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WO2017059094A3 - Système et procédé de détection d'une maladie dans des fluides corporels - Google Patents

Système et procédé de détection d'une maladie dans des fluides corporels Download PDF

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Publication number
WO2017059094A3
WO2017059094A3 PCT/US2016/054483 US2016054483W WO2017059094A3 WO 2017059094 A3 WO2017059094 A3 WO 2017059094A3 US 2016054483 W US2016054483 W US 2016054483W WO 2017059094 A3 WO2017059094 A3 WO 2017059094A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
individual
hybridize
acid probe
specific nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2016/054483
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English (en)
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WO2017059094A2 (fr
Inventor
Adi Mashiach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
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Application filed by Individual filed Critical Individual
Publication of WO2017059094A2 publication Critical patent/WO2017059094A2/fr
Publication of WO2017059094A3 publication Critical patent/WO2017059094A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne, dans ses modes de réalisation, un système comportant : un dispositif microfluidique conçu pour recevoir un échantillon contenant au moins une molécule d'ARN provenant d'un sujet, le dispositif microfluidique comprenant une plaque de réaction qui contient une pluralité de zones individuelles, chaque zone individuelle comportant une sonde d'acide nucléique conçue pour s'hybrider à une molécule d'acide nucléique spécifique, chaque zone individuelle étant conçue pour générer une indication lorsque l'acide nucléique spécifique est lié à la sonde d'acide nucléique, au moins une zone individuelle à l'intérieur de la pluralité de zones individuelles comprenant une sonde d'acide nucléique unique conçue pour s'hybrider à une molécule d'acide nucléique spécifique comprenant un mARN, au moins une zone individuelle à l'intérieur de la pluralité de zones individuelles comprenant une sonde d'acide nucléique unique conçue pour s'hybrider à une molécule d'acide nucléique spécifique comprenant un ARN non codant ; un lecteur, conçu pour détecter l'indication générée et transmettre, en se basant sur la détection, un signal ; et un processeur programmé spécifiquement, pour recevoir le signal transmis, et exécuter une série de règles prédéfinies, et, sur la base des règles prédéfinies et du signal transmis, émettre un résultat.
PCT/US2016/054483 2015-09-29 2016-09-29 Système et procédé de détection d'une maladie dans des fluides corporels Ceased WO2017059094A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562234467P 2015-09-29 2015-09-29
US62/234,467 2015-09-29

Publications (2)

Publication Number Publication Date
WO2017059094A2 WO2017059094A2 (fr) 2017-04-06
WO2017059094A3 true WO2017059094A3 (fr) 2017-05-11

Family

ID=58427861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/054483 Ceased WO2017059094A2 (fr) 2015-09-29 2016-09-29 Système et procédé de détection d'une maladie dans des fluides corporels

Country Status (1)

Country Link
WO (1) WO2017059094A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11788120B2 (en) 2017-11-27 2023-10-17 The Trustees Of Columbia University In The City Of New York RNA printing and sequencing devices, methods, and systems
JP7414237B2 (ja) 2018-02-13 2024-01-16 東レ株式会社 認知症の検出のためのキット又はデバイス及び方法
JP7324835B2 (ja) * 2018-08-28 2023-08-10 エフ． ホフマン－ラ ロシュ アーゲー ルテニウム含有電極を備えるナノポアシーケンシングデバイス
CN113265447B (zh) * 2021-04-14 2022-06-21 军事科学院军事医学研究院环境医学与作业医学研究所 一种用于检测肌酸激酶同工酶的滚环扩增-金四面体比色检测方法和试剂盒

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010026916A1 (en) * 1998-10-30 2001-10-04 Stephen Ginsberg Diagnostic methods for alzheimer's disease by detection of multiple mrnas
US20090137415A1 (en) * 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
US20110312641A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Microfluidic device with sample inlet and probe hybridization section
WO2012080753A1 (fr) * 2010-12-16 2012-06-21 Genetic Analysis As Diagnostic de la maladie de crohn
US20130157272A1 (en) * 2011-09-13 2013-06-20 Alex Adai Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
US20150119280A1 (en) * 2013-10-28 2015-04-30 Massachusetts Institute Of Technology Hydrogel Microstructures with Immiscible Fluid Isolation for Small Reaction Volumes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010026916A1 (en) * 1998-10-30 2001-10-04 Stephen Ginsberg Diagnostic methods for alzheimer's disease by detection of multiple mrnas
US20090137415A1 (en) * 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
US20110312641A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Microfluidic device with sample inlet and probe hybridization section
WO2012080753A1 (fr) * 2010-12-16 2012-06-21 Genetic Analysis As Diagnostic de la maladie de crohn
US20130157272A1 (en) * 2011-09-13 2013-06-20 Alex Adai Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
US20150119280A1 (en) * 2013-10-28 2015-04-30 Massachusetts Institute Of Technology Hydrogel Microstructures with Immiscible Fluid Isolation for Small Reaction Volumes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MIOTKE, L ET AL.: "Novel Signal-Enhancing Approaches for Optical Detection of Nucleic Acids- Going beyond Target Amplification.", CHEMOSENSORS., vol. 3, no. 3, 23 September 2015 (2015-09-23), pages 224 - 240, XP055381503 *

Also Published As

Publication number Publication date
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