WO2015027923A1 - Electroporation system based on microelectrode chip - Google Patents

Abstract

Disclosed is an electroporation system based on a microelectrode chip, comprising: a pulse generator module and an electroporation carrier, said module and carrier being electrically connected. The pulse generator module comprises a power supply circuit unit, a control module circuit unit, a touch input circuit unit and a voltage amplification circuit unit, said units being electrically connected. The control module circuit unit is used to control, according to inputted information, the voltage amplification circuit unit to output a required pulse signal to the electroporation carrier, thus performing electroporation on the microelectrode chip within the electroporation carrier. The power supply circuit unit is used to supply power to the touch input circuit unit, the control module circuit unit and the voltage amplification circuit unit. The present electroporation system based on the microelectrode chip provides customized requirements for the microelectrode, and is effective, free from chemical pollution and highly efficient. The waveform generated by the pulse generator module is agile.

Description

 Electroporation system based on microelectrode chip

Technical field

The present invention relates to the field of electroporation of cell membranes, and more particularly to an electroporation system based on microelectrode chips.

Background technique

Studies have found that if a certain intensity of electrical stimulation is applied to the cells for a period of time, it can induce some micropores in the cell membrane to enhance the permeability of the cells. Electroporation refers to the application of a pulsed electric field to the cells. Under the action, the biophysical process of forming transient micropores on the cell membrane lipid bilayer. When the cell membrane undergoes electroporation, its permeability and membrane conductance increase instantaneously, allowing hydrophilic molecules, DNA, proteins, viral particles, drug particles, and the like, which normally cannot pass through the cell membrane, to enter the cell. After the electric field is removed in a short time, the cell membrane can self-recover and become a selective permeability barrier. Compared with traditional chemical perforation and virus perforation, electroporation has broad application in biophysics, molecular biology, clinical medicine and other fields because it has no chemical pollution, no permanent damage to cells, and high efficiency. prospect.

In the systems disclosed in the prior art, plate electrodes are often used, and the distance between the electrodes is usually about 2-10 mm, which is much larger than the typical scale of the cells (20 micrometers), so it is difficult to precisely control the electric field actually applied to the cells. . At the same time, in the electric field generated by the two plate electrodes, the electric field E=V/D (V is the voltage applied between the two plates, D is the distance between the two plates), and the distance D between the plates is large. In existing electroporation systems, the required voltage is typically up to several thousand volts, which causes most cells to die during electroporation, increasing the difficulty, time and expense of electroporation.

Due to the emergence of the new microelectrode chip, the spacing of the electrodes is made small, so that the voltage required for electroporation is greatly reduced, which can improve cell survival rate and electroporation efficiency. However, the electroporation instruments on the market are not capable of providing the corresponding interfaces required for the microelectrode chip and the waveforms that can be flexibly converted.

Summary of the invention

The technical problem to be solved by the present invention is to provide a waveform for the microelectrode chip to provide customized requirements, strong versatility, good electroporation effect, no chemical pollution, no damage to cells, high efficiency, and pulse generator module. Flexible electroporation system based on a new microelectrode chip.

In order to solve the above technical problem, the present invention provides an electroporation system based on a novel microelectrode chip, comprising: an electrically connected pulse generator module and an electroporation carrier, the pulse generator module comprising a touch input circuit unit, a power circuit unit, a control module circuit unit, and a voltage amplifying circuit unit, wherein the touch input circuit unit is configured to receive information input by a user and send the information to the control module circuit unit, where the control module circuit unit is configured to use the input And controlling the voltage amplifying circuit unit to output a required pulse signal to the electroporation carrier to discharge the microelectrode chip in the electroporation carrier; the power circuit unit is configured to be the touch The control input circuit unit, the control module circuit unit, and the voltage amplifying circuit unit supply power.

In a preferred embodiment, the pulse generator module further includes an impedance detecting circuit unit for detecting an impedance of the cell solution in the electroporation carrier under the control of the control module circuit unit and returning the detection result to the The control module circuit unit is configured to determine whether to control the voltage amplifying circuit unit to output a pulse signal according to the detection result.

In a preferred embodiment, the pulse generator module further includes at least one of an overcurrent protection circuit unit, an LCD display circuit unit, a memory module circuit unit, and a buzzer circuit unit;

The overcurrent protection circuit unit is configured to perform overcurrent protection when the current in the circuit exceeds the warning value and transmit the overcurrent signal to the control module circuit unit;

The LCD display circuit unit is configured to display corresponding data and status under the control of the control module circuit unit;

The storage module circuit unit is configured to store corresponding data under the control of the control module circuit unit;

The buzzer circuit unit is configured to output a prompt tone under the control of the control module circuit unit.

In a preferred embodiment, the electroporation carrier is a single-hole plate electroporation carrier, and the single-hole plate electroporation carrier has a square box shape, and the single-hole plate electroporation carrier includes an upper cover, a bottom plate, and a rotating shaft for connecting the upper cover and the bottom plate, wherein a side of the upper cover opposite to the bottom plate is provided with a liquid pressure column and a test pin mounting hole for mounting a test pin, the bottom plate and the upper cover a side of the opposite arrangement is provided with a chip mounting groove for mounting the microelectrode chip, the position of the chip mounting groove is matched with the position of the liquid pressure column, and the test pin mounting hole position is mounted with the chip The slot is matched, and one end of the test pin is electrically connected to the pulse generator module, and one end is connected to the electrode of the microelectrode chip.

In a preferred embodiment, the test pin mounting holes are two or four.

In a preferred embodiment, the bottom surface area of the hydrostatic column matches the size of the microelectrode chip.

In a preferred embodiment, the upper cover and the bottom plate are respectively provided with first and second buckles that cooperate with each other.

In a preferred embodiment, the electroporation carrier is a perforated plate electroporation chip cassette;

The microelectrode chip-based electroporation system further includes a relay control module electrically connected to the pulse generator module at one end and electrically connected to the electroporation carrier at the other end;

The pulse generator module further includes a communication module unit, wherein the control module circuit unit communicates with the relay control module through the communication module unit, and controls on and off of the corresponding relay to select the corresponding one of the at least one board hole The microelectrode chip is discharged.

In a preferred embodiment, the impedance detecting circuit unit is configured to detect impedance of a cell solution in all the plate holes and return the detection result to the control module circuit unit;

The control module circuit unit is configured to control the on/off of the corresponding relay according to the detection result to select the microelectrode chip in the corresponding at least one plate hole for discharging.

In a preferred embodiment, the set of pulses of the pulse generator module consists of a high pulse and a low pulse.

The high pulse includes at least 5 high pulses, each of which is characterized by two parameters, a high pulse width and a high pulse height.

Wherein the high pulse width L=1-9999μs,

The high pulse height P=0-600V, the minimum adjustment precision is 1V;

The low pulse is characterized by two parameters, a low pulse height and a low pulse width.

Wherein the low pulse width l=1-9999μs,

The low pulse height is p=0-100V, and the minimum adjustment precision is 1V.

The pulse generator module has a pulse group number of 1-9999, and the interval between each group of pulses is 1-9999 ms.

The beneficial effects of the invention are:

The electroporation system based on the microelectrode chip provides customized requirements for the microelectrode chip, has strong versatility, good electroporation effect, no chemical pollution, no damage to cells, high efficiency, and flexible waveform generated by the pulse generator module. .

DRAWINGS

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained according to these drawings without any creative work, wherein:

1 is a schematic flow chart of Embodiment 1 of a microelectrode chip-based electroporation system of the present invention;

2 is a schematic flow chart of a pulse generator module in Embodiment 1 of a microelectrode chip-based electroporation system of the present invention;

3 is a schematic structural view of a single-hole electroporation vehicle in the microelectrode chip-based electroporation system according to Embodiment 1 of the present invention;

4 is a schematic structural view of a perforated plate electroporation carrier in Embodiment 2 of the microelectrode chip-based electroporation system of the present invention;

The components of the drawing are marked as follows: 1. pulse generator module; 11, power circuit unit; 12, control module circuit unit; 13, touch input circuit unit; 14, overcurrent protection circuit unit; 15, LCD display circuit Unit; 16, memory module circuit unit; 17, voltage amplifying circuit unit; 18, buzzer circuit unit; 19, impedance detecting circuit unit; 20, communication module unit; 2, relay control module; 3, electroporation vehicle; 311, rotating shaft; 312, liquid pressure column; 313, test pin mounting hole; 314, first buckle; 315, chip mounting groove; 316, second buckle; 32, perforated plate electroporation chip box.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present invention without creative efforts are within the scope of the present invention.

Example 1:

Referring to FIG. 1 and FIG. 2, an electroporation system based on a microelectrode chip includes: an electrically connected pulse generator module 1 and an electroporation carrier 3. The pulse generator module 1 includes a power supply circuit unit 11 and a control module circuit unit 12 electrically connected thereto. The pulse generator module 1 further includes a touch input circuit unit 13 and an overcurrent protection circuit electrically connected to the control module circuit unit 12. The unit 14, the LCD display circuit unit 15, the memory module circuit unit 16, the voltage amplifying circuit unit 17, the buzzer circuit unit 18, and the impedance detecting circuit unit 19. The touch input circuit unit 13, the overcurrent protection circuit unit 14 and the impedance detecting circuit unit 19 input signals to the control module circuit unit 12, and the control module circuit unit 12 outputs signals to the LCD display circuit unit 15, the memory module circuit unit 16, The voltage amplifying circuit unit 17, the buzzer circuit unit 18, and the impedance detecting circuit unit 19.

The pulse generator module 1 operates in a manner that the control module circuit unit 12 receives data from the touch input circuit unit 13, controls the LCD display circuit unit 15 to display data and controls the voltage amplification circuit unit 17 to generate the required electrical pulse, when the current in the circuit When the warning value is exceeded, the overcurrent protection circuit unit 14 transmits an error signal to the control module circuit unit 12, and the control module circuit unit 12 controls the voltage amplification circuit unit 17 to stop operating, and the storage module circuit unit 16 receives the signal of the control module circuit unit 12. The user data is stored, and the buzzer circuit unit 18 receives the command of the control module circuit unit 12 to generate various warnings or reminders. When the voltage amplifying circuit unit 17 completes the output of the electrical pulse, the electroporation process ends.

The control module circuit unit 12 in the pulse generator module 1 is used for control of the entire electroporation system, including receiving and responding to the data of the touch input circuit unit 13, controlling the buzzer circuit unit 18 to generate various warnings or reminders, and controlling The LCD display circuit unit 15 instantly displays data and status, and the control voltage amplifying circuit unit 17 outputs the required electric pulse and receives and feeds back the data of the impedance detecting circuit unit 19.

The touch input circuit unit 13 in the pulse generator module 1 includes a resistive touch screen and an AD conversion circuit. The control module circuit unit 12 receives data from the AD conversion circuit for analyzing the touch position and performs a corresponding operation.

The LCD display circuit unit 15 in the pulse generator module 1 includes a digital circuit chip and a liquid crystal panel. The control module circuit unit 12 controls the switches of the digital circuit chip. The control chip in the LCD screen accepts external data, controls the LED backlight and pixels to display the corresponding content.

The memory module circuit unit 16 in the pulse generator module 1 is used to store user electroporation parameters. The storage module circuit unit 16 stores the corresponding data when receiving the instruction from the control module circuit unit 12.

The voltage amplifying circuit unit 17 in the pulse generator module 1 is a key part of the electroporation pulse generator. The voltage amplifying circuit unit 17 includes a first-stage amplifying circuit, a second-stage amplifying circuit, and a power amplifying module. The primary amplification circuit receives control from the control module circuit unit 12. After the output of the first stage amplifier. Then through the secondary amplification module, the output voltage of 0-+600V is obtained.

The buzzer circuit unit 18 is configured to prompt a button sound and a program prompt sound, and the switch module circuit unit 12 applies different levels to the triode to control the buzzer switch, and the buzzer circuit unit 18 is composed of a triode and The buzzer is composed of a button sound prompt and an alarm for the pulse generator module 1.

In order to avoid a short circuit or a cell solution in the microelectrode chip, the impedance detecting circuit unit 19 in the pulse generator module 1 of the present application is configured to detect the cell solution in the microelectrode chip according to the instruction of the control module circuit unit 12. The size of the impedance. The impedance detecting circuit unit 19 includes an impedance detecting module, an amplifier, and a sampling circuit. The impedance detection module passes through the amplifier and sampling circuit and then undergoes Fourier transform to return the real and imaginary parts of the impedance. The impedance detecting circuit unit 19 then returns the detection result to the control module circuit unit 12, so that the control module circuit unit 12 decides whether or not to stop the corresponding operation. For example, when it is found that a conductive substance exists in the cell solution based on the detection result, the control module circuit unit 12 outputs a control signal for stopping the discharge.

The power supply circuit unit 11 in the pulse generator module 1 is used to supply the voltage required for each module. The power supply circuit unit 11 includes a voltage stabilization chip and a switching power supply. The switching power supply in the power circuit unit 11 has a short circuit protection circuit, which can prevent the circuit load from being short-circuited and affecting the circuit.

The respective control circuits are connected by a digital-analog circuit internal bus mode, and the buses include digital signal lines and analog signal lines, so that the signal transmission is faster and the bit error rate is lower.

As shown in FIG. 3, the electroporation carrier 3 is a single-hole plate electroporation carrier, and the single-hole plate electroporation carrier is arranged in a square box shape, which comprises a box upper cover, a bottom plate and a top cover and a bottom plate connecting the box. a rotating shaft 311, a side of the upper cover of the box opposite to the bottom plate is provided with a liquid pressure column 312, a test pin mounting hole 313 for mounting the test pin, and a first buckle 314, and the opposite side of the bottom plate and the upper cover is provided for The chip mounting groove 315 and the second buckle 316 of the single-hole microelectrode chip are mounted, and the first buckle 314 and the second buckle 316 cooperate to lock. The tank 315 is mated with the pressure liquid column 312. The test pin in the electroporation carrier 3 is used for electrically connecting the single-hole microelectrode chip of the electroporation carrier 3 to the pulse generator module 1 to pulse the pulse amplifying circuit unit 17 in the pulse generator module 1. Transfer to a single well microelectrode chip. The position of the test pin is identical to the position of the single-electrode microelectrode chip electrode, and the size of the test pin can be selected to match the size of the test pin mounting hole 313. The above microelectrode chip may refer to "an electroporation chip and a perforated plate device based on an electroporation chip" Patent No. 200910237334.X The ring-shaped electrode chip in the middle.

In an alternative embodiment, the test pin mounting holes 313 are four, and the test pin mounting holes 313 are positioned to match the position of the chip mounting slot 315. The test pin is mounted in the test pin mounting hole 313. When the electroporation carrier 3 is in the closed state, the test pin is connected to the electrode of the single-hole microelectrode chip, and the pulse generator module 1 is provided by electroporating the single-hole microelectrode chip. Electrical connection.

In addition, the area of the bottom surface of the liquid pressure column 312 matches the size of the single orifice microelectrode chip. The electroporation carrier 3 is closed and placed in the slot of the pulse generator module 1, releasing the pulse to complete the electroporation operation. The pressure liquid column 312 referred to in FIG. 3 is just above the single-hole microelectrode chip when the electroporation carrier 3 is closed, and the distance from the micro-electrode chip of the single-hole plate is 10 μm - 1 mm, in the first embodiment When the electroporation carrier 3 is closed, the distance between the liquid pressure column 312 and the underlying single-hole microelectrode chip is 10 μm, and the height of the liquid pressure column 312 matches the liquid level of the surface of the single-hole microelectrode chip.

In the present application, a set of waveforms of the pulse generator module 1 may be composed of a high pulse and a low pulse.

The single high pulse can be continuously combined by 5 or more small pulses, each of which is characterized by two parameters: high pulse width and high pulse height. These different parameters can be combined into high pulse of different shapes.

Among them, the high pulse width L=1-9999μs,

High pulse height P=0-4600V, minimum adjustment accuracy is 1V.

The low pulse can be characterized by two parameters: low pulse height and low pulse width.

Among them, the low pulse width l=1-9999μs,

Low pulse height p=0-100V, minimum adjustment accuracy is 1V,

The pulse generator module 1 can have a pulse group number of 1-9999, and the interval between each group of pulses can be 1-9999 ms. The above pulse can be simulated by the LCD display circuit unit.

In the first embodiment of the present application, the user can preset a series of pulse parameters including the above content through the touch input circuit unit 13 as needed, so that the control module circuit unit 12 controls the voltage amplifying circuit unit 17 to output a customized pulse waveform.

In the first embodiment, a set of waveforms of the pulse generator module 1 is composed of a high pulse and a low pulse.

The preset high pulse includes 3 levels, each stage is characterized by two parameters: high pulse height and high pulse width, respectively, 250V, 20μs; 200V, 50μs; 180V, 20μs,

The low pulse has a low pulse width of l=100 μs,

Low pulse height p=1V,

The number of pulse groups of the pulse generator module 1 is 20, and the interval time between each group of pulses is 2 ms, and the pulse is simulated by the LCD display circuit unit.

Example 2:

Different from Embodiment 1, as shown in FIG. 4, the electroporation carrier 3 is a perforated plate electroporation chip cassette 32. As shown in FIG. 1, the microelectrode chip based electroporation system further includes an end and a pulse generator module. A relay control module 2 electrically connected and electrically connected to the electroporation carrier 3 at the other end.

As shown in FIG. 1, the pulse generator module 1 includes a power supply circuit unit 11 and a control module circuit unit 12 electrically connected thereto. The pulse generator module 1 further includes a touch input circuit unit 13 electrically connected to the control module circuit unit 12, an overcurrent protection circuit unit 14, an LCD display circuit unit 15, a memory module circuit unit 16, a voltage amplifying circuit unit 17, and a bee. The sounder circuit unit 18, the impedance detecting circuit unit 19, and the communication module unit 20. The touch input circuit unit 13, the overcurrent protection circuit unit 14, the impedance detecting circuit unit 19, and the communication module unit 20 input signals to the control module circuit unit 12, and the control module circuit unit 12 outputs signals to the LCD display circuit unit 15, the memory module circuit. The unit 16, the voltage amplifying circuit unit 17, the buzzer circuit unit 18, the impedance detecting circuit unit 19, and the communication module unit 20.

The perforated plate electroporation chip cassette 32 is electrically connected to the relay control module 2. The perforated plate electroporation chip cassette 32 has a thimble and a spring device (not shown) capable of contacting the electrodes of the perforated plate of the perforated plate and a fixed perforated plate, and has a pulse between the pulse generator module 1 and the pulse generator module 1. A signal interface for transmitting the pulse signal of the voltage amplifying circuit unit 17 in the pulse generator module 1 to the chip in the perforated chip box 32 of the perforated plate. The communication module unit 20 of the pulse generator module 1 is used to communicate with the relay control module 2. The communication module unit 20 is specifically a communication interface for selecting a multi-plate hole, which is a serial port or an RS485 interface, and the pulse generator module 1 controls the switch of the relay in the relay control module 2 through the serial port or the RS485 interface to select The microelectrode chips in the corresponding multi-plate holes are electroporated. The relay control module 2 is specifically a digital intelligent instrument composed of a single-chip microcomputer as a core and a corresponding hardware circuit with a special application program.

In order to avoid a short circuit or a case where there is no cell solution in the selected microelectrode chip, the impedance detecting circuit unit 19 in the pulse generator module 1 of the present application is configured to detect the microscopy corresponding to all the plate holes according to the instruction of the control module circuit unit 12. The impedance of the cell solution in the electrode chip is returned to the control module circuit unit 12, and the control module circuit unit 12 controls the on and off of the relay in the relay control module 2 according to the detection result, and selects the microelectrode chip in the corresponding plate hole. Electroporation.

For example, according to the parameters input by the user, the microelectrode chip in the hole of the sixth row and the sixth column needs to be electroporated, but the detection by the impedance detecting circuit unit 19 finds that there is no cell solution, but on the seventh line. There is a cell solution in the microelectrode chip in the 6-row plate hole. At this time, the control module circuit unit 12 adjusts the on-off of the relay to discharge the microelectrode chip in the hole of the sixth row and the sixth column.

The electroporation system based on the microelectrode chip of the invention has a total of 8 working modes:

1. Manual electroporation mode:

The user inputs the electroporation parameters through the touch input circuit unit 13, and the control module circuit unit 12 controls the voltage amplifying circuit unit 17 to emit a corresponding pulse signal to electroporate the single-hole microelectrode chip.

2. Intelligent electroporation mode:

The user uses the cell library that is provided by the system, and selects corresponding cells through the touch input circuit unit 13, and the control module circuit unit 12 controls the voltage amplifying circuit unit 17 to emit a corresponding pulse signal to electroporate the single-hole microelectrode chip.

3. Bacterial electroporation mode:

The user uses the system to bring the bacteria library, and selects the corresponding bacteria type through the touch input circuit unit 13, and the control module circuit unit 12 controls the voltage amplifying circuit unit 17 to emit a corresponding pulse signal to electroporate the single-hole microelectrode chip.

4. Cell fusion.

The user pre-processes the cells on the chip and selects the cell fusion mode through the touch input circuit unit 13 to fuse the cells. The control module circuit unit 12 controls the voltage amplifying circuit unit 17 to emit a corresponding pulse signal to electroporate the single-hole microelectrode chip.

5. Multi-well plate mode:

The user can select a corresponding perforated plate through the touch input circuit unit 13, and the control module circuit unit 12 sends an instruction to the voltage amplifying circuit unit 17 and the relay control module 2 to select a corresponding plate hole, sends a pulse signal, and simultaneously sends an instruction to the impedance detecting circuit. Unit 19, the control module circuit unit 12 replaces the selected electroporated plate holes according to the feedback of the impedance detecting circuit unit 19, and emits a pulse signal, thus alternating.

6. Impedance measurement mode;

Used to measure the impedance of cells on a chip.

7. Cell lysis mode:

The voltage amplifying circuit unit 17 releases a relatively long high-voltage pulse, so that the cell membrane can not be recovered by rupture, thereby achieving the purpose of cell lysis.

8. Waveform conversion mode:

The pulse waveforms emitted by the voltage amplifying circuit unit 17 can be changed and combined as needed to explore waveform data suitable for electroporation. In the waveform transform mode, a set of waveforms is composed of high pulses and low pulses, and high pulses can be divided into 5 levels or more. Each stage is characterized by two parameters: high pulse width and high pulse height. The high pulse width is L=1-9999μs, the high pulse height is P=0-600V, the minimum adjustment precision is 1V, and the low pulse is caused by low pulse height and low pulse. Width characterization. Low pulse width l=1-9999μs, low pulse height p=0-100V, minimum adjustment accuracy is 1V, pulse group number: 1-9999 groups, the interval between each group of pulses is 1-9999ms, each group of pulses can The display is simulated by the display.

In the second embodiment, a set of waveforms in the waveform conversion mode is composed of a high pulse and a low pulse, and the high pulse can be divided into five levels, each stage being characterized by two parameters of a high pulse width and a high pulse height, and the high pulse width L is in turn 9999μs, 8888μs, 7777μs, 6666μs, 5555μs, high pulse height P in order of 400V, 350 V, 300 V, 250 V, 200 V, the minimum adjustment accuracy is 1V. The low pulse has a low pulse width of l=9999μs, a low pulse height of p=100V, and a minimum adjustment accuracy of 1V. The number of pulse groups is 9999 groups, and the interval between each group of pulses is 9999ms. The pulse of each group can be simulated by the display.

Different from the prior art, the electroporation system based on the novel microelectrode chip provides customized requirements for the microelectrode, has strong versatility, good electroporation effect, no chemical pollution, no damage to cells, high efficiency, and pulse generation. The waveform generated by the module is flexible.

The above is only the embodiment of the present invention, and thus does not limit the scope of the patent of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification of the present invention, or directly or indirectly applied to other related technical fields, The same is included in the scope of patent protection of the present invention.

Claims (10)

What is claimed is: 1. An electroporation system based on a microelectrode chip, comprising: an electrically connected pulse generator module and an electroporation carrier, the pulse generator module comprising a touch input circuit unit, a power supply circuit unit, a control module circuit unit and a voltage amplifying circuit unit, wherein the touch input circuit unit is configured to receive information input by a user and send the information to the control module circuit unit, where the control module circuit unit is configured to control the information according to the input information The voltage amplifying circuit unit outputs a required pulse signal to the electroporation carrier, and discharges the microelectrode chip in the electroporation carrier; the power circuit unit is used for the touch input circuit unit And the control module circuit unit and the voltage amplifying circuit unit supply power. 2. The microelectrode chip based electroporation system according to claim 1, wherein the pulse generator module further comprises an impedance detecting circuit unit for detecting the under the control of the control module circuit unit Electrolyzing the impedance of the cell solution in the carrier and returning the detection result to the control module circuit unit; the control module circuit unit is configured to determine whether to control the voltage amplifying circuit unit to output a pulse signal according to the detection result. 3. The microelectrode chip-based electroporation system according to claim 1, wherein the pulse generator module further comprises an overcurrent protection circuit unit, an LCD display circuit unit, a memory module circuit unit, and a buzzer circuit. At least one unit in the unit; The overcurrent protection circuit unit is configured to perform overcurrent protection when the current in the circuit exceeds the warning value and transmit the overcurrent signal to the control module circuit unit; The LCD display circuit unit is configured to display corresponding data and status under the control of the control module circuit unit; The storage module circuit unit is configured to store corresponding data under the control of the control module circuit unit; The buzzer circuit unit is configured to output a prompt tone under the control of the control module circuit unit. The microelectrode chip-based electroporation system according to claim 1, wherein the electroporation carrier is a single-hole plate electroporation carrier, and the single-hole plate electroporation carrier has a square box shape. The single-hole plate electroporation carrier includes an upper cover, a bottom plate, and a rotating shaft for connecting the upper cover and the bottom plate, and a side of the upper cover opposite to the bottom plate is provided with a liquid pressure column and is installed a test pin mounting hole of the test pin, a side of the bottom plate opposite to the upper cover is provided with a chip mounting groove for mounting the microelectrode chip, a position of the chip mounting groove and a position of the liquid pressure column The test pin mounting hole is matched with the position of the chip mounting slot, and one end of the test pin is electrically connected to the voltage amplifying circuit unit, and one end is connected to the electrode of the microelectrode chip. 5. The microelectrode chip based electroporation system according to claim 4, wherein the test pin mounting holes are two or four. 6. The microelectrode chip based electroporation system of claim 4, wherein a bottom surface area of the hydrostatic column matches a size of the microelectrode chip. The microelectrode chip-based electroporation system according to claim 4, wherein the upper cover and the bottom plate are respectively provided with a first buckle and a second buckle that cooperate with each other. The microelectrode chip-based electroporation system according to claim 2, wherein the electroporation carrier is a perforated plate electroporation chip cartridge; The microelectrode chip-based electroporation system further includes a relay control module electrically connected to the pulse generator module at one end and electrically connected to the electroporation carrier at the other end; The pulse generator module further includes a communication module unit, wherein the control module circuit unit communicates with the relay control module through the communication module unit, and controls on and off of the corresponding relay to select the corresponding one of the at least one board hole The microelectrode chip is discharged. The microelectrode chip-based electroporation system according to claim 8, wherein the impedance detecting circuit unit is configured to detect impedance of a cell solution in all plate holes and return the detection result to the control module. Circuit unit The control module circuit unit is configured to control the on/off of the corresponding relay according to the detection result to select the microelectrode chip in the corresponding at least one plate hole for discharging. The microelectrode chip-based electroporation system according to any one of claims 1 to 9, wherein a set of pulses of the pulse generator module is composed of a high pulse and a low pulse. The high pulse includes at least 5 high pulses, each of which is characterized by two parameters, a high pulse width and a high pulse height. Wherein the high pulse width L=1-9999μs, The high pulse height P=0-600V, the minimum adjustment precision is 1V; The low pulse is characterized by two parameters, a low pulse height and a low pulse width. Wherein the low pulse width l=1-9999μs, The low pulse height is p=0-100V, and the minimum adjustment precision is 1V. The pulse generator module has a pulse group number of 1-9999, and the interval between each group of pulses is 1-9999 ms.