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WO2016165975A1 - Procédé pour la réalisation continue de réactions enzymatiques sur un substrat organique - Google Patents

Procédé pour la réalisation continue de réactions enzymatiques sur un substrat organique Download PDF

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Publication number
WO2016165975A1
WO2016165975A1 PCT/EP2016/057402 EP2016057402W WO2016165975A1 WO 2016165975 A1 WO2016165975 A1 WO 2016165975A1 EP 2016057402 W EP2016057402 W EP 2016057402W WO 2016165975 A1 WO2016165975 A1 WO 2016165975A1
Authority
WO
WIPO (PCT)
Prior art keywords
turbo
reactor
enzyme
rotor
organic substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2016/057402
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English (en)
Inventor
Massimo VEZZANI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ambiente e Nutrizione SRL
Original Assignee
Ambiente e Nutrizione SRL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambiente e Nutrizione SRL filed Critical Ambiente e Nutrizione SRL
Publication of WO2016165975A1 publication Critical patent/WO2016165975A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes

Definitions

  • the present invention relates to the sector of the chemical industry in general and in particular the chemical industry which uses enzymatic reactions.
  • the present invention relates to a process and a system for carrying out enzymatic reactions intended to obtain products of interest for nutrition or animal husbandry.
  • lignocellulosic biomass may not constitute a food source for monogastric animals, such as pigs, chickens and turkeys, since these animals, differently from plant-eating animals, are unable to digest and convert into glucose lignocellulosic materials such as agricultural waste, owing to the lack of enzymes needed to convert the cellulose and the hemicellulose into glucose.
  • the patent application WO 2014/202716 has recently proposed a process for the production of an additive composition for animal feed from lignocellulosic biomass, comprising a physical, chemical or biological biomass pre-treatment step, such as to allow the subsequent incubation for 3- 120 hours of the biomass pretreated with enzymes having an endoglucanase, beta-glucosidase and endoxylanase activity and substantially without a beta-xylosidase and/ or alpha-L- arabinofuranosidase activity, followed by drying and, where necessary, packaging.
  • composition thus obtained is suitable also for the feeding of monogastric animals.
  • the aforementioned pre-treatment step may consist for example of a dry or wet grinding step, a treatment using high-pressure and high- temperature saturated steam ("steam explosion"), hydro thermolysis, wet oxidation, ammonia fibre explosion (AFEX), a treatment using solvents, ammonia percolation, an acid or alkali treatment, a treatment using microorganisms able to degrade the lignin and the cellulose, such as certain fungi which produce lignocellulolytic enzymes or others which produce hydrogen peroxide.
  • steam explosion high-pressure and high- temperature saturated steam
  • AFEX ammonia fibre explosion
  • This pre-treatment step involves further costs and longer times, in addition to those of the following enzymatic treatment, as well as the possible permanence in the final product of the chemical substances or microorganisms used for the pre-treatment.
  • the problem underlying the present invention is that of providing a process for continuously carrying out enzymatic reactions on organic substrates, without the need for any pre-treatment of these organic substrates.
  • the problem has been solved by providing a process for continuously carrying out enzymatic reactions on an organic substrate, which comprises the steps of: a) providing a turbo-reactor comprising a cylindrical tubular body having at least one opening for the introduction of reagents and at least one discharge opening, an optional heating or cooling jacket for adjusting the temperature of said tubular body to a predetermined temperature, and a rotor, arranged inside the cylindrical tubular body and comprising a shaft provided with elements projecting radially therefrom, and feeding a continuous flow of said organic substrate into said turbo-reactor, in which the rotor is rotated at a speed greater than or equal to 150 rpm, in order to disperse said continuous flow of organic substrate into a flow of particles of said organic substrate, b) feeding into said turbo-reactor, together with said flow of organic substrate, a continuous flow of an aqueous solution of at least one enzyme adapted to convert said organic substrate into a desired product, which is dispersed by said rotor into a flow of minute droplets,
  • the aforementioned thermal inactivation step i) is carried out by means of a turbo-inactivator, comprising a cylindrical tubular body having at least one inlet opening and at least one discharge opening, a heating jacket for adjusting the temperature of said tubular body to a predetermined temperature, and a rotor, arranged inside the cylindrical tubular body and comprising a shaft provided with elements projecting radially therefrom, - feeding said continuous flow of said product and said at least one enzyme exiting said maturation reactor into said turbo-inactivator, through said at least one inlet opening, the inner wall of the turbo- inactivator being kept at a temperature of at least 70°C by means of said heating jacket and the rotor being rotated at a speed of at least 150 rpm; - centrifuging and causing said continuous flow to advance inside the turbo-inactivator through the action of said rotor, and
  • the heating or cooling jacket of the turbo-reactor of step a) is generally intended to be passed through by a heating/ cooling fluid, such as diathermic oil or steam.
  • the aforementioned elements projecting radially from the shaft of the rotor may be for example rod-like or in the form of blades, V-blades or beaters.
  • the aforementioned organic substrate is a lignocellulosic biomass, preferably selected from the group comprising corn cobs or husks; corn, wheat, barley, sorghum, soybean and oat straw; grass, beet pulp; rice, corn or wheat bran; rice hulls, citrus peels, palm kernel, sorghum and sugar cane bagasse, miscanthus, switchgrass, cord grass, sawdust, roots, leaves, wood chips, fruits, flowers, corn fiber, cotton, lignin.
  • lignocellulosic biomass preferably selected from the group comprising corn cobs or husks; corn, wheat, barley, sorghum, soybean and oat straw; grass, beet pulp; rice, corn or wheat bran; rice hulls, citrus peels, palm kernel, sorghum and sugar cane bagasse, miscanthus, switchgrass, cord grass, sawdust, roots, leaves, wood chips, fruits, flowers, corn fiber, cotton, lignin.
  • the aforementioned at least one enzyme is preferably selected from the group comprising endoglucanase, ⁇ -glucosidase, endoxylanase, cellulase - in particular cellobiohydrolase I and cellobiohydrolase II -, pectase, polysaccharide monooxygenase and mixtures thereof.
  • the product obtained with the process according to the present invention may be used as a component of feed for animals, including monogastric animals.
  • the temperature inside the turbo-reactor used in steps a) to e) is kept at a value comprised between 40°C and 70°C, preferably between 45°C and 55°C, by means of the aforementioned heating/cooling jacket.
  • the pH of said solution containing at least one enzyme is adjusted to a value comprised between 5 and 6, preferably between 5.5 and 5.7.
  • the ratio between the flow rate of the organic substrate and the flow rate of said at least one enzyme is comprised between 1 :0.5 and 1 :3.
  • the concentration of said at least one enzyme in the aforementioned aqueous solution is generally comprised between 0.001% and 0.1% w/v, preferably between 0.005 and 0.03% w/v.
  • the residence time of the flows of organic substrate and of said at least one enzyme inside the turbo-reactor is generally comprised between 2 and 15 minutes, preferably between 5 and 10 minutes.
  • the rotational speed of the rotor of the turbo-reactor is generally comprised between 150 and 1000 rpm.
  • the residence time of the flow of said mixture in said steps f) and g) inside the maturation reactor is generally comprised between 0.5 and 10 hours, preferably 0.8-5 hours, conveniently 1-2 hours.
  • the temperature inside the maturation reactor is preferably kept in a range of 40°-70°C, conveniently between 45°C and 55°C, by means of suitable heat exchange means inside or outside the reactor.
  • the thermal treatment of the aforementioned step i) is preferably carried out at a temperature comprised between 70°C and 120°C, conveniently between 90°C and 100°C.
  • the rotational speed of the rotor of the turbo-inactivator is generally between 150 and 1000 rpm.
  • the present invention relates to a system for carrying out the process described above for the conversion of an organic substrate into a product by means of at least one enzyme, comprising the following apparatuses:
  • turbo-reactor comprising a cylindrical tubular body with horizontal or vertical axis, having at least one opening for the introduction of respective continuous flows of said organic substrate and an aqueous solution of said at least one enzyme and at least one discharge opening, an optional heating or cooling jacket for adjusting the temperature of said tubular body to a predetermined temperature, and a rotor, arranged inside the cylindrical tubular body and comprising a shaft which is provided with elements projecting radially therefrom and is able to be rotated at a speed greater than or equal to 150 rpm, and
  • a maturation reactor comprising a box-shaped body, having a bottom, a cover and side walls and provided with at least one inlet opening and at least one discharge opening arranged opposite said inlet opening, a screw- type rotor arranged on the bottom of said box- shaped body, and elements in the form of V-blades and/ or beaters attached to said side walls in the region of said bottom.
  • the system according to the present invention further comprises: - a turbo-inactivator, comprising a cylindrical tubular body with horizontal or vertical axis, having at least one inlet opening and at least one discharge opening, a heating jacket, for adjusting the temperature of said tubular body to a predetermined temperature, and a rotor, arranged inside the cylindrical tubular body and comprising a shaft which is provided with elements projecting radially therefrom and is able to be rotated at a speed of at least 150 rpm.
  • the aforementioned heating or cooling jacket of the turbo-reactor is intended to be passed through by a heating fluid or cooling fluid, preferably consisting of diathermic oil or steam.
  • the aforementioned heating or cooling jacket of the turbo-inactivator is intended to be passed through by a heating fluid, preferably consisting of diathermic oil or steam.
  • the aforementioned elements projecting radially from the shaft of the rotor of the turbo-reactor and of the turbo-inactivator may be rod-like or in the form of blades, V-blades or beaters.
  • the process for enzymatic conversion of organic substrates according to the present invention does not require any preliminary treatment of the organic starting substrate, with a consequent marked reduction in the production times and costs.
  • the intense mechanical action exerted by the rotor of the turbo-reactor used in steps a)-d) is such that a significant quantity of kinetic energy is transmitted to the substrate and enzyme flows such as to trigger rapidly the enzymatic reaction.
  • This triggering step generally does not require more than ten minutes and the creation of the dynamic, thin, tubular layer is able to avoid the negative effects observed in many processes of the prior art, due to the increase in the viscosity of the organic substrate as the reaction proceeds.
  • an apparatus used for the process according to the invention comprises a turbo-reactor T consisting essentially of a cylindrical tubular body 1 , closed at the opposite ends by end plates 2, 3 and coaxially provided with a heating (or cooling) jacket 4 intended to be passed through by a fluid, for example diathermic oil, so as to keep the inner wall of the body 1 at a predefined temperature.
  • the tubular body 1 is provided with inlet openings 5, 6 respectively for the organic substrate and the aqueous solution of at least one enzyme, and with a discharge opening 7.
  • the tubular body 1 rotatably supports internally a rotor comprising a shaft 8 provided with elements 9 radially projecting therefrom in the form of blades, these blades 9 being arranged helically and oriented so as to centrifuge and at the same time convey towards the discharge outlet 7 the flows of organic substrate and aqueous solution of at least one enzyme and the mixture resulting from mixing and the initial reaction between the aforementioned two flows.
  • a motor M is envisaged for operation of the bladed rotor at variable speeds ranging from 150 to 1500 rpm.
  • the intense mechanical action exerted by the blades of the rotor results in the creation of a dynamic, tubular, thin layer which advances gradually along the inner wall of the tubular body, producing on the inside thereof optimum wetting of the substrate by the aqueous solution of the at least one enzyme with triggering of the reaction catalyzed by the latter, while the temperature is kept constantly at the operating temperature of the enzyme by means of the heating/ cooling jacket 4.
  • the aforementioned wet mixture is fed via a pipe 1 1 , communicating with the discharge opening 7 of the turbo-reactor, to the maturation reactor M.
  • the latter comprises a box-shaped body 12, provided with a bottom 13, cover 14 and side walls 15 and provided with at least one inlet opening 16 and at least one discharge opening 17 arranged in a position opposite to that of said inlet opening, close to the bottom, and with a screw-type rotor 18 arranged on the bottom of said box- shaped body and with elements 19 in the form of beaters and/ or V-blades attached to said bottom 13 and to said side walls 15 in the region of said bottom.
  • the screw- type rotor 18, which is rotatably supported close to the bottom 13, ensures, as a result of its rotation and the interaction between the screw and the elements 19 in the form of V-blades and/ or beaters, a continuous slow mixing of the mixture so as to allow continuous renewal of the enzymatic attack surface of the substrate.
  • the rotor also causes the mixture to advance slowly towards the discharge opening 17.
  • a flow of mixture containing the product obtained from the reaction of the at least one enzyme with the organic substrate and the at least one enzyme is continuously discharged from the discharge opening 17 of the maturation reactor M.
  • This flow of mixture is continuously fed into the turbo-inactivator T, through the inlet opening 105.
  • turbo-inactivator T' which has a structure entirely similar to that of the aforementioned turbo-reactor T, is not described in detail.
  • the components of the turbo-inactivator T' which are the same as those of the turbo-reactor T are indicated by the same reference numbers increased by 100.
  • the flow of mixture entering the turbo-inactivator T' is centrifuged by the blades 109 of the rotor against the inner wall of the cylindrical tubular body 101 , heated by means of the heating jacket 104 to a temperature of at least 70°C, preferably between 70°C and 120°C, conveniently between 90°C and 100°C.
  • the rotation of the shaft 108 of the bladed rotor at at least 150 rpm, preferably between 150 and 1000 rpm, has the effect that a dynamic, thin, tubular layer of the aforementioned mixture is created against the heated inner wall of the cylindrical tubular body 101 and an intense heat exchange takes place between the product and enzyme mixture and the aforementioned inner wall. In this way complete thermal inactivation of the enzyme occurs and the product output from the discharge opening 107 of the turbo-inactivator is completely harmless and stable during any following processing stages.
  • the continuous flow of product output from the turbo-inactivator may be collected inside a container 20 designed to separate it from the water vapour also output from the turbo-inactivator.
  • a flow of beet pulp (100 kg/h) was continuously fed, through the opening 6, into the turbo-reactor T, inside which the bladed rotor 8 was rotated at a speed of 700 rpm.
  • a flow (100 1/h) of an aqueous solution of an enzymatic mixture of cellulase and pectinase in a weight ratio of 10: 1 was continuously fed through the opening 5.
  • the concentration of the aqueous solution of enzymatic mixture was equal to 0.01% w/v.
  • the temperature of the inner wall was kept at about 55°C.
  • the temperature was kept at a controlled value of about 55°C while the rotational speed of the screw-type rotor was adjusted so as to cause discharging of the flow of mixture from the opening 17 after an average residence time of about 1 hour.
  • the wall temperature was kept at a controlled value of about 100°C, while the rotational speed of the bladed rotor 108 was kept constantly at 1000 rpm.
  • the mixture was immediately dispersed and centrifuged against the inner wall of the turbo-inactivator T in the form of a dynamic, tubular, thin layer and made to advance towards the discharge opening 107.
  • the inner wall of the turbo-inactivator T in the form of a dynamic, tubular, thin layer and made to advance towards the discharge opening 107.
  • an intense heat exchange occurs between the thin layer of mixture and the heated wall, this resulting in total inactivation of the enzymes present in the mixture.
  • the product output from the turbo-inactivator finally was conveyed into the collection container 20 inside which the water vapour was also removed.
  • the product thus obtained was perfectly safe from a toxicological and microbiological point of view and also perfectly suitable for the nutrition of monogastric animals such as pigs and chickens.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Genetics & Genomics (AREA)
  • Birds (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé pour la réalisation continue de réactions enzymatiques sur un substrat organique, qui comprend les étapes consistant à : • a) utiliser un turboréacteur (T) comprenant un corps tubulaire cylindrique (1) présentant des ouvertures d'entrée (5, 6) et une ouverture d'évacuation (7) et un rotor, comprenant un arbre (8) pourvu d'éléments (9) radialement en saillie, et introduisant un flux continu du substrat organique dans le turboréacteur, • b) introduire dans le turboréacteur, conjointement avec le flux de substrat organique, un flux continu d'une solution aqueuse d'une enzyme, •c) centrifuger les particules et les gouttelettes contre la paroi interne dudit turboréacteur, avec formation d'une mince couche tubulaire de fluide ; • d) démarrer la conversion du substrat organique en le produit désiré au moyen de l'enzyme dans la couche mince ; • e) évacuer, à partir de cette ouverture, un flux continu d'un mélange comprenant le produit, l'enzyme et le substrat organique qui n'a pas encore réagi ; • f) introduire ce flux continu dans un réacteur (M) de maturation, comprenant un corps (12) en forme de boîte, pourvu d'un rotor (18) de type à vis ; • g) soumettre ce flux continu à un mélange continu ; • h) évacuer, du réacteur (M), un flux continu de produit et d'enzyme ; • i) soumettre ce flux continu à un traitement thermique à une température d'au moins 70°C, de manière à inactiver l'enzyme.
PCT/EP2016/057402 2015-04-16 2016-04-05 Procédé pour la réalisation continue de réactions enzymatiques sur un substrat organique Ceased WO2016165975A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMI2015A000557A ITMI20150557A1 (it) 2015-04-16 2015-04-16 Procedimento per eseguire in continuo reazioni enzimatiche su un substrato organico
ITMI2015A000557(102015902344634) 2015-04-16

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WO2016165975A1 true WO2016165975A1 (fr) 2016-10-20

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PCT/EP2016/057402 Ceased WO2016165975A1 (fr) 2015-04-16 2016-04-05 Procédé pour la réalisation continue de réactions enzymatiques sur un substrat organique

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IT (1) ITMI20150557A1 (fr)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110604487A (zh) * 2018-06-15 2019-12-24 广东美的生活电器制造有限公司 灭酶装置和食物料理机
EP3858969A1 (fr) * 2020-02-03 2021-08-04 Sekab E-Technology AB Agencement de prétraitement comprenant un dispositif de raclage

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0231825A2 (fr) * 1986-02-04 1987-08-12 WALTER, Ulrich Procédé et installation de préparation de nourriture pour animaux ou de produits alimentaires
EP0710670A1 (fr) * 1994-11-03 1996-05-08 VOMM IMPIANTI E PROCESSI S.r.L. Procédé pour modifier l'amidon
US20040076715A1 (en) * 2002-10-16 2004-04-22 Mukund Parthasarathy Increased density pet food product and method of production
EP1847621A1 (fr) * 2006-04-21 2007-10-24 Vomm Impianti e Processi S.P.A. Procédé et installation destinés à la production de sucre fermentable à partir de matières contenant de la cellulose
EP2620462A1 (fr) * 2012-01-24 2013-07-31 AMBIENTE E NUTRIZIONE S.r.l. Procédé pour la production de polyesters par synthèse catalysée par enzyme
WO2014202716A1 (fr) 2013-06-21 2014-12-24 Dupont Nutrition Biosciences Aps Procédés et compositions pour améliorer la valeur nutritive d'une biomasse lignocellulosique

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0231825A2 (fr) * 1986-02-04 1987-08-12 WALTER, Ulrich Procédé et installation de préparation de nourriture pour animaux ou de produits alimentaires
EP0710670A1 (fr) * 1994-11-03 1996-05-08 VOMM IMPIANTI E PROCESSI S.r.L. Procédé pour modifier l'amidon
US20040076715A1 (en) * 2002-10-16 2004-04-22 Mukund Parthasarathy Increased density pet food product and method of production
EP1847621A1 (fr) * 2006-04-21 2007-10-24 Vomm Impianti e Processi S.P.A. Procédé et installation destinés à la production de sucre fermentable à partir de matières contenant de la cellulose
EP2620462A1 (fr) * 2012-01-24 2013-07-31 AMBIENTE E NUTRIZIONE S.r.l. Procédé pour la production de polyesters par synthèse catalysée par enzyme
WO2014202716A1 (fr) 2013-06-21 2014-12-24 Dupont Nutrition Biosciences Aps Procédés et compositions pour améliorer la valeur nutritive d'une biomasse lignocellulosique

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110604487A (zh) * 2018-06-15 2019-12-24 广东美的生活电器制造有限公司 灭酶装置和食物料理机
EP3858969A1 (fr) * 2020-02-03 2021-08-04 Sekab E-Technology AB Agencement de prétraitement comprenant un dispositif de raclage
WO2021156245A1 (fr) * 2020-02-03 2021-08-12 Sekab E-Technology Ab Agencement de prétraitement comprenant un dispositif de raclage

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