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WO2017019300A1 - Filaments hétérogènes, procédés de production de ceux-ci, échafaudages, procédés de production de ceux-ci, gouttelettes, et procédés de production de celles-ci - Google Patents

Filaments hétérogènes, procédés de production de ceux-ci, échafaudages, procédés de production de ceux-ci, gouttelettes, et procédés de production de celles-ci Download PDF

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Publication number
WO2017019300A1
WO2017019300A1 PCT/US2016/042032 US2016042032W WO2017019300A1 WO 2017019300 A1 WO2017019300 A1 WO 2017019300A1 US 2016042032 W US2016042032 W US 2016042032W WO 2017019300 A1 WO2017019300 A1 WO 2017019300A1
Authority
WO
WIPO (PCT)
Prior art keywords
filament
heterogeneous
solution
cross
droplet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2016/042032
Other languages
English (en)
Inventor
Wei Sun
Qudus Hamid
Jessica Snyder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Drexel University
Original Assignee
Drexel University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Drexel University filed Critical Drexel University
Priority to CA2993779A priority Critical patent/CA2993779A1/fr
Priority to US15/745,299 priority patent/US20180209069A1/en
Priority to CN201680043828.2A priority patent/CN107847644A/zh
Priority to EP16831021.7A priority patent/EP3328456A4/fr
Publication of WO2017019300A1 publication Critical patent/WO2017019300A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/28—Formation of filaments, threads, or the like while mixing different spinning solutions or melts during the spinning operation; Spinnerette packs therefor
    • D01D5/30—Conjugate filaments; Spinnerette packs therefor
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H3/00—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length
    • D04H3/08—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length characterised by the method of strengthening or consolidating
    • D04H3/14—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length characterised by the method of strengthening or consolidating with bonds between thermoplastic yarns or filaments produced by welding
    • D04H3/147—Composite yarns or filaments
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/38—Formation of filaments, threads, or the like during polymerisation

Definitions

  • One aspect of the invention provides a method of producing a heterogeneous filament.
  • the plurality of filament components can be selected from the group consisting of: 2, 3, 4, 5, 6, 7, 8, 9, and 10.
  • the heterogeneous filament can have a one-dimensional pattern.
  • the heterogeneous filament can have a two-dimensional pattern.
  • the heterogeneous filament can have a three- dimensional pattern.
  • the heterogeneous filament can be symmetrical along a longitudinal axis.
  • the heterogeneous filament can be asymmetrical along a longitudinal axis.
  • the heterogeneous filament can have a largest cross-sectional dimension less than about 1 mm.
  • the heterogeneous filament can have a largest cross-sectional dimension less than about 1 ⁇ .
  • Another aspect of the invention provides a method of fabricating a scaffold.
  • the method includes producing a plurality of filaments according to the methods described herein.
  • Another aspect of the invention provides a heterogeneous filament including a plurality of adj acent filament components.
  • the plurality of filament components can contact each other in a common outlet channel, but remain substantially unmixed.
  • the plurality of filament components can contact each other in a common outlet channel and mix.
  • the plurality of filament components can contact each other in a common outlet channel and mix to form one or more concentration gradients between adjacent filament components.
  • the plurality of filament components can be selected from the group consisting of: 2, 3, 4, 5, 6, 7, 8, 9, and 10.
  • the plurality of filament components can include one or more selected from the group consisting of: a polymer, a solution, a cell-laden solution, a chemically reactive solution, an aqueous solution, sodium alginate solutions, a sacrificial support material, a cell, alginate, a cross-linker, a cross-linking solution, a calcium chloride solution, and a hydrogel.
  • the cross-linking liquid can be a calcium chloride solution.
  • the calcium chloride solution can have a concentration between about 0.3 g/L and about 0.5 g/L.
  • the calcium chloride solution can have a concentration between about 0.5 g/L and about 1.0 g/L.
  • the calcium chloride solution can have a concentration greater than 1.0 g/L.
  • the one or more liquids can include one or more selected from the group consisting of: a polymer, a solution, a cell-laden solution, a chemically reactive solution, an aqueous solution, sodium alginate solutions, a sacrificial support material, a cell, alginate, a cross-linker, a cross- linking solution, a calcium chloride solution, and a hydrogel.
  • the positioning step can include positioning the printing tip within about 100 ⁇ of a surface of the cross-linking liquid.
  • the droplet can be a heterogeneous droplet and the applying pressure step can include applying pressure to a plurality of liquids.
  • the droplet can have a substantially spherical shape.
  • the droplet can have a substantially toroidal shape.
  • the droplet can have a substantially flat and circular shape.
  • FIG. 2 depicts unit cell designs of fiber cross-sections printed according to embodiments of the invention.
  • FIG. 5 is a photograph of a fabricated SMMB deposition head perfused with three different materials for visualization purposes according to an embodiment of the invention.
  • FIG. 6 depicts a method for fabricating an SMMB deposition head using precision extrusion deposition (PED) and replica molding processes according to an embodiment of the invention.
  • PED precision extrusion deposition
  • FIG. 8 presents photographs of a three material deposition head according to an embodiment of the invention.
  • FIG. 9 presents photographs of the SMMB deposition channel during volume fraction adjustments according to an embodiment of the invention.
  • FIG. 10 provides photographs of extrusion along a tool path to build a free-standing scaffold (left) and extrusion over a controlled build cycle to print droplets (right) according to an embodiment of the invention.
  • FIG. 11 provides a schematic of a synchronized multi -material deposition head mounted on a motion system printing heterogonous line with process control of the volume fraction of each material according to an embodiment of the invention.
  • FIG. 13 is a schematic of droplet-forming process stages according to an embodiment of the invention.
  • FIGS. 15A and 15B provide photographs of red and green alginate accumulating on tip of deposition head as nano-liter droplet according to an embodiment of the invention. Photographs are color thresholded to present separate streams of red and green.
  • FIG. 17 provides an analytical model for printing heterogeneous nano-liter droplets according to an embodiment of the invention.
  • Microfluidic techniques combine multiple material inlets in a single outlet channel without mixing due to low inertial forces in micro-scale cross-sectional channels.
  • Embodiments of the deposition head can leverage non-mixing microfluidic flow to combine multiple materials in a heterogeneous array prior to deposition.
  • Embodiments of the invention improve printing resolution over multi-nozzle deposition (MND) and enable mass transfer and chemical reactions, such as partial cross-linking, prior to deposition.
  • MND multi-nozzle deposition
  • Embodiments of the invention can produce structures such as scaffolds through the following process.
  • component materials can be selected based on composition of the target material, functional tissue, support material, and requirement for cross-linking solution.
  • the arrangement of the materials can be defined based on cell-to-cell contact in vivo and construction requirements.
  • the volume fraction of each constituent component can be dynamically varied during the build cycle and is not restricted by the channel network in the deposition head.
  • the layout of the channel network can be defined to assemble the materials in a heterogeneous or axisymmetric style as depicted in FIG. 1.
  • FIG. 2 depicts unit cell designs of fiber cross-sections printed using embodiments of the invention.
  • Filaments can be composed of a variety of liquids including solutions (e.g., cell- laden, chemically reactive, and the like) and sacrificial support material to produce void spaces.
  • solutions e.g., cell- laden, chemically reactive, and the like
  • sacrificial support material to produce void spaces.
  • the volume fraction of each solution can be tuned using the flow rate controlled by
  • programmable syringe pumps Assembly of solutions can be controlled by a microfluidic system.
  • Two-dimensional microfluidic systems can produce 1 -dimensional array of solutions.
  • a more sophisticated three-dimensional microfluidic system can produce a 2-dimensional array of solutions.
  • Exemplary channel networks to combine multiple materials in a 1 -dimensional array are depicted in FIG. 3.
  • FIG. 4 provides an exemplary block diagram of a synchronized multi-material bioprinter (SMMB) integrating a deposition head for controlled heterogeneous extrusion with a multi- nozzle deposition (MND) system.
  • SMMB synchronized multi-material bioprinter
  • MND multi- nozzle deposition
  • FIG. 5 is a photograph of a fabricated SMMB deposition head perfused with three different materials for visualization purposes.
  • FIG. 6 depicts a method for fabricating an SMMB deposition head using precision extrusion deposition (PED) and replica molding processes according to an embodiment of the invention.
  • PED precision extrusion deposition
  • FIG. 7 depicts various design factors associated with printing heterogeneous cell-laden constructs using the SMMB deposition head.
  • heterogamous filaments enable layer-by-layer fabrication of a free-standing scaffold or controlled cell seeding in channels of a microfluidic device deposited along a tool path.
  • Multiple deposition heads can be simultaneously integrated in a single SMMB system. Multiple integrated deposition heads offer the same benefit as multi-nozzle printing including supplemental cross-linking solutions or secondary heterogeneous set of materials.
  • the synchronized multi -material bioprinter (SMMB) dispensing system can be integrated with the existing multi-nozzle deposition (MND) system to continuously extrude heterogeneous filaments along a tool path.
  • SMMB system performance to pattern a heterogeneous filament along a tool path can be defined by a set of geometric design variables to control printed filament cross-sectional width/height, reaction time in the outlet channel.
  • Design variables ⁇ ⁇ , ⁇ can be defined by droplet volume, V D , and volume fraction of material i to material j, ⁇ .
  • FIG. 11 provides a schematic of a synchronized multi -material deposition head mounted on a motion system printing heterogenous line with process control of the volume fraction of each material.
  • FIG. 11 also depicts the internal architecture of an exemplary printed filament.
  • FIG. 12 depicts a heterogeneous printed filament in a square wave pattern printed using a bioprinter and a multi-material deposition head with two inlet channels of red and green solution.
  • a schematic of the microfluidic network in multi-material deposition head depicts the red and green flows combined in a single outlet channel.
  • the bioprinter' s motion system carries the deposition head over the stationary substrate to produce the square wave pattern.
  • a photograph of the printed alginate filament in square wave pattern (A) and binary contrast enhancement of green (B) and red (C) alginate are shown.
  • Solutions can be prepared and loaded into the SMMB material delivery system syringe pump for controlled extrusion during the build cycle.
  • sodium alginate aqueous solutions of alginic acid sodium salt from brown algae and a cross-linking solution of ACS grade calcium chloride were prepared from distilled water, respectively.
  • Alginate solution was loaded into the SMMB material delivery system and a cross-linking solution flooded the reservoir pool positioned below the printing tip.
  • the SMMB deposition head outlet tip was lowered to less than 100 ⁇ above cross-linking pool.
  • the programmable syringe pump extruded alginate through the deposition head. The alginate accumulates on the tip, until contact with the cross- linking reservoir.
  • FIG. 13 is a schematic of a the process stages.
  • FIGS. 14A and 14B are photographs of dispensing of droplets.
  • material accumulates on the tip of the deposition head as a nano-liter droplet.
  • Photographs of the accumulating droplet can be analyzed for mixing.
  • Homogenous droplets present red coloring.
  • the heterogeneous droplets present both red and green coloring. The two colors are presented on discrete sides of the droplet.
  • the success of droplet formability requires that the alginate gel in the cross-linking bath before dispersing in the bath due to the inertial force of the printing process.
  • the inertial forces of the flowing fluid degrades the droplet' s spherical structure before gelation if the cross-linking concentration is low.
  • Aqueous alginate solution will flow until sufficient time in contact with cross-linking solution causes gelation.
  • printed droplet solidity e.g., torus or sphere
  • Cross-linking time is a function of the alginate and calcium chloride concentration.
  • Cross-linking concentration can be controlled between about 0.2 g/mL and 1.0 g/mL calcium chloride in distilled water.
  • Alginate concentration can be constant 0.5% (w/v) throughout the study.
  • the length of time required for cross-linking can be inversely related to the concentration of the crossing solution, for a given concentration of alginate.
  • Time lapse photography of the droplet dispensing into the cross-linking pool presents the effect of cross-linking solution concentration on formability of droplets and the ability to produce torus structures.
  • FIGS. 16A and 16B depicts alginate dispersed into 0.2 g/mL calcium chloride. The printed material flows away from the deposition point and degrades any printed structure. Further perturbation of the cross-linking reservoir will cause the droplet to twist and lose all recognizable shape.
  • FIG. 17 provides an analytical model for printing heterogeneous nano-liter droplets.
  • the novel synchronized multi-material bioprinter integrates microfluidic techniques with 3D cell printing to package multiple cell-laden materials and cross-linking solutions along a tool path or as a nanoliter droplet.
  • Biomimetic assembly to support cell viability in vitro and solicit paracrine/autocrine signaling between cells presents methodological progress to guide the cell aggregate to perform tissue-level function; with application to tissue engineering and general built biological constructs.
  • Bioprinting is an enabling technology to engineer built biological systems. Interfacing biology and architecture in built biological systems with reproducibility and engineering process control requires advanced manufacturing.
  • Bioprinting is a computer aided manufacturing method with process control over (1) macro-scale (10 "3 -10 _1 m) architecture and (2) micro-scale (10 ⁇ 4 -10 ⁇ 5 m) heterogeneous packaging of components and internal features.
  • Macro- scale architecture is critical if the built biological system is designed to physically fit into a larger system.
  • Micro-scale heterogeneous packaging of organisms and support artifacts is critical to the function of the built biological system. Additional artifacts heterogeneously packed with the biology during printing spatial -temporally effect the built system' s mechanics, physics, and chemistry.
  • Artifact candidates include load bearing structural elements, open porous networks for diffusion, functionalized or magnetic nanoparticles, and piezoelectric/conductive
  • the methods described herein can be readily implemented in software that can be stored in computer-readable media for execution by a computer processor.
  • the computer- readable media can be volatile memory (e.g., random access memory and the like) non-volatile memory (e.g., read-only memory, hard disks, floppy disks, magnetic tape, optical discs, paper tape, punch cards, and the like).
  • the methods described herein can be implemented in computer hardware such as an application-specific integrated circuit (ASIC).
  • ASIC application-specific integrated circuit

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  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Mechanical Engineering (AREA)
  • Materials For Medical Uses (AREA)

Abstract

Un aspect de l'invention concerne un procédé de production d'un filament hétérogène. Le procédé comprend l'application d'une pression à une pluralité de composants de filament pour déplacer les composants de filament dans un canal de sortie commun. Un autre aspect de l'invention concerne un procédé de fabrication d'un échafaudage. Le procédé comprend la production d'une pluralité de filaments selon les procédés de l'invention. Un autre aspect de l'invention concerne un échafaudage produit selon les procédés de l'invention. Un autre aspect de la présente invention concerne un filament hétérogène comprenant une pluralité de composants de filament adjacents. Un autre aspect de l'invention concerne un procédé de production d'une gouttelette. Le procédé comprend : le positionnement d'une pointe d'impression à proximité d'un liquide de réticulation ; l'application d'une pression à un ou plusieurs liquides pour produire une gouttelette s'étendant au-delà de l'extérieur de la pointe d'impression ; et la mise en contact de la gouttelette avec le liquide de réticulation, de manière à produire une gouttelette.
PCT/US2016/042032 2015-07-27 2016-07-13 Filaments hétérogènes, procédés de production de ceux-ci, échafaudages, procédés de production de ceux-ci, gouttelettes, et procédés de production de celles-ci Ceased WO2017019300A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2993779A CA2993779A1 (fr) 2015-07-27 2016-07-13 Filaments heterogenes, procedes de production de ceux-ci, echafaudages, procedes de production de ceux-ci, gouttelettes, et procedes de production de celles-ci
US15/745,299 US20180209069A1 (en) 2015-07-27 2016-07-13 Heterogeneous filaments,methods of producing the same, scaffolds, methods of producing the same, droplets, and methods of producing the same
CN201680043828.2A CN107847644A (zh) 2015-07-27 2016-07-13 非均质细丝和产生其的方法，支架和产生其的方法，微滴和产生其的方法
EP16831021.7A EP3328456A4 (fr) 2015-07-27 2016-07-13 Filaments hétérogènes, procédés de production de ceux-ci, échafaudages, procédés de production de ceux-ci, gouttelettes, et procédés de production de celles-ci

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562197329P 2015-07-27 2015-07-27
US62/197,329 2015-07-27

Publications (1)

Publication Number Publication Date
WO2017019300A1 true WO2017019300A1 (fr) 2017-02-02

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PCT/US2016/042032 Ceased WO2017019300A1 (fr) 2015-07-27 2016-07-13 Filaments hétérogènes, procédés de production de ceux-ci, échafaudages, procédés de production de ceux-ci, gouttelettes, et procédés de production de celles-ci

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US (1) US20180209069A1 (fr)
EP (1) EP3328456A4 (fr)
CN (1) CN107847644A (fr)
CA (1) CA2993779A1 (fr)
WO (1) WO2017019300A1 (fr)

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WO2017143355A3 (fr) * 2016-01-20 2017-11-09 Sunp Biotech, Llc Tête de fabrication de cellules/substances biologiques à entraînement par vis direct destinée à l'assemblage de constructions de tissu en 3d
WO2018165761A1 (fr) * 2017-03-15 2018-09-20 Aspect Biosystems Ltd. Systèmes et procédés d'impression d'une structure fibreuse
WO2020056517A1 (fr) * 2018-09-19 2020-03-26 Aspect Biosystems Ltd. Systèmes et procédés d'impression d'une fibre à noyau

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CN112708949B (zh) * 2020-12-23 2022-07-22 广西大学 一种基于微流体组装高强度纳米纤维素纤维的制备方法
US20240165879A1 (en) * 2021-03-26 2024-05-23 University Of Connecticut Multi-material bioprinting

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US20150018968A1 (en) * 2005-02-18 2015-01-15 Drexel University Method for creating an internal transport system within tissue scaffolds using computer-aided tissue engineering
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Cited By (15)

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WO2017143355A3 (fr) * 2016-01-20 2017-11-09 Sunp Biotech, Llc Tête de fabrication de cellules/substances biologiques à entraînement par vis direct destinée à l'assemblage de constructions de tissu en 3d
EP3595872A4 (fr) * 2017-03-15 2021-05-05 Aspect Biosystems Ltd. Systèmes et procédés d'impression d'une structure fibreuse
JP7144434B2 (ja) 2017-03-15 2022-09-29 アスペクト バイオシステムズ リミティド 繊維構造をプリントするためのシステム及び方法
CN110891764A (zh) * 2017-03-15 2020-03-17 安斯百克特生物系统公司 用于打印纤维结构的系统和方法
IL269149B2 (en) * 2017-03-15 2024-04-01 Aspect Biosystems Ltd Systems and methods for printing fibrous structure
JP2020510561A (ja) * 2017-03-15 2020-04-09 アスペクト バイオシステムズ リミティド 繊維構造をプリントするためのシステム及び方法
WO2018165761A1 (fr) * 2017-03-15 2018-09-20 Aspect Biosystems Ltd. Systèmes et procédés d'impression d'une structure fibreuse
CN110891764B (zh) * 2017-03-15 2022-04-26 安斯百克特生物系统公司 用于打印纤维结构的系统和方法
KR20190124281A (ko) * 2017-03-15 2019-11-04 애스펙트 바이오시스템즈 리미티드 섬유 구조물을 인쇄하기 위한 시스템 및 방법
KR102470715B1 (ko) * 2017-03-15 2022-11-24 애스펙트 바이오시스템즈 리미티드 섬유 구조물을 인쇄하기 위한 시스템 및 방법
US11724450B2 (en) 2017-03-15 2023-08-15 Aspect Biosystems Ltd. Systems and methods for printing a fiber structure
IL269149B1 (en) * 2017-03-15 2023-12-01 Aspect Biosystems Ltd Systems and methods for printing a fibrous structure
AU2018233180B2 (en) * 2017-03-15 2023-12-14 Aspect Biosystems Ltd. Systems and methods for printing a fiber structure
WO2020056517A1 (fr) * 2018-09-19 2020-03-26 Aspect Biosystems Ltd. Systèmes et procédés d'impression d'une fibre à noyau
US12186982B2 (en) 2018-09-19 2025-01-07 Aspect Biosystems Ltd. Systems and methods for printing a core fiber

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Publication number Publication date
CN107847644A (zh) 2018-03-27
CA2993779A1 (fr) 2017-02-02
EP3328456A4 (fr) 2019-06-26
US20180209069A1 (en) 2018-07-26
EP3328456A1 (fr) 2018-06-06

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