WO2015125997A1 - Method for detecting multi-layered structure of liquid crystal emulsion by measuring mueller matrix - Google Patents

Abstract

The present invention relates to a test method capable of detecting a multi-layered structure of a liquid crystal emulsion by using a phase difference of light passing through an anisotropic material. A method for detecting a multi-layered structure, according to the present invention, reduces damage to samples, which can occur during a pretreatment process, compared with that of a detection method using a conventional electron microscope and can more easily and quickly detect the multi-layered structure of the liquid crystal emulsion. In addition, the method for testing the multi-layered structure of the liquid crystal emulsion, according to the present invention, can detect the multi-layered structure of the liquid crystal emulsion, which cannot be recognized by a test method of a conventional polarizing microscope, and information on an internal structure thereof, and thus can be utilized as a useful quality evaluation tool during the preparation of the liquid crystal emulsion.

Description

 [Specifications

[Name of invention]

 Method of confirming multi-layered structure of liquid crystal emulsion by back matrix measurement

Technical Field

 The present invention relates to a test method for confirming the multilayered structure of a liquid crystal emulsion using a Muller matrix measurement for measuring the polarization state of incident light and transmitted light to an anisotropic material.

Background Art

The stratum corneum protects the skin from external harmful substances and performs a skin barrier function that protects the thorax by inhibiting water vaporization in the human body. The core of the skin barrier function of the stratum corneum is the lamellae (layered) structure composed of the intercellular lipid components of the stratum corneum. In the cosmetics industry, liquid crystal emulsion formulations have been developed by simulating the lamellar structure of these intercellular lipid components (Suzuki et al., J. Soc. Cosmet. Chem. Jpn. 27 (3) 193-205 1993). However, the liquid crystal emulsion has a problem in that when the lamellar structure is not formed densely, moisture penetrates between the structure's name and the name, and the particles themselves swell. In particular, it has been reported that liquid crystal emulsions composed of POE-based surfactants may cause such swelling in the emulsion system and ultimately affect the stability of the entire emulsion system (GM Eccleston, Multiple-phase oil—in—water emulsions, J. Soc.Cosmet.Chem., (41) 1-22 1990). Therefore, observing how well the lamellar structure is formed in preparing the liquid crystal emulsion is a very important factor in terms of quality. Conventionally, a polarizing microscope and an electron microscope were used to confirm the lamellar structure of the liquid emulsion. In the case of a polarizing microscope, it is an equipment for observing the anisotropy degree by the lamellar structure of the liquid crystal emulsion. However, it is a disadvantage to not know the lamellar structure directly, but to observe the Maltese cross, which is a characteristic image of the liquid crystal emulsion due to the polarization of the liquid crystal emulsion. In addition, in the case of electron microscope There is an advantage in that accurate information about the lamellar structure and particle shape of the liquid crystal emulsion can be confirmed, but problems of equipment use and complicated and difficult pretreatment were disadvantages. In addition, it is not easy to obtain visual information on the lamellar structure of the liquid crystal emulsion because there is a possibility of sample damage, such as the lamellar structure may be deformed during the lyophilization process to observe the lamellar structure.

While investigating how to easily observe the lamellar structure of liquid crystal emulsions without any pretreatment, the researchers obtained an idea from measurement equipment for anisotropic features used in the liquid crystal display (LCD) industry, which is a heterogeneous industry. It was written. LCDs generally consist of two glass panels, with a thin layer of liquid crystal sealed between them. When one of these glass panels receives a voltage and the temperature rises, an electric field is applied to adjacent liquid crystal molecules of the liquid crystal material. When applied, the orientation of the molecules changes, resulting in a display phenomenon. The liquid crystal used in the LCD industry is a thermotropic liquid crystal driven by temperature and is distinguished from a lipophilic liquid crystal driven by concentration. Since the cosmetic industry uses only a lyophilic liquid crystal, it is the same liquid crystal phase, but the method of measuring it varies depending on the industrial field. LCDs are based on an array of small transparent electrodes that form pixels, each pixel having electrically adjustable light transmission characteristics that are adjusted by varying the voltage level applied to the electrodes. In this case, the design technology of the LCD is important, and there are various parameters such as a rubbing direction in which a molecular axis of liquid crystal molecules is directed, and a control of twist angle by appropriately selecting a rubbing direction of a second glass surface. In addition, the technique of measuring the thickness of the liquid crystal cell (US Patent No. 5,239,365) is also one of the important parameters of the LCD technology. In addition, according to US Patent No. 8,325,340, it is possible to measure the cell gap, twist angle, rubbing direction, etc. of the liquid crystal cells by full muller matrix measurement. This technique is a method of measuring the optical characteristics of the LCD cell is completely different from the anisotropic measurement field of the liquid crystal emulsion, which is a liquid-liquid liquid crystal conventionally used in the cosmetic industry. However, the lyophilic liquid crystal emulsion has an anisotropic characteristic because it has a multilayered structure. When this anisotropic characteristic is obtained, the difference in the speed of light passing through the long axis and short axis is generated, and the phase difference is measured. The researchers focus on these anisotropic features of lyophilic liquid crystalline emulsions. It was thought that the multilayered structure of the regular emulsion could be observed.

 Accordingly, the present inventors have made diligent research efforts to overcome the problems of the prior arts. As a result, using the Mueller matrix measurement for measuring the polarization states of incident and transmitted light, not only the multilayer structure of the liquid crystal emulsion but also the interior of the particles It was confirmed that the information about can be easily understood, and completed the present invention.

[Details of the Invention]

 [Technical problem]

 Therefore, the main object of the present invention is to provide a test method to check the lamellar structure of the liquid crystal emulsion through a matrix method and to obtain information on the internal structure of the liquid crystal emulsion. Another object of the present invention is to evaluate the quality of the liquid crystal emulsion prepared by using the test method for the lamellar structure of the liquid crystal emulsion.

Technical Solution

 In order to achieve the above object, the present invention provides a method for confirming the multi-layer structure of the liquid crystal emulsion using Mueller Matrix Polarimetry for measuring the polarization state of the incident light and transmitted light to the anisotropic material.

 In the present invention, there is provided a method for identifying a multi-layered structure of a liquid crystal emulsion, preferably comprising the following steps:

 a) preparing a sample comprising a liquid crystal emulsion dog;

 b) placing the sample on a sample stage of a Mueller Matrix Polarimeter;

 c) injecting light in a polarized state into the sample;

 d) detecting light in a polarization state transmitted from the sample;

 e) calculating a muller matrix from the detected polarized light; f) converting the calculated Muller matrix into an image; And ，

 g) identifying the multilayered structure of the liquid crystal emulsion from the image.

In the present invention, preferably, in the step a), there is no pretreatment process such as lyophilization of the sample. Provide the law. In the case of the conventional electron microscope, there was a possibility of sample damage, such as the lamellar structure may be deformed during the pretreatment process such as lyophilization to observe the lamellar structure of the liquid crystal emulsion, but in the case of the method of the present invention, the prepared liquid crystal emulsion sample Since it can be used as is, there is no possibility of sample damage.

 In the present invention, preferably, in step b), the sample containing the liquid crystal emulsion is coated on the slide glass, and the cover glass is covered thereon, and then spread evenly by applying an appropriate pressure. It provides a method for confirming the multi-layered structure of the liquid crystal emulsion, characterized in that it is placed on the stage. In the above, the appropriate pressure is a degree in which the liquid sample is uniformly spread on the slide glass and the cover glass and can be easily adjusted by hand.

 In the present invention, preferably, in step b), the Muller matrix measuring instrument provides a method for confirming the multi-layer structure of the merger in the liquid crystal, which uses the same thing as the LCD optical characteristic measuring apparatus. The Muller matrix measuring instrument that can be used in the present invention can be used any device that can analyze the polarization state of the sample that was conventionally mainly used for measuring the LCD optical characteristics. In the embodiment of the present invention was used AXO STEP (AXOMETRICS Co., Ltd.), a Mueller matrix measuring instrument used in the LCD industry.

 In the present invention, preferably, the incident light in the step c) provides a method for identifying a multi-layered structure of the liquid crystal emulsion, characterized in that the light generated from the light source to have various polarization state through the polarization generator. The light source may be a luminable visible light source, a short wavelength LED or a laser, but is preferably a laser. The polarization generator includes a polarizer and a controller such as angle and type of polarization. In the present invention, in order to observe the variation of various polarization states by the sample, the polarization generator may induce various polarization states of different directions (angles), ellipticity, and helix in the incident light.

In the present invention, preferably, in step d), the transmitted light provides a method for confirming the multi-layer structure of the liquid crystal emulsion, characterized in that the variation of the polarization state is caused by the sample. In the present invention, the variation of linear, circular, or elliptical retardance or diattenuation is induced by the sample. The Mueller matrix is calculated through the variation of the polarization state.

 In the present invention, preferably the detection in step d) provides a method of confirming the multi-layer structure of the liquid crystal emulsion characterized in that it uses a CCD detector. The CCD converts an optical signal transmitted from a sample into an electrical signal and converts the electrical signal into an image through a computer processor for display. In the present invention, preferably, in the step e), the back matrix is a 4x4 full back matrix provides a method of confirming the multi-layer structure of the liquid crystal emulsion. The muller matrix of the present invention may be a partial back matrix of less than 4 × 4, but is preferably characterized as a 4 × 4 full muller matrix. The full muller matrix can be achieved through the generation and detection of various polarization states.

 In the present invention, preferably, the image conversion in the step f) is to form a spatially resolved matrix over the entire area of the sample, and to convert the spatially resolved matrix into a spatially resolved image Provided is a method for identifying a multilayered structure of an emulsion. Such image transformation of the back matrix is possible using any conventional Mueller matrix measuring instrument equipped with a display, for example, the disclosed imaging apparatus of the prior art document (US2004012853A1, Method and apparatus for imaging using polarimetry and matrix based image reconstruction). Alcohol can also be used.

 In the present invention, preferably, in the step f) it provides a method for identifying a multi-layered structure of the liquid crystal emulsion, characterized in that the conversion of the Muller matrix to a 2D or 3D image. In the present invention, the multi-layered structure of the liquid crystal emulsion can be confirmed in more detail by converting the 2D image due to the color difference into the 3D image.

In the present invention, preferably in the step g) the core of the liquid crystal emulsion is shown as a blue region because there is no anisotropy, the circular interface of the liquid crystal emulsion has a red phase (the phase difference of light is generated by the multi-lamellar structure) Provided is a method for confirming the multi-layered structure of a liquid crystal emulsion, which is represented by red). The color may be set differently in the order of red nochopa Nambo according to the magnitude of the phase difference (ie, retardance). In the present invention, in the case of liquid crystal emulsions, anisotropy is generated due to the birefringence property due to the multilamellar structure, thereby generating a specific image as shown in FIG. 3. In liquid crystal, there is no anisotropy in the inner core, so it appears as a blue area, and the phase difference of light is generated by the multi-lamellar structure present in the interface region of the water phase and the oil phase, and it is digitally converted into a red series. In addition, various colors appearing at the circular interface of the liquid crystal emulsion particles may be interpreted as phase differences of light generated differently for each layer due to the multi-layered structure.

 In the present invention, preferably, the step g) provides a method for identifying a multi-layered structure of a liquid crystal emulsion, characterized in that not only the multi-layered structure of the liquid crystal emulsion but also information on the internal structure can be confirmed. Since the method of the present invention can check not only the multilayer structure of the liquid crystal emulsion but also the internal structure of the liquid crystal emulsion, which can not be seen in the conventional polarization microscope test method, it can be used as a quality evaluation tool useful in preparing the liquid crystal emulsion. Hereinafter, the present invention will be described in detail.

 The Mueller matrix is a matrix expression for dealing with Stokes vectors describing the polarization states of light. This method was devised by Hans Muller, professor of physics at the Massachusetts Institute of Technology in 1943. Any fully polarized, partially polarized, or unpolarized light can be represented by Stokes vector (S), and any optical element can be represented by a Muller matrix (M). If the state before light passes through an optical element is S and the state after light is S ', it can be described as follows.

 S '= MS

상기 식에서, SO, SI, S2, 및 S3은 스토크스 백터 요소들이고， PH는 수평 선형 편광기 (horizontal linear polarizer, 0°), PV는 수직 선형 편광기 (vertical linear polarizer, 90°), P45는 45° 선형 편광기 (linear polarizer), P135는 135° 선형 (linear polarizer), PR는 우선 원형 편광기 (right circular polarizer), PL는 좌선 원 형 편광기 (left circular polarizer)로 실시된 플럭스 (flus) 측정값이다. 스토크스 백 터는 반드시 상기 6개 이상적인 편광기 (polarizer)로 측정될 필요는 없으며， 다른 플럭스 측정값도 사용될 수 있다. That is, Stokes vector S is used to describe the polarization state of light, and Muller matrix M is used to represent the polarization change characteristic of the optical element (sample). For example, Stokes vector S can be represented by the following equation (Shurcliff, WA, Polarized Light, Harvard University Press, Cambridge, Mass., 1962).

Where SO, SI, S2, and S3 are Stokes vector elements, PH is a horizontal linear polarizer (0 °), PV is a vertical linear polarizer (90 °), and P45 is 45 ° A linear polarizer, P135 is a 135 ° linear polarizer, PR is a right circular polarizer, and PL is a left circular polarizer. Stokes vectors are not necessarily measured with the six ideal polarizers, but other flux measurements may be used.

 The Mueller matrix M of the sample for changing the polarization state can be expressed by the following equation (2) as a matrix (matrix) for converting the Stokes vector S of the incident light into the Stokes vector S 'of the emitted (reflected or transmitted) light.

상기와 같은 풀 (full) 뮬러 매트릭스는 16개의 요소들을 갖는 4x4 행렬으로 서， 그 요소들안에 선형 (linear), 원형 (circular), 또는 타원형 (elliptical)의 디어테뉴 에이션 (diattenuation), 지연 (retardance), 및 탈분극 (depolarization)의 모든 편광 특 성을 내포하고 있기 때문에 편광상태를 특성화하기에 적합하다 구체적으로， 본 발명의 실시예에 있어서， 첫 번째 단계는 라멜라 구조를 갖지 아니하는 일반에멀견과 라멜라 구조를 갖는 액정에멀젼을 제작하는 것이다ᅳ 기준시료의 제조는 다음과 같다. 유상과 수상원료를 각각 평량한 후 72— 75°C로 가온한다. 오일파트를 물 파트에 투입하고， 유화과정을 거친 다음， 30^°C로 냉각한 이후， 탈기를 하여 완성한다. 두 번째 단계는 상기와 같이 만들어진 일반에멀젼과 액정에멀젼을 슬라이 드 글라스 위에 도포하고, 그 위에 커버글라스를 덮은 다음， 적당한 압력을 가하 여 균일하게 퍼지게 한다. 슬라이드 글라스와 커버 글라스 사이에 존재하는 에멀 젼의 양이 너무 적거나 많을 경우에는 원하는 이미지를 측정하기 어렵기 때문에 적당한 양의 에멀젼을 도포하는 것이 중요하다.

Such a full Mueller matrix is a 4x4 matrix with 16 elements, with linear, circular, or elliptical diattenuation, retardance within the elements. ) And all polarization properties of depolarization are suitable for characterizing the polarization state. Specifically, in the embodiment of the present invention, the first step is a general emulsion with no lamellar structure. The liquid crystal emulsion having a lamellar structure is prepared. The preparation of the reference sample is as follows. Weigh the oil and water materials separately and warm them to 72-75 ° C. The oil part is added to the water part, emulsified, cooled to 30 ^° C, and then degassed. The second step is to apply the general emulsion and liquid crystal emulsion prepared as above on the slide glass, cover the cover glass, and then apply an appropriate pressure to spread evenly. If the amount of emulsion present between the slide glass and the cover glass is too small or too large, it is important to apply the appropriate amount of emulsion because it is difficult to measure the desired image.

 The final step is to take the sample made above and measure it on the matrix instrument. The Muller Matrix equipment calculates the phase difference of the light passing through the emulsion particles, and then converts the figure to determine whether the lamellar structure of the liquid crystal emulsion is formed and the overall structure of the liquid crystal emulsion. To date, three evaluation methods for the multilamellar structure of liquid crystal emulsions have been known.

 1) Method of measuring constant distance between layers of lamellar structure using Small Angle X-ray Diffraction (SAXD) equipment using X-ray diffraction (graph data, not image)

 2) Method of observing Maltese cross pattern, which is an optical characteristic, for polarization of liquid crystal emulsion multi-lamellar structure using polarization microscope (image data)

 3) Method of directly checking the multi-lamellar structure by cutting the particles using an electron microscope and observing the cross section directly (image data)

 Among these methods, the first and third methods had to use expensive equipment, and the pretreatment was complicated and difficult. Especially, the third method had the possibility of sample deformation during the pretreatment. In addition, although method 2 is easy to pretreat and has a simple meas- urement, the Maltese cross pattern only confirms the anisotropy of the liquid crystal, and detailed information on the particle structure and the multi-lamellar structure is not known.

In order to solve the problem of the existing method, the present invention has developed a method for evaluating the structure of the liquid crystal emulsion multi-lamellae, which can see the advantages of 1-3. Advantages of the evaluation method of the present invention are 1) easy to pretreatment, 2) the direction and structure of the long axis and short axis of the lamellar layer forming the liquid crystal can be directly confirmed by the image, 3) the multi-lamellar structure of the liquid crystal emulsion particles as in the electron microscope image It is possible to observe directly. In the present invention, as a result of analyzing the Muller matrix image and the conventional electron microscope image of the liquid crystal emulsion, it was confirmed that there is a circular space therein and a multi-layered structure at the interface. Since general emulsion is optically round, Mueller Matrix Polarimetry observation shows no image, but liquid crystal emulsion has anisotropy due to birefringence due to multi-lamellar structure, resulting in a specific image as shown in FIG. The liquid crystal emulsion appears in the blue region because there is no anisotropy in the inner core, and the phase difference of light is generated by the multi-lamellar structure present in the interface region of the water phase and the oil phase, and it is digitally converted into a red series. These images showed the same images as those measured with an electron microscope. Therefore, the back matrix method of the present invention may be a new evaluation method that can easily check the multi-layer structure of the liquid crystal emulsion.

Advantageous Effects

 Lamellar structure evaluation method of the liquid crystal emulsion according to the present invention is characterized by knowing the information on the lamellar structure of the liquid crystal emulsion and the overall structure of the particles with a simple pretreatment. This test method can be easily applied as an evaluation method for lamellar structure in mass production of liquid crystal emulsion, and thus, it is easy to evaluate the quality of liquid crystal emulsion.

[Brief Description of Drawings]

 1 is an image of a polarizing microscope of a liquid crystal emulsion made according to an embodiment of the present invention.

⁴ is a Mueller matrix image for a general emulsion and a liquid crystal emulsion made according to an embodiment of the present invention.

 3 is a partially enlarged photograph of an electron microscope and a Mueller matrix image of liquid crystal emulsion particles according to an embodiment of the present invention.

4 is a photograph quantitatively and qualitatively analyzing anisotropic characteristics due to the multi-layered structure of a liquid crystal emulsion prepared in accordance with an embodiment of the present invention and a commercially available liquid crystal emulsion. 5 schematically illustrates an example of a Muller matrix meter that can be used in the present invention. A diode laser is used as the light source, and the polarization state of the incident light can be controlled by a polarization state generator (PSG) including a linear polarizer (PI) and a quarter-wave plate (QWP1). The polarization state of the light transmitted through the sample (S) is selected by a polarization state analyzer (PSA) including a linear analyzer (P2) and a quarter-wave plate (QWP2). It is detected by a CCD camera.

[Mode for carrying out the invention]

 Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only intended to illustrate the present invention, and the scope of the present invention is not to be construed as limited by these examples.

Example 1

In order to prepare a general emulsion and a liquid crystal emulsion used in this test method, an emulsion was prepared in the composition shown in Table 1 below.

Table 1

<Production method>

 1) In Table 1, the raw materials 1 to 13 were mixed and dissolved at 70-80 ° C. to prepare an oily solution.

 2) The raw materials 14-17 of Table 1 were mixed in a separate container, and warmed and dissolved to 70-80 ° C. to prepare an aqueous solution.

3) The oily solution of 1) was added to the aqueous solution of 2) and stirred with a homo mixer. 4) The raw material 18 of Table 1 was mixed with the mixed solution of 3), and the raw material 19 was then mixed.

 5) As soon as the process of 4) is finished, it was cooled. Iogo, raw materials 20 and 21 of Table 1 were added, and then bubbles were removed. Comparative Example 1 formed a general 0 / W emulsion, and Example 1 formed a liquid crystal emulsion. In Comparative Example 1 and Example 1 prepared in Example 1 using a polarizing microscope to observe the formation of a liquid crystal emulsion. The microscope used was ympus BX 51 (Olympus, Japan), and a polarizing microscope image was obtained using a polarizing lens. In Comparative Example 1, no image was observed on the polarizing microscope. On the contrary, in Example 1, the polarization microscope observation result showed that the maltese cross, which is a specific image of the liquid crystal emulsion, was clearly shown (see FIG. 1).

Example 2

 The matrix image and the electron microscope image were checked with the general emulsion and the liquid crystal emulsion prepared in Example 1. A Muller matrix measuring instrument, AxoStep (Axometrics, USA) was used for the back matrix experiment. The emulsion prepared in Example 1 is applied onto the slide glass without pretreatment, covered with a cover glass, and then uniformly spread by applying an appropriate pressure. The prepared slide glass was placed in the sample stage of AxoStep, and the muller matrix was measured by incidence of various angles of incidence and polarized laser light, and the desired image was obtained on a computer monitor connected to AxoStep. Electron microscopic images were observed using FEI-Quanta FIB Gatan-alto2500. First, the liquid crystal emulsion sample was frozen using liquid nitrogen, placed in a chamber connected with an electron microscope, and the frozen liquid crystal emulsion was broken using a micro knife, and the cross section of the broken liquid crystal emulsion was observed through an electron microscope. .

As a result of confirming the Muller matrix image, as shown in FIG. 2, there was no characteristic image in Comparative Example 1 (general 0 / W emulsion), and in Example 1, there were many round particles showing various colors. Shown in the particles of Example 1 Note that the various colors are due to the conversion of the phase difference generated as light passes through the particles of Example 1 into an image, which shows that the particles of Example 1 are anisotropic materials with a multilayer lamellar structure. In FIG. 3, when these particles are enlarged, the inside of the particle is the same blue color as the surrounding space, indicating that it is an isotropic space in which the phase difference of light does not occur, and the particle interface is red due to the large phase difference of light due to the anisotropic characteristic. . In addition, various colors appearing at the interface of the liquid crystal emulsion particles can be interpreted as a phase difference of light generated differently in each layer due to the multi-layer structure, and it is understood that the multi-layer structure is formed through an orderly arrangement at the interface of the particles. Can be. Figure 3 also shows an electron microscope image of Example 1, which, like the result of the Muller matrix, shows that the internal space is empty and the multilayer structure is formed at the interface. That is, it can be seen that the structure of the liquid crystal emulsion obtained through the electron microscope image and the structure of the liquid crystal emulsion obtained through the back matrix image are the same structure.

Example 3

 The liquid crystal emulsion prepared in Example 1 and the commercially available liquid crystal emulsion of N Company were purchased, and the anisotropic characteristics resulting from the multilayered structure of the liquid crystal emulsion were quantitatively and qualitatively analyzed. The matrix image checking was performed in the same manner as in Example 2. As a result, as shown in Figure 4, the liquid crystal emulsion prepared according to Example 1 (left) has a large number of liquid crystal emulsion particles showing a clear phase difference as a whole, in the case of commercially available liquid crystal emulsion of N company (right) Does not show a distinct phase difference. Therefore, the muller matrix measuring method according to the present invention may be used as a quality evaluation tool useful in preparing a liquid crystal emulsion.

Claims

【Scope of Claim】 【Claim 1】 A method of confirming the multilayer structure of liquid crystal emulsion using Mueller Matrix Polarimetry, which measures the polarization state of incident and transmitted light for an anisotropic material. 【Claim 2】 The method of claim 1, comprising the following steps: a) preparing a sample containing a liquid crystal emulsion; b) placing the sample on the sample stage of the matrix meter; c) incident polarized light on the sample; d) detecting light in a polarized state transmitted from the sample; e) calculating a Muller matrix from the detected light in the polarization state; f) converting the calculated Muller matrix into an image; and, g) Confirming the multilayer structure of the liquid crystal emulsion from the image. 【Claim 3】 The method of claim 2, wherein in step a), there is no pretreatment process such as freeze-drying the sample. 【Claim 4】 The method of claim 2, wherein in step b), the sample containing the liquid crystal emulsion is applied on a glass slide, covered with a cover glass, and then spread evenly by applying appropriate pressure, and then placed on the sample stage of the Mueller matrix measuring device. A method for confirming the multilayer structure of a liquid crystal emulsion, characterized in that it is placed on a bed. [Claim 5] 3. The method of claim 2, wherein in step b), the Muller matrix measuring device is used to measure LCD optical characteristics. A method for confirming the multilayer structure of liquid crystal emulsion, characterized by using the same sex measuring device. 【Claim 6】 The method of claim 2, wherein in step c), the incident light is made to have various polarization states through a polarization generator to generate light from a light source. 【Claim 7】 The method of claim 2, wherein in step d), a change in polarization state of the transmitted light is caused by the sample. 【Claim 8】 The method of claim 2, wherein in step d), the detection is performed using a CCD detector. 【Claim 9】 The method of claim 2, wherein in step e), the Muller matrix is a 4x4 full Muller matrix. [Claim 10] The liquid crystal emulsion according to claim 2, wherein the image conversion in step f) constructs a spatially resolved matrix over the entire area of the sample, and converts the spatially resolved matrix into a spatially resolved image. Method for confirming multilayer structure. 【Claim 11】 The method according to claim 2, wherein in step f), the Mueller matrix is converted into a 2D or 3D image. 【Claim 12】 According to claim 2, in step g), the core of the liquid crystal emulsion appears as a blue area because there is no anisotropy, and the circular interface of the liquid crystal emulsion turns red due to a phase difference of light due to the multi-lamellar structure. A method for confirming the multilayer structure of a liquid crystal emulsion, characterized in that it appears. [Claim 13] The method of claim 2, wherein in step g), not only the multilayer structure of the liquid crystal emulsion but also information on the internal structure can be confirmed.