[go: up one dir, main page]

WO2015111293A1 - Système de tri de particules et procédé de tri de particules - Google Patents

Système de tri de particules et procédé de tri de particules Download PDF

Info

Publication number
WO2015111293A1
WO2015111293A1 PCT/JP2014/080587 JP2014080587W WO2015111293A1 WO 2015111293 A1 WO2015111293 A1 WO 2015111293A1 JP 2014080587 W JP2014080587 W JP 2014080587W WO 2015111293 A1 WO2015111293 A1 WO 2015111293A1
Authority
WO
WIPO (PCT)
Prior art keywords
pipe
particle sorting
vibrating body
sheath
vibration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2014/080587
Other languages
English (en)
Japanese (ja)
Inventor
秋山　昭次
隆志 宮田
河西　弘人
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sony Corp
Original Assignee
Sony Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sony Corp filed Critical Sony Corp
Publication of WO2015111293A1 publication Critical patent/WO2015111293A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1406—Control of droplet point

Definitions

  • This technology relates to a particle sorting apparatus and a particle sorting method. More specifically, the present invention relates to a technique for separating and collecting particles based on the result of analysis by an optical method or the like.
  • a flow cytometer is a device that irradiates light on the microparticles that flow through a flow channel formed in a flow cell, a microchip, etc., and detects and analyzes fluorescence and scattered light emitted from the individual microparticles. .
  • Some flow cytometers have the function of sorting and collecting only particles with specific characteristics based on the analysis results, and the device that specifically sorts cells is called the “cell sorter”. .
  • this cell sorter generally, a fluid discharged from the flow path is made into droplets by applying vibration to a flow cell or a microchip by a vibrating element or the like (see Patent Documents 1 and 2 and Non-Patent Document 1).
  • the main object of the present disclosure is to provide a particle sorting apparatus and a particle sorting method that can stably form a droplet having a good shape even when a fluid is vibrated at a high frequency.
  • a particle sorting apparatus includes a pipe that introduces at least a sheath liquid into a flow path that communicates with an orifice that generates a fluid stream, and a vibrating body that applies vibration to the pipe.
  • a droplet containing particles is ejected.
  • the channel may be formed in the microchip.
  • the pipe is, for example, a sheath pipe through which the sheath liquid flows or a merging pipe through which the sheath liquid flows around the sample liquid containing the particles.
  • the vibrating body is, for example, a piezoelectric element. In that case, the vibration exciter may be disposed in contact with the pipe.
  • the vibrating body may be fixed to the pipe via an adhesive.
  • the vibration exciter may have a through hole, and the pipe may be inserted through the through hole.
  • high-frequency vibration of 1 kHz or more can be applied to the pipe by the vibrating body.
  • the vibrating body imparts vibration in a direction perpendicular or parallel to the flow direction of the liquid flowing through the pipe, for example.
  • vibration is applied to at least a pipe that introduces sheath liquid into a flow path that communicates with an orifice that generates a fluid stream, and a droplet containing particles is discharged from the orifice.
  • FIGS. 2A and 2B are views showing a method of attaching the vibrating body 3 shown in FIG. A and B are figures which show the other attachment method of the vibrating body 3 shown in FIG. 1, A is sectional drawing, B is a top view.
  • a and B are figures which show the other attachment method of the vibrating body 3 shown in FIG. 1, A is sectional drawing, B is a top view.
  • FIG. 1 is a schematic diagram illustrating a configuration of a particle sorting apparatus according to a first embodiment of the present disclosure.
  • the particle sorting apparatus 1 sorts and collects particles based on the result of analysis by an optical technique or the like, and as shown in FIG. 1, the microchip 2, the vibrating body 3, and the deflection plate 5a, 5b, recovery containers 6a to 6c, and the like.
  • the particles analyzed and sorted by the particle sorting apparatus 1 of the present embodiment include biologically related fine particles such as cells, microorganisms and ribosomes, or synthetic particles such as latex particles, gel particles and industrial particles. included.
  • Biologically relevant microparticles include chromosomes, ribosomes, mitochondria, organelles (cell organelles), etc. that make up various cells.
  • the cells include plant cells, animal cells, blood cells, and the like.
  • microorganisms include bacteria such as Escherichia coli, viruses such as tobacco mosaic virus, and fungi such as yeast.
  • the biologically relevant microparticles may include biologically relevant polymers such as nucleic acids, proteins, and complexes thereof.
  • examples of the industrial particles include those formed of an organic polymer material, an inorganic material, or a metal material.
  • organic polymer material polystyrene, styrene / divinylbenzene, polymethyl methacrylate, or the like can be used.
  • inorganic material glass, silica, a magnetic material, etc. can be used.
  • metal material for example, gold colloid and aluminum can be used.
  • shape of these fine particles is generally spherical, it may be non-spherical, and the size and mass are not particularly limited.
  • the microchip 2 includes a sample inlet 22 into which a liquid (sample liquid) containing particles to be sorted is introduced, a sheath inlet 23 into which a sheath liquid is introduced, a suction outlet 24 for eliminating clogging and bubbles, and the like. Is formed.
  • the sample liquid is introduced into the sample inlet 22, merged with the sheath liquid introduced into the sheath inlet 23, and sent to the sample flow path, and the orifice 21 provided at the end of the sample flow path. It is discharged from.
  • a suction channel communicating with the suction outlet 24 is connected to the sample channel.
  • This suction channel is used to eliminate clogging and air bubbles by creating a negative pressure in the sample channel and temporarily reversing the flow when clogging or air bubbles occur in the sample channel.
  • a negative pressure source such as a vacuum pump is connected to 24.
  • the microchip 2 can be formed of glass or various plastics (PP, PC, COP, PDMS, etc.).
  • the material of the microchip 1 is desirably a material that is transparent to the measurement light emitted from the light detection unit, has less autofluorescence, and has less optical error due to small wavelength dispersion.
  • the microchip 2 can be formed by wet etching or dry etching of a glass substrate, or by nanoimprinting, injection molding, or machining of a plastic substrate.
  • the microchip 2 can be formed, for example, by sealing a substrate formed with a sample flow path or the like with a substrate of the same material or a different material.
  • the vibrating body 3 applies vibration to the liquid flowing through the microchip 2, converts the fluid discharged from the orifice 21 into droplets, and generates a fluid stream (droplet flow) S.
  • a fluid stream droplet flow
  • the vibrator 3 is not particularly limited as long as it can vibrate the pipe, but a piezoelectric element is preferable from the viewpoint of controllability and size.
  • FIGS. 2 to 5 are diagrams schematically showing how to attach the vibrating body 3.
  • the vibrating body 3 is not particularly limited as long as vibration can be applied to at least a pipe for introducing the sheath liquid into the flow path provided in the microchip 2.
  • the vibrating body 3 is a piezoelectric element, as shown in FIGS.
  • FIGS. 4 and 5 for example, by using a piezoelectric element 16 having a cylindrical through hole 16 a and inserting the pipe 14 into the through hole 16 a, the entire circumferential direction of the pipe 14 is extended. It is good also as a structure by which the piezoelectric element 16 is arrange
  • the installation method of the vibrating body 3 is not particularly limited as long as it is installed in a state where vibration can be applied to a predetermined pipe.
  • the vibrating body 3 is a piezoelectric element
  • it is disposed so as to contact the pipe 14 as shown in FIGS.
  • the piezoelectric elements 13 and 16 may be fixed to the pipe 14 via an adhesive as shown in FIGS. 2 and 4, and the fixing members 15a to 15c are used as shown in FIGS. It can also be fixed, and may be fixed in combination.
  • the pipe for applying vibration by the vibrating body 3 is not limited to the sheath pipe 4, and may be any pipe that introduces at least the sheath liquid into the flow path communicating with the orifice 21.
  • the vibrator 3 may be attached to the merge pipe through which the joined liquid flows. Since the sample liquid has a smaller flow rate than the sheath liquid, when only the sample pipe for introducing the sample liquid is vibrated, the fluid discharged from the orifice 21 may not be formed into droplets.
  • the pipe for applying vibration by the vibrating body 3 is formed of a relatively hard material from the viewpoint of excitation efficiency.
  • the piping since the piping may be sterilized and washed with ethanol, hypochlorous acid, or the like, it is preferable that the piping be formed of a material having excellent chemical resistance. Examples of such a piping material include PEEK (polyetheretherketone) resin.
  • the charging unit (not shown) applies positive or negative charges to the droplets ejected from the orifice 21, and includes a charge electrode and a voltage source that applies a predetermined voltage to the electrode. ing.
  • the charging electrode is disposed in contact with the sheath liquid and / or sample liquid flowing in the flow path, and applies charge to the sheath liquid and / or sample liquid. For example, the charging electrode is applied to the charged electrode inlet of the microchip 2. Inserted.
  • the deflecting plates 5a and 5b change the traveling direction of each droplet in the fluid stream S by an electric force acting between the electric charges applied to the droplet and guide it to a predetermined recovery container. Yes, with the fluid stream S interposed therebetween.
  • the deflection plates 5a and 5b for example, commonly used electrodes can be used.
  • a different positive or negative voltage is applied to each of the deflecting plates 5a and 5b, and when a charged droplet passes through the electric field formed thereby, an electric force (Coulomb force) is generated, and each liquid Drops are attracted in the direction of one of the deflecting plates 5a and 5b.
  • the collection containers 6a to 6c collect droplets that have passed between the deflection plates 5a and 5b, and general-purpose plastic tubes or glass tubes can be used for experiments. These collection containers 6a to 6c are preferably arranged so as to be replaceable in the apparatus. Further, a drainage path for the collected droplets may be connected to the collection containers 6a to 6c that receive non-target minute particles.
  • the number of the collection containers arranged in the fine particle sorting apparatus 1 is not particularly limited. For example, when more than three collection containers are arranged, each droplet is guided to one of the collection containers depending on the presence / absence of the electric force between the deflecting plates 5a and 5b and the size thereof. It can be recovered.
  • the particle sorting apparatus 1 of the present embodiment irradiates, for example, light (measurement light) to a predetermined portion of the sample flow path, and emits light (measurement target light) generated from fine particles flowing through the sample flow path.
  • a light detection unit (not shown) for detection is provided. The light detection unit can be configured in the same manner as in conventional flow cytometry.
  • a laser light source an irradiation system consisting of a condensing lens, dichroic mirror, bandpass filter, etc. that collects and irradiates laser light on fine particles, and measurement generated from fine particles by laser light irradiation And a detection system for detecting the target light.
  • the detection system includes, for example, a PMT (Photo Multiplier Tube), an area image sensor such as a CCD or a CMOS element.
  • the irradiation system and the detection system may be configured by the same optical path or may be configured by separate optical paths.
  • the measurement target light detected by the detection system of the light detection unit is light generated from the microparticles by irradiation of the measurement light, such as forward scattered light, side scattered light, Rayleigh scattering, and Mie scattering. It can be scattered light or fluorescence. These measurement target lights are converted into electrical signals, and the optical characteristics of the microparticles are detected based on the electrical signals.
  • the sample liquid containing the particles to be sorted is introduced from the sample pipe connected to the sample inlet 22 and connected to the sheath inlet 23.
  • a sheath liquid is introduced from the sheath pipe 4.
  • the sheath pipe 4 is vibrated by the vibrating body 3 to impart vibration to the sheath liquid introduced into the microchip 2.
  • the direction of applying vibration by the vibration exciter 3 may be either a vertical direction or a parallel direction with respect to the flow direction of the liquid flowing in the pipe. Is preferred.
  • the piezoelectric element 16 expands and contracts in the longitudinal direction of the pipe 14, and therefore parallel to the liquid flow direction. Vibration is applied to the.
  • the piezoelectric elements 13 are arranged opposite to each other with the piping 14 shown in FIGS. 2 and 3, the piezoelectric elements 13 expand and contract in the direction perpendicular to the piping 14, so Thus, vibration is applied in the vertical direction. In this case, the excitation efficiency by the piezoelectric element is higher in the radial contraction than in the longitudinal expansion and contraction.
  • the detection of the optical characteristics of the particles and the detection of the flow rate (flow velocity) of the particles and the interval of the particles are performed by the light detection unit, for example.
  • the detected optical characteristics, flow velocity, interval, and the like of the detected particles are converted into electrical signals and output to the overall control unit (not shown) of the apparatus.
  • the laminar flow of the sample liquid and the sheath liquid that has passed through the light irradiation part of the sample flow path is discharged from the orifice 21 to the space outside the microchip 2.
  • at least the sheath liquid is vibrated by the vibrating body 3, so that the fluid discharged from the orifice 21 is turned into droplets.
  • each droplet charged in the sample channel is changed in its traveling direction by the deflecting plates 5a and 5b based on the detection result in the light detection unit, and is guided to the predetermined collection containers 6a to 6c to be collected. Is done.
  • the particle sorting apparatus of this embodiment vibrates the piping, it can form droplets without receiving the influence of resonance between the members and the shape of the microchip and its peripheral members. Thereby, even when a high frequency vibration of 1 kHz or more is applied, a droplet having a good shape can be stably formed.
  • the particle sorting apparatus of the present embodiment is suitable when vibrating in the range of 1 kHz to 200 kHz, and particularly preferably in the range of 10 kHz to 100 kHz.
  • FIG. 6 is a schematic diagram illustrating a configuration of a particle sorting apparatus according to the second embodiment of the present disclosure.
  • the same components as those of the particle sorting apparatus 1 of the first embodiment shown in FIG. 6 are the same components as those of the particle sorting apparatus 1 of the first embodiment shown in FIG.
  • the particle sorting device 11 of the present embodiment sorts and collects particles based on the result of analysis by an optical method or the like, and uses a flow cell 12 instead of a microchip.
  • the particle sorting apparatus of the first embodiment is the same as that described above.
  • a vibrating body 3 for applying vibration is attached to at least a pipe for introducing a sheath liquid into a flow path communicating with an orifice 31 provided in the flow cell 12.
  • the flow cell 12 includes a sample inlet 32 into which a liquid (sample liquid) containing particles to be sorted is introduced, a sheath inlet 33 into which a sheath liquid is introduced, and the like.
  • the sample liquid is introduced into the sample inlet 32, merged with the sheath liquid introduced into the sheath inlet 33, and then discharged from the orifice 31.
  • a sample liquid containing the particles to be sorted is introduced from the sample pipe 7 connected to the sample inlet 32 and connected to the sheath inlet 33.
  • the sheath liquid is introduced from the sheath pipe 4.
  • the sheath pipe 4 is vibrated by the vibrating body 3 to impart vibration to the sheath liquid introduced into the flow cell 12.
  • the detection of the optical characteristics of the particles and the detection of the flow rate (flow velocity) of the particles and the interval of the particles are performed by the light detection unit, for example.
  • the detected optical characteristics, flow velocity, interval, and the like of the detected particles are converted into electrical signals and output to the overall control unit (not shown) of the apparatus.
  • the laminar flow of the sample liquid and the sheath liquid that has passed through the light irradiation part of the sample flow path is discharged from the orifice 31 to the space outside the flow cell 12. At that time, at least the sheath liquid is vibrated by the vibrating body 3, so that the fluid discharged from the orifice 31 is turned into droplets.
  • Each droplet charged in the sample channel is changed in its traveling direction by the deflecting plates 5a and 5b based on the detection result in the light detection unit, and is guided to the predetermined collection containers 6a to 6c and collected. .
  • the vibrating body is attached to the flow cell, not only the fluid but also the flow cell and its peripheral members vibrate. For this reason, resonance occurs between the members, and the droplet shape is likely to vary due to the influence.
  • the vibration is applied to the pipe, so the resonance between the members. Can be suppressed, and the droplet shape can be stabilized.
  • the configuration and effects other than those described above in the microparticle sorting apparatus of the present embodiment are the same as those of the first embodiment described above.
  • the flow cell is more expensive than the microchip, and if the nozzle is clogged, the flow cell needs to be replaced greatly.
  • it is extremely difficult to completely sterilize an apparatus using a flow cell because it requires long-time washing in order to eliminate contamination with particles used in the previous measurement.
  • it is preferable to use a microchip rather than a flow cell and it is preferable to use an apparatus using a microchip, particularly when it is necessary to perform measurement under aseptic conditions.
  • the present disclosure can take the following configurations.
  • the pipe is a sheath pipe through which a sheath liquid flows or a joining pipe through which a sheath liquid flows around a sample liquid containing the particles.
  • the vibrating body is a piezoelectric element.
  • the particle sorting apparatus according to any one of (1) to (8), wherein the vibration exciter imparts vibration in a direction perpendicular to a flow direction of the liquid flowing through the pipe.
  • the particle sorting device according to any one of (1) to (8), wherein the vibration exciter imparts vibration in a direction parallel to a flow direction of the liquid flowing through the pipe.
  • (11) A particle sorting method in which vibration is applied to at least a pipe for introducing sheath liquid into a flow path communicating with an orifice that generates a fluid stream by a vibrating body, and droplets containing particles are discharged from the orifice.
  • two piezoelectric elements (20 m type manufactured by NEC TOKIN Corporation) were used as the vibrator, and a microchip having an orifice diameter of 85 ⁇ m was used.
  • the driving voltage of the piezoelectric element was 3.6 V
  • the driving frequency was about 40 kHz
  • the frequency at which the droplet generated from the tip of the chip (Break off length: BOL) was the shortest was selected.
  • the measurement was performed under two conditions: sheath pressure 30 psi (sheath fluid flow rate: about 15 m / second) and sheath pressure 35 psi (sheath fluid flow rate: about 17 m / second).
  • FIGS. 7A and 7B are results when the sheath pressure is 30 psi
  • FIGS. 8A and 8B are results when the sheath pressure is 35 psi.
  • FIGS. 7A and 8A are views showing the state of droplets formed by the particle sorting apparatus of the embodiment
  • FIGS. 7B and 8B are the states of droplets formed by the conventional particle sorting apparatus.
  • FIG. 7B and 8B Although the droplets D formed by the conventional particle sorting apparatus shown in FIGS. 7B and 8B vary in shape, the particle sorting apparatus of the embodiment in which the sheath pipe shown in FIGS. 7A and 8A is vibrated. The droplet D formed by the above was excellent in shape stability.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)

Abstract

La présente invention concerne un système de tri de particules et un procédé de tri de particules avec lesquels il est possible de former de façon stable des gouttelettes possédant des formes satisfaisantes même lorsque le fluide est mis à vibrer à une fréquence élevée. Dans le système de tri de particules, les gouttelettes contenant les particules sont éjectées par un orifice qui produit un courant de fluide, et des vibrations sont appliquées par un vibreur à un pipeline à travers lequel au moins un fluide de gaine est introduit dans un canal qui communique avec l'orifice.
PCT/JP2014/080587 2014-01-24 2014-11-19 Système de tri de particules et procédé de tri de particules Ceased WO2015111293A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2014-011724 2014-01-24
JP2014011724 2014-01-24

Publications (1)

Publication Number Publication Date
WO2015111293A1 true WO2015111293A1 (fr) 2015-07-30

Family

ID=53681115

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2014/080587 Ceased WO2015111293A1 (fr) 2014-01-24 2014-11-19 Système de tri de particules et procédé de tri de particules

Country Status (1)

Country Link
WO (1) WO2015111293A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022024477A1 (fr) * 2020-07-28 2022-02-03 ソニーグループ株式会社 Dispositif de dosage de microparticules et procédé de dosage de microparticules
JPWO2022024575A1 (fr) * 2020-07-28 2022-02-03

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63259466A (ja) * 1987-04-16 1988-10-26 Hitachi Ltd 細胞分析装置
JPH08297121A (ja) * 1995-04-26 1996-11-12 Hitachi Ltd 粒子分析装置
JPH10507524A (ja) * 1994-10-14 1998-07-21 ユニバーシティ オブ ワシントン 高速フローサイトメータ液滴形成システム
US5819948A (en) * 1997-08-21 1998-10-13 Van Den Engh; Gerrit J. Particle separating apparatus and method
JP2004184217A (ja) * 2002-12-03 2004-07-02 Bay Bioscience Kk 生物学的粒子の情報を得る装置
JP2008143105A (ja) * 2006-12-12 2008-06-26 Idemitsu Kosan Co Ltd 樹脂への超音波振動付与装置、この超音波振動付与装置を用いて製造した樹脂組成物
WO2008153056A1 (fr) * 2007-06-14 2008-12-18 Mitsui Engineering & Shipbuilding Co., Ltd. Cytomètre de flux doté d'une fonction de fractionnement des cellules et procédé de fractionnement de cellules vivantes
JP2010181189A (ja) * 2009-02-03 2010-08-19 Mitsui Eng & Shipbuild Co Ltd フローサイトメータおよびサンプル測定方法
JP2010190680A (ja) * 2009-02-17 2010-09-02 Sony Corp 微小粒子分取のための装置及びマイクロチップ
JP2011025148A (ja) * 2009-07-24 2011-02-10 Hitachi Plant Technologies Ltd 化学生産装置

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63259466A (ja) * 1987-04-16 1988-10-26 Hitachi Ltd 細胞分析装置
JPH10507524A (ja) * 1994-10-14 1998-07-21 ユニバーシティ オブ ワシントン 高速フローサイトメータ液滴形成システム
JPH08297121A (ja) * 1995-04-26 1996-11-12 Hitachi Ltd 粒子分析装置
US5819948A (en) * 1997-08-21 1998-10-13 Van Den Engh; Gerrit J. Particle separating apparatus and method
JP2004184217A (ja) * 2002-12-03 2004-07-02 Bay Bioscience Kk 生物学的粒子の情報を得る装置
JP2008143105A (ja) * 2006-12-12 2008-06-26 Idemitsu Kosan Co Ltd 樹脂への超音波振動付与装置、この超音波振動付与装置を用いて製造した樹脂組成物
WO2008153056A1 (fr) * 2007-06-14 2008-12-18 Mitsui Engineering & Shipbuilding Co., Ltd. Cytomètre de flux doté d'une fonction de fractionnement des cellules et procédé de fractionnement de cellules vivantes
JP2010181189A (ja) * 2009-02-03 2010-08-19 Mitsui Eng & Shipbuild Co Ltd フローサイトメータおよびサンプル測定方法
JP2010190680A (ja) * 2009-02-17 2010-09-02 Sony Corp 微小粒子分取のための装置及びマイクロチップ
JP2011025148A (ja) * 2009-07-24 2011-02-10 Hitachi Plant Technologies Ltd 化学生産装置

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022024477A1 (fr) * 2020-07-28 2022-02-03 ソニーグループ株式会社 Dispositif de dosage de microparticules et procédé de dosage de microparticules
JPWO2022024575A1 (fr) * 2020-07-28 2022-02-03
WO2022024575A1 (fr) * 2020-07-28 2022-02-03 ソニーグループ株式会社 Analyseur de particules fines, système d'isolation de particules fines, et procédé d'analyse de particules fines
JPWO2022024477A1 (fr) * 2020-07-28 2022-02-03
CN116134304A (zh) * 2020-07-28 2023-05-16 索尼集团公司 微粒分选装置及微粒分选方法
JP7694568B2 (ja) 2020-07-28 2025-06-18 ソニーグループ株式会社 微小粒子分取装置及び微小粒子分取方法

Similar Documents

Publication Publication Date Title
JP6065527B2 (ja) 微小粒子分取装置及び微小粒子分取方法
JP6958650B2 (ja) 液滴分取装置、液滴分取方法及びプログラム
JP6136843B2 (ja) 粒子分取装置、粒子分取方法及びプログラム
JP5487638B2 (ja) 微小粒子分取のための装置及びマイクロチップ
JP6304034B2 (ja) 微小粒子分取装置、微小粒子分取方法及びプログラム
CN103717308B (zh) 微芯片和微粒分析装置
JP4572973B2 (ja) マイクロチップ及びマイクロチップにおける送流方法
JP6102783B2 (ja) 粒子分取装置、粒子分取方法及びプログラム
JP6447506B2 (ja) 粒子分取装置及び粒子分取方法
JP6311312B2 (ja) 微小粒子測定装置及び微小粒子測定装置における送液方法
JP2011237201A (ja) 微小粒子分取装置、マイクロチップ及びマイクロチップモジュール
WO2015111293A1 (fr) Système de tri de particules et procédé de tri de particules
JP2019063798A (ja) 粒子分取装置、粒子分取方法及びプログラム
JP2011064706A (ja) マイクロチップとその流路構造
CN116134304A (zh) 微粒分选装置及微粒分选方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14879707

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 14879707

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: JP