[go: up one dir, main page]

WO2015112896A3 - Méthodes et compositions pour des séquences guidant le ciblage de cas9 - Google Patents

Méthodes et compositions pour des séquences guidant le ciblage de cas9 Download PDF

Info

Publication number
WO2015112896A3
WO2015112896A3 PCT/US2015/012747 US2015012747W WO2015112896A3 WO 2015112896 A3 WO2015112896 A3 WO 2015112896A3 US 2015012747 W US2015012747 W US 2015012747W WO 2015112896 A3 WO2015112896 A3 WO 2015112896A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methods
cas9 targeting
sequences
guiding cas9
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2015/012747
Other languages
English (en)
Other versions
WO2015112896A2 (fr
WO2015112896A9 (fr
Inventor
Rodolphe Barrangou
Kurt M. SELLE
Alexandra E. BRINER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
North Carolina State University
Original Assignee
North Carolina State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by North Carolina State University filed Critical North Carolina State University
Priority to CA2936646A priority Critical patent/CA2936646C/fr
Priority to EP15740979.8A priority patent/EP3097212A4/fr
Priority to JP2016547162A priority patent/JP2017503514A/ja
Priority to US15/113,656 priority patent/US10787654B2/en
Publication of WO2015112896A2 publication Critical patent/WO2015112896A2/fr
Publication of WO2015112896A3 publication Critical patent/WO2015112896A3/fr
Publication of WO2015112896A9 publication Critical patent/WO2015112896A9/fr
Anticipated expiration legal-status Critical
Priority to US17/002,133 priority patent/US20210017507A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des méthodes et des compositions pour l'édition du génome et le ciblage de l'ADN de protéines.
PCT/US2015/012747 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9 Ceased WO2015112896A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2936646A CA2936646C (fr) 2014-01-24 2015-01-23 Methodes et compositions pour des sequences guidant le ciblage de cas9
EP15740979.8A EP3097212A4 (fr) 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9
JP2016547162A JP2017503514A (ja) 2014-01-24 2015-01-23 Ｃａｓ９ターゲッティングをガイドする配列に関する方法および組成物
US15/113,656 US10787654B2 (en) 2014-01-24 2015-01-23 Methods and compositions for sequence guiding Cas9 targeting
US17/002,133 US20210017507A1 (en) 2014-01-24 2020-08-25 Methods and compositions for sequences guiding cas9 targeting

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461931515P 2014-01-24 2014-01-24
US61/931,515 2014-01-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US15/113,656 A-371-Of-International US10787654B2 (en) 2014-01-24 2015-01-23 Methods and compositions for sequence guiding Cas9 targeting
US17/002,133 Division US20210017507A1 (en) 2014-01-24 2020-08-25 Methods and compositions for sequences guiding cas9 targeting

Publications (3)

Publication Number Publication Date
WO2015112896A2 WO2015112896A2 (fr) 2015-07-30
WO2015112896A3 true WO2015112896A3 (fr) 2015-10-29
WO2015112896A9 WO2015112896A9 (fr) 2015-11-26

Family

ID=53682132

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/012747 Ceased WO2015112896A2 (fr) 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9

Country Status (4)

Country Link
EP (1) EP3097212A4 (fr)
JP (3) JP2017503514A (fr)
CA (1) CA2936646C (fr)
WO (1) WO2015112896A2 (fr)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013024337A2 (pt) 2011-03-23 2017-09-26 Du Pont locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
WO2013066438A2 (fr) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Évaluation et amélioration de la spécificité de clivage des nucléases
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
EP3611268A1 (fr) 2013-08-22 2020-02-19 E. I. du Pont de Nemours and Company Modification du génome de plantes utilisant des systèmes d'endonucléase d'arn/cas de guidage et procédés d'utilisation
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
WO2015066119A1 (fr) 2013-10-30 2015-05-07 North Carolina State University Compositions et méthodes liées à un système crispr-cas de type ii chez lactobacillus buchneri
KR20230054509A (ko) 2013-11-07 2023-04-24 에디타스 메디신, 인코포레이티드 지배적인 gRNA를 이용하는 CRISPR-관련 방법 및 조성물
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
EP3097212A4 (fr) * 2014-01-24 2017-10-04 North Carolina State University Méthodes et compositions pour des séquences guidant le ciblage de cas9
CN106460003A (zh) 2014-04-08 2017-02-22 北卡罗来纳州立大学 用于使用crispr相关基因rna引导阻遏转录的方法和组合物
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016033298A1 (fr) 2014-08-28 2016-03-03 North Carolina State University Nouvelles protéines cas9 et éléments de guidage pour le ciblage de l'adn et l'édition du génome
WO2016040030A1 (fr) 2014-09-12 2016-03-17 E. I. Du Pont De Nemours And Company Production de sites d'intégration spécifique de site, pour des loci de traits complexes dans le maïs et le soja, et procédés d'utilisation
US10450576B2 (en) 2015-03-27 2019-10-22 E I Du Pont De Nemours And Company Soybean U6 small nuclear RNA gene promoters and their use in constitutive expression of small RNA genes in plants
EP3294877A1 (fr) * 2015-05-15 2018-03-21 Pioneer Hi-Bred International, Inc. Caractérisation rapide de systèmes d'endonucléases cas, séquences pam et éléments d'arn guide
EP3303607A4 (fr) 2015-05-29 2018-10-10 North Carolina State University Procédés pour le criblage de bactéries, d'archées, d'algues et de levure à l'aide d'acides nucléiques crispr
CN107922918B (zh) 2015-06-15 2022-10-21 北卡罗来纳州立大学 用于有效递送核酸和基于rna的抗微生物剂的方法和组合物
US9580727B1 (en) * 2015-08-07 2017-02-28 Caribou Biosciences, Inc. Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
WO2017058751A1 (fr) 2015-09-28 2017-04-06 North Carolina State University Méthodes et compositions pour agents antimicrobiens spécifiques d'une séquence
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
WO2017112620A1 (fr) 2015-12-22 2017-06-29 North Carolina State University Méthodes et compositions pour l'administration d'agents antimicrobiens à base de crispr
CN105594664B (zh) * 2016-02-16 2018-10-02 湖南师范大学 一种基因敲除选育stat1a基因缺失型斑马鱼的方法
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (fr) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Édition du gène récepteur ccr5 pour protéger contre l'infection par le vih
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
EP3592853A1 (fr) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression de la douleur par édition de gène
EP3592381A1 (fr) 2017-03-09 2020-01-15 President and Fellows of Harvard College Vaccin contre le cancer
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CA3057192A1 (fr) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Editeurs de nucleobase comprenant des proteines de liaison a l'adn programmable par acides nucleiques
WO2018209320A1 (fr) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Arn guides incorporés par aptazyme pour une utilisation avec crispr-cas9 dans l'édition du génome et l'activation transcriptionnelle
EP3630975B1 (fr) * 2017-05-26 2025-11-19 North Carolina State University Arn guides modifiés pour moduler l'activité cas9 et procédés d'utilisation
EP3652312A1 (fr) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systèmes et procédés d'intégration ciblée et d'édition du génome et détection de celle-ci à l'aide de sites d'amorçage intégrés
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (fr) 2017-08-30 2019-07-18 President And Fellows Of Harvard College Éditeurs de bases à haut rendement comprenant une gam
CA3082251A1 (fr) 2017-10-16 2019-04-25 The Broad Institute, Inc. Utilisations d'editeurs de bases adenosine
EP3724214A4 (fr) 2017-12-15 2021-09-01 The Broad Institute Inc. Systèmes et procédés de prédiction des résultats de la réparation en ingénierie génétique
SG11202007382TA (en) * 2018-02-15 2020-08-28 Sigma Aldrich Co Llc Engineered cas9 systems for eukaryotic genome modification
US12084676B2 (en) 2018-02-23 2024-09-10 Pioneer Hi-Bred International, Inc. Cas9 orthologs
WO2019226953A1 (fr) 2018-05-23 2019-11-28 The Broad Institute, Inc. Éditeurs de bases et leurs utilisations
CA3104856A1 (fr) 2018-06-29 2020-01-02 Editas Medicine, Inc. Molecules de guidage synthetiques, compositions et procedes associes
US12203123B2 (en) 2018-10-01 2025-01-21 North Carolina State University Recombinant type I CRISPR-Cas system and uses thereof for screening for variant cells
EP3861120A4 (fr) 2018-10-01 2023-08-16 North Carolina State University Système crispr-cas de type i recombinant
US12264330B2 (en) 2018-10-01 2025-04-01 North Carolina State University Recombinant type I CRISPR-Cas system and uses thereof for killing target cells
US12264313B2 (en) 2018-10-01 2025-04-01 North Carolina State University Recombinant type I CRISPR-Cas system and uses thereof for genome modification and alteration of expression
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
AU2019398351A1 (en) 2018-12-14 2021-06-03 Pioneer Hi-Bred International, Inc. Novel CRISPR-Cas systems for genome editing
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
DE112020001306T5 (de) 2019-03-19 2022-01-27 Massachusetts Institute Of Technology Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN110117622B (zh) * 2019-05-24 2021-05-11 青岛农业大学 一种CRISPR/Cas基因编辑系统及其制备方法和应用
WO2020263773A1 (fr) * 2019-06-24 2020-12-30 North Carolina State University Procédés et compositions pour modifier des génomes bactériens et pour tuer des bactéries
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN115176001A (zh) * 2019-12-11 2022-10-11 因特利亚治疗公司 用于基因编辑的修饰的引导rna
US20230332218A1 (en) * 2020-04-21 2023-10-19 Mammoth Biosciences, Inc. Casy programmable nucleases and rna component systems
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013176772A1 (fr) * 2012-05-25 2013-11-28 The Regents Of The University Of California Procédés et compositions permettant la modification de l'adn cible dirigée par l'arn et la modulation de la transcription dirigée par l'arn

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013188638A2 (fr) 2012-06-15 2013-12-19 The Regents Of The University Of California Endoribonucléases et leurs procédés d'utilisation
EP3097212A4 (fr) * 2014-01-24 2017-10-04 North Carolina State University Méthodes et compositions pour des séquences guidant le ciblage de cas9

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013176772A1 (fr) * 2012-05-25 2013-11-28 The Regents Of The University Of California Procédés et compositions permettant la modification de l'adn cible dirigée par l'arn et la modulation de la transcription dirigée par l'arn

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CONG ET AL.: "Multiplex genome engineering using CRISPR/Cas systems.", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 819 - 823, XP055181426 *

Also Published As

Publication number Publication date
JP2020103295A (ja) 2020-07-09
EP3097212A2 (fr) 2016-11-30
JP2022106883A (ja) 2022-07-20
JP7429057B2 (ja) 2024-02-07
EP3097212A4 (fr) 2017-10-04
CA2936646A1 (fr) 2015-07-30
WO2015112896A2 (fr) 2015-07-30
JP2017503514A (ja) 2017-02-02
JP2024041934A (ja) 2024-03-27
CA2936646C (fr) 2024-04-30
WO2015112896A9 (fr) 2015-11-26

Similar Documents

Publication Publication Date Title
WO2015112896A3 (fr) Méthodes et compositions pour des séquences guidant le ciblage de cas9
EP3374494A4 (fr) Compositions crispr et leurs méthodes d'utilisation pour la thérapie génique
PH12018501378A1 (en) Compositions and methods for the treatment of hemoglobinopathies
PH12017500858A1 (en) Mocrocyclic peptides useful as immunomodulators
ZA201707749B (en) Compounds targeting proteins, compositions, methods, and uses thereof
EA039598B9 (ru) Соединение, нацеленное на ил-23а и фно-альфа, и его применение
EP3298169A4 (fr) Compositions et procédés pour l'enrichissement de populations d'acides nucléiques
EP3183367A4 (fr) Compositions et méthodes pour l'enrichissement d'acides nucléiques
EP3234200A4 (fr) Compositions et procédés d'appauvrissement ciblé, d'enrichissement et de séparation d'acides nucléiques utilisant les protéines du système cas/crispr
EP3633032A3 (fr) Nouvelles protéines cas9 et éléments de guidage pour le ciblage de l'adn et l'édition du génome
LT3204015T (lt) Kompozicijos ir būdai, skirti hao1 (hidroksirūgšties oksidazė 1 (glikolato oksidazė)) genų raiškos slopinimui
EP3750907A3 (fr) Compositions à base de raav et procédés pour traiter la sclérose latérale amyotrophique
ZA201803723B (en) Compositions and methods for efficient targeting of transgenes
PH12018501279A1 (en) Compositions comprising 15-hepe and methods of using the same
MX2019004156A (es) Variantes de endonucleasa homing de receptor de linfocitos t alfa (tcralpha).
EP3209675A4 (fr) Compositions et procédés pour la détection d'acides nucléiques
WO2016094374A8 (fr) Compositions et méthodes de traitement de l'ataxie de friedreich
MX2018005872A (es) Profarmacos de acido nucleico.
MX2024000004A (es) Proteinas de fusion de inhibidor de tripsina urinaria (uti).
HK1255221A1 (zh) 使用卡多曲组合物进行治疗的方法
PH12016501326A1 (en) (s)-3'-methyl-abscisic acid and esters thereof
EP3191186A4 (fr) Compositions et procédés d'utilisation de l'il4i1 (interleukine-4 induced gene 1)
WO2017106683A3 (fr) Molécules d'arn concatémères, compositions, et leurs procédés et utilisations
EP3271326A4 (fr) Compositions pour le traitement d'une maladie hépatique et/ou rénale
HK1261224A1 (en) Crispr compositions and methods of using the same for gene therapy

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15740979

Country of ref document: EP

Kind code of ref document: A2

DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
ENP Entry into the national phase

Ref document number: 2936646

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2016547162

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 15113656

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

REEP Request for entry into the european phase

Ref document number: 2015740979

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2015740979

Country of ref document: EP

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15740979

Country of ref document: EP

Kind code of ref document: A2