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WO2015192261A1 - Système et procédé colorimétrique pour mesurer la teneur en sucres des vins et des jus - Google Patents

Système et procédé colorimétrique pour mesurer la teneur en sucres des vins et des jus Download PDF

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Publication number
WO2015192261A1
WO2015192261A1 PCT/CL2015/050019 CL2015050019W WO2015192261A1 WO 2015192261 A1 WO2015192261 A1 WO 2015192261A1 CL 2015050019 W CL2015050019 W CL 2015050019W WO 2015192261 A1 WO2015192261 A1 WO 2015192261A1
Authority
WO
WIPO (PCT)
Prior art keywords
blue
concentration
sugar
wells
colorimetric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CL2015/050019
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English (en)
Spanish (es)
Inventor
Marcos Caroli
Geraldine Mena
Nicolas Leiva
Maritza PÁEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Santiago de Chile
Original Assignee
Universidad de Santiago de Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Santiago de Chile filed Critical Universidad de Santiago de Chile
Publication of WO2015192261A1 publication Critical patent/WO2015192261A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/46—Measurement of colour; Colour measuring devices, e.g. colorimeters
    • G01J3/50—Measurement of colour; Colour measuring devices, e.g. colorimeters using electric radiation detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/14—Beverages

Definitions

  • the present invention relates to a colorimetric system and method for measuring the sugar content in wines and juices in general, in the field or in a production line, where colorimetric data is generated from images that have been previously captured and processed. , sending the image data, directly or remotely to an analytical center to determine the sugar content in the wine or juice sample.
  • the system and method have an accuracy equivalent to that of an analysis using an ultraviolet (UV) -visible spectrometer.
  • UV ultraviolet
  • the present invention has application in the centralized control of sugar content in wines and juices, in general, and comprises an analytical center connected to meters in the field or in a production line. It uses simple and cheap resources, such as a digital camera with network image transmission, which replace more elaborate, but much more expensive, heavy and fixed methods, such as colorimetric measurements through a UV-visible spectrometer. The replacement of more expensive and heavy spectrophotometric methods with colorimetric methods based on digital cameras is described in literature, in the color analysis of solutions of a solvatochromic indicator (F. Shakerizadeh-Shirazi, B. Hemmateene jad, AM Mehranpour, Anal. Methods , 2013, 5, 891-896).
  • a solvatochromic indicator F. Shakerizadeh-Shirazi, B. Hemmateene jad, AM Mehranpour, Anal. Methods , 2013, 5, 891-896.
  • Figure 1 Illustrates the components of the system of the invention: liquid samples of wine or juice treated with Dubois colorimetric reagents are deposited in one or more wells (A) inside a container, and their images captured after being illuminated by internal lighting means (B) in the container. The captured images are transferred directly or remotely, to an analytical center where a data processing medium, preferably a computer (C), analyzes each image according to contributions of Red (R), green (G) and blue (B) . The average of the contributions of blue for each image captured from the liquid sample is plotted against the corresponding sugar concentration, obtaining a calibration line (D), which allows determining the sugar content in samples with unknown sugar content.
  • a data processing medium preferably a computer (C)
  • R Red
  • G green
  • B blue
  • D calibration line
  • Figure 2 Illustrates a container provided with interior lighting means, preferably lamps; an image capture medium, preferably a camera digital, and one or more porcelain wells to deposit liquid samples.
  • Figure 3 Illustrates the use of the Color Man ⁇ a 3.0 program, which converts the color at the point indicated by the arrow (top, right) into contributions of red (R), green (G) and blue (B). The contributions of blue (B) for 5-10 points of each well are averaged, obtaining an average value for the concentration of sugar used. With this data, a calibration line is drawn that allows the concentration of sugar in unknown samples to be determined.
  • Figure 4 Illustrates an example of a calibration curve obtained from seven different glucose concentrations, in a range of 0-1%.
  • Figure 5 Illustrates a line for calibration of total sugar concentrations, according to the colorimetric method of the invention, used in the determination of the total sugar of the wine sample of example 1.
  • Figure 7 - Blue (B) intensity calibration curve versus glucose concentration constructed according to the colorimetric method of the invention., For sample 7 of Table III.
  • the colorimetric system for measuring the sugar content in wines and juices in general, in the field or on a production line consists of the components shown in Figure 1. That is, a closed container containing inside: one or more wells (A), preferably porcelain wells, for depositing liquid samples; an internal lighting medium (B); and an image capture means, preferably, a digital camera; and an analytical center comprising a data processing medium, preferably a computer (C), to analyze each color according to Red (R), green (G) and blue (B) contributions, calculating the average of the blue contributions for each It shows, and plotting said average against the corresponding sugar concentration, and with it, construct a corresponding calibration line (D) that allows to determine the sugar content in the samples.
  • A wells
  • B internal lighting medium
  • an image capture means preferably, a digital camera
  • an analytical center comprising a data processing medium, preferably a computer (C), to analyze each color according to Red (R), green (G) and blue (B) contributions, calculating the average of the blue contributions for
  • the colorimetric method for measuring the sugar content in wines and juices in general, in the field or in a production line comprises: providing liquid samples, in one or more wells, inside a container; illuminate the samples with a lighting medium located inside the container; and capture images that are then, remotely or directly, taken to an analytical center, where they are processed in a data processing medium, preferably a computer (C); analyzing for each color, the RGB contributions, and calculating the average of the contributions of blue for each sample, plotting said average against the corresponding sugar concentration, to construct relevant calibration line (D) that allows to determine the sugar content in the samples.
  • a data processing medium preferably a computer (C)
  • the sugar concentration is measured by the Dubois method, using the reddish color that develops by reaction between the sugars present and phenol, in the presence of sulfuric acid.
  • Dubois reagent employs one part of 5% aqueous phenol solution and five parts concentrated sulfuric acid. To this reagent, a part of a glucose solution is added, in a concentration range of 0-1%. For the initial calibration of the system a minimum of five liquid samples, in this concentration range. For sugar measurements in liquid samples with unknown sugar content, the samples are diluted so as to obtain sugar concentrations in a similar range (0-1%).
  • the system uses a container inside which has an internal lighting medium and an image capture medium, preferably a digital camera, which takes images of liquid samples or solutions deposited in porcelain wells inside the container, see Figure 2.
  • an image capture medium preferably a digital camera
  • Each image is transmitted, directly or remotely, by means of image capture to an analytical center where a data processor, preferably a computer, analyzes with the support of a software, preferably the Color Mania program, available on the network , quantitatively characterizing the color according to its RGB components (Red ("Red"), green ("Green”) and blue (“Blue”)).
  • a data processor preferably a computer
  • RGB components Red
  • Green Green
  • Blue blue
  • An operator selects 5 to 10 points in the image, obtaining for each of the points a value of R (red), G (green) and B (blue), see Figure 3.
  • the values of G are averaged, obtaining for each image an average value that is associated with the concentration of sugar in the liquid sample.
  • a graph of values of B versus sugar concentration, in a concentration range of 0-1%, allows the construction of a system calibration line that allows in turn to determine unknown values of sugar concentration by interpolation, see Figure 4 .
  • Example 1 of application of the invention - Analysis of a wine sample yielded an average value of 6.67 g / L of sugar by the Dubois method, on average of five measures (see Table 1), using a calibration based on a UV-Visible spectrophotometer. The same analysis, using a calibration based on the colorimetric method of the invention yielded an average value of 7.08 g / L, see Table 2. a) Construction of a calibration curve for glucose, based on measurements made with a UV spectrometer - visible. (sample 7)
  • solution A 100 mL of a 5% w / v phenol solution (solution A) was prepared.
  • Six solutions of 100 mL of glucose in water were prepared, with concentrations of 1.09% (solution 1); 2.18% (solution 2); 4.36% (solution 3); 6.54% (solution 4); 8.72% (solution 5) and 10.9% w / v (solution 6).
  • solution A 10 mL of a 5% w / v phenol solution
  • solution A 10 mL of a 5% w / v phenol solution
  • Six 10 mL solutions of glucose in water were prepared, with concentrations of 1.09% (solution 1); 2.18% (solution 2); 4.36% (solution 3); 6.54% (solution 4); 8.72% (solution 5) and 10.9% w / v (solution 6).
  • 0.5 mL of solution A was mixed with 2.5 mL of concentrated sulfuric acid and 0.5 mL of solutions 1-6 was added to each tube.
  • Table 1 Sugar concentration values in an unknown wine sample (sample 7 of table III), obtained in quintuplicate, with a UV-visible spectrophotometer
  • Table 2 Concentration values of sugars in an unknown sample of wine (sample 7 of table III), obtained in decuplicate, with the colorimetric method of the invention.
  • Example 2 of application of the invention The procedure described in example 1 of application was used for the analysis of 13 wine samples.
  • Table 3 the values obtained with the colorimetric method of the invention are compared with the concentration values obtained using the same Dubois test, but using a conventional UV-visible spectrophotometer.
  • Table 3 Concentration values of total sugars in 13 wine samples, obtained through the Dubois colorimetric method, using the system described in this invention, and a conventional UV-Visible spectrophotometer.

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  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Spectrometry And Color Measurement (AREA)

Abstract

L'invention concerne un système et un procédé colorimétrique pour mesurer la teneur en sucres des vins et des jus en général, dans un champ ou sur une ligne de production, les données colorimétriques étant générées à partir d'images qui ont été préalablement capturées et traitées, par envoi des données d'images, en direct ou à distance à une centrale analytique pour déterminer la teneur en sucre de l'échantillon de vin ou de jus. Le système et le procédé ont une précision équivalente à celle d'une analyse utilisant un spectromètre à ultraviolet visible (UV). 10
PCT/CL2015/050019 2014-06-19 2015-06-19 Système et procédé colorimétrique pour mesurer la teneur en sucres des vins et des jus Ceased WO2015192261A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CL2014001629A CL2014001629A1 (es) 2014-06-19 2014-06-19 Sistema y metodo colorimetrico para medir el contenido de azucares en vinos y jugos en general, en el campo o en una linea de produccion donde datos colorimetricos son generados a partir de imagenes que han sido previamente capturadas y procesadas, enviando los datos de imagen, en forma directa o remota a una central analitica para determinar el contenido de azucar en la muestra de vino o jugo.
CL1629-2014 2014-06-19

Publications (1)

Publication Number Publication Date
WO2015192261A1 true WO2015192261A1 (fr) 2015-12-23

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CL (1) CL2014001629A1 (fr)
WO (1) WO2015192261A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018197596A1 (fr) * 2017-04-28 2018-11-01 Koninklijke Philips N.V. Dispositif et procédé de détermination d'informations de teneur en sucre pour un article alimentaire

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT201700096325A1 (it) 2017-08-25 2019-02-25 Della Toffola Spa Metodo ed impianto di controllo per vinificazione

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090325631A1 (en) * 2008-06-30 2009-12-31 Nokia Corporation Color detection with a mobile device
US20110063433A1 (en) * 2009-09-11 2011-03-17 Thonhauser Gmbh Absorbance measurements using portable electronic devices with built-in camera
CL2014001150A1 (es) * 2014-04-30 2014-11-07 Lab4U Spa Sistema y metodo donde el sistema particularmente es un dispositivo movil, para la determinacion de la concentracion de solutos en una muestra liquida coloreada, el cual se basa en la obtencion de imagenes de la muestra y de patrones de concentracion conocidos del soluto, y la determinacion de la concentracion de la muestra sobre la base del procesamiento de esas imagenes y los valores de color que las mismas proporcionan

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090325631A1 (en) * 2008-06-30 2009-12-31 Nokia Corporation Color detection with a mobile device
US20110063433A1 (en) * 2009-09-11 2011-03-17 Thonhauser Gmbh Absorbance measurements using portable electronic devices with built-in camera
CL2014001150A1 (es) * 2014-04-30 2014-11-07 Lab4U Spa Sistema y metodo donde el sistema particularmente es un dispositivo movil, para la determinacion de la concentracion de solutos en una muestra liquida coloreada, el cual se basa en la obtencion de imagenes de la muestra y de patrones de concentracion conocidos del soluto, y la determinacion de la concentracion de la muestra sobre la base del procesamiento de esas imagenes y los valores de color que las mismas proporcionan

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DUBOIS M. ET AL.: "Colorimetric Method for Determination of Sugars and Related Substances.", ANAL. CHEM., vol. 28, no. 3, 1956, pages 350 - 356, XP000918426, doi:10.1021/ac60111a017 *
MATSUHIRO B. ET AL.: "Determination of low molecular weight carbohydrates in cabernet Sauvignon red wines.", J. CHIL. CHEM. SOC., vol. 54, no. 4, 2009, pages 405 - 407, XP055245865, Retrieved from the Internet <URL:http://dx.doi.org/10.4067/S0717-9707200900040001> *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018197596A1 (fr) * 2017-04-28 2018-11-01 Koninklijke Philips N.V. Dispositif et procédé de détermination d'informations de teneur en sucre pour un article alimentaire

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