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WO2016145925A1 - Assemblage de nanoparticules métalliques fortement résistant aux sels et son procédé de fabrication - Google Patents

Assemblage de nanoparticules métalliques fortement résistant aux sels et son procédé de fabrication Download PDF

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Publication number
WO2016145925A1
WO2016145925A1 PCT/CN2015/100036 CN2015100036W WO2016145925A1 WO 2016145925 A1 WO2016145925 A1 WO 2016145925A1 CN 2015100036 W CN2015100036 W CN 2015100036W WO 2016145925 A1 WO2016145925 A1 WO 2016145925A1
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WO
WIPO (PCT)
Prior art keywords
product
metal nanoparticle
salt
high salt
mixture
Prior art date
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Ceased
Application number
PCT/CN2015/100036
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English (en)
Chinese (zh)
Inventor
胡建强
余贵萍
李敏
陈宇宇
邓修龙
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South China University of Technology SCUT
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South China University of Technology SCUT
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Application filed by South China University of Technology SCUT filed Critical South China University of Technology SCUT
Publication of WO2016145925A1 publication Critical patent/WO2016145925A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B22—CASTING; POWDER METALLURGY
    • B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
    • B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Definitions

  • the invention belongs to the technical field of nano materials, and particularly relates to a high salt tolerance metal nanoparticle assembly and a preparation method thereof.
  • Metal nanoparticle assemblies have found wide applications in nanodevices, nanosensors, and nanomedicine.
  • Metal nano-assemblies not only have excellent optical, electrical, catalytic and other properties of isolated nanoparticles, but also have some new properties, such as surface-enhanced Raman properties, due to assembly, which greatly broadens the field of nano-particles in nanotechnology.
  • the scope of application within. With the increasing application of metal nanoparticle assemblies, especially in biomedicine, the salt tolerance is very high.
  • Metal nanoparticles, including metal nanoparticle assemblies are colloids that are particularly sensitive to electrolyte solutions such as salt solutions.
  • the primary object of the present invention is to provide a method for preparing a metal nanoparticle assembly having high salt tolerance, which is simple and efficient, has uniform product morphology, and good dispersibility.
  • Another object of the present invention is to provide a metal nanoparticle assembly obtained by the above production method, and to carry out an experiment for salt tolerance of the assembly.
  • a method for preparing a high salt tolerance metal nanoparticle assembly comprising the following steps:
  • the metal nanoparticle a according to the step (1) is Au, Ag or Cu.
  • the coating agent in the step (1) is sodium dodecyl sulfate (SDS); the coating agent and the metal nanoparticles are added
  • SDS sodium dodecyl sulfate
  • the mass ratio of a to ssDNA mixture a is 0.01% ⁇ 1%
  • PBS phosphate buffered saline
  • the buffer solution has a concentration of 10 to 100 mmol/L in a mixture a of metal nanoparticles a and ssDNA; the salt is added with metal nanoparticles a and
  • the concentration in the mixture of ssDNA a gradually increased from 6 to 24 h to the final 50 to 500 mmol/L.
  • the mixing culture time described in the step (1) is 6 to 24 h.
  • the separation and purification method is centrifugal separation or dialysis.
  • the metal nanoparticles b described in the step (2) and the metal nanoparticles in the step (1) are one or two of Au, Ag or Cu; the metal nanoparticles b have a size of 1 to 100 nm; the metal nanoparticles b
  • the molar ratio to sscDNA is 1:0.2 ⁇ 20.
  • the encapsulating agent in the step (2) is SDS, and the wrapping agent is added with metal nanoparticles b and
  • the mass ratio of the mixture b of sscDNA is 0.01% ⁇ 1%
  • the concentration of the mixture of sscDNA b is 10 ⁇ 100 mmol/L
  • the concentration of the salt in the mixture b of metal nanoparticles b and sscDNA is 50-500. Mmmol/L ;
  • the mixing culture time described in the step (2) is 6 to 24 h.
  • the separation and purification method is centrifugal separation or dialysis.
  • the concentration of the buffer solution in the mixed solution in which the product a and the product b are mixed is 10 to 100 mmol/L; the concentration of the salt in the mixed solution in which the product a and the product b are mixed is 50 ⁇ 500 mmol/L;
  • the mixed culture time is 6 ⁇ 24 h; the centrifugal speed is 5000 ⁇ 20,000 rpm; the centrifugation time is 5 ⁇ 30 min, centrifugation 1 ⁇ 3 times.
  • Salt tolerance test of the above metal nanoparticle assembly including stability in physiological saline and sodium chloride at different concentrations ( NaCl Stability in solution.
  • the method is easy to operate, has strong controllability and high assembly efficiency, and the obtained product has uniform structure and good dispersibility, and the product has strong salt tolerance and can withstand a salt concentration up to three times the concentration of physiological saline. Therefore, the metal nanoassemblies obtained by the preparation method have potential advantages in biological applications.
  • Figure 1 is a transmission electron micrograph (TEM) of the gold nanoparticle assembly obtained in Example 2;
  • Figure 2 is a transmission electron micrograph of the gold nanoparticle assembly obtained in Example 2 after incubation for 12 h in physiological saline ( TEM );
  • Figure 3 is a gold nanoparticle assembly obtained in Example 2 at different concentrations of NaCl
  • the salt tolerance test result in the solution, that is, the ultraviolet absorption spectrum.
  • the metal nanoparticles used in the method of the present invention are commercially available or can be prepared by themselves using the prior art.
  • the ssDNA and sscDNA used in the following examples were purchased from Shanghai Shenggong Bioengineering Co., Ltd.
  • ssDNA 5' -HS-(CH 2 ) 6 ATC CTG ACA TCG GCA CGA GTA TTT CTA CCA TGT ATC-3'
  • sscDNA 5'-HS-(CH 2 ) 6 GAT ACA TGG TAG AAA TAC TCGTGC CGA TGT CAG GAT-3'.
  • steps (1) and (2) are mixed at a molar ratio of 1:40 and added to a final concentration of 0.2.
  • the obtained gold nanoparticle assembly was subjected to TEM characterization, and the results are shown in Fig. 1.
  • the gold nanoparticles were successfully connected to the surface of 18 nm gold nanoparticles to form a 'nuclear-satellite' assembly.
  • the average number of satellites was nine, and the assembly was well dispersed and uniform in size.
  • steps (1) and (2) are mixed at a molar ratio of 1:80 and added to a final concentration of 0.2.
  • Example 2 The gold nanoparticle assembly of Example 2 was cultured in physiological saline for 12 hours, and then subjected to transmission electron microscopy (TEM). ) characterization. Its TEM image is shown in Figure 2.
  • TEM transmission electron microscopy
  • the gold nanoparticle assembly prepared by the method of the present invention is cultured in physiological saline for up to 12 h. It still retains its intact morphology, indicating its good stability at 0.9% salt concentration.
  • Example 2 The gold nanoparticle assembly of Example 2 was taken at 0.9%, 1.8%, 3.0%, 3.3, respectively. Salt tolerance tests were performed in % and 4.5% (mass fraction) NaCl solutions, and the initial color change of the solution was observed and UV characterized (results shown in Figure 3).
  • the stability of the gold nanoparticle assembly is easily affected by the salt concentration.
  • a normally uniformly dispersed aqueous solution of the assembly is generally ruby red, and agglomeration may occur when it is in a high concentration of salt solution. This is reflected in the appearance of the color change of the solution (usually purple), which is reflected in the ultraviolet spectrum as the red shift of the maximum absorption peak. Therefore, the stability of the solution can be verified by the color of the solution and the ultraviolet absorption spectrum.

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Nanotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Plant Pathology (AREA)
  • Composite Materials (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Manufacturing & Machinery (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Powder Metallurgy (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Manufacture Of Metal Powder And Suspensions Thereof (AREA)

Abstract

L'invention concerne un procédé de fabrication d'un assemblage de nanoparticules métalliques fortement résistant aux sels, ledit procédé consistant à : obtenir une nanoparticule métallique (a) et l'ADNss, ajouter ces derniers à un mélange (a) d'un agent de revêtement, d'une solution tampon et d'un sel, et séparer et purifier ces derniers après mélange et mise en culture, ce qui permet d'obtenir un produit (a) ; obtenir une nanoparticule métallique (b) et l'ADNssc, ajouter ces derniers à un mélange (b) de l'agent de revêtement, de la solution tampon et du sel, et séparer et purifier ces derniers après mélange et mise en culture, ce qui permet d'obtenir un produit (b) ; et mélanger le produit (a) et le produit (b), dissoudre ces derniers dans une solution mixte constituée de l'agent de revêtement, de la solution tampon et du sel, et centrifuger et laver ces derniers après agitation et mise en culture de ces derniers, ce qui permet d'obtenir l'assemblage de nanoparticules métalliques fortement résistant aux sels. L'invention concerne un assemblage de nanoparticules métalliques fortement résistant aux sels qui est fabriqué par le procédé ci-dessus. Le procédé est simple, très efficace et présente une contrôlabilité élevée. Le produit résultant présente un aspect uniforme, une bonne dispersibilité et une résistance élevée aux sels.
PCT/CN2015/100036 2015-03-18 2015-12-31 Assemblage de nanoparticules métalliques fortement résistant aux sels et son procédé de fabrication Ceased WO2016145925A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510118750.3 2015-03-18
CN201510118750.3A CN104923777A (zh) 2015-03-18 2015-03-18 一种高耐盐性金属纳米粒子组装体及其制备方法

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Cited By (1)

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CN117123794A (zh) * 2023-08-23 2023-11-28 哈尔滨工业大学(深圳)(哈尔滨工业大学深圳科技创新研究院) 一种表面具有纳米级棒状结构的微米银颗粒及其制备方法

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CN104923777A (zh) * 2015-03-18 2015-09-23 华南理工大学 一种高耐盐性金属纳米粒子组装体及其制备方法

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WO2006024023A2 (fr) * 2004-08-24 2006-03-02 Nanomix, Inc. Dispositifs de detection a nanotubes, destines a la detection de sequences d'adn
CN101541961A (zh) * 2006-10-04 2009-09-23 布鲁克哈文科学协会 Dna指导的纳米颗粒组装体
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Publication number Priority date Publication date Assignee Title
CN117123794A (zh) * 2023-08-23 2023-11-28 哈尔滨工业大学(深圳)(哈尔滨工业大学深圳科技创新研究院) 一种表面具有纳米级棒状结构的微米银颗粒及其制备方法

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