WO2015190116A1 - Microalgae culture method, microalgae, and fat/oil production method - Google Patents
Microalgae culture method, microalgae, and fat/oil production method Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- the present disclosure relates to a method for culturing microalgae, a microalgae, and a method for producing fats and oils.
- Non-Patent Document 1 discloses that Chlamydomonas accumulates triacylglycerol when a stress that inhibits growth and proliferation is applied.
- the inventors of the present application have found the following. It is required to further improve the productivity when producing fats and oils using microalgae.
- This disclosure is intended to provide a method for culturing microalgae, a microalgae, and a method for producing fats and oils that can improve the productivity of fats and oils.
- a method for culturing microalgae is a method for culturing microalgae using a culture medium, wherein the molar concentration of phosphorus with respect to the molar concentration of nitrogen in the culture medium at the start of culture. Is within the range of 0.1 to 0.6.
- microalgae cultured by the microalgae culture method are provided.
- a method for producing fats and oils by extracting the fats and oils from the microalgae cultured by the microalgae culture method.
- the productivity of fats and oils can be improved.
- FIG. 1 is a diagram showing dry weight DW and fat content R for each medium
- FIG. 2 is a diagram showing the amount of oil T for each medium.
- FIG. 3 is a diagram showing the relationship between the culture time and the ratio X
- FIG. 4 is a diagram showing the composition of seven types of media
- FIG. 5 is a diagram showing the major axis, minor axis, and volume of algal bodies in the culture media B4, B5, and B6.
- the medium used in the present embodiment is not particularly limited and can be appropriately selected.
- Examples of the medium include AF6 medium.
- a phosphorus source (a source of phosphorus), a nitrogen source (a source of nitrogen), and the like can be added to the medium.
- Examples of the phosphorus source include monoammonium phosphate, diammonium phosphate, monocalcium phosphate, dicalcium phosphate, tricalcium phosphate, magnesium phosphate, and the like.
- the nitrogen source include urea, ammonium sulfate, ammonium nitrate, sodium nitrate, calcium nitrate, and potassium nitrate.
- the culture start time is a time when all the culture media in the culture vessel are made new and the culture is started with the new culture media.
- the culture start time is a time point when a part of the medium in the culture container is replaced with a new medium and the culture is started with the replaced medium.
- the culture start time is a time point when a culture medium is further added to the culture medium in the culture vessel and the culture is started with the added culture medium.
- the culture start time is a time point when a part of the components are added to the medium in the culture container and the culture is started with the added medium.
- a nitrogen source, a phosphorus source, and a medium containing them can be prevented from being added after the start of culture until the microalgae are collected.
- the amount of fats and oils obtained from microalgae can be further increased.
- the microalgae to be cultured is not particularly limited and can be appropriately selected from those producing oils and fats.
- Examples of the microalgae include green algal plants of the Trevoxia algae network including Chlorella, Trevoxia and the like.
- the molar concentration of nitrogen in the medium means the molar concentration of nitrogen atoms in the medium (excluding microalgae).
- the molar concentration of phosphorus in the medium means the molar concentration of phosphorus atoms in the medium (excluding microalgae).
- the ratio of the molar concentration of phosphorus to the molar concentration of nitrogen can be defined as follows. Let CN be the molar concentration of nitrogen in the medium. The molar concentration of phosphorus in the medium is CP. The ratio of the molar concentration of phosphorus to the molar concentration of nitrogen is expressed as CP / CN.
- the ratio of the molar concentration of phosphorus to the molar concentration of nitrogen is in the range of 0.1 to 0.6, preferably in the range of 0.2 to 0.5, preferably 0.3 to 0.4. More preferably within the range. By being within these ranges, the amount of fats and oils obtained from microalgae can be increased.
- the culture was performed under the conditions that the medium was irradiated with light (light intensity: 300 ⁇ mol / m 2 / s) using a straight tube LED, the temperature was 25 ° C., and a gas with a carbon dioxide concentration of 1% was aerated.
- the pH of the medium was adjusted to 3.5 using sulfuric acid before planting the microalgae and not adjusted thereafter.
- the culture was performed until 240 hours had passed since the start of the culture.
- algal bodies hereinafter simply referred to as algal bodies
- fats and oils were taken out from the collected algal bodies by a known method. Therefore, the fats and oils were able to be manufactured according to the above process.
- the culture medium seven types of B1 to B7 having the composition shown in FIG. 4 were prepared, and each was cultured by the above method.
- the composition of the medium shown in FIG. 4 is the composition at the start of culture.
- Media B1 to B7 commonly contain the following components.
- the culture media B1 to B7 each contain a potassium source, a calcium source, a magnesium source, a sodium source, and a chelate metal salt.
- N means the weight of nitrogen atoms in the nitrogen source.
- P means the weight of the phosphorus atom in the phosphorus source.
- K means the weight of potassium atom in the potassium source.
- Ca means the weight of calcium atoms in the calcium source.
- Mg means the weight of magnesium atom in the magnesium source.
- Na means the weight of sodium atom in the sodium source.
- CP / CN means the ratio of the molar concentration of phosphorus to the molar concentration of nitrogen in the medium at the start of culture.
- the dry weight DW, the fat content R, and the fat content T were larger than when the culture media B6 and B7 were used.
- the culture media B2 to B4 were used, the dry weight DW, the fat content R, and the fat content T were larger.
- medium B3 was used, dry weight DW, fat content R, and fat amount T were particularly large.
- the reason why the fat and oil content R and the fat and oil amount T increase when the culture media B1 to B5 are used can be estimated as follows. Since mediums B1 to B5 have small CP / CN, when these mediums are used, phosphorus is deficient in microalgae and cell division is suppressed. This is supported by an increase in the size per cell when a medium having a small CP / CN is used, as will be described later in (3). By suppressing cell division, the energy required for cell division can be used for CO 2 fixation by photosynthesis, and as a result, the fat content R and the fat amount T increase.
- the microalgae to be cultured may be those other than Pseudococomixa KJ strain, and can be appropriately selected from those producing oils and fats.
- composition of the medium used for the culture can be appropriately set under conditions where the CP / CN ratio is within a predetermined range.
- the culture method may be a method in which the culture is started after all the culture medium is new, or after the end of the previous culture, replacement of a part of the culture medium, addition of the culture medium, addition of the components of the culture medium, etc. Then, a method of starting the next culture may be used.
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Abstract
Description
本出願は、２０１４年６月１３日に出願された日本国特許出願２０１４－１２２４３８号、に基づくものであり、ここにその記載内容を参照により援用する。 This application is based on Japanese Patent Application No. 2014-122438 filed on June 13, 2014, the contents of which are incorporated herein by reference.
本開示は、微細藻類の培養方法、微細藻類、及び油脂の製造方法に関する。 The present disclosure relates to a method for culturing microalgae, a microalgae, and a method for producing fats and oils.
近年、微細藻類が生産する油脂を燃料等へ利活用することが注目されている。非特許文献１には、成長や増殖を阻害するようなストレスを与えると、クラミドモナスがトリアシルグリセロールを蓄積することが開示されている。
In recent years, the use of oils and fats produced by microalgae as fuel has attracted attention. Non-Patent
本願発明者らは、下記を見出した。微細藻類を用いて油脂を生産するときの生産性をさらに向上させることが求められている。 The inventors of the present application have found the following. It is required to further improve the productivity when producing fats and oils using microalgae.
本開示は、油脂の生産性を向上させることができる微細藻類の培養方法、微細藻類、及び油脂の製造方法を提供することを目的とする。 This disclosure is intended to provide a method for culturing microalgae, a microalgae, and a method for producing fats and oils that can improve the productivity of fats and oils.
本開示の一態様によれば、微細藻類の培養方法は、培地を用いて微細藻類を培養する微細藻類の培養方法であって、培養開始時において、培地における窒素のモル濃度に対するリンのモル濃度の比率が、０．１～０．６の範囲内である。 According to one aspect of the present disclosure, a method for culturing microalgae is a method for culturing microalgae using a culture medium, wherein the molar concentration of phosphorus with respect to the molar concentration of nitrogen in the culture medium at the start of culture. Is within the range of 0.1 to 0.6.
本開示の別の一態様によれば、微細藻類の培養方法により培養された微細藻類が提供される。 According to another aspect of the present disclosure, microalgae cultured by the microalgae culture method are provided.
本開示の別の一態様によれば、微細藻類の培養方法により培養された微細藻類から、油脂を取り出す油脂の製造方法が提供される。 According to another aspect of the present disclosure, there is provided a method for producing fats and oils by extracting the fats and oils from the microalgae cultured by the microalgae culture method.
本開示の微細藻類の培養方法、微細藻類、及び油脂の製造方法によれば、油脂の生産性を向上させることができる。 According to the method for culturing microalgae, the microalgae, and the method for producing fats and oils of the present disclosure, the productivity of fats and oils can be improved.
本開示についての上記および他の目的、特徴や利点は、添付の図面を参照した下記の詳細な説明から、より明確になる。添付図面において
以下、実施形態について説明する。本実施形態で使用する培地は特に限定されず、適宜選択できる。培地としては、例えば、AF6培地等が挙げられる。培地には、リン源（リンの供給源）、窒素源（窒素の供給源）等を添加することができる。リン源としては、例えば、リン酸一アンモニウム、リン酸二アンモニウム、リン酸一カルシウム、リン酸二カルシウム、リン酸三カルシウム、リン酸マグネシウム等が挙げられる。窒素源としては、例えば、尿素、硫酸アンモニウム、硝酸アンモニウム、硝酸ナトリウム、硝酸カルシウム、硝酸カリウム等が挙げられる。 Hereinafter, embodiments will be described. The medium used in the present embodiment is not particularly limited and can be appropriately selected. Examples of the medium include AF6 medium. A phosphorus source (a source of phosphorus), a nitrogen source (a source of nitrogen), and the like can be added to the medium. Examples of the phosphorus source include monoammonium phosphate, diammonium phosphate, monocalcium phosphate, dicalcium phosphate, tricalcium phosphate, magnesium phosphate, and the like. Examples of the nitrogen source include urea, ammonium sulfate, ammonium nitrate, sodium nitrate, calcium nitrate, and potassium nitrate.
培養開始時とは、例えば、以下の時点が該当する。培養開始時とは、培養容器内の培地を全て新規なものとし、新規な培地で培養を開始する時点である。培養開始時とは、培養容器内の培地における一部を新規な培地に置換し、置換後の培地で培養を開始する時点である。培養開始時とは、培養容器内の培地にさらに培地を追加し、追加後の培地で培養を開始する時点である。培養開始時とは、培養容器内の培地に一部の成分を追加し、追加後の培地で培養を開始する時点である。 For example, the following time points correspond to the start of culture. The culture start time is a time when all the culture media in the culture vessel are made new and the culture is started with the new culture media. The culture start time is a time point when a part of the medium in the culture container is replaced with a new medium and the culture is started with the replaced medium. The culture start time is a time point when a culture medium is further added to the culture medium in the culture vessel and the culture is started with the added culture medium. The culture start time is a time point when a part of the components are added to the medium in the culture container and the culture is started with the added medium.
培養開始時以降、微細藻類を回収するまで、例えば、窒素源、リン源、それらを含む培地の追加を行わないようにすることができる。この場合、微細藻類から得られる油脂の量を一層多くすることができる。 For example, a nitrogen source, a phosphorus source, and a medium containing them can be prevented from being added after the start of culture until the microalgae are collected. In this case, the amount of fats and oils obtained from microalgae can be further increased.
培養する微細藻類は特に限定されず、油脂等を生産するものから、適宜選択できる。微細藻類としては、例えば、クロレラ目、トレボウキシア目等を含むトレボウキシア藻網の緑藻植物等が挙げられる。 The microalgae to be cultured is not particularly limited and can be appropriately selected from those producing oils and fats. Examples of the microalgae include green algal plants of the Trevoxia algae network including Chlorella, Trevoxia and the like.
培地における窒素のモル濃度とは、培地（微細藻類は除く）における窒素原子のモル濃度を意味する。また、培地におけるリンのモル濃度とは、培地（微細藻類は除く）におけるリン原子のモル濃度を意味する。 The molar concentration of nitrogen in the medium means the molar concentration of nitrogen atoms in the medium (excluding microalgae). The molar concentration of phosphorus in the medium means the molar concentration of phosphorus atoms in the medium (excluding microalgae).
窒素のモル濃度に対するリンのモル濃度の比率とは、以下のように定義できる。培地における窒素のモル濃度をＣＮとする。培地におけるリンのモル濃度をＣＰとする。窒素のモル濃度に対するリンのモル濃度の比率は、ＣＰ／ＣＮで表される。 The ratio of the molar concentration of phosphorus to the molar concentration of nitrogen can be defined as follows. Let CN be the molar concentration of nitrogen in the medium. The molar concentration of phosphorus in the medium is CP. The ratio of the molar concentration of phosphorus to the molar concentration of nitrogen is expressed as CP / CN.
窒素のモル濃度に対するリンのモル濃度の比率は、０．１～０．６の範囲内であり、０．２～０．５の範囲内であることが好ましく、０．３～０．４の範囲内であることが一層好ましい。これらの範囲内であることにより、微細藻類から得られる油脂の量を多くすることができる。 The ratio of the molar concentration of phosphorus to the molar concentration of nitrogen is in the range of 0.1 to 0.6, preferably in the range of 0.2 to 0.5, preferably 0.3 to 0.4. More preferably within the range. By being within these ranges, the amount of fats and oils obtained from microalgae can be increased.
微細藻類から、油脂を取り出す方法としては、公知の方法を適宜用いることができる。
（実施例）
（培養方法）
５００ｍｌ扁平フラスコに培地を導入した。その培地に、微細藻類の一例であるシュードココミクサ（Pseudococcomyxa）ＫＪ株を、０．０２ｇ／ｌとなるように植株した。シュードココミクサＫＪ株は、２０１３年６月４日付で独立行政法人製品評価技術基盤機構 特許生物寄託センター（ＮＩＴＥ－ＩＰＯＤ）（千葉県木更津市かずさ鎌足２－５－８ １２０号室）に受託番号ＦＥＲＭ Ｐ－２２２５４として寄託され、その後、ブダペスト条約の規定下で、受託番号ＦＥＲＭ ＡＢＰ－２２２５４として国際寄託に移管されている。
As a method for extracting oils and fats from microalgae, known methods can be used as appropriate.
(Example)
(Culture method)
The medium was introduced into a 500 ml flat flask. In the medium, Pseudococcomyxa KJ strain, which is an example of microalgae, was planted so as to be 0.02 g / l. Pseudocomicus KJ Co., Ltd. was accepted on June 4, 2013 by the National Institute of Technology and Evaluation Biological Depositary (NITE-IPOD) (Kazusa-Kamazu 2-5-8, 120, Kisarazu, Chiba). Deposited as FERM P-22254 and subsequently transferred to the International Deposit as deposit number FERM ABP-22254 under the provisions of the Budapest Treaty.
直管型LEDを用いて培地に光（光強度：300μmol/m2/s）を照射し、温度を２５℃とし、二酸化炭素濃度１％のガスを通気するという条件で培養を行った。培地のｐＨは、微細藻類の植株前に硫酸を用いて３．５に調整し、その後は調整しなかった。培養は、培養開始から240時間が経過するまで行った。培養終了後、培地からシュードココミクサＫＪ株の藻体（以下、単に藻体とする）を回収した。さらに、回収した藻体から、周知の方法で油脂を取り出した。よって、以上の工程により、油脂を製造することができた。 The culture was performed under the conditions that the medium was irradiated with light (light intensity: 300 μmol / m 2 / s) using a straight tube LED, the temperature was 25 ° C., and a gas with a carbon dioxide concentration of 1% was aerated. The pH of the medium was adjusted to 3.5 using sulfuric acid before planting the microalgae and not adjusted thereafter. The culture was performed until 240 hours had passed since the start of the culture. After completion of the culture, algal bodies (hereinafter simply referred to as algal bodies) of Pseudococomixa KJ strain were collected from the medium. Furthermore, fats and oils were taken out from the collected algal bodies by a known method. Therefore, the fats and oils were able to be manufactured according to the above process.
培地としては、図４に示す組成を有するＢ１～Ｂ７の７種類を用意し、それぞれにおいて、上記の方法で培養を行った。図４に示す培地の組成は、培養開始時における組成である。 As the culture medium, seven types of B1 to B7 having the composition shown in FIG. 4 were prepared, and each was cultured by the above method. The composition of the medium shown in FIG. 4 is the composition at the start of culture.
培地Ｂ１～Ｂ７は共通して、以下の成分を含む。 Media B1 to B7 commonly contain the following components.
尿素（窒素源）
リン酸一アンモニウム（リン源）
また、培地Ｂ１～Ｂ７は、それぞれ、カリウム源、カルシウム源、マグネシウム源、ナトリウム源、及びキレート金属塩を含む。
Urea (nitrogen source)
Monoammonium phosphate (phosphorus source)
The culture media B1 to B7 each contain a potassium source, a calcium source, a magnesium source, a sodium source, and a chelate metal salt.
図４において「Ｎ」は、窒素源のうち、窒素原子の分の重量を意味する。「Ｐ」は、リン源のうち、リン原子の分の重量を意味する。「Ｋ」は、カリウム源のうち、カリウム原子の重量を意味する。「Ｃａ」は、カルシウム源のうち、カルシウム原子の重量を意味する。「Ｍｇ」は、マグネシウム源のうち、マグネシウム原子の重量を意味する。「Ｎａ」は、ナトリウム源のうち、ナトリウム原子の重量を意味する。 In FIG. 4, “N” means the weight of nitrogen atoms in the nitrogen source. “P” means the weight of the phosphorus atom in the phosphorus source. “K” means the weight of potassium atom in the potassium source. “Ca” means the weight of calcium atoms in the calcium source. “Mg” means the weight of magnesium atom in the magnesium source. “Na” means the weight of sodium atom in the sodium source.
また、図４において、「ＣＰ／ＣＮ」は、培養開始時における、倍地中での窒素のモル濃度に対するリンのモル濃度の比率を意味する。
（評価）
（１）単位体積の培地から回収した藻体の乾燥重量ＤＷ（ｇ／Ｌ）を算出した。また、乾燥した状態にある藻体の全重量に対し油脂が占める比率（以下、油脂含量Ｒ（重量％）とする）を算出した。また、乾燥重量ＤＷと油脂含量Ｒとを乗算することで、単位体積の培地から得られる油脂量Ｔを算出した。これらの乾燥重量ＤＷ、油脂含量Ｒ、及び油脂量Ｔは、培地Ｂ１～Ｂ７のそれぞれについて算出した。乾燥重量ＤＷ、及び油脂含量Ｒの算出結果を図１に示す。また、油脂量Ｔの算出結果を図２に示す。
In FIG. 4, “CP / CN” means the ratio of the molar concentration of phosphorus to the molar concentration of nitrogen in the medium at the start of culture.
(Evaluation)
(1) The dry weight DW (g / L) of algal bodies recovered from a unit volume of medium was calculated. Moreover, the ratio (henceforth fat content R (weight%)) which fats and oils occupy with respect to the total weight of the algal body in the dried state was computed. Further, by multiplying the dry weight DW by the fat content R, the fat amount T obtained from the unit volume of the medium was calculated. The dry weight DW, fat content R, and fat amount T were calculated for each of the media B1 to B7. The calculation results of the dry weight DW and the fat content R are shown in FIG. Moreover, the calculation result of the oil-fat amount T is shown in FIG.
図１、図２に示されているように、培地Ｂ１～Ｂ５を用いた場合、培地Ｂ６、Ｂ７を用いた場合よりも、乾燥重量ＤＷ、油脂含量Ｒ、及び油脂量Ｔが大きかった。また、培地Ｂ２～Ｂ４を用いた場合は、乾燥重量ＤＷ、油脂含量Ｒ、及び油脂量Ｔが一層大きかった。また、培地Ｂ３を用いた場合は、乾燥重量ＤＷ、油脂含量Ｒ、及び油脂量Ｔが特に大きかった。 As shown in FIGS. 1 and 2, when the culture media B1 to B5 were used, the dry weight DW, the fat content R, and the fat content T were larger than when the culture media B6 and B7 were used. In addition, when the culture media B2 to B4 were used, the dry weight DW, the fat content R, and the fat content T were larger. When medium B3 was used, dry weight DW, fat content R, and fat amount T were particularly large.
培地Ｂ１～Ｂ５を用いた場合に、油脂含量Ｒ、及び油脂量Ｔが大きくなる理由は以下のように推測できる。培地Ｂ１～Ｂ５では、ＣＰ／ＣＮが小さいので、これらの培地を用いると、微細藻類においてリンが欠乏し、細胞分裂が抑制される。そのことは、後述する（３）で示すように、ＣＰ／ＣＮが小さい培地を用いると細胞１個当りの大きさが大きくなることにより裏付けられる。細胞分裂が抑制されることにより、細胞分裂に要していたエネルギーを光合成によるＣＯ２固定に利用でき、結果として、油脂含量Ｒ、及び油脂量Ｔが大きくなる。 The reason why the fat and oil content R and the fat and oil amount T increase when the culture media B1 to B5 are used can be estimated as follows. Since mediums B1 to B5 have small CP / CN, when these mediums are used, phosphorus is deficient in microalgae and cell division is suppressed. This is supported by an increase in the size per cell when a medium having a small CP / CN is used, as will be described later in (3). By suppressing cell division, the energy required for cell division can be used for CO 2 fixation by photosynthesis, and as a result, the fat content R and the fat amount T increase.
（２）培養終了後、回収した藻体の写真を撮影した。藻体は楕円形形状を有していた。撮影した写真において、藻体の長径及び短径を計測し、それらに基づき藻体の細胞１個当りの体積を算出した。その結果を図５に示す。 (2) After culturing, a photograph of the collected alga bodies was taken. The algal bodies had an oval shape. In the photograph taken, the major axis and minor axis of the alga body were measured, and the volume per cell of the alga body was calculated based on them. The result is shown in FIG.
図５に示されているように、培地Ｂ４、Ｂ５を用いた場合は、培地Ｂ６を用いた場合よりも、長径及び短径が長く、細胞１個当りの体積が大きかった。 As shown in FIG. 5, when mediums B4 and B5 were used, the major axis and the minor axis were longer and the volume per cell was larger than when medium B6 was used.
（３）培養中、培地の一部を定期的に取出し、藻体の全乾燥重量に対する、藻体に含まれるリンの重量の比率Ｘを算出した。その結果を図３に示す。図３に示されているように、培地Ｂ１～Ｂ５、Ｂ７を用いた場合、培地Ｂ６を用いた場合よりも、同じ培養時間で比べて、比率Ｘの値が低かった。また、培地Ｂ１～Ｂ５、Ｂ７を用いた場合、培地Ｂ６を用いた場合よりも、比率Ｘが一定の値に達するまでの培養時間が短かった。 (3) During the culture, a part of the medium was periodically taken out, and the ratio X of the weight of phosphorus contained in the alga body to the total dry weight of the alga body was calculated. The result is shown in FIG. As shown in FIG. 3, when mediums B1 to B5 and B7 were used, the value of ratio X was lower than that when medium B6 was used, compared with the same culture time. In addition, when the culture media B1 to B5 and B7 were used, the culture time until the ratio X reached a certain value was shorter than when the culture media B6 was used.
以上、実施形態について説明したが、本開示は上記実施形態に限定されることなく、種々の形態を採り得る。 As mentioned above, although embodiment was described, this indication can take various forms, without being limited to the above-mentioned embodiment.
培養する微細藻類はシュードココミクサＫＪ株以外のものであってもよく、油脂を生産するものの中から、適宜選択できる。 The microalgae to be cultured may be those other than Pseudococomixa KJ strain, and can be appropriately selected from those producing oils and fats.
培養に用いる培地の組成は、ＣＰ／ＣＮの比率が所定の範囲内である条件下において、適宜設定できる。 The composition of the medium used for the culture can be appropriately set under conditions where the CP / CN ratio is within a predetermined range.
培養方法は、培地を全て新規なものとしてから培養を開始する方法であってもよいし、前回の培養の終了後、培地の一部の置換、培地の追加、培地の成分の追加等をしてから、次の培養を開始する方法であってもよい。 The culture method may be a method in which the culture is started after all the culture medium is new, or after the end of the previous culture, replacement of a part of the culture medium, addition of the culture medium, addition of the components of the culture medium, etc. Then, a method of starting the next culture may be used.
以上、微細藻類の培養方法、微細藻類、及び油脂の製造方法の実施形態、構成、態様を例示したが、微細藻類の培養方法、微細藻類、及び油脂の製造方法に係わる実施形態、構成、態様は、上述した各実施形態、各構成、各態様に限定されるものではない。例えば、異なる実施形態、構成、態様にそれぞれ開示された技術的部を適宜組み合わせて得られる実施形態、構成、態様についても微細藻類の培養方法、微細藻類、及び油脂の製造方法に係わる実施形態、構成、態様の範囲に含まれる。 The embodiments, configurations, and aspects of the method for culturing microalgae, the microalgae, and the fats and oils have been exemplified above. Is not limited to the above-described embodiments, configurations, and aspects. For example, embodiments relating to microalgae culture method, microalgae, and oil and fat production method for embodiments, configurations, and aspects obtained by appropriately combining technical parts disclosed in different embodiments, configurations, and aspects, It is contained in the range of a structure and an aspect.
Claims (7)
培養開始時において、前記培地における窒素のモル濃度に対するリンのモル濃度の比率が、０．１～０．６の範囲内である微細藻類の培養方法。 A method for cultivating microalgae using a medium to culture microalgae,
A method for culturing microalgae, wherein the ratio of the molar concentration of phosphorus to the molar concentration of nitrogen in the medium is within the range of 0.1 to 0.6 at the start of culture.
培養開始時において、前記比率が０．２～０．５の範囲内である微細藻類の培養方法。 A method for culturing microalgae according to claim 1,
A method for culturing microalgae, wherein the ratio is in the range of 0.2 to 0.5 at the start of culture.
培養開始時において、前記比率が０．３～０．４の範囲内である微細藻類の培養方法。 A method for culturing microalgae according to claim 1,
A method for culturing microalgae, wherein the ratio is in the range of 0.3 to 0.4 at the start of culturing.
培養開始時において、前記培地における窒素のモル濃度に対するリンのモル濃度の比率が、０．１５～０．５７の範囲内である微細藻類の培養方法。 A method for culturing microalgae according to claim 1,
A method for culturing microalgae, wherein the ratio of the molar concentration of phosphorus to the molar concentration of nitrogen in the medium is within the range of 0.15 to 0.57 at the start of culture.
培養開始時において、前記培地における窒素のモル濃度に対するリンのモル濃度の比率が、０．１５、０．２５、０．３５、０．４５、０．５７のいずれかである微細藻類の培養方法。 A method for culturing microalgae according to claim 6,
The method for culturing microalgae, wherein the ratio of the molar concentration of phosphorus to the molar concentration of nitrogen in the medium is 0.15, 0.25, 0.35, 0.45, or 0.57 at the start of the culture .
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