964235B2 (ja) * 2009-11-30 2016-08-03 アイメックＩｍｅｃ スペクトル・イメージングシステム
CN102834718A (zh) * 2010-01-12 2012-12-19 耐克思乐生物科学有限责任公司 计数细胞和生物分子的系统和方法
WO2011112465A1 (fr) * 2010-03-06 2011-09-15 Illumina, Inc. Systèmes, procédés et appareils permettant de détecter des signaux optiques provenant d'un échantillon
CN103245611A (zh) * 2012-02-07 2013-08-14 鸿林堂科技股份有限公司 多重激发光源系统
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
CN104293648B (zh) * 2014-09-29 2016-08-24 大族激光科技产业集团股份有限公司 基因测序光路系统

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DAMIANA LEROSE ET AL: "CMOS-integrated geometrically tunable optical filters", APPLIED OPTICS, OPTICAL SOCIETY OF AMERICA, WASHINGTON, DC; US, vol. 52, no. 8, 10 March 2013 (2013-03-10), pages 1655 - 1662, XP001580634, ISSN: 0003-6935, DOI: HTTP://DX.DOI.ORG/10.1364/AO.52.001655 *
J. CHEN ET AL: "High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 111, no. 2, 30 December 2013 (2013-12-30), US, pages 664 - 669, XP055332961, ISSN: 0027-8424, DOI: 10.1073/pnas.1315735111 *
J. CHEN ET AL: "High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 111, no. 2, 30 December 2013 (2013-12-30), US, pages 664 - 669, XP055333277, ISSN: 0027-8424, DOI: 10.1073/pnas.1315735111 *
J. EID ET AL: "Real-Time DNA Sequencing from Single Polymerase Molecules", SCIENCE, vol. 323, no. 5910, 2 January 2009 (2009-01-02), pages 133 - 138, XP055333285, ISSN: 0036-8075, DOI: 10.1126/science.1162986 *
JOHN EID ET AL.: "Real-Time DNA Sequencing fromSingle Polymerase Molecules", SCIENCE, vol. 23, no. 5910, 20 November 2008 (2008-11-20), pages 133 - 138, XP055096175, ISSN: 0036-8075, DOI: 10.1126/science.1162986 *
NAN GUO ET AL: "CMOS Time-Resolved, Contact, and Multispectral Fluorescence Imaging for DNA Molecular Diagnostics", SENSORS, vol. 14, no. 11, 31 October 2014 (2014-10-31), pages 20602 - 20619, XP055333528, DOI: 10.3390/s141120602 *
SOBHY M A ET AL: "Versatile single-molecule multi-color excitation and detection fluorescence setup for studying biomolecular dynamics", REVIEW OF SCIENTIFIC INSTRUMENTS, AIP, MELVILLE, NY, US, vol. 82, no. 11, 1 November 2011 (2011-11-01), pages 113702 - 113702, XP012152143, ISSN: 0034-6748, [retrieved on 20111107], DOI: 10.1063/1.3657153 *

Also Published As

Publication number Publication date
CN108449958A (zh) 2018-08-24
AU2016314054A1 (en) 2018-03-15
WO2017037535A2 (fr) 2017-03-09
EP3344964A2 (fr) 2018-07-11
US20170058343A1 (en) 2017-03-02
CA2996476A1 (fr) 2017-03-09
JP2018533969A (ja) 2018-11-22

Similar Documents

Publication Publication Date Title
WO2017037535A3 (fr) Systèmes et procédés pour détection de couleurs dans des systèmes de séquençage d'acide nucléique à haut débit
EP4424846A3 (fr) Système optique et puce d'analyse pour sonder, détecter et analyser des molécules
MX378010B (es) Sistema óptico y chip de ensayo para sondear, detectar y analizar moléculas.
MX2016016248A (es) Sistema para evitar bioensuciamiento.
MX384725B (es) Dispositivo integrado con fuente de luz externa para el sondeo, detección y análisis de moléculas.
EP4517318A3 (fr) Système optique pour électrophorèse capillaire
CY1122788T1 (el) Ανιχνευση πυρηνικου οξεος στοχου και παραλλαγματων
MX392170B (es) Método para determinar la secuencia de un acido nucleico usando luminiscencia resuelta por tiempo.
MX2016012508A (es) Detección de secuencia por objetivo de ácido nucleico usando diferentes temperaturas de detección.
EP4428528A3 (fr) Compositions nucléotidiques marquées et procédés de séquençage d'acides nucléiques
CY1125046T1 (el) Συστημα κατα της βιολογικης ρυπανσης, ελεγκτης και μεθοδος ελεγχου του συστηματος κατα της βιολογικης ρυπανσης
WO2014190322A3 (fr) Analyse d'acides nucléiques basés sur des nanopores avec une détection par fret mixte
JP2016524469A5 (fr)
WO2015126766A8 (fr) Procédés et compositions utilisables en vue du profilage adn
SG10201909512XA (en) Multiplexed, continuous-flow, droplet-based platform for high-throughput genetic detection
DE602006021554D1 (de) Multiplex-fluoreszenznachweisvorrichtung mit mehreren über ein faserbündel an einen gemeinsamen detektor gekoppelten optischen modulen
BR112018011649A2 (pt) dispositivo, kit e método para verificar um pneu
BR112021022865A2 (pt) Matriz e método para detectar informações espaciais de ácidos nucleicos
MX2021011433A (es) Sistema.
WO2014130970A3 (fr) Systèmes et procédés optiques pour l'analyse biologique
GB201119334D0 (en) Improved ambient light sensing system and method
WO2017072401A3 (fr) Élément capteur
BR112014030255A2 (pt) módulo de matriz de elemento emissor de luz, método de controle de chips de matriz de elementos emissores de luz, meio de gravação legível por computador não transitório, e aparelho formador de imagem
WO2017044801A3 (fr) Procédés pour identifier des cibles polypeptidiques modulées par des médicaments en vue d'une dégradation
BR112017013327A2 (pt) método e aparelho para verificar pneus em uma linha de produção de pneu.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 16785261

Country of ref document: EP

Kind code of ref document: A2

ENP Entry into the national phase

Ref document number: 2996476

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2018529750

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2016314054

Country of ref document: AU

Date of ref document: 20160831

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2016785261

Country of ref document: EP