WO2016121923A1 - Muscle synthesis promoter - Google Patents

Abstract

The present invention pertains to a muscle synthesis promoter which exhibits a muscle synthesis promotion action, and with which it is possible to efficiently ingest milk protein. More specifically, the present invention pertains to a muscle synthesis promoter containing, as active ingredients, 0.2-1.2 wt.% of a soybean polysaccharide, 0.1-0.5 wt.% of a HM pectin, 0.01-0.1 wt.% of a fibrous insoluble cellulose, and an acidic liquid composition that contains a milk protein mixture including a casein protein and a whey protein. This muscle synthesis promoter is capable of improving the muscle synthesis promotion effect by preventing the curdling of the milk protein and promoting the absorption of the milk protein in the stomach.

Description

Muscle synthesis promoter Reference to related applications

This patent application is accompanied by a priority claim based on Japanese Patent Application No. 2015-016093 (filing date: January 29, 2015) which is a previously filed Japanese patent application. The entire disclosure in is hereby incorporated by reference into the present disclosure.

The present invention relates to a muscle synthesis promoter.

In recent years, for the purpose of promoting health and improving exercise capacity, in order to promote muscle synthesis, prevent muscle loss, and promote recovery of muscle mass, orally ingested a protein-based nutritional supplement (supplement), There are many people (subjects) who exercise. As such a nutritional supplement, there is protein powder, and as its main component, whey protein (whey protein) and casein protein made from milk are known.

Whey protein has a high rate (absorption time) from ingestion to digestion and absorption (short time) and contains a lot of BCAA (branched chain amino acids: valine, leucine, isoleucine), thus preventing muscle fatigue, It is said that muscles can be strengthened efficiently. On the other hand, casein protein is metabolized slowly and continuously compared to whey protein, so the rate from absorption to digestion and absorption (slow absorption rate) is slow (long time), and immediate muscle synthesis promotion is required. It is said that it is unsuitable for the target (see Japanese Patent Application Publication No. 2009-507044). Therefore, whey protein is mainly used as protein powder.

On the other hand, a muscle synthesis promoting effect is also known for milk protein containing casein protein and whey protein in predetermined amounts. Specifically, it is known that intake of milk protein after exercise promotes skeletal muscle synthesis in a dose-dependent manner and suppresses skeletal muscle degradation (Non-patent Document 1: Proceedings of the 64th Annual Meeting of the Japanese Society of Physical Education) Shu, 2013, page 166). Furthermore, according to the Non-patent Document 1: Proceedings of the 64th Annual Meeting of the Japanese Society of Physical Education, 2013, page 166, milk protein-enriched milk drinks are associated with exercise load compared to normal milk as food to be taken immediately after exercise It has been shown to be more effective in making the body.

That is, in order to ingest milk protein and promote muscle synthesis, the intake of total milk protein, such as ingesting high-concentration milk protein-enriched milk drinks or large amounts of normal milk Is effective.

Special table 2009-507044 gazette

Proceedings of the 64th Annual Meeting of the Japanese Society of Physical Education, 2013, 166 pages

In order to increase the overall intake of milk protein, such as taking a high-concentration milk protein-enriched milk drink or taking a large amount of normal milk, it is said that a large amount of milk protein must be consumed A problem occurs. Ingestion of such a large amount of milk protein sometimes makes it difficult for infants and the elderly who cannot take a large amount of milk protein as energy, and further, such as obesity accompanying excessive intake of energy There are also concerns about the development of lifestyle-related diseases. In addition, taking a large amount of milk protein may be a burden regardless of age. In particular, immediately after exercise, although it is necessary to promptly promote muscle synthesis, the appetite is reduced due to hormone secretion by exercise, so that it is considered that a large amount of milk protein intake becomes a burden.

As described above, a neutral liquid composition containing milk protein, such as Non-patent Document 1: Proceedings of the 64th Annual Meeting of the Japanese Society of Physical Education, 2013, page 166, for the purpose of intake of a large amount of milk protein is used as an active ingredient. A muscle synthesis promoter that does not necessarily require the intake of a large amount of milk protein and has a muscle synthesis promotion effect equivalent to or higher than the muscle synthesis promotion effect is known. Therefore, it has been a problem to provide a new muscle synthesis promoter that exhibits such effects.

As a result of intensive studies to solve the above problems, the present inventors have found that 0.2 to 1.2% by weight of soybean polysaccharide, 0.1 to 0.5% by weight of HM pectin, 0.01 to An acidic liquid composition containing 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein at a specific protein ratio is easy to ingest and consumes a large amount of milk protein. Even without this, I found a phenomenon that it has an effect of promoting muscle synthesis.

Specifically, the present inventors now know that milk protein contributes to the muscle synthesis promoting effect in a dose-dependent manner in the prior art, but 0.2 to 1.2% by weight. Milk containing 0.1 to 0.5 wt.% HM pectin, 0.01 to 0.1 wt.% Fibrous insoluble cellulose, and a specific protein amount ratio of casein protein and whey protein The acidic liquid composition containing a protein mixture is a non-fat dry milk solution, that is, a muscle synthesis promoter containing a neutral liquid composition consisting only of a large amount of milk protein mixture as an active ingredient (for example, Non-patent Document 1: The Japanese Society of Physical Education) Compared to the 64th Annual Meeting Proceedings, 2013, p. 166), we have unexpectedly found a phenomenon that there is an immediate effect of promoting muscle synthesis. 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous insoluble cellulose, and a specific amount of protein An acidic liquid composition containing a milk protein mixture containing a ratio of casein protein and whey protein is effective as a neutral liquid composition consisting only of a large amount of milk protein mixture, even if the acidic liquid composition has a low milk protein concentration. We have unexpectedly found a phenomenon that it has the same muscle synthesis promoting action as compared with the muscle synthesis promoter used as a component. The above phenomenon was surprising. The present invention is based on such knowledge.

That is, the present invention is as follows.
[1] 0.2-1.2% by weight of soybean polysaccharide, 0.1-0.5% by weight of HM pectin, 0.01-0.1% by weight of fibrous insoluble cellulose, and casein protein A muscle synthesis promoter comprising an acidic liquid composition containing a milk protein mixture containing whey protein as an active ingredient, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20 A muscle synthesis promoter,
[2] The muscle synthesis promoter according to [1], wherein the acidic liquid composition further comprises 0.5 to 5% by weight of trehalose,
[3] The muscle synthesis promoter according to [1] or [2], wherein the acidic liquid composition has a pH of 3 to 6,
[4] The muscle synthesis promoter according to any one of [1] to [3], wherein the fibrous insoluble cellulose is fermented cellulose,
[5] In any one of [1] to [4], the milk protein mixture contained in the acidic liquid composition is ingested so that the total protein amount is 0.06 to 0.7 g / kg body weight per serving. The muscle synthesis promoter according to the description,
[6] The unit packaging form according to any one of [1] to [5], comprising a unit packaging form per serving, wherein the unit packaging form contains a milk protein mixture in a total protein amount of 3 to 40 g as a meal intake. Of muscle synthesis promoter,
[7] The muscle synthesis promoter according to any one of [1] to [6] for a subject to exercise,
[8] The muscle synthesis promoter according to [7], wherein the exercise is muscle training,
[9] 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous insoluble cellulose, and casein protein A method for promoting muscle synthesis, comprising ingesting an acidic liquid composition containing a milk protein mixture containing whey protein to a subject in need of muscle synthesis promotion, wherein the protein amount ratio of casein protein to whey protein (Casein protein / whey protein) is 2 to 20, a method for promoting muscle synthesis,
[10] 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous insoluble cellulose, and casein protein A method for promoting muscle synthesis (excluding medical practices for humans), comprising ingesting an acidic liquid composition comprising a milk protein mixture containing whey protein to a subject in need of muscle synthesis promotion, wherein whey A method for promoting muscle synthesis, wherein the protein amount ratio of casein protein to protein (casein protein / whey protein) is 2 to 20,
[11] 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous to promote muscle synthesis Use of an insoluble cellulose, and an acidic liquid composition comprising a milk protein mixture containing casein protein and whey protein as an active ingredient, wherein the ratio of casein protein to whey protein (casein) Protein / whey protein) is 2-20, use,
[12] 0.2 to 1.2% by weight of soybean polysaccharide, 0.1 to 0.5% by weight of HM pectin, 0.01 to 0.5% for the production of foods or pharmaceuticals for promoting muscle synthesis Use of a muscle synthesis promoter comprising as an active ingredient an acidic liquid composition comprising 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein, Use, wherein the protein amount ratio of casein protein (casein protein / whey protein) is 2-20
[13] 0.2-1.2 wt% soy polysaccharide, 0.1-0.5 wt% HM pectin, 0.01-0.1 wt% fibrous insoluble cellulose, and casein protein An acidic liquid composition comprising a milk protein mixture containing whey protein, wherein the protein content ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20, acidic to promote muscle synthesis Liquid composition.

ADVANTAGE OF THE INVENTION According to this invention, after making the milk protein curd in the stomach and promoting absorption of milk protein, the effect of promoting muscle synthesis can be enhanced, and a neutral liquid containing milk protein at the same concentration The muscle synthesis promoting effect equivalent to or higher than the muscle synthesis promoting effect comprising the composition as an active ingredient can be exhibited. Therefore, according to the present invention, an effect of promoting muscle synthesis can be achieved without necessarily ingesting a large amount of milk protein.

The rate of skeletal muscle synthesis (FSR (% / day)) when the muscle synthesis promoter of the present invention (milk protein 3.4 wt%) and skim milk powder solution (milk protein 3.4 wt%) were orally administered. It shows the transition. Skeletal muscle synthesis rate (FSR (% / day) when orally administered with the muscle synthesis promoter of the present invention (milk protein 3.4 wt%) and a high-dose skim milk solution (milk protein 6.8 wt%). ).

Detailed description of the invention

Hereinafter, the present invention will be described in detail, but the present invention is not limited to individual forms.

The muscle synthesis promoter of the present invention comprises an acidic liquid composition as an active ingredient, and is preferably a muscle synthesis promoter containing an acidic liquid composition as an active ingredient. Specifically, the muscle synthesis promoter of the present invention is an acidic liquid composition comprising a soy polysaccharide, HM pectin, fibrous insoluble cellulose, and a milk protein mixture containing casein protein and whey protein in a specific protein ratio. It is a muscle synthesis promoter comprising a product as an active ingredient. More specifically, the muscle synthesis promoter of the present invention comprises 0.2 to 1.2% by weight soybean polysaccharide, 0.1 to 0.5% by weight HM pectin, 0.01 to 0.1% by weight. % Muscle insoluble cellulose, and a muscle synthesis promoter comprising an acidic liquid composition containing a milk protein mixture containing casein protein and whey protein as an active ingredient, the protein amount ratio of casein protein to whey protein It is a muscle synthesis promoter having (casein protein / whey protein) of 2 to 20. According to a preferred embodiment of the present invention, the muscle synthesis promoter of the present invention is used for a subject in need of immediate acceleration of muscle synthesis.

“Active ingredient” means an ingredient required for exerting a muscle synthesis promoting effect. As one preferred form, the “active ingredient” means an ingredient required for exerting a muscle synthesis promoting effect for a subject requiring immediate muscle synthesis promotion.

“Promoting muscle synthesis” means promoting the synthesis of muscle by taking milk protein and absorbing it in the body as a nutrient. Therefore, the muscle synthesis promoter of the present invention is used for that and the state resulting therefrom. For example, the purpose of muscle synthesis promotion includes muscle gain, muscle growth promotion, muscle tissue repair, muscle strengthening, muscle strength improvement, health promotion effect and muscle performance improvement accompanying muscle synthesis promotion, preferably muscle tissue repair, It is the improvement of muscular strength, the health promotion effect accompanying the promotion of muscle synthesis and the improvement of exercise ability. Promoting muscle synthesis also includes assimilation of muscle proteins from amino acids. “Immediately effective muscle synthesis promotion” means promoting muscle synthesis accompanying immediate absorption of milk protein.

“Subjects needing muscle synthesis promotion” means subjects who need to promote muscle protein synthesis in order to repair muscle tissue and promote muscle growth. For the purpose of this, it means a subject who exercises to promote muscle synthesis, prevent muscle loss, and promote recovery of muscle mass. Here, the exercise is preferably strength training (resistance exercise), and more preferably long-term strength training, for example, in that muscle synthesis is promoted by applying a certain load to muscle strength. It is strength training that is continued for 30 minutes to 5 hours at a frequency of 1 to 7 days in a week. Examples of strength training include dumbbell exercise, squat exercise, and machine training.

For the purpose of promoting muscle synthesis of the present invention, for example, athletes, sports enthusiasts (athletes), those who need exercise to improve lifestyle-related diseases, elderly people, etc. Examples include those who require exercise, or patients who suffer from a disease requiring muscle synthesis promotion, such as a muscle injury disease (eg, sarcopenia), and need to promote muscle synthesis through exercise. In addition, the target may be an animal other than a human (domestic animals such as horses and cows, pets such as dogs and cats, appreciation animals kept in zoos, and the like).

The time when the muscle synthesis promoter of the present invention is ingested (administered) to a subject in need of muscle synthesis may be before exercise, after exercise, or during exercise. Here, the time of intake is preferably after exercise, more preferably immediately after exercise. In terms of suppressing skeletal muscle degradation (catabolism) caused by exercise and promoting assimilation from amino acids to proteins, the intake period is preferably within 3 hours after the end of exercise, immediately after exercise. More preferably, within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, and particularly preferably within 30 minutes after the end of exercise (Levenhagenhaet al. Am J physiol Endocrinol Metab (2001), 280, E982-E993).

In the muscle synthesis promoting effect, the neutral muscle composition promoting effect equivalent to a neutral liquid composition containing milk protein at the same concentration is a neutral liquid composition containing milk protein at the same concentration (for example, milk, skim milk, It means the same level of the rate of protein absorption and the same effect as the muscle synthesis promoting effect associated with the absorption observed when orally ingested (administered) (concentrated milk, skim concentrated milk, etc.). Further, in the effect of promoting muscle synthesis, an immediate effect of promoting muscle synthesis equivalent to a neutral liquid composition containing milk protein at the same concentration is a neutral liquid composition containing milk protein at the same concentration (for example, , Milk, skim milk, concentrated milk, concentrated skim milk, etc.), the same level of protein absorption rate and the same level of muscle synthesis promoting effect associated with the absorption Means effect. Here, the effect of promoting muscle synthesis can be confirmed by, for example, measuring the upper limb triceps muscle synthesis rate (FSR) within 1 hour after administration of the test substance after the swimming test of rats. it can. That is, the FSR of the test group is comparable or comparable to the FSR of the control group that orally ingested a neutral liquid composition containing milk protein that is said to have an immediate effect of promoting muscle synthesis at the same concentration. In the above case, it can be determined that the muscle synthesis promoting action is significant. In this case, the immediate effect of promoting muscle synthesis is preferably a rate of synthesizing muscle in a short time after ingesting the muscle synthesis promoter of the present invention, more preferably within 30 minutes to 3 hours after ingestion, more preferably Can be confirmed by measuring the rate of muscle synthesis within 30 minutes to 2 hours after ingestion, more preferably within 30 minutes to 1 hour after ingestion, and particularly preferably 30 minutes after ingestion.

"Protein amount" means the amount of protein as a polymer of amino acids contained in various proteins such as milk protein, whey protein or casein protein. “Protein amount ratio” means the ratio of the amount of protein as a polymer of amino acids contained in various proteins such as milk protein, whey protein, or casein protein. Here, the amount of protein may be calculated based on known information such as a food composition table, or may be calculated by measuring by a conventional method such as Kjeldahl method or Raleigh method. Actually, in the case of the Kjeldahl method, it can be calculated by measuring nitrogen contained in various proteins and multiplying the value by a nitrogen-protein conversion factor (usually 6.25).

The acidic liquid composition used in the present invention includes 0.2 to 1.2% by weight of soybean polysaccharide, 0.1 to 0.5% by weight of HM pectin, and 0.01 to 0.1% by weight of fiber. And a milk protein mixture containing casein protein and whey protein in a specific protein amount ratio, and an acidified liquid composition. The acidic liquid composition used in the present invention may not be completely liquid, and may be partly solid and / or gel.

The milk protein mixture contained in the acidic liquid composition used in the present invention is not particularly limited as long as it contains whey protein and casein protein in a specific protein amount ratio, and is separated and purified separately from whey protein. Casein protein may be prepared by mixing to a specific protein amount ratio, or it may already contain whey protein and casein protein that have not been separated or purified separately at a specific protein amount ratio. You may mix and prepare a raw material (raw material), a foodstuff, etc. At this time, the milk protein mixture used in the present invention does not require a separation step or purification step of whey protein or casein protein, and the production cost thereof is low, so that whey protein and casein protein are already specified. It can be prepared by mixing the raw materials and foods contained in the protein amount ratio as the main components, and mixing the whey protein and casein protein, which are separately purified, if necessary, so that the specific protein amount ratio is obtained. preferable.

Examples of whey protein separated and purified separately from casein protein include whey stock solutions (sweet whey, acid whey, etc.), concentrates, dried products (whey powder, etc.), frozen products, hydrolyzed products, etc. And reducing solutions thereof. Further, desalted whey, whey protein concentrate (WPC), purified whey protein (WPI), α-lactalbumin (α-La), β-lactoglobulin (β-Lg), immunoglobulin, lactoferrin, and the like, and These reducing solutions can be used. Casein protein separated and purified separately from whey protein includes, for example, casein isolate (natural cheese such as fresh cheese, sodium caseinate, calcium caseinate), its concentrate, its dried product, its A frozen material, a hydrolyzate thereof, a reducing solution thereof, and the like can be used. Furthermore, purified products such as α-casein, β-casein, k-casein, and reducing solutions thereof can be used. In addition, raw materials and foods that already contain whey protein and casein protein in a specific protein amount ratio include, for example, raw milk, pasteurized milk (such as milk), skim milk, component-adjusted milk, processed milk, and dairy products (concentrated) Milk, powdered milk, condensed milk, fermented milk (such as yogurt), lactic acid bacteria beverages, processed cheeses, ice creams, creams), milk protein concentrate (MPC), its concentrate, its dried product, its frozen product, its water Decomposed products and the like, and reducing solutions thereof can be used. Among these, the milk protein mixture is a milk protein concentrate that is a milk raw material rich in casein protein in that it is easy to prepare an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein. (MPC) is preferably used. In this milk protein concentrate, the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 4. At this time, you may obtain and use a commercial item for these, and may prepare and use it for themselves.

The milk protein mixture contained in the acidic liquid composition used in the present invention is composed of whey protein and casein protein in a specific protein amount ratio. Specifically, the protein amount ratio between casein protein and whey protein ( Casein protein / whey protein) is 2 to 20, preferably 2 to 10, more preferably 2 to 6, and further preferably 2 to 4. In the present invention, the protein amount ratio between casein protein and whey protein means the protein amount ratio in the milk protein mixture contained in the acidic liquid composition contained in the muscle synthesis promoter. In the milk protein mixture used in the present invention, the amount ratio of casein protein to whey protein (casein protein / whey protein) is intentionally adjusted (designed), and further prepared into an acidic liquid composition. In this respect, it is very different from the prior art.

The acidic liquid composition used in the present invention generally has a milk protein mixture containing a high concentration of casein protein, where the casein protein in the milk protein is likely to coagulate with acid and separation and precipitation resulting from the acid coagulation occur. Even the contained acidic liquid composition is in a form that suppresses separation and precipitation, has good redispersibility, is low in viscosity, and is easy to ingest (administer). If it is the range applicable to this form, the detailed property (pH, viscosity, etc.) can be designed arbitrarily.

The pH of the acidic liquid composition used in the present invention is, for example, 3 to 6, 3.1 to 5.8, 3.2 to 5.6, 3.3 to 5.4, 3.4 to 5.2. 3.5-5, 3.6-4.8, 3.7-4.6, 3.8-4.4. It is preferable that the pH of the acidic liquid composition of the present invention exceeds 3 in order to prevent the acidity from being felt strongly and difficult to take. Moreover, it is preferable that the pH of the acidic liquid composition of the present invention is less than 6 in order to prevent the acidity from being felt weakly refreshing and difficult to take as an acidic liquid composition.

The viscosity (10 ° C.) of the acidic liquid composition used in the present invention is the viscosity of the acidic liquid composition measured with a B-type rotational viscometer. The viscosity (10 ° C.) of the acidic liquid composition used in the present invention is, for example, 3 to 30 mPa · s, 3.5 to 30 mPa · s, 4 to 30 mPa · s, 4.5 to 30 mPa · s, 5 to 30 mPa. · S, 5.5 to 29 mPa · s, 6 to 28 mPa · s, 6.5 to 27 mPa · s, 7 to 26 mPa · s, 7.5 to 25 mPa · s, 8 to 24 mPa · s, 8.5 to 23 mPa S, 9 to 22 mPa · s, 9.5 to 21 mPa · s, and 10 to 20 mPa · s. When the viscosity (10 ° C.) of the acidic liquid composition used in the present invention exceeds 3 mPa · s, the casein protein of the acidic liquid composition becomes difficult to stabilize, and the acidic liquid composition is likely to be separated and precipitated. It is preferable for preventing the above. Moreover, it is preferable that the viscosity (10 ° C.) of the acidic liquid composition used in the present invention is less than 30 mPa · s in order to prevent refreshing from being impaired and difficult to take. The viscosity (10 ° C.) of the acidic liquid composition used in the present invention is the viscosity of the acidic liquid composition measured with a general-purpose B-type rotational viscometer such as a Brookfield viscometer.

The acidic liquid composition used in the present invention comprises a milk protein mixture containing casein protein and whey protein in a specific protein amount ratio. Here, the casein protein contained in the milk protein mixture is generally stable in a neutral region, but in an environment in a pH region lower than around the isoelectric point of pH 4.6, casein protein is caused by acid coagulation. Aggregates with each other, leading to separation of the acidic liquid composition and occurrence of precipitation. On the other hand, even if the acidic liquid composition comprising a specific composition used in the present invention is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, separation and precipitation of the acidic liquid composition is suppressed. In addition, the redispersibility is good, and the form is low in viscosity and easy to ingest. When the concentration of the milk protein mixture containing casein protein contained in the acidic liquid composition used in the present invention is high, for example, 3% by weight or more and 3.2% by weight 3.4% or more, 3.6% or more, 3.8% or more, 4% or more, 4.2% or more, 4.4% or more, 3 to 10%, 3 to 9.5 wt%, 3-9 wt%, 3-8.5 wt%, 3-8 wt%, 3-7.5 wt%, 3-7 wt%, 3.2-10 wt%, 2 to 9.5 wt%, 3.2 to 9 wt%, 3.2 to 8.5 wt%, 3.2 to 8 wt%, 3.2 to 7.5 wt%, 3.2 to 7 wt% %, 3.4-10 wt%, 3.4-9.5 wt%, 3.4-9 wt%, 3.4-8.5 wt%, 3.4-8 wt%, 3.4- 7.5% by weight, 3.4-7 layers %, 3.6 to 10% by weight, 3.6 to 9.5% by weight, 3.6 to 9% by weight, 3.6 to 8.5% by weight, 3.6 to 8% by weight, 3.6 to 7.5 wt%, 3.6-7 wt%, 3.8-10 wt%, 3.8-9.5 wt%, 3.8-9 wt%, 3.8-8.5 wt%, 3.8-8 wt%, 3.8-7.5 wt%, 3.8-7 wt%, 4-10 wt%, 4-9.5 wt%, 4-9 wt%, 4-8. 5 wt%, 4-8 wt%, 4-7.5 wt%, 4-7 wt%, 4.2-10 wt%, 4.2-9.5 wt%, 4.2-9 wt%, 4.2-8.5 wt%, 4.2-8 wt%, 4.2-7.5 wt%, 4.2-7 wt%, 4.4-10 wt%, 4.4-9. 5 wt%, 4.4-9 wt%, 4.4-8.5 wt%, 4.4-8 wt%, 4.4-7.5 wt%, 4.4 7 percent by weight. Further, when the concentration of the milk protein mixture containing casein protein contained in the acidic liquid composition used in the present invention is not high, it is, for example, 3% by weight or less, 2.8% by weight or less, 2 .6% by weight or less, 2.4% by weight or less, 2.2% by weight or less, 2% by weight or less, 0.5 to 3% by weight, 1 to 3% by weight, 1.2 to 3% by weight, 1.4 ~ 3 wt%, 1.6-3 wt%, 1.8-3 wt%, 0.5-2.8 wt%, 1-2.8 wt%, 1.2-2.8 wt%, 1 4.4 to 2.8 wt%, 1.6 to 2.8 wt%, 1.8 to 2.8 wt%, 0.5 to 2.6 wt%, 1 to 2.6 wt%, 1.2 ~ 2.6 wt%, 1.4-2.6 wt%, 1.6-2.6 wt%, 1.8-2.6 wt%, 0.5-2.4 wt%, 1-2 .4% by weight, 1.2-2.4% by weight, 1.4 2.4 wt%, 1.6-2.4 wt%, 1.8-2.4 wt%, 0.5-2.2 wt%, 1-2.2 wt%, 1.2-2. 2% by weight, 1.4-2.2% by weight, 1.6-2.2% by weight, 1.8-2.2% by weight, 0.5-2% by weight, 1-2% by weight, 2 to 2% by weight, 1.4 to 2% by weight, 1.6 to 2% by weight, and 1.8 to 2% by weight. Here, each concentration of the casein protein and the whey protein contained in the milk protein mixture can be easily calculated from the protein amount ratio of the casein protein and the whey protein described above.

Even if the acidic liquid composition used in the present invention is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, separation and precipitation of the acidic liquid composition are suppressed, and redispersibility is also good. In order to increase the muscle synthesis promoting effect while providing the characteristics of a low-viscosity and easily ingested form, preventing the curding of milk protein in the stomach, and promoting the absorption of milk protein. It may contain 2 to 1.2% by weight soybean polysaccharide, 0.1 to 0.5% by weight HM pectin, 0.01 to 0.1% by weight fibrous insoluble cellulose.

The soybean polysaccharide of the present invention may be one containing one or more of rhamnose, galactose, arabinose, xylose, glucose and uronic acid derived from soybean, and among soybeans, those derived from cotyledons are preferable. The molecular weight of the soybean polysaccharide of the present invention is not particularly limited and is preferably a high molecular weight, and preferably has an average molecular weight of several thousand to several million, specifically 5,000 to 1,000,000. . The average molecular weight of the soybean polysaccharide is a value determined by an intrinsic viscosity method using a standard pullulan (Showa Denko KK) as a standard substance and measuring the viscosity in a 0.1M NaNO ₃ solution. Such soybean polysaccharides are commercially available, for example, SM-700 (manufactured by San-Ei Gen FFI Co., Ltd.) and SM-900 (manufactured by San-Ei Gen FFI Co., Ltd.). Soy polysaccharides are said to suppress the separation and precipitation of acidic liquid compositions caused by acid coagulation between casein proteins in acidic liquid compositions due to the structure of their side chains.

The addition amount of the soybean polysaccharide of the present invention is 0.1 to 0.5% by weight of HM pectin and 0.01 to 0.1% by weight of fibrous material with respect to the acidic liquid composition used in the present invention. When insoluble cellulose is contained, for example, 0.2 to 1.2% by weight, 0.25 to 1.15% by weight, 0.3 to 1.1% by weight, 0.35 to 1.05% by weight 0.4 to 1 wt%, 0.4 to 0.9 wt%. When the added amount of the soybean polysaccharide of the present invention is less than 1.2% by weight, the acidic liquid composition can feel a unique flavor of soybean, and it is difficult to prevent the acidic liquid composition from being ingested. Is preferable. Further, the addition amount of the soybean polysaccharide of the present invention exceeds 0.2% by weight, even if it is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein. Separation of liquid composition and occurrence of precipitation can be suppressed, and redispersibility is also improved. Finally, when taken as a muscle synthesis promoter of the present invention, milk protein curdization in the stomach is achieved. It is preferable in terms of preventing, promoting absorption of milk protein, and enhancing the effect of promoting muscle synthesis.

The HM pectin of the present invention is an acidic polysaccharide having α-D-galacturonic acid as a main chain component, which exists as a cell wall component in vegetables and fruits. Galacturonic acid constituting pectin is partially methyl esterified and is divided into LM pectin and HM pectin depending on the degree of esterification. HM pectin generally has a degree of esterification of 50% or more. Such HM pectin is commercially available, for example, SM-478 (manufactured by Saneigen FFI, Inc.), SM-666 (manufactured by Saneigen FFI, Inc.), AYD5110SB (manufactured by Unitech Foods, Inc.). )and so on. HM pectin is characterized by being negatively charged, and is said to prevent aggregation of casein proteins by binding to the casein protein, and the bound casein proteins repel each other due to the charge.

The added amount of HM pectin of the present invention is 0.2 to 1.2% by weight of soybean polysaccharide and 0.01 to 0.1% by weight of fibrous form with respect to the acidic liquid composition used in the present invention. When insoluble cellulose is contained, for example, 0.1 to 0.5% by weight, 0.1 to 0.45% by weight, 0.1 to 0.4% by weight, 0.1 to 0.35% by weight 0.1-0.3 wt%, 0.12-0.29 wt%, 0.14-0.28 wt%, 0.16-0.27 wt%, 0.18-0.26 wt% 0.2 to 0.25% by weight. When the added amount of HM pectin of the present invention is less than 0.5% by weight, even an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein has a low viscosity and is easily ingested. Preferred above. Moreover, even if it is an acidic liquid composition containing the milk protein mixture containing a high concentration casein protein that the addition amount of HM pectin of this invention exceeds 0.1 weight%, the acidic liquid used by this invention Separation of the composition and occurrence of precipitation can be suppressed, and redispersibility is also improved. Finally, when taken as a muscle synthesis promoter of the present invention, prevention of curding milk protein in the stomach is prevented. It is preferable in terms of promoting absorption of milk protein and enhancing muscle synthesis promoting effect.

Even if the acidic liquid composition used in the present invention is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, separation and precipitation of the acidic liquid composition is suppressed, and redispersibility is also achieved. It is a good, low-viscosity, easy-to-ingest form, and when ingested as a muscle synthesis promoter of the present invention, it prevents the formation of curd of milk protein in the stomach and promotes absorption of milk protein and then muscles. In order to enhance the synthesis promoting effect, other stabilizers such as sodium carboxymethylcellulose and / or alginate propylene glycol ester may be used in combination with soybean polysaccharide and / or HM pectin or in place of soybean polysaccharide and / or HM pectin. Can be added. In particular, other stabilizers, such as sodium carboxymethylcellulose and / or propylene glycol alginate, have a lower viscosity and inhibit the separation and precipitation of acidic liquid compositions at lower viscosity compared to soy polysaccharides and / or HM pectin. In order to enhance the muscle synthesis promoting effect while preventing the curding of milk protein in the stomach and promoting the absorption of milk protein when taken as a muscle synthesis promoter, the dispersibility is also good. It is preferably used in combination with polysaccharides and / or HM pectin, or instead of soybean polysaccharides and / or HM pectin.

In the acidic liquid composition used in the present invention, sodium carboxymethyl cellulose can be added. Sodium carboxymethyl cellulose is obtained by substituting a carboxymethyl group for the hydroxyl group of glucose of cellulose, and usually has a degree of etherification of about 0.6 to 1.5. Such sodium carboxymethylcellulose is commercially available, for example, Serogen F-SL (Daiichi Kogyo Seiyaku), Serogen F-810A (Daiichi Kogyo Seiyaku), Serogen F-SB (Daiichi Kogyo). Pharmaceutical Co., Ltd.), Serogen F-820B (Daiichi Kogyo Seiyaku Co., Ltd.), Serogen F-1220B (Daiichi Kogyo Seiyaku Co., Ltd.), and the like.

Alginic acid propylene glycol ester can be added to the acidic liquid composition used in the present invention. Alginate propylene glycol ester is a substance obtained by chemically modifying polysaccharide alginic acid purified from algal algae such as kombu and wakame. Such propylene glycol alginate is commercially available. For example, Tac Lloyd LF-M (manufactured by Kikkoman Biochemifa), Tacroid EF (manufactured by Kikkoman Biochemifa), Tacroid PF (manufactured by Kikkoman Biochemifa) ), Tuck Lloyd PF-H (manufactured by Kikkoman Biochemifa).

The addition amount of sodium carboxymethylcellulose or propylene glycol alginate of the present invention is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, which is a feature of the acidic liquid composition used in the present invention. In addition, it suppresses separation and precipitation of acidic liquid composition, has good redispersibility, is in a form that is low in viscosity and easy to ingest, and when ingested as a muscle synthesis promoter, it is a milk protein card in the stomach. As long as the effect of promoting muscle synthesis can be enhanced while preventing absorption and promoting absorption of milk protein, the amount added is not limited. The amount of sodium carboxymethylcellulose or propylene glycol alginate added to the acidic liquid composition used in the present invention is, for example, 0.01 to 2% by weight, 0.01 to 1.5% by weight, 0.01 to 1.2% by weight, 0.01 to 1.0% by weight. If the addition amount of sodium carboxymethylcellulose or propylene glycol alginate of the present invention is 2% by weight, even an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein can be easily ingested with low viscosity. It is preferable in terms of form. Moreover, even if it is an acidic liquid composition containing the milk protein mixture containing a high concentration casein protein that the addition amount of the sodium carboxymethylcellulose or propylene glycol alginate of the present invention exceeds 0.01% by weight, Separation of acidic liquid composition used in the invention and generation of precipitation can be suppressed, and redispersibility is also improved. Finally, when taken as a muscle synthesis promoter, a card of milk protein in the stomach This is preferable in that it prevents the formation of protein, promotes the absorption of milk protein, and enhances the muscle synthesis promoting effect of the present invention.

The fibrous insoluble cellulose of the present invention is contained in the acidic liquid composition used in the present invention, and by constructing a three-dimensional network structure in the acidic liquid composition, dispersion of insoluble components such as precipitates is achieved. It is a stabilizer that can improve the properties. The fibrous insoluble cellulose of the present invention may be an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, which is a characteristic of the acidic liquid composition used in the present invention. Inhibits separation and precipitation, has good redispersibility, has a low viscosity and is easily ingested, and when ingested as a muscle synthesis promoter, prevents milk protein curding in the stomach and absorbs milk protein. If the effect of promoting muscle synthesis can be enhanced after being promoted, the origin thereof is not limited, and those derived from plants and / or microorganisms can be used. The fibrous insoluble cellulose derived from a microorganism is, for example, one obtained by separating a fermented cellulose produced by a microorganism from a culture medium, washing it, and appropriately purifying it into a fibrous form. The fibrous insoluble cellulose is, for example, fibrous insoluble cellulose (see JP 2005-245217 A) that has been microfibrillated to an average diameter of 0.01 to 0.1 μm, or fermented cellulose. The fibrous insoluble cellulose of the present invention is commercially available, for example, Sun Artist PG (manufactured by Saneigen FFI), Sun Artist PN (manufactured by Saneigen FFI). .

The addition amount of the fibrous insoluble cellulose of the present invention is such that 0.2 to 1.2% by weight of soybean polysaccharide and 0.1 to 0.5% by weight of HM pectin are added to the acidic liquid composition used in the present invention. In the case of inclusion, for example, 0.01 to 0.1% by weight, 0.015 to 0.09% by weight, 0.02 to 0.08% by weight, 0.025 to 0.085% by weight, 0.0. They are 03-0.07 wt%, 0.03-0.065 wt%, 0.03-0.06 wt%, 0.03-0.055 wt%, 0.03-0.05 wt%. If the addition amount of the fibrous insoluble cellulose of the present invention is 0.1% by weight, even an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein has a low viscosity and is easily ingested. Is preferable. Further, the addition amount of the fibrous insoluble cellulose of the present invention exceeds 0.01% by weight, even in the case of an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein. Separation of the composition and occurrence of precipitation can be suppressed, and redispersibility is also improved. Finally, when taken as a muscle synthesis promoter, curding of milk protein in the stomach is prevented, and milk protein It is preferable in that the absorption of glycine is promoted and the muscle synthesis promoting effect of the present invention is enhanced.

Trehalose can be further added to the acidic liquid composition used in the present invention, and by adding trehalose, even an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, Suppresses separation and precipitation of the acidic liquid composition, has good redispersibility, and can be further imparted with a low-viscosity and easy-to-ingest form. Finally, it is ingested as a muscle synthesis promoter of the present invention. In this case, curding of milk protein in the stomach can be prevented, absorption of milk protein can be promoted, and the effect of promoting muscle synthesis can be further enhanced. Specifically, by adding trehalose to the acidic liquid composition used in the present invention, the pH of the acidic liquid composition of the present invention exceeds 4, and is close to the isoelectric point of casein. Even in an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, the separation and precipitation of the acidic liquid composition is suppressed, the redispersibility is good, and the form is easy to drink with low viscosity. In addition, when ingested as a muscle synthesis promoter, it can prevent the curding of milk protein in the stomach and promote the absorption of milk protein and enhance the effect of promoting muscle synthesis. The trehalose of the present invention is also commercially available, for example, Treha (produced by Hayashibara).

The trehalose of the present invention is added in an amount of 0.2 to 1.2% by weight of soybean polysaccharide, 0.1 to 0.5% by weight of HM pectin, 0.01% to the acidic liquid composition used in the present invention. In the case of containing 0.1 to 0.1% by weight of fibrous insoluble cellulose, for example, 0.1 to 5% by weight, 0.2 to 4.5% by weight, 0.3 to 4% by weight, 0.4 ~ 3.5 wt%, 0.5 to 3 wt%, 0.6 to 2.5 wt%, 0.7 to 2 wt%, 0.8 to 1.5 wt%, 0.9 to 1.3 % By weight, 0.9 to 1.1% by weight. If the addition amount of the trehalose of the present invention is 5% by weight, the acidic liquid composition of the present invention is not imparted with the flavor and texture derived from trehalose, and it is preferable for achieving a low viscosity and easy to take form. Moreover, even if it is an acidic liquid composition containing the milk protein mixture containing a high concentration casein protein that the addition amount of the trehalose of this invention exceeds 0.01 weight%, the acidic liquid composition used by this invention Separation of substances and occurrence of precipitation can be suppressed, and redispersibility is also improved. Finally, as a muscle synthesis promoter of the present invention, milk protein is not carded in the stomach, and absorption of milk protein is achieved. Is preferable in that the effect of promoting muscle synthesis of the present invention is enhanced.

The method for producing the acidic liquid composition used in the present invention comprises 0.2 to 1.2% by weight of soybean polysaccharide, 0.1 to 0.5% by weight of HM pectin 0.01 to 0.1% by weight. A milk-containing solution containing fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein is characterized in that it comprises a step of adjusting pH to acidity and a step of homogenizing.

In the method for producing an acidic liquid composition used in the present invention, adjusting the pH to acid is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, which is a feature of the present invention. In addition, it suppresses separation and precipitation of the acidic liquid composition, has good redispersibility, has a low viscosity and is easy to drink, and when ingested as a muscle synthesis promoter of the present invention, milk protein in the stomach In the range where an acidic liquid composition capable of enhancing the muscle synthesis promoting effect can be obtained after promoting the absorption of milk protein and preventing absorption of milk protein, the milk-containing solution can be lowered to a predetermined pH, There are no particular restrictions on the means and methods. Methods for adjusting the pH of this milk-containing solution to acid include, for example, a method of fermenting a milk-containing solution with a fermenting microorganism such as lactic acid bacteria, and a method of adding an acid to a milk-containing solution.

In the method for producing an acidic liquid composition used in the present invention, the milk-containing solution contains an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, which is one of the characteristics of the acidic liquid composition of the present invention. Even if it is a product, it suppresses separation and precipitation of the acidic liquid composition, has good redispersibility, has a low viscosity and is easy to drink, and when taken as a muscle synthesis promoter, milk in the stomach 0.2 to 1.2% by weight of soy polysaccharides, 0.1 to 0.5%, in order to prevent the formation of protein curd and promote the absorption of milk protein and enhance the effect of promoting muscle synthesis It is preferable to contain 1% by weight of HM pectin and 0.01 to 0.1% by weight of fibrous insoluble cellulose. In the method for producing an acidic liquid composition of the present invention, the soybean polysaccharide, HM pectin, and fibrous insoluble cellulose contained in the milk-containing solution are added together with some or all of other raw materials, and 50 to 100 are added. You may make it melt | dissolve in the state heated at degreeC, and you may prepare what was melt | dissolved previously with warm water etc. In the method for producing the acidic liquid composition of the present invention, the milk-containing solution is, for example, a stabilizer obtained by dissolving a milk protein mixture in water, soybean polysaccharide, HM pectin, and fibrous insoluble cellulose in water. It is obtained by mixing the liquid.

In the method for producing an acidic liquid composition used in the present invention, in addition to adjusting the pH by adding an acid to the milk-containing solution, the milk-containing solution is mechanically sheared in order to enhance the muscle synthesis promoting effect of the present invention. Homogenization can be achieved by processing. By homogenization, the casein protein of the milk-containing solution whose pH has been adjusted is atomized to previously contain 0.2 to 1.2% by weight of soybean polysaccharide, 0.1 to 0.5% by weight of HM Acid containing pectin and a milk protein mixture containing a high concentration of casein protein, combined with 0.01 to 0.1% by weight of fibrous insoluble cellulose and one of the characteristics of the acidic liquid composition of the present invention Even a liquid composition suppresses separation and precipitation of an acidic liquid composition, has good redispersibility, has a low viscosity and is easy to drink, and when taken as a muscle synthesis promoter, Thus, an acidic liquid composition capable of preventing the curding of milk protein and promoting the absorption of milk protein and enhancing the effect of promoting muscle synthesis can be obtained. For the homogenization, a general-purpose homogenizer used for homogenizing fat globules such as milk and liquefying fermented milk can be used.

In the method for producing an acidic liquid composition used in the present invention, in addition to adjusting the pH by adding an acid to a milk-containing solution, the milk-containing solution is homogenized using a homogenizer in order to enhance the effect of the present invention. The homogenization pressure is, for example, 1 to 100 MPa, 2 to 80 MPa, 3 to 60 MPa, 4 to 40 MPa, 5 to 20 MPa, 6 to 19 MPa, 7 to 18 MPa, 8 to 17 MPa, 9 to 16 MPa, 10 to 15 MPa. The homogenizing temperature is, for example, 50 to 100 ° C., 55 to 95 ° C., 60 to 90 ° C., 65 to 85 ° C., or 70 to 80 ° C. In the method for producing an acidic liquid composition used in the present invention, the step of adjusting the pH by adding an acid to the milk-containing solution and the step of homogenizing the milk-containing solution using a homogenizer are in that order. There is no limit. That is, in the method for producing an acidic liquid composition used in the present invention, it is also possible to homogenize the milk-containing solution using a homogenizer after adjusting the pH by adding an acid to the milk-containing solution. It is also possible to adjust the pH by homogenizing the milk-containing solution using a homogenizer and then adding an acid to the milk-containing solution. In the method for producing an acidic liquid composition used in the present invention, the step of homogenizing treatment is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, which is one of the characteristics of the acidic liquid composition. Even if it is a product, it suppresses separation and precipitation of the acidic liquid composition, has good redispersibility, has a low viscosity and is easy to ingest, and when ingested as a muscle synthesis promoter, It can be set before or after heat sterilization, which will be described later, within a range where an acidic liquid composition is obtained that prevents the formation of milk protein and promotes the absorption of milk protein and enhances the effect of promoting muscle synthesis. it can.

In the method for producing an acidic liquid composition used in the present invention, heat sterilization can be performed in order to enhance the storage stability of the acidic liquid composition. In the method for producing an acidic liquid composition used in the present invention, the method for heat sterilization of the acidic liquid composition may be a known method for sterilizing milk and / or beverage and sterilization conditions. Sterilization methods include, for example, plate-type heat sterilization, tube-type heat sterilization, batch-type heat sterilization, energizing heat sterilization, microwave heat sterilization, indirect heat sterilization such as retort heating, direct injection such as steam injection, steam infusion, etc. Sterilization. The sterilization conditions are the same as for milk, for example, hold at 60 to 65 ° C for 20 to 30 minutes, hold at 80 to 100 ° C for 15 seconds to 10 minutes, hold at 110 ° C to 150 ° C for 2 to 15 seconds, and equivalent If it is sterilization conditions, it can change suitably.

In the method for producing an acidic liquid composition used in the present invention, non-heat sterilization can be performed in order to improve the storage stability of the acidic liquid composition. In the method for producing an acidic liquid composition of the present invention, the non-heat sterilization method of the acidic liquid composition of the present invention can be set with known sterilization methods and sterilization conditions. Non-heat sterilization methods include, for example, light irradiation sterilization, radiation irradiation sterilization, and high voltage pulse sterilization. The sterilization conditions for non-heat sterilization are the same as the heat sterilization of milk, for example, holding at 60 to 65 ° C. for 20 to 30 minutes, holding at 80 to 100 ° C. for 15 seconds to 10 minutes, and 110 to 150 ° C. for 2 to 2 minutes. If the sterilization effect is equivalent to this for 15 seconds, the sterilization conditions for non-heat sterilization can be appropriately changed.

In the method for producing an acidic liquid composition used in the present invention, in order to improve the storage stability of the acidic liquid composition, 0.2 to 1.2% by weight soybean polysaccharide, 0.1 to 0.5% by weight An acidic liquid composition obtained by a step including a step of adjusting pH to an acidity and a step of homogenizing a milk-containing solution containing 0.01 to 0.1% by weight of fibrous HM pectin and insoluble cellulose. Can be cooled. The temperature of the finally cooled acidic liquid composition is, for example, 0 to 30 ° C., 0 to 25 ° C., 0 to 20 ° C., 0 to 15 ° C., 0 to 12 ° C., 0 to 10 ° C.

The acidic liquid composition used in the present invention is an acidic liquid composition containing a milk protein mixture containing a high concentration of casein protein, which is one of the characteristics of the acidic liquid composition of the present invention. Suppresses separation and precipitation of substances, has good redispersibility, has a low viscosity and is easy to drink, and prevents ingestion of milk protein in the stomach when ingested as a muscle synthesis promoter. Other food materials and / or food additive materials can be added if the effect of promoting muscle synthesis can be enhanced after promoting the absorption of the food. For example, for the purpose of imparting the palatability of the acidic liquid composition of the present invention, sweeteners (sugars, liquid sugars, fructose, maltose, trivalent sugar, etc.), sugar alcohols (erythritol, etc.), high-sensitivity sweeteners (stevia) , Aspartame, sucralose, acesulfame potassium), fragrance, fruit juice, vegetable juice, and the like. In addition, for the purpose of imparting the functionality of the acidic liquid composition of the present invention, minerals (calcium, iron, manganese, magnesium, zinc, etc.), vitamins, functional materials, probiotic lactic acid bacteria, etc. are added, The muscle synthesis promoter of the present invention can be used.

The muscle synthesis promoter of the present invention can be in the form of a packaged beverage or a packaged beverage for the purpose of providing in the market. A well-known thing, such as a paper container (a gable top type container, a brick type container, etc.), a soft bag, a plastic bottle, a can, a bottle, can be used for a container. The capacity of the container is not particularly limited, and is, for example, 10 to 5000 g, 20 to 4000 g, 30 to 3000 g, 40 to 2000 g, 50 to 1500 g, 60 to 1400 g, 70 to 1300 g, 80 to 1200 g, 90 to 1100 g. In particular, in the case of a family size (multiple drinks), for example, 300 to 5000 g, 300 to 4000 g, 300 to 3000 g, 300 to 2000 g, 300 to 1500 g, 300 to 1400 g, 300 to 1300 g, 300 to 1200 g, 300 to 1100 g In the case of personal size (individual drinking), it is 10 to 500 g, 20 to 500 g, 30 to 500 g, 40 to 500 g, 50 to 500 g, 60 to 500 g, 70 to 500 g, 80 to 500 g, 90 to 500 g.

The muscle synthesis promoter of the present invention suppresses the separation and precipitation of the acidic liquid composition, even if it is an acidic liquid composition containing milk protein containing a high concentration of casein. Good dispersibility, low viscosity and easy-to-drink form, and as a muscle synthesis promoter, prevents milk protein curd formation in the stomach and promotes absorption of milk protein, and also promotes muscle synthesis. If it can be increased, there is no limit to the form of provision to the market. For example, with the muscle synthesis promoter of the present invention, chilled distribution at 10 ° C. or lower and normal temperature distribution are possible. The muscle synthesis promoter of the present invention also has a heat resistance effect. Therefore, in the composition in which the muscle synthesis promoter of the present invention is blended (added, mixed), the muscle synthesis promoter of the present invention has a heat-resistant effect, so that it can be preferably provided as a liquid product form. At the same time, it can be provided as a product form for warm sales using a hot vendor or the like.

According to one embodiment of the present invention, for example, in the muscle synthesis promoter of the present invention, the milk protein mixture is ingested (administered) so that the total protein amount is 0.06 g / kg body weight per meal, Specifically, the weight is 0.06 to 0.7 g / kg body weight, preferably 0.06 to 0.6 g / kg body weight, more preferably 0.06 to 0.5 g / kg body weight, and still more preferably 0.0. It is ingested (administered) at a dose of 08 to 0.4 g / kg body weight, particularly preferably 0.1 to 0.4 g / kg body weight. Here, the typical weight of the subject is estimated to be about 60 kg.

The term “one meal” means one time in a state that requires immediate effective muscle synthesis promotion. For example, in the muscle synthesis promoter of the present invention, a single meal is not taken at a time (at a time), It may be taken in several degrees. When the muscle synthesis promoting agent of the present invention is ingested (administered) to a subject in need of immediate muscle synthesis promotion, the amino acid concentration in the blood stream can be immediately In terms of improvement, it is preferably within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, more preferably within 30 minutes after the end of exercise, and particularly preferably, Within 5 minutes after the end of exercise.

The method of ingesting (administering) the muscle synthesis promoting agent of the present invention to a subject in need of immediate muscle synthesis promotion is based on oral administration (oral administration), enteral administration, gastric fistula, etc. Can be appropriately selected, but is preferably taken orally.

According to another embodiment of the present invention, for example, the muscle synthesis promoter of the present invention comprises a unit package form per serving, and the milk protein mixture is contained in the unit as the total intake of the protein. And adjusted to 3 g or more, specifically 3 to 40 g, preferably 3 to 20 g, more preferably 4 to 20 g, still more preferably 4 to 15 g, and particularly preferably 5 to 15 g. Do it. Here, “consisting of a unit packaging form per meal” is a form in which the intake amount per meal is determined in advance, for example, as a food that can be taken orally in a specific amount, not only general foods but also beverages (Drinks, etc.), health supplements, health functional foods, supplements, etc. In "unit packaging form per meal", for example, in the case of liquid beverages, gel-like, paste-like, paste-like jelly, powder-like, granule-like, capsule-like, block-like solid foods, etc. A specific amount (dose) can be defined in packaging containers such as cans, glass bottles (such as bottles), plastic containers (such as plastic bottles), packs, pouches, film containers, paper boxes, etc., or intake per serving (usage, dose) ) Is displayed on a packaging container, a homepage, etc., and the form which can prescribe | regulate a specific quantity is mentioned.

According to a preferred embodiment of the present invention, the muscle synthesis promoter of the present invention comprises 0.4 to 0.9% by weight of soybean polysaccharide, 0.2 to 0.25% by weight of HM pectin, 0.03 to 0%. A muscle synthesis promoter comprising, as active ingredients, an acidic liquid composition comprising 0.05% by weight of fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein,
The protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2-6.

According to a more preferred embodiment of the present invention, the muscle synthesis promoter of the present invention comprises 0.4 to 0.9% by weight of soybean polysaccharide, 0.2 to 0.25% by weight of HM pectin, 0.03 to A muscle synthesis promoter comprising as an active ingredient an acidic liquid composition comprising 0.05% by weight of fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein,
The protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2-4.

According to a further preferred embodiment of the present invention, the muscle synthesis promoter of the present invention comprises 0.4 to 0.9% by weight of soybean polysaccharide, 0.2 to 0.25% by weight of HM pectin, 0.03 to Muscle synthesis promotion comprising 0.05% by weight fibrous insoluble cellulose, trehalose 0.9 to 1.1% by weight and an acidic liquid composition containing a milk protein mixture containing casein protein and whey protein as active ingredients An agent,
The protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2-4.

According to one embodiment of the present invention, for example, the muscle synthesis promoter of the present invention can provide a composition comprising a muscle synthesis promoter. That is, the muscle synthesis promoter of the present invention can be used alone as it is, but as long as the muscle synthesis promotion function is exhibited, various compositions for oral intake (for oral administration) such as foods and pharmaceuticals. On the other hand, it can also be included as a raw material (material), an additive, etc., and can obtain the composition which has a muscle synthesis promotion effect. Therefore, according to one embodiment of the present invention, a composition comprising the muscle synthesis promoter of the present invention is provided.

According to one embodiment of the invention, 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous. A composition comprising an acidic liquid composition containing a milk protein mixture containing insoluble cellulose, casein protein and whey protein as an active ingredient, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 A composition for promoting muscle synthesis that is ˜20 can be provided. The composition of the present invention may be a food composition or a pharmaceutical composition.

The food composition is other than the pharmaceutical composition, and is not particularly limited as long as it is a form that can be taken orally, such as a solution, suspension, emulsion, powder, or solid molded product. Specifically, for example, instant noodles, retort food, canned food, microwave food, instant soup / miso soup, freeze-dried food, etc .; soft drink, fruit juice drink, vegetable drink, soy milk drink, coffee drink, tea Beverages such as beverages, powdered beverages, concentrated beverages, alcoholic beverages; flour products such as bread, pasta, noodles, cake mixes, bread crumbs; rice cakes, caramel, chewing gum, chocolate, cookies, biscuits, cakes, pies, snacks, crackers, Confectionery such as Japanese confectionery, dessert confectionery; sauces, tomato processing seasonings, flavor seasonings, cooking mixes, sauces, dressings, soups, curry and stew ingredients; processed fats, butter, margarine, Fats and oils such as mayonnaise; milk drinks, yogurts, lactic acid bacteria drinks, ice creams, creams How dairy products; agricultural canning, jam, marmalade such, processed agricultural products such as serial; such as frozen food, and the like. Preferably, the food is a dairy product, more preferably a milk beverage or a lactic acid bacteria beverage.

Foods are also labeled with health foods, functional foods, dietary supplements, functional labeling foods, foods for specified health use, foods for the sick, infant formulas, powdered milk for pregnant or lactating women, or a disease risk reduction label. Such foods are also included.

According to another embodiment of the invention, 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous. The protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20, comprising as an active ingredient an acidic liquid composition containing a milk protein mixture containing insoluble cellulose, casein protein and whey protein, A food for promoting muscle synthesis is provided. The food for promoting muscle synthesis of the present invention includes foods that display the effect of promoting muscle synthesis or the effects resulting therefrom. According to a preferred embodiment of the present invention, 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous A muscle synthesis promoting beverage (preferably a dairy product, more preferably a dairy beverage or a lactic acid bacteria beverage) comprising an acidic liquid composition containing a milk protein mixture containing insoluble cellulose, casein protein and whey protein as an active ingredient, Provided is a dairy product for promoting muscle synthesis, wherein the ratio of casein protein to protein (casein protein / whey protein) is 2 to 20.

The pharmaceutical composition is prepared as an oral preparation or a parenteral preparation according to a conventional method using additives that are acceptable for formulation. For oral preparations, take the form of solid preparations such as tablets, powders, fine granules, granules, capsules, pills, sustained-release preparations, and liquid preparations such as solutions, suspensions, and emulsions. Can do. In the case of a parenteral preparation, it can take the form of an injection or a suppository. From the viewpoint of simplicity, an oral preparation is preferable. Additives that are acceptable for formulation include, for example, excipients, stabilizers, preservatives, wetting agents, emulsifiers, lubricants, sweeteners, colorants, fragrances, buffers, antioxidants, pH Examples thereof include regulators. The pharmaceutical composition of the present invention comprises a composition for the treatment of diseases requiring the promotion of muscle synthesis, specifically a muscular damage disease such as sarcopenia, more specifically a preventive composition and a therapeutic composition. Is included.

According to yet another embodiment of the present invention, 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fiber. A method for promoting muscle synthesis, comprising: ingesting an acidic liquid composition comprising a milk protein mixture comprising insoluble cellulose, casein protein and whey protein simultaneously or sequentially to a subject in need of muscle synthesis promotion A method for promoting muscle synthesis, in which the ratio of the amount of casein protein to whey protein (casein protein / whey protein) is 2 to 20, is provided. The method for accelerating muscle synthesis of the present invention is preferably a method that excludes medical practice for humans. Here, the “medical action for humans” means an action that requires a doctor or the like to ingest (administer) a medicine to a human. The muscle synthesis promoting method of the present invention can be carried out according to the contents described in the present specification for the muscle synthesis promoting agent of the present invention.

According to one preferred embodiment of the present invention, in the muscle synthesis promotion method of the present invention, the acidic liquid composition may contain 0.5 to 5% by weight of trehalose. According to one preferred embodiment of the present invention, the pH of the acidic liquid composition can be adjusted to 3 to 6 in the muscle synthesis promoting method of the present invention. Furthermore, according to one preferable aspect of the present invention, in the muscle synthesis promoting method of the present invention, the acidic liquid composition may contain fermented cellulose as fibrous unnecessary cellulose.

According to one preferred embodiment of the present invention, in the muscle synthesis promoting method of the present invention, the milk protein mixture is ingested (administered) so that the total protein amount is 0.06 g / kg body weight or more per meal. Specifically, it is 0.06 to 0.7 g / kg body weight, preferably 0.06 to 0.6 g / kg body weight, more preferably 0.06 to 0.5 g / kg body weight, still more preferably It can be ingested so as to give 0.08 to 0.4 g / kg body weight, particularly preferably 0.1 to 0.4 g / kg body weight.

According to one preferred embodiment of the present invention, in the muscle synthesis promotion method of the present invention, the milk protein mixture can be ingested so that the total protein amount is 3 g or more per meal, specifically 3 It can be ingested to a dose of ˜40 g, preferably 3 to 20 g, more preferably 4 to 20 g, even more preferably 4 to 15 g, particularly preferably 5 to 15 g.

According to one preferred embodiment of the present invention, in the muscle synthesis promoting method of the present invention, the subject in need of muscle synthesis promotion is preferably a subject in need of immediate muscle synthesis promotion, more preferably It can be ingested by a subject that exercises, more preferably by a subject performing muscle training.

According to one embodiment of the present invention, in the muscle synthesis promotion method of the present invention, the acidic liquid composition can be ingested in a heated liquid form to a subject in need of muscle synthesis promotion.

According to another embodiment of the present invention, 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous. An insoluble cellulose, an acidic liquid composition comprising a milk protein mixture comprising casein protein and whey protein, ingested by a disease subject in need of muscle synthesis promotion, for a disease in need of muscle synthesis promotion A treatment method is provided, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2-20. The method for treating a disease requiring the promotion of muscle synthesis of the present invention includes a method for treating a muscle damage disease, for example, a method for treating sarcopenia. Treatment methods include prophylactic and therapeutic methods. The treatment method of the present invention can be carried out in accordance with the contents described in the present specification for the muscle synthesis promotion method of the present invention.

According to one embodiment of the present invention, 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01- A muscle synthesis promoter comprising as an active ingredient an acidic liquid composition comprising 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture comprising casein protein and whey protein, or a composition comprising the same There is provided use of a muscle synthesis promoter or a composition comprising the same, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20. According to one preferred embodiment of the invention, the use of the invention is a non-therapeutic use. The use of the muscle synthesis promoter of the present invention or the composition comprising the same can be carried out according to the contents described herein for the muscle synthesis promoter of the present invention and the muscle synthesis promotion method.

According to one embodiment of the present invention, 0.2-1.2% by weight soy polysaccharide, 0.1-0.5% by weight for the manufacture of a food or pharmaceutical product to promote muscle synthesis. A muscle synthesis promoter comprising as an active ingredient an acidic liquid composition comprising HM pectin, 0.01 to 0.1% by weight of fibrous insoluble cellulose, and a milk protein mixture containing casein protein and whey protein, or There is provided a composition comprising the same, a muscle synthesis promoter having a protein content ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20, or a composition comprising the same. The The use of the muscle synthesis promoter or composition of the present invention can be carried out in accordance with the contents described herein for the muscle synthesis promoter and the muscle synthesis promotion method of the present invention.

According to one preferred embodiment of the present invention, in the use of the present invention, the acidic liquid composition can contain 0.5 to 5% by weight of trehalose. According to one preferred embodiment of the present invention, the pH of the acidic liquid composition can be adjusted to 3-6 in the use of the present invention. Furthermore, according to one preferable aspect of the present invention, in the use of the present invention, the acidic liquid composition can contain fermented cellulose as fibrous unnecessary cellulose.

According to one preferred embodiment of the present invention, in the use of the present invention, the milk protein mixture can be ingested (administered) so that the total protein amount is 0.06 g / kg body weight or more per meal, Specifically, it is 0.06 to 0.7 g / kg body weight, preferably 0.06 to 0.6 g / kg body weight, more preferably 0.06 to 0.5 g / kg body weight, and still more preferably 0.08. It can be ingested at a dose of ˜0.4 g / kg body weight, particularly preferably 0.1-0.4 g / kg body weight.

According to one preferred embodiment of the present invention, in the use of the present invention, the milk protein mixture can be ingested so that the total protein amount is 3 g or more per meal, specifically, 3 to 40 g, The ingestion amount is preferably 3 to 20 g, more preferably 4 to 20 g, still more preferably 4 to 15 g, and particularly preferably 5 to 15 g.

According to one preferred embodiment of the present invention, in the use of the present invention, the subject is an exercise subject, preferably a muscle training subject.

According to one embodiment of the invention, 0.2-1.2% by weight soybean polysaccharide, 0.1-0.5% by weight HM pectin, 0.01-0.1% by weight fibrous. An insoluble cellulose and an acidic liquid composition comprising a milk protein mixture containing casein protein and whey protein, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20 An acidic liquid composition is provided to facilitate synthesis. The acidic liquid composition for promoting muscle synthesis of the present invention can be designed according to the contents described herein for the muscle synthesis promoter of the present invention.

By the way, the muscle synthesis promoter of the present invention is, for example, 0.2 to 1.2% by weight soybean polysaccharide, 0.1 to 0.5% by weight HM pectin, 0.01 to 0.1% by weight. A muscle synthesis promoter comprising a milk protein mixture containing casein protein and whey protein, which is free of fibrous insoluble cellulose and is not acidified, as an active ingredient, and a casein protein for whey protein The protein synthesis ratio (casein protein / whey protein) is 4, a muscle synthesis promoter based on the knowledge of Non-Patent Document 1 (hereinafter referred to as “muscle synthesis containing a neutral liquid composition consisting only of a milk protein mixture as an active ingredient” Compared with the “accelerating agent”), the muscle synthesis promoting effect is further enhanced. For example, when the same amount of milk protein is ingested (administered) in an Example (Test Example 1) based on animal experiments described later, the muscle synthesis promoter of the present invention is a neutral liquid composition consisting only of a milk protein mixture. Compared with the muscle synthesis promoter containing as an active ingredient, it was shown to have a high effect. Further, in Examples (Test Example 2) based on animal experiments described later, the muscle synthesis promoter of the present invention was ingested (administered) at a low dose of 0.85 g milk protein / kg body weight, and consisted only of milk protein mixture When a muscle synthesis promoter containing a neutral liquid composition as an active ingredient is ingested (administered) at a high dose of 1.8 g milk protein / kg body weight, the effect as a muscle synthesis promoter is comparable. It was.

That is, the muscle synthesis promoter of the present invention is stronger when a certain amount of milk protein is ingested (administered) compared to a muscle synthesis promoter comprising a neutral liquid composition consisting only of a milk protein mixture as an active ingredient. Expected to promote muscle synthesis. In addition, the muscle synthesis promoter of the present invention reduces the amount of milk protein mixture to be consumed in order to obtain the same effect as the muscle synthesis promoter comprising a neutral liquid composition consisting only of a milk protein mixture as an active ingredient. Can be more easily taken (administered). Furthermore, the muscle synthesis promoter of the present invention has a refreshing property with a moderate acidity, and has the same concentration of milk protein as compared to a muscle synthesis promoter comprising a neutral liquid composition consisting only of a milk protein mixture as an active ingredient. Even when it contains a mixture, it becomes easier to ingest (administer).

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

[Example 1]
4.25 kg of milk protein concentrate (milk protein 80 wt%, lactose 6.5 wt%) was dissolved by heating in 40.75 kg of water to obtain a solution A. Soy polysaccharides (SM-900, Saneigen FFI) 0.45 kg, HM pectin (AYD5110SB, Unitech Foods) 0.2 kg, fermented cellulose (Sun artist PG, Saneigen FFI) 0.05 kg 1 kg of trehalose and 3.3 kg of sugar 0.0063 kg of sucralose were dissolved by heating in 39.99 kg of water to obtain a solution B. A solution C was prepared by dissolving 0.64 kg of citric acid in 9.36 kg of water. After mixing the solution A and the solution B, the solution C was added in 10 portions over 5 minutes while stirring the mixed solution to obtain a base mix before sterilization. The base mix was homogenized at 15 MPa (70 to 80 ° C.) with a plate sterilizer, sterilized at 130 ° C. for 2 seconds, and then cooled to 10 ° C. or lower to obtain the muscle synthesis promoter of Example 1. (3.4% by weight of milk protein, 0.45% by weight of soybean polysaccharide, 0.2% by weight of HM pectin, 0.05% by weight of fermented cellulose, 1% by weight of trehalose). The muscle synthesis promoter of Example 1 had a pH of 4.1 and a viscosity of 10 mPa · s (10 ° C.). “Drinking mouth”, “precipitation” and “redispersibility” were all good. Hereinafter, in Test Examples 1 and 2, Example 1 is referred to as “muscle synthesis promoter”. The milk protein concentrate used here is casein protein obtained by subjecting skim milk to membrane separation treatment (UF treatment, MF treatment, etc.), removing lactose and ash, and concentrating mainly on casein protein and whey protein. And a whey protein in the form of a dry powder. And the protein amount ratio of milk protein concentrate is casein protein: whey protein = about 8: 2, like raw milk (milk) (see Kunio Yamauchi and Kenkichi Yokoyama, Milk General Dictionary).

[Test Example 1]
The muscle synthesis promoting effect of the muscle synthesis promoter of Example 1 was verified as follows.

(1) Rat swimming test SD male rats (obtained from CLEA Japan, Inc.) having an initial weight of 130 to 170 g were used as experimental animals. All rats were individually housed in stainless steel cages at room temperature of 22 ± 2 ° C. with a light period of 7 to 19 o'clock and a dark period of 19 to 7 o'clock.

The rats were preliminarily raised for 3 to 5 days using a solid feed (Oriental Yeast Co., Ltd.), and then subjected to a 30-minute swimming exercise (water temperature 34 ° C.) in order to acclimate to the swimming exercise. After fasting for 16 hours from the next day to the day after the acclimated swimming exercise, the rats were divided into 9 groups (6 to 8 animals in each test group) so that there was no difference in body weight between the test groups. Thereafter, the rats were subjected to a 2-hour swimming exercise. Immediately after the end of swimming exercise, rats were orally ingested with a muscle synthesis promoter or a skim milk solution of 3.4% by weight of milk protein at 24.24 mL / kg body weight. After 15 minutes, 45 minutes, 75 minutes, or 105 minutes after ingestion, a deuterium labeled phenylalanine solution (prepared by dissolving 225 mg of deuterium labeled phenylalanine in 10 mL of physiological saline) was added at 2.0 mL / Rats were administered tail vein with kg body weight. Rats were dissected under isoflurane anesthesia 15 minutes after tail vein administration (ie 30 minutes, 60 minutes, 90 minutes, or 120 minutes after oral ingestion). After whole blood was collected from the abdominal vena cava, the triceps muscle of the upper limb was removed, washed with physiological saline, and immediately frozen with liquid nitrogen. The time of tail vein administration and the time of cryopreservation of the upper limb triceps were accurately recorded. The upper limb triceps were stored at −80 ° C. until analysis.

(2) Measurement of skeletal muscle synthesis rate (FSR) The triceps muscle of the extremity extracted and stored based on the description in the previous literature (A. Kanda et al., Br J Nutr, Sep. 28, 981-987, 2013) The skeletal muscle synthesis rate (FSR) for was measured. Specifically, the amounts of phenylalanine (Phe) and deuterium labeled phenylalanine (Phe (Ring-D5)) that are assimilated to the protein of skeletal muscle cells or are present in the skeletal muscle cells in a free state are expressed as LC Quantification was performed using / MS / MS (manufactured by Waters). The measurement conditions are as follows. That is, apparatus: LC / MS / MS (manufactured by Waters), column: ACQUITY UPLC BEH C18 (manufactured by Waters), mobile phase: acetonitrile containing 0.05% TFA water / 0.05% TFA, injection amount 3 μL, flow rate 0.3 mL / min, UV 215 nm.

Here, the skeletal muscle synthesis rate (FSR) was calculated by the following equation.
FSR (% / day) = (Eb × 100) / (Ea × t)
Ea: enrichment (present in free state in skeletal muscle cells)
Eb: enrichment (present assimilated to skeletal muscle cell protein)
t: Time from administration of tail vein to cryopreservation of triceps surae (unit: days)
Enrichment = Phe (Ring−D5) / (Phe + Phe (Ring−D5))

The average value ± standard error of each test group was determined from the obtained numerical values. In statistical processing, Student's t-test was performed between the muscle synthesis promoter group and the skim milk solution group at each time point (30 minutes, 60 minutes, 90 minutes, or 120 minutes after oral intake). The significance level was less than 5% (p <0.05). The result is shown in FIG.

As a result of ingestion of the muscle synthesis promoter, the skeletal muscle synthesis rate of the rat 30 minutes after ingestion was significantly higher than that of the skim milk solution (p <0.05, FIG. 1). In addition, the intake of the muscle synthesis promoter tended to increase the value of the skeletal muscle synthesis rate even after 60 minutes, 90 minutes, and 120 minutes of intake compared to the skim milk solution (p <0.10, FIG. 1). From these results, it was shown that the muscle synthesis promoter has a higher skeletal muscle synthesis promoting effect than the skim milk powder solution.

[Test Example 2]
The muscle synthesis promoting effect of the muscle synthesis promoter of Example 1 was verified as follows.

(1) Rat swimming test SD male rats (obtained from CLEA Japan, Inc.) having an initial weight of 130 to 170 g were used as experimental animals. All rats were individually housed in stainless steel cages at room temperature of 22 ± 2 ° C. with a light period of 7 to 19 o'clock and a dark period of 19 to 7 o'clock.

The rats were preliminarily raised for 3 to 5 days using a solid feed (Oriental Yeast Co., Ltd.), and then subjected to a 30-minute swimming exercise (water temperature 34 ° C.) in order to acclimate to the swimming exercise. After fasting for 16 hours from the next day to the day after the acclimated swimming exercise, the rats were divided into 9 groups (6 to 8 animals in each test group) so that there was no difference in body weight between the test groups. Thereafter, the rats were subjected to a 2-hour swimming exercise. Immediately after the end of swimming exercise, rats were orally ingested with a muscle synthesis promoter or 6.8 wt% skim milk solution (high-dose skim milk solution) of milk protein at 24.24 mL / kg body weight. After 15 minutes, 45 minutes, 75 minutes, or 105 minutes after ingestion, a deuterium labeled phenylalanine solution (prepared by dissolving 225 mg of deuterium labeled phenylalanine in 10 mL of physiological saline) was added at 2.0 mL / Rats were administered tail vein with kg body weight. Rats were dissected under isoflurane anesthesia 15 minutes after tail vein administration (ie 30 minutes, 60 minutes, 90 minutes, or 120 minutes after oral ingestion). After whole blood was collected from the abdominal vena cava, the triceps muscle of the upper limb was removed, washed with physiological saline, and immediately frozen with liquid nitrogen. The time of tail vein administration and the time of cryopreservation of the upper limb triceps were accurately recorded. The upper limb triceps were stored at −80 ° C. until analysis.

(2) Measurement of skeletal muscle synthesis rate (FSR) The triceps muscle of the extremity extracted and stored based on the description in the previous literature (A. Kanda et al., Br J Nutr, Sep. 28, 981-987, 2013) The skeletal muscle synthesis rate (FSR) for was measured. Specifically, the amounts of phenylalanine (Phe) and deuterium labeled phenylalanine (Phe (Ring-D5)) that are assimilated to the protein of skeletal muscle cells or are present in the skeletal muscle cells in a free state are expressed as LC Quantification was performed using / MS / MS (manufactured by Waters). The measurement conditions are as follows. That is, apparatus: LC / MS / MS (manufactured by Waters), column: ACQUITY UPLC BEH C18 (manufactured by Waters), mobile phase: acetonitrile containing 0.05% TFA water / 0.05% TFA, injection amount 3 μL, flow rate 0.3 mL / min, UV 215 nm.

Here, the skeletal muscle synthesis rate (FSR) was calculated by the following equation.
FSR (% / day) = (Eb × 100) / (Ea × t)
Ea: enrichment (present in free state in skeletal muscle cells)
Eb: enrichment (present assimilated to skeletal muscle cell protein)
t: Time from administration of tail vein to cryopreservation of triceps surae (unit: days)
Enrichment = Phe (Ring−D5) / (Phe + Phe (Ring−D5))

The average value ± standard error of each test group was determined from the obtained numerical values. In statistical processing, Student's t-test was performed between the muscle synthesis promoter group and the skim milk solution group at each time point (30 minutes, 60 minutes, 90 minutes, or 120 minutes after oral intake). The significance level was less than 5% (p <0.05). The result is shown in FIG.

The muscle synthesis promoter showed an effect of promoting skeletal muscle synthesis equivalent to that of a high-dose skim milk solution. The intake of milk protein as the active body is 0.85 g / kg body weight in the muscle synthesis promoter group, and 1.8 g / kg body weight in the high-dose skim milk solution group. It was also shown that skeletal muscle synthesis was promoted with low milk protein intake.

[Test Example 3]
The artificial gastric juice (containing 0.6% pepsin, pH 1.2) and the muscle synthesis promoter were mixed so that the weight ratio (artificial gastric fluid) :( muscle synthesis promoter) = 1: 1.6, 37 Hold for 10 minutes. At this time, in the muscle synthesis promoter mixed with the artificial gastric juice, milk proteins derived from the muscle synthesis promoter did not aggregate. As a control, the same test was carried out by using the milk protein 3.4% by weight skim milk solution used in Test Example 1 and the milk protein 6.8% by weight skim milk powder solution used in Test Example 2 (high-dose skim milk solution). A test using was also performed. Artificial gastric juice (containing 0.6% pepsin, pH 1.2) and skim milk powder solution are mixed at a weight ratio of (artificial gastric fluid) :( skim milk powder solution) = 1: 1.6 at 37 ° C. Hold for a minute. At this time, in the skim milk powder solution mixed with the artificial gastric juice, milk protein derived from the skim milk powder solution aggregated.

From Test Examples 1 to 3, the muscle synthesis promoter of the present invention is not aggregated (carded) in the stomach after ingestion (administration) as compared with the skim milk solution, and the subsequent intestinal tract It was inferred that the milk protein absorbability increased in, and the effect of promoting muscle synthesis was enhanced.

Claims (13)

0.2-1.2% by weight of soy polysaccharides,
0.1-0.5% by weight of HM pectin,
A muscle synthesis promoter comprising as an active ingredient an acidic liquid composition comprising 0.01 to 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein,
A muscle synthesis promoter having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20. The muscle synthesis promoter according to claim 1, wherein the acidic liquid composition further comprises 0.5 to 5% by weight of trehalose. 3. The muscle synthesis promoter according to claim 1 or 2, wherein the acidic liquid composition has a pH of 3 to 6. The muscle synthesis promoter according to any one of claims 1 to 3, wherein the fibrous insoluble cellulose is fermented cellulose. The muscle synthesis according to any one of claims 1 to 4, wherein the milk protein mixture contained in the acidic liquid composition is ingested so that the total protein amount is 0.06 to 0.7 g / kg body weight per serving. Accelerator. The muscle synthesis promotion according to any one of claims 1 to 5, comprising a unit packaging form per serving, wherein the unit packaging form contains 3 to 40 g of the total amount of protein as a meal intake of a milk protein mixture. Agent. The muscle synthesis promoter according to any one of claims 1 to 6, for a subject to exercise. The muscle synthesis promoter according to claim 7, wherein the exercise is muscle training. 0.2-1.2% by weight of soy polysaccharides,
0.1-0.5% by weight of HM pectin,
Ingesting an acidic liquid composition comprising 0.01 to 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture comprising casein protein and whey protein into a subject in need of muscle synthesis promotion. A method for promoting muscle synthesis, wherein the ratio of the amount of casein protein to whey protein (casein protein / whey protein) is 2 to 20. 0.2-1.2% by weight of soy polysaccharides,
0.1-0.5% by weight of HM pectin,
Ingesting an acidic liquid composition comprising 0.01 to 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture comprising casein protein and whey protein into a subject in need of muscle synthesis promotion. A method for promoting muscle synthesis, which comprises a method for promoting muscle synthesis (excluding medical practice for humans), wherein the ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20. To promote muscle synthesis,
0.2-1.2% by weight of soy polysaccharides,
0.1-0.5% by weight of HM pectin,
Use of a muscle synthesis promoter comprising as an active ingredient an acidic liquid composition comprising 0.01 to 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein, ,
Use, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2-20. For the production of food or pharmaceuticals to promote muscle synthesis,
0.2-1.2% by weight of soy polysaccharides,
0.1-0.5% by weight of HM pectin,
Use of a muscle synthesis promoter comprising as an active ingredient an acidic liquid composition comprising 0.01 to 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture containing casein protein and whey protein, ,
Use, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2-20. 0.2-1.2% by weight of soy polysaccharides,
0.1-0.5% by weight of HM pectin,
An acidic liquid composition comprising 0.01 to 0.1% by weight of fibrous insoluble cellulose and a milk protein mixture comprising casein protein and whey protein,
The protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20,
An acidic liquid composition for promoting muscle synthesis.

Images