WO2017007140A1 - Oral composition for preventing and treating periodontitis, comprising mulberry root bark extract and winged spindle extract as active ingredients - Google Patents

Abstract

The present invention relates to an oral composition for preventing and treating periodontitis, comprising a mulberry root bark extract and a winged spindle extract as active ingredients and, more particularly, to an oral composition which comprises a mulberry root bark extract and a winged spindle extract as active ingredients and shows an excellent effect in the prevention, alleviation and treatment of periodontitis. The present invention provides an oral composition or a health functional food showing an excellent effect in the prevention, alleviation and treatment of periodontitis.

Description

Oral composition for the prevention and treatment of periodontitis, including the extract of cedar extract and arrowwood extract

The present invention relates to an oral composition for the prevention and treatment of periodontal disease comprising cedar extract and arrowwood extract as an active ingredient, more specifically, the extract of cedar extract and arrowwood extract showing an excellent effect on the prevention, improvement and treatment of periodontitis It relates to an oral composition containing as a component.

Various kinds of microorganisms live in the oral cavity of humans, and oral microorganisms causing oral diseases are known to exist in the plague, which is a bacterial membrane formed between teeth and gums.

The oral microorganisms produce toxins in the plaque, and the toxins they produce are periodontal disease when they invade the periodontal tissue and cause inflammation of the gingiva, destruction of alveolar bone, and infection, and when these bacteria produce acid, It can also be a cause.

As described above, microorganisms distributed in the oral cavity cause molar tooth and periodontal disease, and some of these microorganisms are known as opportunistic pathogens and have the potential to develop diseases.

Several species are known as pathogenic microorganisms that occur in the mouth and cause periodontal disease. Among them, Streptococcus mutans and Porphyromonas porphyromonas gingivalis ) is known to be the main pathogen causing periodontal disease.

Periodontitis is complicated and has a variety of symptoms. One of the main causes of periodontitis is caused by microorganisms in the oral cavity. They primarily produce insoluble glucan using sugars and the like and attach to the tooth surface like various cells to form tartar. Tooth decay and periodontitis are caused by these tartars.

Therefore, the most effective way to prevent periodontitis can be achieved by using a substance that inhibits the activity of these microorganisms and improves the blood flow of the inflamed gums. In other words, proper oral cleanliness is important for the prevention of periodontitis, but a composition for oral cavity in which various drugs are formulated for the purpose of prophylactic treatment has been proposed.

In particular, antibiotics such as penicillin, erythromycin, and tetracycline among the drugs used for the prophylactic treatment of periodontitis are effective in inhibiting sterilization and growth, but have a large side effect such as the occurrence of resistant bacteria. In addition, antimicrobial agents such as chlorhexidine have been shown to be effective in inhibiting tooth decay, but are highly toxic.

Therefore, in recent years, in order to solve the above problems, oral compositions having excellent oral pharmacological action and stability have been developed.

Related prior art, Korean Patent Publication No. 10-2012-0022423 "composition for oral hygiene comprising the seaweed extract as an active ingredient" and Korean Patent Publication No. 10-2008-0113504 "Ginseng Berry Extract as an active ingredient Containing oral bacterium inhibiting composition ".

Morus bark is Morus alba L.) or the root bark of the same plant has long been used in herbal medicine as an anti-inflammatory diuretic, laxative and antitussive expectorant.

Recently, various ingredients contained in these baekryepi is gradually revealed and its pharmacological effects have been identified. In particular, blood pressure lowering, blood sugar lowering and analgesic are widely known.

The winged spindle is a deciduous shrub to the rime vine, and is distributed in Korea, Japan, Sakhalin and China. Young leaves are herbs and the wings of branches are called earheads. The leaves contain epipriedelanol, priedelin, quercetin and dulcitol. Seed oil contains saturated fatty acids (20%), oleic acid, linoleic acid, linolenic acid, capric acid and benzoic acid. Oriental medicine is used for hemostasis, gore blood, pain.

Therefore, the present inventors have completed the present invention by confirming the efficacy using the baekbaekpi and arrowwood exhibiting the above-described efficacy in order to develop a drug exhibiting an excellent antimicrobial effect against the periodontitis causing bacteria.

It is an object of the present invention to provide an oral composition for preventing and treating periodontal disease, which comprises an extract of cedar and arrowwood as an active ingredient.

Technical problems to be achieved by the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned above may be clearly understood by those skilled in the art from the following description. There will be.

Oral composition for preventing and treating periodontal disease or periodontal disease prevention and improvement of the health functional food of the present invention to achieve the above object, including the extract of cedar and arrow extract as an active ingredient, showing the growth inhibitory activity of periodontitis-inducing bacteria Is characteristic.

The periodontitis causing bacteria is Porphyromonas ( Phyphyromonas) gingivalis).

The cedar extract and arrowwood extract is characterized by consisting of 1: 1 weight ratio.

The lettuce extract or arrow extract is characterized in that extracted with ethanol.

The oral composition is characterized by having any one or more formulations selected from toothpaste, chewing gum, mouthwash, mouthwash and mouth spray.

The dietary supplement is characterized by having any one or more formulations selected from powders, granules, tablets, capsules or beverages.

According to the present invention, an oral composition or a health functional food is provided which includes an extract of cedar extract and arrowwood extract as an active ingredient, and which exhibits an excellent effect on the prevention, improvement and treatment of periodontitis. Thus, as compared with the conventional toothpaste, it is possible to obtain the effect of preventing or treating periodontitis with the improvement of the stability of the oral drug active ingredient.

1 is a graph showing the antimicrobial activity of lettuce extract.

Figure 2 is a graph showing the antimicrobial activity of arrowwood extract.

Figure 3 is a graph showing the antimicrobial activity of the mixed extract (10 ppm) consisting of cedar extract (5 ppm) + arrow extract (5 ppm).

Figure 4 is a graph showing the antimicrobial activity of the mixed extract (50 ppm) consisting of lettuce extract (25 ppm) + arrowwood extract (25 ppm).

Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

The present invention provides an oral composition for preventing and treating periodontal disease, including the extract of cedar extract and arrowwood extract as an active ingredient.

At this time, the baekpipi extract or arrowwood extract is dried by extracting the baekpipi or arrowwood, respectively, 5 to 15 times the solvent of each weight (g), preferably water, lower alcohol of C1 to C4 or a mixed solvent thereof Extracts soluble in a solvent selected from, more preferably 1 to 10 times, preferably 3 to 10 hours at 10 to 50 ° C, preferably 20 to 30 ° C, preferably 3 to 36 hours with ethanol Can be obtained by repeated extraction five times.

When the extract is used as an active ingredient and the extract of the cedar extract is used as an active ingredient, the extract of the cedar extract should be contained in a high concentration in the antibacterial activity against periodontitis-inducing bacteria, the arrow extract extract has antibacterial activity even at 100ppm concentration Not shown. In order to produce a composition that can be commercialized by showing the antimicrobial activity against the periodontitis-inducing bacteria of the present invention even at low concentrations of extracts, it is preferable to use the mixture of the lettuce extract and the arrowwood extract, oral compared to conventional toothpaste. In addition to improving the stability of the active ingredient will be able to obtain the prevention and treatment of periodontitis.

At this time, in order to obtain the best growth inhibitory activity of the periodontitis-inducing bacteria of the present invention, it is good to use the extract from the cedar extract and the arrowwood extract in a 1: 1 weight ratio and use it as an active ingredient for oral composition for the prevention and treatment of periodontitis. Among periodontitis-causing bacteria, Porphyromonas gingivalis ) is characterized by showing antimicrobial activity.

As described above, the oral composition containing the extract of the present invention may be prepared by using a formulation such as toothpaste, chewing gum, mouthwash, mouthwash and mouth spray, but is not limited thereto.

Toothpaste of the present invention comprises a composition selected from the group consisting of abrasives, binders, moisturizers, active ingredients, foaming agents, sweeteners, flavoring agents.

Abrasives include aluminum hydroxide, silicic anhydride, aluminum silicate, dibasic calcium phosphate, dihydrate and anhydride, tricalcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, tribasic magnesium phosphate, magnesium carbonate and calcium sulfate , Polymethyl methacrylate, and other synthetic resins may be used in combination of one or two or more of them in an amount of about 20-90% of the total composition (20-60% in the case of paste toothpaste).

As a caking agent, synthetic polymers, such as a garginine, various cellulose derivatives for thickening, gums, such as a xanthan gum and a tragacanth gum, polyvinyl alcohol, sodium polyacrylate, a polyacrylic acid / maleic acid copolymer, and carboxyvinyl polymer In organic binders, such as derivatives, and inorganic binders, such as a silica and a laponite, 1 type, or 2 or more types can be mix | blended in the quantity of about 0.3-5% of the whole composition.

Paste and liquid oral compositions in dentifrices include sorbitol, glycerin, ethylene glycol, propylene glycol, polyether glycol, polypropylene glycol, and the like as moisturizing agents in the preparation of about 10-70% of the total composition. It can be used in combination.

On the other hand, oral compositions containing the extract of the present invention include menthol (Mentol), anetol (Anetol), eugenol (Eugenol), limonene (Limonene), Ocimene, n-dodecyl alcohol (n-Dodecy Alcohol, Citronenol, Alpha-Terpineol, Methyl salycilate, Methyl Acetate, Citroneryl Acetate, Cineol, Linalol, Ethyl Linalool, Vanillin, Thymol, Spearamint Oil, Sage Oil, Rosemary Oil, Cinnamon Oil, etc. The agents may be used alone or in combination in amounts of 0.1-10%, preferably 0.5-5%, of the total composition.

In addition, sweeteners such as sodium saccharin, stevioside, aspartame, D-Xylose and the like can be used in combination in an amount of 0-1%, preferably 0.01-0.5% of the total composition.

In addition, oral compositions of the present invention include trichloric acid, chlorohexidine, benzetonium chloride, benzalkonium chloride, cationic fungicides such as cetylpyridium chloride, alkali metal monofluoro such as sodium monofluorophosphate and potassium monophosphate, etc. Fluorides such as phosphate, sodium fluoride and tin fluoride, tranexamic acid, epsilon amino capronic acid, aluminum chlorohydroxy allantoin, dihydro cholestanol, cletylithic acid, glycerophosphate, sodium chloride, and water-soluble inorganic phosphate compounds And one or two or more of various potent ingredients such as ascorbic acid (sodium), dl-a-tocopherol acetate, xylitol, pyridoxine hydrochloride and polyvinyl pyrrolidone can be used in combination.

In addition, the surfactant used as the foaming component in the oral composition of the present invention may be used anionic surfactants, nonionic surfactants and / or zwitterionic surfactants. Here, the anionic surfactants include sodium lauryl sulfate, sodium alkyl sulfates such as myristyl sulfate, sodium N-Lauroyl sarcosinate, sodium N-miristyl sarcosinate, and the like. Sodium N-acyl sarcosinate may be used, and non-ionic surfactants include sugar-derived fatty acid esters such as sugar fatty acid esters, maltose fatty acid esters, lactose fatty acid esters, and polyesters. Oxy ethylene hardened castor oil, polyoxyethylene higher alcohol ether, polyoxy ethylene polyoxy propylene copolymer, polyoxy ethylene polypropylene fatty acid ester and the like can be used. Zwitterionic surfactants include N-alkyl diaminoethylglycine, such as N-lauryl di aminoethylglycine, N-myristyl diaminoethylglycine, N-alkyl-N-carboxy methyl ammonium betaine, 2-alkyl-1 -Hydroxy ethyl imidazoline betaine sodium, etc. can be used. In this case, anionic surfactants are mainly used as the foaming component, and sodium alkyl sulfates such as sodium lauryl sulfate are most widely used. Moreover, these surfactant is used individually by 1 type or in mixture of 2 or more types, and the compounding quantity normally uses 0.01-0.5% of a whole composition, More preferably, 0.05-0.3%.

In addition, the present invention provides a health functional food for the prevention and improvement of periodontitis containing the extract as an active ingredient.

Health functional foods including the extract of the present invention can be used in a variety of drugs, foods and drinks for the prevention and improvement of periodontitis. Foods to which the extract of the present invention may be added include, for example, various foods, beverages, gums, teas, vitamin complexes, health supplements, and the like, and may be used in the form of powders, granules, tablets, capsules, or beverages. have.

Since the extract itself of the present invention has little toxicity and side effects, it is a drug that can be used safely even when taken for long periods of time.

Thus, the extract of the present invention may be added to food or beverage for the purpose of preventing and improving periodontitis. At this time, the amount of the extract in the food or beverage is generally the health food composition of the present invention can be added to 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 10 g, preferably based on 100 ml It can be added at a ratio of 0.3 to 1 g.

The health beverage composition of the present invention, in addition to containing the extract or compound as an essential ingredient in the indicated ratio, there is no particular limitation on the liquid component, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. have. Examples of the above-mentioned natural carbohydrates are conventional monosaccharides such as disaccharides such as glucose and fructose, such as maltose, sucrose and the like, and polysaccharides such as dextrin, cyclodextrin and the like. Sugars and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the health functional food of the present invention may contain natural fruit juice and fruit flesh for producing fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but these Examples and Experimental Examples are for illustrative purposes only and are not intended to limit the protection scope of the present invention.

Example 1 Preparation of a Extract Extract Composed of Cedar Extract + Arrow Extract

end. Cereal Extract Extract

After washing the baekbaekpi with purified water, it was naturally dried at room temperature for 7 days.

After drying, the powder was ground in a grinder, and then 1 kg of the baekbaekpi powder was placed in 10 L of 70% ethanol and extracted under reflux for 3 hours at room temperature. The extract was repeated three times.

The extract was filtered to recover only the extract filtrate, and then distilled under reduced pressure to prepare a liquid extract extract (receiving concentration: 10000 ppm).

At this time, the prepared lettuce extract was diluted by 10 times step by step using sterile distilled water was prepared by concentration (0.1, 1, 10, 100, 1000, 10000 ppm).

B, arrow extract manufacture

The arrow branches were washed with purified water and then naturally dried at room temperature for 7 days.

After drying, the powder was ground in a grinder, and then 1 kg of the arrowwood powder was placed in 10 l of 70% ethanol, and extracted under reflux for 3 hours at room temperature. The extract was repeated three times.

The extract was filtered to recover only the extract filtrate and distilled under reduced pressure to prepare a liquid arrow extract (receiving concentration: 10000 ppm).

At this time, the manufactured arrow extract was diluted by 10 times step by step using sterile distilled water was prepared by concentration (0.1, 1, 10, 100, 1000, 10000 ppm).

All. Mixed Extract Preparation

Above a. The extract of baekbaekpi prepared in the above b. Prepared by the same concentration of arrowwood extract prepared in the mixed extract was prepared in a 1: 1 weight ratio.

Experimental Example 1 Antibacterial Activity

Minic inhibitory concentration (MIC) tests were performed to determine the antimicrobial activity of periodontal fungi of lettuce extract, arrow extract and mixed extracts.

1. Strains to be tested

As the strain used, a strain of periodontal-induced bacterium Popiromonas jinjibaris, which was distributed by the ATCC American Microbial Resource Center (ATCC BAA-1703 ^™ ), was used.

2. Test method

1) Strain Culture Conditions

Porphyromonas jinjibaris, the periodontitis-inducing bacterium gingivalis) strains were inoculated in Brain heart infusion broth medium containing 5ug / ml hemin and 1ug / ml vitamin K, and then cultured at 37 ° C.

2) Treatment of Extract

① Mix the mixed solution by mixing 0.9 ml of sterilized liquid medium, 0.1 ml (10%, V / V), and 1 ml of bacterial culture solution at a concentration of 10 ⁷ cfu / ml so that the final mixture is 1 ml. Prepared.

② For extract, the diluted solution was diluted 10 times step by step using the stock solution and sterile distilled water.

③ Mixture treatment of baekbaekpi extract and arrowwood extract put each extract 0.05 ㎖, if the extract is not treated 0.1 sterile distilled water to be treated.

3) Culture method

The test tube containing the mixed solution of ①, including the bacterial culture solution, the extract and the sterilized liquid medium, was incubated at 37 ° C. for 30 hours.

Antibiotic determination was carried out every 3 hours of culture, 0.1 ml of the mixed solution of each treatment was measured using a 96 well palte to measure the change in absorbance due to bacterial growth at 600 nm.

2. Experimental Results

1) Antimicrobial Activity of Extracts

1 is Porphyromonas gingivalis ) As a test for the antimicrobial activity of the extracts from the extracts of the lettuce, it was confirmed that the antimicrobial activity was almost absent at low concentrations of 10 ppm, and slightly weaker at 25 ppm.

2) Antimicrobial Activity of Arrowwood Extract

Figure 2 is Porphyromonas jinjibaris gingivalis ) As a test for the antimicrobial activity of the arrowwood extract in the strain, it was confirmed that almost no antimicrobial activity at low concentrations of 10 ppm, 25 ppm, 50 ppm.

3) Antimicrobial Activity of Mixed Extract Composed of Morus Extract + Arrow Extract

3 and 4 in the foreskin Monastir seriously Baris (Porphyromonas gingivalis ) As a test for the antimicrobial activity of the mixed extract consisting of lettuce extract + arrowroot extract against the strain, 10 ppm (Fig. 3) and 50 ppm (Fig. 4) of the mixed extract was confirmed the antimicrobial activity. .

As shown in FIG. 3, low activity of 5 ppm of arrowwood extract (-■-) showed little activity, and almost no activity of 5 ppm of cedar extract (-▲-). It was. In addition, it was confirmed that the active ingredient of the present invention 1: 1 mixed extract 10 ppm (5 ppm each of the fungus extract and arrowwood extract, Example 1) showed almost no antibacterial activity (-◆-).

As shown in Figure 4, when treated with 25 ppm alone arrow extract (-■-) showed almost no activity, and when treated with 25 ppm single leaf extract (-▲-) showed a poor antimicrobial activity However, while the present invention does not reach the desired antimicrobial activity, in the case of 50 ppm (25 ppm each of cedar extract and arrowwood extract, Example 1), which is an active ingredient of the present invention, in the case of periodontitis-inducing bacteria As a potent inhibitory activity against Porphyromonas gingivalis ( Porphyromonas gingivalis ), 1: 1 mixed extract (25 ppm each of the extracts of cedar and arrowwood extract, Example 1), which is an active ingredient of the present invention, was more antibacterial than a single extract. It was judged to have a synergistic effect on activity (-◆-).

Therefore, when using the composition for preventing and treating periodontitis prepared according to the present invention, it is expected to exhibit excellent antimicrobial activity against the strain causing periodontitis, and to obtain the effect of preventing and treating periodontitis. As it can be used as a material such as, it is possible to expect an increase in added value.

<Example 1: Preparation of toothpaste>

The components belonging to a of Table 1 were added to the production unit at a temperature of 70 to 75 ° C., and stirred for 20 minutes to completely dissolve the powder and then cooled to room temperature.

Subsequently, as a dissolution process of the moisturizer, sweetener and useful components, the components belonging to b including the carrier obtained in Example 1 were added to the production unit at 25 to 30 ° C., followed by stirring for 15 minutes to dissolve.

Next, as a powder and viscous mixing process, the components belonging to c were mixed at 25 to 35 ° C. and added in stages. The next process was a foaming agent mixing and primary degassing process, in which the components belonging to d were added to complete the primary defoaming during the 40 minute process at 25 to 35 ° C. The final process is a fragrance mixing process, to which toothpaste was prepared by adding a component belonging to e and degassing at 25 to 35 ° C.

The composition was according to Table 1 below.

Purpose of Mixing Ingredient Name standard amount unit Remarks solvent Purified water pharmacopoeia Quantity a Mechanism Dissorbitol solution pharmacopoeia 33.00 g Mechanism Propylene glycol Food 5.10 g Mechanism Concentrated glycerin pharmacopoeia 8.25 g chief ingredient Precipitated Calcium Carbonate pharmacopoeia 30.00 g b chief ingredient Amino acid capronic acid pharmacopoeia 0.01 g chief ingredient Calcium dihydrogen phosphate pharmacopoeia 0.01 g Thickener Silicon dioxide Food 18.84 g antiseptic Sodium benzoate Food 0.18 g Sweetener Green Tea Extract pharmacopoeia 0.01 g Sweetener Zaccarin Sodium Food 0.01 g Sweetener Xylitol Food 0.01 g Sweetener Chitosan Food 0.01 g Sweetener Propolis Extract Annex 0.01 g Sweetener Mixture Extract of Example 1 0.01 g Mechanism Polyethylene glycol 1500 pharmacopoeia 0.48 g c Viscosity Modifier Carboxymethyl Cellulose Sodium Food 0.72 g Foam Sodium Lauryl Sulfate pharmacopoeia 2.44 g d Flavor DL-menthol Food Quantity g Flavor Peppermint Rain, blood Quantity g e 100g total

The present invention has been described with reference to preferred embodiments, and those skilled in the art to which the present invention pertains to the detailed description of the present invention and other forms of embodiments within the essential technical scope of the present invention. Could be. Here, the essential technical scope of the present invention is shown in the claims, and all differences within the equivalent range will be construed as being included in the present invention.

Claims (12)

Including cedar extract and arrow extract as active ingredients, Characterized by showing the growth inhibitory activity of periodontal causative bacteria, Oral composition for preventing and treating periodontal disease. The method of claim 1, The periodontitis causing bacteria is porphyromonas jinjibaris ( Porphyromonas gingivalis ), characterized in that Oral composition for preventing and treating periodontal disease. The method of claim 1, The cedar extract and the arrowwood extract is characterized in that the weight ratio of 1: 1, Oral composition for preventing and treating periodontal disease. The method of claim 1, The extract extract is characterized in that extracted with ethanol, Oral composition for preventing and treating periodontal disease. The method of claim 1, The arrow extract is characterized in that extracted with ethanol, Oral composition for preventing and treating periodontal disease. The method of claim 1, The oral composition is characterized in that it has any one or more formulations selected from toothpaste, chewing gum, mouthwash, mouthwash and mouth spray, Oral composition for preventing and treating periodontal disease. It contains cedar extract and arrow extract as an active ingredient, Characterized by showing the growth inhibitory activity of periodontitis-inducing bacteria, Health functional food for prevention and improvement of periodontal disease. The method of claim 7, wherein The periodontitis causing bacteria is characterized in that Porphyromonas gingivalis ( Porphyromonas gingivalis ), Health functional food for prevention and improvement of periodontal disease. The method of claim 7, wherein The cedar extract and the arrowwood extract is characterized in that the weight ratio of 1: 1, Health functional food for prevention and improvement of periodontal disease. The method of claim 7, wherein The extract extract is characterized in that extracted with ethanol, Health functional food for prevention and improvement of periodontal disease. The method of claim 7, wherein The arrow extract is characterized in that extracted with ethanol, Health functional food for prevention and improvement of periodontal disease. The method of claim 1, The health functional food is characterized in that it has any one or more formulations selected from powders, granules, tablets, capsules or beverages, Health functional food for prevention and improvement of periodontal disease.

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