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WO2017065708A1 - Dispositif automatique et en ligne de détection d'une mammite sous-clinique basé sur une diffusion optique et système d'échantillonnage de lait automatique comprenant ce dispositif - Google Patents

Dispositif automatique et en ligne de détection d'une mammite sous-clinique basé sur une diffusion optique et système d'échantillonnage de lait automatique comprenant ce dispositif Download PDF

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Publication number
WO2017065708A1
WO2017065708A1 PCT/TR2016/000134 TR2016000134W WO2017065708A1 WO 2017065708 A1 WO2017065708 A1 WO 2017065708A1 TR 2016000134 W TR2016000134 W TR 2016000134W WO 2017065708 A1 WO2017065708 A1 WO 2017065708A1
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WO
WIPO (PCT)
Prior art keywords
milk
channel
sampling system
subclinical mastitis
automatic sampling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/TR2016/000134
Other languages
English (en)
Inventor
Serhat SEVLI
Seyfullah YILMAZ
Hikmet KOCA
Vedat SAHIN
Giovanni Volpe
Sathyanarayana PALADUGU
Sabareesh KUNJIPALAYAM PALANISWAMY VELU
Alireza Moradi KOSHKABADI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nehir Biyoteknoloji Ar-Ge Hizm Dan Bils Paz San Tic Ltd Sti
Stc Elektronik Saglik Hiz San Tic Ltd Sti
Original Assignee
Nehir Biyoteknoloji Ar-Ge Hizm Dan Bils Paz San Tic Ltd Sti
Stc Elektronik Saglik Hiz San Tic Ltd Sti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nehir Biyoteknoloji Ar-Ge Hizm Dan Bils Paz San Tic Ltd Sti, Stc Elektronik Saglik Hiz San Tic Ltd Sti filed Critical Nehir Biyoteknoloji Ar-Ge Hizm Dan Bils Paz San Tic Ltd Sti
Publication of WO2017065708A1 publication Critical patent/WO2017065708A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01J—MANUFACTURE OF DAIRY PRODUCTS
    • A01J5/00—Milking machines or devices
    • A01J5/013—On-site detection of mastitis in milk
    • A01J5/0135—On-site detection of mastitis in milk by using light, e.g. light absorption or light transmission
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Definitions

  • the present invention relates to a subclinical mastitis detection device which carries out evaluation based on three mastitis criteria as SHS measurement and cell type characterization and number of bacteria by optical laser scattering analysis of cow's milk, uses very little amount of sample with the help of microfluidic channel, does not use any chemical having risk of contamination to milk; and an automatic milk sampling system comprising this device.
  • mastitis disease In milk farms, burning and inflammation occur inside udder tissue due to the fact that pathogenic (disease-causing) microorganisms contaminate to udders of cows and this disease is called as mastitis.
  • Mastitis disease has two subconditions. The first condition is called as clinical mastitis and it occurs due to diseases caused by some bacteria.
  • Clinical mastitis disease is understood via symptoms which can be seen by veterinarian and breeders externally such as redness of udder, the animal gets bad-tempered and does not allow contact to its udders, the milk becomes bloody and even the teat becomes blunt. Milk yield of cows getting clinical mastitis disease reduces in a certain amount and they must be definitely treated with antibiotics.
  • subclinical mastitis This condition is the one before the clinical mastitis and some pathogenic microorganisms can ensure that the animal remains only in subclinical mastitis condition as well.
  • Subclinical mastitis is also known as hidden mastitis and it has no symptom whereby a veterinarian or a breeder can understand occurrence of the disease with the naked eye upon microbe contamination to an animal's udder.
  • Counting the number of somatic cells in milk is the standard method being used at the present time in order to detect and follow-up subclinical mastitis in farms. If 200.000 or more cell number is found in 1 mL milk belonging to a single udder of a single animal, this animal is accepted as having mastitis. Although this method is globally accepted, it also has many disadvantages. The most important of these disadvantages is that: total number of cells in milk also increases in cases other than mastitis or there are unidentified sudden decreases in cell number in the event of sickness.
  • CMT California Mastitis Test
  • mastitis analysis devices examine health status of an animal by applying indirect method.
  • the said devices measure density of fat, protein, lactose and blood in milk by infrared spectrometry technology.
  • the International patent document no. WOOl 19170 discloses a device which detects mastitis or other udder disorders in milking animals.
  • analysis is enabled by means of two optical sensors disposed along a conduit and each including an optical emitter and a monitoring arrangement including a detector.
  • the vacuum pump of the device comprises waste water tank in the said invention.
  • LED is used as optical emitter and photodetectors are used as detector.
  • somatic cell count or neutrophil/macrophage ratios are not measured but lumpy particles, blood and inflammations are detected in the said device.
  • the United States patent document no. US6731100 discloses an online system which detects diseases by using chemical via somatic cell count in raw milk collected from milked animals. It is mentioned that the invention disclosed in the said document can be integrated to a milking system. In the invention, compressed air or pump is used in order that the sample proceeds in the channel. In the said device, the sample is focused by using a light source lens such as laser and monochromatic detector is used. In the device, use of fluorescent dye is mentioned for imaging sub cells and calculating only numbers of total somatic cell is disclosed. In addition, analysis is carried out inside the sample chamber in the device.
  • An objective of the present invention is to realize a subclinical mastitis detection device which has high detection sensitivity (95%), high accuracy (99%) and provides real-time alert for enterprises having particularly farming automation; and an automatic milk sampling system comprising this device
  • Another objective of the present invention is to realize a subclinical mastitis detection device which carries out evaluation based on three mastitis criteria as SHS (number of somatic cells) measurement and cell type characterization and number of bacteria by optical laser scattering analysis of milk, uses very little amount of sample with microfluidic chip technology, does not use any chemical having risk of contamination to milk; and an automatic milk sampling system comprising this device.
  • SHS number of somatic cells
  • Another objective of the present invention is to realize a device which has cheap operating cost, is easy-to-use with minimum farmer intervention; and an automatic milk sampling system comprising this device.
  • Another objective of the present invention is to realize a device which can be adapted or carried to any automatic milk sampling system.
  • Another objective of the present invention is to realize a device which can distinguish neutrophil and macrophage cells according to their optical scattering characteristics without fluorescent marking; and an automatic milk sampling system comprising this device.
  • Figure 1 is a schematic view of the inventive device.
  • Figure 2 is a schematic view of an automatic milk sampling system comprising the inventive device.
  • Waste channel The device (1) enabling to detect whether a milch cow has subclinical mastitis or not by analysing cow's milk comprises:
  • At least one microfluidic chip channel (2) which enables to take milk sample
  • at least one laser source (3) which sends laser beam onto the sample being included inside the channel (2)
  • the device (1) developed by the present invention laser beam is sent onto the sample being included inside the microfluidic chip channel (2) and it is ensured that the light which falls over the cells being included inside the sample is subjected to optical scattering. Due to the fact that different cells reflect light to directions at different angles with varying intensity, image of this reflecting light is received by the image reception unit (4) and it is ensured whether there is subclinical mastitis or not by making analysis of the sample upon being transmitted to the analysis unit (5).
  • the microfluidic chip channel (2) is a glass microfluidic channel or a capillary tube which performs hydrodynamic focusing.
  • the said channel (2) has a curved structure in order to facilitate image reception and milk cells are enabled to flow on a line consecutively in thereof.
  • the laser source (3) is a LED lamp.
  • the image reception unit (4) is a CCD camera, CMOS camera, array photodetector, line photodetector, dot photodetector or any sensor which senses optical data and turns it into electrical signal.
  • the device (1) comprises at least one lens (not shown in the figures) which is between the laser light source (3) and the image reception unit (4) and focuses the light to the channel (2).
  • the analysis unit (5) distinguishes neutrophil and macrophage cells according to their optical scattering characteristics in order to detect presence of subclinical mastitis and carries out the said detection by proportioning the cell numbers to one another. In another embodiment of the invention, the analysis unit (5) performs detection of subclinical mastitis by distinguishing normal milk cells and milk cells having subclinical mastitis according to their optical scattering characteristics without making discrimination of cell types.
  • the analysis unit (5) performs detection of subclinical mastitis by counting and proportioning lymphocyte and macrophage cells via optical scattering. In another embodiment of the invention, the analysis unit (5) performs detection of subclinical mastitis by counting and proportioning lymphocyte and neutrophil cells with one another via optical scattering.
  • the analysis unit (5) performs detection of subclinical mastitis by optical scattering and counting total somatic cell and counting total bacteria amount.
  • the device (1) comprises at least two image reception units (4) one of which is positioned in front of the laser beam source and receives forward scattering images and the other one thereof is positioned perpendicularly to the incidence angle of the scattering images' laser beam and receives side scattering images.
  • forward scattering (FSC) images give information about both cell dimension and cell structure whereas side scattering (SSC) images give additional information about the cell's internal structure and thus it is enabled to distinguish macrophages and neutrophils.
  • the inventive device (1) in one preferred embodiment of the invention, is included in an automatic sampling system (6).
  • the said automatic sampling system (6) comprises: at least one milking pump (7) which is connected to at least one udder of a cow and enables to milk;
  • At least one milk pump (10) which is located on the delivery channel (9) and enables to take milk sample;
  • At least one subclinical mastitis detection device (1) where the milk sample received from the milk pump (10) is delivered for subclinical mastitis detection.
  • the said system (6) comprises at least one valve (11) which is located on the delivery channel (9) and can be switched to on/off position. Upon the said valve (11) is opened, the milk included in the delivery channel (9) is drawn by means of the milk pump (10) and it is delivered to the channel (2) of the detection device (1).
  • the system (6) comprises at least one sensor (12) which is included in the delivery channel (9) and detects presence of milk and ensures that milk sample is taken with the help of the milk pump (10) by enabling to open the valve (11) after the presence of milk is detected.
  • the said sensor (12) is a motion sensor in one preferred embodiment of the invention.
  • the system (6) comprises at least one water channel (13) which is connected to the detection device (1) and provides water supply to the channel (2) included inside the device (1).
  • the sensor (12) ensures that water is also taken into the channel (2) of the device (1) at the same time by triggering the valve (15) included in the water channel (13) as well while enabling to take milk.
  • the system (6) comprises at least one waste channel (17) which is connected to the detection device (1) in order to remove the waste water and the milk sample included in the device (1) after the detection transaction is carried out.
  • the system (6) comprises at least one cleaning channel (not shown in the figures) which feeds cleaning fluid to the system (6) in order to clean the detection device (1) and the channels (2,8,9,13,17). It is possible to develop a great variety of embodiments of the inventive "A Subclinical Mastitis Detection Device (1) and an Automatic Milk Sampling System Comprising This Device (6)"; it cannot be limited to the examples disclosed herein; and it is essentially according to the claims.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Animal Husbandry (AREA)
  • Environmental Sciences (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un dispositif de détection d'une mammite sous-clinique qui effectue une évaluation en fonction de trois critères de mammite tels que la mesure SHS et la caractérisation du type de cellule et le nombre de bactéries par analyse de diffusion laser optique du lait de vache, qui utilise une très faible quantité d'échantillon au moyen de canaux microfluidiques, qui n'utilise aucun produit chimique présentant un risque de contamination pour le lait; et un système d'échantillonnage de lait automatique comprenant ce dispositif.
PCT/TR2016/000134 2015-10-12 2016-10-12 Dispositif automatique et en ligne de détection d'une mammite sous-clinique basé sur une diffusion optique et système d'échantillonnage de lait automatique comprenant ce dispositif Ceased WO2017065708A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2015/12622 2015-10-12
TR201512622 2015-10-12

Publications (1)

Publication Number Publication Date
WO2017065708A1 true WO2017065708A1 (fr) 2017-04-20

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PCT/TR2016/000134 Ceased WO2017065708A1 (fr) 2015-10-12 2016-10-12 Dispositif automatique et en ligne de détection d'une mammite sous-clinique basé sur une diffusion optique et système d'échantillonnage de lait automatique comprenant ce dispositif

Country Status (1)

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WO (1) WO2017065708A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111506881A (zh) * 2020-06-11 2020-08-07 中国农业大学 一种预测中国荷斯坦牛乳房炎发病风险的系统
US11243167B2 (en) 2017-08-18 2022-02-08 Somadetect Inc. Methods and systems for assessing a health state of a lactating mammal
CN114521106A (zh) * 2019-09-30 2022-05-20 利拉伐控股有限公司 自动挤奶系统和确定动物的健康状况的方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019170A1 (fr) 1999-09-15 2001-03-22 Delaval Holding Ab Dispositif de traite automatique d'animaux
US6731100B1 (en) 1997-05-05 2004-05-04 Chemometec A/S Method and a system for determination of somatic cells in milk
US20070289536A1 (en) * 2006-06-09 2007-12-20 Dairy Controls International, Inc. In-line apparatus and real-time method to determine milk characteristics
WO2008093344A1 (fr) * 2007-02-01 2008-08-07 E.N.G.S. Systems Ltd. Système pour détecter des particules dans un fluide lacté tel que le lait
WO2008146276A1 (fr) * 2007-05-31 2008-12-04 S.A.E Afikim Système et procédé pour analyser des fluides
WO2012052046A1 (fr) * 2010-10-18 2012-04-26 Foss Analytical A/S Procédé de détermination d'un degré d'infection
EP2916131A1 (fr) * 2014-03-05 2015-09-09 Amphasys AG Procédé et appareil pour déterminer une charge de cellule dans du lait

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6731100B1 (en) 1997-05-05 2004-05-04 Chemometec A/S Method and a system for determination of somatic cells in milk
WO2001019170A1 (fr) 1999-09-15 2001-03-22 Delaval Holding Ab Dispositif de traite automatique d'animaux
US20070289536A1 (en) * 2006-06-09 2007-12-20 Dairy Controls International, Inc. In-line apparatus and real-time method to determine milk characteristics
WO2008093344A1 (fr) * 2007-02-01 2008-08-07 E.N.G.S. Systems Ltd. Système pour détecter des particules dans un fluide lacté tel que le lait
WO2008146276A1 (fr) * 2007-05-31 2008-12-04 S.A.E Afikim Système et procédé pour analyser des fluides
WO2012052046A1 (fr) * 2010-10-18 2012-04-26 Foss Analytical A/S Procédé de détermination d'un degré d'infection
EP2916131A1 (fr) * 2014-03-05 2015-09-09 Amphasys AG Procédé et appareil pour déterminer une charge de cellule dans du lait

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11243167B2 (en) 2017-08-18 2022-02-08 Somadetect Inc. Methods and systems for assessing a health state of a lactating mammal
US11733160B2 (en) 2017-08-18 2023-08-22 Somadetect Inc. Methods and systems for assessing a health state of a lactating mammal
CN114521106A (zh) * 2019-09-30 2022-05-20 利拉伐控股有限公司 自动挤奶系统和确定动物的健康状况的方法
CN114521106B (zh) * 2019-09-30 2023-11-03 利拉伐控股有限公司 自动挤奶系统和确定动物的健康状况的方法
CN111506881A (zh) * 2020-06-11 2020-08-07 中国农业大学 一种预测中国荷斯坦牛乳房炎发病风险的系统

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