WO2015039642A1 - Cardboard inserts for reagent vessel holders for processing in a fully automated elisa device - Google Patents

Abstract

The invention relates to an insert for reagent vessel holders for processing in a fully automated ELISA device. The fields of application are biotechnology, molecular biology, medicine, and pharmaceutics. The problem addressed by the invention is that of providing an economical insert for reagent vessel holders for fully automated ELISA devices. Said claimed inventions allows costs and work time to be reduced by means of the invention. The claimed invention can be used both as a retainer and as packaging by the end customer. The production is simple and economical and also the cardboard packaging is an environmentally friendly and recyclable material.

Description

 Cardboard inserts for reagent container holders for processing in a fully automatic ELISA machine

The invention relates to an insert for reagent container holder for processing in a fully automated ELISA. Areas of application are biotechnology, molecular biology, medicine and pharmacy.

An ELISA test is a versatile antibody-based detection method for biological substances. It allows the examination of a large amount of samples, which suggests the use of fully automatic machines.

With a fully automatic ELISA machine, a large number of samples can be processed quickly and precisely. These include, among other things, automated pipetting, adjustment of the reaction conditions, as well as the automatic evaluation of the reaction results.

For example, in medical diagnostics, automated ELISA tests have become indispensable. The purchase of a fully automatic machine is very costly, so it is endeavored to make the fully automatic for a variety of reactions and samples usable. For this purpose, various components are offered that can be used in a modular way in the ELISA fully automatic machines, in order to use them for different purposes

Harness applications.

Even if these modules increase the area of application of a machine, their purchase is also expensive and material-consuming.

So far, it was for the filling of Regenzien in an ELISA fully automatic

required, the individual reagent vessels in an extra manufactured blank out

Introduce plastic material. These blanks are cost intensive and must be made to fit each kit exactly. Object of the present invention is to provide a cost-effective

Insert for reagent container holder for ELISA fully automatic machines.

This object is achieved according to claims 1-21.

Surprisingly, it has been found that such a blank can be completely replaced as an insert for reagent container holder by an insert made of cardboard.

In particular, the insert according to the invention can be used for the Agility® machine from Dynex Technologies, this is offered in 3 variants:

 Bronze: Reagents must be bottled and then put in

Plastic inserts (SmartKits Dynex) are positioned.

 Silver: The reagents can remain in the original bottles and are positioned in appropriate plastic inserts (SmartKits from Dynex).

Gold: Reagents are ready-to-use in one application and can be placed directly from the outer packaging in the device.

 The use according to the invention for the gold variant of the Agility® ELISA fully automatic machine can be used in particular.

Reagent vessel holder according to the invention refers to a holding box for an insert or blank, which fixes the reagent containers containing the reagents within this holder box. The reagent container holder can be used accurately in the ELISA full automatic, so that the reagents contained in it can be processed in the machine.

Also shown in Figures 1 and 2, here is shown the reagent container holder of the Agility® fully automatic machine from Dynex Technologies together with the cardboard insert according to the invention.

The design of the inserts according to the invention is individual to different

Product ranges, as different bottle sizes, sizes and shapes are available in the various kits. On the surface of the cardboard insert any recesses can be introduced whose position, size and shape is freely selectable. The machine is programmed to recognize these holders for various types of reagent containers.

An essential prerequisite for the use of cardboard according to the invention is its stability. The bottom of the cardboard box must have a certain strength so that the vials do not slip or be pushed downwards, so that the necessary measurement by the machine can not be influenced and the bottles can be filled correctly. The stability of the bottom holding the vial must be particularly robust. Here it is important that the distance in the height (Z direction) does not change. The Z direction has an influence on the volume calculation in the vials.

The ELISAAutomat takes with the pipetting a defined volume from the respective reaction vessels, after the level by a

Resistance measurement was determined. Due to the difference in height, this

Volume decrease is then causes the remaining volume (with known

Bottle dimensions on the relationship between diameter / height / volume) and thus also the maximum possible immersion depth until reaching the vessel bottom. Here, in a preferred application, a buffer of 3 mm is set. Leaning bottles lead to the fact that through the

Volume decrease a wrong height difference is determined and therefore the

Residual volume or the number of possible individual withdrawals are calculated incorrectly. Furthermore, the pipetting unit can be placed on the bottom of the bottle. That's why the bottles have to stand on a stable floor.

Such a stable floor is achieved through the material of the cardboard box insert and inner shelves should be necessary by adhering these floors to the front and back sides of the cardboard box (see Figures 8 and 13).

The inner floors are glued by means of a tab of preferably at least 0.5 cm wide. The material of the insert according to the invention consists of cardboard with a density of at least 200 g / m ³ . Preferably, a paperboard material of density of at least 300 g / m ^{3 is} used, and most preferred is one

Kartonagematerial used with a density of at least 400 g / m ³ .

It must be ensured in the choice of material that this remains well foldable despite everything to ensure the advantages of the invention. Optimal is therefore a density of 400g / m ³ .

According to the invention, the cardboard insert has a shield, which during the

Inserted into the reagent container holder is folded and on which a code is applied, which contains the necessary information for the fully automatic machine, so that the right program can be selected. In a preferred embodiment, after insertion into the reagent container holder, the visible area of the plate is between 2.2 cm and 10.3 cm in height. The width of the label is covered horizontally by 1, 1 cm from both sides by the reagent container holder.

In detail:

The invention relates to a foldable insert for reagent container holder for processing in a fully automatic ELISA, characterized in that this

Cardboard material consists, and after folding, of a parallelepiped with the external dimensions of length (1) of 13 cm to 13.2 cm, the width (2) of 7.1 cm to 7.3 cm and the height (3) of 2 cm to 11.4 cm results, so that the employment accurately in one

The insert at the front (4) as a folding shield (5) with a width (6) of 4.1 cm to 7.3 cm and a height (7) of 2.1 cm to 11, 4 cm is formed or contains such a plate (5) on which a barcode can be applied; and wherein further recesses can be introduced on the upper side (8) of the cuboid. Preferably, the insert is characterized in that this after folding a cuboid with the external dimensions of the length (1) of 13.1 cm, the width (2) of 7.1 cm to 7.2 cm and the height (3) of 2 cm to 11, 3 cm results. In one embodiment variant, the ELISA fully automatic machine is an Agility® ELISA fully automatic machine.

In a further embodiment of the invention, the insert is characterized

characterized in that the shield has a height of 2.1 cm to 11, 4 cm.

It is further preferred that the shield (5) contains a barcode.

In a particularly preferred embodiment of the invention, the insert is characterized in that after folding the cuboid contains at least one inner bottom.

In this case, the at least one inner bottom preferably has a distance from the surface of the cuboid (8) of 0.4 cm to 11 cm. In a further embodiment of the invention, in the insert after folding on the upper side (8) of the cuboid, recesses which are adapted to size, number, shape and position corresponding to the reagent vessels to be processed can be introduced.

It is preferred that the recesses are colored at the borders according to the reagents to be adjusted. This results in an advantage for the manufacturer during product assembly.

In a preferred embodiment of the invention, the insert is characterized

characterized in that this after folding a cuboid with the external dimensions of the length (1) 13 cm to 13.2 cm, the width (2) of 7 cm to 7.2 cm and the height (3) of 8.1 cm 8.3 cm, wherein the insert on the front side (4) as a foldable shield (5) with a width (2) of the cuboid and a height (7) of 9.9 cm to 10.1 cm is formed or a contains such a shield (5). In a particularly preferred embodiment, the insert is characterized

characterized in that this after folding a cuboid with the outer dimensions of the length (1) of 13.1 cm, the width (2) of 7.1 cm and the height (3) of 8.2 cm, the use of the front (4) as a foldable shield (5) with a width (2) of the cuboid and a height (7) of 10 cm is formed or such a shield (5).

Particularly preferred is an insert in which at the top (8) of the cuboid in each case three circular mismatches in two parallel rows (9) are arranged, wherein the diameter of the two of the front (4) closest circular recesses (10) 1, 5 cm to 1.7 cm and the four of the front side (4) most recessed circular recesses (11) have a diameter of 2.4 cm to 2.6 cm and also the use two, at least partially located under the circular recesses , Inner trays (12, 13), whereby the distance between the two of the front side (4) closest circular recesses (10) and the underlying at least one bottom (12) 2.4 cm to 2.6 cm and the distance between the four circular recesses (11) located farthest from the front side (4) and the at least one bottom (13) below is 3.4 cm to 3.6 cm.

In a further preferred embodiment, the insert is characterized in that the diameter of the two circular recesses (10) closest to the front side (4) is 1.6 cm and the four of the front side (4) are farthest circular recesses (11). have a diameter of 2.5 cm and the distance between the two circular recesses (10) closest to the front side (4) and the bottom (12) below is 2.5 cm and the distance between the four front sides (4 ) most distant circular recesses (11) and the underlying bottom (13) is 3.5 cm.

In a further preferred embodiment, in the insert according to the invention on the upper side (8) of the cuboid, in each case three circular pairings are arranged horizontally in a row (14) and two circular recesses are arranged in one Row (15) vertkal arranged, wherein the diameter of the two of the front (4) closest circular recesses (15) 1, 5 cm to 1, 7 cm and the two outer recesses (14a, 14c) of the horizontal and the front (4) farthest recesses (14) have a diameter of 2.1 cm to 2.3 cm, and the central cutout (14b) of the horizontally and the front (4) farthest recesses (14) has a diameter of 2, 4 cm to 2.6 cm, and furthermore the insert contains two inner plates (16, 17) located at least under a part of the circular recesses, whereby the distance between the two circular plates closest to the front side (4)

Recesses (15) and the underlying floor (16) is 2.4 cm to 2.6 cm and the distance between the three of the front side (4) farthest circular recesses (14) and the underlying bottom (17) 3 , 4 cm to 3.6 cm. According to the invention, an insert is particularly preferred in which on the upper side (8) of the cuboid three circular Auspaarungen in a row (14) are arranged horizontally and two circular recesses in a row (15) are arranged vertkal, wherein the diameter of the two of Front (4) nearest circular recesses (15) is 1, 6 cm and the two outer recesses (14a, 14c) of the horizontal and the front (4) farthest recesses (14) have a diameter of 2.2 cm and the central recess (14b) of the horizontally and the front (4) farthest recesses (14) has a diameter of 2.5 cm and further comprises the use of two, at least partially under the circular recesses located inner floors (16, 17 ), whereby the distance between the two of the front side (4) closest circular recesses (15) and the underlying Bo the (16) is 2.5 cm and the distance between the three

Front (4) of the most recessed circular recesses (14) and the underlying bottom (17) is 3.5 cm.

In an alternative embodiment of the invention, an insert is preferred, which is characterized in that three circular disqualifications in three parallel rows (18) are arranged on the upper side (8) of the cuboid and in each case two circular recesses in two parallel rows (19). are arranged, wherein the diameter of the nine circular recesses (18) closest to the front (4) is 1.5 cm to 1.7 cm and the four outer recesses (19) located farthest to the front (4) have a diameter of 2.4 cm Further, the insert has two, at least under a portion of the circular recesses located, inner floors (20, 21), whereby the distance between the nine of the front (4) closest to the circular

Recesses (18) and the underlying bottom (20) is 2.4 cm to 2.6 cm and the distance between the four of the front side (4) farthest circular recesses (19) and the underlying bottom (21) 3 , 4 cm to 3.6 cm.

According to the invention, in particular an insert is preferred in which in each case three circular mismatches are arranged in three parallel rows (18) on the upper side (8) of the cuboid and in each case two circular recesses are arranged in two parallel rows (19), wherein the diameter of the nine of the

 Front (4) nearest circular recesses (18) is 1, 6 cm and the four outer of the front (4) farthest recesses (19) have a diameter of 2.5 cm and the distance between the nine of the front ( 4) closest to the circular recesses (18) and the underlying bottom (20) is 2.5 cm and the distance between the four of the front (4) most distant circular recesses (19) and the underlying bottom (21) 3.5 cm.

Further preferred is an insert, which can be stored in the unfolded state and after folding results in one of the cuboid of the preceding claims.

In this case, this insert preferably in the unfolded state has external dimensions of a width of 9 cm to 17 cm and a height of 17 cm to 36 cm. According to the invention, the insert is preferably characterized in that the at least one inner bottom is glued to the front and rear side (23) of the cuboid. Particularly preferably, the insert according to the invention consists of cardboard material.

The inventive Kartionageneinsatz is shown by way of example in Figures 1-15. These are various possible embodiments as embodiments which are not exhaustive.

By replacing the blank with a cardboard insert, costs and working time are saved. The insert according to the invention can be used both as a holder and as packaging for the end customer. The production is simple and

cost-effective, moreover, the cardboard is an environmentally friendly and recyclable material. Due to the possibility of folding, the insert according to the invention requires little space during storage, so in this respect also costs and capacities can be saved.

Legend to the pictures:

Figure 1: Reagent vessel holder of the Agility® fully automatic machine from Dynex

 Technologies and cardboard insert individually

Figure 2: Reagent vessel holder of the Agility® fully automatic machine from Dynex

 Technologies with inserted cardboard insert

Figure 3: folded cardboard packaging embodiment

Figure 4: folded cardboard packaging embodiment

Figure 5: folded cardboard packaging embodiment

Figure 6: folded cardboard box embodiment

Figure 7: folded cardboard packaging embodiment

 Figure 8: Folded cardboard packaging example, gluing stable floors to the front and back sides of the cardboard box

 Figure 9: Top view folded cardboard packaging embodiment

Figure 10: folded cardboard box embodiment,

Figure 11: folded cardboard packaging embodiment

Figure 12: folded cardboard packaging embodiment

 Figure 13. unfolded cartonboard embodiment, adhering stable bottoms to the front and back sides of the carton

Figure 14: unfolded cardboard packaging example

Figure 15: unfolded cardboard packaging embodiment

LIST OF REFERENCE NUMBERS

(1) length

 (2) width

 (3) height

 (4) front side

 (5) Shield

 (6) width

 (7) height

 (8) top

 (9) pairings in two parallel rows

(10) recesses

 (11) recesses

 (12) inner ground

 (13) inner ground

 (14 a-c) recesses

 (15) recesses

 (16) inner ground

 (17) inner ground

 (18) recesses

 (19) recesses

 (20) inner ground

 (21) inner ground

 (22) Adhesive surface inner floors

 (23) Front and back cuboid

 (24) Reagent vessel holder

 (25) Cardboard insert

Claims

 claims 1. Foldable insert for reagent container holder for processing in a fully automatic ELISA, characterized in that it consists of cardboard material and after folding a cuboid with the external dimensions of the length (1) of 13 cm to 13.2 cm, the width (2) from 7.1 cm to 7.3 cm and the height (3) from 2 cm to 11, 4 cm results, making the insert perfectly fitting in one  The insert at the front (4) as a folding shield (5) with a width (6) of 4.1 cm to 7.3 cm and a height (7) of 2.1 cm to 11, 4 cm is formed or contains such a plate (5) on which a barcode can be applied; and wherein further recesses can be introduced on the upper side (8) of the cuboid. 2. Use according to claim 1, characterized in that this after folding a cuboid with the external dimensions of the length (1) of 13.1 cm, the width (2) of 7.1 cm to 7.2 cm and the height ( 3) from 2 cm to 11, 3 cm. 3. Use according to one of claims 1-2, characterized in that it is the ELISA fully automatic machine is an Agility® ELISA fully automatic. 4. Use according to one of claims 1-3, characterized in that the  Shield has a height of 2,1 cm to 11, 4 cm. 5. Use according to one of claims 1-4, characterized in that the  Shield (5) contains a barcode. 6. Use according to any one of claims 1-5, characterized in that after folding the cuboid contains at least one inner bottom. 7. Use according to one of claim 6, characterized in that the at least one inner bottom has a distance from the surface of the cuboid (8) of 0.4 cm to 11cm. 8. Use according to one of claims 1-7, characterized in that after folding on the upper side (8) of the cuboid recesses, which are adapted according to size, number, shape and position corresponding to the processed reagent vessels, can be introduced. 9. Use according to one of claims 1-8, characterized in that the  Recesses on the borders according to the set  Reagents are colored. Insert according to one of claims 1-9, characterized in that this after folding a cuboid with the external dimensions of the length (1) 13 cm to 13.2 cm, the width (2) of 7 cm to 7.2 cm and the height (3) is from 8.1 cm to 8.3 cm, the insert on the front side (4) acting as a foldable shield (5) with a width (2) of the cuboid and a height (7) of 9 , 9 cm to 10.1 cm or contains such a shield (5). 11. Use according to one of claims 1-10, characterized in that this after folding a cuboid with the external dimensions of the length (1) of 13.1 cm, the width (2) of 7.1 cm and the height (3 ) of 8.2 cm, wherein the insert on the front side (4) as a foldable shield (5) with a width (2) of the cuboid and a height (7) of 10 cm is formed or such a shield (5) contains. 12. Use according to any one of claims 1-11, characterized in that on the upper side (8) of the cuboid in each case three circular recesses in two parallel rows (9) are arranged, wherein the diameter of the two of the front (4) located closest circular recesses (10) is 1, 5 cm to 1, 7 cm and the four of the front (4) farthest circular recesses (11) have a diameter of 2.4 cm to 2.6 cm and further comprising the insert two, at least partially located under the circular recesses, inner floors (12, 13), whereby the distance between the two of the front (4) nearest circular recesses (10) and the underlying at least one bottom (12) is 2.4 cm to 2.6 cm and the distance between the four Front (4) located at the most distant circular recesses (11) and the underlying at least one bottom (13) 3.4 cm to 3.6 cm. 13. Insert according to one of claims 1-12, characterized in that the diameter of the two of the front side (4) located closest  Circular recesses (10) is 1, 6 cm and the four of the front (4) farthest circular recesses (11) one  Have diameter of 2.5 cm and the distance between the two of the  Front (4) closest to circular recesses (10) and the underlying bottom (12) is 2.5 cm and the distance between the four of the front (4) most distant circular  Recesses (11) and the underlying bottom (13) is 3.5 cm. 14. insert according to any one of claims 1-13, characterized in that on the upper side (8) of the cuboid in each case three circular recesses in a row (14) are arranged horizontally and two circular recesses in a row (15) are arranged vertically, the diameter of the two circular recesses (15) closest to the front side (4) being 1.5 cm to 1.7 cm, and the two outer recesses (14a, 14c) of the recesses arranged horizontally and the front side (4) being furthest apart (14) have a diameter of 2.1 cm to 2.3 cm and the middle  Recess (14b) of the horizontal and the front (4) farthest recesses (14) has a diameter of 2.4 cm to 2.6 cm and further the use of two, located at least under a portion of the circular recesses, inner floors ( 16, 17), whereby the distance between the two of the front side (4) closest circular recesses (15) and the underlying bottom (16) is 2.4 cm to 2.6 cm and the distance between the three of Front (4) of the most recessed circular recesses (14) and the underlying bottom (17) is 3.4 cm to 3.6 cm. 15. Insert according to one of claims 1-14, characterized in that on the upper side (8) of the cuboid in each case three circular recesses in a row (14) are arranged horizontally and two circular recesses in a row (15) vertkal arranged, wherein the diameter of the two of the  Front (4) nearest circular recesses (15) is 1, 6 cm and the two outer recesses (14a, 14c) of the horizontal and the front (4) farthest recesses (14) have a diameter of 2.2 cm and the central recess (14b) of the horizontally and the front (4) farthest recesses (14) has a diameter of 2.5 cm  and further, the insert two, at least partially under the circular  Recessed, inner floors (16, 17) contains, whereby the distance between the two of the front side (4) closest circular recesses (15) and the underlying bottom (16) is 2.5 cm and the distance between the three the front (4) of the most recessed circular recesses (14) and the underlying bottom (17) is 3.5 cm. 16. An insert according to any one of claims 1-15, characterized in that on the upper side (8) of the cuboid in each case three circular recesses in three parallel rows (18) are arranged and in each case two circular  Recesses are arranged in two parallel rows (19), wherein the  Diameter of the nine closest to the front (4)  circular recesses (18) is 1, 5 cm to 1, 7 cm and the four outer of the front (4) farthest recesses (19) have a diameter of 2.4 cm to 2.6 cm and further the use of two, at least under a portion of the circular recesses located inner floors (20, 21), whereby the distance between the nine of the front (4) closest circular recesses (18) and the underlying bottom (20) 2.4 cm until 2.6 cm and the distance between the four of the front (4) most distant  circular recesses (19) and the underlying bottom (21) is 3.4 cm to 3.6 cm. 17. Insert according to one of claims 1-16, characterized in that on the upper side (8) of the cuboid in each case three circular recesses in three parallel rows (18) are arranged and in each case two circular  Recesses are arranged in two parallel rows (19), wherein the diameter of the nine of the front (4) closest  circular recesses (18) are 1, 6 cm and the four outer of the front (4) farthest recesses (19) have a diameter of 2.5 cm and the distance between the nine of the front (4) closest circular recesses (18) and the underlying floor (20) is 2.5 cm and the distance between the four of the front side (4) farthest circular recesses (19) and the underlying bottom (21) is 3.5 cm. 18. Use according to one of claims 1-17, characterized in that it can be stored in the unfolded state and after folding results in one of the cuboid of the preceding claims. 19. Use according to any one of claims 1-18, characterized in that it has in the unfolded state external dimensions of a width of 9 cm to 17 cm and a height of 17 cm to 36 cm. 20. Insert according to one of claims 1-19, characterized in that the at least one inner bottom is adhered to the front and back (23) of the cuboid. 21. Use according to any one of claims 1-20, characterized in that the insert consists of cardboard material.