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WO2015029673A1 - Dispositif de collecte et dispositif de détection - Google Patents

Dispositif de collecte et dispositif de détection Download PDF

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Publication number
WO2015029673A1
WO2015029673A1 PCT/JP2014/069888 JP2014069888W WO2015029673A1 WO 2015029673 A1 WO2015029673 A1 WO 2015029673A1 JP 2014069888 W JP2014069888 W JP 2014069888W WO 2015029673 A1 WO2015029673 A1 WO 2015029673A1
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WO
WIPO (PCT)
Prior art keywords
collection
housing
substrate
opening
nozzle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2014/069888
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English (en)
Japanese (ja)
Inventor
克佳 高橋
藤岡　一志
伸佳 石野
恵美 肱黒
大樹 奥野
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sharp Corp
Original Assignee
Sharp Corp
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Filing date
Publication date
Application filed by Sharp Corp filed Critical Sharp Corp
Publication of WO2015029673A1 publication Critical patent/WO2015029673A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/24—Suction devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • G01N2001/222—Other features
    • G01N2001/2223—Other features aerosol sampling devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/24—Suction devices
    • G01N2001/245—Fans

Definitions

  • the present invention relates to a collection device and a detection device, and more particularly to a collection device and a detection device for collecting and detecting particles in a fluid.
  • Patent Document 1 As an apparatus for collecting particles in the atmosphere, for example, as disclosed in Japanese Patent Application Laid-Open No. 2000-300466 (hereinafter referred to as Patent Document 1), an apparatus for collecting particles in the atmosphere on a medium in a petri dish, A so-called air sampler is usually used.
  • Patent document 1 is disclosing the airborne microbe sampler which collects the airborne microbe on the petri dish which accommodates a culture medium through the nozzle plate which has a some hole. The air sampler separates particles from air flowing into the aircraft using an inertial collision method and collects the particles on a petri dish.
  • the air sampler as disclosed in Patent Document 1 has the following problems. (1) At the time of collection, airtightness of parts other than the nozzle substrate is required. Therefore, it has a complicated structure for ensuring hermeticity, such as a lock by rotation, and it is difficult to easily replace or remove the petri dish easily or automatically.
  • the present invention has been made in view of such problems, and has utilized a collection device that facilitates handling of a collection substrate while ensuring the ability to collect particles in a fluid, and the collection device.
  • the object is to provide a detection device.
  • the collection device is a device for collecting particles in the fluid introduced into the enclosure on the surface of the collection substrate set in the enclosure.
  • a nozzle substrate that is installed on at least one surface of the housing and has a nozzle drilled therein, an introduction means for introducing a fluid outside the housing into the housing through the nozzle at a predetermined flow rate, and a collection substrate,
  • Driving means for moving between a first position outside the housing and a second position inside the housing, the surface of which is the front in the direction of nozzle drilling, through an opening provided in the housing
  • a resistance means for making the resistance to the fluid passing through the opening of the housing larger than the resistance to the fluid passing through the nozzle when the collection substrate is in the second position.
  • the resistance means is a fixing portion that covers a portion other than the drive means of the opening in a state where the drive means exists in the opening of the housing when the collection substrate is in the second position.
  • a member for increasing the resistance to the fluid is installed at a position where the fixed portion and the opening are in contact with each other.
  • the driving means is located outside the housing, and the resistance means is a lid that covers the opening of the housing.
  • a detection device includes the above-described collection device and a detector for detecting particles collected on the surface of the collection substrate, and the driving means includes the collection substrate. Move to the detector.
  • the detector includes a light source for irradiating excitation light and a light receiving element for receiving fluorescence
  • the driving means sets the surface of the collection substrate after the collection substrate is set to the second position. It moves to the 3rd position where excitation light is irradiated from a light source.
  • the detection device further includes a heater, and the driving means moves the collection substrate to the third position after a predetermined time.
  • the collection substrate can be easily handled while securing the ability to collect particles in the fluid in the collection device. Further, by using the collection device, it is possible to detect particles in the fluid with high accuracy by the detection device and easy handling of the collection substrate.
  • the collection device collects particles in a fluid such as air introduced into the enclosure on the surface of a collection substrate set in the enclosure.
  • FIG. 1 and FIG. 2 are schematic views showing a specific example of the configuration of the collection device 100 according to the first embodiment.
  • the collection device 100 includes a nozzle substrate 2 installed on one surface (upper surface in FIGS. 1 and 2) of the housing 1 and a nozzle 2 ⁇ / b> A drilled in the nozzle substrate 2.
  • the fan 3 which is an example of an introduction mechanism for introducing air, which is a fluid outside the casing 1, into the casing 1 at a predetermined flow rate via the first position and the casing 1 outside the casing 1.
  • the collection substrate 200 is moved via the opening 1A provided in the housing 1 between the second position where the surface of the collection substrate 200 is in front of the nozzle 2A in the drilling direction.
  • a fixture 61 as an example of a resistance mechanism for increasing the resistance to air passing through 2A.
  • the collection device 100 further includes a control device 10 having a CPU (Central Processing Unit) 10A.
  • the control device 10 is electrically connected to the drive unit 5 and the fan 3 to control their drive.
  • the fan 3 is provided on the surface opposite to the surface on which the nozzle substrate 2 of the housing 1 is provided (the lower surface in FIGS. 1 and 2).
  • the fan 3 rotates under the control of the control device 10 to generate a negative pressure in the housing 1, thereby introducing outside air into the housing 1 through the nozzle 2 ⁇ / b> A.
  • the control device 10 rotates the fan 3 by a specified rotation amount by driving the fan 3 by a predetermined control amount. Thereby, outside air is introduced into the housing 1 at a predetermined flow rate.
  • the introduction mechanism is not limited to a fan, and may be a pump or the like.
  • the collection device 100 further includes a holding unit 4 for holding the collection substrate 200.
  • the collection substrate 200 may be held by being mounted on the holding unit 4 or may be fixed with screws or the like.
  • FIG. 1 shows a state in which the collection substrate 200 is in the first position
  • FIG. 2 shows a state in which the collection substrate 200 is in the second position.
  • 1 and 2 show the drive unit 5.
  • the drive unit 5 supports the holding unit 4 with an arm, and moves the collection substrate 200 held by the holding unit 4 between the first position and the second position via the opening 1A. .
  • the fixture 61 which is a fixing portion covers a portion other than the arm of the opening 1A in a state where the arm of the driving unit 5 exists in the opening 1A when the collection substrate 200 is in the second position. That is, the fixture 61 has a surface wider than the opening 1A.
  • the fixture 61 is provided at an end portion of the holding portion 4 far from the opening 1A, and is fixed when inserted into the housing 1 so that the collection substrate 200 held by the holding portion 4 is set to the second position.
  • a tool 61 covers the opening 1A.
  • the drive unit 5 maintains the pressure in a direction for pressing the fixture 61 against the opening 1A for a specified time.
  • the collection substrate 200 may be a resin, glass, metal, silicon substrate, or a thin plate such as polydimethylsiloxane (PDMS) formed on a substrate such as silicon or resin.
  • PDMS polydimethylsiloxane
  • carriers, such as a petri dish, may be sufficient. Even if it is any form, the holding
  • FIG. 3 is a diagram illustrating a specific example of the external appearance of the drive unit 5 and the holding unit 4.
  • the drive unit 5 has an arm extending in the horizontal or substantially horizontal direction, and the holding unit 4 is supported by the arm.
  • the drive unit 5 is configured to be movable in the support direction of the holding unit 4.
  • FIG. 4 is a diagram showing a specific example of the appearance of the holding unit 4.
  • the holding unit 4 has a surface for holding the collection substrate 200, and the collection substrate 200 is mounted on the surface or fixed with screws or the like.
  • a fixture 61 is provided at an end portion of the holding portion 4 far from the opening portion 1A in parallel or substantially in parallel with the opening portion 1A of the housing 1.
  • An O-ring 62 is installed as an example of a member for increasing the resistance to air at a position where the fixture 61 is in contact with the opening.
  • the O-ring 62 may be provided on the opening 1A side.
  • FIG. 5 is a diagram showing another example of the drive unit 5.
  • the drive unit 5 is not limited to one that is movable only in the one-axis direction shown in FIG. 3, and may be movable in three-axis directions as shown in FIG.
  • FIG. 6 is a diagram illustrating a specific example of the appearance of the housing 1 and the fan 3.
  • FIG. 6 shows an example, and the housing 1 has a box shape.
  • the nozzle substrate 2 is fitted on the upper surface of the housing 1, and has an opening on the side surface (opening 1A).
  • a fan 3 is installed on the bottom surface.
  • FIG. 7 is a diagram showing a specific example of the nozzle substrate 2.
  • the nozzle substrate 2 is obtained by forming one or more nozzles 2A on a metal substrate such as stainless steel or aluminum.
  • the material of the nozzle substrate 2 is not limited to a specific material.
  • stainless steel, resin, glass, and other metals are preferably used.
  • the diameter of the nozzle (hole) 2A is about 0.01 mm to 10 mm, and the nozzle length (that is, the thickness of the nozzle substrate 2) is about 0.1 mm to 50 mm.
  • the distance from the nozzle substrate 2 to the collection substrate 200 is preferably about 0.01 mm to 10 mm.
  • the shape of the nozzle 2A is not limited.
  • the shape of the nozzle 2A may be, for example, a circle as shown in FIG. 7 or a slit shape. In addition, a taper shape etc. may be sufficient. Also, the number is not limited and may be about 1 to 1000. As shown in FIG. 7, the one or more nozzles 2A are preferably drilled in a predetermined range. Thereby, particles can be collected in a predetermined range on the collection substrate 200, and the efficiency is high in the detection described later.
  • FIG. 8A is a diagram showing a state of setting the collection substrate 200 to the housing 1.
  • the collection substrate 200 is supported by the arm of the drive unit 5 while being held horizontally or substantially horizontally by the holding unit 4, and is horizontally or substantially horizontal to the opening 1 A of the housing 1.
  • the opening 1A is perpendicular or substantially perpendicular to the insertion direction, and the fixture 61 formed in parallel with the opening 1A covers the opening 1A.
  • the drive unit 5 is a drive type as shown in FIG. 3 or FIG. 5, the collection substrate 200 can be accurately positioned with respect to the nozzle substrate 2.
  • FIG. 8B is a diagram schematically showing the state of the opening 1A when the collection substrate 200 is in the second position.
  • the fixture 61 covers the opening 1 ⁇ / b> A of the housing 1, and the fixture 61 is pressed against the opening 1 ⁇ / b> A by the pressing force of the drive unit 5.
  • An O-ring 62 is sandwiched between the fixture 61 and the opening 1A. Thereby, the opening 1A is sealed.
  • FIG. 9 is a schematic diagram for explaining the collection principle of the collection device 100.
  • the collection device 100 collects particles in the air on the surface of the collection substrate 200 using an inertial collision method.
  • the collection substrate 200 is set so as to be parallel or substantially parallel to the nozzle substrate 2.
  • the fan 3 may be one that takes outside air into the housing 1 by exhausting the air inside the housing 1, or directly introduces outside air into the housing 1.
  • the collection substrate 200 is disposed between the fan 3 and the nozzle substrate 2 on the suction side of the fan 3.
  • the nozzle substrate 2 is disposed between the fan 3 and the collection substrate 200 on the exhaust side of the fan 3.
  • the fan 3 is the former one.
  • FIG. 10 is a diagram schematically showing the air flow in the housing.
  • the fan 3 is driven with the collection substrate 200 set at the second position and the opening 1A covered with the fixture 61
  • the outside air at the flow rate Q N is introduced into the housing 1 via the nozzle 2A.
  • the outside air of the flow rate Q A is introduced into the housing 1 through the opening 1A covered with the fixture 61.
  • the flow rate Q N passing through the nozzle 2A in the flow rate Q s on the surface of the collection substrate 200 needs to be dominant. is there.
  • the ratio T is preferably greater than 0.9, more preferably greater than 0.99.
  • the laminar flow has arisen in the housing 1.
  • the Reynolds number Re defined by the ratio between the inertial force and the viscous force satisfies Re ⁇ 2300.
  • the fluid resistance R is calculated using the Hagen-Poiseuille equation. That is, when the nozzle 2A is a circular tube with a radius r, the flow rate Q is given by assuming that the nozzle length (same as the thickness of the nozzle substrate 2) is L, the pressure difference between both ends of the nozzle is ⁇ P, and the air viscosity is ⁇ .
  • Q ⁇ ⁇ r 4 ⁇ ⁇ P / (8 ⁇ ⁇ L).
  • the fluid resistance R of the nozzle substrate 2 is proportional to the nozzle length L and inversely proportional to the nozzle diameter r (the fourth power).
  • the resistance mechanism for making the fluid resistance of the opening 1A larger than the fluid resistance of the nozzle 2A in the state covered with the fixture 61 in the collection device 100 is the fluid resistance R N of the nozzle substrate 2 described above.
  • the following four methods can be adopted as the resistance mechanism.
  • Method 1 The flow passage cross-sectional area (or flow passage diameter) in the opening 1 ⁇ / b> A covered with the fixture 61 is made smaller (thinner) than the cross-sectional area of the nozzle 2 ⁇ / b> A of the nozzle substrate 2.
  • Method 2) Lengthening the flow path in the opening 1 ⁇ / b> A covered with the fixture 61.
  • Method 3) Increasing the contact area between the air and the wall surface in the flow path in the opening 1A covered with the fixture 61 (folding, providing unevenness, etc.)
  • Method 4) Sites that are rapidly expanded and contracted are provided in the flow path in the opening 1A covered with the fixture 61.
  • the fluid cross-sectional area (or the channel diameter) of the air introduced into the opening 1A via the gap between the fixture 61 and the housing 1 is reduced (thinned).
  • the control device 10 performs a predetermined period after the collection substrate 200 is set to the second position during the collection period, with respect to the fixture 61 provided at the end of the holding unit 4 by the drive unit 5.
  • the drive unit 5 is controlled so as to continuously apply a pressing force in the direction from the outside to the inside of the housing 1 and press it against the housing 1.
  • a rubber-like member is provided at the contact portion between the opening 1A and the fixture 61, and the control device 10 is held by the drive unit 5 during the collection period.
  • the drive unit 5 is controlled so as to press the fixture 61 provided at the end of the unit 4 against the housing 1 until the rubber member is deformed.
  • FIG. 11 is a diagram for explaining a resistance mechanism employing the method 2 described above.
  • FIG. A thick line arrow in the figure is a flow path of air introduced into the opening 1A through a gap between the fixture 61 and the housing 1.
  • the fixture 61 is configured to have a large surface area facing the housing 1.
  • FIG. 11B is given as a second example of the resistance mechanism employing the method 2.
  • Thick line arrows in the figure are air flow paths that pass through the opening 1A and are introduced into the housing 1.
  • the housing 1 is configured such that at least the wall thickness of the opening 1A is thick.
  • FIGS. 12 to 14 are diagrams for explaining a resistance mechanism adopting the method 3 described above.
  • FIG. A thick line arrow in the figure is a flow path of air introduced into the opening 1A through a gap between the fixture 61 and the housing 1.
  • each of the portions where the fixture 61 and the housing 1 face each other has an uneven structure.
  • the flow path in which the fixture 61 and the housing 1 are engaged and bent is formed by the above-described uneven structure.
  • a comb-shaped mechanism may be used.
  • the size and direction of the concavo-convex structure may vary.
  • the flow path may be lengthened and the contact area may be increased.
  • fine irregularities may be formed in each of the portions where the fixture 61 and the housing 1 face each other.
  • FIG. 15 is a view for explaining a resistance mechanism adopting the method 4 described above.
  • FIG. A thick line arrow in the figure is a flow path of air introduced into the opening 1A through a gap between the fixture 61 and the housing 1.
  • a protrusion is provided on each of the portions where the fixture 61 and the housing 1 face each other.
  • the flow path cross-sectional area rapidly decreases at the protruding portion and rapidly increases at the portion other than the protrusion.
  • the positions of the protrusions may be such that the housing 1 side is on the outside and the fixture 61 side is on the inside.
  • the fixture 61 as an example of the resistance mechanism is provided at the end of the holding unit 4, and the collection substrate 200 is set to the second position in the housing 1 to automatically In particular, the opening 1A is covered.
  • a fixture may be provided on the housing 1 side.
  • FIG. 16 is a diagram for explaining another example of the fixture.
  • FIG. 16A shows the case where the collection substrate 200 is in the first position
  • FIG. 16B shows the case where the collection substrate 200 is in the second position.
  • a fixture 63 that is a lid that covers the opening 1 ⁇ / b> A is provided in the vicinity of the opening 1 ⁇ / b> A of the housing 1.
  • the fixture 63 is a movable type capable of opening and closing the opening 1A, and a mechanical shutter such as an electronic shutter is preferably used.
  • the driving mechanism of the fixture 63 is electrically connected to the control device 10, and its opening / closing is controlled by the control device 10.
  • FIG. 16A shows the case where the collection substrate 200 is in the first position
  • FIG. 16B shows the case where the collection substrate 200 is in the second position.
  • the control device 10 controls the drive unit 5 to set the collection substrate 200 at the second position. At this time, the arm of the drive unit 5 comes into contact with the lower end of the opening 1 ⁇ / b> A and inserts the collection substrate 200 into the housing 1.
  • the control device 10 slides the fixture 63 downward from above the opening 1A when the collection substrate 200 is in the second position, and covers the opening 1A with the arm of the drive unit 5 interposed therebetween. Furthermore, the control device 10 can increase the fluid resistance of the opening 1A due to the negative pressure generated in the housing 1 by driving the fan 3 in this state.
  • rubber-like members are provided at the end of the opening 1 ⁇ / b> A in contact with the arm of the drive unit 5 and the end of the fixture 63. Thereby, the fluid resistance of the opening 1A can be further increased.
  • the fixing tool 61 or the fixing tool 63 covers the opening 1 ⁇ / b> A in a state where the arm of the driving unit 5 supports the holding unit 4 and the holding unit 4 holds the collection substrate 200.
  • the arm of the drive unit 5 may return to the outside of the housing 1.
  • FIG. 17 is a diagram for explaining another example of the drive unit 5.
  • FIG. 17A shows a state in which the collection substrate 200 is in the first position
  • FIG. 17B shows a state in which the collection substrate 200 is in the second position
  • FIG. 17C shows the drive unit.
  • 5 represents a state in which only the holding portion 4A is moved out of the housing 1 with the collection substrate 200 in the second position.
  • the collection substrate 200 is detachable from the holding unit 4A
  • the holding unit 4A is also detachable from the drive unit 5.
  • a mounting table 4B for setting the collection substrate 200 is provided in the housing 1.
  • a sandwiching mechanism, a vacuum suction mechanism, an electromagnet mechanism, or the like is preferably used as a configuration for making the collection substrate 200 detachable from the holding portion 4A.
  • the holding unit 4 ⁇ / b> A is electrically connected to the control device 10, and attachment / detachment of the collection substrate 200 is controlled by a control signal from the control device 10.
  • the collection substrate 200 Since the collection device 100 according to the first embodiment has the above-described configuration, the collection substrate 200 is placed while securing a mechanism for collecting particles in the air using the inertial collision method. It can be easily carried in and out of the body 1.
  • the detection device includes the collection device 100 according to the first embodiment, and detects particles in the air collected on the surface of the collection substrate 200.
  • FIG. 18 is a schematic diagram illustrating a specific example of the configuration of the detection apparatus 500 according to the second embodiment.
  • the detection device 500 includes the collection device 100 according to the first embodiment and a detector 300 for detecting particles collected on the surface of the collection substrate 200.
  • the collection device 100, the drive unit 5, and the detector 300 are all electrically connected to the control device 10 and controlled by the control device 10.
  • the drive unit 5 controls the control device 10 so that the collection substrate 200 is set to the second position in the housing 1 of the collection device 100 and then the third position which is a predetermined position in the detector 300. Move to position.
  • the direction of the arm supporting the collection substrate 200 is defined as a first axis direction
  • the direction in which the arm is moved up and down is defined as a second axis direction
  • the direction in which the arm is moved back and forth is defined as a third axis direction.
  • the collection device 100 and the detector 300 are arranged side by side in the third axis direction.
  • the housing 1 and the detector 300 of the collection device 100 each have an opening in the first axial direction.
  • the second axial direction is used for positioning the collection substrate 200 in the housing 1 and the detector 300 of the collection device 100.
  • the detection method of the detector 300 is not limited to a specific method, and a method of detecting scattered light derived from particles, a method of detecting fluorescence derived from particles, or acquiring a particle image and performing the image recognition processing. Detection by the above can be suitably employed.
  • FIG. 19 is a schematic diagram showing a specific example of the configuration of the detector 300.
  • the detector 300 includes a light source 31 for irradiating the surface of the collection substrate 200 and a light receiving element 32 for receiving light from the surface of the collection substrate 200.
  • the light receiving element 32 is electrically connected to the control device 10 and inputs a detection signal indicating the amount of received light to the control device 10.
  • the control device 10 calculates the particle amount based on the received light amount.
  • the light receiving element 32 corresponds to a photodiode or a photomultiplier tube for measuring scattered light from particles on the surface of the collection substrate 200.
  • the light receiving element 32 may be arranged at any angle with respect to the irradiation direction of the light source 31.
  • the light receiving element 32 detects forward scatter, side scatter, back scatter, and the like according to the arranged position.
  • the control device 10 that has received the detection signal from the light receiving element 32 calculates the particle diameter, the complexity of the configuration within the particle, and the like, and compares it with a reference value that is stored in advance, so that the target particle (for example, biological origin) Particles). Or the control apparatus 10 should just count the signal pulse of scattered light, when confirming the presence or absence and number of particle
  • the light source 31 corresponds to one that can irradiate light as excitation light
  • the light receiving element 32 is a photodiode, a photomultiplier tube, a CCD (Charge Coupled Device) for measuring fluorescence from particles.
  • Image sensors such as image sensors and CMOS (Complementary Metal Oxide Semiconductor) image sensors are applicable.
  • the detector 300 may be provided with a fluorescence detection lens 34 and a fluorescence detection filter 35 such as a band pass filter and a long pass filter as necessary.
  • a light source lens 33 for adjusting the irradiation direction may be disposed in front of the irradiation direction of the light source 31.
  • a mirror 36 for adjusting an optical path such as a dichroic mirror may be arranged depending on the angle at which the light receiving element 32 is arranged.
  • the light receiving element 32 corresponds to an image sensor such as a CCD image sensor or a CMOS image sensor for measuring (photographing) a particle image on the surface of the collection substrate 200.
  • the control device 10 that has received the particle image from the light receiving element 32 detects the target particle (for example, biological particles) and the presence / absence of the particle by comparing with a previously stored particle image.
  • the control device 10 controls the drive unit 5 to drive the fan 3 for a predetermined time as the second position in the housing 1 of the collection device 100 to perform the collection operation, and then performs the collection operation. Then, the drive unit 5 is controlled to move the collection substrate 200 to the detector 300.
  • the control device 10 controls the drive unit 5 so that the irradiation range of the light source 31 in the detector 300 and the light receiving element 32
  • the collection substrate 200 is moved to the third position that is the light receiving range. In this state, the control device 10 emits light from the light source 31 and detects particles on the collection substrate 200 based on a detection signal from the light receiving element 32.
  • the detection device 500 according to the second embodiment has the above-described configuration, the collection operation and the detection operation can be performed as a series of operations by one device. Moreover, since the drive part 5 which is a drive means is controlled by the control apparatus 10, as a result, the movement of the collection board
  • FIG. 20 is a particle image photographed by the detection apparatus 500 when air mixed with polystyrene particles is used as a specimen
  • FIG. 21 is photographed by the detection apparatus 500 when air mixed with fungi is used as a specimen.
  • Particle image In any operation for detecting any specimen, first, the collection substrate 200 is set in the housing 1 of the collection apparatus 100 to collect particles in the specimen on the surface, and then the drive unit 5 The collection substrate 200 is conveyed to the detector 300, and a particle image is taken by the detector 300. The number of particles can be calculated by performing image analysis or the like in the control device 10 on the particle image obtained by the detector 300. As shown in these drawings, the detection apparatus 500 according to the present embodiment can detect particles in a fluid with high accuracy.
  • FIG. 22 is a schematic diagram illustrating a specific example of the configuration of the detection apparatus 500 according to the third embodiment.
  • a detection device 500 according to the third embodiment includes the collection device 100 according to the first embodiment, the detector 300 described in the second embodiment, and a heater 400. .
  • the collection device 100, the drive unit 5, the detector 300, and the heater 400 are all electrically connected to the control device 10 and controlled by the control device 10.
  • the drive unit 5 moves the collection substrate 200 to the second position in the housing 1 of the collection device 100 by the control of the control device 10, passes through the heater 400, and at a predetermined position in the detector 300. Move to a third position.
  • Some non-living particles such as chemical fiber dust floating in the air, emit fluorescent light when irradiated with ultraviolet light or blue light, similar to biological particles.
  • the fluorescence intensity of biological particles increases by heating, whereas the fluorescence intensity of non-biological particles such as chemical fiber dust does not change by heating.
  • the detection apparatus 500 concerning 3rd Embodiment detects the particle
  • FIG. 23 shows the measurement results of the fluorescence spectrum of E. coli, which is a biological particle.
  • a curve 71 represents a spectrum before the heat treatment
  • a curve 72 is a spectrum after the heat treatment at 200 ° C. for 5 minutes.
  • FIG. 24A is a fluorescence micrograph before the heat treatment
  • FIG. 24B is a fluorescence micrograph after the heat treatment. From the measurement results shown in FIG. 23 and the comparison between (A) and (B) in FIG. 24, it can be seen that the fluorescence intensity from E. coli is greatly increased by the heat treatment.
  • FIG. 25 shows the measurement results of the fluorescence spectrum of Bacillus bacteria, which are biologically derived particles.
  • a curve 73 represents a spectrum before the heat treatment
  • a curve 73 is a spectrum after the heat treatment at 200 ° C. for 5 minutes.
  • FIG. 26A is a fluorescence micrograph before heat treatment
  • FIG. 26B is a fluorescence micrograph after heat treatment. From the measurement results shown in FIG. 25 and the comparison between (A) and (B) in FIG. 26, it can be seen that the fluorescence intensity from Bacillus bacteria is greatly increased by the heat treatment.
  • FIG. 27 shows the measurement results of the fluorescence spectrum of mold fungi, which are biologically derived particles.
  • a curve 75 represents a spectrum before the heat treatment
  • a curve 76 is a spectrum after the heat treatment at 200 ° C. for 5 minutes.
  • FIG. 28A is a fluorescence micrograph before heat treatment
  • FIG. 28B is a fluorescence micrograph after heat treatment. From the measurement results shown in FIG. 27 and a comparison between (A) and (B) in FIG. 28, it can be seen that the fluorescence intensity from the mold is significantly increased by the heat treatment.
  • FIG. 29 shows the measurement result of the fluorescence spectrum of the dust that emits fluorescence.
  • a curve 77 represents a spectrum before the heat treatment
  • a curve 78 is a spectrum after the heat treatment at 200 ° C. for 5 minutes.
  • FIG. 30A is a fluorescence micrograph before heat treatment
  • FIG. 30B is a fluorescence micrograph after heat treatment.
  • the curve 77 and the curve 78 substantially overlap. That is, the comparison in FIG. 29 and the comparison between FIGS. 30A and 30B show that the fluorescence intensity from dust does not change before and after the heat treatment.
  • control device 10 collects particles with the collection device 100, heats the collection substrate 200 with the heater 400 for a predetermined time, and heats the surface of the collection substrate 200 after being heated with the detector 300. Measure particles (take a particle image). Therefore, the control apparatus 10 can detect the particle
  • the control device 10 collects particles with the collection device 100, moves the collection substrate 200 to the detector 300, and measures particles (takes a particle image) on the surface of the collection substrate 200 before heating. Thereafter, the collection substrate 200 is moved to the heater 400, and the collection substrate 200 is heated by the heater 400 for a predetermined time. Then, the collection board
  • the detection apparatus 500 according to the third embodiment has the above-described configuration, it does not require treatment with a fluorescent staining reagent or the like depending on the fluorescence intensity after heating or the difference in fluorescence intensity before and after heating. Particles can be separated from non-living particles and detected with high accuracy.
  • the control unit 10 controls the driving unit 5 which is driving means, and as a result, the movement of the collection substrate 200 can be controlled, a series of operations of collection, heating, and detection can be easily automated. be able to.

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Abstract

La présente invention concerne un dispositif de collecte (100) qui collecte des particules dans un fluide introduit dans un boîtier (1) sur la surface d'une plaque de collecte (200) installée à l'intérieur du boîtier. Une plaque de buses (2) est placée sur la surface supérieure du boîtier, et l'entraînement d'un ventilateur (3) amène l'air extérieur à être introduit dans le boîtier à un débit prescrit par l'intermédiaire de buses (2A) formées par découpe de trous dans la plaque de buses. Une unité d'entraînement (5) amène la plaque de collecte à se déplacer, à travers une ouverture (1A), entre une première position qui est à l'extérieur du boîtier et une deuxième position qui est à l'intérieur du boîtier et directement en face de la direction dans laquelle les buses ont été formées par la découpe de trous. Le dispositif de collecte est pourvu d'un outil de fixation (61) pour amener la résistance au fluide traversant l'ouverture à être supérieure à la résistance au fluide traversant la buse lorsque la plaque de collecte est dans la deuxième position.
PCT/JP2014/069888 2013-08-30 2014-07-29 Dispositif de collecte et dispositif de détection Ceased WO2015029673A1 (fr)

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EP3869178A1 (fr) * 2020-01-21 2021-08-25 Honeywell International Inc. Dispositif de capteur de composition de fluide et son procédé d'utilisation
US11181456B2 (en) 2020-02-14 2021-11-23 Honeywell International Inc. Fluid composition sensor device and method of using the same
JP2022505288A (ja) * 2018-11-16 2022-01-14 パーティクル・メージャーリング・システムズ・インコーポレーテッド ロボット制御製造バリアシステムのための粒子サンプリングシステム及び方法
US11391613B2 (en) 2020-02-14 2022-07-19 Honeywell International Inc. Fluid composition sensor device and method of using the same
US11835432B2 (en) 2020-10-26 2023-12-05 Honeywell International Inc. Fluid composition sensor device and method of using the same
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US12281976B2 (en) 2021-05-13 2025-04-22 Honeywell International Inc. In situ fluid sampling device and method of using the same
US12292362B2 (en) 2019-04-26 2025-05-06 Honeywell International Inc. Flow device and associated method and system

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US11923081B2 (en) 2017-09-27 2024-03-05 Honeywell International Inc. Respiration-vocalization data collection system for air quality determination
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US12292362B2 (en) 2019-04-26 2025-05-06 Honeywell International Inc. Flow device and associated method and system
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US12181400B2 (en) 2020-02-14 2024-12-31 Honeywell International Inc. Fluid composition sensor device and method of using the same
US11181456B2 (en) 2020-02-14 2021-11-23 Honeywell International Inc. Fluid composition sensor device and method of using the same
US12111257B2 (en) 2020-08-26 2024-10-08 Honeywell International Inc. Fluid composition sensor device and method of using the same
US12209941B2 (en) 2020-10-26 2025-01-28 Honeywell International Inc. Fluid composition sensor device and method of using the same
US11835432B2 (en) 2020-10-26 2023-12-05 Honeywell International Inc. Fluid composition sensor device and method of using the same
US12281976B2 (en) 2021-05-13 2025-04-22 Honeywell International Inc. In situ fluid sampling device and method of using the same
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