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WO2015026845A3 - Analogues nucléotidiques - Google Patents

Analogues nucléotidiques Download PDF

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Publication number
WO2015026845A3
WO2015026845A3 PCT/US2014/051726 US2014051726W WO2015026845A3 WO 2015026845 A3 WO2015026845 A3 WO 2015026845A3 US 2014051726 W US2014051726 W US 2014051726W WO 2015026845 A3 WO2015026845 A3 WO 2015026845A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide analogs
nucleotides
manipulation
compositions
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2014/051726
Other languages
English (en)
Other versions
WO2015026845A2 (fr
Inventor
Dae Hyun Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Molecular Inc
Original Assignee
Abbott Molecular Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Molecular Inc filed Critical Abbott Molecular Inc
Priority to CN201480057284.6A priority Critical patent/CN105722850B/zh
Priority to EP14837595.9A priority patent/EP3036248B1/fr
Priority to RU2016107198A priority patent/RU2688435C2/ru
Priority to ES14837595T priority patent/ES2805004T3/es
Priority to CA2921619A priority patent/CA2921619C/fr
Publication of WO2015026845A2 publication Critical patent/WO2015026845A2/fr
Publication of WO2015026845A3 publication Critical patent/WO2015026845A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne une technologie relative à la manipulation et à la détection d'acides nucléiques, y compris, entre autres, des compositions, des méthodes et des trousses associées à des nucléotides comprenant une fraction de liaison chimiquement réactive.
PCT/US2014/051726 2013-08-19 2014-08-19 Analogues nucléotidiques Ceased WO2015026845A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN201480057284.6A CN105722850B (zh) 2013-08-19 2014-08-19 核苷酸类似物
EP14837595.9A EP3036248B1 (fr) 2013-08-19 2014-08-19 Analogues nucléotidiques
RU2016107198A RU2688435C2 (ru) 2013-08-19 2014-08-19 Набор для получения реакционной смеси для синтеза 3′-o-пропаргил-модифицированной нуклеиновой кислоты
ES14837595T ES2805004T3 (es) 2013-08-19 2014-08-19 Análogos de nucleótidos
CA2921619A CA2921619C (fr) 2013-08-19 2014-08-19 Analogues nucleotidiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361867202P 2013-08-19 2013-08-19
US61/867,202 2013-08-19

Publications (2)

Publication Number Publication Date
WO2015026845A2 WO2015026845A2 (fr) 2015-02-26
WO2015026845A3 true WO2015026845A3 (fr) 2015-05-07

Family

ID=52467107

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/051726 Ceased WO2015026845A2 (fr) 2013-08-19 2014-08-19 Analogues nucléotidiques

Country Status (7)

Country Link
US (5) US20150050697A1 (fr)
EP (1) EP3036248B1 (fr)
CN (1) CN105722850B (fr)
CA (2) CA3112661A1 (fr)
ES (1) ES2805004T3 (fr)
RU (1) RU2688435C2 (fr)
WO (1) WO2015026845A2 (fr)

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ES2764096T3 (es) 2013-08-19 2020-06-02 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
WO2015026845A2 (fr) 2013-08-19 2015-02-26 Abbott Molecular Inc. Analogues nucléotidiques
JP6564369B2 (ja) 2013-12-09 2019-08-21 デュレクト コーポレイション 薬学的活性剤複合体、ポリマー複合体、ならびにこれらを伴う組成物及び方法
US20170146517A1 (en) 2015-11-20 2017-05-25 Oregon Health And Science University Compounds and methods used in assessing mono-parp activity
JP7143221B2 (ja) * 2016-04-07 2022-09-28 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー ５－ヒドロキシメチル化無細胞系ｄｎａをシーケンシングすることによる非侵襲性診断
US20170298422A1 (en) 2016-04-18 2017-10-19 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous single-molecule epigenetic imaging of dna methylation and hydroxymethylation
EP3463381A4 (fr) * 2016-05-23 2020-01-01 The Trustees of Columbia University in the City of New York Dérivés nucléotidiques et leurs méthodes d'utilisation
CA3024875A1 (fr) 2016-06-16 2017-12-21 Richard Edward Watts Synthese combinatoire dirigee et enregistree d'oligonucleotides de molecules de sondes codees
EP3538669A4 (fr) * 2016-11-10 2020-05-20 The Scripps Research Institute Codage de bibliothèque de composés en phase solide avec codage à barres à base de polynucléotides
WO2018204420A1 (fr) 2017-05-02 2018-11-08 Haystack Sciences Corporation Molécules de vérification de synthèse combinatoire dirigée par oligonucléotides et procédés de fabrication et d'utilisation associés
CN110678559B (zh) * 2017-08-31 2023-09-15 深圳华大智造科技股份有限公司 一种核酸探针以及一种核酸测序方法
CN110691854B (zh) * 2017-10-25 2023-09-12 深圳华大生命科学研究院 一种核酸测序方法以及一种核酸测序试剂盒
US11841371B2 (en) * 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
EP3547315B1 (fr) * 2018-03-26 2021-01-27 IMEC vzw Procédé de stockage de données
WO2019191532A1 (fr) * 2018-03-30 2019-10-03 Massachusetts Institute Of Technology Séquençage d'arn monocellulaire à l'aide d'une chimie click
WO2019217939A1 (fr) * 2018-05-11 2019-11-14 Complete Genomics, Inc. Polysubstrats et leurs procédés d'utilisation
CN111330022B (zh) * 2018-12-18 2022-09-20 深圳先进技术研究院 肿瘤靶向dna荧光探针及其制备方法和应用
US12169198B2 (en) * 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
CN109970832B (zh) * 2019-04-09 2022-06-03 沈阳药科大学 一种炔基修饰的脱氧腺苷亚磷酰胺单体及其制备方法
CN110041390B (zh) * 2019-04-26 2022-05-27 上海药明康德新药开发有限公司 DNA编码化合物库中On-DNA的1,2,3-三氮唑化合物的合成方法
KR102811825B1 (ko) * 2019-06-21 2025-05-26 써모 피셔 사이언티픽 발틱스 유에이비 차세대 시퀀싱 라이브러리를 제조하기 위한 핵산 표지화에 유용한 올리고뉴클레오티드-테더된 트리포스페이트 뉴클레오티드
US20210024919A1 (en) * 2019-07-22 2021-01-28 Massachusetts Institute Of Technology Functionalized solid support
US20240115500A1 (en) * 2020-12-09 2024-04-11 Aravasc Inc. Polymeric micelle complexes of mrna or zwitterionic agents, and formulations and uses thereof
CN113304274B (zh) * 2021-04-23 2025-03-04 天津全和诚生物技术有限公司 缀合物及其制备方法和用途
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Also Published As

Publication number Publication date
CA2921619A1 (fr) 2015-02-26
US20200157606A1 (en) 2020-05-21
US20180320219A1 (en) 2018-11-08
WO2015026845A2 (fr) 2015-02-26
CN105722850A (zh) 2016-06-29
CA2921619C (fr) 2021-05-25
US10577646B2 (en) 2020-03-03
CA3112661A1 (fr) 2015-02-26
US9932623B2 (en) 2018-04-03
RU2016107198A (ru) 2017-09-26
US20210207192A1 (en) 2021-07-08
RU2688435C2 (ru) 2019-05-21
ES2805004T3 (es) 2021-02-10
US20150051113A1 (en) 2015-02-19
EP3036248A4 (fr) 2017-03-01
EP3036248A2 (fr) 2016-06-29
US10995363B2 (en) 2021-05-04
RU2016107198A3 (fr) 2018-08-01
EP3036248B1 (fr) 2020-05-27
US20150050697A1 (en) 2015-02-19
CN105722850B (zh) 2020-03-06

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