[go: up one dir, main page]

WO2017028340A1 - Procédé de commande de cellule unique basé sur une technique de diélectrophorèse induite par la lumière - Google Patents

Procédé de commande de cellule unique basé sur une technique de diélectrophorèse induite par la lumière Download PDF

Info

Publication number
WO2017028340A1
WO2017028340A1 PCT/CN2015/088944 CN2015088944W WO2017028340A1 WO 2017028340 A1 WO2017028340 A1 WO 2017028340A1 CN 2015088944 W CN2015088944 W CN 2015088944W WO 2017028340 A1 WO2017028340 A1 WO 2017028340A1
Authority
WO
WIPO (PCT)
Prior art keywords
light
ito glass
control method
cell control
induced dielectrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2015/088944
Other languages
English (en)
Chinese (zh)
Inventor
李志�
张光烈
李文荣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen University
Original Assignee
Shenzhen University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen University filed Critical Shenzhen University
Publication of WO2017028340A1 publication Critical patent/WO2017028340A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Definitions

  • the invention relates to the field of single cell dynamics, in particular to a single cell control method based on light induced dielectrophoresis.
  • Single cell control is an interdisciplinary frontier that analyzes the development of penetration between chemistry, biology, and medicine.
  • the existing single cell control technology mainly includes patch clamp combined with atomic force microscopy technology. Langer of the University of Tübingen in Germany took the lead in experimenting with patch clamp technology and atomic force microscopy (AFM) in 1997. In 2001, Zhang of New York University used a patch clamp/atomic force microscope system to study cell-specific membrane motion, membrane potential, and ion current measurement functions. In 2008, Pamir et al. of the University of Kunststoff in Germany combined atomic force microscopy with planar patch clamp technique to study the relationship between external mechanical stimulation and membrane potential and ion channel current on lymphocytes.
  • atomic force microscopy can only provide an external stimulus, no more uses, which makes the way to provide external mechanical stimulation is relatively simple. And its operation is very cumbersome, costly and time consuming.
  • the object of the present invention is to provide a single cell control method based on light-induced dielectrophoresis technology, which aims to solve the problem that the existing single cell control method is very cumbersome, costly, time consuming and has no vision. Feedback questions.
  • a single cell control method based on light-induced dielectrophoresis technology comprising the steps of:
  • a photo-induced dielectrophoresis chip is prepared.
  • the photo-induced dielectrophoresis chip has a three-layer structure: a three-layer structure: the lower layer is ITO glass coated with a hydrogenated amorphous silicon coating, and the upper layer is ITO glass without coating.
  • a microfluidic channel is encapsulated between the upper and lower layers of ITO glass for injecting a solution for the desired operation;
  • the single cell control method based on the light-induced dielectrophoresis technique wherein the step of fabricating the light-induced dielectrophoresis chip in the step A specifically includes:
  • A2 depositing a hydrogenated amorphous silicon coating on the ITO glass substrate
  • a conductive adhesive is applied to the area of the ITO glass substrate that is not covered with the hydrogenated amorphous silicon coating.
  • F DEP is the average dielectrophoretic force acting on the cell
  • R is the radius of the cell
  • ⁇ m is the dielectric constant of the solution in which the cell is located
  • E rms is the root mean square value of the applied AC signal
  • f CM is Clausius-Mossotti Factor, the real part of the factor Re[f CM ] is taken when calculating the average dielectrophoretic force.
  • the single cell control method based on light-induced dielectrophoresis technology wherein the f CM factor is defined as follows:
  • ⁇ p * and ⁇ m * are the complex dielectric constants of the cells and solutions, respectively.
  • ⁇ is the dielectric constant of the solution
  • ⁇ is the conductivity
  • ⁇ is the frequency of the applied AC signal
  • E is the electric field strength
  • ⁇ is the viscosity of the solution
  • IM[f CM ] is the imaginary part of the Clausius-Mossotti factor
  • K is the coefficient
  • the present invention has the following advantages: First, the cost is low, and the light-induced dielectrophoresis platform used in the present invention is low in cost. Second, the operation is simple, the entire control process is basically automated, and only the cultured cells are placed in the container, and other processes are all completed by software. Third, the efficiency is high, and the present invention can perform a large number of cell operations in a short period of time due to the automation of the control process. Fourth, high-precision real-time operation, real-time manipulation of cells through visual feedback, improving the accuracy of operation.
  • FIG. 1 is a flow chart of a preferred embodiment of a single cell control method based on photoinduced dielectrophoresis.
  • FIG. 2 is a schematic view showing the structure of a light-induced dielectrophoresis platform in the present invention.
  • the present invention provides a single cell control method based on a photoinduced dielectrophoresis technique, and the present invention will be further described in detail below in order to make the objects, technical solutions and effects of the present invention more clear and clear. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
  • FIG. 1 is a flow chart of a preferred embodiment of a single cell control method based on a light-induced dielectrophoresis technique. As shown in the figure, the method includes the following steps:
  • a light-induced dielectrophoresis chip (ODEP chip), wherein the light-induced dielectrophoresis chip has a three-layer structure: the lower layer is ITO glass coated with a hydrogenated amorphous silicon coating, and the upper layer is uncoated (ie, does not contain hydrogenation) ITO glass coated with amorphous silicon, encapsulating a microfluidic channel between the upper and lower ITO glass for injecting the solution required for operation;
  • the step of fabricating the light-induced dielectrophoresis chip specifically includes:
  • a layer of hydrogenated amorphous silicon was deposited on the surface of the ITO glass substrate to a thickness of 1 micron.
  • the stencil is to make a cover according to the specified pattern, the cover is placed on the surface of the photoresist, and the cover is irradiated with ultraviolet rays, and the uncovered photoresist is dissolved under the action of ultraviolet rays, and finally the photoresist having the same shape as the cover is obtained.
  • Floor is to make a cover according to the specified pattern, the cover is placed on the surface of the photoresist, and the cover is irradiated with ultraviolet rays, and the uncovered photoresist is dissolved under the action of ultraviolet rays, and finally the photoresist having the same shape as the cover is obtained.
  • a microfluidic channel is encapsulated between the upper and lower layers of ITO glass, specifically a microfluidic channel is encapsulated by PDMS or double-sided tape.
  • a light-induced dielectrophoresis platform is first constructed.
  • the platform also requires an optical microscope 10, an optical projector (high resolution), a programmable signal generation circuit, and a host system to form a microscope image system.
  • the host system includes: an image acquisition module, a microscopic vision algorithm processing module, a biochip drive controller, a virtual electrode generation module, and a display output module.
  • the image acquisition module is configured to acquire an image of the optical microscope 10, and is processed by a microscopic vision algorithm processing module and displayed by a display output module, and the microscopic vision algorithm processing module also drives the biochip driver controller and The virtual electrode generation module signals to control the operation of both.
  • the biochip drive controller is coupled to the programmable signal generation circuit to vary the signal frequency and magnitude.
  • the programmable signal generating circuit connects the ODEP chip 20 through electrodes.
  • the optical projector is disposed below the ODEP chip 20 for illuminating the incident light.
  • the virtual electrode generating module is connected to the projector.
  • optical microscope parameters are as follows:
  • Electric focus can move up and down (upper 13mm / 2mm);
  • Concentrator waterproof, working distance: 7.2mm;
  • Objective lens 20x, highly achromatic lens, nanocrystalline coating
  • Fluorescence filter set FITC/GFP.
  • the biochip driver controller can send a signal to the programmable signal generation circuit, and then the programmable signal generation circuit inputs the variable frequency AC signal to the electrodes of the upper and lower layers of the ITO glass, and the optical projector utilizes the incident.
  • the programmable signal generation circuit inputs the variable frequency AC signal to the electrodes of the upper and lower layers of the ITO glass, and the optical projector utilizes the incident.
  • step S300 under the real-time observation of the microscope image system, by changing the frequency and size of the alternating current signal, the direction and size of the dielectrophoretic force received by the cell are changed to control the direction of cell movement, and high-speed real-time manipulation is realized.
  • Nano entity under the real-time observation of the microscope image system, by changing the frequency and size of the alternating current signal, the direction and size of the dielectrophoretic force received by the cell are changed to control the direction of cell movement, and high-speed real-time manipulation is realized.
  • the following focuses on how to control the direction of cell movement by changing the frequency and size of the AC signal.
  • F DEP is the average dielectrophoretic force acting on the cell
  • R is the radius of the cell
  • ⁇ m is the dielectric constant of the solution in which the cell is located
  • E rms is the root mean square value of the applied electric field (AC signal)
  • f CM is Clausius-Mossotti factor
  • Re[f CM ] is taken when calculating the average dielectrophoretic force, which is defined as follows:
  • Equation 2 ⁇ p * and ⁇ m * are the complex permittivity of the cell and the solution, respectively, and the complex permittivity (including ⁇ p * and ⁇ m *) in Equation 2 can be expressed as:
  • ⁇ is the dielectric constant of the solution
  • ⁇ is the conductivity
  • ⁇ is the frequency of the applied electric field (alternating current signal).
  • f CM is a frequency dependent variable factor. Considering the alternating electric field with different frequencies, when the dielectrophoretic force and the electric field intensity change direction are the same, it is called positive dielectrophoresis; when the dielectrophoretic force and the electric field intensity change direction are opposite, it is called negative dielectrophoresis. Therefore, by changing the frequency of the applied electric field, the direction of the dielectrophoretic force to which the cells are subjected can be changed to achieve the purpose of controlling the direction of cell movement.
  • E is the electric field strength (AC signal strength)
  • IM[f CM ] is the imaginary part of the Clausius-Mossotti factor
  • K is the coefficient
  • ⁇ is the viscosity of the solution.
  • the dielectric properties of the cells can be estimated based on the relationship between the rotational speed of the cells and the dielectric constant of the cells.
  • the strength and direction of the dielectrophoretic force that a cell receives depends primarily on the dielectric properties of the medium and the cell, such as shape, size, and electric field frequency.
  • the present invention utilizes light-induced dielectrophoretic force (ODEP) (when a certain frequency band is applied, a dominant force in electro-hydraulics) to identify and manipulate biological cells, and to separate nanoscale polymer particles.
  • ODEP light-induced dielectrophoretic force
  • the ODEP chip is driven by a variable frequency AC signal, and the AC signal is input through the conductive contacts of the upper and lower layers of ITO glass. At this time, only a small portion of the solution layer is divided and a uniform electric field is generated in the solution layer.
  • the optical conductivity of a-Si:H increases by several orders of magnitude due to the increase in the number of electron-hole pairs. Due to the reduced resistance of the incident light region, in the solution layer The partial pressure will be greatly increased, so that the a:Si:H in the incident light region will become an effective virtual electrode to generate a non-uniform electric field.
  • This light-induced, non-uniform electric field produces a dielectrophoretic force, ie, light-induced dielectrophoretic force (ODEP), of the particles in the polarized region.
  • Programmatic dynamic motion is achieved through optical microscopy and host systems, and automated capture, manipulation, separation and assembly of micro-nano entities are achieved without any manual interface. Therefore, the ODEP chip of the present invention can provide a method for efficiently realizing high-speed manipulation of micro-nano entities.
  • the present invention has the following advantages: First, the cost is low, and the light-induced dielectrophoresis platform used in the present invention is low in cost. Second, the operation is simple, the entire control process is basically automated, and only the cultured cells are placed in the container, and other processes are all completed by software. Third, the efficiency is high, and the present invention can complete the classification of a large number of cells in a short period of time due to the automation of the control process. Fourth, high-precision real-time operation, real-time manipulation of cells through visual feedback, improving the accuracy of operation.
  • the method of the invention solves the problem that the traditional dielectrophoresis chip requires complex and fine electrode processing, and dynamically generates different shapes of virtual electrodes through an optical projection device, thereby generating a non-uniform electric field, and the dielectrophoretic force acts on the micro-nano Particles, real-time manipulation of micro-nano particles, and real-time image output.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé de commande de cellule unique basé sur une technique de diélectrophorèse induite par la lumière, comprenant les étapes consistant à : A. fabriquer une puce de diélectrophorèse induite par la lumière (20), la puce de diélectrophorèse induite par la lumière (20) étant composée d'une structure à trois couches : la couche inférieure étant du verre ITO revêtu d'une couche de revêtement de silicium amorphe hydrogéné, la couche supérieure étant du verre ITO sans couche de revêtement, et un canal micro-fluidique étant encapsulé entre les couches de verre ITO supérieure et inférieure pour injecter une solution nécessaire pour des opérations ; B. entrer un signal en courant alternatif à fréquence variable sur des électrodes des couches de verre ITO supérieure et inférieure, et en même temps utiliser une lumière incidente pour irradier la puce de diélectrophorèse induite par la lumière (20), de manière à générer un champ électrique non uniforme dans une zone irradiée ; et C. sous l'observation en temps réel d'un système d'image par microscope, réaliser une commande de cellule en changeant la fréquence et l'amplitude du signal en courant alternatif. Le procédé de commande présente les avantages d'un faible coût, d'une utilisation simple et d'une efficacité élevée.
PCT/CN2015/088944 2015-08-14 2015-09-06 Procédé de commande de cellule unique basé sur une technique de diélectrophorèse induite par la lumière Ceased WO2017028340A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510501121.9 2015-08-14
CN201510501121.9A CN105092679B (zh) 2015-08-14 2015-08-14 一种基于光诱导介电泳技术的单细胞控制方法

Publications (1)

Publication Number Publication Date
WO2017028340A1 true WO2017028340A1 (fr) 2017-02-23

Family

ID=54573587

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2015/088944 Ceased WO2017028340A1 (fr) 2015-08-14 2015-09-06 Procédé de commande de cellule unique basé sur une technique de diélectrophorèse induite par la lumière

Country Status (2)

Country Link
CN (1) CN105092679B (fr)
WO (1) WO2017028340A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111500440A (zh) * 2020-04-26 2020-08-07 中国科学院广州生物医药与健康研究院 一种单细胞分选装置和单细胞分选方法
CN111908421A (zh) * 2020-07-31 2020-11-10 江南大学 基于光诱导介电泳的微纳自组装操作方法及系统
CN112808121A (zh) * 2019-11-17 2021-05-18 海南大学 一种光诱导电渗流混合方法
CN113189180A (zh) * 2021-03-29 2021-07-30 大连海事大学 一种基于交流-介电泳的微藻表征与识别装置及方法
CN114774275A (zh) * 2022-03-21 2022-07-22 西北工业大学深圳研究院 基于旋转电场下双极性电极的三维细胞球生成芯片及应用
CN115703078A (zh) * 2021-08-12 2023-02-17 长春理工大学 基于光诱导介电泳技术的虚拟微通道操纵微纳物体的方法

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106190830B (zh) * 2016-08-02 2019-06-04 河海大学常州校区 用于控制细胞作三维旋转的实验系统及其工作方法
CN106978334B (zh) * 2017-04-13 2019-07-30 东南大学 一种基于光诱导介电泳技术和纳米孔的dna测序装置和测序方法
CN107858289B (zh) * 2017-12-25 2019-06-21 上海大学 一种细胞划痕芯片、装置及方法
CN109337796A (zh) * 2018-11-15 2019-02-15 江南大学 酵母菌细胞高效筛选系统及其ito玻璃操作模块
CN109682745B (zh) * 2019-01-07 2020-07-07 清华大学 一种单细胞参数测量方法及装置
CN109679845B (zh) * 2019-01-16 2020-09-22 江南大学 基于光诱导介电泳技术构建的微生物细胞工厂及其应用
CN112870974A (zh) * 2019-11-29 2021-06-01 海南大学 一种基于连续流的光诱导介电泳微粒分离方法
CN112316994B (zh) * 2020-11-06 2022-03-01 江南大学 酵母菌的一体化检测芯片及方法
CN114689840B (zh) * 2020-12-31 2025-05-09 彩科(苏州)生物科技有限公司 一种荧光编码微球的分选方法
CN112814862A (zh) * 2021-01-12 2021-05-18 平顶山学院 一种基于藻类细胞驱动的发电装置及其工作方法
CN113312829B (zh) * 2021-05-26 2022-07-26 江南大学 基于数据驱动odep运动学模型的微纳米粒子移动控制方法
CN113249216A (zh) * 2021-06-04 2021-08-13 宁夏医科大学总医院 一种基于光诱导介电泳技术的细菌分离装置
CN113308372A (zh) * 2021-06-10 2021-08-27 深圳大学 微粒操纵与检测芯片及其制作方法、微粒操纵与检测方法
CN113740234B (zh) * 2021-09-17 2024-11-26 苏州先米科技有限公司 一种微筛过滤器
CN114107025B (zh) * 2021-11-30 2023-11-24 平顶山学院 基于藻类细胞的流体驱动方法
CN114686373A (zh) * 2022-04-12 2022-07-01 安图实验仪器(郑州)有限公司 一种微生物固定方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201247242Y (zh) * 2008-08-15 2009-05-27 东南大学 微纳生物粒子介电表征装置
WO2015058265A1 (fr) * 2013-10-25 2015-04-30 Monash University Déplacement latéral déterministe virtuel pour une séparation de particules à l'aide d'ondes acoustiques de surface
CN105044192A (zh) * 2015-08-14 2015-11-11 深圳大学 一种基于光诱导介电泳技术的细胞分类方法
CN105184853A (zh) * 2015-08-14 2015-12-23 深圳大学 一种基于光流分析的单细胞三维图像生成方法
CN105243673A (zh) * 2015-08-05 2016-01-13 深圳大学 一种基于块匹配的运动估计方法、运动估计系统及其应用
CN105241940A (zh) * 2015-08-05 2016-01-13 深圳大学 一种基于介电泳力场的药效检测方法及其系统

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201075104Y (zh) * 2007-07-13 2008-06-18 东南大学 基于复合介电泳的单细胞介电谱自动测试装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201247242Y (zh) * 2008-08-15 2009-05-27 东南大学 微纳生物粒子介电表征装置
WO2015058265A1 (fr) * 2013-10-25 2015-04-30 Monash University Déplacement latéral déterministe virtuel pour une séparation de particules à l'aide d'ondes acoustiques de surface
CN105243673A (zh) * 2015-08-05 2016-01-13 深圳大学 一种基于块匹配的运动估计方法、运动估计系统及其应用
CN105241940A (zh) * 2015-08-05 2016-01-13 深圳大学 一种基于介电泳力场的药效检测方法及其系统
CN105044192A (zh) * 2015-08-14 2015-11-11 深圳大学 一种基于光诱导介电泳技术的细胞分类方法
CN105184853A (zh) * 2015-08-14 2015-12-23 深圳大学 一种基于光流分析的单细胞三维图像生成方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
NI, ZHONGHUA ET AL.: "gu? ngyòud? ojièdiàny? ng w?ic?ozòng guòchéngz?ng degu? ngdi? nxuánzhu? n", SCIENTIA SINICA(TECHNOLOGICA, vol. 42, 31 January 2012 (2012-01-31), pages 91 - 97, ISSN: 1674-7259 *
SONG, CHUNFENG ET AL.: "Single Micro-particle Manipulation Based on Light-induced Dielectrophoresis", JOURNAL OF MECHANICAL ENGINEERING, vol. 46, no. 7, 30 April 2010 (2010-04-30), pages 149 - 152, ISSN: 0577-6686 *
WANG, SHU'E ET AL.: "Automatic Manipulation of Polystyrene Beads via Optically-Induced Dielectrophoresis", MICRONANOELECTRONIC TECHNOLOGY, vol. 48, 28 February 2011 (2011-02-28), pages 2, ISSN: 1671-4776 *
ZHU, XIAOLU ET AL.: "j?yúgu? ngyòud? ojièdiàny? ng de w? ilìz? guòl?, sh?yùn, fùjí, héjùji?o deshíyànyánji?", SCIENTIA SINICA (TECHNOLOGICA, vol. 41, 31 March 2011 (2011-03-31), pages 334 - 337, ISSN: 1674-7259 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112808121A (zh) * 2019-11-17 2021-05-18 海南大学 一种光诱导电渗流混合方法
CN111500440A (zh) * 2020-04-26 2020-08-07 中国科学院广州生物医药与健康研究院 一种单细胞分选装置和单细胞分选方法
CN111908421A (zh) * 2020-07-31 2020-11-10 江南大学 基于光诱导介电泳的微纳自组装操作方法及系统
CN111908421B (zh) * 2020-07-31 2024-01-05 江南大学 基于光诱导介电泳的微纳自组装操作方法及系统
CN113189180A (zh) * 2021-03-29 2021-07-30 大连海事大学 一种基于交流-介电泳的微藻表征与识别装置及方法
CN115703078A (zh) * 2021-08-12 2023-02-17 长春理工大学 基于光诱导介电泳技术的虚拟微通道操纵微纳物体的方法
CN114774275A (zh) * 2022-03-21 2022-07-22 西北工业大学深圳研究院 基于旋转电场下双极性电极的三维细胞球生成芯片及应用

Also Published As

Publication number Publication date
CN105092679A (zh) 2015-11-25
CN105092679B (zh) 2018-07-03

Similar Documents

Publication Publication Date Title
WO2017028340A1 (fr) Procédé de commande de cellule unique basé sur une technique de diélectrophorèse induite par la lumière
CN105044192B (zh) 一种基于光诱导介电泳技术的细胞分类方法
Jeon et al. Self-cleaning hybrid energy harvester to generate power from raindrop and sunlight
CN105184853B (zh) 一种基于光流分析的单细胞三维图像生成方法
CN107574163B (zh) 一种基于光诱导介电泳装置筛选被磁性纳米粒子修饰细胞的方法
CN201247242Y (zh) 微纳生物粒子介电表征装置
CN103294275A (zh) 一种非接触式柔性控制器及其制备方法
CN102174369A (zh) 一种基于微狭缝结构的全pdms微流控细胞捕获芯片及其制法
CN101275944B (zh) 单细胞行波介电谱的高通量测试芯片及测试方法
CN103030097A (zh) 基于静电场自聚焦的圆片级低维纳米结构的制备方法
CN104371917A (zh) 一种基于双光电薄膜生物芯片诱导细胞成链的新方法
CN201188104Y (zh) 高通量测试芯片
Gan et al. Non-contact massively parallel manipulation of micro-objects by optoelectronic tweezers
Karg et al. Electrochemical grippers based on the tuning of surface forces for applications in micro-and nanorobotics
Lee et al. Rapid fabrication of nanoparticles array on polycarbonate membrane based on positive dielectrophoresis
CN104096609A (zh) 一种胶体晶体纸芯片及制备方法
CN215050149U (zh) 一种基于微流控芯片的细胞分选与识别系统
CN113249216A (zh) 一种基于光诱导介电泳技术的细菌分离装置
Wu et al. High efficiency light-induced dielectrophoresis biochip prepared using CVD techniques
Qu et al. Manipulation of living cells by optically induced dielectrophoresis
Cai et al. Recent advances in optically induced di-electrophoresis and its biomedical applications
CN112870974A (zh) 一种基于连续流的光诱导介电泳微粒分离方法
CN113308340A (zh) 一种基于微流控芯片的细胞分选与识别系统
CN104891424B (zh) 一种驱使碳纳米管成链的新型平行电场式光电芯片
CN215586515U (zh) 一种基于光电子镊的五通道微粒分离装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15901544

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 12/07/2018)

122 Ep: pct application non-entry in european phase

Ref document number: 15901544

Country of ref document: EP

Kind code of ref document: A1