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WO2016153355A3 - Novel isolation and amplification process control - Google Patents

Novel isolation and amplification process control Download PDF

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Publication number
WO2016153355A3
WO2016153355A3 PCT/NL2016/050216 NL2016050216W WO2016153355A3 WO 2016153355 A3 WO2016153355 A3 WO 2016153355A3 NL 2016050216 W NL2016050216 W NL 2016050216W WO 2016153355 A3 WO2016153355 A3 WO 2016153355A3
Authority
WO
WIPO (PCT)
Prior art keywords
microorganism
process control
nucleic acid
positive
amplification process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2016/050216
Other languages
French (fr)
Other versions
WO2016153355A2 (en
Inventor
Paul Hendrik Maria Savelkoul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microbiome Ltd
Original Assignee
Microbiome Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microbiome Ltd filed Critical Microbiome Ltd
Publication of WO2016153355A2 publication Critical patent/WO2016153355A2/en
Publication of WO2016153355A3 publication Critical patent/WO2016153355A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a positive control nucleic acid molecule comprising first and second primer hybridization sequences derived from a first microorganism which flank an internal nucleic acid sequence that is not naturally present in the first microorganism. The invention further relates to a positive process control microorganism comprising said positive control nucleic acid molecule, to a receptacle comprising said positive control microorganism, and to methods for determining whether a first microorganism is present or absent in a test sample, comprising contacting the test sample with the positive process control microorganism and generating amplification products using first and second primers that hybridize to the first and second primer hybridization sequences.
PCT/NL2016/050216 2015-03-26 2016-03-29 Novel isolation and amplification process control Ceased WO2016153355A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL2014529 2015-03-26
NL2014529 2015-03-26

Publications (2)

Publication Number Publication Date
WO2016153355A2 WO2016153355A2 (en) 2016-09-29
WO2016153355A3 true WO2016153355A3 (en) 2016-11-03

Family

ID=53502774

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2016/050216 Ceased WO2016153355A2 (en) 2015-03-26 2016-03-29 Novel isolation and amplification process control

Country Status (1)

Country Link
WO (1) WO2016153355A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070141563A1 (en) * 2005-12-21 2007-06-21 Roche Molecular Systems, Inc. Control for nucleic acid testing
WO2011150999A1 (en) * 2010-06-02 2011-12-08 Merck Patent Gmbh A genetically modified bacterium of the species listeria monocytogenes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
DE10021390C2 (en) 2000-05-03 2002-06-27 Juergen Olert Protection solution and fixation method for the paraffin section technique
US7138226B2 (en) 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
FR2852392B1 (en) 2003-03-12 2005-07-08 Inst Claudius Regaud TISSUE FIXATION COMPOSITION
GB0901001D0 (en) 2009-01-22 2009-03-04 Univ Nottingham Methods
EP2333105A1 (en) 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells
CN103518132B (en) 2011-06-06 2015-11-25 拜奥卡蒂斯股份有限公司 Selective lysis of cells by ionic surfactants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070141563A1 (en) * 2005-12-21 2007-06-21 Roche Molecular Systems, Inc. Control for nucleic acid testing
WO2011150999A1 (en) * 2010-06-02 2011-12-08 Merck Patent Gmbh A genetically modified bacterium of the species listeria monocytogenes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANA ABAD-DIAZ-DE-CERIO ET AL: "The aspHS gene as a new target for detecting Aspergillus fumigatus during infections by quantitative real-time PCR", MEDICAL MYCOLOGY, vol. 51, no. 5, 1 July 2013 (2013-07-01), Abingdon, pages 545 - 554, XP055228875, ISSN: 1369-3786, DOI: 10.3109/13693786.2012.756989 *
E. FLORES ET AL: "Real-time PCR with internal amplification control for detecting tuberculosis: method design and validation", APMIS, vol. 117, no. 8, 1 August 2009 (2009-08-01), DK, pages 592 - 597, XP055228750, ISSN: 0903-4641, DOI: 10.1111/j.1600-0463.2009.02508.x *
EVGENY V. USACHEV ET AL: "Internally controlled PCR system for detection of airborne microorganisms", JOURNAL OF ENVIRONMENTAL MONITORING, vol. 14, no. 6, 1 January 2012 (2012-01-01), GB, pages 1631, XP055228879, ISSN: 1464-0325, DOI: 10.1039/c2em30019b *
VAN DOORN R ET AL: "Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel internal amplification control approach using Biotrove OpenArrays.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY NOV 2009, vol. 75, no. 22, November 2009 (2009-11-01), pages 7253 - 7260, XP055228763, ISSN: 1098-5336 *

Also Published As

Publication number Publication date
WO2016153355A2 (en) 2016-09-29

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