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WO2017051437A3 - A nucleotide sequence encoding 7-dehydrocholesterol reductase, recombinant constructs and recombinant organisms comprising said sequence - Google Patents

A nucleotide sequence encoding 7-dehydrocholesterol reductase, recombinant constructs and recombinant organisms comprising said sequence Download PDF

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Publication number
WO2017051437A3
WO2017051437A3 PCT/IN2016/050323 IN2016050323W WO2017051437A3 WO 2017051437 A3 WO2017051437 A3 WO 2017051437A3 IN 2016050323 W IN2016050323 W IN 2016050323W WO 2017051437 A3 WO2017051437 A3 WO 2017051437A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant
nucleotide sequence
dehydrocholesterol reductase
organisms
sequence encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IN2016/050323
Other languages
French (fr)
Other versions
WO2017051437A2 (en
Inventor
Krishna MURALIDHARAN
Anupama Datla
Trupti Krishnakant Ashar
Williams Rajasekar Vyasarayani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fermenta Biotech Ltd
Original Assignee
Fermenta Biotech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fermenta Biotech Ltd filed Critical Fermenta Biotech Ltd
Publication of WO2017051437A2 publication Critical patent/WO2017051437A2/en
Publication of WO2017051437A3 publication Critical patent/WO2017051437A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invneiton provides a novel nucleotide sequence represented by Seq ID No.1 encoding 7-dehydrocholesterol reductase (7-DHCR), recombinant DNA constructs and recombinant micro-organisms carrying the said nucleotide sequence. Further, the present invention relates to a process for the production of 7-DHC in the presence of a recombinant 7-DHCR and its application in the synthesis of vitamin D3.
PCT/IN2016/050323 2015-09-24 2016-09-24 A nucleotide sequence encoding 7-dehydrocholesterol reductase, recombinant constructs and recombinant organisms comprising said sequence Ceased WO2017051437A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN3636/MUM/2015 2015-09-24
IN3636MU2015 2015-09-24

Publications (2)

Publication Number Publication Date
WO2017051437A2 WO2017051437A2 (en) 2017-03-30
WO2017051437A3 true WO2017051437A3 (en) 2017-05-11

Family

ID=57256387

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2016/050323 Ceased WO2017051437A2 (en) 2015-09-24 2016-09-24 A nucleotide sequence encoding 7-dehydrocholesterol reductase, recombinant constructs and recombinant organisms comprising said sequence

Country Status (1)

Country Link
WO (1) WO2017051437A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112154213B (en) * 2018-05-22 2025-02-28 帝斯曼知识产权资产管理有限公司 Optimization of C-5 sterol desaturation
CN113082208B (en) * 2019-12-23 2022-07-08 中国科学院分子细胞科学卓越创新中心 Medicine for blocking microbial infection, reducing cholesterol and preventing and treating related tumors and application thereof
CN114854812B (en) * 2022-03-11 2024-08-27 陕西岳达德馨生物制药有限公司 Method for improving efficiency of enzyme catalysis production of ursodeoxycholic acid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060240508A1 (en) * 2002-01-29 2006-10-26 Basf Aktiengesellschaft Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060240508A1 (en) * 2002-01-29 2006-10-26 Basf Aktiengesellschaft Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WATERHAM H R ET AL: "SMITH-LEMLI-OPITZ SYNDROME IS CAUSED BY MUTATIONS IN THE 7-DEHYDROCHOLESTEROL REDUCTASE GENE", 1 January 1998, AMERICAN JOURNAL OF HUMAN GENETICS, AMERICAN SOCIETY OF HUMAN GENETICS, CHICAGO, IL, US, PAGE(S) 329 - 338, ISSN: 0002-9297, XP001166903 *

Also Published As

Publication number Publication date
WO2017051437A2 (en) 2017-03-30

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