[go: up one dir, main page]

WO2015152458A1 - Multi-comb - Google Patents

Multi-comb Download PDF

Info

Publication number
WO2015152458A1
WO2015152458A1 PCT/KR2014/004066 KR2014004066W WO2015152458A1 WO 2015152458 A1 WO2015152458 A1 WO 2015152458A1 KR 2014004066 W KR2014004066 W KR 2014004066W WO 2015152458 A1 WO2015152458 A1 WO 2015152458A1
Authority
WO
WIPO (PCT)
Prior art keywords
plate
body portion
comb
present
shape
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2014/004066
Other languages
French (fr)
Korean (ko)
Inventor
고창욱
김천기
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bionlifescience Inc
Original Assignee
Bionlifescience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionlifescience Inc filed Critical Bionlifescience Inc
Publication of WO2015152458A1 publication Critical patent/WO2015152458A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44743Introducing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers

Definitions

  • the present invention relates to a multicom, and more particularly to a multicom that can be used in various forms of a plate set that is a component of an electrophoretic device.
  • Electrophoretic apparatuses are widely used in proteins and DNA fractions in laboratories. When a DC voltage is applied to charged particles in an electrolyte, positively charged particles move toward the negative electrode and negatively charged particles move toward the positive electrode. will be.
  • gel electrophoresis is a general term for electrophoresis using gel as a support, and is mainly used in vertical electrophoresis apparatus.
  • FIG. 1 shows one embodiment of a vertical electrophoretic system.
  • the vertical electrophoretic system includes an electrophoretic apparatus 1, a casting stand 2, a casting frame 3, a packing pad 4, and spacers having predetermined dimensions at both ends.
  • an acrylamide gel plate (not shown), which is composed of a spacer plate 5, a short plate 6, and a comb 7, and is mounted on an electrophoretic device through such a system, first, a spacer plate and a short plate are prepared. To form a space between them, mount the plate set on the casting frame, and then fit the casting frame horizontally on the casting stand, but the casting stand where the lower part of the plate set is positioned to prevent the acrylamide gel from leaking under the plate set. Place the packing pad on the bottom.
  • the prepared separation gel is injected into the space between the plate sets using a dispenser to a certain height, and the separation gel is hardened for about 30 minutes, and then the prepared accumulation gel is injected into the extra space between the plate sets using the dispenser.
  • a comb is inserted from the upper direction to the lowering gel position between the plate sets to form a plurality of chambers.
  • the chamber is formed to contain the sample protein.
  • Combs used to form a plurality of chambers in such a conventional electrophoretic system system is customized to form according to the shape of the plate set of the brand-specific electrophoretic apparatus sold in the market, as shown in Figures 2 and 3 To be different. That is, the comb shape shown in FIGS. 2 (a) and 3 (a) is a plate set having a '-' shaped short plate shown in (a) of FIG. 2 due to the projections 7a formed up to both ends. Can only be used in form. In addition, the comb shape shown in Figs. 2 (b) and 3 (b) is of the plate set having the 'c' shaped short plate shown in Fig. 2 (b) due to the step 7b formed at both ends. Can only be used in form.
  • the present invention has been made to solve the above problems, and an object of the present invention is to provide a multicom which can be used in various forms of a plate set which is a component of an electrophoretic apparatus.
  • Leg portion consisting of a plurality of legs formed in the lower portion of the body portion
  • both end portions formed to be spaced apart from both ends of the body portion; characterized in that it comprises a.
  • the body portion is a rectangular plate shape having a thickness
  • the plurality of legs is characterized in that the odd number provided.
  • the body portion is further provided with a hole for preventing the shape distortion and slipping, the leg portion is characterized in that it further comprises auxiliary legs at both ends.
  • the protrusion is characterized in that the shape of the side cross-section is a "c" shape protruding from the front surface of the body portion.
  • the protrusion may further include a hole corresponding to the hole formed in the body portion.
  • FIG. 1 is a view schematically showing a configuration of an embodiment of a conventional electrophoretic device system
  • Figure 2 (a), (b) is a view showing a front view of the two types of combs used in the conventional electrophoretic device and a side view as seen from AA, BB,
  • FIG. 3 is a view illustrating a state in which each comb of FIG. 2 is coupled to each set of plates forming a set;
  • FIGS. 4A to 4E are bottom views (b), rear views (c) and (c) of the bottom view (a), the rear view (c) of (a) of the front view of one embodiment of the multi-com according to the present invention Top view (d) as seen from the right, the right side view as seen from B,
  • Figure 6 (a), (b) is a perspective view of another embodiment of a multicom according to the present invention.
  • FIG. 7 is a view illustrating a state in which the multicom of FIG. 6A is coupled to the plate set of FIG. 5.
  • FIG. 4 (a) to (e) is a front view, a back view, a top view, a bottom view, a right side view of an embodiment of a multi-com according to the present invention
  • Figure 5 shows two kinds of plate sets (short plate and spacer plate)
  • Figure 6 (a) is a perspective view of another embodiment of a multicom according to the present invention
  • Figure 7 is a view showing a state in which the multicomb of Figure 6 (a) is coupled to the plate set of Figure 5 .
  • the multi-com (100, 100 ') is a comb used in the electrophoretic system, the top of the accumulating gel injected into the extra space between the plate set from the top direction It is used to plug into, the body portion 10, the leg portion 20 consisting of a plurality of legs formed in the lower portion of the body and protrude integrally formed on the front surface of the body portion, both ends are spaced apart from both ends of the body portion by a certain distance It is configured to include a protrusion 30 formed.
  • the body portion 10 may have a rectangular plate shape having a predetermined thickness, and may further include a hole H that prevents form distortion and prevents slipping.
  • Leg portion 20 is formed in a plurality of legs in a predetermined interval at the lower portion of the body, is formed in accordance with the desired number of chambers.
  • the number of legs is typically provided in odd numbers to form an even number of chambers.
  • both ends of the leg portion may be further provided with a secondary leg (20a) is relatively short in length, narrow in width and facing outward a predetermined angle. The secondary leg facilitates this when the comb is placed in the extra space between the plate sets and prevents the accumulation gel filled in the extra space from drying out.
  • Protruding portion 30 is integrally formed on the front surface of the upper body 10, the both ends of the protrusion is formed to be spaced apart a predetermined distance (L) from both ends of the body, the shape of the side cross-section protrudes from the front of the body portion It may have a “c” shape and may have a rectangular hole H corresponding to the hole formed in the body portion.
  • the shape of the hole is not limited to the rectangular hole, and may be provided in various forms.
  • the protrusion facilitates plugging or unloading the multicomb in the extra space between the plate sets in the upward direction and downward, and prevents the multicom from falling into the extra space.
  • the protrusions are formed to be spaced apart from the both ends of the body portion by a predetermined distance (L), the plate set (50, 60) and 'with the'-'shaped short plate shown in (a), (b) of FIG. It can be used for both the plate set (50, 60 ') having a'-shaped short plate.
  • FIG. 5 (a) shows plate sets 50 and 60 with '-' shaped short plates 60
  • FIG. 5 (b) shows plates with 'c' shaped short plates 60 '.
  • Sets 50 and 60 ' are shown.
  • the upper inner edge of the spacer 50a attached to the spacer plate 50 of each plate set is formed to be inclined at a predetermined angle of a fine size. This is to facilitate the comb input.
  • FIG. 7 and 8 is a state diagram showing the fitting of the multicom according to the present invention to each plate set, as shown, the multicom according to the present invention can be used regardless of the shape of the plate set.
  • the present invention can be used simply and conveniently without being bound to various types of plate sets, and thus has industrial applicability for use in laboratory vertical protein electrophoresis systems.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)

Abstract

The present invention relates to a multi-comb and, more particularly, to a comb for use in an electrophoresis system, comprising: a body part; a leg part formed in the lower portion of the body part, the leg part consisting of a plurality of legs; and protrusions which are formed to protrude integrally from the front surface of the body part with both ends thereof being spaced apart from both ends of the body part. The present invention has the effect of being able to be used easily and conveniently regardless of the various forms of plate sets.

Description

멀티콤Multicom

본 발명은 멀티콤에 관한 것으로, 보다 상세하게는 전기영동장치의 구성부품인 플레이트세트의 다양한 형태에 사용될 수 있는 멀티콤에 관한 것이다.The present invention relates to a multicom, and more particularly to a multicom that can be used in various forms of a plate set that is a component of an electrophoretic device.

전기영동장치는 실험실에서 단백질이나 DNA 분획 등에 널리 사용되는 것으로, 이는 전해질 중에 존재하는 하전(荷電) 입자에 직류전압을 걸면 정의 하전 입자는 음극으로, 부의 하전 입자는 양극으로 향하여 이동하는 현상을 이용한 것이다.Electrophoretic apparatuses are widely used in proteins and DNA fractions in laboratories. When a DC voltage is applied to charged particles in an electrolyte, positively charged particles move toward the negative electrode and negatively charged particles move toward the positive electrode. will be.

전기영동의 종류 중 겔 전기영동은 지지체로서 겔을 사용하는 전기영동을 총칭하는 용어로서 수직형 전기영동장치에 주로 사용된다.Among the types of electrophoresis, gel electrophoresis is a general term for electrophoresis using gel as a support, and is mainly used in vertical electrophoresis apparatus.

도 1에는 수직형 전기영동장치 시스템의 일 실시예가 도시되어 있다. 1 shows one embodiment of a vertical electrophoretic system.

도 1에 도시된 바와 같이, 수직형 전기영동장치 시스템은 전기영동장치(1), 캐스팅스탠드(2), 캐스팅프레임(3), 패킹패드(4), 양측 단부에 소정치수의 스페이서를 구비한 스페이서플레이트(5)와 쇼트플레이트(6), 및 콤(7)으로 구성되며, 이와 같은 시스템을 통해 전기영동장치에 장착하는 아크릴아마이드 겔판(미도시)을 제조하기 위해서는, 우선 스페이서플레이트와 쇼트플레이트를 밀착시켜 사이 공간을 형성하고, 이 플레이트세트를 캐스팅프레임에 장착한 후, 캐스팅프레임을 캐스팅스탠드에 수평을 맞추어 끼우되 플레이트세트 하부로 아크릴아마이드 겔이 새어나오지 않도록 플레이트세트 하부가 위치하는 캐스팅스탠드 저면에 패킹패드를 배치한다. As shown in FIG. 1, the vertical electrophoretic system includes an electrophoretic apparatus 1, a casting stand 2, a casting frame 3, a packing pad 4, and spacers having predetermined dimensions at both ends. In order to manufacture an acrylamide gel plate (not shown), which is composed of a spacer plate 5, a short plate 6, and a comb 7, and is mounted on an electrophoretic device through such a system, first, a spacer plate and a short plate are prepared. To form a space between them, mount the plate set on the casting frame, and then fit the casting frame horizontally on the casting stand, but the casting stand where the lower part of the plate set is positioned to prevent the acrylamide gel from leaking under the plate set. Place the packing pad on the bottom.

그런 다음 준비된 분리겔을 분주기를 이용하여 플레이트세트 사이공간에 일정높이까지 주입하고 대략 30분정도 분리겔을 굳힌 후, 준비된 축적겔을 분주기를 이용하여 플레이트세트 사이의 여분공간에 주입한다. 다음, 플레이트세트 사이의 축적겔 위치에 상방향에서 하방향으로 콤을 꽂아 다수개의 약실을 형성한다. 약실은 샘플 단백질을 넣기 위해 형성하는 것으로, 겔판이 구비된 플레이트세트를 전기영동장치(1)에 배치하여 전기영동시키면, 샘플 단백질이 수직방향으로 분자량에 따라 분리된다.Then, the prepared separation gel is injected into the space between the plate sets using a dispenser to a certain height, and the separation gel is hardened for about 30 minutes, and then the prepared accumulation gel is injected into the extra space between the plate sets using the dispenser. Next, a comb is inserted from the upper direction to the lowering gel position between the plate sets to form a plurality of chambers. The chamber is formed to contain the sample protein. When the plate set provided with the gel plate is placed on the electrophoretic apparatus 1 and subjected to electrophoresis, the sample protein is separated according to the molecular weight in the vertical direction.

이와 같은 종래 전기영동장치 시스템에서 다수개의 약실을 형성하기 위해 사용되는 콤은 도 2 및 도 3에 도시된 바와 같이, 시중에서 판매되는 브랜드별 전기영동장치의 플레이트세트의 형태에 따라 맞춤식으로 그 형태를 달리한다. 즉, 도 2(a) 및 도 3(a)에 도시된 콤 형태는 양 단부까지 형성된 돌기(7a)로 인하여 도 2의 (a)에 도시된 'ㅡ'자형 쇼트플레이트를 구비한 플레이트세트의 형태에만 사용될 수 있다. 또한, 도 2(b) 및 도 3(b)에 도시된 콤 형태는 양 단부에 형성된 단차(7b)로 인하여 도 2의 (b)에 도시된 'ㄷ'자형 쇼트플레이트를 구비한 플레이트세트의 형태에만 사용될 수 있다. Combs used to form a plurality of chambers in such a conventional electrophoretic system system is customized to form according to the shape of the plate set of the brand-specific electrophoretic apparatus sold in the market, as shown in Figures 2 and 3 To be different. That is, the comb shape shown in FIGS. 2 (a) and 3 (a) is a plate set having a '-' shaped short plate shown in (a) of FIG. 2 due to the projections 7a formed up to both ends. Can only be used in form. In addition, the comb shape shown in Figs. 2 (b) and 3 (b) is of the plate set having the 'c' shaped short plate shown in Fig. 2 (b) due to the step 7b formed at both ends. Can only be used in form.

따라서, 실험자는 사용하는 전기영동장치마다 비싼 가격에 재구입하여야 하므로 경제적으로 낭비되는 문제도 있다.Therefore, the experimenter has to be repurchased at an expensive price for each electrophoretic device used, there is also a problem of economic waste.

본 발명은 상술한 문제점을 해결하기 위하여 안출된 것으로서, 본 발명의 목적은 전기영동장치의 구성부품인 플레이트세트의 다양한 형태에 사용될 수 있는 멀티콤을 제공하는 것이다.SUMMARY OF THE INVENTION The present invention has been made to solve the above problems, and an object of the present invention is to provide a multicom which can be used in various forms of a plate set which is a component of an electrophoretic apparatus.

상술한 과제를 해결하기 위하여, 본 발명에 따른 멀티콤은In order to solve the above problems, the multi-com

전기영동장치 시스템에 사용되는 콤에 있어서,In a comb used in an electrophoretic system,

몸체부;Body portion;

상기 몸체부의 하부에 형성되는 다수개의 다리로 이루어진 다리부; 및 Leg portion consisting of a plurality of legs formed in the lower portion of the body portion; And

상기 몸체부의 앞면에 일체로 돌출 형성되되, 양측 단부가 상기 몸체부의 양측 단부와 이격되게 형성되는 돌출부;를 포함하여 구성되는 것을 특징으로 한다.It is formed integrally protruding on the front surface of the body portion, both end portions formed to be spaced apart from both ends of the body portion; characterized in that it comprises a.

또한, 본 발명에 따른 멀티콤에서,In addition, in the multicom according to the present invention,

상기 몸체부는 두께를 가진 직사각형 판형상이며, The body portion is a rectangular plate shape having a thickness,

상기 다수개의 다리는 홀수개로 구비되는 것을 특징으로 한다.The plurality of legs is characterized in that the odd number provided.

또한, 본 발명에 따른 멀티콤에서,In addition, in the multicom according to the present invention,

상기 몸체부에는 형태 뒤틀림을 방지하고 미끄러짐을 방지하는 홀을 더 구비하고, 상기 다리부에는 양측 단부에 보조다리를 더 구비하는 것을 특징으로 한다.The body portion is further provided with a hole for preventing the shape distortion and slipping, the leg portion is characterized in that it further comprises auxiliary legs at both ends.

또한, 본 발명에 따른 멀티콤에서,In addition, in the multicom according to the present invention,

상기 돌출부는 측단면의 형상이 상기 몸체부의 앞면으로부터 돌출된 "ㄷ"자 형상인 것을 특징으로 한다.The protrusion is characterized in that the shape of the side cross-section is a "c" shape protruding from the front surface of the body portion.

또한, 본 발명에 따른 멀티콤에서,In addition, in the multicom according to the present invention,

상기 돌출부에는 상기 몸체부에 형성된 홀에 대응하는 홀을 더 구비하는 것을 특징으로 한다.The protrusion may further include a hole corresponding to the hole formed in the body portion.

본 발명의 멀티콤에 따르면, 다양한 형태의 플레이트세트에 구애됨이 없이 간단하면서도 편리하게 사용될 수 있는 효과가 있다.According to the multi-combe of the present invention, there is an effect that can be used simply and conveniently without being bound to various types of plate set.

도 1은 종래 전기영동장치 시스템의 일 실시예의 구성도를 개략적으로 도시한 도면,1 is a view schematically showing a configuration of an embodiment of a conventional electrophoretic device system,

도 2의 (a),(b)는 종래 전기영동장치에 사용되는 두 종류의 콤의 정면도와 AA, BB에서 본 측면도를 나타내는 도면,Figure 2 (a), (b) is a view showing a front view of the two types of combs used in the conventional electrophoretic device and a side view as seen from AA, BB,

도 3은 도 2의 각각의 콤이 세트를 이루는 각각의 플레이트세트에 결합되는 상태를 나타내는 도면,3 is a view illustrating a state in which each comb of FIG. 2 is coupled to each set of plates forming a set;

도 4의 (a)~(e)는 본 발명에 따른 멀티콤의 일실시예의 정면도(a), (a)의 AA에서 본 저면도(b), 배면도(c), (c)의 AA에서 본 평면도(d), B에서 본 우측면도,4A to 4E are bottom views (b), rear views (c) and (c) of the bottom view (a), the rear view (c) of (a) of the front view of one embodiment of the multi-com according to the present invention Top view (d) as seen from the right, the right side view as seen from B,

도 5는 두 종류의 플레이트세트(쇼트플레이트와 스페이서플레이트)를 보여주는 도면,5 shows two kinds of plate sets (short plate and spacer plate),

도 6의 (a),(b)는 본 발명에 따른 멀티콤의 다른 실시예의 사시도,Figure 6 (a), (b) is a perspective view of another embodiment of a multicom according to the present invention,

도 7은 도 6의 (a)의 멀티콤이 도 5의 플레이트세트에 결합되는 상태를 나타내는 도면.FIG. 7 is a view illustrating a state in which the multicom of FIG. 6A is coupled to the plate set of FIG. 5.

이하, 도면들을 참조하여 본 발명의 바람직한 실시예들을 보다 상세하게 설명하기로 한다. 본 발명은 다양한 변경을 가할 수 있고 여러 가지 형태를 가질 수 있는 바, 특정 실시예들을 도면에 예시하고 본문에 상세하게 설명하고자 한다. 그러나, 이는 본 발명을 특정한 개시 형태에 대해 한정하려는 것이 아니며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변경, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. 각 도면을 설명하면서 유사한 참조부호를 유사한 구성요소에 대해 사용하였다. Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the drawings. As the inventive concept allows for various changes and numerous embodiments, particular embodiments will be illustrated in the drawings and described in detail in the text. However, this is not intended to limit the present invention to the specific disclosed form, it should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the drawings, similar reference numerals are used for similar elements.

도 4의 (a)~(e)는 본 발명에 따른 멀티콤의 일실시예의 정면도, 배면도, 평면도, 저면도, 우측면도, 도 5는 두 종류의 플레이트세트(쇼트플레이트와 스페이서플레이트)를 보여주는 도면, 도 6의 (a),(b)는 본 발명에 따른 멀티콤의 다른 실시예의 사시도, 도 7은 도 6의 (a)의 멀티콤이 도 5의 플레이트세트에 결합되는 상태를 나타내는 도면이다.4 (a) to (e) is a front view, a back view, a top view, a bottom view, a right side view of an embodiment of a multi-com according to the present invention, Figure 5 shows two kinds of plate sets (short plate and spacer plate) Figure 6 (a), (b) is a perspective view of another embodiment of a multicom according to the present invention, Figure 7 is a view showing a state in which the multicomb of Figure 6 (a) is coupled to the plate set of Figure 5 .

도 4, 도 6을 참조하면, 본 실시예에 의한 멀티콤(100, 100')은 전기영동장치 시스템에 사용되는 콤으로, 플레이트세트 사이의 여분공간에 주입된 축적겔 위치에 상방향에서 하방향으로 꽂아 사용되는 것으로, 몸체부(10), 몸체의 하부에 형성된 다수개의 다리로 이루어진 다리부(20) 및 몸체부의 앞면에 일체로 돌출 형성되되, 양측 단부가 몸체부의 양측 단부와 일정거리 이격되게 형성된 돌출부(30)를 포함하여 구성된다.4 and 6, the multi-com (100, 100 ') according to the present embodiment is a comb used in the electrophoretic system, the top of the accumulating gel injected into the extra space between the plate set from the top direction It is used to plug into, the body portion 10, the leg portion 20 consisting of a plurality of legs formed in the lower portion of the body and protrude integrally formed on the front surface of the body portion, both ends are spaced apart from both ends of the body portion by a certain distance It is configured to include a protrusion 30 formed.

몸체부(10)는 소정 두께를 가진 직사각형 판형상이며, 형태 뒤틀림을 방지하고 미끄러짐을 방지할 수 있는 홀(H)을 더 구비할 수 있다. The body portion 10 may have a rectangular plate shape having a predetermined thickness, and may further include a hole H that prevents form distortion and prevents slipping.

다리부(20)는 몸체의 하부에 편형상의 다수개의 다리가 소정간격으로 형성되는 것으로, 원하는 약실의 갯수에 맞추어 형성된다. 다리의 개수는 통상적으로 짝수개의 약실을 형성하도록 홀수개로 구비된다. 또한, 다리부의 양측 단부에는 길이가 상대적으로 짧고 폭이 좁으며 소정각도 외측으로 향하는 보조다리(20a)를 더 구비할 수 있음은 물론이다. 보조다리는 콤이 플레이트세트 사이의 여분공간에 투입시 이를 용이하게 하며, 여분공간에 채워진 축적겔이 마르는 것을 방지한다.Leg portion 20 is formed in a plurality of legs in a predetermined interval at the lower portion of the body, is formed in accordance with the desired number of chambers. The number of legs is typically provided in odd numbers to form an even number of chambers. In addition, both ends of the leg portion may be further provided with a secondary leg (20a) is relatively short in length, narrow in width and facing outward a predetermined angle. The secondary leg facilitates this when the comb is placed in the extra space between the plate sets and prevents the accumulation gel filled in the extra space from drying out.

돌출부(30)는 상부 몸체(10)의 앞면에 일체로 형성되되 돌출부의 양측단부가 몸체의 양측 단부와는 일정거리(L) 이격되게 형성되는 것으로, 측단면의 형상은 몸체부의 앞면으로부터 돌출된 "ㄷ"자 형상이고, 몸체부에 형성된 홀에 대응하여 장방형홀(H)을 구비할 수 있다. 홀의 형상이 장방형홀에 한정되는 것이 아님은 물론이며, 다양한 형태로 구비될 수 있다.Protruding portion 30 is integrally formed on the front surface of the upper body 10, the both ends of the protrusion is formed to be spaced apart a predetermined distance (L) from both ends of the body, the shape of the side cross-section protrudes from the front of the body portion It may have a “c” shape and may have a rectangular hole H corresponding to the hole formed in the body portion. Of course, the shape of the hole is not limited to the rectangular hole, and may be provided in various forms.

돌출부는 멀티콤을 플레이트세트 사이의 여분공간에 상방향에서 하방향으로 꽂거나 빼는 것을 용이하게 하고, 멀티콤이 여분공간 사이로 빠지는 것을 방지한다.The protrusion facilitates plugging or unloading the multicomb in the extra space between the plate sets in the upward direction and downward, and prevents the multicom from falling into the extra space.

또한, 돌출부가 몸체부의 양측 단부와 일정거리(L) 이격되게 형성됨으로써, 도 5의 (a),(b)에 도시된 'ㅡ'자형 쇼트플레이트를 구비한 플레이트세트(50,60) 및 'ㄷ'자형 쇼트플레이트를 구비한 플레이트세트(50,60')에 모두 사용될 수 있게 된다.In addition, the protrusions are formed to be spaced apart from the both ends of the body portion by a predetermined distance (L), the plate set (50, 60) and 'with the'-'shaped short plate shown in (a), (b) of FIG. It can be used for both the plate set (50, 60 ') having a'-shaped short plate.

도 5(a)에는 'ㅡ'자형 쇼트플레이트(60)를 구비한 플레이트세트(50,60)가 도시되어 있고, 도 5(b)에는 'ㄷ'자형 쇼트플레이트(60')를 구비한 플레이트세트(50,60')가 도시되어 있다. 도면에서 알 수 있듯이, 각 플레이트세트의 스페이서플레이트(50)에 부착되어 있는 스페이서(50a)의 상부 내측모서리는 미세크기의 소정각도로 경사지게 형성되어 있다. 이는 콤 투입을 용이하게 하기 위함이다.FIG. 5 (a) shows plate sets 50 and 60 with '-' shaped short plates 60, and FIG. 5 (b) shows plates with 'c' shaped short plates 60 '. Sets 50 and 60 'are shown. As can be seen from the figure, the upper inner edge of the spacer 50a attached to the spacer plate 50 of each plate set is formed to be inclined at a predetermined angle of a fine size. This is to facilitate the comb input.

도 7 및 도 8은 본 발명에 따른 멀티콤을 각 플레이트세트에 끼움결합하는 것을 도시한 상태도로서, 도시된 바와 같이 본 발명에 따른 멀티콤은 플레이트세트의 형태에 관계없이 사용할 수 있다.7 and 8 is a state diagram showing the fitting of the multicom according to the present invention to each plate set, as shown, the multicom according to the present invention can be used regardless of the shape of the plate set.

본 발명은 다양한 형태의 플레이트세트에 구애됨이 없이 간단하면서도 편리하게 사용될 수 있으므로, 실험실용의 수직형 단백질 전기영동장치 시스템에 사용할 수 있는 산업상 이용가능성을 갖는다.The present invention can be used simply and conveniently without being bound to various types of plate sets, and thus has industrial applicability for use in laboratory vertical protein electrophoresis systems.

본 발명에서는 실시예들을 참조하여 설명하였지만, 해당 기술 분야의 당업자는 하기의 특허청구범위에 기재된 본 발명의 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 및 변경시킬 수 있음을 이해할 수 있을 것이다.Although the present invention has been described with reference to the embodiments, those skilled in the art may understand that the present invention may be variously modified and changed without departing from the spirit and scope of the present invention as set forth in the claims below. There will be.

Claims (5)

전기영동장치 시스템에 사용되는 콤에 있어서,In a comb used in an electrophoretic system, 몸체부;Body portion; 상기 몸체부의 하부에 형성되는 다수개의 다리로 이루어진 다리부; 및 Leg portion consisting of a plurality of legs formed in the lower portion of the body portion; And 상기 몸체부의 앞면에 일체로 돌출 형성되되, 양측 단부가 상기 몸체부의 양측 단부와 이격되게 형성되는 돌출부;를 포함하여 구성되는 것을 특징으로 하는 멀티콤.And protruding parts integrally formed on the front surface of the body part, the both ends of which are formed to be spaced apart from both ends of the body part. 제1항에 있어서, The method of claim 1, 상기 몸체부는 두께를 가진 직사각형 판형상이며, The body portion is a rectangular plate shape having a thickness, 상기 다수개의 다리는 홀수개로 구비되는 것을 특징으로 하는 멀티콤.The plurality of legs are multicom characterized in that provided in the odd number. 제2항에 있어서, The method of claim 2, 상기 몸체부에는 형태 뒤틀림을 방지하고 미끄러짐을 방지하는 홀을 더 구비하고, 상기 다리부에는 양측 단부에 보조다리를 더 구비하는 것을 특징으로 하는 멀티콤.The body portion further includes a hole for preventing shape distortion and preventing slipping, and the leg portion further includes auxiliary legs at both end portions. 제1항에 있어서, The method of claim 1, 상기 돌출부는 측단면의 형상이 상기 몸체의 앞면으로부터 돌출된 "ㄷ"자 형상인 것을 특징으로 하는 멀티콤.The protrusion is a multi-comb, characterized in that the shape of the side cross-section is a "c" shape protruding from the front of the body. 제3항에 있어서, The method of claim 3, 상기 돌출부에는 상기 몸체부에 형성된 홀에 대응하는 홀을 더 구비하는 것을 특징으로 하는 멀티콤.The protruding portion further comprises a hole corresponding to the hole formed in the body portion.
PCT/KR2014/004066 2014-04-04 2014-05-08 Multi-comb Ceased WO2015152458A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20-2014-0002779 2014-04-04
KR2020140002779U KR200480488Y1 (en) 2014-04-04 2014-04-04 Multi Comb

Publications (1)

Publication Number Publication Date
WO2015152458A1 true WO2015152458A1 (en) 2015-10-08

Family

ID=54240755

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2014/004066 Ceased WO2015152458A1 (en) 2014-04-04 2014-05-08 Multi-comb

Country Status (2)

Country Link
KR (1) KR200480488Y1 (en)
WO (1) WO2015152458A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4215121A4 (en) * 2020-09-16 2024-08-14 Bionlifescience, Inc. SAMPLE COLLECTION PEN
EP4215120A4 (en) * 2020-09-16 2024-07-24 Bionlifescience, Inc. Sample collection stick

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843295A (en) * 1996-12-19 1998-12-01 Pharmacia Biotech Gel electrophoresis well-forming and loading-guide comb
JPH11174025A (en) * 1997-12-12 1999-07-02 Tefco Kk Specimen feed device for electrophoresis analysis
KR20040104495A (en) * 2002-03-07 2004-12-10 미라도르 디엔에이 디자인 인코포레이티드 Apparatus for the manufacture of a disposable electrophoresis cassette and method thereof
US20060237317A1 (en) * 1999-02-12 2006-10-26 Bio-Rad Laboratories Rapid assembly casting system for slab gels
US20130056909A1 (en) * 2011-02-24 2013-03-07 Bio-Rad Laboratories, Inc. Dimensional stabilization of slab gel cassettes to prevent distortion caused by swelling gels

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843295A (en) * 1996-12-19 1998-12-01 Pharmacia Biotech Gel electrophoresis well-forming and loading-guide comb
JPH11174025A (en) * 1997-12-12 1999-07-02 Tefco Kk Specimen feed device for electrophoresis analysis
US20060237317A1 (en) * 1999-02-12 2006-10-26 Bio-Rad Laboratories Rapid assembly casting system for slab gels
KR20040104495A (en) * 2002-03-07 2004-12-10 미라도르 디엔에이 디자인 인코포레이티드 Apparatus for the manufacture of a disposable electrophoresis cassette and method thereof
US20130056909A1 (en) * 2011-02-24 2013-03-07 Bio-Rad Laboratories, Inc. Dimensional stabilization of slab gel cassettes to prevent distortion caused by swelling gels

Also Published As

Publication number Publication date
KR20150003756U (en) 2015-10-14
KR200480488Y1 (en) 2016-05-31

Similar Documents

Publication Publication Date Title
WO2014185583A1 (en) Leakage prevention gel casting device used in vertical protein electrophoresis system
US5013420A (en) Gel electrophoresis/electro-blot apparatus
BRPI0504132A (en) system and method for multi-level billiards in interferometric modulation
WO2015152458A1 (en) Multi-comb
US5656145A (en) Needle guide for loading samples into a vertical slab gel
GB2627085B (en) Methods and systems for analysing nucleic acid molecules
JP3836841B2 (en) Slab gel casting insert and apparatus using the same
Rio et al. Polyacrylamide gel electrophoresis of RNA
ES2617859T3 (en) Disposable container holder to treat biological samples in cytocentrifuges
US6569306B1 (en) Cassette for gel electrophoresis having solid buffer reservoirs
JPH076945B2 (en) Electrophoretic separation method
EP2923198A1 (en) Gel electrophoresis device for loading large sample volumes
Ryan et al. Separation of histone variants and post‐translationally modified isoforms by triton/acetic acid/urea polyacrylamide gel electrophoresis
CA2427025A1 (en) Gel for electrophoresis
US8282803B2 (en) Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
CN106051863A (en) Exhaust hood, oil cup installation assembly of exhaust hood, and oil cup installation structure of exhaust hood
CN212483448U (en) Electrophoresis tank
US5518604A (en) Buffer shaping device
CN106129281B (en) A kind of battery module with battery protective cover
ATE498682T1 (en) TWO-DIMENSIONAL STRAND SHAPE AND LENGTH-DEPENDENT SEPARATION OF NUCLEIC ACID FRAGMENTS
CN221686413U (en) LED module aging rack
CN212505119U (en) A tool for miniature part electrophoresis
bin Yusuf al-Jedaie Genomics in the Light of Scritural References in the Quran and Sunna
WO2016068497A1 (en) Battery module and manufacturing method thereof
CN205749634U (en) A kind of electromechanical engineering circuit tester

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14887995

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 14887995

Country of ref document: EP

Kind code of ref document: A1