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WO2015104797A1 - Dispositif de tri de spermatozoïdes mobiles - Google Patents

Dispositif de tri de spermatozoïdes mobiles Download PDF

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Publication number
WO2015104797A1
WO2015104797A1 PCT/JP2014/050099 JP2014050099W WO2015104797A1 WO 2015104797 A1 WO2015104797 A1 WO 2015104797A1 JP 2014050099 W JP2014050099 W JP 2014050099W WO 2015104797 A1 WO2015104797 A1 WO 2015104797A1
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WO
WIPO (PCT)
Prior art keywords
motile sperm
sorting device
semen
motile
shape
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2014/050099
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English (en)
Japanese (ja)
Inventor
鈴木　保彦
彩 大谷
一晴 丹羽
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Menicon Co Ltd
Original Assignee
Menicon Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Menicon Co Ltd filed Critical Menicon Co Ltd
Priority to JP2015556657A priority Critical patent/JP6161728B2/ja
Priority to PCT/JP2014/050099 priority patent/WO2015104797A1/fr
Publication of WO2015104797A1 publication Critical patent/WO2015104797A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting

Definitions

  • the present invention relates to a motile sperm sorting device.
  • motile sperm In in vitro fertilization in the assisted reproduction technology (ART), it is important to use highly motile sperm (hereinafter referred to as motile sperm).
  • a method for selecting such motile spermatozoa a method using centrifugal separation can be mentioned. This method is excellent in that motile spermatozoa can be recovered with high yield, but there is a concern that the sperm is physically damaged by centrifuging because sperm is subjected to great gravity by centrifugation.
  • a motile sperm sorting device 200 used in the method of Patent Document 2 includes a bottom surface 21 having a circular opening 10, a side wall 22 provided on the periphery of the bottom surface 21, and the opening.
  • positioning part 20 divided by the partition 23 provided in the periphery of the part 10 and the motile sperm concentrating part 30 formed in the bottomed cylinder shape by using this opening part 10 as an upper end are provided.
  • the semen to be processed is placed in the semen placement portion 20, and a sorting medium such as a culture solution is overlaid on the semen placement portion 20 to a position beyond the partition wall 23. Since the sperm has a property of moving along the wall, by leaving it in this state for a predetermined time, the motile sperm can move over the partition wall 23 and move to the motile sperm concentration part 30.
  • the motile sperm can be selectively collected.
  • this method also has a problem that the amount of motile sperm collected is not sufficient, and its use is limited to microinsemination.
  • the present invention provides a motile sperm sorting device that can collect motile sperm at a concentration and volume that can be applied to any of artificial, in vitro and microinsemination without physically damaging the sperm DNA.
  • the purpose is to provide.
  • the motile sperm sorting device includes a semen placement section defined by a bottom surface having an opening, a side wall provided at the periphery of the bottom surface, and a partition wall surrounding the part of the bottom so as to include the opening. And a motility sperm concentrating portion formed in a bottomed cylindrical shape with the opening as an upper end, and the circumference of the partition in a plan view shape is the circumference of the smallest circle including the plan view shape Longer.
  • the motile sperm sorting device of the present invention when the partition is formed in a circular shape in plan view by forming the partition so that the circumference of the shape in plan view is longer than the circumference of the smallest circle including the shape in plan view Compared with the above, the movement path (movement channel) of the motile sperm from the semen placement part to the motile sperm concentration part increases. As a result, more motile sperm can move to the motile sperm concentrator, so that motile sperm can be selected without physical damage to sperm DNA and with better efficiency than before. Can be recovered automatically.
  • the circumferential length of the partition wall in a plan view shape is preferably 1.5 to 10 times, more preferably 2 to 8 times, more preferably 2.5 times the circumference of the smallest circle including the planar view shape. Double to 5 times. If it is 1.5 times or more, recovery of motile sperm can be performed more suitably. On the other hand, if it exceeds 10 times, the shape of the partition wall becomes complicated, making it difficult to manufacture.
  • the partition wall is formed in a corrugated plate shape having irregularities in the circumferential direction.
  • the corrugated plate shape may be a shape having irregularities, and may be linear or curved.
  • the partition wall By forming the partition wall in this way, the peripheral length can be increased.
  • the sperm since the sperm has a property of moving toward a narrow place along the wall, it is easy to move (rise) along the partition wall by gathering in the recesses of the partition wall. As a result, the recovery efficiency of motile sperm can be improved. Only a part of the partition wall may be formed in a corrugated plate shape, or the entire partition wall may be formed in a corrugated plate shape.
  • the planar view shape of the partition is a concave polygon.
  • a concave polygon has at least one interior angle greater than 180 °, so that the outer portion of the interior angle is a recess.
  • the spermatozoa arranged in the semen arrangement part gather in the concave part of the partition and easily move along the partition.
  • the planar view shape of the partition walls is a concave polygon” means that the planar view shape is a concave polygon when viewed macroscopically. Therefore, each side forming the concave polygon may be a straight line or a curved line.
  • the concave polygon is preferably a radial shape from the viewpoints of recovery efficiency of motile sperm, ease of manufacture, device scale, and the like.
  • Specific examples include star-shaped polygons such as star-shaped pentagons, star-shaped hexagons, star-shaped heptagons, and star-shaped octagons, and four- to twelve-leaf, preferably six to ten-leaf multi-leaf shapes. it can.
  • the volume of the semen placement portion (the volume defined by the bottom surface, the side wall, and the upper end of the partition wall) is 1 mL to 5 mL, preferably 1.5 mL to 4 mL. With such a capacity, a sufficient amount of semen can be processed at a time, so that the processing efficiency can be improved.
  • the capacity of the motile sperm concentrator is 0.1 mL to 1 mL, preferably 0.3 mL to 0.6 mL. With such a capacity, it is possible to collect spermatozoa that have reached the motile sperm concentration part at an appropriate concentration.
  • the partition is formed to be 0.3 mm to 1 mm higher than the liquid surface when a specified amount of semen is disposed in the semen disposition portion.
  • semen can be placed in the semen placement portion of the motile sperm sorting device of the present invention up to a height of 0.3 mm to 1 mm lower than the upper end of the partition wall.
  • the motile sperm sorting device of the present invention has two or more of the partition walls and the motile sperm concentrating portions. With such a configuration, the movement path (movement channel) of the motile sperm from the semen placement part to the motile sperm concentration part can be increased.
  • the number of partition walls and motile sperm collection parts is preferably 5 or less from the viewpoint of ease of production.
  • the motile sperm concentrator has a shape that tapers downward. As described above, spermatozoa have the property of moving toward a narrow place along the wall. By forming the motile sperm concentrator in this way, the motile spermatozoa can easily collect in the deep part of the motile sperm concentrator. Become. Therefore, the sorting medium containing the motile sperm at a higher concentration can be collected by preferentially collecting the sorting medium in the deep part of the motile sperm concentration part.
  • the motile sperm sorting device of the present invention is formed so as to be independent.
  • the motile sperm sorting device of the present invention may comprise any suitable support means that allows the device itself to stand on its own. Thereby, operability can be improved.
  • the support means include support legs and pedestals.
  • the support means can typically be provided to connect to the side wall or the bottom surface.
  • the motile sperm sorting device of the present invention includes a lid that covers the upper end opening of the side wall. By providing the lid, it is possible to prevent contamination of impurities and the like.
  • the motile sperm sorting device of the present invention has a scale that is a measure of the amount of liquid disposed in the semen disposition portion on the outer surface of the side wall. By having a scale, workability can be improved.
  • At least part of the outer surface of the motile sperm sorting device of the present invention is writable or labelable. Thereby, troubles, such as mix-up of a processing sample, can be prevented.
  • the outer surface that is writable or labelable can be the outer surface of any part such as a lid, sidewalls, and the like.
  • motile sperm selection can be used to collect motile sperm at a concentration and liquid volume applicable to any of artificial insemination, in vitro fertilization, and microinsemination without physically damaging sperm DNA.
  • a device is provided.
  • FIG. 1 is a schematic perspective view of a motile sperm sorting device in one embodiment of the present invention.
  • FIG. FIG. 2 is a schematic sectional view taken along line AA of the motile sperm sorting device shown in FIG. 1.
  • FIG. 2 is a schematic plan view of the motile sperm sorting device shown in FIG. 1.
  • (A) to (d) are schematic plan views of modified examples of the motile sperm sorting device of the present invention. It is a schematic perspective view of the conventional motile sperm selection device.
  • FIG. 6 is a schematic sectional view taken along line BB of the motile sperm sorting device shown in FIG.
  • FIG. 6 is a schematic plan view of the motile sperm sorting device shown in FIG. 5.
  • FIG. 1 is a schematic perspective view of a motile sperm sorting device in one embodiment of the present invention
  • FIGS. 2 and 3 are respectively a schematic cross-sectional view of the motile sperm sorting device shown in FIG. It is a schematic plan view.
  • the motility sperm sorting device 100 includes a bottom surface 21 having an opening 10, a side wall 22 provided at the periphery of the bottom surface 21, and a semen that is partitioned by a partition wall 23 that surrounds a part of the bottom surface 21 so as to include the opening 10.
  • positioning part 20, the motile sperm concentration part 30 formed in the bottomed cylinder shape by making the opening part 10 into an upper end, and the support leg 40 which supports this bottom face 21 are provided.
  • the motile sperm sorting device 100 may further include a lid that covers the upper end opening of the side wall 22.
  • the bottom surface 21 has a perforated disk shape in which a circular opening 10 is provided at the center.
  • the diameter of the bottom surface 21 is, for example, 10 mm to 40 mm, preferably 12 mm to 30 mm.
  • the diameter of the opening 10 is, for example, 3 mm to 15 mm, preferably 5 mm to 10 mm. If the diameter of the bottom surface and the opening is within the above range, the height of the partition wall is appropriately set while making the volume of the semen placement portion sufficient for practical use (for example, 1 mL to 5 mL, preferably 1.5 mL to 4 mL). can do. Further, the semen placement operation and the motile sperm collection operation can be easily performed.
  • the side wall 22 is formed so as to rise vertically from the periphery of the bottom surface 21, and the upper end is opened.
  • the height of the side wall 22 is, for example, 10 mm to 40 mm. With such a height, the semen placement operation and the motile sperm collection operation can be easily performed. Moreover, it can be excellent in self-supporting stability.
  • the partition wall 23 is formed to rise vertically from the bottom surface 21 so as to surround the opening 10.
  • the height of the partition wall 23 is 4 mm to 12 mm, for example. With such a height, it is possible to secure a path length for selecting motile spermatozoa while making the capacity of the semen placement portion sufficiently practical.
  • the planar view shape of the partition wall 23 is a radial nine-leaf shape, and the bottom of the concave portion of the curved waveform pattern is located on the periphery of the opening 10 in the planar view.
  • the circumferential length of the partition wall 23 in a plan view shape is longer than the circumference of the smallest circle including the plan view shape (a circle indicated by a dotted line in FIG. 3), preferably 1.5 times to 10 times, more preferably 2 times.
  • the length is 8 to 8 times, more preferably 2.5 to 5 times.
  • planar view shape of the opening 10 and the planar view shape of the partition wall 23 are different from each other, but these planar shapes may be the same.
  • the motile sperm concentration part 30 is formed in a tapered bottomed cylindrical shape with the opening 10 as the upper end.
  • the depth of the motile sperm concentration part 30 is, for example, 5 mm to 25 mm, preferably 10 mm to 20 mm. With such a depth, the capacity of the motile sperm concentrator can be practically sufficient (for example, 0.1 mL to 1 mL, preferably 0.3 mL to 0.6 mL). Moreover, the collection operation
  • the support leg 40 is formed to extend in the vertical direction from the periphery of the bottom surface 21.
  • the height of the support leg 40 is such that the distance from the work surface to the bottom surface 21 is equal to or greater than the depth of the motile sperm concentration part 30 when the motile sperm sorting device is placed on the work table.
  • the support legs are formed along the periphery of the bottom surface.
  • a plurality of support legs may be provided so as to extend vertically or obliquely downward from the side wall or the bottom surface. .
  • thermoplastic resin examples include polyethylene (PE) such as high density polyethylene (HDPE), medium density polyethylene (MDPE), and low density polyethylene (LDPE), polypropylene (PP), polyvinylidene chloride, and polyvinyl chloride (PVC).
  • PE polyethylene
  • HDPE high density polyethylene
  • MDPE medium density polyethylene
  • LDPE low density polyethylene
  • PP polypropylene
  • PVC polyvinylidene chloride
  • PVC polyvinyl chloride
  • PTFE Polytetrafluoroethylene
  • PS polystyrene
  • PVAc polyvinyl acetate
  • Teflon registered trademark
  • ABS resin acrylonitrile butadiene styrene resin
  • AS resin acrylonitrile styrene resin
  • General purpose plastics such as (meth) acrylic resin, polyethylene terephthalate (PET); polyamide (PA), polyacetal (POM), polycarbonate (PC), modified polyphenylene ether (modified PPE), polybutylene terephthalate Engineering plastics (eg, PBT), glass fiber reinforced polyethylene terephthalate (GF-PET), and cyclic polyolefin (COP); polyphenylene sulfide (PPS), polytetrafluoroethylene (PTFE), polysulfone (PSF), polyethersal Super engineering plastics such as phon (PES), amorphous polyarylate (PAR), liquid crystal polymer (LCP), polyetheretherketone (PEEK), thermoplastic polyimide (PI), and polyamideimide (PAI) can be mentioned.
  • a photocurable resin can also be used.
  • the photocurable resin include an epoxy resin, an acrylic resin, and a silicone resin.
  • the motile sperm sorting device 100 can be manufactured by any suitable method.
  • the motile sperm concentration part 30 can be formed integrally with the bottom surface by causing the bottom surface 21 to protrude downward.
  • the motile sperm concentration part 30 may be formed by joining the opening part of the bottomed cylindrical container having the upper end as an opening to the opening part 10 of the perforated disk-like bottom surface 21.
  • layered modeling may be performed based on three-dimensional data by an optical modeling method. From the viewpoint of mass production, it can be preferably formed integrally using an injection molding method or the like.
  • the semen to be processed is placed in the semen placement section using a pipette, dropper, or the like.
  • the semen is disposed to a height that is 0.3 mm to 1 mm below the upper end of the partition wall.
  • the semen to be processed may be as-collected semen or may be semen after any appropriate treatment such as filtration, washing or dilution. From the viewpoint of avoiding physical damage to sperm DNA, those not subjected to centrifugation are preferred.
  • a medium for sorting sperm such as a culture solution and a washing solution, is gently layered on the semen so as not to disturb the interface.
  • the sorting medium is layered so that the liquid level exceeds the upper end of the partition wall, preferably about 5 mm to 20 mm (by this operation, the motile sperm collecting part is also filled with the sorting medium).
  • the motile sperm moves along the partition wall and collects in the motile sperm concentration part.
  • the standing time varies depending on the amount of semen placed in the semen placement part, but is generally 30 minutes to 180 minutes.
  • the sorting medium is recovered from the motile sperm collection part, preferably from the deepest part thereof, using a pipette or a dropper.
  • the collected sorting medium (hereinafter referred to as “recovered solution”) may contain motile sperm at a concentration applicable to artificial insemination.
  • FIG. 4A the partition wall 23 is formed so that its planar view shape is a six-leaf shape.
  • the partition 23 is formed so that the planar view shape may become a star-shaped regular octagon.
  • the partition 23 is formed so that the planar view shape may have a waveform pattern.
  • the partition 23 is formed so that the planar view shape may have a linear waveform pattern.
  • Example 1 A motile sperm sorting device having the form shown in FIGS. 1 to 3 was manufactured by stereolithography.
  • the resulting motile sperm sorting device has a bottom diameter of 28 mm, a side wall height of 25.5 mm, a septum height of 5 mm, an opening diameter of 8 mm, and a depth of the motile sperm concentrating portion of 12.5 mm.
  • the volume of the semen placement part was 2.3 mL
  • the capacity of the motile sperm collection part was 0.4 mL.
  • the peripheral length of the partition in plan view was 2.7 times the circumference of the smallest circle including the plan view.
  • a motile sperm sorting device having the form shown in FIG. 4A was manufactured by stereolithography.
  • the obtained motile sperm sorting device has a bottom diameter of 14.1 mm, a side wall height of 20.25 mm, a septum height of 5 mm, an opening diameter of 5 mm, and a depth of the motile sperm concentrating portion of 18 mm.
  • the volume of the semen placement portion was 0.4 mL
  • the volume of the motile sperm collection portion was 0.22 mL.
  • the peripheral length of the planar view shape (six-leaf shape) of the partition wall was 1.5 times the circumference of the smallest circle including the planar view shape.
  • Motile sperm sorting devices having the forms shown in FIGS. 5 to 7 were manufactured by stereolithography.
  • the resulting motile sperm sorting device has a bottom diameter of 13 mm, a side wall height of 20.25 mm, a septum height of 5 mm, an opening diameter of 5 mm, and a depth of the motile sperm concentrating portion of 18.5 mm.
  • the volume of the semen placement part was 0.4 mL, and the capacity of the motile sperm collection part was 0.22 mL.
  • the tip of the pipette was brought into contact with the deepest part of the motile sperm collecting part to recover 0.4 mL of the sorting medium.
  • the number of motile spermatozoa in the collected liquid was measured using a calculation board, and the concentration was calculated.
  • Test Example 2 Use of the motile sperm sorting device obtained in Example 2, 0.3 ml of human semen in the semen placement section, and recovery of 0.2 ml of the sorting medium from the motile sperm collection section Except for the above, motile sperm was collected in the same manner as in Test Example 1.
  • positioning part was a position 1 mm lower than the partition upper end.
  • the number of motile spermatozoa in the collected liquid was measured using a calculation board, and the concentration was calculated. The results are shown in Table 1.
  • Test Example 3 Motile sperm was collected in the same manner as in Test Example 1 except that human semen collected from different individuals was used. The number of motile spermatozoa in the collected liquid was measured using a calculation board, and the concentration was calculated. The results are shown in Table 2.
  • Test Example 4 Motile sperm was collected in the same manner as in Test Example 2, except that human semen collected from different individuals (the same human semen as used in Test Example 3) was used. The number of motile spermatozoa in the collected liquid was measured using a calculation board, and the concentration was calculated. The results are shown in Table 2.
  • Comparative Test Example 2 Motile spermatozoa were collected in the same manner as in Comparative Test Example 1 except that human semen collected from different individuals (the same human semen as used in Test Example 3) was used. The number of motile spermatozoa in the collected liquid was measured using a calculation board, and the concentration was calculated. The results are shown in Table 2.
  • the device of Comparative Example 1 It is possible to sort and collect motile spermatozoa with remarkably superior efficiency.
  • the device of Example 2 has 1.5 times the peripheral length of the planar shape of the partition wall as compared with the device of Comparative Example 1, but it is possible to recover the number and concentration of motile sperm of 5 times or more. it can.
  • the motile sperm sorting device of the present invention can be suitably used in the field of assisted reproduction medical technology.

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Abstract

La présente invention concerne un dispositif de tri de spermatozoïdes mobiles, comprenant : une section de dépôt de sperme séparée par un fond présentant une ouverture, une paroi latérale disposée au niveau du bord périphérique du fond et une paroi de séparation englobant une partie d'un fond de façon à comporter l'ouverture ; et une section de recueil de spermatozoïdes mobiles façonnée dans une forme cylindrique qui présente un fond avec ladite ouverture en tant que son bord supérieur. La longueur circonférentielle de la forme, vue en plan, de la paroi de séparation est plus longue que la longueur circonférentielle du plus petit cercle qui comporte la forme vue en plan.
PCT/JP2014/050099 2014-01-08 2014-01-08 Dispositif de tri de spermatozoïdes mobiles Ceased WO2015104797A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2015556657A JP6161728B2 (ja) 2014-01-08 2014-01-08 運動性精子選別デバイス
PCT/JP2014/050099 WO2015104797A1 (fr) 2014-01-08 2014-01-08 Dispositif de tri de spermatozoïdes mobiles

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PCT/JP2014/050099 WO2015104797A1 (fr) 2014-01-08 2014-01-08 Dispositif de tri de spermatozoïdes mobiles

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107099444A (zh) * 2017-04-17 2017-08-29 隗慧林 精子分选芯片、精子检测设备及精子检测方法
EP3559261A4 (fr) * 2016-12-23 2020-12-23 The Regents of The University of California Procédé et dispositif de fusion numérique haute résolution
US20210354142A1 (en) * 2020-05-15 2021-11-18 David Sinton Sperm selection device, kit, and methods
CN115701450A (zh) * 2021-08-02 2023-02-10 邦睿生技股份有限公司 精子分选装置及方法

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Publication number Priority date Publication date Assignee Title
JP2002510045A (ja) * 1998-03-30 2002-04-02 バイオシャフ リミテッド 細胞および体液における一般的診断要因を分析するためのフロー式細胞数計算器
JP2003289850A (ja) * 2002-04-05 2003-10-14 Nipro Corp 精子選択採取用シャーレ
US20050026274A1 (en) * 2001-10-05 2005-02-03 Josef Zech Device and method for selecting locomotive biological species, in particularly sperm cells
JP2006304683A (ja) * 2005-04-28 2006-11-09 Nipro Corp 精子選択採取用シャーレ
US20100075411A1 (en) * 2008-09-22 2010-03-25 Cecchi Michael D Specimen manipulation device for micro manipulation and biopsy in assisted reproduction and in vitro fertilization
WO2010122562A1 (fr) * 2009-04-22 2010-10-28 Lotus Bio (Nymphaea) Ltd. Système de séparation du sperme

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002510045A (ja) * 1998-03-30 2002-04-02 バイオシャフ リミテッド 細胞および体液における一般的診断要因を分析するためのフロー式細胞数計算器
US20050026274A1 (en) * 2001-10-05 2005-02-03 Josef Zech Device and method for selecting locomotive biological species, in particularly sperm cells
JP2003289850A (ja) * 2002-04-05 2003-10-14 Nipro Corp 精子選択採取用シャーレ
JP2006304683A (ja) * 2005-04-28 2006-11-09 Nipro Corp 精子選択採取用シャーレ
US20100075411A1 (en) * 2008-09-22 2010-03-25 Cecchi Michael D Specimen manipulation device for micro manipulation and biopsy in assisted reproduction and in vitro fertilization
WO2010122562A1 (fr) * 2009-04-22 2010-10-28 Lotus Bio (Nymphaea) Ltd. Système de séparation du sperme

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3559261A4 (fr) * 2016-12-23 2020-12-23 The Regents of The University of California Procédé et dispositif de fusion numérique haute résolution
US11915795B2 (en) 2016-12-23 2024-02-27 The Regents Of The University Of California Method and device for digital high resolution melt
CN107099444A (zh) * 2017-04-17 2017-08-29 隗慧林 精子分选芯片、精子检测设备及精子检测方法
CN107099444B (zh) * 2017-04-17 2024-04-09 隗慧林 精子分选芯片、精子检测设备及精子检测方法
US20210354142A1 (en) * 2020-05-15 2021-11-18 David Sinton Sperm selection device, kit, and methods
US12357991B2 (en) * 2020-05-15 2025-07-15 David Sinton Sperm selection device, kit, and methods
CN115701450A (zh) * 2021-08-02 2023-02-10 邦睿生技股份有限公司 精子分选装置及方法

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JPWO2015104797A1 (ja) 2017-03-23

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