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WO2015138977A3 - Metabolic engineering of clostridium for biofuel production - Google Patents

Metabolic engineering of clostridium for biofuel production Download PDF

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Publication number
WO2015138977A3
WO2015138977A3 PCT/US2015/020568 US2015020568W WO2015138977A3 WO 2015138977 A3 WO2015138977 A3 WO 2015138977A3 US 2015020568 W US2015020568 W US 2015020568W WO 2015138977 A3 WO2015138977 A3 WO 2015138977A3
Authority
WO
WIPO (PCT)
Prior art keywords
disclosed
clostridium
biofuel production
metabolic engineering
clostridium bacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2015/020568
Other languages
French (fr)
Other versions
WO2015138977A2 (en
Inventor
Shang-Tian Yang
Xiaorui YANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ohio State Innovation Foundation
Original Assignee
Ohio State Innovation Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ohio State Innovation Foundation filed Critical Ohio State Innovation Foundation
Publication of WO2015138977A2 publication Critical patent/WO2015138977A2/en
Publication of WO2015138977A3 publication Critical patent/WO2015138977A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Disclosed herein is a cellulolytic acidogenic Clostridium bacterium transformed to overexpress aldehyde/alcohol dehydrogenase for biofuel production. Also disclosed are methods of expressing a heterologous gene sequence in a cellulolytic acidogenic Clostridium bacterium. Also disclosed herein are methods of producing a biofuel from cellulose. Disclosed is also an in vivo method for methylating a plasmid containing heterologous genes for expression in a Clostridium bacterium.
PCT/US2015/020568 2014-03-14 2015-03-13 Metabolic engineering of clostridium for biofuel production Ceased WO2015138977A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461953148P 2014-03-14 2014-03-14
US61/953,148 2014-03-14

Publications (2)

Publication Number Publication Date
WO2015138977A2 WO2015138977A2 (en) 2015-09-17
WO2015138977A3 true WO2015138977A3 (en) 2015-11-26

Family

ID=54072603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/020568 Ceased WO2015138977A2 (en) 2014-03-14 2015-03-13 Metabolic engineering of clostridium for biofuel production

Country Status (1)

Country Link
WO (1) WO2015138977A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3775245A1 (en) * 2018-03-28 2021-02-17 SilicoLife Lda. Method for n-butanol production using heterologous expression of anaerobic pathways
CN119592595B (en) * 2024-12-04 2025-08-08 杭州师范大学 A method for constructing giant organelles in Escherichia coli to enhance the oxygen tolerance of [Fe-Fe] hydrogenase
CN119776393A (en) * 2024-12-16 2025-04-08 东北大学 A novel and efficient genetic transformation method for Clostridium pasteurianum

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110236941A1 (en) * 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
US20120190090A1 (en) * 2011-01-26 2012-07-26 The Regents Of The University Of California Microbial conversion of plant biomass to advanced biofuels
US20120196338A1 (en) * 2008-07-28 2012-08-02 Qteros, Inc. Methods and compositions for improving the production of products in microorganisms
US20130323809A1 (en) * 2010-10-01 2013-12-05 The Ohio State University Metabolic engineering of clostridium tyrobutyricum for butanol production

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120196338A1 (en) * 2008-07-28 2012-08-02 Qteros, Inc. Methods and compositions for improving the production of products in microorganisms
US20130323809A1 (en) * 2010-10-01 2013-12-05 The Ohio State University Metabolic engineering of clostridium tyrobutyricum for butanol production
US20110236941A1 (en) * 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
US20120190090A1 (en) * 2011-01-26 2012-07-26 The Regents Of The University Of California Microbial conversion of plant biomass to advanced biofuels

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DU, Y.: "High-Yield And High-Titer n-Butanol Production From Lignocellulosic Feedstocks By Metabolically Engineered Clostridium Tyrobutyricum.", DISSERTATION., 2013 *

Also Published As

Publication number Publication date
WO2015138977A2 (en) 2015-09-17

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